| 36733 |
Q7JVA5 |
CEP89_DROME |
Protein Cep89 homolog |
DISRUPTION PHENOTYPE |
RNAi-mediated knockdown of the protein causes complete lethality at a late pupal stage. Muscle-specific knockdown is lethal under strongly induced conditions, but shows no obvious alteration in locomotion and behavior under mild induction conditions. Neuron-specific knockdown flies show striking motor defects. Analysis of synaptic and subsynaptic organization at larval neuromuscular junction shows normal overall synapse morphology, but a highly significant decrease in the amount of active zones, i.e. presynaptic microdomains of neurotransmitter release. Muscle size is smaller in knockdown animals compared to wild-type. Neuron-specific knockdown flies show decreased ability for non-associative learning, tested by non-associative jump-reflex habituation. Wing-specific knockdown produces flies with a dramatically reduced wing size. Eye-specific knockdown disturbs ommatidia and bristle organization and corrupts integrity of mitochondria. Electroretinograms show a gross defect in basal neurotransmission. {ECO:0000269|PubMed:23575228}. |