| 174779 |
Q19131 |
NPP5_CAEEL |
Nuclear pore complex protein 5 (Nuclear pore complex protein Nup107) (Nucleoporin npp-5) |
DISRUPTION PHENOTYPE |
Larval lethality with about 9-12 percent of animals reaching adulthood. The few surviving adults are sterile. About 10 percent embryonic lethality. Reduced nuclear growth in P1 embryonic cell and delayed entry into mitosis. 39 percent reduction in him-10 kinetochore association and 34 percent reduction in air-2 chromatin association during metaphase. Loss of npp-5 localization to kinetochores during metaphase. About 10 percent of mutants have chromosome segregation defects. RNAi-mediated knockdown causes a loss of npp-23 localization to kinetochores and reduced localization to the nuclear envelope in interphase. Also results in the accumulation of spindle assembly checkpoint protein mdf-1 on kinetochores during metaphase and reduced mdf-1 localization to the nuclear envelope. Does not affect the expression levels of nuclear pore complex components npp-10, npp-19 and npp-7 or npp-10, npp-2 and mel-28 localization and appears to be dispensable for pie-1 nuclear import and for nuclear protein exclusion. Embryos transiently exposed to a hypoxic environment develop into infertile adults. Simultaneous RNAi-mediated knockdown of mdf-1 causes embryonic lethality and severe chromosome segregation defects. {ECO:0000269|PubMed:22238360}. |