RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 34903
• Symbol: esg
• Description: escargot

DataSource: http://genomernai.dkfz.de/v16/genedetails/34903

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	34903	FBgn0001981	esg	DRSC03530	0.904697014	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	34903	FBgn0001981	esg	DRSC03530	-1.590524757	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	34903			HFA03530	-1.5	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	34903			HFA03530	0.5	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	34903	FBgn0001981	esg	RE64266	sp	none		no	library: DGC2
GR00065-A	34903	CG3758	esg	esg	np	none		no		Dendrite pattern formation	Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites.	Parrish et al.	2006	16547170	Tissue	GAL4221	mCD8 protein expression	Fluorescence	Custom-made	Transcription factors	UAS-IR construct	np	Phenotypes in multiple blind tests	Additional information about secondary screens
GR00082-A	34903		Esg	HFA03530	np	Increased SUMO-dependent transcriptional repression		no		SUMO-dependent transcriptional repression	Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen.	Stielow et al.	2008	18374648	Cell line	Kc167	SUMOylation pathway reporter	Luminescence	rp	Genome-wide	dsRNA	FLuc/Rluc ratio	Complex criteria	Additional information about secondary screens
GR00134-A-1	34903	CG3758	esg	9794	np	Flightless		yes		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	34903	CG3758	esg	9793	np	Lethal		yes	late pupal	Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-4	34903	CG3758	esg	9794	np	none		no		Muscle morphogenesis and function (4)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	F-actin protein expression	Fluorescence	np	Selected genes	UAS-IR construct	np	np
GR00135-A-1	34903	CG3758	esg	9793	-1.17	Developmentally lethal		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	34903	CG3758	esg	9794	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	34903	CG3758		9793	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00139-A	34903		esg	9793	sp	Lethal		no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00139-A	34903		esg	9794	sp	Lethal		no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1	34903	CG3758	esg	9793	0.466666666666666	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	34903	CG3758		9793	sp	Completely lethal (pupal)		no		Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	34903	CG3758		9794	sp	Gain of bristles, notum malformation death		no	partially lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	34903	FBgn0001981	esg	9793	0.161	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00218-S	34903	CG3758	esg	AMB19141	0.65	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	34903	CG3758		3758R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	34903	CG3758	esg	9794	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	34903	CG3758	esg	9793	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00311-A	34903	FBgn0001981	esg	DRSC03530	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	34903	FBgn0001981	esg	JF03134	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	34903	FBgn0001981	esg	HMS00025	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	34903	FBgn0001981	np	np	0.29	none			fold change: 0.8
GR00367-S	34903	CG3758		9793	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00388-A	34903	CG3758		BL34063		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	34903	CG3758		BL28514		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	34903	CG3758		GD9794		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.