RNAi

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 5429
• Symbol: POLH
• Description: DNA polymerase eta

DataSource: http://genomernai.dkfz.de/v16/genedetails/5429

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00053-A	5429	NM_006502	POLH	np	sp	none		no		Genome stability	A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability.	Paulsen et al.	2009	19647519	Cell line	HeLa	gamma-H2AX phosphorylation and DNA content	Fluorescence	siARRAY human genome siRNA library	Genome-wide	siRNA	p-value	Complex criteria	Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A	5429	NM_006502	POLH	np	0.912	none		no		Combinatorial effect with paclitaxel	Synthetic lethal screen identification of chemosensitizer loci in cancer cells.	Whitehurst et al.	2007	17429401	Cell line	NCI-H1155	Viability (synthetic lethal)	ATP level	# G-005000-01	Genome-wide	siRNA	Paclitaxel/control ratio	Complex criteria	Additional information about 87 high-confidence hits
GR00056-A	5429	NM_006502	POLH	np	0.859	none		no		Melanogenesis	Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells.	Ganesan et al.	2008	19057677	Cell line	MNT-1	Melanin protein expression and viability	Absorbance and luminescence	rp	Genome-wide	siRNA	Normalized absorbance ratio	> 2 standard deviations below mean	Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1	5429	NM_006502	POLH	M-006454-00	-0.04	none		no		Wnt/beta-catenin pathway regulation (1)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Z-score	> 4	Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2	5429	NM_006502	POLH	M-006454-00	sp	none		no		Wnt/beta-catenin pathway regulation (2)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Complex, SP	Complex criteria	Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00095-A	5429	NM_006502	POLH	np	np	none				p53 pathway components	A large-scale RNAi screen in human cells identifies new components of the p53 pathway	Berns et al.	2004	15042092	Cell line	BJ-TERT-tsLT fibroblasts	Proliferation	Colony-forming cell assay	Nki library	Genome-wide	shRNA	np	np
GR00098-A-1	5429	ENSG00000170734	POLH	ENSG00000170734	sp	none		no		Cell division (1)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Cell number and DNA content	Laser scanning cytometry	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00101-A-4	5429	NM_006502	POLH	np	2.5	Decreased viability with cisplatin		no		Combinatorial effect with anticancer drugs (4): cisplatin	Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions.	Bartz et al.	2006	17000754	Cell line	HeLa	Viability (synthetic lethal)	Alamar Blue	rp	Selected genes	siRNA	Fold EC50 shift	>	Additional information about the primary genome-wide screen
GR00123-A	5429	NM_006502	POLH	164141, 164143, 164142	np	none				Combinatorial effect with nutlin-3	An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors	Brummelkamp et al.	2006	16474381	Cell line	MCF-7	Viability	Fluoresence	Nki library	Genome-wide	shRNA	log2 ratio	np
GR00133-A-1	5429	5429	POLH	np	0.009566	none		no		Salmonella enterica subspecies 1 serovar Typhimurium invasion (1)	RNAi screen of Salmonella invasion shows role of COPI in membrane targeting of cholesterol and Cdc42.	Misselwitz et al.	2011	21407211	Cell line	HeLa	Gentamycin protection invasion assay	Fluorescence	Druggable genome library V2.0	Druggable genes	siRNA	log2 median	Complex criteria
GR00149-A-1	5429	5429	POLH	17972	np	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	17972	53.52	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119730	-4.35	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119730	-42.49	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119731	24.38	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119731	-28.31	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119732	-36.35	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	5429	5429	POLH	119732	-49.55	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00151-A-1	5429	ENSG00000170734	POLH	np	-0.227	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.	SÅ‚abicki et al.	2010	20613862	Cell line	HeLa	(HR-DSBR) DR-GFP reporter	Flow cytometry	Custom-made	Genome-wide	esiRNA	Z-score	< -2 OR > 2
GR00180-A-1	5429	5429	POLH	PL-50013	0.918	none		no		Hepatitis C virus replication (1)	A functional genomic screen identifies cellular cofactors of hepatitis C virus replication.	Tai et al.	2009	19286138	Cell line	Huh7/Rep-Feo	HCV replicon RNA copy number	Luminescence	siARRAY Human Genome siRNA Library	Genome-wide	siRNA	q-value	Complex criteria
GR00184-A-1	5429	NM_006502	POLH	M-006454-00	-2.58591643194893	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (1)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	SMARTpool siRNA library	Genome-wide	siRNA	Z-score	< -2
