| 175133 |
G5ECG0 |
TACC1_CAEEL |
Transforming acid coiled-coil-containing protein 1 |
DISRUPTION PHENOTYPE |
Maternal-effect embryonic lethal (PubMed:23155404). RNAi-mediated knockdown results in a meiotic defect in 30% of early embryos and defective microtubule related processes during the first cell cycle in early embryogenesis (PubMed:12956950, PubMed:12956951, PubMed:12956952). In one-cell embryos, the maternal pronuclei fail to migrate and the breakdown of the pronuclear envelope is delayed (PubMed:12956950, PubMed:12956951, PubMed:12956952). The sperm pronuclear complex also exhibits a migratory defect whereby it does not move to the center of the embryo or rotate on the anterior-posterior axis leading to the assembly of a misaligned spindle at the posterior of the embryo and ingression of the cleavage furrow from the posterior cortex (PubMed:12956950, PubMed:12956951, PubMed:12956952, PubMed:17666432). Reduced microtubule growth rate in embryos (PubMed:16054029). Defective microtubule elongation in one-cell embryos resulting in short microtubules that extend from the centrosome, but do not extend to the cortex, and mitotic spindles that are 20% shorter than in wild-type one-cell embryos (PubMed:12956950, PubMed:12956951). Specifically, one-cell embryos have shorter astral and mitotic spindle microtubules (PubMed:12956952). Reduced stability of the zyg-9/tac-1 complex with reduced accumulation of zyg-9 at the centrosomes (PubMed:12956950). {ECO:0000269|PubMed:12956950, ECO:0000269|PubMed:12956951, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:16054029, ECO:0000269|PubMed:17666432}. |