| 34544 |
Q9VKJ8 |
IDUA_DROME |
Alpha-L-iduronidase (EC 3.2.1.76) (D-idua) |
DISRUPTION PHENOTYPE |
RNAi-mediated knockdown results in dysregulated glycolysis and lipogenesis, and is pupal lethal (PubMed:35011691). RNAi-mediated knockdown causes the incorrect acidification of lysosomes leading to dysfunctional lysosome-autophagosome fusion and consequently, blocks autophagy flux (PubMed:35011691). RNAi-mediated knockdown results in a significant increase in the number and size of lysosomes in the brain (PubMed:35011691). Expression levels of genes involved in glycolysis (Pfk, Tpi and Ldh) and lipogenesis (ACC) are increased (PubMed:35011691). Conditional RNAi-mediated knockdown causes developmental defects leading to premature death at different stages, depending on the tissue and on the extent of the expression reduction (PubMed:35011691). RNAi-mediated knockdown in muscles results in complete lethality at the pupal stage, knockdown in glial cells results in partial lethality at the pupal stage (about 73% of flies reach the adult stage), whereas knockdown in neurons is not lethal (PubMed:35011691). Conditional RNAi-mediated knockdown in the muscles results in a significant increase in the number and size of lysosomes in the muscles (PubMed:35011691). RNAi-mediated knockdown in glial cells and also in neurons, causes an age-dependent climbing decline and increases adult lifespan (PubMed:35011691). {ECO:0000269|PubMed:35011691}. |