RNAi

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 1629
• Symbol: DBT
• Description: dihydrolipoamide branched chain transacylase E2

DataSource: http://genomernai.dkfz.de/v16/genedetails/1629

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00016-A	1629	1629	DBT	np	0.77	none		no		Wnt/beta-catenin pathway regulation	Bruton's tyrosine kinase revealed as a negative regulator of Wnt-beta-catenin signaling.	James et al.	2009	19471023	Cell line	RKO	Wnt/beta-catenin pathway reporter	Luminescence	rp	Selected genes	siRNA	Z-score	> 2
GR00018-A-0	1629		DBT	v2HS_151103	-0.82 (0.25)	Synthetic lethal with Ras		no		Synthetic lethal interaction with Ras	A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene.	Luo et al.	2009	19490893	Cell line	DLD-1	Synthetic lethal interaction with Ras	Micoarray hybridization	shRNA-mir (G. Hannon)		shRNA	Log2 diff MUT-WT (and P-value)	-0.7 (0.3)
GR00053-A	1629	NM_001918	DBT	np	sp	none		no		Genome stability	A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability.	Paulsen et al.	2009	19647519	Cell line	HeLa	gamma-H2AX phosphorylation and DNA content	Fluorescence	siARRAY human genome siRNA library	Genome-wide	siRNA	p-value	Complex criteria	Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A	1629	NM_001918	DBT	np	0.908	none		no		Combinatorial effect with paclitaxel	Synthetic lethal screen identification of chemosensitizer loci in cancer cells.	Whitehurst et al.	2007	17429401	Cell line	NCI-H1155	Viability (synthetic lethal)	ATP level	# G-005000-01	Genome-wide	siRNA	Paclitaxel/control ratio	Complex criteria	Additional information about 87 high-confidence hits
GR00056-A	1629	NM_001918	DBT	np	0.891	none		no		Melanogenesis	Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells.	Ganesan et al.	2008	19057677	Cell line	MNT-1	Melanin protein expression and viability	Absorbance and luminescence	rp	Genome-wide	siRNA	Normalized absorbance ratio	> 2 standard deviations below mean	Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1	1629	NM_001918	DBT	M-008279-00	1.11	none		no		Wnt/beta-catenin pathway regulation (1)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Z-score	> 4	Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2	1629	NM_001918	DBT	M-008279-00	sp	none		no		Wnt/beta-catenin pathway regulation (2)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Complex, SP	Complex criteria	Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00095-A	1629	NM_001918	DBT	np	np	none				p53 pathway components	A large-scale RNAi screen in human cells identifies new components of the p53 pathway	Berns et al.	2004	15042092	Cell line	BJ-TERT-tsLT fibroblasts	Proliferation	Colony-forming cell assay	Nki library	Genome-wide	shRNA	np	np
GR00098-A-1	1629	ENSG00000137992	DBT	ENSG00000137992	sp	none		no		Cell division (1)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Cell number and DNA content	Laser scanning cytometry	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00123-A	1629	NM_001918	DBT	160233, 160232, 160231	np	none				Combinatorial effect with nutlin-3	An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors	Brummelkamp et al.	2006	16474381	Cell line	MCF-7	Viability	Fluoresence	Nki library	Genome-wide	shRNA	log2 ratio	np
GR00133-A-1	1629	1629	DBT	np	-0.236952	none		no		Salmonella enterica subspecies 1 serovar Typhimurium invasion (1)	RNAi screen of Salmonella invasion shows role of COPI in membrane targeting of cholesterol and Cdc42.	Misselwitz et al.	2011	21407211	Cell line	HeLa	Gentamycin protection invasion assay	Fluorescence	Druggable genome library V2.0	Druggable genes	siRNA	log2 median	Complex criteria
GR00149-A-1	1629	1629	DBT	10591	32.61	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	10591	45.67	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106614	68.36	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106614	58.16	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106615	9.77	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106615	-17.16	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106616	-26.01	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	1629	1629	DBT	106616	-18.82	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00151-A-1	1629	ENSG00000137992	DBT	np	-0.343	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.	SÅ‚abicki et al.	2010	20613862	Cell line	HeLa	(HR-DSBR) DR-GFP reporter	Flow cytometry	Custom-made	Genome-wide	esiRNA	Z-score	< -2 OR > 2
