Gene2Function

RNAi

Gene Info

Species: Fly (Drosophila melanogaster)
GeneID: 42692
Symbol: Takl2
Description: Tak1-like 2

DataSource: http://genomernai.dkfz.de/v16/genedetails/42692

Export (tab separated) Export to Excel

Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	42692	FBgn0039015	Takl2	DRSC15650	-1.935525437	none		yes		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	42692	FBgn0039015	Takl2	DRSC15650	0.371302832	none		yes		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00030-A-1	42692	CG4803	Takl2	Takl2	np	none		yes		Cell cycle regulation (1)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content	Flow cytometry	rp	Kinases	dsRNA	Confidence intervals	Complex criteria
GR00030-A-2	42692	CG4803	Takl2	Takl2	np	none		no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	42692			HFA15650	0.4	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	42692			HFA15650	0.1	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	42692	FBgn0039015	Takl2	CT15429	sp	none		no	library: PHOSPHO
GR00134-A-1	42692	CG4803	Takl2	34898	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00139-A	42692		Takl2	34898	sp	none		no	viable	Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1	42692	CG4803	Takl2	34898	-0.36734693877551	none		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	42692	CG4803		34898	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00190-A-1	42692	FBgn0039015	Takl2	34898	-0.233	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00201-A-1	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		yes		Actin organization and cell morphology (1)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-2	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		yes		Actin organization and cell morphology (2)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2R+	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-3	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		yes		Actin organization and cell morphology (3)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	Kc167	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-4	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		yes		Actin organization and cell morphology (4)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG2-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-5	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		yes		Actin organization and cell morphology (5)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c1	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-6	42692	FBgn0039015	Takl2	CG4803 (UCSF), Takl2 (1) (Baum_Lab), Takl2 (2) (Baum_Lab)	np	none		no		Actin organization and cell morphology (6)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00202-A	42692	FBgn0039015	Takl2	np	sp	none		no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00214-A-1	42692	FBgn0039015	Takl2	DRSC15650	-0.07	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	42692	FBgn0039015	Takl2	DRSC15650	-0.16	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42692	FBgn0039015	Takl2	DRSC15650	0.46	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	42692	FBgn0039015	Takl2	DRSC15650	-1.56	Decreased ERK phosphorylation after sSpitz and EGF stimulation	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	42692	CG4803		AMB21860	0.74	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00282-A	42692	CG4803	Takl2	104701	np	none			not expressed	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	42692	CG4803	Takl2	34898	np	none			not expressed	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00311-A	42692	FBgn0039015	Takl2	DRSC15650	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	42692	CG4803	Takl2	104701	np	none				Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00326-A	42692	FBgn0039015	Takl2	104701	np	none				Synapse formation and maintenance	A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance	Valakh et al.	2012	22542760	Tissue	Elav-Gal4	Neuromuscular junction morphology	Fluorescence	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	For further phenotype explanations sp.
	42692	FBgn0039015	np	np	sp	none
GR00367-S	42692	CG4803		104701	np	none			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00370-A	42692	FBgn0039015	Takl2		-0.19	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	42692	FBgn0039015	Takl2		-0.49	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	42692	FBgn0039015	Takl2		-0.5	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00385-A	42692			104701	0.40090471283612705	none				Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	42692	CG4803		GD34898		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.