RNAi

Gene Info

  • Species: Human (Homo sapiens)
  • GeneID: 84876
  • Symbol: ORAI1
  • Description: ORAI calcium release-activated calcium modulator 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/84876

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00053-A 84876 NM_032790 FLJ14466 np sp none no Genome stability A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. Paulsen et al. 2009 19647519 Cell line HeLa gamma-H2AX phosphorylation and DNA content Fluorescence siARRAY human genome siRNA library Genome-wide siRNA p-value Complex criteria Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A 84876 NM_032790 FLJ14466 np 0.867 none no Combinatorial effect with paclitaxel Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Whitehurst et al. 2007 17429401 Cell line NCI-H1155 Viability (synthetic lethal) ATP level # G-005000-01 Genome-wide siRNA Paclitaxel/control ratio Complex criteria Additional information about 87 high-confidence hits
GR00056-A 84876 NM_032790 FLJ14466 np 1.137 none no Melanogenesis Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. Ganesan et al. 2008 19057677 Cell line MNT-1 Melanin protein expression and viability Absorbance and luminescence rp Genome-wide siRNA Normalized absorbance ratio > 2 standard deviations below mean Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1 84876 NM_032790 FLJ14466 M-014998-00 -0.7 none no Wnt/beta-catenin pathway regulation (1) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Z-score > 4 Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2 84876 NM_032790 FLJ14466 M-014998-00 sp none no Wnt/beta-catenin pathway regulation (2) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Complex, SP Complex criteria Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00098-A-1 84876 ENSG00000182500 TMEM142A ENSG00000182500 sp none no Cell division (1) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell number and DNA content Laser scanning cytometry rp Genome-wide esiRNA Complex, sp Complex criteria
GR00151-A-1 84876 ENSG00000182500 ORAI1 np -0.457 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. SÅ‚abicki et al. 2010 20613862 Cell line HeLa (HR-DSBR) DR-GFP reporter Flow cytometry Custom-made Genome-wide esiRNA Z-score < -2 OR > 2
GR00180-A-1 84876 84876 TMEM142A PL-50068 0.828 none no Hepatitis C virus replication (1) A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. Tai et al. 2009 19286138 Cell line Huh7/Rep-Feo HCV replicon RNA copy number Luminescence siARRAY Human Genome siRNA Library Genome-wide siRNA q-value Complex criteria
GR00184-A-1 84876 NM_032790 ORAI1 M-014998-00 -1.81765328635136 none no Self-renewal and pluripotency in human embryonic stem cells (1) A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Chia et al. 2010 20953172 Cell line hESC H1 POU5F1 protein expression Fluorescence SMARTpool siRNA library Genome-wide siRNA Z-score < -2
GR00197-A-1 84876 84876 TMEM142A M-014998-00 1.2951231 none no Human papillomavirus oncogene expression regulation (1) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Genome-wide siRNA Z-score > Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00233-A-1 84876 84876 TMEM142A np np Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection yes siRNA pool validated Hepatitis C virus (HCV) infection (1) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after virus infection Fluorescence Human Genome siARRAY siRNA G-005000–05 Genome-wide siRNA Percentage of infected cells <
GR00233-A-2 84876 84876 TMEM142A D-014998-04 0.19 Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection no Hepatitis C virus (HCV) infection (2) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after direct virus infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-2 84876 84876 TMEM142A D-014998-02 0.72 none no Hepatitis C virus (HCV) infection (2) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after direct virus infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-2 84876 84876 TMEM142A D-014998-03 0.04 Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection no Hepatitis C virus (HCV) infection (2) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after direct virus infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-2 84876 84876 TMEM142A D-014998-01 0.56 none no Hepatitis C virus (HCV) infection (2) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after direct virus infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-3 84876 84876 TMEM142A D-014998-04 0.39 Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection no Hepatitis C virus (HCV) infection (3) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after viral supernatant infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-3 84876 84876 TMEM142A D-014998-02 0.35 Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection no Hepatitis C virus (HCV) infection (3) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after viral supernatant infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-3 84876 84876 TMEM142A D-014998-03 0.27 Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection no Hepatitis C virus (HCV) infection (3) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after viral supernatant infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00233-A-3 84876 84876 TMEM142A D-014998-01 0.85 none no Hepatitis C virus (HCV) infection (3) A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Li et al. 2009 19717417 Cell line Huh7.5.1 HCV core protein expression and DNA content after viral supernatant infection Fluorescence siARRAY siRNA Selected genes siRNA Normalized percentage of infected cells <
GR00236-A-1 84876 84876 TMEM142A M-014998-00 1.16628226331355 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Human siGENOME siRNA (G-005000-05) Genome-wide siRNA Relative HR ratio < ~0.4 OR > 1.88 Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1 84876 NM_032790 FLJ14466 M-014998-00 0.69 none yes TRAIL-induced apoptosis (1) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability Luminescence SMART-pool siRNA Genome-wide siRNA Z-score > 4 Author-submitted data
