Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 33798
Symbol: CG7236
Description: uncharacterized protein

DataSource: http://genomernai.dkfz.de/v16/genedetails/33798

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	33798	FBgn0031730	CG7236	DRSC03037	-1.458528849	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	33798	FBgn0031730	CG7236	DRSC03037	1.531254714	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00014-A	33798		CG7236	DRSC03037	strong	Decreased Flag-Mad nuclear accumulation		no		Dpp pathway regulation	Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads.	Xu et al.	2007	17785517	Cell line	S2R+	Flag-Mad protein expression and subcellular location	Fluorescence	rp	Genome-wide	dsRNA	Visual inspection	np
GR00026-A-0	33798	FBgn0031730	CG7236	np	np	Morphology		no		Cell morphology	A functional genomic analysis of cell morphology using RNA interference.	Kiger et al.	2003	14527345	Cell line	Kc167, S2R+	Morphology	High content (microscopy)	Custom-made		dsRNA	Visual inspection	np
GR00030-A-1	33798	CG7236	CG7236	CG7236	sp	Decreased mitotic index, multinucleate cells		yes		Cell cycle regulation (1)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content	Flow cytometry	rp	Kinases	dsRNA	Confidence intervals	Complex criteria
GR00030-A-2	33798	CG7236	CG7236	CG7236	np	none		no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	33798			HFA03037	-0.7	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	33798			HFA03037	0.8	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00032-A-0	33798	FBgn0037473		63189	np	Multi-nucleate cells		no		Cytokinesis	Terminal cytokinesis events uncovered after an RNAi screen.	Echard et al.	2004	15380073	Cell line	S2	Multinucleate cell incidence	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
	33798	FBgn0031730	CG7236	DRSC03037	weak	Binucleate cells		no
	33798	FBgn0031730	CG7236	CT22313	sp	none		no	library: PHOSPHO
GR00060-A-0	33798	FBgn0031730	CG7236	np	np	Abolished ER retention of cSpitz		no		EGFR signaling	Small wing PLCgamma is required for ER retention of cleaved Spitz during eye development in Drosophila.	Schlesinger et al.	2004	15469842	Cell line	S2R+	Spitz-GFP localization	High content (microscopy)	Custom-made library		dsRNA	Visual inspection	np
GR00148-A	33798	FBgn0031730	CG7236	np	1.99	Increased P-JNK protein expression with PGN	15 min PGN induction	no		PGN-induced dJNK phosphorylation	A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.	Bond and Foley	2009	19893628	Cell line	S2	P-JNK protein expression	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	Complex criteria	Only hits stored in GenomeRNAi
GR00201-A-1	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	Altered cell morphology		yes		Actin organization and cell morphology (1)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-2	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	Altered cell morphology		yes		Actin organization and cell morphology (2)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2R+	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-3	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	Altered cell morphology		yes		Actin organization and cell morphology (3)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	Kc167	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-4	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	none		yes		Actin organization and cell morphology (4)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG2-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-5	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	none		yes		Actin organization and cell morphology (5)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c1	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-6	33798	FBgn0031730	CG7236	CG7236 (1) (Baum_Lab), CG7236 (2) (Baum_Lab), CG7236 (UCSF)	np	none		yes		Actin organization and cell morphology (6)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00202-A	33798	FBgn0031730	CG7236	np	sp	none		no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00218-S	33798	CG7236		AMB21882	-2.17	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00311-A	33798	FBgn0031730	CG7236	DRSC03037	np	Other cellular phenotype			increased cell size, increased number of multinucleate cells	Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	33798	FBgn0031730	CG7236	JF02655	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	33798	FBgn0031730	np	np	sp	none
GR00370-A	33798	FBgn0031730	CG7236		-0.07	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	33798	FBgn0031730	CG7236		-0.56	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	33798	FBgn0031730	CG7236		1.24	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00388-A	33798	CG7236		BL27505		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.