GR00184-A-2	5429	NM_006502	POLH	D-006454-06	0.363181517301531	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	5429	NM_006502	POLH	D-006454-05	0.595595312759842	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	5429	NM_006502	POLH	D-006454-08	0.630127917038361	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	5429	NM_006502	POLH	D-006454-07	0.857799342835837	none		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	5429	NM_006502	POLH	D-006454-05	0.347760351981366	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	5429	NM_006502	POLH	D-006454-07	0.609873333370071	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	5429	NM_006502	POLH	D-006454-06	0.319274491647753	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	5429	NM_006502	POLH	D-006454-08	0.412258012961699	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	5429	NM_006502	POLH	D-006454-08	0.350619318594803	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	5429	NM_006502	POLH	D-006454-06	0.253075960194107	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	5429	NM_006502	POLH	D-006454-05	0.295273842410816	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	5429	NM_006502	POLH	D-006454-07	0.535154655442517	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	5429	NM_006502	POLH	D-006454-06	0.715080007499656	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	5429	NM_006502	POLH	D-006454-08	0.921651719284004	none		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	5429	NM_006502	POLH	D-006454-05	0.768214735185127	none		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	5429	NM_006502	POLH	D-006454-07	0.97669942128965	none		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	5429	NM_006502	POLH	D-006454-05	0.872351005620663	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	5429	NM_006502	POLH	D-006454-06	0.979709777797511	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	5429	NM_006502	POLH	D-006454-08	0.850373125304935	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	5429	NM_006502	POLH	D-006454-07	0.855602402373815	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	5429	NM_006502	POLH	D-006454-05	0.749129988417929	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	5429	NM_006502	POLH	D-006454-08	1.1156212346699	none		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	5429	NM_006502	POLH	D-006454-06	0.804126554145626	none		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	5429	NM_006502	POLH	D-006454-07	1.23167968760945	none		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	5429	NM_006502	POLH	D-006454-06	0.835295707346662	none		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	5429	NM_006502	POLH	D-006454-05	0.688469148457707	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	5429	NM_006502	POLH	D-006454-08	0.959170342763397	none		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	5429	NM_006502	POLH	D-006454-07	1.0255886730506	none		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00196-A-1	5429	ENSG00000170734		np	sp	none		no		TP53 interactions (1)	A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.	Krastev et al.	2011	21642980	Cell line	HCT116 ( wildtype and TP53 knockout)	TP53 protein expression and viability	Fluorescence	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00197-A-1	5429	5429	POLH	M-006454-00	0.378072263	none		no		Human papillomavirus oncogene expression regulation (1)	Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.	Smith et al.	2010	20133580	Cell line	C33A/BE2/18LCR c4	HPV18 LCR reporter activity	Luminescence	Human siGENOME SMARTpool library	Genome-wide	siRNA	Z-score	>	Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00236-A-1	5429	5429	POLH	M-006454-00	1.13488135407746	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response.	Adamson et al.	2012	22344029	Cell line	DR-U2OS	(HR-DSBR) DR-GFP reporter and DNA content	Fluorescence	Human siGENOME siRNA (G-005000-05)	Genome-wide	siRNA	Relative HR ratio	< ~0.4 OR > 1.88	Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1	5429	NM_006502	POLH	M-006454-00	-0.68	none		yes		TRAIL-induced apoptosis (1)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Z-score	> 4	Author-submitted data
GR00240-S-2	5429	NM_006502	POLH	M-006454-00	0.71	none		no	Z-score 0.0305	TRAIL-induced apoptosis (2)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability (synthetic lethal)	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Differential score	> 3.6 AND viability Z-score < 4	Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1	5429	NM_006502	POLH	np	sp	none		no		Selective autophagy regulation (1)	Image-based genome-wide siRNA screen identifies selective autophagy factors.	Orvedahl et al.	2011	22020285	Cell line	HeLa/GFP-LC3	Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression	Fluorescence	siGenome	Genome-wide	siRNA	Z-score	Complex criteria
GR00247-A-1	5429		POLH	np	sp	none			rank: 15333	Regulation of FOXO1 nuclear localization (1)	Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain.	Senapedis et al.	2011	21460183	Cell line	U2OS	EGFP-FOXO1a protein expression and DNA content	Fluorescence	Human Genome library	Genome-wide	siRNA	Complex, sp	Complex criteria
