| GR00002-A |
35002 |
FBgn0032598 |
CG17905 |
DRSC02640 |
-0.137788985 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
35002 |
FBgn0032598 |
CG17905 |
DRSC02640 |
-0.098340191 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00031-A-1 |
35002 |
|
|
HFA02640 |
0.7 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
35002 |
|
|
HFA02640 |
0.1 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
35002 |
FBgn0032598 |
CG17905 |
DRSC02640 |
weak |
Binucleate cells |
|
no |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
35002 |
FBgn0032598 |
CG17905 |
RE01566 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00062-A |
35002 |
FBgn0032598 |
CG17905 |
np |
3 |
Decreased Ca2+ influx |
|
|
|
Ca2+ channel regulation |
CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. |
Vig et al. |
2006 |
16645049 |
Cell line |
S2R+ |
Ca2+ influx |
Fluorescence |
np |
Genome-wide |
dsRNA |
Complex, sp |
> |
|
GR00218-S |
35002 |
CG17905 |
|
AMB33839 |
-1.38 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00282-A |
35002 |
CG17905 |
ChLD3 |
110501 |
np |
none |
|
|
not expressed |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
35002 |
CG17905 |
ChLD3 |
110501 |
-0.05211326723509124 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00311-A |
35002 |
FBgn0032598 |
ChLD3 |
DRSC02640 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
|
35002 |
FBgn0032598 |
np |
np |
1.06 |
none |
|
|
fold change: 0.58 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00379-A-1 |
35002 |
CG17905 |
|
110501 |
-0.13648138758496425 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00385-A |
35002 |
|
|
110501 |
0.5053921272525924 |
none |
|
|
|
Regulation of olfactory memory formation |
Identification of genes that promote or inhibit olfactory memory formation in Drosophila. |
Walkinshaw et al. |
2015 |
25644700 |
Organism |
Nsyb-GAL4 |
T-maze performance |
Visual inspection |
VDRC |
Selected genes |
UAS-IR construct |
Performance index, sp |
High performer in olfactory memory formation: > |
Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. |