RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 31254
  • Symbol: Lint-O
  • Description: L(3)mbt interactor in ovarian somatic cells
DataSource: http://genomernai.dkfz.de/v16/genedetails/31254

Export (tab separated) Export to Excel
Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 31254 FBgn0024993 CG2662 DRSC18468 -1.062576165 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 31254 FBgn0024993 CG2662 DRSC18468 0.023865054 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 31254 FBgn0024993 DRSC18468 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 31254 HFA18468 -0.8 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 31254 HFA18468 -0.8 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
31254 FBgn0024993 CG2662 RE68603 sp none no library: DGC2
GR00134-A-1 31254 CG2662 CG2662 33544 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 31254 CG2662 CG2662 33545 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 31254 CG2662 CG2662 33544 -0.47 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 31254 CG2662 33544 1 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-2 31254 CG2662 33544 2.37 none no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00142-A-1 31254 CG2662 CG2662 33544 -0.471428571428571 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 31254 CG2662 33544 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 31254 CG2662 33545 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00189-A-1 31254 FBgn0024993 CG2662 BKN30097 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 31254 FBgn0024993 CG2662 2662R-2 np none yes C96-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 31254 FBgn0024993 CG2662 2662R-2 sp Downregulation of Notch pathway; adult wing posterior compartment: crumbled, opaque; wing disc NRE:EGFP: down yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 31254 FBgn0024993 CG2662 2662R-1 sp Upregulation of Notch pathway; adult wing veins: disrupted, thinner; adult wing posterior compartment: crumbled, opaque yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 31254 FBgn0024993 CG2662 np np none no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 31254 FBgn0024993 CG2662 33544 -0.352 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 31254 FBgn0024993 CG2662 DRSC18468 1.08 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 31254 FBgn0024993 CG2662 DRSC18468 0.17 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 31254 FBgn0024993 CG2662 DRSC18468 0.29 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 31254 FBgn0024993 CG2662 DRSC18468 1.69 Increased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00217-A-1 31254 CG2662 CG2662 np medium Decreased WgRluc and not changed sFluc protein expression yes Wg pathway regulation (1) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Cell line S2R+ WgRluc and sFluc protein expression Luminescence np Genome-wide dsRNA Phenotype strength Complex criteria Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 31254 CG2662 CG2662 33544 0 none yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 31254 CG2662 CG2662 33545 0 none yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 31254 CG2662 CG2662 106361 np none yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 31254 CG2662 CG2662 106361 stereotypic phenotype Wings: unfolded yes Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 31254 CG2662 CG2662 33545 np none yes line lost Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 31254 CG2662 CG2662 33544 wildtype none yes Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 31254 CG2662 CG2662 106361 stereotypic phenotype Wings: round no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 31254 CG2662 CG2662 33544 wildtype none no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 31254 CG2662 CG2662 33545 wildtype none no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S 31254 CG2662 AMB18240 0.57 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 31254 CG2662 2662R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 31254 CG2662 CG2662 33544 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 31254 CG2662 CG2662 106361 0.11825670725903151 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 31254 FBgn0024993 CG2662 DRSC18468 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
31254 FBgn0024993 np np sp none
GR00367-S 31254 CG2662 33544 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 31254 CG2662 106361 -0.2130737139844059 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 31254 CG2662 GD33544 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.