Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 37247
Symbol: hpo
Description: hippo

DataSource: http://genomernai.dkfz.de/v16/genedetails/37247

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	37247	FBgn0034453	hpo	DRSC06065	3.509845324	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	37247	FBgn0034453	hpo	DRSC06065	-0.17054883	none	no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00030-A-1	37247	CG11228	hippo	CG11228, CG11228_2	sp	Spindle defects, lagging chromatids	yes		Cell cycle regulation (1)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content	Flow cytometry	rp	Kinases	dsRNA	Confidence intervals	Complex criteria
GR00030-A-2	37247	CG11228	CG11228	CG11221, CG11228_2	np	none	no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	37247			HFA06065	-0.1	none	yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	37247			HFA06065	-0.3	none	no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	37247	FBgn0034453	hpo	CT31338	sp	none	no	library: PHOSPHO
	37247	FBgn0034453	hpo	np	sp	none	no	library: DGC1
	37247	FBgn0034453	hpo	GH10354	sp	none	no	library: DGC1
GR00089-A	37247		CG11228	np	0.02	Decreased dFoxO reporter expression			FoxO regulation	RNAi screening for kinases and phosphatases identifies FoxO regulators	Mattila et al.	2008	18815370	Cell line	S2	dFoxO reporter expression	Fluorescence	Custom-made	Kinases and phosphatases	dsRNA	P-value	<	Genome RNAi mapping in column after comment (FBgn)
GR00134-A-1	37247	CG11228	hpo	7823	np	none	no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00138-A-1	37247	CG11228		7823	1	none	no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCÎ”4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00139-A	37247		CG11228	7823	sp	Semi-lethal	no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1	37247	CG11228	hpo	7823	0.234567901234567	none	no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	37247	CG11228		7823	sp	Colour defects, overproliferation	no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	37247	FBgn0034453	hpo	7823	-0.097	none	no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00201-A-1	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	yes		Actin organization and cell morphology (1)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-2	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	yes		Actin organization and cell morphology (2)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2R+	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-3	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	yes		Actin organization and cell morphology (3)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	Kc167	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-4	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	yes		Actin organization and cell morphology (4)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG2-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-5	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	yes		Actin organization and cell morphology (5)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c1	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-6	37247	FBgn0034453	hpo	CG11228 (UCSF)	np	none	no		Actin organization and cell morphology (6)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00202-A	37247	FBgn0034453	hpo	np	sp	none	no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00218-S	37247	CG11228	hpo	AMB27604	-0.41	none	no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	37247	CG11228	hpo	7823	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	37247	CG11228	hpo	104169	np	none			piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	37247	CG11228	hpo	104169	0.4784702834503391	none			piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	37247	FBgn0261456	hpo	DRSC06065	np	none			Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	37247	CG11228	hpo	104169	np	none			Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	37247	FBgn0261456	hpo	104169	np	none			Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
GR00335-A	37247	FBgn0261456	hpo	GL00046	np	none			Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	37247	FBgn0261456	hpo	HMS00006	np	Embryonic lethal		many embryonic lethal	Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	37247	FBgn0261456	hpo	JF02740	np	none			Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	37247	FBgn0261456	np	np	sp	none
GR00367-S	37247	CG11228		104169	np	lethal or adult morphology phenotype		VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00370-A	37247	FBgn0034453	hpo		0.42	none			Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	37247	FBgn0034453	hpo		0.7	none			Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	37247	FBgn0034453	hpo		0.09	none			Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00379-A-1	37247	CG11228		104169	-0.4141047174307724	none		No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	37247	CG11228		BL27661		Lethal		ISC Overproliferation	Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	37247	CG11228		GD7823		none			Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A	37247	CG11228		BL33614		Lethal		ISC Overproliferation	Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.