RNAi

Gene Info

  • Species: Human (Homo sapiens)
  • GeneID: 79648
  • Symbol: MCPH1
  • Description: microcephalin 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/79648

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00018-A-0 79648 MCPH1 v2HS_69010 -1.01 (0.08) Synthetic lethal with Ras no Synthetic lethal interaction with Ras A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene. Luo et al. 2009 19490893 Cell line DLD-1 Synthetic lethal interaction with Ras Micoarray hybridization shRNA-mir (G. Hannon) shRNA Log2 diff MUT-WT (and P-value) -0.7 (0.3)
GR00053-A 79648 NM_024596 FLJ12847 np sp none no Genome stability A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. Paulsen et al. 2009 19647519 Cell line HeLa gamma-H2AX phosphorylation and DNA content Fluorescence siARRAY human genome siRNA library Genome-wide siRNA p-value Complex criteria Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A 79648 NM_024596 FLJ12847 np 0.996 none no Combinatorial effect with paclitaxel Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Whitehurst et al. 2007 17429401 Cell line NCI-H1155 Viability (synthetic lethal) ATP level # G-005000-01 Genome-wide siRNA Paclitaxel/control ratio Complex criteria Additional information about 87 high-confidence hits
GR00056-A 79648 NM_024596 FLJ12847 np 1.06 none no Melanogenesis Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. Ganesan et al. 2008 19057677 Cell line MNT-1 Melanin protein expression and viability Absorbance and luminescence rp Genome-wide siRNA Normalized absorbance ratio > 2 standard deviations below mean Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1 79648 NM_024596 FLJ12847 M-008447-01 0.36 none no Wnt/beta-catenin pathway regulation (1) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Z-score > 4 Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2 79648 NM_024596 FLJ12847 M-008447-01 sp none no Wnt/beta-catenin pathway regulation (2) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Complex, SP Complex criteria Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00095-A 79648 NM_024596 FLJ12847 np np none p53 pathway components A large-scale RNAi screen in human cells identifies new components of the p53 pathway Berns et al. 2004 15042092 Cell line BJ-TERT-tsLT fibroblasts Proliferation Colony-forming cell assay Nki library Genome-wide shRNA np np
GR00098-A-1 79648 ENSG00000147316 MCPH1 ENSG00000147316 sp none no Cell division (1) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell number and DNA content Laser scanning cytometry rp Genome-wide esiRNA Complex, sp Complex criteria
GR00123-A 79648 NM_024596 FLJ12847 137242, 137241, 137243 np none Combinatorial effect with nutlin-3 An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors Brummelkamp et al. 2006 16474381 Cell line MCF-7 Viability Fluoresence Nki library Genome-wide shRNA log2 ratio np
GR00151-A-1 79648 ENSG00000147316 MCPH1 np 0.969 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. SÅ‚abicki et al. 2010 20613862 Cell line HeLa (HR-DSBR) DR-GFP reporter Flow cytometry Custom-made Genome-wide esiRNA Z-score < -2 OR > 2
GR00180-A-1 79648 79648 MCPH1 PL-50022 0.92 none no Hepatitis C virus replication (1) A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. Tai et al. 2009 19286138 Cell line Huh7/Rep-Feo HCV replicon RNA copy number Luminescence siARRAY Human Genome siRNA Library Genome-wide siRNA q-value Complex criteria
GR00184-A-1 79648 NM_024596 MCPH1 M-008447-01 -1.92398723420089 none no Self-renewal and pluripotency in human embryonic stem cells (1) A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Chia et al. 2010 20953172 Cell line hESC H1 POU5F1 protein expression Fluorescence SMARTpool siRNA library Genome-wide siRNA Z-score < -2
GR00197-A-1 79648 79648 MCPH1 M-008447-01 -0.578155387 none no Human papillomavirus oncogene expression regulation (1) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Genome-wide siRNA Z-score > Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
79648 MCPH1 np np Decreased viability in colon lineage no colon: no filter, 2nd best
GR00236-A-1 79648 79648 MCPH1 M-008447-01 0.656901032292179 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Human siGENOME siRNA (G-005000-05) Genome-wide siRNA Relative HR ratio < ~0.4 OR > 1.88 Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1 79648 NM_024596 FLJ12847 M-008447-01 0.91 none yes TRAIL-induced apoptosis (1) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability Luminescence SMART-pool siRNA Genome-wide siRNA Z-score > 4 Author-submitted data
GR00240-S-2 79648 NM_024596 FLJ12847 M-008447-01 0.67 none no Z-score 1.855 TRAIL-induced apoptosis (2) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability (synthetic lethal) Luminescence SMART-pool siRNA Genome-wide siRNA Differential score > 3.6 AND viability Z-score < 4 Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1 79648 NM_024596 FLJ12847 np np none no Selective autophagy regulation (1) Image-based genome-wide siRNA screen identifies selective autophagy factors. Orvedahl et al. 2011 22020285 Cell line HeLa/GFP-LC3 Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression Fluorescence siGenome Genome-wide siRNA Z-score Complex criteria
GR00247-A-1 79648 MCPH1 np sp none rank: 1886 Regulation of FOXO1 nuclear localization (1) Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. Senapedis et al. 2011 21460183 Cell line U2OS EGFP-FOXO1a protein expression and DNA content Fluorescence Human Genome library Genome-wide siRNA Complex, sp Complex criteria
GR00249-S 79648 79648 FLJ12847 J-008447-06 -0.1394 none no number of cells compared to control (%): 86.68 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 79648 79648 FLJ12847 M-008447-02 -0.67761 none no number of cells compared to control (%): 80.80 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 79648 79648 MCPH1 s36004 -0.39363 none no number of cells compared to control (%): 88.43 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 79648 79648 MCPH1 s36005 -0.95809 none no number of cells compared to control (%): 71.05 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 79648 79648 MCPH1 s36006 1.53546 Increased vaccinia virus (VACV) infection no number of cells compared to control (%): 83.02 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00250-A-1 79648 79648 MCPH1 M-008447-01 sp Synthetic lethal with MLN4924 (a NAE inhibitor) duplicate screen Combinatorial effect with MLN4924, a NAE inhibitor (1) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line A375 Viability (synthetic lethal) Luminescence np Genome-wide siRNA Complex, sp Complex criteria Phenotypes for 650 nmol/L MLN4924. Additional information about screens with 250 nmol/L MLN4924.
GR00250-A-2 79648 79648 MCPH1 D-008447-02 sp Synthetic lethal with MLN4924 (a NAE inhibitor) sextuplicate screen Combinatorial effect with MLN4924, a NAE inhibitor (2) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line A375 Viability (synthetic lethal) Luminescence np Selected genes siRNA Complex, sp Complex criteria Phenotypes for 650 nmol/L MLN4924. Additional information about screens with 250 nmol/L MLN4924.
GR00250-A-2 79648 79648 MCPH1 D-008447-03 sp Synthetic lethal with MLN4924 (a NAE inhibitor) sextuplicate screen Combinatorial effect with MLN4924, a NAE inhibitor (2) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line A375 Viability (synthetic lethal) Luminescence np Selected genes siRNA Complex, sp Complex criteria Phenotypes for 650 nmol/L MLN4924. Additional information about screens with 250 nmol/L MLN4924.
GR00250-A-2 79648 79648 MCPH1 D-008447-04 sp Increased viability with MLN4924 (a NAE inhibitor) sextuplicate screen Combinatorial effect with MLN4924, a NAE inhibitor (2) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line A375 Viability (synthetic lethal) Luminescence np Selected genes siRNA Complex, sp Complex criteria Phenotypes for 650 nmol/L MLN4924. Additional information about screens with 250 nmol/L MLN4924.
GR00250-A-2 79648 79648 MCPH1 D-008447-05 sp none sextuplicate screen Combinatorial effect with MLN4924, a NAE inhibitor (2) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line A375 Viability (synthetic lethal) Luminescence np Selected genes siRNA Complex, sp Complex criteria Phenotypes for 650 nmol/L MLN4924. Additional information about screens with 250 nmol/L MLN4924.