GR00249-S	5429	5429	POLH	J-006454-09	-0.99326	none		no	number of cells compared to control (%): 99.93	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	5429	5429	POLH	M-006454-01	-1.1301	Decreased viability		no	number of cells compared to control (%): 36.66	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	5429	5429	POLH	s10790	-0.37144	none		no	number of cells compared to control (%): 71.19	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	5429	5429	POLH	s10791	0.56186	none		no	number of cells compared to control (%): 90.17	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	5429	5429	POLH	s10792	-1.03979	none		no	number of cells compared to control (%): 96.25	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00250-A-3	5429	5429	POLH	D-006454-05	sp	Increased viability with MLN4924 (a NAE inhibitor)			91 nM MLN4924; set: 320DDR; final hit	Combinatorial effect with MLN4924, a NAE inhibitor (3)	Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924.	Blank et al.	2013	23100467	Cell line	HCT116	Viability (synthetic lethal)	Luminescence	np	Selected and random genes	siRNA	Complex, sp	Complex criteria
GR00250-A-3	5429	5429	POLH	D-006454-06	sp	Synthetic lethal with MLN4924 (a NAE inhibitor)			91 nM MLN4924; set: 320DDR; final hit	Combinatorial effect with MLN4924, a NAE inhibitor (3)	Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924.	Blank et al.	2013	23100467	Cell line	HCT116	Viability (synthetic lethal)	Luminescence	np	Selected and random genes	siRNA	Complex, sp	Complex criteria
GR00250-A-3	5429	5429	POLH	D-006454-07	sp	Increased viability with MLN4924 (a NAE inhibitor)			91 nM MLN4924; set: 320DDR; final hit	Combinatorial effect with MLN4924, a NAE inhibitor (3)	Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924.	Blank et al.	2013	23100467	Cell line	HCT116	Viability (synthetic lethal)	Luminescence	np	Selected and random genes	siRNA	Complex, sp	Complex criteria
GR00250-A-3	5429	5429	POLH	D-006454-08	sp	Increased viability with MLN4924 (a NAE inhibitor)			91 nM MLN4924; set: 320DDR; final hit	Combinatorial effect with MLN4924, a NAE inhibitor (3)	Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924.	Blank et al.	2013	23100467	Cell line	HCT116	Viability (synthetic lethal)	Luminescence	np	Selected and random genes	siRNA	Complex, sp	Complex criteria
GR00253-A	5429	NM_006502	POLH	np	0.935	none				hepcidin regulation	Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling.	Mleczko-Sanecka et al.	2014	24385536	Cell line	Huh7	hepcidin::fluc mRNA expression	Luminescence	siGenome siARRAY SMARTpool	Genome-wide	siRNA	Z-score	>	Cutoff <
GR00255-A-1	5429	5429	POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	-0.3316484	none				Negative genetic interactions (1)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2	5429	5429	POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	-0.728550292	none				Negative genetic interactions (2)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3	5429	5429	POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	-0.632842488	none				Negative genetic interactions (3)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4	5429	5429	POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	-1.292716082	Negative genetic interaction between PTTG1-/- and PTTG1+/+				Negative genetic interactions (4)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5	5429	5429	POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	0.331554281	none				Negative genetic interactions (5)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -0.8	HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00293-A	5429		POLH	np	0.902	none				Combinatorial effect with paclitaxel	Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death	Sinnott et al.	2014	24860162	Cell line	HCC366	Viability	Luminescence	Thermo-Fisher	Genome-wide	siRNA	Z-score	< -2.5	Final hits according to the author are indicated in the comment.
GR00300-A	5429		POLH	TRCN0000053008, TRCN0000053009, TRCN0000053010, TRCN0000053011, TRCN0000053012	0	none				Combinatorial effect with RAF inhibitor PLX4720	A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition.	Whittaker et al.	2013	23288408	Cell line	A375	shRNA abundance	Sequencing	TRC	Genome-wide	shRNA	Number of shRNAs ranked Top1000	> 2	The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A	5429	NM_006502	POLH	np	-0.74	none				Clear cell renal cell carcinoma (ccRCC) survival regulation	Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target	Gerlinger et al.	2012	22362593	Cell line	VHL-deficient RCC4	Proliferation and Viability	Fluorescence	np	Genome-wide	siRNA	Z-score	<	In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1	5429	5429	POLH	np	0.28	none				Sindbis virus (SINV) infection (1)	Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry	Ooi et al.	2013	24367265	Cell line	U2OS	Sindbis virus (SINV) reporter	Luminescence	Ambion Silencer V3	Genome-wide	siRNA	Z-score	< -3 OR > 2
	5429	5429	POLH	np	28.4	none			76,9% viability
GR00313-A	5429	NM_006502	POLH	np	1.02	none				TNF-alpha pathway regulation	A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling.	Nickles et al.	2012	22733992	Cell line	HEK293T	NFkappaB pathway reporter	Luminescence	Qiagen	Genome-wide	siRNA	Z-score	<	Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
GR00318-A	5429	5429	POLH	np	0.632	Decreased caspase 3/7 activity			siRNA set: druggable genome	Huntingtin toxicity	A Genome-Scale RNA–Interference Screen Identifies RRAS Signaling as a Pathologic Feature of Huntington’s Disease	Miller et al.	2012	23209424	Cell line	HEK293T	Caspase 3/7 activity	Fluorescence	Dharmacon	Selected genes	siRNA	Sum of normalized caspase 3/7 activity mean and standard error	< 0.683	HEK293T cells were cotransfected with mutant Huntingtin fused to GFP (Htt1-558141Q-GFP).