GR00180-A-1	1629	1629	DBT	PL-50012	0.803	none		no		Hepatitis C virus replication (1)	A functional genomic screen identifies cellular cofactors of hepatitis C virus replication.	Tai et al.	2009	19286138	Cell line	Huh7/Rep-Feo	HCV replicon RNA copy number	Luminescence	siARRAY Human Genome siRNA Library	Genome-wide	siRNA	q-value	Complex criteria
GR00184-A-1	1629	NM_001918	DBT	M-008279-00	-0.892353141086129	none		no		Self-renewal and pluripotency in human embryonic stem cells (1)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	SMARTpool siRNA library	Genome-wide	siRNA	Z-score	< -2
GR00195-A-1	1629	1629	DBT	np	sp	Decreased influenza A/WSN/33 replication		yes		Influenza virus infection (1)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and 293T	Number of influenza A H1N1 (A/WSN/33) infected cells and viral polymerase protein expression	Fluorescence and luminescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Genome-wide	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00195-A-2	1629	1629	DBT	Hs_DBT_2, Hs_DBT_4, Hs_DBT_5, Hs_DBT_6	sp	Decreased influenza A H1N1 (A/WSN/33) virus numbers		yes		Influenza virus infection (2)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and MDCK	Number of influenza A H1N1 (A/WSN/33) viruses	Fluorescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Selected genes	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00195-A-3	1629	1629	DBT	Hs_DBT_2, Hs_DBT_4, Hs_DBT_5, Hs_DBT_6	sp	Decreased influenza A H1N1 (A/Hamburg/04/2009) virus numbers		no		Influenza virus infection (3)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and MDCK	Number of influenza A H1N1 (A/Hamburg/04/2009) viruses	Fluorescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Selected genes	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00196-A-1	1629	ENSG00000137992		np	sp	none		no		TP53 interactions (1)	A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.	Krastev et al.	2011	21642980	Cell line	HCT116 ( wildtype and TP53 knockout)	TP53 protein expression and viability	Fluorescence	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00197-A-1	1629	1629	DBT	M-008279-00	0.656646718	none		no		Human papillomavirus oncogene expression regulation (1)	Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.	Smith et al.	2010	20133580	Cell line	C33A/BE2/18LCR c4	HPV18 LCR reporter activity	Luminescence	Human siGENOME SMARTpool library	Genome-wide	siRNA	Z-score	>	Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00198-A-2	1629	1629	DBT	M-008279-00	np	Decreased number of cells in monopolar arrest with EMD534085 (a Kinesin-5 inhibitor)		no	number of confimed duplexes: 2	Combinatorial effect with EMD534085, a Kinesin-5 inhibitor (2)	An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor.	Tsui et al.	2009	19802393	Cell line	HeLa	H2B protein expression	Fluorescence	siARRAY siRNA Library	Selected genes	siRNA	Ratio monopolar to total nuclei	> 2 standard deviations below mean for >	Additional information about the primary genome-wide screen
	1629		DBT	np	np	Decreased viability in glioblastoma multiforme (GBM) lineage		no	GBM: no filter, 2nd best
GR00236-A-1	1629	1629	DBT	M-008279-00	1.1801611349025	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response.	Adamson et al.	2012	22344029	Cell line	DR-U2OS	(HR-DSBR) DR-GFP reporter and DNA content	Fluorescence	Human siGENOME siRNA (G-005000-05)	Genome-wide	siRNA	Relative HR ratio	< ~0.4 OR > 1.88	Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1	1629	NM_001918	DBT	M-008279-00	0.73	none		yes		TRAIL-induced apoptosis (1)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Z-score	> 4	Author-submitted data
GR00240-S-2	1629	NM_001918	DBT	M-008279-00	0.06	none		no	Z-score 0.973	TRAIL-induced apoptosis (2)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability (synthetic lethal)	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Differential score	> 3.6 AND viability Z-score < 4	Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00241-A-1	1629	1629	DBT	Hs_DBT_4, Hs_DBT_2, Hs_DBT_5, Hs_DBT_6	0.002916119	Decreased vesicular stomatitis virus (VSV) infection		yes	set AB_1: Hs_DBT_4, set AB_2: Hs_DBT_2, set CD_1: Hs_DBT_5, set CD_2: Hs_DBT_6	Negative-strand RNA virus infection (1) - vesicular stomatitis virus (VSV)	RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses.	Panda et al.	2011	22065774	Cell line	HeLa	VSV-eGFP protein expression and DNA content	Fluorescence	Genomic siRNA library v1.0	Genome-wide	siRNA	p-value	< 0.01	Ribosomal protein genes were excluded from hit list.
GR00241-A-2	1629	1629	DBT	np	np	none		no		Negative-strand RNA virus infection (2) - vesicular stomatitis virus (VSV)	RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses.	Panda et al.	2011	22065774	Cell line	HeLa	VSV-eGFP protein expression and DNA content	Fluorescence	ON-TARGETplus	Selected genes	siRNA	p-value	<
GR00242-A-1	1629	NM_001918	DBT	np	sp	none		no		Selective autophagy regulation (1)	Image-based genome-wide siRNA screen identifies selective autophagy factors.	Orvedahl et al.	2011	22020285	Cell line	HeLa/GFP-LC3	Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression	Fluorescence	siGenome	Genome-wide	siRNA	Z-score	Complex criteria
GR00247-A-1	1629		DBT	np	sp	none			rank: 10751	Regulation of FOXO1 nuclear localization (1)	Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain.	Senapedis et al.	2011	21460183	Cell line	U2OS	EGFP-FOXO1a protein expression and DNA content	Fluorescence	Human Genome library	Genome-wide	siRNA	Complex, sp	Complex criteria
GR00249-S	1629	1629	DBT	J-008279-05	0.36997	none		no	number of cells compared to control (%): 96.40	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	1629	1629	DBT	M-008279-01	-0.9481	none		no	number of cells compared to control (%): 74.28	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	1629	1629	DBT	s3956	-0.14322	none		no	number of cells compared to control (%): 69.43	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	1629	1629	DBT	s3957	-0.31454	none		no	number of cells compared to control (%): 94.84	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	1629	1629	DBT	s3958	0.50768	none		no	number of cells compared to control (%): 81.09	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00253-A	1629	NM_001918	DBT	np	0.262	none				hepcidin regulation	Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling.	Mleczko-Sanecka et al.	2014	24385536	Cell line	Huh7	hepcidin::fluc mRNA expression	Luminescence	siGenome siARRAY SMARTpool	Genome-wide	siRNA	Z-score	>	Cutoff <
GR00255-A-1	1629	1629	DBT	TRCN0000025796, TRCN0000025813, TRCN0000025837, TRCN0000025838, TRCN0000025870	-0.1994917	none				Negative genetic interactions (1)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2	1629	1629	DBT	TRCN0000025796, TRCN0000025813, TRCN0000025837, TRCN0000025838, TRCN0000025870	-0.048100234	none				Negative genetic interactions (2)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3	1629	1629	DBT	TRCN0000025796, TRCN0000025813, TRCN0000025837, TRCN0000025838, TRCN0000025870	0.039406734	none				Negative genetic interactions (3)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4	1629	1629	DBT	TRCN0000025796, TRCN0000025813, TRCN0000025837, TRCN0000025838, TRCN0000025870	0.24914221	none				Negative genetic interactions (4)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5	1629	1629	DBT	TRCN0000025796, TRCN0000025813, TRCN0000025837, TRCN0000025838, TRCN0000025870	0.100130815	none				Negative genetic interactions (5)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -0.8	HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00293-A	1629		DBT	np	0.235	none				Combinatorial effect with paclitaxel	Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death	Sinnott et al.	2014	24860162	Cell line	HCC366	Viability	Luminescence	Thermo-Fisher	Genome-wide	siRNA	Z-score	< -2.5	Final hits according to the author are indicated in the comment.
GR00297-A	1629		DBT	TRCN0000221423	0.29	none				Glucose limitation response	Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides	Birsoy et al.	2014	24670634	Cell line	Jurkat	shRNA abundance in high and low glucose	Deep sequencing	TRC	Selected genes	shRNA	Log2 ratio	< -0.75
GR00297-A	1629		DBT	TRCN0000221425	0.38	none				Glucose limitation response	Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides	Birsoy et al.	2014	24670634	Cell line	Jurkat	shRNA abundance in high and low glucose	Deep sequencing	TRC	Selected genes	shRNA	Log2 ratio	< -0.75
GR00297-A	1629		DBT	TRCN0000221426	-0.12	none				Glucose limitation response	Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides	Birsoy et al.	2014	24670634	Cell line	Jurkat	shRNA abundance in high and low glucose	Deep sequencing	TRC	Selected genes	shRNA	Log2 ratio	< -0.75
GR00297-A	1629		DBT	TRCN0000221424	0.05	none				Glucose limitation response	Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides	Birsoy et al.	2014	24670634	Cell line	Jurkat	shRNA abundance in high and low glucose	Deep sequencing	TRC	Selected genes	shRNA	Log2 ratio	< -0.75
GR00297-A	1629		DBT	TRCN0000221427	0.02	none				Glucose limitation response	Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides	Birsoy et al.	2014	24670634	Cell line	Jurkat	shRNA abundance in high and low glucose	Deep sequencing	TRC	Selected genes	shRNA	Log2 ratio	< -0.75
GR00300-A	1629		DBT	TRCN0000025870, TRCN0000025796, TRCN0000025813, TRCN0000025838, TRCN0000025837	0	none				Combinatorial effect with RAF inhibitor PLX4720	A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition.	Whittaker et al.	2013	23288408	Cell line	A375	shRNA abundance	Sequencing	TRC	Genome-wide	shRNA	Number of shRNAs ranked Top1000	> 2	The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A	1629	NM_001918	DBT	np	-0.02	none				Clear cell renal cell carcinoma (ccRCC) survival regulation	Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target	Gerlinger et al.	2012	22362593	Cell line	VHL-deficient RCC4	Proliferation and Viability	Fluorescence	np	Genome-wide	siRNA	Z-score	<	In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1	1629	1629	DBT	np	-6.93	Decreased Sindbis virus (SINV) infection				Sindbis virus (SINV) infection (1)	Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry	Ooi et al.	2013	24367265	Cell line	U2OS	Sindbis virus (SINV) reporter	Luminescence	Ambion Silencer V3	Genome-wide	siRNA	Z-score	< -3 OR > 2
	1629	1629	DBT	np	41.9	none			83,5% viability
GR00313-A	1629	NM_001918	DBT	np	0.44	none				TNF-alpha pathway regulation	A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling.	Nickles et al.	2012	22733992	Cell line	HEK293T	NFkappaB pathway reporter	Luminescence	Qiagen	Genome-wide	siRNA	Z-score	<	Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
GR00318-A	1629	1629	DBT	np	1.232	none			siRNA set: druggable genome	Huntingtin toxicity	A Genome-Scale RNA–Interference Screen Identifies RRAS Signaling as a Pathologic Feature of Huntington’s Disease	Miller et al.	2012	23209424	Cell line	HEK293T	Caspase 3/7 activity	Fluorescence	Dharmacon	Selected genes	siRNA	Sum of normalized caspase 3/7 activity mean and standard error	< 0.683	HEK293T cells were cotransfected with mutant Huntingtin fused to GFP (Htt1-558141Q-GFP).
GR00327-A	1629	1629	IL10RA	CLL-H-014005	-0.5242594469765883	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A	1629	1629	IL10RA	CLL-H-014004	0.980115024500465	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A	1629	1629	IL10RA	CLL-H-014003	-1.2649860620595559	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A	1629	1629	IL10RA	CLL-H-014006	0.5342234367105537	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A	1629	1629	IL10RA	CLL-H-014002	0.5123110260257443	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A	1629	1629	IL10RA	CLL-H-014001	0.166010860651272	none				Tumor formation	An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation	Wolf et al.	2013	24292671	Cell line	SUM-149	shRNA abundance	Next-generation sequencing	Decipher library module 1	Selected genes	shRNA	Z-score	> 2.24	Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00343-S	1629	1629	DBT	TRCN0000025813	0.16499999999999915	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	1629	1629	DBT	TRCN0000025870	-0.2975000000000012	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	1629	1629	DBT	TRCN0000025838	0.5475000000000012	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	1629	1629	DBT	TRCN0000025837	-0.3050000000000015	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	1629	1629	DBT	TRCN0000025796	1.5649999999999995	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00360-A-1	1629	ENSG00000137992	DBT	106615	0.8439666666666671	Mildly decreased CFP-tsO45G cell surface transport				Secretory transport (1)	Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway	Simpson et al.	2012	22660414	Cell line	HeLa Kyoto	CFP-tsO45G cell surface transport	Fluorescence	Ambion	Genome-wide	siRNA	Deviation score	< - 1 OR > 0.75 (mild: 0.75 - 1.0; strong: > 1.0)	For validation screen see Secretory transport (2).
GR00360-A-1	1629	ENSG00000137992	DBT	106616	np	No cells				Secretory transport (1)	Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway	Simpson et al.	2012	22660414	Cell line	HeLa Kyoto	CFP-tsO45G cell surface transport	Fluorescence	Ambion	Genome-wide	siRNA	Deviation score	< - 1 OR > 0.75 (mild: 0.75 - 1.0; strong: > 1.0)	For validation screen see Secretory transport (2).
	1629	1629	DBT		-0.02	none
GR00376-A-1	1629	1629	DBT		0.045651932	none				Mitigators of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2	1629	1629	DBT		0.651401258	none				Sensitizers of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
GR00378-A	1629		DBT		-0.973968426	none				Poliovirus vaccine production	Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game	van der Sanden et al.	2015	26581994	Cell line	HEp-2C	Infection with Attenuated Poliovirus	ELISA	Dharmacon	Genome-wide	siRNA	Z-score	>	For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol
	1629	NM_001918	DBT		37.472	none
GR00386-A-1	1629	1629	DBT		76.5447852914618	none				NOD2 stimulation by MDP	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	Viability	Luminescence	Dharmacon	Genome-wide	siRNA	Percentage growth	Decreased: <70, increased: >120	Reagent IDs not provided
GR00386-A-2	1629	1629	DBT		-11.9	none				MDP-induced IL-8 secretion	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	IL-8 secretion	ELISA	Dharmacon	Genome-wide	siRNA	Percent inhibition of IL-8 secretion	Increased: <-300, Decreased: >60	Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided