| GR00002-A |
42300 |
FBgn0010768 |
sqz |
DRSC16980 |
-0.369892072 |
none |
|
yes |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
42300 |
FBgn0010768 |
sqz |
DRSC16980 |
-0.352675787 |
none |
|
yes |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00007-A-0 |
42300 |
FBgn0010768 |
|
DRSC16980 |
np |
Defasciculation |
|
no |
|
Neural outgrowth |
Identification of neural outgrowth genes using genome-wide RNAi. |
Sepp et al. |
2008 |
18604272 |
Cell line |
Primary neural cells |
Morphology |
High content (microscopy) |
DRSC |
|
dsRNA |
Visual inspection |
np |
|
GR00031-A-1 |
42300 |
|
|
HFA16980 |
-0.1 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
42300 |
|
|
HFA16980 |
-0.3 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
42300 |
FBgn0010768 |
sqz |
RE47124 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
42300 |
FBgn0010768 |
sqz |
RE64548 |
sp |
none |
|
no |
library: DGC2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00062-A |
42300 |
FBgn0010768 |
sqz |
np |
4 |
Decreased Ca2+ influx |
|
|
|
Ca2+ channel regulation |
CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. |
Vig et al. |
2006 |
16645049 |
Cell line |
S2R+ |
Ca2+ influx |
Fluorescence |
np |
Genome-wide |
dsRNA |
Complex, sp |
> |
|
GR00065-A |
42300 |
CG5557 |
sqz |
sqz |
np |
Decreased cell number |
|
yes |
|
Dendrite pattern formation |
Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. |
Parrish et al. |
2006 |
16547170 |
Tissue |
GAL4221 |
mCD8 protein expression |
Fluorescence |
Custom-made |
Transcription factors |
UAS-IR construct |
np |
Phenotypes in multiple blind tests |
Additional information about secondary screens |
GR00218-S |
42300 |
CG5557 |
sqz |
AMB30287 |
1.05 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
42300 |
CG5557 |
|
5557R |
sp |
Increased alpha1,3-fucose Chp glycosylation, increased GlcNAcb1-4GlcNAc Chp glycosylation, increased mannose Chp glycosylation |
|
yes |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00239-A-2 |
42300 |
CG5557 |
|
5557R |
np |
Effect on Chp glycosylation |
|
yes |
rank 1 |
Glycosylation regulation (2) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation |
Luminescence |
np |
Selected genes |
UAS-IR construct |
p-value |
< 0.005 |
|
GR00239-A-3 |
42300 |
CG5557 |
|
5557G |
np |
Effect on Chp glycosylation |
|
no |
library: custom-made |
Glycosylation regulation (3) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation |
Luminescence |
np |
Selected genes |
UAS-IR construct |
p-value |
< 0.05 |
|
GR00311-A |
42300 |
FBgn0010768 |
sqz |
DRSC16980 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
|
42300 |
FBgn0010768 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|