RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 31907
• Symbol: Ptpmeg2
• Description: Protein tyrosine phosphatase Meg2

DataSource: http://genomernai.dkfz.de/v16/genedetails/31907

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18277	0.313807018	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18277	0.270017453	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18277	0.068114897	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18278	0.054025254	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18278	-0.638698426	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC18278	-0.916356737	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC23303	-0.703671673	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31907	FBgn0028341	l(1)G0232	DRSC23303	0.804774441	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00013-A	31907	CG32697	l(1)G0232	np	np	Decreased Chlamydia caviae infection		yes		Chlamydia caviae infection	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.	Derre et al.	2007	17967059	Cell line	SL2	Chlamydia MOMP and LPS expression and DNA content	Fluorescence	np	Genome-wide	dsRNA	Visual inspection	np	Additional information about secondary screens
GR00031-A-1	31907			HFA18277	0.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-1	31907			HFA18278	0.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31907			HFA18278	0.6	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31907			HFA18277	0.3	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	31907	FBgn0028341	l(1)G0232	CT7928	sp	none		no	library: PHOSPHO
	31907	FBgn0028341	l(1)G0232	LP08165	sp	none		no	library: DGC2
	31907	FBgn0028341	l(1)G0232	LD27988	sp	none		no	library: DGC1
	31907	FBgn0003129, FBgn0028341	Poxm, l(1)G0232	SD15443	sp	none		no	library: DGC2
GR00051-A-1	31907	CG32697	CG32697	np	np	Decreased Candida albicans phagocytosis		yes	validated (30 % cells phagocytosing)	Candida albicans phagocytosis (1)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	C. albicans phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing C. albicans	<
GR00051-A-2	31907	CG32697	CG32697	np	32	Decreased Escherichia coli phagocytosis		yes		Candida albicans phagocytosis (2)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	Escherichia coli phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing E. coli	<
GR00051-A-3	31907	CG32697	CG32697	np	35	Decreased latex beads phagocytosis		yes		Candida albicans phagocytosis (3)	Identification of Drosophila gene products required for phagocytosis of Candida albicans.	Stroschein-Stevenson et al.	2006	16336044	Cell line	S2	Latex beads phagocytosis, actin protein expression and DNA content	Fluorescence	rp	Selected genes	dsRNA	Percentage cells phagocytosing latex beads	<
GR00062-A	31907	FBgn0028341	l(1)G0232	np	2	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00062-A	31907	FBgn0028341	l(1)G0232	np	1	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00134-A-1	31907	CG32697	l(1)G0232	21276	np	Lethal		yes	late pupal	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	31907	CG32697	l(1)G0232	21275	np	none		yes		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-2	31907	CG32697	l(1)G0232	np	np	Wing posture / locomotion / ability to fly defect or lethal / semi-lethal		no		Muscle morphogenesis and function (2)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	Custom-made	Selected genes	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	31907	CG3101	l(1)G0232	21275	-0.14	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	31907	CG3101	l(1)G0232	21276	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	31907	CG3102	l(1)G0232	33744	-0.12	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	31907	CG32697		33744	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00138-A-1	31907	CG32697		21275	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00138-A-1	31907	CG32697		21276	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00144-A-4	31907	CG32697		33744	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	31907	CG32697		21275	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	31907	CG32697		21276	sp	Bristle morphology defects, notum malformation death		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00183-A	31907	FBgn0028341	l(1)G0232	21276	sp	GFP aggregates number, GFP aggregates size, neuroblast less large cells, ganglion mother cell shorter lineages, neuroblast loss, neuroblast loss with unchanged neuroblast cell size, neuroblast underproliferation, lethal		no		Self-renewal and differentiation in neural stem cells	Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi.	Neumueller et al.	2011	21549331	Tissue	insc-GAL4	Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability	Fluorescence	np	Genome-wide	UAS-IR construct	Phenotype strength	> 1	Additional information about a secondary screen (KK library)
GR00191-A-1	31907	CG32697	l(1)G0232	np	np	none		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	31907	CG32697	l(1)G0232	np	np	none		no		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00202-A	31907	FBgn0052697	I(1)G0232	np	sp	none		no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00216-A	31907	FBgn0028341	l(1)G0232	BKN28723	0.3	none		no		Wnt proteins secretion	p24 proteins are required for secretion of Wnt ligands.	Buechling et al.	2011	22094269	Cell line	S2R+	Wg pathway reporter	Luminescence	BKN	Cellular transporting machinery genes	dsRNA	Z-score	> 2.5 OR < -2.5	Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4)
GR00218-S	31907	CG32697		AMB20884	-1.84	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	31907	CG3101		3101R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	31907	CG32697	l(1)G0232	21275	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	31907	CG32697	l(1)G0232	33744	np	none			rudimentary ovaries	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	31907	CG32697	l(1)G0232	104427	0.3877293497025652	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	31907	np	np	DRSC18278	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A	31907	np	np	DRSC18277	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	31907	CG32697	l(1)G0232	104427	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	31907	FBgn0028341	l(1)G0232	104427	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	31907	FBgn0028341	l(1)G0232	HMS00731	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	31907	FBgn0028341	np	np	sp	none
GR00367-S	31907	CG32697		21276	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	31907	CG32697		104427	-0.15351090211620755	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	31907	CG32697		GD33744		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	31907	CG32697		GD21276		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.