GR00359-A	5429	5429	POLH	M-006454-00	-4.9075165314444416	Moderately decreased NFAT1-GFP nuclear translocation				NFAT activation	An siRNA screen for NFAT activation identifies septins as coordinators of store-operated Ca2+ entry	Sharma et al.	2013	23792561	Cell line	HeLa	NFAT1-GFP nuclear translocation	Fluorescence	Dharmacon siGENOME	Genome-wide	siRNA	Z-score	Strong: <	HeLa cells stably expressed NFAT1-GFP. Libraries were Dharmacon siGENOME v2007 and v2010
GR00360-A-1	5429	ENSG00000170734	POLH	119731	1.066	Strongly decreased CFP-tsO45G cell surface transport				Secretory transport (1)	Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway	Simpson et al.	2012	22660414	Cell line	HeLa Kyoto	CFP-tsO45G cell surface transport	Fluorescence	Ambion	Genome-wide	siRNA	Deviation score	< - 1 OR > 0.75 (mild: 0.75 - 1.0; strong: > 1.0)	For validation screen see Secretory transport (2).
GR00360-A-2	5429	ENSG00000170734	POLH	SI00089019	1.6218	Strongly decreased CFP-tsO45G cell surface transport				Secretory transport (2)	Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway	Simpson et al.	2012	22660414	Cell line	HeLa Kyoto	CFP-tsO45G cell surface transport	Fluorescence	Ambion and Qiagen	Selected genes	siRNA	Deviation score	> 1.0	For primary screen see Secretory transport (1). Several genes scored as hit but were not expressed in HeLa Kyoto cells, see comment. Qiagen custom: Reagent Sequences provided by authors.
GR00362-A	5429	5429	POLH	np	np	Synthetic lethal with vaccinia virus (VACV) infection				Vaccinia virus (VACV) replication	A loss of function analysis of host factors influencing Vaccina virus replication by RNA interference	Beard et al.	2014	2401222	Cell line	HeLa	VACV-A5eGFP expression	Fluorescence	SMARTpools, Dharmacon	Genome-wide	siRNA	Z-score	<	Genes were prescreened for viability, those declared as lethal were not screened for fluorescence.
GR00368-A	5429	5429	POLH		0.75	Decreased viability ratio			wt viability: 0,8	Combinatorial effect with E-Cadherin	Synthetic Lethal Screens Identify Vulnerabilities in GPCR Signaling and Cytoskeletal Organization in E-Cadherin–Deficient Cells	Telford et al.	2015	25777964	Cell line	MCF10A	Viability (synthetic lethal)	Luminescence	Dharmacon	Genome-wide	siRNA	CDH1(-/-)/wt viability ratio	<	MCF10A wild type viability higher-or-equal to 50% was considered as additional cutoff (values noted in "comment" section).
	5429	5429	POLH		-0.01999999999999999	none
GR00376-A-1	5429	5429	POLH		0.164077457	none				Mitigators of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2	5429	5429	POLH		0.015125149	none				Sensitizers of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
GR00378-A	5429		POLH		-0.198223832	none				Poliovirus vaccine production	Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game	van der Sanden et al.	2015	26581994	Cell line	HEp-2C	Infection with Attenuated Poliovirus	ELISA	Dharmacon	Genome-wide	siRNA	Z-score	>	For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol
	5429	NM_006502	POLH		25.542	none
GR00386-A-1	5429	5429	POLH		67.2190708081672	Decreased viability				NOD2 stimulation by MDP	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	Viability	Luminescence	Dharmacon	Genome-wide	siRNA	Percentage growth	Decreased: <70, increased: >120	Reagent IDs not provided
GR00386-A-2	5429	5429	POLH		-15.7	none				MDP-induced IL-8 secretion	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	IL-8 secretion	ELISA	Dharmacon	Genome-wide	siRNA	Percent inhibition of IL-8 secretion	Increased: <-300, Decreased: >60	Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided