RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 318246
  • Symbol: CG32850
  • Description: uncharacterized protein
DataSource: http://genomernai.dkfz.de/v16/genedetails/318246

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 318246 FBgn0052850 CG32850 DRSC21574 -0.207926433 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 318246 FBgn0052850 CG32850 DRSC21574 -1.288980001 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00014-A 318246 PMCA DRSC17113 weak Decreased Flag-Mad nuclear accumulation no Dpp pathway regulation Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. Xu et al. 2007 17785517 Cell line S2R+ Flag-Mad protein expression and subcellular location Fluorescence rp Genome-wide dsRNA Visual inspection np
GR00031-A-1 318246 HFA17113 0.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 318246 HFA17113 0.1 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
318246 FBgn0027590, FBgn0052850 CG16936, CG32850 GH04753 sp none no library: DGC1
318246 FBgn0052850 CG32850 SD04061 sp none no library: DGC1
318246 FBgn0023524, FBgn0052850 CG3078, CG32850 GH07425 sp none no library: DGC1
318246 FBgn0052850 CG32850 RE36839 sp none no library: DGC2
318246 FBgn0052850 CG32850 RE41137 sp none no library: DGC2
318246 FBgn0052850, FBgn0010247 CG32850, Parp RH43209 sp none no library: DGC2
GR00159-A 318246 FBgn0025704 CG2165 DRSC17113, DRSC17114, DRSC17154, DRSC34373 severe Muscles rounded up yes Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00311-A 318246 FBgn0259214 PMCA DRSC17113 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00330-A 318246 CG32850 DRSC21574 np Decreased androgen receptor (AR) reporter expression Androgen receptor (AR) regulation A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells Imberg-Kazdan et al. 2013 23403032 Cell line S2R+ Androgen receptor (AR) reporter Luminescence Whole Genome Drosophila Library (DRSC 2.0) Genome-wide dsRNA Complex, sp Complex criteria Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’.
GR00335-A 318246 FBgn0052850 CG32850 JF01121 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
318246 FBgn0052850 np np sp none