GR00240-S-2 84876 NM_032790 FLJ14466 M-014998-00 -1.08 none no Z-score -0.4155 TRAIL-induced apoptosis (2) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability (synthetic lethal) Luminescence SMART-pool siRNA Genome-wide siRNA Differential score > 3.6 AND viability Z-score < 4 Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1 84876 NM_032790 FLJ14466 np sp none no Selective autophagy regulation (1) Image-based genome-wide siRNA screen identifies selective autophagy factors. Orvedahl et al. 2011 22020285 Cell line HeLa/GFP-LC3 Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression Fluorescence siGenome Genome-wide siRNA Z-score Complex criteria
GR00247-A-1 84876 TMEM142A np sp none rank: 12438 Regulation of FOXO1 nuclear localization (1) Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. Senapedis et al. 2011 21460183 Cell line U2OS EGFP-FOXO1a protein expression and DNA content Fluorescence Human Genome library Genome-wide siRNA Complex, sp Complex criteria
GR00249-S 84876 84876 FLJ14466 J-014998-05 -0.60042 none no number of cells compared to control (%): 84.63 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 84876 84876 FLJ14466 M-014998-01 -0.1261 none no number of cells compared to control (%): 56.58 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 84876 84876 TMEM142A s228396 0.52109 none no number of cells compared to control (%): 99.56 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 84876 84876 ORAI1 s39558 1.89456 Increased vaccinia virus (VACV) infection no number of cells compared to control (%): 96.39 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 84876 84876 ORAI1 s39560 0.38471 none no number of cells compared to control (%): 102.03 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00253-A 84876 NM_032790 FLJ14466 np -1.281 none hepcidin regulation Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling. Mleczko-Sanecka et al. 2014 24385536 Cell line Huh7 hepcidin::fluc mRNA expression Luminescence siGenome siARRAY SMARTpool Genome-wide siRNA Z-score > Cutoff <
GR00297-A 84876 ORAI1 TRCN0000158974 -0.21 none Glucose limitation response Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides Birsoy et al. 2014 24670634 Cell line Jurkat shRNA abundance in high and low glucose Deep sequencing TRC Selected genes shRNA Log2 ratio < -0.75
GR00297-A 84876 ORAI1 TRCN0000166446 -0.12 none Glucose limitation response Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides Birsoy et al. 2014 24670634 Cell line Jurkat shRNA abundance in high and low glucose Deep sequencing TRC Selected genes shRNA Log2 ratio < -0.75
GR00297-A 84876 ORAI1 TRCN0000159378 0.11 none Glucose limitation response Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides Birsoy et al. 2014 24670634 Cell line Jurkat shRNA abundance in high and low glucose Deep sequencing TRC Selected genes shRNA Log2 ratio < -0.75
GR00297-A 84876 ORAI1 TRCN0000165112 0.31 none Glucose limitation response Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides Birsoy et al. 2014 24670634 Cell line Jurkat shRNA abundance in high and low glucose Deep sequencing TRC Selected genes shRNA Log2 ratio < -0.75
GR00297-A 84876 ORAI1 TRCN0000163585 0.02 none Glucose limitation response Metabolic determinants of cancer cell sensitivity to glucose limitation and biguanides Birsoy et al. 2014 24670634 Cell line Jurkat shRNA abundance in high and low glucose Deep sequencing TRC Selected genes shRNA Log2 ratio < -0.75
GR00303-A 84876 NM_032790 FLJ14466 np -0.24 none Clear cell renal cell carcinoma (ccRCC) survival regulation Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target Gerlinger et al. 2012 22362593 Cell line VHL-deficient RCC4 Proliferation and Viability Fluorescence np Genome-wide siRNA Z-score < In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1 84876 84876 FLJ14466 np -0.04 none Sindbis virus (SINV) infection (1) Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry Ooi et al. 2013 24367265 Cell line U2OS Sindbis virus (SINV) reporter Luminescence Ambion Silencer V3 Genome-wide siRNA Z-score < -3 OR > 2
84876 84876 ORAI1 np 55.3 none 88,4% viability
GR00313-A 84876 NM_032790 FLJ14466 np 0.66 none TNF-alpha pathway regulation A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. Nickles et al. 2012 22733992 Cell line HEK293T NFkappaB pathway reporter Luminescence Qiagen Genome-wide siRNA Z-score < Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
GR00359-A 84876 84876 TMEM142A M-014998-00 -4.3426857484914425 Moderately decreased NFAT1-GFP nuclear translocation NFAT activation An siRNA screen for NFAT activation identifies septins as coordinators of store-operated Ca2+ entry Sharma et al. 2013 23792561 Cell line HeLa NFAT1-GFP nuclear translocation Fluorescence Dharmacon siGENOME Genome-wide siRNA Z-score Strong: < HeLa cells stably expressed NFAT1-GFP. Libraries were Dharmacon siGENOME v2007 and v2010
GR00360-A-1 84876 ENSG00000182500 ORAI1 216907 0.8148249999999999 Mildly decreased CFP-tsO45G cell surface transport Secretory transport (1) Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway Simpson et al. 2012 22660414 Cell line HeLa Kyoto CFP-tsO45G cell surface transport Fluorescence Ambion Genome-wide siRNA Deviation score < - 1 OR > 0.75 (mild: 0.75 - 1.0; strong: > 1.0) For validation screen see Secretory transport (2).
GR00376-A-1 84876 84876 ORAI1 0.315988268 none Mitigators of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2 84876 84876 ORAI1 0.2507523 none Sensitizers of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
84876 NM_032790 ORAI1 33.985 none
GR00386-A-1 84876 84876 ORAI1 88.6027485554253 none NOD2 stimulation by MDP A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 Viability Luminescence Dharmacon Genome-wide siRNA Percentage growth Decreased: <70, increased: >120 Reagent IDs not provided
GR00386-A-2 84876 84876 ORAI1 57.6 none MDP-induced IL-8 secretion A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. Warner et al. 2014 25170077 Cell line HEK293 stably expressing NOD2 IL-8 secretion ELISA Dharmacon Genome-wide siRNA Percent inhibition of IL-8 secretion Increased: <-300, Decreased: >60 Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided