| 173783 |
Q23194 |
VPS18_CAEEL |
Vacuolar protein sorting-associated protein 18 homolog |
DISRUPTION PHENOTYPE |
Temperature-sensitive defects in the formation of gut granules during embryogenesis (PubMed:24501423). At 15 degrees Celsius, pretzel-stage embryos have a reduced number of gut granules in intestinal cells, and at 22 degrees Celsius, pretzel-stage embryos completely lack gut granules in intestinal cells (PubMed:24501423). At 25 degrees Celsius, many embryos arrest by the pre-bean stage before elongation, and 76% of these embryos contain gut granules irregularly distributed throughout the embryo (PubMed:24501423). Defective apoptotic germ cell corpse digestion with delayed degradation of chromatin in late germ cell corpses (PubMed:18923146). This results in increased numbers of germ cell corpses at 20 degrees Celsius during embryogenesis and post the L4 stage of larval development, and furthermore the retention of cell corpses for a longer duration of time (PubMed:18923146). Impaired formation of endosomes and lysosomes in coelomocytes, in particular there is impaired formation of recycling endosomes (PubMed:18923146). In addition, there are endosome/lysosome fusion defects in coelomocytes (PubMed:18923146, PubMed:26783301). RNAi-mediated knockdown results in defective endosome maturation with the accumulation of small vesicles near the gut lumen and large endosomes/lysosomes on the basal side of the cell (PubMed:25273556). Double knockout with either sorf-1 or sorf-2, results in larger endosomes and larger lysosomes and thus suppresses the endosome/lysosome fusion defect in the vps-18 single mutant (PubMed:26783301). {ECO:0000269|PubMed:18923146, ECO:0000269|PubMed:24501423, ECO:0000269|PubMed:25273556, ECO:0000269|PubMed:26783301}. |