Crispr

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 207
• Symbol: AKT1
• Description: AKT serine/threonine kinase 1

DataSource: http://genomecrispr.dkfz.de/#!/results/AKT1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
104776682	104776705	14	+	26472758	Jiyoye	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.3047072246478	[597]	[556]	2	hSpCas9	negative selection	5046
104775720	104775743	14	-	26472758	Jiyoye	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	5.451569500864326	[0]	[32]	9	hSpCas9	negative selection	5047
104775132	104775155	14	-	26472758	Jiyoye	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	1.2467107093126273	[37]	[67]	7	hSpCas9	negative selection	5048
104770819	104770842	14	+	26472758	Jiyoye	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-4.680287459744466	[203]	[5]	-9	hSpCas9	negative selection	5049
104780194	104780217	14	-	26472758	Jiyoye	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.5956204709189837	[128]	[146]	4	hSpCas9	negative selection	5050
104780127	104780150	14	-	26472758	Jiyoye	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.08317295838065253	[173]	[138]	0	hSpCas9	negative selection	5051
104780115	104780138	14	-	26472758	Jiyoye	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.13339299985682934	[175]	[120]	-1	hSpCas9	negative selection	5052
104776722	104776745	14	+	26472758	Jiyoye	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	-0.6569549559138421	[22]	[10]	-5	hSpCas9	negative selection	5053
104775768	104775791	14	-	26472758	Jiyoye	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.09163047546556835	[64]	[45]	0	hSpCas9	negative selection	5054
104775744	104775767	14	-	26472758	Jiyoye	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.1650085144939799	[280]	[188]	-1	hSpCas9	negative selection	5055
104776682	104776705	14	+	26472758	KBM7	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.05540909284878026	[3366,2988]	[1779,1345]	0	hSpCas9	negative selection	196164
104775720	104775743	14	-	26472758	KBM7	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-0.12277594737782382	[176,107]	[44,73]	-1	hSpCas9	negative selection	196165
104775132	104775155	14	-	26472758	KBM7	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.0017503373102184527	[376,356]	[215,133]	0	hSpCas9	negative selection	196166
104770819	104770842	14	+	26472758	KBM7	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-0.36099356795489085	[369,366]	[191,84]	-3	hSpCas9	negative selection	196167
104780194	104780217	14	-	26472758	KBM7	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.5018499928842082	[827,600]	[261,209]	-4	hSpCas9	negative selection	196168
104780127	104780150	14	-	26472758	KBM7	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.2672209594483348	[762,544]	[310,199]	-3	hSpCas9	negative selection	196169
104780115	104780138	14	-	26472758	KBM7	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.20291225187876638	[667,847]	[318,504]	2	hSpCas9	negative selection	196170
104776722	104776745	14	+	26472758	KBM7	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	2.1867031094660465	[41,53]	[8,189]	9	hSpCas9	negative selection	196171
104775768	104775791	14	-	26472758	KBM7	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.34376532995276876	[219,209]	[135,121]	3	hSpCas9	negative selection	196172
104775744	104775767	14	-	26472758	KBM7	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.22576999508434925	[881,1093]	[450,641]	2	hSpCas9	negative selection	196173
104776682	104776705	14	+	26472758	Raji	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.5110376070248546	[1264]	[205]	-4	hSpCas9	negative selection	387282
104775720	104775743	14	-	26472758	Raji	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-1.9800793058767399	[135]	[7]	-8	hSpCas9	negative selection	387283
104775132	104775155	14	-	26472758	Raji	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.587496657425592	[258]	[39]	-5	hSpCas9	negative selection	387284
104770819	104770842	14	+	26472758	Raji	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.7255064787911423	[227]	[15]	-8	hSpCas9	negative selection	387285
104780194	104780217	14	-	26472758	Raji	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.6689444910633803	[321]	[46]	-5	hSpCas9	negative selection	387286
104780127	104780150	14	-	26472758	Raji	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.7385162175276816	[327]	[126]	7	hSpCas9	negative selection	387287
104780115	104780138	14	-	26472758	Raji	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.32121394320662344	[337]	[97]	3	hSpCas9	negative selection	387288
104776722	104776745	14	+	26472758	Raji	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	-0.2551865440111092	[35]	[6]	-2	hSpCas9	negative selection	387289
104775768	104775791	14	-	26472758	Raji	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.37041794120739324	[109]	[32]	3	hSpCas9	negative selection	387290
104775744	104775767	14	-	26472758	Raji	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.3883116272504694	[422]	[74]	-3	hSpCas9	negative selection	387291
104776682	104776705	14	+	26472758	K562	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.05175500388592358	[2192]	[1785]	0	hSpCas9	negative selection	578400
104775720	104775743	14	-	26472758	K562	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-1.3185174667247104	[129]	[43]	-6	hSpCas9	negative selection	578401
104775132	104775155	14	-	26472758	K562	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-1.0521125901554338	[365]	[148]	-5	hSpCas9	negative selection	578402
104770819	104770842	14	+	26472758	K562	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-3.4494781446078733	[439]	[33]	-8	hSpCas9	negative selection	578403
104780194	104780217	14	-	26472758	K562	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.16955080688394908	[612]	[581]	1	hSpCas9	negative selection	578404
104780127	104780150	14	-	26472758	K562	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	1.0029292484314962	[544]	[921]	8	hSpCas9	negative selection	578405
104780115	104780138	14	-	26472758	K562	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.710736731888984	[607]	[839]	6	hSpCas9	negative selection	578406
104776722	104776745	14	+	26472758	K562	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	1.4711895895134692	[46]	[109]	9	hSpCas9	negative selection	578407
104775768	104775791	14	-	26472758	K562	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.727360291447094	[165]	[231]	6	hSpCas9	negative selection	578408
104775744	104775767	14	-	26472758	K562	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.3075861985047783	[781]	[816]	2	hSpCas9	negative selection	578409
104774926	104774949	14	+	26627737	DLD1	viability	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	0.5004557973430033	[102]	[68,11,52]	5	hSpCas9	negative selection	787702
104775153	104775176	14	-	26627737	DLD1	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.05856311548055071	[687]	[119,130,343]	0	hSpCas9	negative selection	787703
104775705	104775728	14	+	26627737	DLD1	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	-0.1340377904192639	[920]	[186,302,293]	-1	hSpCas9	negative selection	787704
104776682	104776705	14	+	26627737	DLD1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.29153865659900324	[593]	[137,213,111]	-3	hSpCas9	negative selection	787705
104780160	104780183	14	+	26627737	DLD1	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.5060135501481674	[309]	[107,73,28]	-5	hSpCas9	negative selection	787706
104792615	104792638	14	-	26627737	DLD1	viability	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.22688801322255917	[541]	[159,136,137]	-2	hSpCas9	negative selection	787707
104774926	104774949	14	+	26627737	GBM cells	viability after 5 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.20542278492904997	[128]	[45,73]	-3	hSpCas9	negative selection	870017
104775153	104775176	14	-	26627737	GBM cells	viability after 5 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.1245450979383034	[247]	[117,125]	-2	hSpCas9	negative selection	870018
104775705	104775728	14	+	26627737	GBM cells	viability after 5 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.42039117851800584	[270]	[170,217]	6	hSpCas9	negative selection	870019
104776682	104776705	14	+	26627737	GBM cells	viability after 5 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.1894950993360798	[285]	[142,125]	-3	hSpCas9	negative selection	870020
104780160	104780183	14	+	26627737	GBM cells	viability after 5 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.719354712452649	[240]	[69,86]	-8	hSpCas9	negative selection	870021
104792615	104792638	14	-	26627737	GBM cells	viability after 5 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.11139113350605287	[280]	[139,138]	-2	hSpCas9	negative selection	870022
104774926	104774949	14	+	26627737	GBM cells	viability after 13 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.15622879045300442	[128]	[96,34]	-2	hSpCas9	negative selection	952332
104775153	104775176	14	-	26627737	GBM cells	viability after 13 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.33748493809854657	[247]	[142,191]	4	hSpCas9	negative selection	952333
104775705	104775728	14	+	26627737	GBM cells	viability after 13 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.8023554692726773	[270]	[358,172]	8	hSpCas9	negative selection	952334
104776682	104776705	14	+	26627737	GBM cells	viability after 13 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.3179367016672774	[285]	[129,119]	-4	hSpCas9	negative selection	952335
104780160	104780183	14	+	26627737	GBM cells	viability after 13 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.6867514496438027	[240]	[67,91]	-7	hSpCas9	negative selection	952336
104792615	104792638	14	-	26627737	GBM cells	viability after 13 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.004509870022998741	[280]	[174,134]	0	hSpCas9	negative selection	952337
104774926	104774949	14	+	26627737	GBM cells	viability after 21 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.3282263032456434	[128]	[59,51]	-3	hSpCas9	negative selection	1034647
104775153	104775176	14	-	26627737	GBM cells	viability after 21 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.38154687540354093	[247]	[174,175]	4	hSpCas9	negative selection	1034648
104775705	104775728	14	+	26627737	GBM cells	viability after 21 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.2101130076682925	[270]	[162,176]	2	hSpCas9	negative selection	1034649
104776682	104776705	14	+	26627737	GBM cells	viability after 21 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.44745126212519154	[285]	[126,101]	-4	hSpCas9	negative selection	1034650
104780160	104780183	14	+	26627737	GBM cells	viability after 21 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.1408048404699256	[240]	[100,134]	-1	hSpCas9	negative selection	1034651
104792615	104792638	14	-	26627737	GBM cells	viability after 21 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.31203018912334535	[280]	[188,189]	3	hSpCas9	negative selection	1034652
104774926	104774949	14	+	26627737	RPE1	viability after 9 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.3923430568749255	[694]	[84,269]	-3	hSpCas9	negative selection	1116962
104775153	104775176	14	-	26627737	RPE1	viability after 9 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.8016023970916171	[1243]	[284,143]	-5	hSpCas9	negative selection	1116963
104775705	104775728	14	+	26627737	RPE1	viability after 9 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.3629072019935375	[836]	[329,345]	2	hSpCas9	negative selection	1116964
104776682	104776705	14	+	26627737	RPE1	viability after 9 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.5882458234588266	[901]	[134,255]	-4	hSpCas9	negative selection	1116965
104780160	104780183	14	+	26627737	RPE1	viability after 9 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.06987679542602365	[431]	[89,208]	0	hSpCas9	negative selection	1116966
104792615	104792638	14	-	26627737	RPE1	viability after 9 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.42483783333823577	[1368]	[265,387]	-3	hSpCas9	negative selection	1116967
104774926	104774949	14	+	26627737	RPE1	viability after 12 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.5730499887899367	[694]	[108,142]	-4	hSpCas9	negative selection	1199277
104775153	104775176	14	-	26627737	RPE1	viability after 12 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.7971229140724823	[1243]	[220,161]	-5	hSpCas9	negative selection	1199278
104775705	104775728	14	+	26627737	RPE1	viability after 12 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.5068932275532639	[836]	[371,263]	3	hSpCas9	negative selection	1199279
104776682	104776705	14	+	26627737	RPE1	viability after 12 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.9302414430749403	[901]	[183,66]	-6	hSpCas9	negative selection	1199280
104780160	104780183	14	+	26627737	RPE1	viability after 12 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.4649872804949021	[431]	[86,80]	-3	hSpCas9	negative selection	1199281
104792615	104792638	14	-	26627737	RPE1	viability after 12 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.6171074710579003	[1368]	[266,210]	-4	hSpCas9	negative selection	1199282
104774926	104774949	14	+	26627737	RPE1	viability after 15 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.911386395112189	[694]	[102,105]	-5	hSpCas9	negative selection	1281592
104775153	104775176	14	-	26627737	RPE1	viability after 15 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.6291413492537985	[1243]	[197,255]	-4	hSpCas9	negative selection	1281593
104775705	104775728	14	+	26627737	RPE1	viability after 15 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.7615878886158938	[836]	[490,314]	5	hSpCas9	negative selection	1281594
104776682	104776705	14	+	26627737	RPE1	viability after 15 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.2671603400195468	[901]	[266,158]	-2	hSpCas9	negative selection	1281595
104780160	104780183	14	+	26627737	RPE1	viability after 15 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.4070244837816476	[431]	[82,100]	-3	hSpCas9	negative selection	1281596
104792615	104792638	14	-	26627737	RPE1	viability after 15 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.05938833967155732	[1368]	[485,323]	0	hSpCas9	negative selection	1281597
104774926	104774949	14	+	26627737	RPE1	viability after 18 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.03624852153453728	[694]	[150,216]	0	hSpCas9	negative selection	1363907
104775153	104775176	14	-	26627737	RPE1	viability after 18 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-1.274130382360525	[1243]	[176,97]	-7	hSpCas9	negative selection	1363908
104775705	104775728	14	+	26627737	RPE1	viability after 18 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.4081946883534724	[836]	[306,291]	2	hSpCas9	negative selection	1363909
104776682	104776705	14	+	26627737	RPE1	viability after 18 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.5288249311207011	[901]	[145,192]	-3	hSpCas9	negative selection	1363910
104780160	104780183	14	+	26627737	RPE1	viability after 18 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.6780196407685842	[431]	[68,76]	-4	hSpCas9	negative selection	1363911
104792615	104792638	14	-	26627737	RPE1	viability after 18 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.2693279067938802	[1368]	[394,211]	-2	hSpCas9	negative selection	1363912
104774926	104774949	14	+	26627737	HeLa	viability after 8 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-1.5950057218072253	[180]	[8,2,59]	-9	hSpCas9	negative selection	1446222
104775153	104775176	14	-	26627737	HeLa	viability after 8 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.33474714501203556	[478]	[144,175,169]	-3	hSpCas9	negative selection	1446223
104775705	104775728	14	+	26627737	HeLa	viability after 8 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.2650044815039895	[427]	[404,137,171]	2	hSpCas9	negative selection	1446224
104776682	104776705	14	+	26627737	HeLa	viability after 8 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.8350135793433175	[250]	[216,171,199]	7	hSpCas9	negative selection	1446225
104780160	104780183	14	+	26627737	HeLa	viability after 8 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.01484091516113406	[325]	[224,124,100]	0	hSpCas9	negative selection	1446226
104792615	104792638	14	-	26627737	HeLa	viability after 8 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.6872906832388744	[482]	[375,300,345]	6	hSpCas9	negative selection	1446227
104774926	104774949	14	+	26627737	HeLa	viability after 12 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.2153824361228811	[180]	[28,113,43]	-2	hSpCas9	negative selection	1528537
104775153	104775176	14	-	26627737	HeLa	viability after 12 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.601296260297306	[478]	[263,76,59]	-5	hSpCas9	negative selection	1528538
104775705	104775728	14	+	26627737	HeLa	viability after 12 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.3234665488558909	[427]	[159,279,215]	3	hSpCas9	negative selection	1528539
104776682	104776705	14	+	26627737	HeLa	viability after 12 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.3661950599984895	[250]	[103,135,159]	3	hSpCas9	negative selection	1528540
104780160	104780183	14	+	26627737	HeLa	viability after 12 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.14413011021554978	[325]	[47,136,176]	-1	hSpCas9	negative selection	1528541
104792615	104792638	14	-	26627737	HeLa	viability after 12 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.4363263522335239	[482]	[199,281,324]	4	hSpCas9	negative selection	1528542
104774926	104774949	14	+	26627737	HeLa	viability after 15 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.412948327844779	[180]	[38,98,25]	-4	hSpCas9	negative selection	1610852
104775153	104775176	14	-	26627737	HeLa	viability after 15 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.17590344821174192	[478]	[253,111,148]	-1	hSpCas9	negative selection	1610853
104775705	104775728	14	+	26627737	HeLa	viability after 15 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.701963712763359	[427]	[212,272,371]	5	hSpCas9	negative selection	1610854
104776682	104776705	14	+	26627737	HeLa	viability after 15 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	1.4580509934967603	[250]	[194,322,328]	8	hSpCas9	negative selection	1610855
104780160	104780183	14	+	26627737	HeLa	viability after 15 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.7113647573239188	[325]	[98,49,94]	-6	hSpCas9	negative selection	1610856
104792615	104792638	14	-	26627737	HeLa	viability after 15 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.2171289555308511	[482]	[135,308,242]	1	hSpCas9	negative selection	1610857
104774926	104774949	14	+	26627737	HeLa	viability after 18 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.4257003872108942	[180]	[25,80,25]	-4	hSpCas9	negative selection	1693167
104775153	104775176	14	-	26627737	HeLa	viability after 18 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.11292085400848803	[478]	[185,91,148]	-1	hSpCas9	negative selection	1693168
104775705	104775728	14	+	26627737	HeLa	viability after 18 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.7290350039454574	[427]	[150,228,371]	6	hSpCas9	negative selection	1693169
104776682	104776705	14	+	26627737	HeLa	viability after 18 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	1.4431783467709405	[250]	[123,268,328]	8	hSpCas9	negative selection	1693170
104780160	104780183	14	+	26627737	HeLa	viability after 18 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.7554674571297515	[325]	[61,40,94]	-6	hSpCas9	negative selection	1693171
104792615	104792638	14	-	26627737	HeLa	viability after 18 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.2114732749754562	[482]	[90,253,242]	1	hSpCas9	negative selection	1693172
104774926	104774949	14	+	26627737	HCT116	viability after 6 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-2.4927097297817284	[431]	[13,10]	-8	hSpCas9	negative selection	1775482
104775153	104775176	14	-	26627737	HCT116	viability after 6 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.5351547461937414	[1288]	[309,45]	-3	hSpCas9	negative selection	1775483
104775705	104775728	14	+	26627737	HCT116	viability after 6 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.5455139757554317	[691]	[158,404]	7	hSpCas9	negative selection	1775484
104776682	104776705	14	+	26627737	HCT116	viability after 6 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	1.2694797884734093	[591]	[331,164]	6	hSpCas9	negative selection	1775485
104780160	104780183	14	+	26627737	HCT116	viability after 6 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.772941209883348	[569]	[74,39]	-4	hSpCas9	negative selection	1775486
104792615	104792638	14	-	26627737	HCT116	viability after 6 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.47404538108065886	[848]	[224,24]	-3	hSpCas9	negative selection	1775487
104774926	104774949	14	+	26627737	HCT116	viability after 8 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.6212547744919283	[218]	[80,42,110]	-6	hSpCas9	negative selection	1857797
104775153	104775176	14	-	26627737	HCT116	viability after 8 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.8731930243750737	[478]	[150,185,95]	-8	hSpCas9	negative selection	1857798
104775705	104775728	14	+	26627737	HCT116	viability after 8 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.255495384023944	[381]	[269,384,100]	3	hSpCas9	negative selection	1857799
104776682	104776705	14	+	26627737	HCT116	viability after 8 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.2125009854248492	[394]	[181,157,222]	-2	hSpCas9	negative selection	1857800
104780160	104780183	14	+	26627737	HCT116	viability after 8 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.0678029993730433	[262]	[172,151,90]	0	hSpCas9	negative selection	1857801
104792615	104792638	14	-	26627737	HCT116	viability after 8 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.8114633100573975	[433]	[99,76,229]	-7	hSpCas9	negative selection	1857802
104774926	104774949	14	+	26627737	HCT116	viability after 9 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	0.2642667068526278	[431]	[99,168]	2	hSpCas9	negative selection	1940112
104775153	104775176	14	-	26627737	HCT116	viability after 9 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.15398622623314995	[1288]	[534,130]	-1	hSpCas9	negative selection	1940113
104775705	104775728	14	+	26627737	HCT116	viability after 9 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	-0.3411498099395674	[691]	[232,76]	-2	hSpCas9	negative selection	1940114
104776682	104776705	14	+	26627737	HCT116	viability after 9 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.1417773790633012	[591]	[173,118]	-1	hSpCas9	negative selection	1940115
104780160	104780183	14	+	26627737	HCT116	viability after 9 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.4891464093763797	[569]	[240,191]	3	hSpCas9	negative selection	1940116
104792615	104792638	14	-	26627737	HCT116	viability after 9 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.39421605422193007	[848]	[100,228]	-3	hSpCas9	negative selection	1940117
104774926	104774949	14	+	26627737	HCT116	viability after 12 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.3837782641362562	[218]	[112,110,38]	-4	hSpCas9	negative selection	2022427
104775153	104775176	14	-	26627737	HCT116	viability after 12 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-1.0451355901068136	[478]	[73,155,140]	-8	hSpCas9	negative selection	2022428
104775705	104775728	14	+	26627737	HCT116	viability after 12 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.36835031252367456	[381]	[311,308,152]	3	hSpCas9	negative selection	2022429
104776682	104776705	14	+	26627737	HCT116	viability after 12 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.30402123298807715	[394]	[98,188,222]	-3	hSpCas9	negative selection	2022430
104780160	104780183	14	+	26627737	HCT116	viability after 12 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.17480349542202056	[262]	[85,141,142]	-2	hSpCas9	negative selection	2022431
104792615	104792638	14	-	26627737	HCT116	viability after 12 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.7622507795217334	[433]	[83,140,182]	-7	hSpCas9	negative selection	2022432
104774926	104774949	14	+	26627737	HCT116	viability after 15 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.1227005828990329	[218]	[129,76,140]	-1	hSpCas9	negative selection	2104742
104775153	104775176	14	-	26627737	HCT116	viability after 15 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-1.3128615331323399	[478]	[94,101,132]	-8	hSpCas9	negative selection	2104743
104775705	104775728	14	+	26627737	HCT116	viability after 15 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.02457734081250651	[381]	[247,244,179]	0	hSpCas9	negative selection	2104744
104776682	104776705	14	+	26627737	HCT116	viability after 15 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.5414197318453775	[394]	[154,115,195]	-5	hSpCas9	negative selection	2104745
104780160	104780183	14	+	26627737	HCT116	viability after 15 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.6040780189019571	[262]	[199,64,45]	-6	hSpCas9	negative selection	2104746
104792615	104792638	14	-	26627737	HCT116	viability after 15 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.6424629207074495	[433]	[104,177,188]	-6	hSpCas9	negative selection	2104747
104774926	104774949	14	+	26627737	HCT116	viability after 18 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.09566627426032409	[218]	[172,87,33]	-1	hSpCas9	negative selection	2187057
104775153	104775176	14	-	26627737	HCT116	viability after 18 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-1.0680050524323241	[478]	[103,81,148]	-7	hSpCas9	negative selection	2187058
104775705	104775728	14	+	26627737	HCT116	viability after 18 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.48597928558417447	[381]	[362,300,101]	4	hSpCas9	negative selection	2187059
104776682	104776705	14	+	26627737	HCT116	viability after 18 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.5642793077839161	[394]	[128,132,124]	-5	hSpCas9	negative selection	2187060
104780160	104780183	14	+	26627737	HCT116	viability after 18 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.5239042870043527	[262]	[100,119,39]	-5	hSpCas9	negative selection	2187061
104792615	104792638	14	-	26627737	HCT116	viability after 18 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.25601338982271593	[433]	[171,160,195]	-2	hSpCas9	negative selection	2187062
104780160	104780183	14	+	24336569	HL60	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.21147179091875956	[190]	[467]	2	hSpCas9	negative selection	2247329
104780165	104780188	14	-	24336569	HL60	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.08975547426028757	[348]	[693]	0	hSpCas9	negative selection	2247330
104780171	104780194	14	+	24336569	HL60	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	0.08558528686377659	[557]	[1252]	0	hSpCas9	negative selection	2247331
104780179	104780202	14	+	24336569	HL60	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	0.8060611701126911	[599]	[2219]	6	hSpCas9	negative selection	2247332
104780194	104780217	14	-	24336569	HL60	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.9054402417594206	[165]	[657]	7	hSpCas9	negative selection	2247333
104792597	104792620	14	-	24336569	HL60	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	-0.20336415405517472	[468]	[861]	-2	hSpCas9	negative selection	2247334
104792611	104792634	14	-	24336569	HL60	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	0.465791706619461	[490]	[1434]	4	hSpCas9	negative selection	2247335
104792616	104792639	14	-	24336569	HL60	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	0.012101203605496935	[790]	[1687]	0	hSpCas9	negative selection	2247336
104792623	104792646	14	+	24336569	HL60	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	0.8592247034296876	[359]	[1381]	7	hSpCas9	negative selection	2247337
104792631	104792654	14	-	24336569	HL60	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	0.2496902139245798	[716]	[1803]	2	hSpCas9	negative selection	2247338
104780160	104780183	14	+	24336569	KBM7	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.6818838085493818	[228]	[1001]	6	hSpCas9	negative selection	2314506
104780165	104780188	14	-	24336569	KBM7	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.11979158372205612	[687]	[1726]	-1	hSpCas9	negative selection	2314507
104780171	104780194	14	+	24336569	KBM7	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	0.8308808697623706	[437]	[2124]	7	hSpCas9	negative selection	2314508
104780179	104780202	14	+	24336569	KBM7	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	1.5925224619194451	[509]	[4194]	9	hSpCas9	negative selection	2314509
104780194	104780217	14	-	24336569	KBM7	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.02226068796854816	[451]	[1251]	0	hSpCas9	negative selection	2314510
104792597	104792620	14	-	24336569	KBM7	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	0.25949176613144215	[550]	[1798]	2	hSpCas9	negative selection	2314511
104792611	104792634	14	-	24336569	KBM7	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	0.295645388089006	[344]	[1154]	2	hSpCas9	negative selection	2314512
104792616	104792639	14	-	24336569	KBM7	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	0.656264786284913	[612]	[2634]	6	hSpCas9	negative selection	2314513
104792623	104792646	14	+	24336569	KBM7	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	0.9838859640468933	[321]	[1736]	8	hSpCas9	negative selection	2314514
104792631	104792654	14	-	24336569	KBM7	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	-0.18310855859956976	[752]	[1808]	-1	hSpCas9	negative selection	2314515
104776259	104776282	14	+	25494202	A375	resistance to PLX-4720 (puromycin)	GCCTCAGCCTCCCAAGTAGCCGG	AKT1	ENSG00000142208	0.6911400425855385	[25,30]	[5,13]	3	dCas9-VP64	positive selection	2396532
104793769	104793792	14	-	25494202	A375	resistance to PLX-4720 (puromycin)	ATGCCTGTAGTTGGTGATGTGGG	AKT1	ENSG00000142208	-0.8830731058491897	[517,39]	[51,10]	-5	dCas9-VP64	positive selection	2437333
104793744	104793767	14	-	25494202	A375	resistance to PLX-4720 (puromycin)	CTGGGTTTTGAGGGATGCCTAGG	AKT1	ENSG00000142208	4.046051017477945	[24,41]	[234,2]	9	dCas9-VP64	positive selection	2437334
104793691	104793714	14	+	25494202	A375	resistance to PLX-4720 (puromycin)	CTTTATCCATTGATCCTGCATGG	AKT1	ENSG00000142208	-0.27986646236725	[309,98]	[59,10]	-1	dCas9-VP64	positive selection	2437335
104776259	104776282	14	+	25494202	A375	resistance to PLX-4720 (zeocin)	GCCTCAGCCTCCCAAGTAGCCGG	AKT1	ENSG00000142208	0.4030393266100577	[90,274]	[19,131]	3	dCas9-VP64	positive selection	2491787
104793769	104793792	14	-	25494202	A375	resistance to PLX-4720 (zeocin)	ATGCCTGTAGTTGGTGATGTGGG	AKT1	ENSG00000142208	-0.6705762757081435	[87,65]	[14,15]	-6	dCas9-VP64	positive selection	2532588
104793744	104793767	14	-	25494202	A375	resistance to PLX-4720 (zeocin)	CTGGGTTTTGAGGGATGCCTAGG	AKT1	ENSG00000142208	-0.4577251959797122	[15,128]	[14,17]	-5	dCas9-VP64	positive selection	2532589
104793691	104793714	14	+	25494202	A375	resistance to PLX-4720 (zeocin)	CTTTATCCATTGATCCTGCATGG	AKT1	ENSG00000142208	0.43308863015792487	[137,142]	[53,68]	3	dCas9-VP64	positive selection	2532590
104792616	104792639	14	-	27260157	DLD1	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	-0.0669851928360129	[]	[]	0	hSpCas9	negative selection	2574511
104775705	104775728	14	+	27260157	DLD1	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.109359371532196	[]	[]	3	hSpCas9	negative selection	2575135
104780171	104780194	14	+	27260157	DLD1	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	-0.849260296841084	[]	[]	-6	hSpCas9	negative selection	2576845
104773541	104773564	14	+	27260157	DLD1	viability	CCTCGGAGAACACACGCTCCCGG	AKT1	ENSG00000142208	1.09821205147484	[]	[]	9	hSpCas9	negative selection	2580926
104780194	104780217	14	-	27260157	DLD1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.000604021968651692	[]	[]	0	hSpCas9	negative selection	2582028
104780115	104780138	14	-	27260157	DLD1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.107451996517769	[]	[]	-1	hSpCas9	negative selection	2585537
104776682	104776705	14	+	27260157	DLD1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-2.62958912889174	[]	[]	-9	hSpCas9	negative selection	2586276
104792623	104792646	14	+	27260157	DLD1	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	0.164403266432073	[]	[]	3	hSpCas9	negative selection	2586819
104792611	104792634	14	-	27260157	DLD1	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	-0.442516932314748	[]	[]	-4	hSpCas9	negative selection	2607331
104780160	104780183	14	+	27260157	DLD1	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.576941292335485	[]	[]	-5	hSpCas9	negative selection	2607755
104780165	104780188	14	-	27260157	DLD1	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.256575858580944	[]	[]	-2	hSpCas9	negative selection	2610336
104774970	104774993	14	+	27260157	DLD1	viability	CGCGGTTCTCGGTGAGTGTGTGG	AKT1	ENSG00000142208	-0.778801613556879	[]	[]	-6	hSpCas9	negative selection	2618646
104780179	104780202	14	+	27260157	DLD1	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	0.16592663956482	[]	[]	3	hSpCas9	negative selection	2619566
104775153	104775176	14	-	27260157	DLD1	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.703274789418852	[]	[]	-5	hSpCas9	negative selection	2620290
104780088	104780111	14	+	27260157	DLD1	viability	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.526778228661656	[]	[]	-4	hSpCas9	negative selection	2622514
104792597	104792620	14	-	27260157	DLD1	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	-0.696487759472013	[]	[]	-5	hSpCas9	negative selection	2641076
104792631	104792654	14	-	27260157	DLD1	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	0.290133094560418	[]	[]	4	hSpCas9	negative selection	2646111
104773926	104773949	14	+	27260157	DLD1	viability	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.382823940664818	[]	[]	-3	hSpCas9	negative selection	2647649
104775719	104775742	14	-	27260157	DLD1	viability	GAGGAGATGGACTTCCGGTCGGG	AKT1	ENSG00000142208	0.457807951839335	[]	[]	7	hSpCas9	negative selection	2647892
104792616	104792639	14	-	27260157	HT1080	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	-0.277924776054888	[]	[]	-4	hSpCas9	negative selection	2650641
104775705	104775728	14	+	27260157	HT1080	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.727417914341701	[]	[]	6	hSpCas9	negative selection	2651265
104780171	104780194	14	+	27260157	HT1080	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	0.0353726285481358	[]	[]	0	hSpCas9	negative selection	2652975
104773541	104773564	14	+	27260157	HT1080	viability	CCTCGGAGAACACACGCTCCCGG	AKT1	ENSG00000142208	1.18662952542449	[]	[]	8	hSpCas9	negative selection	2657056
104780194	104780217	14	-	27260157	HT1080	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.219428260079449	[]	[]	0	hSpCas9	negative selection	2658158
104780115	104780138	14	-	27260157	HT1080	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.448294541778248	[]	[]	4	hSpCas9	negative selection	2661667
104776682	104776705	14	+	27260157	HT1080	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.436899149778987	[]	[]	-7	hSpCas9	negative selection	2662406
104792623	104792646	14	+	27260157	HT1080	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	0.620381931431699	[]	[]	5	hSpCas9	negative selection	2662949
104792611	104792634	14	-	27260157	HT1080	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	-0.507967647772925	[]	[]	-7	hSpCas9	negative selection	2683461
104780160	104780183	14	+	27260157	HT1080	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.466298861581501	[]	[]	-7	hSpCas9	negative selection	2683885
104780165	104780188	14	-	27260157	HT1080	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.214678268773973	[]	[]	0	hSpCas9	negative selection	2686466
104774970	104774993	14	+	27260157	HT1080	viability	CGCGGTTCTCGGTGAGTGTGTGG	AKT1	ENSG00000142208	-0.295315464663498	[]	[]	-7	hSpCas9	negative selection	2694776
104780179	104780202	14	+	27260157	HT1080	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	0.559557197249954	[]	[]	5	hSpCas9	negative selection	2695696
104775153	104775176	14	-	27260157	HT1080	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-1.25315873714434	[]	[]	-9	hSpCas9	negative selection	2696420
104780088	104780111	14	+	27260157	HT1080	viability	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	0.253761369054915	[]	[]	2	hSpCas9	negative selection	2698644
104792597	104792620	14	-	27260157	HT1080	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	-1.15425881746738	[]	[]	-8	hSpCas9	negative selection	2717206
104792631	104792654	14	-	27260157	HT1080	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	0.221890144363706	[]	[]	2	hSpCas9	negative selection	2722241
104773926	104773949	14	+	27260157	HT1080	viability	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.00585324365936244	[]	[]	0	hSpCas9	negative selection	2723779
104775719	104775742	14	-	27260157	HT1080	viability	GAGGAGATGGACTTCCGGTCGGG	AKT1	ENSG00000142208	0.939267669467807	[]	[]	7	hSpCas9	negative selection	2724022
104792616	104792639	14	-	27260157	MKN45	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	-0.0226473965125511	[]	[]	0	hSpCas9	negative selection	2726771
104775705	104775728	14	+	27260157	MKN45	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.436756106779048	[]	[]	6	hSpCas9	negative selection	2727395
104780171	104780194	14	+	27260157	MKN45	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	0.657203162296419	[]	[]	7	hSpCas9	negative selection	2729105
104773541	104773564	14	+	27260157	MKN45	viability	CCTCGGAGAACACACGCTCCCGG	AKT1	ENSG00000142208	1.09986252960997	[]	[]	8	hSpCas9	negative selection	2733186
104780194	104780217	14	-	27260157	MKN45	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.466026242938465	[]	[]	6	hSpCas9	negative selection	2734288
104780115	104780138	14	-	27260157	MKN45	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.239702224750311	[]	[]	-1	hSpCas9	negative selection	2737797
104776682	104776705	14	+	27260157	MKN45	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.885542083239218	[]	[]	-7	hSpCas9	negative selection	2738536
104792623	104792646	14	+	27260157	MKN45	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	0.710314222451473	[]	[]	7	hSpCas9	negative selection	2739079
104792611	104792634	14	-	27260157	MKN45	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	-0.21616571437736	[]	[]	-1	hSpCas9	negative selection	2759591
104780160	104780183	14	+	27260157	MKN45	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.11648161589258	[]	[]	0	hSpCas9	negative selection	2760015
104780165	104780188	14	-	27260157	MKN45	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.124679830784063	[]	[]	0	hSpCas9	negative selection	2762596
104774970	104774993	14	+	27260157	MKN45	viability	CGCGGTTCTCGGTGAGTGTGTGG	AKT1	ENSG00000142208	-0.299702696687364	[]	[]	-1	hSpCas9	negative selection	2770906
104780179	104780202	14	+	27260157	MKN45	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	0.702010361512882	[]	[]	7	hSpCas9	negative selection	2771826
104775153	104775176	14	-	27260157	MKN45	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.908891807572277	[]	[]	8	hSpCas9	negative selection	2772550
104780088	104780111	14	+	27260157	MKN45	viability	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	0.487789282613569	[]	[]	6	hSpCas9	negative selection	2774774
104792597	104792620	14	-	27260157	MKN45	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	0.59416572817923	[]	[]	7	hSpCas9	negative selection	2793336
104792631	104792654	14	-	27260157	MKN45	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	0.160298972442749	[]	[]	5	hSpCas9	negative selection	2798371
104773926	104773949	14	+	27260157	MKN45	viability	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.566747531365782	[]	[]	-6	hSpCas9	negative selection	2799909
104775719	104775742	14	-	27260157	MKN45	viability	GAGGAGATGGACTTCCGGTCGGG	AKT1	ENSG00000142208	-0.227794370477307	[]	[]	-1	hSpCas9	negative selection	2800152
104792616	104792639	14	-	27260157	RKO	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	-0.0739644829903538	[]	[]	0	hSpCas9	negative selection	2802901
104775705	104775728	14	+	27260157	RKO	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.292234794115204	[]	[]	1	hSpCas9	negative selection	2803525
104780171	104780194	14	+	27260157	RKO	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	-0.0440785332349306	[]	[]	0	hSpCas9	negative selection	2805235
104773541	104773564	14	+	27260157	RKO	viability	CCTCGGAGAACACACGCTCCCGG	AKT1	ENSG00000142208	0.223067069504902	[]	[]	1	hSpCas9	negative selection	2809316
104780194	104780217	14	-	27260157	RKO	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.140558744327489	[]	[]	0	hSpCas9	negative selection	2810418
104780115	104780138	14	-	27260157	RKO	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.281760387817224	[]	[]	1	hSpCas9	negative selection	2813927
104776682	104776705	14	+	27260157	RKO	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.978693151690017	[]	[]	-8	hSpCas9	negative selection	2814666
104792623	104792646	14	+	27260157	RKO	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	-0.149468878139192	[]	[]	0	hSpCas9	negative selection	2815209
104792611	104792634	14	-	27260157	RKO	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	-0.903279666136752	[]	[]	-8	hSpCas9	negative selection	2835721
104780160	104780183	14	+	27260157	RKO	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-1.09791964766859	[]	[]	-8	hSpCas9	negative selection	2836145
104780165	104780188	14	-	27260157	RKO	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	0.350381926139992	[]	[]	1	hSpCas9	negative selection	2838726
104774970	104774993	14	+	27260157	RKO	viability	CGCGGTTCTCGGTGAGTGTGTGG	AKT1	ENSG00000142208	0.251714322211486	[]	[]	1	hSpCas9	negative selection	2847036
104780179	104780202	14	+	27260157	RKO	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	1.18094863123081	[]	[]	9	hSpCas9	negative selection	2847956
104775153	104775176	14	-	27260157	RKO	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.877805358449396	[]	[]	-8	hSpCas9	negative selection	2848680
104780088	104780111	14	+	27260157	RKO	viability	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	0.0485884899933382	[]	[]	0	hSpCas9	negative selection	2850904
104792597	104792620	14	-	27260157	RKO	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	-1.13487238882079	[]	[]	-8	hSpCas9	negative selection	2869466
104792631	104792654	14	-	27260157	RKO	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	-0.147440617716223	[]	[]	0	hSpCas9	negative selection	2874501
104773926	104773949	14	+	27260157	RKO	viability	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	0.0482559364174477	[]	[]	0	hSpCas9	negative selection	2876039
104775719	104775742	14	-	27260157	RKO	viability	GAGGAGATGGACTTCCGGTCGGG	AKT1	ENSG00000142208	0.396149860533061	[]	[]	2	hSpCas9	negative selection	2876282
104792616	104792639	14	-	27260157	SF268	viability	CGACGTGGCTATTGTGAAGGAGG	AKT1	ENSG00000142208	-0.566769703460039	[]	[]	-4	hSpCas9	negative selection	2879031
104775705	104775728	14	+	27260157	SF268	viability	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	0.285824365699698	[]	[]	4	hSpCas9	negative selection	2879655
104780171	104780194	14	+	27260157	SF268	viability	TTCTTGAGGAGGAAGTAGCGTGG	AKT1	ENSG00000142208	-0.0577065425911224	[]	[]	0	hSpCas9	negative selection	2881365
104773541	104773564	14	+	27260157	SF268	viability	CCTCGGAGAACACACGCTCCCGG	AKT1	ENSG00000142208	-0.164057833784404	[]	[]	-1	hSpCas9	negative selection	2885446
104780194	104780217	14	-	27260157	SF268	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.50956368327757	[]	[]	7	hSpCas9	negative selection	2886548
104780115	104780138	14	-	27260157	SF268	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.284635033517792	[]	[]	-2	hSpCas9	negative selection	2890057
104776682	104776705	14	+	27260157	SF268	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-3.14987578538108	[]	[]	-9	hSpCas9	negative selection	2890796
104792623	104792646	14	+	27260157	SF268	viability	CACAATAGCCACGTCGCTCATGG	AKT1	ENSG00000142208	-0.107362205639324	[]	[]	-1	hSpCas9	negative selection	2891339
104792611	104792634	14	-	27260157	SF268	viability	TGGCTATTGTGAAGGAGGGTTGG	AKT1	ENSG00000142208	-0.21174126487311	[]	[]	-2	hSpCas9	negative selection	2911851
104780160	104780183	14	+	27260157	SF268	viability	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.312855995099294	[]	[]	-2	hSpCas9	negative selection	2912275
104780165	104780188	14	-	27260157	SF268	viability	TACTTCCTCCTCAAGAATGATGG	AKT1	ENSG00000142208	-0.498721797220234	[]	[]	-4	hSpCas9	negative selection	2914856
104774970	104774993	14	+	27260157	SF268	viability	CGCGGTTCTCGGTGAGTGTGTGG	AKT1	ENSG00000142208	-0.675711488001186	[]	[]	-5	hSpCas9	negative selection	2923166
104780179	104780202	14	+	27260157	SF268	viability	GAGGAAGTAGCGTGGCCGCCAGG	AKT1	ENSG00000142208	0.920231471544345	[]	[]	9	hSpCas9	negative selection	2924086
104775153	104775176	14	-	27260157	SF268	viability	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.15585025330094	[]	[]	-1	hSpCas9	negative selection	2924810
104780088	104780111	14	+	27260157	SF268	viability	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.706945425756651	[]	[]	-5	hSpCas9	negative selection	2927034
104792597	104792620	14	-	27260157	SF268	viability	GAGGGTTGGCTGCACAAACGAGG	AKT1	ENSG00000142208	-1.50522327535961	[]	[]	-8	hSpCas9	negative selection	2945596
104792631	104792654	14	-	27260157	SF268	viability	CTCGGGCACCATGAGCGACGTGG	AKT1	ENSG00000142208	-0.63430923679086	[]	[]	-5	hSpCas9	negative selection	2950631
104773926	104773949	14	+	27260157	SF268	viability	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.310171127366502	[]	[]	-2	hSpCas9	negative selection	2952169
104775719	104775742	14	-	27260157	SF268	viability	GAGGAGATGGACTTCCGGTCGGG	AKT1	ENSG00000142208	0.144773808732697	[]	[]	0	hSpCas9	negative selection	2952412
104775099	104775122	14	+	27013184	SW480	treatment with recombinant TRAIL	TGAGGATCTTCATGGCGTAGTAG	AKT1	ENSG00000142208	-1.0751277941419064	[73,47]	[1,27]	-5	hSpCas9	positive selection	2963449
104773927	104773950	14	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	0.45389604970406205	[3,4]	[3,5]	8	hSpCas9	positive selection	2969768
104792615	104792638	14	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.03305948360245656	[3,3]	[2,2]	0	hSpCas9	positive selection	2969769
104776661	104776684	14	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	-0.7270133559993538	[3,3]	[3,0]	-8	hSpCas9	positive selection	2969770
104775651	104775674	14	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	-0.722439297080062	[2,2]	[1,0]	-8	hSpCas9	positive selection	2969771
104773927	104773950	14	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.30071723932152555	[3,3]	[3,0]	-4	hSpCas9	positive selection	3037626
104792615	104792638	14	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.5821800685701052	[3,3]	[3,3]	8	hSpCas9	positive selection	3037627
104776661	104776684	14	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	0.21662278499675025	[3,3]	[3,2]	4	hSpCas9	positive selection	3037628
104775651	104775674	14	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	0.31880222713678713	[2,2]	[3,0]	5	hSpCas9	positive selection	3037629
104774953	104774976		-	24336571	A375	resistance to PLX after 7 days	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.05916870899312904	[14,14]	[13,16]	2	hSpCas9	positive selection	3112496
104774927	104774950		+	24336571	A375	resistance to PLX after 7 days	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	-0.2534106238077035	[25,25]	[22,19]	-7	hSpCas9	positive selection	3112507
104780115	104780138		-	24336571	A375	resistance to PLX after 7 days	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.20473601186723175	[12,13]	[10,11]	-6	hSpCas9	positive selection	3112519
104780088	104780111		+	24336571	A375	resistance to PLX after 7 days	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.4230476021206835	[7,5]	[4,4]	-9	hSpCas9	positive selection	3112530
104774953	104774976		-	24336571	A375	resistance to PLX after 14 days	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.382772715213888	[14,12]	[7,7]	4	hSpCas9	positive selection	3170289
104774927	104774950		+	24336571	A375	resistance to PLX after 14 days	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	-0.6792818437364355	[26,17]	[5,6]	-7	hSpCas9	positive selection	3170300
104780115	104780138		-	24336571	A375	resistance to PLX after 14 days	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.06706707466875089	[8,12]	[3,5]	0	hSpCas9	positive selection	3170312
104780088	104780111		+	24336571	A375	resistance to PLX after 14 days	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.05132979646051994	[7,3]	[1,2]	0	hSpCas9	positive selection	3170323
104774953	104774976		-	24336571	A375	viability after 7 days	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.09356401659471364	[16]	[14,14]	-1	hSpCas9	negative selection	3228082
104774927	104774950		+	24336571	A375	viability after 7 days	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	0.33041727961947576	[21]	[25,25]	5	hSpCas9	negative selection	3228093
104780115	104780138		-	24336571	A375	viability after 7 days	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.32784396932251364	[17]	[12,13]	-5	hSpCas9	negative selection	3228105
104780088	104780111		+	24336571	A375	viability after 7 days	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.09892178089651726	[7]	[7,5]	-1	hSpCas9	negative selection	3228116
104774953	104774976		-	24336571	A375	viability after 14 days	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.1815752523975882	[16]	[14,12]	-2	hSpCas9	negative selection	3285875
104774927	104774950		+	24336571	A375	viability after 14 days	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	0.14944085419284925	[21]	[26,17]	2	hSpCas9	negative selection	3285886
104780115	104780138		-	24336571	A375	viability after 14 days	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.557897999199506	[17]	[8,12]	-6	hSpCas9	negative selection	3285898
104780088	104780111		+	24336571	A375	viability after 14 days	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.31047636495776065	[7]	[7,3]	-4	hSpCas9	negative selection	3285909
104773272	104773295	14	+	27383988	293T	resistance to West Nile virus (flavivirus)	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.1845674537002087	[152,115]	[0,0]	1	hSpCas9	positive selection	3336184
104780117	104780140	14	+	27383988	293T	resistance to West Nile virus (flavivirus)	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.5295946759126771	[197,243]	[0,0]	-3	hSpCas9	positive selection	3336185
104780115	104780138	14	-	27383988	293T	resistance to West Nile virus (flavivirus)	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.21293209208738176	[131,131]	[0,0]	1	hSpCas9	positive selection	3336186
104773349	104773372	14	+	27383988	293T	resistance to West Nile virus (flavivirus)	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.7495315712471388	[90,90]	[0,0]	3	hSpCas9	positive selection	3355437
104774952	104774975	14	+	27383988	293T	resistance to West Nile virus (flavivirus)	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.6426163673306269	[97,97]	[0,0]	3	hSpCas9	positive selection	3355438
104774953	104774976	14	-	27383988	293T	resistance to West Nile virus (flavivirus)	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.07741712143090065	[144,144]	[0,0]	0	hSpCas9	positive selection	3355439
104773926	104773949	14	+	26780180	HT29	viability (Avana library 4 designs)	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	0.538329384499566	[]	[]	2	hSpCas9	negative selection	3405846
104792615	104792638	14	-	26780180	HT29	viability (Avana library 4 designs)	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.953840682482765	[]	[]	3	hSpCas9	negative selection	3405847
104776660	104776683	14	+	26780180	HT29	viability (Avana library 4 designs)	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	0.737315943748268	[]	[]	3	hSpCas9	negative selection	3405848
104775651	104775674	14	-	26780180	HT29	viability (Avana library 4 designs)	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	0.214208140346947	[]	[]	0	hSpCas9	negative selection	3405849
104776660	104776683	14	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	3.81711464131766	[]	[]	6	hSpCas9	negative selection	3478064
104775651	104775674	14	-	26780180	A375	viability (Avana lentiCRISPRv2)	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	0.583492519593947	[]	[]	0	hSpCas9	negative selection	3478065
104792615	104792638	14	-	26780180	A375	viability (Avana lentiCRISPRv2)	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	6.66223630410329	[]	[]	9	hSpCas9	negative selection	3478066
104773926	104773949	14	+	26780180	A375	viability (Avana lentiCRISPRv2)	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-5.69108755476244	[]	[]	-6	hSpCas9	negative selection	3478067
104775692	104775715	14	+	26780180	A375	viability (Avana lentiCRISPRv2)	TCAGCCCCTGAGTTGTCACTGGG	AKT1	ENSG00000142208	-0.0304379926588212	[]	[]	0	hSpCas9	negative selection	3478068
104776658	104776681	14	-	26780180	A375	viability (Avana lentiCRISPRv2)	ATGTGGAGACTCCTGAGGAGCGG	AKT1	ENSG00000142208	4.68815677860148	[]	[]	7	hSpCas9	negative selection	3478069
104776660	104776683	14	+	26780180	A375	viability (Avana lentiGuide)	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	1.69105215450775	[]	[]	2	hSpCas9	negative selection	3586723
104775651	104775674	14	-	26780180	A375	viability (Avana lentiGuide)	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	-5.72035390862933	[]	[]	-8	hSpCas9	negative selection	3586724
104792615	104792638	14	-	26780180	A375	viability (Avana lentiGuide)	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-1.23616635030292	[]	[]	-2	hSpCas9	negative selection	3586725
104773926	104773949	14	+	26780180	A375	viability (Avana lentiGuide)	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	9.05594046927435	[]	[]	6	hSpCas9	negative selection	3586726
104775692	104775715	14	+	26780180	A375	viability (Avana lentiGuide)	TCAGCCCCTGAGTTGTCACTGGG	AKT1	ENSG00000142208	-4.32741180793093	[]	[]	-7	hSpCas9	negative selection	3586727
104776658	104776681	14	-	26780180	A375	viability (Avana lentiGuide)	ATGTGGAGACTCCTGAGGAGCGG	AKT1	ENSG00000142208	-0.30397515174587	[]	[]	0	hSpCas9	negative selection	3586728
104776660	104776683	14	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	0.3059672313431828	[4]	[5,5]	8	hSpCas9	positive selection	3695382
104775651	104775674	14	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	0.36748325307106605	[3]	[5,4]	9	hSpCas9	positive selection	3695383
104792615	104792638	14	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.22760522976866676	[4]	[5,5]	6	hSpCas9	positive selection	3695384
104773926	104773949	14	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.10562255246737184	[4]	[5,3]	-2	hSpCas9	positive selection	3695385
104775692	104775715	14	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TCAGCCCCTGAGTTGTCACTGGG	AKT1	ENSG00000142208	0.30287172515443855	[4]	[6,5]	8	hSpCas9	positive selection	3695386
104776658	104776681	14	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ATGTGGAGACTCCTGAGGAGCGG	AKT1	ENSG00000142208	0.0521892474810025	[5]	[4,5]	1	hSpCas9	positive selection	3695387
104776660	104776683	14	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCTCCTCAGGAGTCTCCACATGG	AKT1	ENSG00000142208	-0.17385385540879505	[5]	[2,4]	-2	hSpCas9	positive selection	3803989
104775651	104775674	14	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GGCCAAGCCCAAGCACCGCGTGG	AKT1	ENSG00000142208	-0.2114114717987654	[4]	[4,1]	-3	hSpCas9	positive selection	3803990
104792615	104792638	14	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.20000714203141062	[5]	[5,5]	5	hSpCas9	positive selection	3803991
104773926	104773949	14	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CGTACTCCATGACAAAGCAGAGG	AKT1	ENSG00000142208	-0.3622536188600958	[5]	[1,5]	-5	hSpCas9	positive selection	3803992
104775692	104775715	14	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TCAGCCCCTGAGTTGTCACTGGG	AKT1	ENSG00000142208	0.27567895813962195	[6]	[6,6]	7	hSpCas9	positive selection	3803993
104776658	104776681	14	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ATGTGGAGACTCCTGAGGAGCGG	AKT1	ENSG00000142208	0.08770267050255846	[5]	[5,4]	2	hSpCas9	positive selection	3803994
104774953	104774976	14	-	26780180	A375	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	1.09858273236522	[]	[]	5	hSpCas9	negative selection	3912397
104780115	104780138	14	-	26780180	A375	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.629302936969818	[]	[]	2	hSpCas9	negative selection	3912398
104773349	104773372	14	+	26780180	A375	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.814250121693494	[]	[]	3	hSpCas9	negative selection	3912399
104774952	104774975	14	+	26780180	A375	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.845241549263568	[]	[]	3	hSpCas9	negative selection	3912400
104780117	104780140	14	+	26780180	A375	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	0.923746279227631	[]	[]	4	hSpCas9	negative selection	3912401
104773272	104773295	14	+	26780180	A375	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.497581808654282	[]	[]	1	hSpCas9	negative selection	3912402
104774953	104774976	14	-	26780180	HT29	viability (GeCKOv2 library)	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	1.34344077517542	[]	[]	6	hSpCas9	negative selection	4020363
104780115	104780138	14	-	26780180	HT29	viability (GeCKOv2 library)	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	1.03350530527213	[]	[]	5	hSpCas9	negative selection	4020364
104773349	104773372	14	+	26780180	HT29	viability (GeCKOv2 library)	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	1.12508957955876	[]	[]	5	hSpCas9	negative selection	4020365
104774952	104774975	14	+	26780180	HT29	viability (GeCKOv2 library)	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	1.48876509782734	[]	[]	7	hSpCas9	negative selection	4020366
104780117	104780140	14	+	26780180	HT29	viability (GeCKOv2 library)	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	0.466403580014994	[]	[]	1	hSpCas9	negative selection	4020367
104773272	104773295	14	+	26780180	HT29	viability (GeCKOv2 library)	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.279692885574246	[]	[]	0	hSpCas9	negative selection	4020368
104774953	104774976	14	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.13140196731324238	[4]	[2,2]	-3	hSpCas9	positive selection	4135228
104774927	104774950	14	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	0.21850209111499885	[4]	[5,2]	5	hSpCas9	positive selection	4135239
104780115	104780138	14	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.02512840296977932	[3]	[2,2]	0	hSpCas9	positive selection	4135251
104780088	104780111	14	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	0.3728925210989746	[2]	[4,0]	8	hSpCas9	positive selection	4135262
104774953	104774976	14	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	1.265361143755959	[2]	[3,1,2,3]	9	hSpCas9	positive selection	4199250
104774927	104774950	14	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CTCCCACTCACTGTGAGGAAGGG	AKT1	ENSG00000142208	1.0801032986492785	[4]	[4,0,4,4]	7	hSpCas9	positive selection	4199261
104780115	104780138	14	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	1.1155232313997292	[3]	[0,1,0,5]	8	hSpCas9	positive selection	4199273
104780088	104780111	14	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GCGCCACAGAGAAGTTGTTGAGG	AKT1	ENSG00000142208	-0.019128079758347183	[1]	[0,0,1,0]	0	hSpCas9	positive selection	4199284
104773272	104773295	14	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5981522818671089	[3]	[3,3]	9	hSpCas9	positive selection	4255208
104780117	104780140	14	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	0.10065587307312962	[4]	[3,3]	2	hSpCas9	positive selection	4255209
104780115	104780138	14	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.026544669934393254	[3]	[3,1]	0	hSpCas9	positive selection	4255210
104773349	104773372	14	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.08465048680738377	[2]	[2,0]	-2	hSpCas9	positive selection	4319490
104774952	104774975	14	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.3152042015009522	[2]	[2,0]	-6	hSpCas9	positive selection	4319491
104774953	104774976	14	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.02198746893569064	[3]	[3,1]	0	hSpCas9	positive selection	4319492
104776665	104776687	14	+	27760321	OCIAML3	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	-0.6103340091083271	[290,212]	[136,115]	-5	hSpCas9	negative selection	4377234
104776683	104776705	14	+	27760321	OCIAML3	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.21778187179036562	[422,368]	[210,336]	-2	hSpCas9	negative selection	4377235
104773535	104773557	14	-	27760321	OCIAML3	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.3943682553778684	[586,628]	[330,406]	-4	hSpCas9	negative selection	4377236
104774997	104775019	14	-	27760321	OCIAML3	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	-0.17262749968118418	[715,554]	[383,507]	-2	hSpCas9	negative selection	4377237
104775698	104775720	14	-	27760321	OCIAML3	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	-2.090202030585681	[387,347]	[60,75]	-9	hSpCas9	negative selection	4377238
104776665	104776687	14	+	27760321	OCIAML2	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.1101938058059293	[290,212]	[201,238]	1	hSpCas9	negative selection	4460697
104776683	104776705	14	+	27760321	OCIAML2	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.47183718448181916	[422,368]	[226,234]	-5	hSpCas9	negative selection	4460698
104773535	104773557	14	-	27760321	OCIAML2	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.2417546182061066	[586,628]	[364,488]	-3	hSpCas9	negative selection	4460699
104774997	104775019	14	-	27760321	OCIAML2	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.47100548957045063	[715,554]	[612,831]	6	hSpCas9	negative selection	4460700
104775698	104775720	14	-	27760321	OCIAML2	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.2705809366659466	[387,347]	[299,435]	3	hSpCas9	negative selection	4460701
104776665	104776687	14	+	27760321	MV411	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	-0.7857541559201602	[290,212]	[35,184]	-5	hSpCas9	negative selection	4544160
104776683	104776705	14	+	27760321	MV411	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.580303523798098	[422,368]	[141,222]	-4	hSpCas9	negative selection	4544161
104773535	104773557	14	-	27760321	MV411	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.39155377238741274	[586,628]	[216,443]	-3	hSpCas9	negative selection	4544162
104774997	104775019	14	-	27760321	MV411	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	-0.5976207438078089	[715,554]	[231,339]	-4	hSpCas9	negative selection	4544163
104775698	104775720	14	-	27760321	MV411	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	-0.05811081544072194	[387,347]	[210,265]	0	hSpCas9	negative selection	4544164
104776665	104776687	14	+	27760321	HL60	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	-0.6875118146478323	[290,212]	[146,104]	-6	hSpCas9	negative selection	4627623
104776683	104776705	14	+	27760321	HL60	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.49492479139886764	[422,368]	[245,210]	-5	hSpCas9	negative selection	4627624
104773535	104773557	14	-	27760321	HL60	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	0.3638202224083202	[586,628]	[411,887]	4	hSpCas9	negative selection	4627625
104774997	104775019	14	-	27760321	HL60	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.031011059344294933	[715,554]	[530,521]	0	hSpCas9	negative selection	4627626
104775698	104775720	14	-	27760321	HL60	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	-0.7676075090574445	[387,347]	[171,180]	-7	hSpCas9	negative selection	4627627
104776665	104776687	14	+	27760321	HT1080	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	2.147701141735357	[212,165]	[407,253]	9	hSpCas9	negative selection	4711086
104776683	104776705	14	+	27760321	HT1080	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.0937886738458559	[368,287]	[163,112]	0	hSpCas9	negative selection	4711087
104773535	104773557	14	-	27760321	HT1080	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-1.6270956739235825	[628,490]	[97,43]	-5	hSpCas9	negative selection	4711088
104774997	104775019	14	-	27760321	HT1080	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	-1.4396036494300821	[554,432]	[41,105]	-5	hSpCas9	negative selection	4711089
104775698	104775720	14	-	27760321	HT1080	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	-1.9139796498390647	[347,271]	[27,37]	-6	hSpCas9	negative selection	4711090
104776665	104776687	14	+	27760321	HT29	viability after 7 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.48390368542845685	[212,165]	[398,291,338]	7	hSpCas9	negative selection	4794549
104776683	104776705	14	+	27760321	HT29	viability after 7 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.4441992011330784	[368,287]	[321,220,386]	-6	hSpCas9	negative selection	4794550
104773535	104773557	14	-	27760321	HT29	viability after 7 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.11959701495353392	[628,490]	[713,600,678]	-2	hSpCas9	negative selection	4794551
104774997	104775019	14	-	27760321	HT29	viability after 7 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.5475061548396065	[554,432]	[836,1053,880]	8	hSpCas9	negative selection	4794552
104775698	104775720	14	-	27760321	HT29	viability after 7 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.25650544437900336	[347,271]	[627,266,551]	4	hSpCas9	negative selection	4794553
104776665	104776687	14	+	27760321	HT29	viability after 25 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.4517192249930739	[212,165]	[292,326,264]	5	hSpCas9	negative selection	4878012
104776683	104776705	14	+	27760321	HT29	viability after 25 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.3630003539046197	[368,287]	[262,322,290]	-4	hSpCas9	negative selection	4878013
104773535	104773557	14	-	27760321	HT29	viability after 25 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.5447972826020291	[628,490]	[386,455,479]	-5	hSpCas9	negative selection	4878014
104774997	104775019	14	-	27760321	HT29	viability after 25 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.3490850431827059	[554,432]	[741,630,788]	4	hSpCas9	negative selection	4878015
104775698	104775720	14	-	27760321	HT29	viability after 25 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.46219027230593523	[347,271]	[411,536,522]	5	hSpCas9	negative selection	4878016
104776665	104776687	14	+	27760321	HT29	viability after 22 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.4905125535046899	[212,165]	[338,213,369]	5	hSpCas9	negative selection	4961475
104776683	104776705	14	+	27760321	HT29	viability after 22 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.04922507012971704	[368,287]	[432,323,420]	0	hSpCas9	negative selection	4961476
104773535	104773557	14	-	27760321	HT29	viability after 22 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.49839852967961373	[628,490]	[440,507,425]	-4	hSpCas9	negative selection	4961477
104774997	104775019	14	-	27760321	HT29	viability after 22 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.09851467136853481	[554,432]	[694,631,503]	1	hSpCas9	negative selection	4961478
104775698	104775720	14	-	27760321	HT29	viability after 22 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.08181615890715474	[347,271]	[536,288,306]	0	hSpCas9	negative selection	4961479
104776665	104776687	14	+	27760321	HT29	viability after 19 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.8112619749735865	[212,165]	[410,626,115]	8	hSpCas9	negative selection	5044938
104776683	104776705	14	+	27760321	HT29	viability after 19 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.20640223549585962	[368,287]	[456,207,629]	2	hSpCas9	negative selection	5044939
104773535	104773557	14	-	27760321	HT29	viability after 19 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.20203999495994412	[628,490]	[391,363,883]	-2	hSpCas9	negative selection	5044940
104774997	104775019	14	-	27760321	HT29	viability after 19 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.4249536662198559	[554,432]	[604,1059,599]	4	hSpCas9	negative selection	5044941
104775698	104775720	14	-	27760321	HT29	viability after 19 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.39332018550879094	[347,271]	[556,588,267]	4	hSpCas9	negative selection	5044942
104776665	104776687	14	+	27760321	HT29	viability after 16 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.6464055377966033	[212,165]	[381,299,318]	7	hSpCas9	negative selection	5128401
104776683	104776705	14	+	27760321	HT29	viability after 16 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.2272752671664207	[368,287]	[253,314,707]	2	hSpCas9	negative selection	5128402
104773535	104773557	14	-	27760321	HT29	viability after 16 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.03757160015948291	[628,490]	[669,641,523]	0	hSpCas9	negative selection	5128403
104774997	104775019	14	-	27760321	HT29	viability after 16 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.6394824462876723	[554,432]	[1260,802,557]	7	hSpCas9	negative selection	5128404
104775698	104775720	14	-	27760321	HT29	viability after 16 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.49723507852412807	[347,271]	[645,354,485]	6	hSpCas9	negative selection	5128405
104776665	104776687	14	+	27760321	HT29	viability after 13 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.7002659072524443	[212,165]	[412,261,393]	8	hSpCas9	negative selection	5211864
104776683	104776705	14	+	27760321	HT29	viability after 13 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.15466294965270852	[368,287]	[423,226,609]	2	hSpCas9	negative selection	5211865
104773535	104773557	14	-	27760321	HT29	viability after 13 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.3445271044799953	[628,490]	[417,637,480]	-4	hSpCas9	negative selection	5211866
104774997	104775019	14	-	27760321	HT29	viability after 13 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.2126080146637896	[554,432]	[846,349,786]	2	hSpCas9	negative selection	5211867
104775698	104775720	14	-	27760321	HT29	viability after 13 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	0.41008440847206473	[347,271]	[365,744,331]	5	hSpCas9	negative selection	5211868
104776665	104776687	14	+	27760321	HT29	viability after 10 days	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	0.58440913101086	[212,165]	[326,243,486]	7	hSpCas9	negative selection	5295327
104776683	104776705	14	+	27760321	HT29	viability after 10 days	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.41128830696931085	[368,287]	[400,182,336]	-5	hSpCas9	negative selection	5295328
104773535	104773557	14	-	27760321	HT29	viability after 10 days	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	-0.1548907425206722	[628,490]	[731,421,721]	-2	hSpCas9	negative selection	5295329
104774997	104775019	14	-	27760321	HT29	viability after 10 days	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.17387182050842015	[554,432]	[491,762,830]	2	hSpCas9	negative selection	5295330
104775698	104775720	14	-	27760321	HT29	viability after 10 days	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	-0.2737221538706236	[347,271]	[424,342,195]	-3	hSpCas9	negative selection	5295331
104776665	104776687	14	+	27760321	MOLM13	viability	TCAGGAGTCTCCACATGGAAGG	AKT1	ENSG00000142208	-1.3732715758033383	[290,212]	[35,69]	-7	hSpCas9	negative selection	5378790
104776683	104776705	14	+	27760321	MOLM13	viability	AAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.29243829013999606	[422,368]	[228,141]	-2	hSpCas9	negative selection	5378791
104773535	104773557	14	-	27760321	MOLM13	viability	CGTGTGTTCTCCGAGGACCGGG	AKT1	ENSG00000142208	0.030855783191470254	[586,628]	[384,324]	0	hSpCas9	negative selection	5378792
104774997	104775019	14	-	27760321	MOLM13	viability	AGCCCTGCTTTACAGGACGAGG	AKT1	ENSG00000142208	0.7016917348033878	[715,554]	[531,618]	5	hSpCas9	negative selection	5378793
104775698	104775720	14	-	27760321	MOLM13	viability	GCTCACCCAGTGACAACTCAGG	AKT1	ENSG00000142208	1.5441660624532196	[387,347]	[1037,235]	9	hSpCas9	negative selection	5378794
104795256	104795279	14	+	27661255	K562	viability	GGGCCGGCAGCCTGCACCCCCGG	AKT1	ENSG00000142208	0.13200609409482297	[784,961]	[858,678]	4	dCas9-KRAB	negative selection	5461695
104795673	104795696	14	+	27661255	K562	viability	GGCCGCCTGCTCCCGTCTTCGGG	AKT1	ENSG00000142208	-0.050726472721632554	[523,463]	[397,367]	-2	dCas9-KRAB	negative selection	5461696
104795336	104795359	14	-	27661255	K562	viability	GGAAGTGGCCGAGCGGGCCTGGG	AKT1	ENSG00000142208	0.10690922670282133	[1057,1227]	[1120,860]	4	dCas9-KRAB	negative selection	5461697
104795639	104795662	14	-	27661255	K562	viability	GCTGGGCACCGGGCACCGAGCGG	AKT1	ENSG00000142208	-0.19309660153777464	[716,740]	[595,434]	-6	dCas9-KRAB	negative selection	5461698
104795656	104795679	14	-	27661255	K562	viability	GCGGCCGAGCACCGAGCGCTGGG	AKT1	ENSG00000142208	0.19920524891311997	[156,108]	[152,96]	7	dCas9-KRAB	negative selection	5461699
104795675	104795698	14	-	27661255	K562	viability	GGCCCGAAGACGGGAGCAGGCGG	AKT1	ENSG00000142208	-0.09299935764810746	[1033,945]	[713,766]	-3	dCas9-KRAB	negative selection	5461700
104773272	104773295	14	+	27260156	BXPC3	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.13946177331832624	[498]	[417,634,642,618]	3	hSpCas9	negative selection	5527668
104780117	104780140	14	+	27260156	BXPC3	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.40781037095424555	[1338]	[981,963,1065,1237]	-7	hSpCas9	negative selection	5527669
104780115	104780138	14	-	27260156	BXPC3	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.3614713019660244	[531]	[359,508,513,338]	-7	hSpCas9	negative selection	5527670
104773349	104773372	14	+	27260156	BXPC3	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.2758857210495973	[336]	[333,257,275,290]	-6	hSpCas9	negative selection	5591950
104774952	104774975	14	+	27260156	BXPC3	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.1023254299573696	[281]	[276,277,299,236]	-2	hSpCas9	negative selection	5591951
104774953	104774976	14	-	27260156	BXPC3	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.4575875515331746	[462]	[323,333,341,419]	-7	hSpCas9	negative selection	5591952
104773272	104773295	14	+	27260156	A673	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.4820369304440332	[498]	[892,733,794,938]	9	hSpCas9	negative selection	5648919
104780117	104780140	14	+	27260156	A673	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	0.06860284344920053	[1338]	[1736,1634,1530,1855]	1	hSpCas9	negative selection	5648920
104780115	104780138	14	-	27260156	A673	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.07908791772779294	[531]	[776,539,657,741]	2	hSpCas9	negative selection	5648921
104773349	104773372	14	+	27260156	A673	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.12032544286515456	[336]	[334,411,276,464]	-3	hSpCas9	negative selection	5713201
104774952	104774975	14	+	27260156	A673	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.24867985470813725	[281]	[422,199,320,208]	-6	hSpCas9	negative selection	5713202
104774953	104774976	14	-	27260156	A673	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.12743391042085556	[462]	[669,579,521,667]	3	hSpCas9	negative selection	5713203
104773272	104773295	14	+	27260156	A375	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.695885636542678	[498]	[911,1089,940,859]	8	hSpCas9	negative selection	5770170
104780117	104780140	14	+	27260156	A375	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.3715469332918122	[1338]	[1283,1356,1289,951]	-5	hSpCas9	negative selection	5770171
104780115	104780138	14	-	27260156	A375	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.0876112384790384	[531]	[692,597,640,693]	1	hSpCas9	negative selection	5770172
104773349	104773372	14	+	27260156	A375	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.11868787871006559	[336]	[400,473,314,283]	-2	hSpCas9	negative selection	5834452
104774952	104774975	14	+	27260156	A375	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.35984550106483637	[281]	[219,372,195,252]	-5	hSpCas9	negative selection	5834453
104774953	104774976	14	-	27260156	A375	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.12025220421487418	[462]	[822,473,494,227]	-2	hSpCas9	negative selection	5834454
104773272	104773295	14	+	27260156	COLO741	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.6170286937956349	[498]	[891,1161,877]	9	hSpCas9	negative selection	5891421
104780117	104780140	14	+	27260156	COLO741	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.02715052686901842	[1338]	[1992,1845,1252]	0	hSpCas9	negative selection	5891422
104780115	104780138	14	-	27260156	COLO741	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.017464910770634057	[531]	[845,640,549]	0	hSpCas9	negative selection	5891423
104773349	104773372	14	+	27260156	COLO741	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.0904946925558896	[336]	[480,365,373]	-2	hSpCas9	negative selection	5955703
104774952	104774975	14	+	27260156	COLO741	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.20470031228722962	[281]	[417,508,324]	4	hSpCas9	negative selection	5955704
104774953	104774976	14	-	27260156	COLO741	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.02997057850493899	[462]	[700,615,508]	0	hSpCas9	negative selection	5955705
104773272	104773295	14	+	27260156	CAL120	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.585453592003382	[498]	[957,920,919]	8	hSpCas9	negative selection	6012672
104780117	104780140	14	+	27260156	CAL120	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.14800290532223048	[1338]	[1851,1313,1398]	-2	hSpCas9	negative selection	6012673
104780115	104780138	14	-	27260156	CAL120	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.24492125991157376	[531]	[1314,444,674]	3	hSpCas9	negative selection	6012674
104773349	104773372	14	+	27260156	CAL120	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.23499577901914226	[336]	[308,412,339]	-3	hSpCas9	negative selection	6076954
104774952	104774975	14	+	27260156	CAL120	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.11192668225619262	[281]	[393,324,419]	1	hSpCas9	negative selection	6076955
104774953	104774976	14	-	27260156	CAL120	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.11378296727921534	[462]	[869,573,465]	1	hSpCas9	negative selection	6076956
104773272	104773295	14	+	27260156	CADOES1	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.6099926398567037	[498]	[569,731,611,927]	8	hSpCas9	negative selection	6133923
104780117	104780140	14	+	27260156	CADOES1	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.3355346354631701	[1338]	[963,1091,955,933]	-5	hSpCas9	negative selection	6133924
104780115	104780138	14	-	27260156	CADOES1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.07505756908966317	[531]	[354,619,394,510]	-1	hSpCas9	negative selection	6133925
104773349	104773372	14	+	27260156	CADOES1	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.2101803782118396	[336]	[376,294,393,384]	3	hSpCas9	negative selection	6198205
104774952	104774975	14	+	27260156	CADOES1	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.0053027568433619	[281]	[251,202,279,319]	0	hSpCas9	negative selection	6198206
104774953	104774976	14	-	27260156	CADOES1	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.07520950139518505	[462]	[439,405,351,437]	-1	hSpCas9	negative selection	6198207
104773272	104773295	14	+	27260156	EWS502	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.2490991229844956	[498]	[483,489,798,772]	4	hSpCas9	negative selection	6255174
104780117	104780140	14	+	27260156	EWS502	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.5064492230689723	[1338]	[1036,1077,863,1000]	-7	hSpCas9	negative selection	6255175
104780115	104780138	14	-	27260156	EWS502	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.4044745646118104	[531]	[443,374,271,621]	-6	hSpCas9	negative selection	6255176
104773349	104773372	14	+	27260156	EWS502	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.4276578068254846	[336]	[267,286,268,232]	-6	hSpCas9	negative selection	6319456
104774952	104774975	14	+	27260156	EWS502	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.452134031911963	[281]	[199,245,183,244]	-6	hSpCas9	negative selection	6319457
104774953	104774976	14	-	27260156	EWS502	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.43117886319338766	[462]	[243,400,454,367]	-6	hSpCas9	negative selection	6319458
104773272	104773295	14	+	27260156	EW8	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.4091283435932923	[498]	[491,538,460,661]	7	hSpCas9	negative selection	6376425
104780117	104780140	14	+	27260156	EW8	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.05285749067825041	[1338]	[1392,1081,936,826]	-1	hSpCas9	negative selection	6376426
104780115	104780138	14	-	27260156	EW8	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.08878549052208529	[531]	[505,440,451,439]	1	hSpCas9	negative selection	6376427
104773349	104773372	14	+	27260156	EW8	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.3308953862080871	[336]	[322,189,172,200]	-6	hSpCas9	negative selection	6440707
104774952	104774975	14	+	27260156	EW8	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.1784020262118955	[281]	[291,224,211,315]	3	hSpCas9	negative selection	6440708
104774953	104774976	14	-	27260156	EW8	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.32157127217778747	[462]	[402,405,530,515]	6	hSpCas9	negative selection	6440709
104773272	104773295	14	+	27260156	CORL105	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.6623637509976857	[498]	[955,923,973,852]	9	hSpCas9	negative selection	6497676
104780117	104780140	14	+	27260156	CORL105	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.127706909586482	[1338]	[1444,1304,1497,1502]	-2	hSpCas9	negative selection	6497677
104780115	104780138	14	-	27260156	CORL105	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.18983912345114912	[531]	[860,606,755,631]	3	hSpCas9	negative selection	6497678
104773349	104773372	14	+	27260156	CORL105	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.23350817345684927	[336]	[273,275,499,316]	-4	hSpCas9	negative selection	6561958
104774952	104774975	14	+	27260156	CORL105	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.35682098524855566	[281]	[317,197,236,277]	-6	hSpCas9	negative selection	6561959
104774953	104774976	14	-	27260156	CORL105	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.14944557955504822	[462]	[676,598,591,537]	3	hSpCas9	negative selection	6561960
104773272	104773295	14	+	27260156	HS294T	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.49545262766109843	[498]	[219,256,286,339]	6	hSpCas9	negative selection	6618927
104780117	104780140	14	+	27260156	HS294T	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.11703585510976189	[1338]	[491,523,440,441]	-2	hSpCas9	negative selection	6618928
104780115	104780138	14	-	27260156	HS294T	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.43657771750667945	[531]	[139,130,172,167]	-6	hSpCas9	negative selection	6618929
104773349	104773372	14	+	27260156	HS294T	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.3757071654811911	[336]	[115,96,117,388]	5	hSpCas9	negative selection	6683209
104774952	104774975	14	+	27260156	HS294T	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.8602107955803854	[281]	[42,41,68,93]	-8	hSpCas9	negative selection	6683210
104774953	104774976	14	-	27260156	HS294T	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.21626297613085577	[462]	[125,98,233,162]	-3	hSpCas9	negative selection	6683211
104773272	104773295	14	+	27260156	HCC44	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.36346007959696264	[498]	[689,608,1089,589]	6	hSpCas9	negative selection	6740178
104780117	104780140	14	+	27260156	HCC44	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.7282112728887389	[1338]	[913,665,1447,753]	-8	hSpCas9	negative selection	6740179
104780115	104780138	14	-	27260156	HCC44	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.9242176018823807	[531]	[340,173,538,273]	-8	hSpCas9	negative selection	6740180
104773349	104773372	14	+	27260156	HCC44	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.3843537686855588	[336]	[250,252,357,315]	-5	hSpCas9	negative selection	6804460
104774952	104774975	14	+	27260156	HCC44	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.5738798269876962	[281]	[231,126,315,211]	-7	hSpCas9	negative selection	6804461
104774953	104774976	14	-	27260156	HCC44	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.6702914994344187	[462]	[375,173,365,426]	-7	hSpCas9	negative selection	6804462
104773272	104773295	14	+	27260156	G402	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.06637718869789247	[498]	[884,741,652,324]	0	hSpCas9	negative selection	6861429
104780117	104780140	14	+	27260156	G402	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.49927723366270205	[1338]	[1360,1134,985,1164]	-6	hSpCas9	negative selection	6861430
104780115	104780138	14	-	27260156	G402	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.42223279559352755	[531]	[424,735,467,320]	-5	hSpCas9	negative selection	6861431
104773349	104773372	14	+	27260156	G402	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.2831056734618391	[336]	[581,272,317,214]	-4	hSpCas9	negative selection	6925711
104774952	104774975	14	+	27260156	G402	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-1.0649922203532265	[281]	[93,177,150,221]	-8	hSpCas9	negative selection	6925712
104774953	104774976	14	-	27260156	G402	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.6087182195400027	[462]	[667,410,164,285]	-6	hSpCas9	negative selection	6925713
104773272	104773295	14	+	27260156	L33	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5072313981087392	[498]	[722,304,431,681]	8	hSpCas9	negative selection	6982680
104780117	104780140	14	+	27260156	L33	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.3920730750272867	[1338]	[892,497,638,959]	-7	hSpCas9	negative selection	6982681
104780115	104780138	14	-	27260156	L33	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.4292931570526664	[531]	[370,157,274,386]	-8	hSpCas9	negative selection	6982682
104773349	104773372	14	+	27260156	L33	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.3205622331117566	[336]	[323,96,148,279]	-6	hSpCas9	negative selection	7046962
104774952	104774975	14	+	27260156	L33	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.06349018501265435	[281]	[221,132,163,272]	-1	hSpCas9	negative selection	7046963
104774953	104774976	14	-	27260156	L33	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.10662772456826716	[462]	[505,210,328,449]	2	hSpCas9	negative selection	7046964
104773272	104773295	14	+	27260156	K562	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5867939622889135	[498]	[862,658,595,566]	7	hSpCas9	negative selection	7103931
104780117	104780140	14	+	27260156	K562	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.19540831517495016	[1338]	[602,1326,1524,709]	-2	hSpCas9	negative selection	7103932
104780115	104780138	14	-	27260156	K562	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.4387509184809523	[531]	[363,433,198,394]	-5	hSpCas9	negative selection	7103933
104773349	104773372	14	+	27260156	K562	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.01350126187789219	[336]	[421,295,140,330]	0	hSpCas9	negative selection	7168213
104774952	104774975	14	+	27260156	K562	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.09552051068580325	[281]	[411,300,110,140]	-1	hSpCas9	negative selection	7168214
104774953	104774976	14	-	27260156	K562	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.31434552606979266	[462]	[473,453,450,640]	4	hSpCas9	negative selection	7168215
104773272	104773295	14	+	27260156	HT29	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.36526173092695413	[498]	[862,999,699,603]	7	hSpCas9	negative selection	7225182
104780117	104780140	14	+	27260156	HT29	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.06401588778342582	[1338]	[1451,1681,1801,1313]	-1	hSpCas9	negative selection	7225183
104780115	104780138	14	-	27260156	HT29	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.16959550347416302	[531]	[629,972,696,629]	3	hSpCas9	negative selection	7225184
104773349	104773372	14	+	27260156	HT29	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.4098980099010934	[336]	[404,284,340,216]	-6	hSpCas9	negative selection	7289464
104774952	104774975	14	+	27260156	HT29	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.27279019526021797	[281]	[369,301,336,148]	-5	hSpCas9	negative selection	7289465
104774953	104774976	14	-	27260156	HT29	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.10430931621900426	[462]	[557,636,723,507]	2	hSpCas9	negative selection	7289466
104773272	104773295	14	+	27260156	MHHES1	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.6880361539339876	[498]	[1141,1081,810,667]	9	hSpCas9	negative selection	7346433
104780117	104780140	14	+	27260156	MHHES1	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.2060728181312046	[1338]	[1681,1495,1286,895]	-4	hSpCas9	negative selection	7346434
104780115	104780138	14	-	27260156	MHHES1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.2390124521805823	[531]	[700,694,898,552]	4	hSpCas9	negative selection	7346435
104773349	104773372	14	+	27260156	MHHES1	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.10215526024119359	[336]	[391,335,317,360]	-2	hSpCas9	negative selection	7410715
104774952	104774975	14	+	27260156	MHHES1	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.239501242777459	[281]	[335,224,337,192]	-4	hSpCas9	negative selection	7410716
104774953	104774976	14	-	27260156	MHHES1	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.17024498399521226	[462]	[850,534,528,471]	3	hSpCas9	negative selection	7410717
104773272	104773295	14	+	27260156	MEWO	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.4866252339131132	[498]	[738,587,651]	8	hSpCas9	negative selection	7467684
104780117	104780140	14	+	27260156	MEWO	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.30314576687504524	[1338]	[1057,1007,987]	-6	hSpCas9	negative selection	7467685
104780115	104780138	14	-	27260156	MEWO	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.09032212674784454	[531]	[511,478,419]	-2	hSpCas9	negative selection	7467686
104773349	104773372	14	+	27260156	MEWO	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.621375976713991	[336]	[205,206,201]	-8	hSpCas9	negative selection	7531966
104774952	104774975	14	+	27260156	MEWO	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.1949548489494608	[281]	[227,211,250]	-4	hSpCas9	negative selection	7531967
104774953	104774976	14	-	27260156	MEWO	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.11595758063295283	[462]	[401,499,296]	-3	hSpCas9	negative selection	7531968
104773272	104773295	14	+	27260156	LNCAPCLONEFGC	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	-0.2620866371571514	[498]	[356,368,434,355]	-4	hSpCas9	negative selection	7588935
104780117	104780140	14	+	27260156	LNCAPCLONEFGC	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-1.1501296760169146	[1338]	[429,567,683,524]	-9	hSpCas9	negative selection	7588936
104780115	104780138	14	-	27260156	LNCAPCLONEFGC	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-1.1626123162223148	[531]	[203,162,264,239]	-9	hSpCas9	negative selection	7588937
104773349	104773372	14	+	27260156	LNCAPCLONEFGC	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.09362532550058245	[336]	[205,370,345,225]	-1	hSpCas9	negative selection	7653217
104774952	104774975	14	+	27260156	LNCAPCLONEFGC	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.6395756866451986	[281]	[148,156,220,137]	-7	hSpCas9	negative selection	7653218
104774953	104774976	14	-	27260156	LNCAPCLONEFGC	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.6335329909903318	[462]	[326,235,260,256]	-7	hSpCas9	negative selection	7653219
104773272	104773295	14	+	27260156	PANC0327	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.55039715994721	[498]	[535,832,863,330]	7	hSpCas9	negative selection	7710186
104780117	104780140	14	+	27260156	PANC0327	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.05295782643893099	[1338]	[1358,1076,964,1210]	-1	hSpCas9	negative selection	7710187
104780115	104780138	14	-	27260156	PANC0327	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.2565858847159868	[531]	[514,729,661,325]	4	hSpCas9	negative selection	7710188
104773349	104773372	14	+	27260156	PANC0327	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.5332685452906716	[336]	[309,105,283,148]	-6	hSpCas9	negative selection	7774468
104774952	104774975	14	+	27260156	PANC0327	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.30495406013961224	[281]	[145,160,326,177]	-4	hSpCas9	negative selection	7774469
104774953	104774976	14	-	27260156	PANC0327	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.49152235986432524	[462]	[182,302,431,241]	-6	hSpCas9	negative selection	7774470
104773272	104773295	14	+	27260156	NCIH2009	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5032111938675147	[498]	[1138,911,942]	7	hSpCas9	negative selection	7831437
104780117	104780140	14	+	27260156	NCIH2009	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.17046002234527458	[1338]	[1973,1811,1256]	-2	hSpCas9	negative selection	7831438
104780115	104780138	14	-	27260156	NCIH2009	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.08319173540318675	[531]	[1123,534,772]	1	hSpCas9	negative selection	7831439
104773349	104773372	14	+	27260156	NCIH2009	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.2933823583887456	[336]	[432,416,310]	-4	hSpCas9	negative selection	7895719
104774952	104774975	14	+	27260156	NCIH2009	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.028384340791196894	[281]	[345,349,454]	0	hSpCas9	negative selection	7895720
104774953	104774976	14	-	27260156	NCIH2009	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.2691703284003994	[462]	[687,684,947]	3	hSpCas9	negative selection	7895721
104773272	104773295	14	+	27260156	NCIH1373	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	1.012893597161467	[498]	[1558,940,1000,287]	8	hSpCas9	negative selection	7952688
104780117	104780140	14	+	27260156	NCIH1373	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.07747514359516261	[1338]	[1446,1369,1635,320]	0	hSpCas9	negative selection	7952689
104780115	104780138	14	-	27260156	NCIH1373	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.16863826565885803	[531]	[397,444,699,147]	-2	hSpCas9	negative selection	7952690
104773349	104773372	14	+	27260156	NCIH1373	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.6244321769623637	[336]	[903,347,588,146]	6	hSpCas9	negative selection	8016970
104774952	104774975	14	+	27260156	NCIH1373	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-1.0260758039771152	[281]	[87,205,90,58]	-8	hSpCas9	negative selection	8016971
104774953	104774976	14	-	27260156	NCIH1373	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.06240075156309678	[462]	[805,277,422,145]	0	hSpCas9	negative selection	8016972
104773272	104773295	14	+	27260156	PATU8902	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.634137626813497	[498]	[1100,1699,829,694]	6	hSpCas9	negative selection	8073939
104780117	104780140	14	+	27260156	PATU8902	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.3364443847687588	[1338]	[2202,1284,1121,1352]	-3	hSpCas9	negative selection	8073940
104780115	104780138	14	-	27260156	PATU8902	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.019132516335981314	[531]	[596,795,779,827]	0	hSpCas9	negative selection	8073941
104773349	104773372	14	+	27260156	PATU8902	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.5173732577247872	[336]	[932,725,405,683]	5	hSpCas9	negative selection	8138221
104774952	104774975	14	+	27260156	PATU8902	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.6573972567733296	[281]	[400,114,342,88]	-6	hSpCas9	negative selection	8138222
104774953	104774976	14	-	27260156	PATU8902	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.07341575902138182	[462]	[941,714,469,625]	0	hSpCas9	negative selection	8138223
104773272	104773295	14	+	27260156	PANC1	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.4547367632988055	[498]	[701,598,420,611]	7	hSpCas9	negative selection	8195190
104780117	104780140	14	+	27260156	PANC1	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.010091596942264136	[1338]	[1293,1220,1055,1011]	0	hSpCas9	negative selection	8195191
104780115	104780138	14	-	27260156	PANC1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.002117573069178813	[531]	[395,671,399,407]	0	hSpCas9	negative selection	8195192
104773349	104773372	14	+	27260156	PANC1	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.1681055962535729	[336]	[197,377,291,194]	-3	hSpCas9	negative selection	8259472
104774952	104774975	14	+	27260156	PANC1	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.12377465206144334	[281]	[327,225,212,131]	-2	hSpCas9	negative selection	8259473
104774953	104774976	14	-	27260156	PANC1	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.18549694592319196	[462]	[302,435,471,222]	-3	hSpCas9	negative selection	8259474
104773272	104773295	14	+	27260156	PANC0813	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5278235745098924	[498]	[773,985,751,858]	8	hSpCas9	negative selection	8316441
104780117	104780140	14	+	27260156	PANC0813	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.2487127910680751	[1338]	[1218,1196,1297,1555]	-4	hSpCas9	negative selection	8316442
104780115	104780138	14	-	27260156	PANC0813	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.044923913875282245	[531]	[597,851,539,585]	0	hSpCas9	negative selection	8316443
104773349	104773372	14	+	27260156	PANC0813	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.2985005869551878	[336]	[341,431,241,274]	-5	hSpCas9	negative selection	8380723
104774952	104774975	14	+	27260156	PANC0813	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.3065830977077214	[281]	[177,221,442,222]	-5	hSpCas9	negative selection	8380724
104774953	104774976	14	-	27260156	PANC0813	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.03561296347925823	[462]	[718,602,358,460]	0	hSpCas9	negative selection	8380725
104773272	104773295	14	+	27260156	RDES	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.31504494001142186	[498]	[588,702,744,845]	5	hSpCas9	negative selection	8437692
104780117	104780140	14	+	27260156	RDES	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.34255332709015396	[1338]	[965,1133,1344,1483]	-5	hSpCas9	negative selection	8437693
104780115	104780138	14	-	27260156	RDES	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.020370164303472882	[531]	[501,542,830,629]	0	hSpCas9	negative selection	8437694
104773349	104773372	14	+	27260156	RDES	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.30086214110747633	[336]	[325,452,597,573]	5	hSpCas9	negative selection	8501974
104774952	104774975	14	+	27260156	RDES	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.39015704580249466	[281]	[222,253,246,264]	-6	hSpCas9	negative selection	8501975
104774953	104774976	14	-	27260156	RDES	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.17338846659794335	[462]	[359,537,457,546]	-3	hSpCas9	negative selection	8501976
104773272	104773295	14	+	27260156	PC3	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.26164369370003837	[498]	[364,668,675,751]	5	hSpCas9	negative selection	8558943
104780117	104780140	14	+	27260156	PC3	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.21562674992090614	[1338]	[723,1033,1966,1277]	-5	hSpCas9	negative selection	8558944
104780115	104780138	14	-	27260156	PC3	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.044212483481139125	[531]	[506,571,591,468]	1	hSpCas9	negative selection	8558945
104773349	104773372	14	+	27260156	PC3	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.27928454730473085	[336]	[146,252,442,367]	-6	hSpCas9	negative selection	8623225
104774952	104774975	14	+	27260156	PC3	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.6881176888109172	[281]	[143,178,194,178]	-9	hSpCas9	negative selection	8623226
104774953	104774976	14	-	27260156	PC3	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.23578417499691806	[462]	[283,317,544,492]	-5	hSpCas9	negative selection	8623227
104773272	104773295	14	+	27260156	PATU8988T	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.22652273736643364	[498]	[474,673,699,402]	4	hSpCas9	negative selection	8680194
104780117	104780140	14	+	27260156	PATU8988T	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.023743227380728094	[1338]	[1086,1146,1576,1262]	0	hSpCas9	negative selection	8680195
104780115	104780138	14	-	27260156	PATU8988T	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.020857406028548547	[531]	[751,444,471,400]	0	hSpCas9	negative selection	8680196
104773349	104773372	14	+	27260156	PATU8988T	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.3632628530818177	[336]	[190,342,216,271]	-6	hSpCas9	negative selection	8744476
104774952	104774975	14	+	27260156	PATU8988T	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.15020756755925135	[281]	[198,156,195,437]	-3	hSpCas9	negative selection	8744477
104774953	104774976	14	-	27260156	PATU8988T	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.609454249213339	[462]	[286,417,207,270]	-7	hSpCas9	negative selection	8744478
104773272	104773295	14	+	27260156	T47D	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	-0.3630864054964133	[498]	[422,388,458,544]	-7	hSpCas9	negative selection	8801445
104780117	104780140	14	+	27260156	T47D	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.843700987060146	[1338]	[726,826,965,960]	-9	hSpCas9	negative selection	8801446
104780115	104780138	14	-	27260156	T47D	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.9180231425901206	[531]	[323,319,327,340]	-9	hSpCas9	negative selection	8801447
104773349	104773372	14	+	27260156	T47D	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.07154138029055956	[336]	[333,343,374,445]	-2	hSpCas9	negative selection	8865727
104774952	104774975	14	+	27260156	T47D	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.6820353767859336	[281]	[167,160,253,239]	-8	hSpCas9	negative selection	8865728
104774953	104774976	14	-	27260156	T47D	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.34169924511206595	[462]	[366,300,433,616]	-6	hSpCas9	negative selection	8865729
104773272	104773295	14	+	27260156	SU8686	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.6587724073900356	[498]	[702,733,764,959]	9	hSpCas9	negative selection	8922696
104780117	104780140	14	+	27260156	SU8686	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.3193296372721457	[1338]	[898,1041,1078,1285]	-5	hSpCas9	negative selection	8922697
104780115	104780138	14	-	27260156	SU8686	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.19520548239212032	[531]	[653,541,510,722]	3	hSpCas9	negative selection	8922698
104773349	104773372	14	+	27260156	SU8686	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.33029910406811913	[336]	[289,247,170,364]	-5	hSpCas9	negative selection	8986978
104774952	104774975	14	+	27260156	SU8686	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.048752880353198136	[281]	[310,326,218,287]	0	hSpCas9	negative selection	8986979
104774953	104774976	14	-	27260156	SU8686	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.05829979234300604	[462]	[452,351,468,507]	-1	hSpCas9	negative selection	8986980
104773272	104773295	14	+	27260156	SKES1	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.5653529917740112	[498]	[766,719,894,575]	9	hSpCas9	negative selection	9043947
104780117	104780140	14	+	27260156	SKES1	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.13376379539249994	[1338]	[1255,1347,1173,1112]	-3	hSpCas9	negative selection	9043948
104780115	104780138	14	-	27260156	SKES1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.0773157311097666	[531]	[529,663,438,408]	-2	hSpCas9	negative selection	9043949
104773349	104773372	14	+	27260156	SKES1	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.24116867355426405	[336]	[504,427,291,381]	5	hSpCas9	negative selection	9108229
104774952	104774975	14	+	27260156	SKES1	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.2096181611889194	[281]	[342,181,225,227]	-4	hSpCas9	negative selection	9108230
104774953	104774976	14	-	27260156	SKES1	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.1749674252233466	[462]	[512,427,432,288]	-4	hSpCas9	negative selection	9108231
104773272	104773295	14	+	27260156	TOV112D	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.4223847295947948	[498]	[879,733,591,509]	7	hSpCas9	negative selection	9165198
104780117	104780140	14	+	27260156	TOV112D	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.15079656530415053	[1338]	[1296,1302,987,1270]	-3	hSpCas9	negative selection	9165199
104780115	104780138	14	-	27260156	TOV112D	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.32365158186641824	[531]	[446,1288,549,518]	6	hSpCas9	negative selection	9165200
104773349	104773372	14	+	27260156	TOV112D	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.10450621299398627	[336]	[475,288,449,230]	2	hSpCas9	negative selection	9229480
104774952	104774975	14	+	27260156	TOV112D	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.15898153018046557	[281]	[350,385,292,252]	3	hSpCas9	negative selection	9229481
104774953	104774976	14	-	27260156	TOV112D	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.020754505517987226	[462]	[369,717,393,403]	0	hSpCas9	negative selection	9229482
104773272	104773295	14	+	27260156	TC71	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.33131987185558	[498]	[678,605,590,827]	7	hSpCas9	negative selection	9286449
104780117	104780140	14	+	27260156	TC71	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.060869360314056875	[1338]	[1219,1439,1283,1578]	-1	hSpCas9	negative selection	9286450
104780115	104780138	14	-	27260156	TC71	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.05256011264688107	[531]	[592,491,473,647]	-1	hSpCas9	negative selection	9286451
104773349	104773372	14	+	27260156	TC71	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	0.06908229432260249	[336]	[350,297,414,455]	1	hSpCas9	negative selection	9350731
104774952	104774975	14	+	27260156	TC71	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	0.008474036157262055	[281]	[229,316,343,326]	0	hSpCas9	negative selection	9350732
104774953	104774976	14	-	27260156	TC71	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	0.03464382564220486	[462]	[418,543,548,522]	0	hSpCas9	negative selection	9350733
104773272	104773295	14	+	27260156	TC32	viability	TGTGCCGCAAAAGGTCTTCATGG	AKT1	ENSG00000142208	0.321491899304349	[498]	[535,479,638,715]	5	hSpCas9	negative selection	9407700
104780117	104780140	14	+	27260156	TC32	viability	TCACGTTGGTCCACATCCTGCGG	AKT1	ENSG00000142208	-0.2561386646031576	[1338]	[1121,671,1218,1318]	-5	hSpCas9	negative selection	9407701
104780115	104780138	14	-	27260156	TC32	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.11832648439991296	[531]	[474,330,478,586]	-2	hSpCas9	negative selection	9407702
104773349	104773372	14	+	27260156	TC32	viability	GCCCGTCCTTGTCCAGCATGAGG	AKT1	ENSG00000142208	-0.11663314795930269	[336]	[304,267,326,260]	-2	hSpCas9	negative selection	9471982
104774952	104774975	14	+	27260156	TC32	viability	GCCTGGAGTTCTGCAGGACGCGG	AKT1	ENSG00000142208	-0.388892190476091	[281]	[178,176,206,249]	-6	hSpCas9	negative selection	9471983
104774953	104774976	14	-	27260156	TC32	viability	ACCGCGTCCTGCAGAACTCCAGG	AKT1	ENSG00000142208	-0.02059245536498699	[462]	[576,289,492,369]	0	hSpCas9	negative selection	9471984
104774926	104774949	14	+	27869803	HPAFII	viability after 27 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.43752378171344053	[110]	[5,73,NaN]	-3	hSpCas9	negative selection	9550708
104775153	104775176	14	-	27869803	HPAFII	viability after 27 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.33798154353382825	[254]	[43,273,NaN]	2	hSpCas9	negative selection	9550709
104775705	104775728	14	+	27869803	HPAFII	viability after 27 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.2101078233177653	[273]	[413,265,NaN]	8	hSpCas9	negative selection	9550710
104776682	104776705	14	+	27869803	HPAFII	viability after 27 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.3510729517351504	[308]	[104,145,NaN]	-3	hSpCas9	negative selection	9550711
104780160	104780183	14	+	27869803	HPAFII	viability after 27 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.11328197401249673	[152]	[111,42,NaN]	-1	hSpCas9	negative selection	9550712
104792615	104792638	14	-	27869803	HPAFII	viability after 27 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.16057088824641774	[346]	[342,87,NaN]	1	hSpCas9	negative selection	9550713
104774926	104774949	14	+	27869803	HPAFII	viability after 15 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	0.04078408551943258	[110]	[110,129,NaN]	0	hSpCas9	negative selection	9633157
104775153	104775176	14	-	27869803	HPAFII	viability after 15 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.6429486789070498	[254]	[533,361,NaN]	7	hSpCas9	negative selection	9633158
104775705	104775728	14	+	27869803	HPAFII	viability after 15 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.0617770909989899	[273]	[988,381,NaN]	9	hSpCas9	negative selection	9633159
104776682	104776705	14	+	27869803	HPAFII	viability after 15 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.7160780047661073	[308]	[314,131,NaN]	-6	hSpCas9	negative selection	9633160
104780160	104780183	14	+	27869803	HPAFII	viability after 15 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.5300888358084943	[152]	[361,159,NaN]	6	hSpCas9	negative selection	9633161
104792615	104792638	14	-	27869803	HPAFII	viability after 15 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.1309518341050655	[346]	[572,305,NaN]	1	hSpCas9	negative selection	9633162
104774926	104774949	14	+	27869803	ASPC1	viability after 36 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	0.22210818522053655	[359]	[278,79,104]	2	hSpCas9	negative selection	9715606
104775153	104775176	14	-	27869803	ASPC1	viability after 36 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-0.3886752535835699	[763]	[258,147,206]	-4	hSpCas9	negative selection	9715607
104775705	104775728	14	+	27869803	ASPC1	viability after 36 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.0830119123935469	[621]	[811,274,354]	8	hSpCas9	negative selection	9715608
104776682	104776705	14	+	27869803	ASPC1	viability after 36 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.1457618906834172	[615]	[596,177,30]	1	hSpCas9	negative selection	9715609
104780160	104780183	14	+	27869803	ASPC1	viability after 36 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	0.31070790361533906	[585]	[102,488,169]	3	hSpCas9	negative selection	9715610
104792615	104792638	14	-	27869803	ASPC1	viability after 36 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-1.751135095685154	[767]	[131,114,13]	-9	hSpCas9	negative selection	9715611
104774926	104774949	14	+	27869803	PATU8988S	viability after 35 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-2.951119350268645	[210]	[5,15,NaN]	-9	hSpCas9	negative selection	9798055
104775153	104775176	14	-	27869803	PATU8988S	viability after 35 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-2.99659123708783	[593]	[18,40,NaN]	-9	hSpCas9	negative selection	9798056
104775705	104775728	14	+	27869803	PATU8988S	viability after 35 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	-0.4349460300678424	[811]	[203,279,NaN]	-4	hSpCas9	negative selection	9798057
104776682	104776705	14	+	27869803	PATU8988S	viability after 35 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-3.549925227823647	[727]	[39,9,NaN]	-9	hSpCas9	negative selection	9798058
104780160	104780183	14	+	27869803	PATU8988S	viability after 35 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-2.162758632725149	[433]	[54,22,NaN]	-9	hSpCas9	negative selection	9798059
104792615	104792638	14	-	27869803	PATU8988S	viability after 35 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-2.4714208192403038	[695]	[69,30,NaN]	-9	hSpCas9	negative selection	9798060
104774926	104774949	14	+	27869803	PATU8988S	viability after 31 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-1.7908458746685683	[210]	[0,53,NaN]	-9	hSpCas9	negative selection	9880504
104775153	104775176	14	-	27869803	PATU8988S	viability after 31 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	-2.147027139728921	[593]	[34,76,NaN]	-9	hSpCas9	negative selection	9880505
104775705	104775728	14	+	27869803	PATU8988S	viability after 31 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	-0.4635355432974565	[811]	[269,189,NaN]	-5	hSpCas9	negative selection	9880506
104776682	104776705	14	+	27869803	PATU8988S	viability after 31 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-2.400086700758931	[727]	[32,82,NaN]	-9	hSpCas9	negative selection	9880507
104780160	104780183	14	+	27869803	PATU8988S	viability after 31 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-2.514950647189397	[433]	[13,50,NaN]	-9	hSpCas9	negative selection	9880508
104792615	104792638	14	-	27869803	PATU8988S	viability after 31 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-2.5705443470759026	[695]	[40,53,NaN]	-9	hSpCas9	negative selection	9880509
104774926	104774949	14	+	27869803	HPAFII	viability after 35 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-1.7660336610794956	[110]	[0,33,NaN]	-7	hSpCas9	negative selection	9962953
104775153	104775176	14	-	27869803	HPAFII	viability after 35 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.5162780217966728	[254]	[43,342,NaN]	3	hSpCas9	negative selection	9962954
104775705	104775728	14	+	27869803	HPAFII	viability after 35 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.6587914524239862	[273]	[524,339,NaN]	9	hSpCas9	negative selection	9962955
104776682	104776705	14	+	27869803	HPAFII	viability after 35 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.7116769746789859	[308]	[71,121,NaN]	-4	hSpCas9	negative selection	9962956
104780160	104780183	14	+	27869803	HPAFII	viability after 35 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.7680687286307343	[152]	[68,18,NaN]	-4	hSpCas9	negative selection	9962957
104792615	104792638	14	-	27869803	HPAFII	viability after 35 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	0.024593599574870284	[346]	[260,85,NaN]	0	hSpCas9	negative selection	9962958
104774926	104774949	14	+	27869803	HPAFII	viability after 31 days	GCTCCCACTCACTGTGAGGAAGG	AKT1	ENSG00000142208	-0.807818377378978	[110]	[3,69,NaN]	-5	hSpCas9	negative selection	10045402
104775153	104775176	14	-	27869803	HPAFII	viability after 31 days	GGGCAAGGGCACTTTCGGCAAGG	AKT1	ENSG00000142208	0.3959069525432656	[254]	[60,326,NaN]	3	hSpCas9	negative selection	10045403
104775705	104775728	14	+	27869803	HPAFII	viability after 31 days	TGTCACTGGGTGAGCCCGACCGG	AKT1	ENSG00000142208	1.3973716356214732	[273]	[495,305,NaN]	8	hSpCas9	negative selection	10045404
104776682	104776705	14	+	27869803	HPAFII	viability after 31 days	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.46384114379082925	[308]	[63,192,NaN]	-3	hSpCas9	negative selection	10045405
104780160	104780183	14	+	27869803	HPAFII	viability after 31 days	AGGTGCCATCATTCTTGAGGAGG	AKT1	ENSG00000142208	-0.5420724045391064	[152]	[102,10,NaN]	-4	hSpCas9	negative selection	10045406
104792615	104792638	14	-	27869803	HPAFII	viability after 31 days	GACGTGGCTATTGTGAAGGAGGG	AKT1	ENSG00000142208	-0.2734800422345985	[346]	[252,60,NaN]	-2	hSpCas9	negative selection	10045407
104776682	104776705	14	+	28162770	P31FUJ	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.6135253391678877	[456]	[350]	-5	hSpCas9	negative selection	10109097
104775720	104775743	14	-	28162770	P31FUJ	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-2.1714016597502352	[68]	[17]	-8	hSpCas9	negative selection	10109098
104775132	104775155	14	-	28162770	P31FUJ	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.5255839536422244	[97]	[79]	-4	hSpCas9	negative selection	10109099
104770819	104770842	14	+	28162770	P31FUJ	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-2.200621798665229	[132]	[33]	-8	hSpCas9	negative selection	10109100
104780194	104780217	14	-	28162770	P31FUJ	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.8763924351062433	[338]	[216]	-6	hSpCas9	negative selection	10109101
104780127	104780150	14	-	28162770	P31FUJ	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.5357293373716852	[263]	[213]	-4	hSpCas9	negative selection	10109102
104780115	104780138	14	-	28162770	P31FUJ	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-1.1449294483890442	[413]	[219]	-7	hSpCas9	negative selection	10109103
104776722	104776745	14	+	28162770	P31FUJ	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.25262462275586284	[39]	[55]	2	hSpCas9	negative selection	10109104
104775768	104775791	14	-	28162770	P31FUJ	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.7085356353807764	[88]	[63]	-5	hSpCas9	negative selection	10109105
104775744	104775767	14	-	28162770	P31FUJ	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.7070985857726337	[438]	[315]	-5	hSpCas9	negative selection	10109106
104776682	104776705	14	+	28162770	TF1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	NaN	[NaN]	[356]	1	hSpCas9	negative selection	10278463
104775720	104775743	14	-	28162770	TF1	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	NaN	[NaN]	[10]	1	hSpCas9	negative selection	10278464
104775132	104775155	14	-	28162770	TF1	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	NaN	[NaN]	[32]	1	hSpCas9	negative selection	10278465
104770819	104770842	14	+	28162770	TF1	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	NaN	[NaN]	[19]	1	hSpCas9	negative selection	10278466
104780194	104780217	14	-	28162770	TF1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	NaN	[NaN]	[172]	1	hSpCas9	negative selection	10278467
104780127	104780150	14	-	28162770	TF1	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	NaN	[NaN]	[284]	1	hSpCas9	negative selection	10278468
104780115	104780138	14	-	28162770	TF1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	NaN	[NaN]	[313]	1	hSpCas9	negative selection	10278469
104776722	104776745	14	+	28162770	TF1	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	NaN	[NaN]	[26]	1	hSpCas9	negative selection	10278470
104775768	104775791	14	-	28162770	TF1	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	NaN	[NaN]	[67]	1	hSpCas9	negative selection	10278471
104775744	104775767	14	-	28162770	TF1	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	NaN	[NaN]	[280]	1	hSpCas9	negative selection	10278472
104776682	104776705	14	+	28162770	SKM1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.04250206001724521	[253]	[435]	0	hSpCas9	negative selection	10447829
104775720	104775743	14	-	28162770	SKM1	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.6068416007818689	[12]	[34]	6	hSpCas9	negative selection	10447830
104775132	104775155	14	-	28162770	SKM1	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-1.156420737092565	[28]	[22]	-7	hSpCas9	negative selection	10447831
104770819	104770842	14	+	28162770	SKM1	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.1361102884500685	[45]	[36]	-7	hSpCas9	negative selection	10447832
104780194	104780217	14	-	28162770	SKM1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.9475325801058645	[83]	[76]	-6	hSpCas9	negative selection	10447833
104780127	104780150	14	-	28162770	SKM1	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.16109034406755068	[73]	[116]	-1	hSpCas9	negative selection	10447834
104780115	104780138	14	-	28162770	SKM1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.24776560389390784	[89]	[133]	-2	hSpCas9	negative selection	10447835
104776722	104776745	14	+	28162770	SKM1	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	3.8314405405819176	[5]	[150]	9	hSpCas9	negative selection	10447836
104775768	104775791	14	-	28162770	SKM1	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.7239666710832869	[24]	[72]	7	hSpCas9	negative selection	10447837
104775744	104775767	14	-	28162770	SKM1	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.14961650060817205	[76]	[150]	1	hSpCas9	negative selection	10447838
104776682	104776705	14	+	28162770	SKM1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.04250206001724521	[253]	[435]	0	hSpCas9	negative selection	10617195
104775720	104775743	14	-	28162770	SKM1	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.6068416007818689	[12]	[34]	6	hSpCas9	negative selection	10617196
104775132	104775155	14	-	28162770	SKM1	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-1.156420737092565	[28]	[22]	-7	hSpCas9	negative selection	10617197
104770819	104770842	14	+	28162770	SKM1	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.1361102884500685	[45]	[36]	-7	hSpCas9	negative selection	10617198
104780194	104780217	14	-	28162770	SKM1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.9475325801058645	[83]	[76]	-6	hSpCas9	negative selection	10617199
104780127	104780150	14	-	28162770	SKM1	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.16109034406755068	[73]	[116]	-1	hSpCas9	negative selection	10617200
104780115	104780138	14	-	28162770	SKM1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.24776560389390784	[89]	[133]	-2	hSpCas9	negative selection	10617201
104776722	104776745	14	+	28162770	SKM1	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	3.8314405405819176	[5]	[150]	9	hSpCas9	negative selection	10617202
104775768	104775791	14	-	28162770	SKM1	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.7239666710832869	[24]	[72]	7	hSpCas9	negative selection	10617203
104775744	104775767	14	-	28162770	SKM1	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.14961650060817205	[76]	[150]	1	hSpCas9	negative selection	10617204
104776682	104776705	14	+	28162770	PL21	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.656773429563585	[843]	[232]	-4	hSpCas9	negative selection	10786561
104775720	104775743	14	-	28162770	PL21	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-0.9842849566481071	[49]	[10]	-6	hSpCas9	negative selection	10786562
104775132	104775155	14	-	28162770	PL21	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-1.6072153075682838	[118]	[16]	-8	hSpCas9	negative selection	10786563
104770819	104770842	14	+	28162770	PL21	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.1921778082894403	[167]	[31]	-7	hSpCas9	negative selection	10786564
104780194	104780217	14	-	28162770	PL21	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.030214613047007788	[296]	[131]	0	hSpCas9	negative selection	10786565
104780127	104780150	14	-	28162770	PL21	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-1.755313946557418	[286]	[36]	-8	hSpCas9	negative selection	10786566
104780115	104780138	14	-	28162770	PL21	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-1.0805658176715291	[344]	[70]	-6	hSpCas9	negative selection	10786567
104776722	104776745	14	+	28162770	PL21	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	2.074608732405461	[17]	[32]	9	hSpCas9	negative selection	10786568
104775768	104775791	14	-	28162770	PL21	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.15133275601205742	[73]	[28]	-1	hSpCas9	negative selection	10786569
104775744	104775767	14	-	28162770	PL21	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.3425946508425386	[302]	[103]	-2	hSpCas9	negative selection	10786570
104776682	104776705	14	+	28162770	P31FUJ	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.6135253391678877	[456]	[350]	-5	hSpCas9	negative selection	10955927
104775720	104775743	14	-	28162770	P31FUJ	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-2.1714016597502352	[68]	[17]	-8	hSpCas9	negative selection	10955928
104775132	104775155	14	-	28162770	P31FUJ	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.5255839536422244	[97]	[79]	-4	hSpCas9	negative selection	10955929
104770819	104770842	14	+	28162770	P31FUJ	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-2.200621798665229	[132]	[33]	-8	hSpCas9	negative selection	10955930
104780194	104780217	14	-	28162770	P31FUJ	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.8763924351062433	[338]	[216]	-6	hSpCas9	negative selection	10955931
104780127	104780150	14	-	28162770	P31FUJ	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.5357293373716852	[263]	[213]	-4	hSpCas9	negative selection	10955932
104780115	104780138	14	-	28162770	P31FUJ	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-1.1449294483890442	[413]	[219]	-7	hSpCas9	negative selection	10955933
104776722	104776745	14	+	28162770	P31FUJ	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.25262462275586284	[39]	[55]	2	hSpCas9	negative selection	10955934
104775768	104775791	14	-	28162770	P31FUJ	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.7085356353807764	[88]	[63]	-5	hSpCas9	negative selection	10955935
104775744	104775767	14	-	28162770	P31FUJ	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.7070985857726337	[438]	[315]	-5	hSpCas9	negative selection	10955936
104776682	104776705	14	+	28162770	OCIAML5	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.08894323844678009	[316]	[537]	0	hSpCas9	negative selection	11125293
104775720	104775743	14	-	28162770	OCIAML5	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	1.662506601965761	[27]	[159]	9	hSpCas9	negative selection	11125294
104775132	104775155	14	-	28162770	OCIAML5	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.22403534825095472	[43]	[67]	-2	hSpCas9	negative selection	11125295
104770819	104770842	14	+	28162770	OCIAML5	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-2.087694819357139	[72]	[30]	-8	hSpCas9	negative selection	11125296
104780194	104780217	14	-	28162770	OCIAML5	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.47569069195425806	[207]	[269]	-3	hSpCas9	negative selection	11125297
104780127	104780150	14	-	28162770	OCIAML5	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.01461958364995708	[182]	[326]	0	hSpCas9	negative selection	11125298
104780115	104780138	14	-	28162770	OCIAML5	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.07107635280579294	[212]	[365]	0	hSpCas9	negative selection	11125299
104776722	104776745	14	+	28162770	OCIAML5	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.03245621171606672	[12]	[23]	0	hSpCas9	negative selection	11125300
104775768	104775791	14	-	28162770	OCIAML5	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.35275322102450113	[58]	[135]	3	hSpCas9	negative selection	11125301
104775744	104775767	14	-	28162770	OCIAML5	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.35121702752474615	[227]	[524]	3	hSpCas9	negative selection	11125302
104776682	104776705	14	+	28162770	EOL1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.04533963653580697	[1611]	[822]	0	hSpCas9	negative selection	11294659
104775720	104775743	14	-	28162770	EOL1	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.21182972365100605	[58]	[35]	2	hSpCas9	negative selection	11294660
104775132	104775155	14	-	28162770	EOL1	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.5103892851460141	[218]	[80]	-4	hSpCas9	negative selection	11294661
104770819	104770842	14	+	28162770	EOL1	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-0.522338593747649	[228]	[83]	-4	hSpCas9	negative selection	11294662
104780194	104780217	14	-	28162770	EOL1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.33816738863118334	[508]	[338]	3	hSpCas9	negative selection	11294663
104780127	104780150	14	-	28162770	EOL1	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.6649039549375149	[509]	[425]	6	hSpCas9	negative selection	11294664
104780115	104780138	14	-	28162770	EOL1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.12866403461579215	[358]	[172]	-1	hSpCas9	negative selection	11294665
104776722	104776745	14	+	28162770	EOL1	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	-0.8828071504870696	[41]	[11]	-6	hSpCas9	negative selection	11294666
104775768	104775791	14	-	28162770	EOL1	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.6604147291506217	[137]	[45]	-5	hSpCas9	negative selection	11294667
104775744	104775767	14	-	28162770	EOL1	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.537811381055074	[386]	[295]	5	hSpCas9	negative selection	11294668
104776682	104776705	14	+	28162770	MOLM13	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.33611872046030955	[1305]	[1191]	2	hSpCas9	negative selection	11464025
104775720	104775743	14	-	28162770	MOLM13	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.6658287591564878	[67]	[77]	5	hSpCas9	negative selection	11464026
104775132	104775155	14	-	28162770	MOLM13	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.43900121406393977	[179]	[95]	-3	hSpCas9	negative selection	11464027
104770819	104770842	14	+	28162770	MOLM13	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-3.8935670775294207	[184]	[8]	-9	hSpCas9	negative selection	11464028
104780194	104780217	14	-	28162770	MOLM13	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.1790948457839619	[403]	[257]	-1	hSpCas9	negative selection	11464029
104780127	104780150	14	-	28162770	MOLM13	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	1.0556450971574152	[343]	[516]	7	hSpCas9	negative selection	11464030
104780115	104780138	14	-	28162770	MOLM13	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.7630208212335761	[452]	[192]	-4	hSpCas9	negative selection	11464031
104776722	104776745	14	+	28162770	MOLM13	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	-0.8540387133427836	[39]	[15]	-5	hSpCas9	negative selection	11464032
104775768	104775791	14	-	28162770	MOLM13	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.4788685971784419	[132]	[68]	-3	hSpCas9	negative selection	11464033
104775744	104775767	14	-	28162770	MOLM13	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.15068151181939704	[455]	[296]	-1	hSpCas9	negative selection	11464034
104776682	104776705	14	+	28162770	HEL	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.6939463279087934	[940]	[974]	6	hSpCas9	negative selection	11633391
104775720	104775743	14	-	28162770	HEL	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.20884230547019733	[76]	[56]	1	hSpCas9	negative selection	11633392
104775132	104775155	14	-	28162770	HEL	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	0.48459099833439817	[182]	[163]	4	hSpCas9	negative selection	11633393
104770819	104770842	14	+	28162770	HEL	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.880823124056656	[183]	[31]	-7	hSpCas9	negative selection	11633394
104780194	104780217	14	-	28162770	HEL	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	0.18330721336305966	[384]	[279]	1	hSpCas9	negative selection	11633395
104780127	104780150	14	-	28162770	HEL	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.6999637006933073	[345]	[359]	6	hSpCas9	negative selection	11633396
104780115	104780138	14	-	28162770	HEL	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.5938292315194105	[359]	[347]	5	hSpCas9	negative selection	11633397
104776722	104776745	14	+	28162770	HEL	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.8126638334426697	[31]	[35]	7	hSpCas9	negative selection	11633398
104775768	104775791	14	-	28162770	HEL	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.382675992697854	[96]	[80]	3	hSpCas9	negative selection	11633399
104775744	104775767	14	-	28162770	HEL	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.2180511626877939	[399]	[297]	1	hSpCas9	negative selection	11633400
104776682	104776705	14	+	28162770	MV411	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	-0.21482822600536422	[827]	[600]	-2	hSpCas9	negative selection	11802757
104775720	104775743	14	-	28162770	MV411	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.0035119678966859524	[44]	[37]	0	hSpCas9	negative selection	11802758
104775132	104775155	14	-	28162770	MV411	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.16759994849606863	[71]	[53]	-1	hSpCas9	negative selection	11802759
104770819	104770842	14	+	28162770	MV411	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-3.0055431903870957	[142]	[14]	-9	hSpCas9	negative selection	11802760
104780194	104780217	14	-	28162770	MV411	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.5001752875829393	[271]	[161]	-4	hSpCas9	negative selection	11802761
104780127	104780150	14	-	28162770	MV411	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.39644749991584166	[192]	[213]	3	hSpCas9	negative selection	11802762
104780115	104780138	14	-	28162770	MV411	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.6175908015865479	[252]	[326]	5	hSpCas9	negative selection	11802763
104776722	104776745	14	+	28162770	MV411	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.5693656456701377	[19]	[24]	5	hSpCas9	negative selection	11802764
104775768	104775791	14	-	28162770	MV411	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.24160052993984515	[79]	[56]	-2	hSpCas9	negative selection	11802765
104775744	104775767	14	-	28162770	MV411	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.3426995432931458	[292]	[312]	3	hSpCas9	negative selection	11802766
104776682	104776705	14	+	28162770	MONOMAC1	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.019072733466872993	[525]	[610]	0	hSpCas9	negative selection	11972123
104775720	104775743	14	-	28162770	MONOMAC1	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.9993603657439498	[23]	[54]	8	hSpCas9	negative selection	11972124
104775132	104775155	14	-	28162770	MONOMAC1	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-1.8838789617999236	[131]	[40]	-8	hSpCas9	negative selection	11972125
104770819	104770842	14	+	28162770	MONOMAC1	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.1181814263483143	[88]	[46]	-7	hSpCas9	negative selection	11972126
104780194	104780217	14	-	28162770	MONOMAC1	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.9291036418129207	[195]	[117]	-6	hSpCas9	negative selection	11972127
104780127	104780150	14	-	28162770	MONOMAC1	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-1.7740941496953637	[181]	[60]	-8	hSpCas9	negative selection	11972128
104780115	104780138	14	-	28162770	MONOMAC1	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-1.089054166186542	[192]	[103]	-7	hSpCas9	negative selection	11972129
104776722	104776745	14	+	28162770	MONOMAC1	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	0.1248912478278088	[15]	[19]	1	hSpCas9	negative selection	11972130
104775768	104775791	14	-	28162770	MONOMAC1	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.849113543639247	[43]	[27]	-6	hSpCas9	negative selection	11972131
104775744	104775767	14	-	28162770	MONOMAC1	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-1.4844265232424374	[226]	[92]	-8	hSpCas9	negative selection	11972132
104776682	104776705	14	+	28162770	OCIAML2	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.5763775346512754	[116]	[966]	5	hSpCas9	negative selection	12141489
104775720	104775743	14	-	28162770	OCIAML2	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	0.712591998813609	[11]	[108]	7	hSpCas9	negative selection	12141490
104775132	104775155	14	-	28162770	OCIAML2	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.238899539326377	[32]	[154]	-2	hSpCas9	negative selection	12141491
104770819	104770842	14	+	28162770	OCIAML2	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.2947799898427435	[26]	[60]	-7	hSpCas9	negative selection	12141492
104780194	104780217	14	-	28162770	OCIAML2	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.5171724111791915	[62]	[243]	-4	hSpCas9	negative selection	12141493
104780127	104780150	14	-	28162770	OCIAML2	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	0.7062479368419179	[45]	[415]	7	hSpCas9	negative selection	12141494
104780115	104780138	14	-	28162770	OCIAML2	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	0.23424213921419168	[49]	[325]	2	hSpCas9	negative selection	12141495
104776722	104776745	14	+	28162770	OCIAML2	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	-0.055592325963317535	[5]	[31]	0	hSpCas9	negative selection	12141496
104775768	104775791	14	-	28162770	OCIAML2	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	0.5187229305583302	[16]	[134]	5	hSpCas9	negative selection	12141497
104775744	104775767	14	-	28162770	OCIAML2	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	0.3571891993751587	[59]	[425]	3	hSpCas9	negative selection	12141498
104776682	104776705	14	+	28162770	OCIAML3	viability	GAAGGTGCGTTCGATGACAGTGG	AKT1	ENSG00000142208	0.5336256394017105	[199]	[873]	5	hSpCas9	negative selection	12310855
104775720	104775743	14	-	28162770	OCIAML3	viability	GGAGGAGATGGACTTCCGGTCGG	AKT1	ENSG00000142208	-3.1175695963811765	[22]	[7]	-9	hSpCas9	negative selection	12310856
104775132	104775155	14	-	28162770	OCIAML3	viability	GGTGATCCTGGTGAAGGAGAAGG	AKT1	ENSG00000142208	-0.8778006063247544	[27]	[45]	-6	hSpCas9	negative selection	12310857
104770819	104770842	14	+	28162770	OCIAML3	viability	GTGACCTGGGGCTTGAAGGGTGG	AKT1	ENSG00000142208	-1.594007640324163	[43]	[43]	-8	hSpCas9	negative selection	12310858
104780194	104780217	14	-	28162770	OCIAML3	viability	GGGAGTACATCAAGACCTGGCGG	AKT1	ENSG00000142208	-0.04236521280303346	[72]	[213]	0	hSpCas9	negative selection	12310859
104780127	104780150	14	-	28162770	OCIAML3	viability	CAAGGAGCGGCCGCAGGATGTGG	AKT1	ENSG00000142208	-0.3045010231291782	[62]	[153]	-2	hSpCas9	negative selection	12310860
104780115	104780138	14	-	28162770	OCIAML3	viability	GCAGGATGTGGACCAACGTGAGG	AKT1	ENSG00000142208	-0.877179691729033	[100]	[165]	-6	hSpCas9	negative selection	12310861
104776722	104776745	14	+	28162770	OCIAML3	viability	ATGATGAAGGTGTTGGGCCGGGG	AKT1	ENSG00000142208	2.3128829552843553	[7]	[119]	9	hSpCas9	negative selection	12310862
104775768	104775791	14	-	28162770	OCIAML3	viability	GACAACCGCCATCCAGACTGTGG	AKT1	ENSG00000142208	-0.8681826037631575	[25]	[42]	-6	hSpCas9	negative selection	12310863
104775744	104775767	14	-	28162770	OCIAML3	viability	TGACGGCCTCAAGAAGCAGGAGG	AKT1	ENSG00000142208	-0.16516434152028892	[103]	[279]	-1	hSpCas9	negative selection	12310864