RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 48971
  • Symbol: Atpalpha
  • Description: Na pump alpha subunit
DataSource: http://genomernai.dkfz.de/v16/genedetails/48971

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 48971 FBgn0002921 Atpalpha DRSC14119 2.613518665 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 48971 FBgn0002921 Atpalpha DRSC14119 2.438731695 Increased lipid droplet area no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 48971 HFA14119 -0.9 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 48971 HFA14119 -0.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
48971 FBgn0002921 Atpalpha GH23483 sp none no library: DGC1
48971 FBgn0002921, FBgn0038145 Atpalpha, CG8863 GH27909 sp none no library: DGC2
48971 FBgn0031025, FBgn0002921 CG3878, Atpalpha LP11165 sp Increased G1 DNA content, decreased G2 DNA content yes library: DGC2
48971 FBgn0034441, FBgn0002921 CG10081, Atpalpha RE61138 sp none no library: DGC2
GR00048-A-2 48971 FBgn0031025, FBgn0002921 CG3878, Atpalpha np 1.298 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 48971 FBgn0031025, FBgn0002921 CG3878, Atpalpha np 458 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. BjÓ§rklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00134-A-1 48971 CG5670 Atpalpha 12330 np Lethal yes early pupal Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 48971 CG5670 Atpalpha np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal no Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00135-A-1 48971 CG5670 Atpalpha 12330 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 48971 CG5670 12330 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 48971 CG5670 Atpalpha 12330 0.259259259259259 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 48971 CG5670 12330 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00190-A-1 48971 FBgn0002921 Atpalpha 12330 -0.182 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00218-S 48971 CG5670 Atpalpha AMB22843 -0.05 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00238-S-1 48971 CG5670 ATPalpha 12330 sp Pupal lethal yes library: VDRC Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-2 48971 CG5670 ATPalpha 12330 sp none no library: VDRC Muscle maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue TARGET Mef2-GAL4 Climbing ability Negative geotaxis assay np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1 48971 CG5670 5670R np Decreased Chp protein expression no not detectable Chp level Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 48971 CG5670 Atpalpha 100619 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 48971 CG5670 Atpalpha 12330 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 48971 CG5670 Atpalpha 100619 0.4795588041198029 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 48971 FBgn0002921 Atpalpha DRSC14119 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 48971 CG5670 Atpalpha 100619 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00323-A-1 48971 FBgn0002921 Atpalpha 12330 np Decreased retina size Drosophila eye development The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development Marinho et al. 2013 23416177 Tissue eyeless-GAL4 Retina size Microscopy VDRC, NIG and TRiP Selected genes UAS-IR construct Visual inspection np Single RNAi screen; For double RNAi screen see "Viriato (Vito) interactions during eye development
GR00323-A-2 48971 FBgn0002921 Atpalpha 12330 np none Viriato (Vito) interactions during eye development The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development Marinho et al. 2013 23416177 Tissue eyeless-Gal4 Retina size Microscopy VDRC, NIG and TRiP Selected genes UAS-IR construct Genetic interaction score np Double RNAi screen; vito knockdown was archieved by expression of UAS-vitoRNAiKK in the female flies. For single RNAi screen see "Drosophila eye development
GR00335-A 48971 FBgn0002921 Atpalpha JF02910 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 48971 FBgn0002921 Atpalpha HMS00047 np F1 lethal F1 embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 48971 FBgn0002921 Atpalpha HMS00703 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
48971 np np HFA14119 -0.31 none fold change: 1.17
48971 FBgn0002921 np np sp none
GR00367-S 48971 CG5670 100619 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 48971 CG5670 100619 -0.337886586543906 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 48971 CG5670 BL33646 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 48971 CG5670 BL32913 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 48971 CG5670 BL28073 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 48971 CG5670 GD12330 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.