RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 38321
  • Symbol: dos
  • Description: daughter of sevenless
DataSource: http://genomernai.dkfz.de/v16/genedetails/38321

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 38321 FBgn0016794 dos DRSC07992 0.211761658 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38321 FBgn0016794 dos DRSC07992 1.517947787 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38321 FBgn0016794 dos DRSC08713 0.67256477 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38321 FBgn0016794 dos DRSC08713 -1.046096364 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38321 FBgn0016794 dos DRSC23285 -3.004904384 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38321 FBgn0016794 dos DRSC23285 -1.525367164 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 38321 FBgn0016794 DRSC07992 strong ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 38321 HFA08713 0.4 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-1 38321 HFA07992 3.3 Decreased cell number and viability yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 38321 HFA07992 2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 38321 HFA08713 -0.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00043-A-0 38321 FBgn0016794 dos np np Increased IMD reporter activity no Innate immunity Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway. Kleino et al. 2005 16163390 Cell line S2 IMD/Rel signaling Dual luciferase Custom-made dsRNA Percentage IMD reporter activity >
GR00047-A-1 38321 FBgn0016794 dos DRSC07992 sp Decreased horseradish peroxidase protein expression yes Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
GR00047-A-2 38321 FBgn0016794 dos np np none no Constitutive protein secretion and Golgi organization (2) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Mouse Mannosidase II protein expression Fluorescence Selected genes dsRNA Visual inspection Golgi membrane depletion > 50 % of the cells
38321 FBgn0016794 dos SD02517 sp none no library: DGC1
GR00131-A-1 38321 dos DRSC07992 25 - 50 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00152-A 38321 CG1044 dos np 1.4936 none no without Notch stimulation Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 38321 CG1044 dos np 3.4008 Upregulation of Notch pathway after Notch stimulation no N(delta)ecn Notch stimulation; con-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 38321 CG1044 dos np 0.24341 none no N(delta)ecn Notch stimulation; m3-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00170-A-1 38321 dos DRSC07992 -4.9 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00189-A-1 38321 FBgn0016794 dos BKN28630 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 38321 FBgn0016794 dos 1044R-3 sp Up- and downregulation of Notch pathway; adult wing posterior compartment: crumbled; wing disc size: overgrown; wing disc NRE:EGFP: up, down, broader, gap; wing disc myrRFP: accumulation; lethal yes en-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 38321 FBgn0016794 dos 1044R-3 sp Up- and downregulation of Notch pathway; wing disc NRE:EGFP: up, gap yes en-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 38321 FBgn0016794 dos np np Upregulation of Notch pathway no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00214-A-1 38321 FBgn0016794 dos DRSC07992 -3.35 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 38321 FBgn0016794 dos DRSC07992 -2.2 Decreased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38321 FBgn0016794 dos DRSC07992 -6.15 Decreased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38321 FBgn0016794 dos DRSC07992 -6.73 Decreased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation yes RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 38321 CG1044 dos AMB17504 -0.39 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 38321 CG1044 1044R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 38321 CG1044 dos 101358 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 38321 CG1044 dos 101358 -1.1539497937242986 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00291-A 38321 dos DRSC07992 sp Increased or decreased hedgehog (hh) secretion eliminated as hit by authors, sp Hedgehog (hh) secretion regulation A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila Aikin et al. 2012 22432040 Cell line S2 Hedgehog protein expression Luminescence DRSC Genome-wide dsRNA Z-score < -2 OR > 2 by at least 1/3 Z-scores 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00311-A 38321 FBgn0016794 dos DRSC08713 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A 38321 FBgn0016794 dos DRSC07992 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 38321 CG1044 dos 101358 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00335-A 38321 FBgn0016794 dos HM04078 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
38321 FBgn0016794 np np sp none
GR00367-S 38321 CG1044 101358 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 38321 CG1044 101358 -0.33286465900144446 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 38321 CG1044 KK101358 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.