RNAi

Gene Info

• Species: Fly (Drosophila melanogaster)
• GeneID: 37935
• Symbol: CG4707
• Description: uncharacterized protein

DataSource: http://genomernai.dkfz.de/v16/genedetails/37935

Export (tab separated) Export to Excel

Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	37935	FBgn0035036	CG4707	DRSC04483	-0.404882792	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	37935	FBgn0035036	CG4707	DRSC04483	-0.564750146	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	37935			HFA04483	-0.7	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	37935			HFA04483	-1.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	37935	FBgn0035036	CG4707	LD29879	sp	none		no	library: DGC1
GR00065-A	37935	CG4707	CG4707	CG4707	np	none		no		Dendrite pattern formation	Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites.	Parrish et al.	2006	16547170	Tissue	GAL4221	mCD8 protein expression	Fluorescence	Custom-made	Transcription factors	UAS-IR construct	np	Phenotypes in multiple blind tests	Additional information about secondary screens
GR00134-A-1	37935	CG4707	CG4707	26606	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	37935	CG4707	CG4707	26608	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	37935	CG4707	CG4707	26606	-0.13	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1	37935	CG4707	CG4707	26608	0.34	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00137-A-1	37935	CG4707	CG4707	np	3.1	Increased dVMAT protein cell surface expression		yes		Regulated secretory pathway biogenesis (1)	RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.	Asensio et al.	2010	21149569	Cell line	S2	dVMAT protein cell surface expression	Flow cytometry	DmRNAi library version 1	Selected genes	dsRNA	Z-score	>
GR00137-A-2	37935	CG4707	CG4707	np	np	Increased dVMAT protein cell surface expression		yes	no effect on DE/AA dVMAT protein cell surface expression	Regulated secretory pathway biogenesis (2)	RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.	Asensio et al.	2010	21149569	Cell line	S2	dVMAT protein cell surface expression	Flow cytometry	Custom-made	Selected genes	dsRNA	Cumulative frequency distribution of fluorescence ratios	p-value < 0.0025 in at least 2 of 4 independent experiments
GR00142-A-1	37935	CG4707	CG4707	26608	-0.0869565217391304	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	37935	CG4707		26606	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	37935	CG4707		26608	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	37935	FBgn0035036	CG4707	26608	0.034	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00191-A-1	37935	CG4707	CG4707	np	np	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	37935	CG4707	CG4707	np	np	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-3	37935	CG4707	CG4707	np	0	none		yes		Blood cell homeostasis regulation (3)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-4	37935	CG4707	CG4707	np	1.2	none		yes		Blood cell homeostasis regulation (4)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-5	37935	CG4707	CG4707	np	9.7	Increased melanotic mass formation		yes		Blood cell homeostasis regulation (5)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	cg-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-6	37935	CG4707	CG4707	np	0	none		yes	overlapping dsRNA	Blood cell homeostasis regulation (6)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-7	37935	CG4707	CG4707	np	1.8	none		yes	overlapping dsRNA	Blood cell homeostasis regulation (7)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00191-A-8	37935	CG4707	CG4707	np	0	none		no	overlapping dsRNA	Blood cell homeostasis regulation (8)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	cg-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	Tumor index	>
GR00218-S	37935	CG4707		AMB22797	-0.76	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	37935	CG4707		4707R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	37935	CG4707	CG4707	26606	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	37935	CG4707	CG4707	26606	-0.4127702739849486	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	37935	FBgn0035036	CG4707	DRSC04483	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
	37935	FBgn0035036	np	np	sp	none
GR00367-S	37935	CG4707		26606	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	37935	CG4707		26606	-0.379816335074325	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	37935	CG4707		GD26606		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.