RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 39088
  • Symbol: RpS17
  • Description: Ribosomal protein S17
DataSource: http://genomernai.dkfz.de/v16/genedetails/39088

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 39088 FBgn0005533 RpS17 DRSC11271 1.418692401 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 39088 FBgn0005533 RpS17 DRSC11271 4.803533225 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 39088 HFA11271 1.9 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 39088 HFA11271 2.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00036-A 39088 CG3922 RpS17 np moderate Increased GFP protein expression no increased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00039-A-0 39088 FBgn0005533 DRSC11271 np Drosophila C virus infection reduced no Drosophila C virus infection Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. Cherry et al. 2005 15713840 Cell line S2 Drosophila C virus infection High content (microscopy) DRSC dsRNA Visual inspection > 40% reduced infection
GR00045-A-0 39088 FBgn0005533 DRSC11271 -3.17 Decreased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
GR00047-A-1 39088 FBgn0005533 RpS17 DRSC11271 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
GR00062-A 39088 FBgn0005533 RpS17 np 2 Decreased Ca2+ influx Ca2+ channel regulation CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Vig et al. 2006 16645049 Cell line S2R+ Ca2+ influx Fluorescence np Genome-wide dsRNA Complex, sp >
GR00090-A-0 39088 FBgn0005533 CG3922 np strong (but ribosomal) More dispersed droplets no Lipid storage Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Guo et al. 2008 18408709 Cell line S2 Lipid droplet staining High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00092-A 39088 FBgn0005533 CG3922 RDM1190-7302472 np none no Microtubule assembly and organization Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Ducat et al. 2008 18463163 Cell line S2 DNA content, alpha-tubulin and CP309 protein expression Fluorescence OpenBiosystems Drosophila RNAi Library 1.0 Mitotic regulator genes dsRNA Complex, sp Complex criteria
GR00128-A-2 39088 FBgn0005533 CG3922 DRSC32581 sp Synthetic lethal with methyl methanesulphonate no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00131-A-1 39088 RpS17 DRSC11271 25 - 50 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00152-A 39088 CG3922 RpS17 np 2.2297 Upregulation of Notch pathway after Notch stimulation no N(delta)ecn Notch stimulation; m3-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 39088 CG3922 RpS17 np -1.8117 none no without Notch stimulation Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00152-A 39088 CG3922 RpS17 np -0.16425 none no N(delta)ecn Notch stimulation; con-luc normalization Notch induced transcription Modifiers of notch transcriptional activity identified by genome-wide RNAi. Mourikis et al. 2010 20959007 Cell line Kc167 Notch pathway reporter Luminescence np Genome-wide dsRNA Z-score < -2 OR > 2 (con-luc) OR < -1.8 OR > 1.8 (m3-luc) Additional information about secondary screens
GR00170-A-1 39088 RpS17 DRSC11271 -5.8 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00175-A 39088 CG3922 RpS17 DRSC32582 -0.600366444246565 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00175-A 39088 CG3922 RpS17 DRSC32581 -0.203302446998994 none no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00214-A-1 39088 FBgn0005533 RpS17 DRSC11271 1.24 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 39088 FBgn0005533 RpS17 DRSC11271 -3.16 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 39088 FBgn0005533 RpS17 DRSC11271 -0.87 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 39088 FBgn0005533 RpS17 DRSC11271 2.75 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 39088 CG3922 RpS17 AMB27914 -1.99 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 39088 CG3922 3922R np Decreased Chp protein expression no not detectable Chp level Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00273-A-1 39088 FBgn0005533 RpS17 DRSC11271 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00291-A 39088 RpS17 DRSC11271 sp Increased or decreased hedgehog (hh) secretion eliminated as hit by authors, sp Hedgehog (hh) secretion regulation A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila Aikin et al. 2012 22432040 Cell line S2 Hedgehog protein expression Luminescence DRSC Genome-wide dsRNA Z-score < -2 OR > 2 by at least 1/3 Z-scores 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available.
GR00296-A 39088 RpS17 np 3.39 Increased Sindbis virus (SINV) infection prim. screen rep. B: 3.09; sec. val. screen: not tested Sindbis virus (SINV) infection Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry Panda et al. 2013 24332855 Cell line DL1 Sindbis virus (SINV) GFP protein expression Fluorescence Ambion Genome-wide dsRNA Z-score < -2 OR > 2 Primary screen performed in duplicate. Z-scores from replicate B and from secondary validation screen are shown in the comment field. Recombinant SINV (HRsp) expressing GFP was used for infection.
GR00308-A 39088 CG3922 RpS17 np 3 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 39088 FBgn0005533 RpS17 DRSC11271 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
39088 FBgn0005533 np np sp none