RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 31461
• Symbol: CG15784
• Description: uncharacterized protein

DataSource: http://genomernai.dkfz.de/v16/genedetails/31461

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	31461	FBgn0029766	CG15784	DRSC18090	-3.017654847	Decreased lipid droplet area		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31461	FBgn0029766	CG15784	DRSC18090	-1.819470044	Decreased lipid droplet area		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	31461			HFA18090	0.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31461			HFA18090	-0.4	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00044-A	31461		CG15784	HFA18090	-2.6	Downregulation of JAK/STAT pathway		no		JAK/STAT pathway regulation	Identification of JAK/STAT signalling components by genome-wide RNA interference.	Mueller et al.	2005	16094372	Cell line	Kc167	JAK/STAT pathway reporter	Luminescence	HFA	Genome-wide	dsRNA	Z-score	> 2 OR < -2
GR00134-A-1	31461	CG15784	CG15784	29598	np	Lethal		yes	early pupal	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	31461	CG15784	CG15784	29599	np	Lethal		yes	late larval	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-2	31461	CG15784	CG15784	np	np	none		yes		Muscle morphogenesis and function (2)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	Custom-made	Selected genes	UAS-IR construct	rp	S19 > 0.5
GR00134-A-3	31461	CG15784	CG15784	29599	np	Missing muscles, undefined larval sarcomere morphology		no		Muscle morphogenesis and function (3)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	ZCL0663 protein expression	Fluorescence	np	Selected genes	UAS-IR construct	np	np
GR00135-A-1	31461	CG15784	CG15784	29599	-0.84	Lethal		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00142-A-1	31461	CG15784	CG15784	29598	0.176470588235294	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	31461	CG15784		29599	sp	Completely lethal (before pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	31461	CG15784		29598	10	Notum malformation death		no	partially lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00152-A	31461	CG15784	CG15784	np	-2.9401	Downregulation of Notch pathway after Notch stimulation		no	N(delta)ecn Notch stimulation; con-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	31461	CG15784	CG15784	np	-1.3689	none		no	N(delta)ecn Notch stimulation; m3-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	31461	CG15784	CG15784	np	-0.39947	none		no	without Notch stimulation	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00190-A-1	31461	FBgn0029766	CG15784	29598	-0.644	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00191-A-1	31461	CG15784	CG15784	np	np	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	31461	CG15784	CG15784	np	np	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-3	31461	CG15784	CG15784	np	7.5	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (3)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-4	31461	CG15784	CG15784	np	6	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (4)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-5	31461	CG15784	CG15784	np	0	none		yes		Blood cell homeostasis regulation (5)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	cg-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-6	31461	CG15784	CG15784	np	15.4	Increased melanotic mass formation		yes	confirmed melanotic suppressor gene, overlapping dsRNA	Blood cell homeostasis regulation (6)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-7	31461	CG15784	CG15784	np	5.5	Increased melanotic mass formation		yes	confirmed melanotic suppressor gene, overlapping dsRNA	Blood cell homeostasis regulation (7)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-8	31461	CG15784	CG15784	np	2.9	none		no	confirmed melanotic suppressor gene, overlapping dsRNA	Blood cell homeostasis regulation (8)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	cg-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00239-A-1	31461	CG15784		15784R	np	Lethal		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	31461	CG15784	CG15784	29599	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	31461	CG15784	CG15784	29598	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	31461	CG15784	CG15784	104583	0.06718883875495407	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00291-A	31461		CG15784	DRSC18090	sp	Increased or decreased hedgehog (hh) secretion			eliminated as hit by authors, sp	Hedgehog (hh) secretion regulation	A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila	Aikin et al.	2012	22432040	Cell line	S2	Hedgehog protein expression	Luminescence	DRSC	Genome-wide	dsRNA	Z-score	< -2 OR > 2 by at least 1/3 Z-scores	296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00311-A	31461	FBgn0029766	CG15784	DRSC18090	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	31461	CG15784	CG15784	104583	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00335-A	31461	FBgn0029766	CG15784	HMS00983	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	31461	FBgn0029766	np	np	sp	none
GR00367-S	31461	CG15784		29599	np	liquid clearance defect			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	31461	CG15784		104583	-0.1861821175359472	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	31461	CG15784		GD29599		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	31461	CG15784		BL34529		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	31461	CG15784		KK104583		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.