RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 31157
  • Symbol: Ocrl
  • Description: Oculocerebrorenal syndrome of Lowe
DataSource: http://genomernai.dkfz.de/v16/genedetails/31157

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 31157 FBgn0023508 CG3573 DRSC18576 0.944920797 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 31157 FBgn0023508 CG3573 DRSC18576 0 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00014-A 31157 Ocrl DRSC18576 strong Decreased Flag-Mad nuclear accumulation no Dpp pathway regulation Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. Xu et al. 2007 17785517 Cell line S2R+ Flag-Mad protein expression and subcellular location Fluorescence rp Genome-wide dsRNA Visual inspection np
GR00031-A-1 31157 HFA18576 -1.1 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 31157 HFA18576 0.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00036-A 31157 CG3573 CG3573 DRSC18576 strong Decreased GFP protein expression yes decreased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00038-A-0 31157 FBgn0023508 CG3573 np **** Fewer infected cells, less vacuolar escape, fewer bacteria per cell no L. monocytogenes infection Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Cheng et al. 2005 16157870 Cell line S2 L. monocytogenes infection High content (microscopy) Custom-made dsRNA Visual inspection np
31157 FBgn0023508 CG3573 CT11908 sp none no library: PHOSPHO
31157 FBgn0023508 CG3573 LP11751 sp none no library: DGC1
GR00134-A-1 31157 CG3573 CG3573 34649 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 31157 CG3573 CG3573 34650 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 31157 CG3573 CG3573 34649 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 31157 CG3573 34649 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 31157 CG3573 CG3573 34649 -0.685714285714285 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 31157 CG3573 34649 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 31157 CG3573 34650 2 Gain of bristles no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00159-A 31157 CG3573 DRSC18576 np none no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00190-A-1 31157 FBgn0023508 CG3573 34649 -0.073 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00202-A 31157 FBgn0023508 CG3573 np sp none no Vaccinia virus infection A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. Moser et al. 2010 20585561 Cell line DL1 Virally encoded beta-galactosidase protein expression Fluorescence Custom-made Kinases, phosphatases and selected genes dsRNA Z-score < -2 in both duplicates Author-reviewed data
31157 FBgn0023508 CG3573 np (-2) - (-1.5) Decreased BiP signal peptide and firefly luciferase fusion protein expression no
GR00218-S 31157 CG3573 Ocrl AMB20863 0.07 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00282-A 31157 CG3573 CG3573 34649 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 31157 CG3573 CG3573 110796 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 31157 CG3573 Ocrl 34649 -1.3203680247548901 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 31157 CG3573 EG:86E4.5 np 2 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 31157 FBgn0023508 CG3573 DRSC18576 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00330-A 31157 CG3573 DRSC18576 np Decreased androgen receptor (AR) reporter expression Androgen receptor (AR) regulation A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells Imberg-Kazdan et al. 2013 23403032 Cell line S2R+ Androgen receptor (AR) reporter Luminescence Whole Genome Drosophila Library (DRSC 2.0) Genome-wide dsRNA Complex, sp Complex criteria Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’.
GR00335-A 31157 FBgn0023508 Ocrl HMS01201 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
31157 FBgn0023508 np np sp none
GR00367-S 31157 CG3573 34649 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 31157 CG3573 34649 -0.22889217377208665 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 31157 CG3573 GD34650 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31157 CG3573 GD34649 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31157 CG3573 BL34722 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.