RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 37123
  • Symbol: Prp19
  • Description: Pre-RNA processing factor 19
DataSource: http://genomernai.dkfz.de/v16/genedetails/37123

Export (tab separated) Export to Excel
Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 37123 FBgn0013969 Gbp DRSC07434 0.278722275 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 37123 FBgn0013969 Gbp DRSC07434 -4,00E-06 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00005-A-0 37123 FBgn0013969 DRSC07434 weak Centrosome clustering defects no Multipolar division Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. Kwon et al. 2008 18662975 Cell line S2 Centrosome clustering High content (microscopy) DRSC dsRNA Visual inspection np
GR00031-A-1 37123 HFA07434 0.4 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 37123 HFA07434 0.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00047-A-1 37123 FBgn0013969 Gbp DRSC07434 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
37123 FBgn0013969 Gbp LD02793 sp none no library: DGC1
GR00092-A 37123 FBgn0013969 CG5519 RDM1190-7301750 np none no Microtubule assembly and organization Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Ducat et al. 2008 18463163 Cell line S2 DNA content, alpha-tubulin and CP309 protein expression Fluorescence OpenBiosystems Drosophila RNAi Library 1.0 Mitotic regulator genes dsRNA Complex, sp Complex criteria
GR00131-A-1 37123 Gbp DRSC23905 25 - 50 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 37123 CG5519 Gbp 22147 np Lethal yes late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 37123 CG5519 Gbp 41438 np Lethal yes late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 37123 CG5519 Gbp 22146 np none yes Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-3 37123 CG5519 Gbp 22147, 41438 np none no Muscle morphogenesis and function (3) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 ZCL0663 protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 37123 CG5519 Gbp 22146 -0.45 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 37123 CG5519 Gbp 22147 2.47 Increased noxious heat avoidance yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 37123 CG5519 Gbp 41438 1.45 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 37123 CG5519 22146 1 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-2 37123 CG5519 22146 2.74 none no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00142-A-1 37123 CG5519 Gbp 22146 0.422222222222222 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 37123 CG5519 22147 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 37123 CG5519 41438 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 37123 CG5519 22146 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00170-A-1 37123 Gbp DRSC07434 -1.9 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 37123 Gbp DRSC34548 62.7 Decreased HIF dependent transcription with desferrioxamine no group B HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 37123 Gbp DRSC34547 51 Decreased HIF dependent transcription with desferrioxamine no group B HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00190-A-1 37123 FBgn0013969 Gbp 22146 -0.131 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00191-A-1 37123 CG5519 Prp19 np np Increased melanotic mass formation yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 37123 CG5519 Prp19 np np none yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 37123 CG5519 Prp19 np 9.3 Increased melanotic mass formation yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 37123 CG5519 Prp19 np 0 none yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 37123 CG5519 Prp19 np 0 none yes Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-6 37123 CG5519 Prp19 np 35.3 Increased melanotic mass formation yes confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (6) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-7 37123 CG5519 Prp19 np 0 none yes confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (7) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-8 37123 CG5519 Prp19 np 22.7 Increased melanotic mass formation no confirmed melanotic suppressor gene, non-overlapping dsRNA Blood cell homeostasis regulation (8) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00218-S 37123 CG5519 Prp19 AMB18106 -0.76 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 37123 CG5519 5519R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00245-A 37123 FBgn0013969 Gbp DRSC07434 np Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates yes validated Huntingtin (Htt) aggregates formation A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. Zhang et al. 2010 20100940 Cell line S2 Mutant Httex1-Qn-eGFP protein expression and DNA content Fluorescence Version 1 and np Genome-wide dsRNA Aggregate number, size, and signal intensity > 2 standard deviations Additional information about secondary screens (reproducibility and non-overlaping dsRNAs)
GR00282-A 37123 CG5519 Prp19 22146 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 37123 CG5519 Prp19 22147 np none distorted ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 37123 CG5519 Prp19 108575 -0.9294562182800757 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 37123 FBgn0261119 Prp19 DRSC07434 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00314-A 37123 FBgn0261119 Prp19 RNA002191 56.37 Decreased MAPK phosphorylation hit in validation screens RAS-related MAPK activation A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila Ashton-Beaucage et al. 2014 24643257 Cell line S2 MAPK phosphorylation Fluorescence Custom-made Genome-wide dsRNA pMAPK signal (%GFP dsRNA) np S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
GR00335-A 37123 FBgn0261119 Prp19 HMS00652 np Agametic Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
37123 np np HFA07434 -1.37 none fold change: 2.82
37123 FBgn0261119 np np sp none
GR00367-S 37123 CG5519 108575 np liquid clearance defect VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 37123 CG5519 108575 1.2967646495452199 Low decrease in nos and yTub expression No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 37123 CG5519 108575 np No germline, 100% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 37123 CG5519 BL32865 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 37123 CG5519 GD41438 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.