| 176221 |
P34260 |
VP33A_CAEEL |
Vacuolar protein sorting-associated protein 33A (Protein slp-1) |
DISRUPTION PHENOTYPE |
Viable, and develop into fertile adults, but their progeny arrest before embryonic morphogenesis (PubMed:27558849). Defective endosome/lysosome size and fusion, and delayed endocytic trafficking to lysosomes in coelomocytes (PubMed:26783301, PubMed:27558849). Reduced number of gut granules in the adult intestine (PubMed:24501423, PubMed:27558849). RNAi-mediated knockdown results in a reduced number of gut granules in embryonic intestinal cells (PubMed:24501423). RNAi-mediated knockdown results in the accumulation of lysosomal proteins such as lmp-1 in early endocytic compartments, and the formation of large late endosomes/lysosomes, but with simultaneous expression of rab-5- and rab-7-positive vesicles on the basal side of gut cells (PubMed:25273556). Double RNAi-mediated knockdown together with vsp-33.2 results in defective protein trafficking to lysosomal compartments, and an irregular distribution of vesicles of various sizes throughout the gut cells. RNAi-mediated knockdown in a sand-1 mutant background results in no viable offspring (PubMed:25273556). Double RNAi-mediated knockdown together with vsp-33.1 in a sand-1 mutant background rescues the large endosome phenotype and the defective protein trafficking to lysosomal compartments in the sand-1 single mutant, but still results in lethality (PubMed:25273556). Double knockout with tbc-2 or single knockout in a constitutively active rab-5 mutant background, rescues the large endosome formation defect in coelomocytes in the individual tbc-2 single and constitutively active rab-5 mutants (PubMed:27558849). {ECO:0000269|PubMed:24501423, ECO:0000269|PubMed:25273556, ECO:0000269|PubMed:26783301, ECO:0000269|PubMed:27558849}. |