| 174095 |
Q18891 |
VP33B_CAEEL |
Vacuolar protein sorting-associated protein 33B |
DISRUPTION PHENOTYPE |
Hermaphrodites exhibit sperm-specific sterility and only lay unfertilized eggs (PubMed:27558849). In contrast to wild-type animals, mutant males produce no or few spermatids in their gonads, but instead their gonads accumulate terminally arrested spermatocytes that do not bud and contain four haploid nuclei (PubMed:27558849). In some instances, the spermatocytes have small buds that do not form spermatids (PubMed:27558849). Oocytes and unfertilized eggs contain some larger endocytic structures tethered to yolk granules (PubMed:27558849). RNAi-mediated knockdown results in defective yolk uptake in oocytes with the appearance of endocytic structures containing yolk tethered to large membranous organelles which could be lysosomes (PubMed:25273556). RNAi-mediated knockdown results in defective endosome maturation with the accumulation of small vesicles near the gut lumen which is possibly indicative of increased endosomal fusion activity, and defective trafficking of proteins such as lmp-1 to lysosomal compartments (PubMed:25273556). RNAi-mediated knockdown in a sand-1 mutant background results in defective endosome fusion with the formation of larger, irregularly-shaped rab-5 positive endosomes in oocytes and intestinal cells, and larger yolk-containing granules than in the sand-1 single mutant (PubMed:25273556). Double RNAi-mediated knockdown together with vsp-33.1 results in defective protein trafficking to lysosomal compartments, and an irregular distribution of vesicles of various sizes throughout the gut cells (PubMed:25273556). Double RNAi-mediated knockdown together with vsp-33.1 in a sand-1 mutant background rescues the large endosome phenotype and the defective protein trafficking to lysosomal compartments in the sand-1 single mutant, but results in lethality (PubMed:25273556). {ECO:0000269|PubMed:25273556, ECO:0000269|PubMed:27558849}. |