| 19055 |
P63328 |
PP2BA_MOUSE |
Protein phosphatase 3 catalytic subunit alpha (EC 3.1.3.16) (CAM-PRP catalytic subunit) (Calcineurin A alpha) (Calmodulin-dependent calcineurin A subunit alpha isoform) (CNA alpha) (Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform) |
DISRUPTION PHENOTYPE |
Knockout mice are significantly smaller at postnatal day 18 (P18), including significantly reduced weights of the liver and kidneys (PubMed:15509543, PubMed:16735444). Decreased blood glucose levels (PubMed:16735444). Decreased lumber spine, tibia, and total body bone mass density evident at 6 weeks of age, evidence of decreased density in the lumber spine as early as 3 weeks of age (PubMed:16286645). No change in overall muscle weight (PubMed:12773574). Decreased femur length, reduced cortical trabecular bone thickness (PubMed:16286645). Significantly reduced number of differentiated osteoclasts (PubMed:16968888). Reduced mitochondrial oxidative capacity in slow and intermediate muscle fiber types (PubMed:12773574). Reduced slow and intermediate program type muscle fibers in the biceps and triceps (PubMed:12773574). Decreased number of slow program type muscle fibers and NFAT activity in the soleus (PubMed:12773574). Kidney size and development is normal at P4, however by P18 kidneys show an obvious delay in maturation, displaying a decreased overall mass, poorly defined medullary rays and decreased cortical mass (PubMed:15509543). Upon examination the outer strip of the medulla and cortical regions of the kidneys are significantly decreased (PubMed:15509543). In the cortex there is a persistence of poorly developed surface glomeruli due to attenuation of mesangial cells numbers and a lack of maturation of tubules in the nephrogenic zone (PubMed:15509543). Reduced proliferation and increased apoptosis of cells within the nephrogenic zone at P18, potentially as a result of increased p27 expression (PubMed:15509543). Impaired kidney function evident by increased kidney collagen deposition, serum creatinine levels and decreased urine creatinine concentration from P4 onwards (PubMed:15509543, PubMed:16735444). Loss of AQP2 phosphorylation in response to vasopressin and decreased localization to the apical membrane of inner medullary collecting duct cells (PubMed:16735444). Most mice die between P21 and P28 as a result of progressive kidney failure (PubMed:15509543). Increased salivary osmolality despite normal electrolyte composition and protein content (PubMed:21435446). Decreased activity of amylase, peroxidase, lysozyme and sialic acid in the saliva (PubMed:21435446). Decreased number of secretory vesicles, mucosal acini cell size and protein content of serosal acini in the submandibular glands (PubMed:21435446). Decreased activity of calcineurin in the salivary glands (PubMed:21435446). Decreased thickness of the epidermal stratum spinosum, a thickened corneum and increased sloughing-off of keratinocytes in newborn mice (PubMed:19626032). Decreased thickness of the stratum spinosum is still evident at 4 weeks of age along with decreased skin elasticity (PubMed:19626032). Increased apoptosis in the supra-basal layers and the stratum spinosum of the epidermis (PubMed:19626032). Decrease in NFATc activity in basal epidermal cells and impaired differentiation of epidermal keratinocytes as shown by aberrant expression of the differentiation markers KRT14, KRT10 and IVL (PubMed:19626032). Decreased calcineurin activity in the brain and significant reduction in homosynaptic depotentiation (PubMed:10200317). Decreased calcineurin activity in T-lymphocytes and loss of T-lymphocyte proliferation in response to antigen stimulation (PubMed:8627154). {ECO:0000269|PubMed:10200317, ECO:0000269|PubMed:12773574, ECO:0000269|PubMed:15509543, ECO:0000269|PubMed:16286645, ECO:0000269|PubMed:16735444, ECO:0000269|PubMed:16968888, ECO:0000269|PubMed:19626032, ECO:0000269|PubMed:21435446, ECO:0000269|PubMed:8627154}. |