RNAi

Gene Info

  • Species: Human (Homo sapiens)
  • GeneID: 26173
  • Symbol: INTS1
  • Description: integrator complex subunit 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/26173

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00017-A-0 26173 INTS1 INTS1_siRNA-Single-1 14.78 Wnt reporter downregulated no Wnt signaling New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening. Major et al. 2008 19001663 Cell line DLD-1 Wnt signaling Dual luciferase Custom-made library siRNA Percentage Wnt reporter activity np
GR00017-A-0 26173 INTS1 INTS1_siRNA-Single-3 28.92 Wnt reporter downregulated no Wnt signaling New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening. Major et al. 2008 19001663 Cell line DLD-1 Wnt signaling Dual luciferase Custom-made library siRNA Percentage Wnt reporter activity np
GR00057-A-1 26173 XM_291222 DKFZP586J0619 M-025278-00 -0.63 none no Wnt/beta-catenin pathway regulation (1) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Z-score > 4 Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2 26173 XM_291222 DKFZP586J0619 M-025278-00 sp none no Wnt/beta-catenin pathway regulation (2) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Complex, SP Complex criteria Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00098-A-1 26173 ENSG00000164860 INTS1 ENSG00000164860 sp none no Cell division (1) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell number and DNA content Laser scanning cytometry rp Genome-wide esiRNA Complex, sp Complex criteria
GR00098-A-1 26173 ENSG00000164880 INTS1 ENSG00000164880 sp none no Cell division (1) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell number and DNA content Laser scanning cytometry rp Genome-wide esiRNA Complex, sp Complex criteria
GR00151-A-1 26173 ENSG00000164880 INTS1 np -0.251 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. SÅ‚abicki et al. 2010 20613862 Cell line HeLa (HR-DSBR) DR-GFP reporter Flow cytometry Custom-made Genome-wide esiRNA Z-score < -2 OR > 2
GR00165-A 26173 NM_001080453 INTS1 M-025278-00 np Big cells no HeLa cell morphology Clustering phenotype populations by genome-wide RNAi and multiparametric imaging. Fuchs et al. 2010 20531400 Cell line HeLa Cell morphology Fluorescence siGENOME Genome-wide siRNA Complex, sp np
GR00180-A-1 26173 26173 INTS1 PL-50056 0.752 none no Hepatitis C virus replication (1) A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. Tai et al. 2009 19286138 Cell line Huh7/Rep-Feo HCV replicon RNA copy number Luminescence siARRAY Human Genome siRNA Library Genome-wide siRNA q-value Complex criteria
GR00184-A-1 26173 XM_291222 INTS1 M-025278-00 0.737234495664166 none no Self-renewal and pluripotency in human embryonic stem cells (1) A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Chia et al. 2010 20953172 Cell line hESC H1 POU5F1 protein expression Fluorescence SMARTpool siRNA library Genome-wide siRNA Z-score < -2
GR00196-A-1 26173 ENSG00000164880 np sp none no TP53 interactions (1) A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Krastev et al. 2011 21642980 Cell line HCT116 ( wildtype and TP53 knockout) TP53 protein expression and viability Fluorescence rp Genome-wide esiRNA Complex, sp Complex criteria
GR00197-A-1 26173 26173 DKFZP586J0619 M-025278-00 4.944919702 Increased HPV18 LCR reporter activity yes deconvoluted validated siRNAs: 3 Human papillomavirus oncogene expression regulation (1) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Genome-wide siRNA Z-score > Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00197-A-3 26173 26173 INTS1 D-025278-03 1.786 none yes Human papillomavirus oncogene expression regulation (3) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-3 26173 26173 INTS1 D-025278-02 6.458 Increased HPV18 LCR reporter activity yes Human papillomavirus oncogene expression regulation (3) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-3 26173 26173 INTS1 D-025278-01 7.623 Increased HPV18 LCR reporter activity yes Human papillomavirus oncogene expression regulation (3) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-3 26173 26173 INTS1 D-025278-04 8.927 Increased HPV18 LCR reporter activity yes Human papillomavirus oncogene expression regulation (3) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-4 26173 26173 INTS1 D-025278-04 4.6 Increased HPV18 LCR reporter activity yes Human papillomavirus oncogene expression regulation (4) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/16E2/18LCR clone 1 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-4 26173 26173 INTS1 D-025278-02 1.611 none yes Human papillomavirus oncogene expression regulation (4) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/16E2/18LCR clone 1 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-4 26173 26173 INTS1 D-025278-03 -0.071 none yes Human papillomavirus oncogene expression regulation (4) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/16E2/18LCR clone 1 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-4 26173 26173 INTS1 D-025278-01 0.301 none yes Human papillomavirus oncogene expression regulation (4) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/16E2/18LCR clone 1 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Selected genes siRNA Z-score np Author-reviewed data
GR00197-A-5 26173 26173 INTS1 D-025278-03 -2.614 Decreased BPV1 E2 protein expression no Human papillomavirus oncogene expression regulation (5) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 BPV1 E2 protein expression Fluorescence Human siGENOME SMARTpool library Selected genes siRNA Z-score < Author-reviewed data
GR00197-A-5 26173 26173 INTS1 D-025278-02 -1.543 Decreased BPV1 E2 protein expression no Human papillomavirus oncogene expression regulation (5) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 BPV1 E2 protein expression Fluorescence Human siGENOME SMARTpool library Selected genes siRNA Z-score < Author-reviewed data
GR00197-A-5 26173 26173 INTS1 D-025278-04 -1.258 none no Human papillomavirus oncogene expression regulation (5) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 BPV1 E2 protein expression Fluorescence Human siGENOME SMARTpool library Selected genes siRNA Z-score < Author-reviewed data
GR00197-A-5 26173 26173 INTS1 D-025278-01 -2.29 Decreased BPV1 E2 protein expression no Human papillomavirus oncogene expression regulation (5) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 BPV1 E2 protein expression Fluorescence Human siGENOME SMARTpool library Selected genes siRNA Z-score < Author-reviewed data
GR00236-A-1 26173 26173 INTS1 M-025278-00 0.28118849598659 Decreased homologous recombination repair frequency yes Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Human siGENOME siRNA (G-005000-05) Genome-wide siRNA Relative HR ratio < ~0.4 OR > 1.88 Cutoff values correspond 2 standard deviations from the screen-wide mean
26173 26173 INTS1 D-025278-01 1.19 none yes
26173 26173 INTS1 D-025278-02 1.34 none yes
26173 26173 INTS1 D-025278-03 1.48 none yes
26173 26173 INTS1 D-025278-04 0.47 Decreased homologous recombination repair frequency yes weak cutoff
GR00236-A-3 26173 26173 INTS1 s25211 0.62 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (3) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Silencer Select Selected genes siRNA Relative HR ratio Strong: < ~0.4; weak: < ~0.59 Cutoff values correspond 2 standard deviations from the screen-wide mean (strong) or 1.5 SD (weak)
GR00236-A-3 26173 26173 INTS1 s25212 1.32 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (3) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Silencer Select Selected genes siRNA Relative HR ratio Strong: < ~0.4; weak: < ~0.59 Cutoff values correspond 2 standard deviations from the screen-wide mean (strong) or 1.5 SD (weak)
GR00236-A-3 26173 26173 INTS1 s25213 0.29 Decreased homologous recombination repair frequency no strong cutoff Homologous recombination DNA double-strand break repair (HR-DSBR) (3) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Silencer Select Selected genes siRNA Relative HR ratio Strong: < ~0.4; weak: < ~0.59 Cutoff values correspond 2 standard deviations from the screen-wide mean (strong) or 1.5 SD (weak)
GR00240-S-1 26173 XM_291222 DKFZP586J0619 M-025278-00 5.55 Decreased viability yes TRAIL-induced apoptosis (1) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability Luminescence SMART-pool siRNA Genome-wide siRNA Z-score > 4 Author-submitted data
GR00240-S-2 26173 XM_291222 DKFZP586J0619 M-025278-00 np none no Z-score 6.5475; viability Z-score > 4 TRAIL-induced apoptosis (2) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability (synthetic lethal) Luminescence SMART-pool siRNA Genome-wide siRNA Differential score > 3.6 AND viability Z-score < 4 Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00247-A-1 26173 INTS1 np sp none rank: 1756 Regulation of FOXO1 nuclear localization (1) Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. Senapedis et al. 2011 21460183 Cell line U2OS EGFP-FOXO1a protein expression and DNA content Fluorescence Human Genome library Genome-wide siRNA Complex, sp Complex criteria
GR00249-S 26173 26173 DKFZP586J0619 J-025278-17 0.07494 none no number of cells compared to control (%): 70.50 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 26173 26173 DKFZP586J0619 M-025278-01 1.28419 Increased vaccinia virus (VACV) infection no number of cells compared to control (%): 71.28 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 26173 26173 INTS1 s25211 -1.40387 none no number of cells compared to control (%): 84.64 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 26173 26173 INTS1 s25212 0.8767 none no number of cells compared to control (%): 91.60 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 26173 26173 INTS1 s25213 2.45606 Increased vaccinia virus (VACV) infection no number of cells compared to control (%): 83.77 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
26173 26173 DKFZP586J0619 M-025278-01 -2.760305327672917 Decreased interferon-beta reporter expression
26173 26173 INTS1 np 88.7 Inconclusive 43,5% viability
GR00376-A-1 26173 26173 INTS1 0.120115728 none Mitigators of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2 26173 26173 INTS1 0.089620099 none Sensitizers of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
GR00386-A-1 26173 26173 INTS1 40.4775629016917 Decreased viability NOD2 stimulation by MDP A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 Viability Luminescence Dharmacon Genome-wide siRNA Percentage growth Decreased: <70, increased: >120 Reagent IDs not provided
GR00386-A-2 26173 26173 INTS1 -505.2 Increased IL-8 secretion MDP-induced IL-8 secretion A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 IL-8 secretion ELISA Dharmacon Genome-wide siRNA Percent inhibition of IL-8 secretion Increased: <-300, Decreased: >60 Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided