RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 34780
• Symbol: p38b
• Description: p38b MAP kinase

DataSource: http://genomernai.dkfz.de/v16/genedetails/34780

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	34780	FBgn0024846	p38b	DRSC03586	-1.725390958	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	34780	FBgn0024846	p38b	DRSC03586	-0.316807697	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00030-A-1	34780	CG7393	p38b	p38b	np	none		yes		Cell cycle regulation (1)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content	Flow cytometry	rp	Kinases	dsRNA	Confidence intervals	Complex criteria
GR00030-A-2	34780	CG7393	p38b	p38b	np	none		no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	34780			HFA03586	0.3	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	34780			HFA03586	0.2	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	34780	FBgn0024846	p38b	CT22735	sp	none		no	library: PHOSPHO
	34780	FBgn0024846	p38b	GM01004	sp	none		no	library: DGC1
	34780	FBgn0024846	p38b	LD24658	sp	none		no	library: DGC1
GR00185-A	34780	CG7393	p38	np	71	none		no	20 min heat shock	Pol II elongation	Spt6 enhances the elongation rate of RNA polymerase II in vivo.	Ardehali et al.	2009	19279664	Cell line	S2	Hsp70 mRNA expression	Real-time qPCR	Custom-made	Transcription factors	dsRNA	Percentage	<
GR00191-A-1	34780	CG7393	p38b	np	np	none		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	34780	CG7393	p38b	np	np	none		yes		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-3	34780	CG7393	p38b	np	0	none		yes		Blood cell homeostasis regulation (3)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-4	34780	CG7393	p38b	np	0	none		yes		Blood cell homeostasis regulation (4)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-5	34780	CG7393	p38b	np	4	none		no		Blood cell homeostasis regulation (5)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	cg-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00201-A-1	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		yes		Actin organization and cell morphology (1)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-2	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		yes		Actin organization and cell morphology (2)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2R+	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-3	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		yes		Actin organization and cell morphology (3)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	Kc167	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-4	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		yes		Actin organization and cell morphology (4)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG2-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-5	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		yes		Actin organization and cell morphology (5)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c1	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-6	34780	FBgn0024846	p38b	CG7393 (UCSF)	np	none		no		Actin organization and cell morphology (6)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00202-A	34780	FBgn0024846	p38b	np	sp	none		no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00218-S	34780	CG7393	p38b	AMB30579	-0.73	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	34780	CG7393		7393R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	34780	CG7393	p38b	108099	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	34780	CG7393	p38b	108099	0.24099261631203053	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	34780	FBgn0024846	p38b	DRSC03586	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00326-A	34780	FBgn0024846	p38b	108099	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	34780	FBgn0024846	p38b	JF03341	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	34780	FBgn0024846	p38b	GL00140	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	34780	FBgn0024846	np	np	sp	none
GR00367-S	34780	CG7393		73931	np	none			NIG	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00370-A	34780	FBgn0024846	p38b		0.89	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	34780	FBgn0024846	p38b		-1.01	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	34780	FBgn0024846	p38b		-0.58	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00379-A-1	34780	CG7393		108099	-0.21826817486694577	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	34780	CG7393		BL29405		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	34780	CG7393		KK108099		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.