Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 41469
Symbol: ATP8B
Description: ATPase 8B

DataSource: http://genomernai.dkfz.de/v16/genedetails/41469

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	41469	FBgn0037989	CG14741	DRSC14961	-0.093083819	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	41469	FBgn0037989	CG14741	DRSC14961	0.695812794	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1	41469			HFA14961	0.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	41469			HFA14961	0.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00049-A-0	41469	FBgn0037989		DRSC14961	3	Decreased NFAT translocation		no		NFAT signaling	A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT.	Gwack et al.	2006	16511445	Cell line	S2R+	NFAT translocation	High content (microscopy)	DRSC		dsRNA	Visual inspection	np
GR00062-A	41469	FBgn0037989	CG14741	np	2	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00084-A-0	41469	FBgn0037989	CG14741	np	np	C. trachomatis infection down		no		C. trachomatis infection	RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry.	Elwell et al.	2008	18369471	Cell line	S2	C. trachomatis infection	High content (microscopy)	OpenBiosystems		dsRNA	Visual inspection	np
GR00148-A	41469	FBgn0037989	CG14741	np	2	Increased P-JNK protein expression with PGN	60 min PGN induction	no		PGN-induced dJNK phosphorylation	A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling.	Bond and Foley	2009	19893628	Cell line	S2	P-JNK protein expression	Fluorescence	Custom-made	Genome-wide	dsRNA	Z-score	Complex criteria	Only hits stored in GenomeRNAi
GR00214-A-1	41469	FBgn0037989	CG14741	DRSC14961	0.59	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	41469	FBgn0037989	CG14741	DRSC14961	0.25	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	41469	FBgn0037989	CG14741	DRSC14961	0.41	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	41469	FBgn0037989	CG14741	DRSC14961	1.8	Increased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	41469	CG14741		AMB25286	-0.57	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00284-A	41469	CG14741	CG14741	102648	0.06572531850727133	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	41469	FBgn0037989	CG14741	DRSC14961	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
	41469	FBgn0037989	np	np	sp	none
GR00367-S	41469	CG14741		102648	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	41469	CG14741		102648	-0.1710599851853496	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A	41469			102648	0.38	none				Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	41469	CG14741		KK102648		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.