| GR00002-A |
38958 |
FBgn0010431 |
mtrm |
DRSC10322 |
2.028864841 |
Increased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
38958 |
FBgn0010431 |
mtrm |
DRSC10322 |
7.691379797 |
Increased lipid droplet area |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
38958 |
FBgn0010431 |
mtrm |
DRSC23076 |
-0.347278719 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
38958 |
FBgn0010431 |
mtrm |
DRSC23076 |
0.241087893 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00031-A-1 |
38958 |
|
|
HFA10322 |
0.4 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
38958 |
|
|
HFA10322 |
2.2 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
38958 |
FBgn0010431 |
mtrm |
LD47919 |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00128-A-1 |
38958 |
FBgn0010431 |
CG18543 |
DRSC10322 |
sp |
none |
|
no |
hit according to not so stringent primary analysis |
Combinatorial effect with methyl methanesulphonate (1) |
A network of conserved damage survival pathways revealed by a genomic RNAi screen. |
Ravi et al. |
2009 |
19543366 |
Cell line |
Kc167 |
Viability (synthetic lethal) |
Luminescence |
Version 1 |
Genome-wide |
dsRNA |
rp |
rp |
Additional information about the primary screen |
GR00128-A-2 |
38958 |
FBgn0010431 |
CG18543 |
DRSC35876 |
sp |
none |
|
no |
|
Combinatorial effect with methyl methanesulphonate (2) |
A network of conserved damage survival pathways revealed by a genomic RNAi screen. |
Ravi et al. |
2009 |
19543366 |
Cell line |
Kc167 |
Viability (synthetic lethal) |
Luminescence |
Version 1 |
Selected genes |
dsRNA |
rp |
rp |
|
GR00135-A-1 |
38958 |
CG18543 |
mtrm |
44904 |
0.24 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
38958 |
CG18543 |
Smr |
6441 |
0 |
Lethal |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
38958 |
CG18543 |
Smr |
44904 |
0.24 |
none |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00135-A-1 |
38958 |
CG18543 |
mtrm |
6441 |
0 |
Lethal |
|
yes |
|
Heat nociception (1) |
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. |
Neely et al. |
2010 |
21074052 |
Organism |
elav-GAL4 |
Noxious heat avoidance and viability |
Fly count |
np |
Genome-wide |
UAS-IR construct |
Z-score |
> 1.65 |
Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) |
GR00142-A-1 |
38958 |
CG18543 |
mtrm |
44904 |
-0.348484848484848 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00142-A-1 |
38958 |
CG18543 |
mtrm |
44904 |
-0.196581196581196 |
none |
|
no |
|
Serratia marcescens infection (1) |
Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. |
Cronin et al. |
2009 |
19520911 |
Organism |
HSP70-GAL4; TubGAL80ts |
Heat shock and viability |
Fly count |
np |
Random genes |
UAS-IR construct |
Days life time (LT50) |
< -1.5 SD OR > 2 SD |
|
GR00144-A-4 |
38958 |
CG18543, CG4013 |
|
6441 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00144-A-4 |
38958 |
CG18543, CG4013 |
|
44904 |
sp |
Completely lethal (pupal) |
|
no |
|
Notch pathway regulation (4) |
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. |
Mummery-Widmer et al. |
2009 |
19363474 |
Tissue |
pnr-GAL4 |
External sensory organ morphology and viability |
Visual inspection |
np |
Genome-wide |
UAS-IR construct |
Phenotype strength |
np |
|
GR00159-A |
38958 |
|
mtrm |
DRSC10322 |
np |
none |
|
no |
|
Muscle assembly and maintenance |
RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. |
Bai et al. |
2008 |
18359903 |
Primary cells |
primary embryonic cells |
Muscle cell morphology |
Fluorescence |
np |
Selected and random genes |
dsRNA |
Percentage of muscles with a given phenotype |
Severe: > 80 %; medium: ~ 50 % |
|
GR00218-S |
38958 |
CG18543 |
mtrm |
AMB29110 |
-0.75 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
38958 |
CG18543 |
|
18543R |
np |
Lethal |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00282-A |
38958 |
CG18543 |
mtrm |
44904 |
np |
none |
|
|
not expressed |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00282-A |
38958 |
CG18543 |
mtrm |
107579 |
np |
none |
|
|
not expressed |
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
38958 |
CG18543 |
mtrm |
107579 |
-0.34141336926900007 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00311-A |
38958 |
FBgn0010431 |
mtrm |
DRSC10322 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00315-A-1 |
38958 |
CG18543 |
mtrm |
107579 |
np |
Effect on follicular epithelium morphology |
|
|
|
Follicular epithelium development (1) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Random genes |
UAS-IR construct |
Visual inspection |
np |
Further classification screen available, see follicular epithelium development (2). |
GR00315-A-2 |
38958 |
CG18543 |
mtrm |
107579 |
np |
none |
|
|
|
Follicular epithelium development (2) |
A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche |
Berns et al. |
2014 |
24762813 |
Tissue |
traffic jam-GAL4 |
Follicular epithelium morphology |
Fluorescence |
VDRC KK |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. |
GR00335-A |
38958 |
FBgn0010431 |
mtrm |
GL00354 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
38958 |
FBgn0010431 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
38958 |
CG18543 |
|
107579 |
np |
none |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00379-A-1 |
38958 |
CG18543 |
|
107579 |
-0.2959687143324205 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
38958 |
CG18543 |
|
GD44904 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |