Mine Disruption Phenotype

Gene Info

  • Species: Zebrafish (Danio rerio)
  • GeneID: 559080
  • Symbol: cftr
  • Description: CF transmembrane conductance regulator
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Gene ID Entry Entry Name Protein Name Type Information
559080 Q1LX78 CFTR_DANRE Cystic fibrosis transmembrane conductance regulator (EC 5.6.1.6) (ATP-binding cassette sub-family C member 7) (Channel conductance-controlling ATPase) (cAMP-dependent chloride channel) DISRUPTION PHENOTYPE Considerable lethality around 10 dpf (PubMed:25592226). No effect on initial pancreas development, but at 16 dpf mutants display loss of pancreatic acinar tissue (PubMed:25592226). At 22 dpf, most pancreatic acinar tissue has disappeared and has been replaced by fibrotic tissue that surrounds dilated, mucus-filled ducts (PubMed:25592226). Morpholino knockdown of the protein in 48 hpf embryos leads to impaired resistance to P.aeruginosa strain PA14 and strain SMC573, as shown by the increased bacterial burden, but there is no effect on resistance to E.tarda, B.cenocepacia, S.aureus MZ100, E.coli XL-10 and H.influenzae Hib EAGAN (PubMed:20732993). Morpholino knockdown of the protein causes an important reduction of the volume of Kupffer's vesicle during embryonic development (PubMed:26432887). {ECO:0000269|PubMed:20732993, ECO:0000269|PubMed:25592226, ECO:0000269|PubMed:26432887}.