GR00250-A-3 79648 79648 MCPH1 D-008447-02 sp none 91 nM MLN4924; set: 320DDR; final hit Combinatorial effect with MLN4924, a NAE inhibitor (3) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line HCT116 Viability (synthetic lethal) Luminescence np Selected and random genes siRNA Complex, sp Complex criteria
GR00250-A-3 79648 79648 MCPH1 D-008447-03 sp Synthetic lethal with MLN4924 (a NAE inhibitor) 91 nM MLN4924; set: 320DDR; final hit Combinatorial effect with MLN4924, a NAE inhibitor (3) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line HCT116 Viability (synthetic lethal) Luminescence np Selected and random genes siRNA Complex, sp Complex criteria
GR00250-A-3 79648 79648 MCPH1 D-008447-05 sp none 91 nM MLN4924; set: 320DDR; final hit Combinatorial effect with MLN4924, a NAE inhibitor (3) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line HCT116 Viability (synthetic lethal) Luminescence np Selected and random genes siRNA Complex, sp Complex criteria
GR00250-A-3 79648 79648 MCPH1 D-008447-18 sp none 91 nM MLN4924; set: 320DDR; final hit Combinatorial effect with MLN4924, a NAE inhibitor (3) Novel DNA damage checkpoints mediating cell death induced by the NEDD8-activating enzyme inhibitor MLN4924. Blank et al. 2013 23100467 Cell line HCT116 Viability (synthetic lethal) Luminescence np Selected and random genes siRNA Complex, sp Complex criteria
GR00253-A 79648 NM_024596 FLJ12847 np 1.054 none hepcidin regulation Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling. Mleczko-Sanecka et al. 2014 24385536 Cell line Huh7 hepcidin::fluc mRNA expression Luminescence siGenome siARRAY SMARTpool Genome-wide siRNA Z-score > Cutoff <
GR00255-A-1 79648 79648 MCPH1 TRCN0000083783, TRCN0000083784, TRCN0000083785, TRCN0000083786, TRCN0000083787 -0.450387055 none Negative genetic interactions (1) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.0 HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2 79648 79648 MCPH1 TRCN0000083783, TRCN0000083784, TRCN0000083785, TRCN0000083786, TRCN0000083787 -0.029672595 none Negative genetic interactions (2) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.0 HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3 79648 79648 MCPH1 TRCN0000083783, TRCN0000083784, TRCN0000083785, TRCN0000083786, TRCN0000083787 -0.217908735 none Negative genetic interactions (3) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.2 HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4 79648 79648 MCPH1 TRCN0000083783, TRCN0000083784, TRCN0000083785, TRCN0000083786, TRCN0000083787 -0.848435359 none Negative genetic interactions (4) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.2 HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5 79648 79648 MCPH1 TRCN0000083783, TRCN0000083784, TRCN0000083785, TRCN0000083786, TRCN0000083787 -0.137600248 none Negative genetic interactions (5) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -0.8 HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00257-A-1 79648 mcph1 127411 sp Effect on mitosis Cell division, migration and survival (1) Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann et al. 2010 20360735 Cell line HeLa H2B-GFP protein expression Fluorescence Mapped using ENSEMBL genome database version 27 Selected genes siRNA Complex, sp Complex criteria HeLa-H2B-GFP cells used.
GR00257-A-2 79648 mcph1 127411 sp Effect on mitosis Cell division, migration and survival (2) Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Neumann et al. 2010 20360735 Cell line HeLa H2B-GFP protein expression Fluorescence np Selected genes siRNA Complex, sp Complex criteria HeLa-H2B-GFP cells used.
GR00300-A 79648 MCPH1 TRCN0000083784, TRCN0000083783, TRCN0000083786, TRCN0000083785, TRCN0000083787 0 none Combinatorial effect with RAF inhibitor PLX4720 A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition. Whittaker et al. 2013 23288408 Cell line A375 shRNA abundance Sequencing TRC Genome-wide shRNA Number of shRNAs ranked Top1000 > 2 The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A 79648 NM_024596 FLJ12847 np -0.16 none Clear cell renal cell carcinoma (ccRCC) survival regulation Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target Gerlinger et al. 2012 22362593 Cell line VHL-deficient RCC4 Proliferation and Viability Fluorescence np Genome-wide siRNA Z-score < In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1 79648 79648 MCPH1 np -0.08 none Sindbis virus (SINV) infection (1) Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry Ooi et al. 2013 24367265 Cell line U2OS Sindbis virus (SINV) reporter Luminescence Ambion Silencer V3 Genome-wide siRNA Z-score < -3 OR > 2
79648 79648 MCPH1 np 53.1 none 82,4% viability
GR00313-A 79648 NM_024596 MCPH1 np -0.88 none TNF-alpha pathway regulation A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. Nickles et al. 2012 22733992 Cell line HEK293T NFkappaB pathway reporter Luminescence Qiagen Genome-wide siRNA Z-score < Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
GR00318-A 79648 79648 FLJ12847 np 1.169 none siRNA set: druggable genome Huntingtin toxicity A Genome-Scale RNA–Interference Screen Identifies RRAS Signaling as a Pathologic Feature of Huntington’s Disease Miller et al. 2012 23209424 Cell line HEK293T Caspase 3/7 activity Fluorescence Dharmacon Selected genes siRNA Sum of normalized caspase 3/7 activity mean and standard error < 0.683 HEK293T cells were cotransfected with mutant Huntingtin fused to GFP (Htt1-558141Q-GFP).
GR00327-A 79648 4180 TGDS CLL-H-025413 np none Tumor formation An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation Wolf et al. 2013 24292671 Cell line SUM-149 shRNA abundance Next-generation sequencing Decipher library module 1 Selected genes shRNA Z-score > 2.24 Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A 79648 4180 TGDS CLL-H-025415 -0.34478578896258283 none Tumor formation An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation Wolf et al. 2013 24292671 Cell line SUM-149 shRNA abundance Next-generation sequencing Decipher library module 1 Selected genes shRNA Z-score > 2.24 Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A 79648 4180 TGDS CLL-H-025411 np none Tumor formation An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation Wolf et al. 2013 24292671 Cell line SUM-149 shRNA abundance Next-generation sequencing Decipher library module 1 Selected genes shRNA Z-score > 2.24 Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A 79648 4180 TGDS CLL-H-025414 0.9454249085941444 none Tumor formation An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation Wolf et al. 2013 24292671 Cell line SUM-149 shRNA abundance Next-generation sequencing Decipher library module 1 Selected genes shRNA Z-score > 2.24 Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
GR00327-A 79648 4180 TGDS CLL-H-025412 1.1890100749712098 none Tumor formation An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation Wolf et al. 2013 24292671 Cell line SUM-149 shRNA abundance Next-generation sequencing Decipher library module 1 Selected genes shRNA Z-score > 2.24 Genes were scored as hit if at least 2 shRNAs scored as hit, see comment.
79648 MCPH1 np 1 Resistant to vaccinia virus (VACV-A4L) infection
79648 79648 MCPH1 -0.04000000000000001 none
GR00376-A-1 79648 79648 MCPH1 0.013834463 none Mitigators of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2 79648 79648 MCPH1 0.128655369 none Sensitizers of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
79648 NM_024596 MCPH1 23.82 Decreased viability
GR00386-A-1 79648 79648 MCPH1 92.6359829633324 none NOD2 stimulation by MDP A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 Viability Luminescence Dharmacon Genome-wide siRNA Percentage growth Decreased: <70, increased: >120 Reagent IDs not provided
GR00386-A-2 79648 79648 MCPH1 -0.5 none MDP-induced IL-8 secretion A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 IL-8 secretion ELISA Dharmacon Genome-wide siRNA Percent inhibition of IL-8 secretion Increased: <-300, Decreased: >60 Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided