Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 32153
Symbol: Upf1
Description: Upf1 RNA helicase

DataSource: http://genomernai.dkfz.de/v16/genedetails/32153

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	32153	FBgn0030354	Upf1	DRSC19667	3.037973574	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32153	FBgn0030354	Upf1	DRSC19667	1.120232533	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32153	FBgn0030354	Upf1	DRSC19667	1.889556449	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32153	FBgn0030354	Upf1	DRSC19667	-0.108793208	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	32153	FBgn0030354		DRSC19667	weak	ERK activation increased		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	32153			HFA19667	0.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	32153			HFA19667	0.7	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00045-A-0	32153	FBgn0030354		DRSC19667	7.41	Increased Hedgehog reporter activity		no		Hedgehog signaling	A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.	Nybakken et al.	2005	16311596	Cell line	Clone 8	Hedgehog signaling	Dual luciferase	DRSC		dsRNA	Z-score	>
	32153	FBgn0030354	Upf1	np	sp	none		no	library: DGC1
	32153	FBgn0030354	Upf1	LD30316	sp	none		no	library: DGC1
GR00088-A	32153	CG1559	Upf1	np	np	GFP protein expression under hypoxia				Translational activity under hypoxia	An RNA interference screen identifies a novel regulator of target of rapamycin that mediates hypoxia suppression of translation in Drosophila S2 cells.	Lee et al.	2008	18653470	Cell line	S2	GFP protein expression	Western blot	Custom-made	Selected genes	dsRNA	np	np
GR00152-A	32153	CG1559	Upf1	np	-1.0885	none		no	without Notch stimulation	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	32153	CG1559	Upf1	np	-2.8777	Downregulation of Notch pathway after Notch stimulation		no	N(delta)ecn Notch stimulation; con-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00152-A	32153	CG1559	Upf1	np	-0.26203	none		no	N(delta)ecn Notch stimulation; m3-luc normalization	Notch induced transcription	Modifiers of notch transcriptional activity identified by genome-wide RNAi.	Mourikis et al.	2010	20959007	Cell line	Kc167	Notch pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	< -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc)	Additional information about secondary screens
GR00175-A	32153	CG1559	Upf1	DRSC32645	0.148953679118707	none		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00175-A	32153	CG1559	Upf1	DRSC32646	-0.116294339372865	none		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00189-A-1	32153	FBgn0030354	Upf1	BKN27828	np	Downregulation of Notch pathway		no		Notch pathway regulation (1)	A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.	Saj et al.	2010	20493818	Cell line	S2	Notch pathway reporter	Luminescence	BKN	Genome-wide	dsRNA	Complex, sp	Complex criteria
GR00214-A-1	32153	FBgn0030354	Upf1	DRSC19667	-1.35	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	32153	FBgn0030354	Upf1	DRSC19667	-0.82	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32153	FBgn0030354	Upf1	DRSC19667	0.72	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32153	FBgn0030354	Upf1	DRSC19667	0.21	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	32153	CG1559		AMB22421	0.24	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00245-A	32153	FBgn0030354	Upf1	DRSC19667	np	Increased formation of mutant Huntingtin (Httex1-Qn) protein aggregates		yes	validated	Huntingtin (Htt) aggregates formation	A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila.	Zhang et al.	2010	20100940	Cell line	S2	Mutant Httex1-Qn-eGFP protein expression and DNA content	Fluorescence	Version 1 and np	Genome-wide	dsRNA	Aggregate number, size, and signal intensity	> 2 standard deviations	Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00311-A	32153	FBgn0030354	Upf1	DRSC19667	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
	32153	FBgn0030354	np	np	sp	none