| GR00002-A |
44030 |
FBgn0010909 |
msn |
DRSC07990 |
0.647920652 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
44030 |
FBgn0010909 |
msn |
DRSC07990 |
1.15274303 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
44030 |
FBgn0010909 |
msn |
DRSC08498 |
-0.395824829 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00002-A |
44030 |
FBgn0010909 |
msn |
DRSC08498 |
-0.927058305 |
none |
|
no |
|
Lipid storage |
COPI complex is a regulator of lipid homeostasis. |
Beller et al. |
2008 |
19067489 |
Cell line |
Kc167 |
Nuclear to lipid droplet cross-sectional area |
Fluorescence |
np |
Genome-wide |
dsRNA |
B-score |
> 2 OR < -1.7 |
Additional information about a secondary screen with non-overlaping dsRNAs |
GR00026-A-0 |
44030 |
FBgn0010909 |
misshapen |
np |
np |
Morphology |
|
no |
|
Cell morphology |
A functional genomic analysis of cell morphology using RNA interference. |
Kiger et al. |
2003 |
14527345 |
Cell line |
Kc167, S2R+ |
Morphology |
High content (microscopy) |
Custom-made |
|
dsRNA |
Visual inspection |
np |
|
GR00030-A-1 |
44030 |
CG16973 |
msn |
msn |
np |
none |
|
yes |
|
Cell cycle regulation (1) |
Genome-wide survey of protein kinases required for cell cycle progression. |
Bettencourt-Dias et al. |
2004 |
15616552 |
Cell line |
S2 |
alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content |
Flow cytometry |
rp |
Kinases |
dsRNA |
Confidence intervals |
Complex criteria |
|
GR00030-A-2 |
44030 |
CG16973 |
msn |
msn |
np |
none |
|
no |
|
Cell cycle regulation (2) |
Genome-wide survey of protein kinases required for cell cycle progression. |
Bettencourt-Dias et al. |
2004 |
15616552 |
Cell line |
S2 |
Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression |
Flow cytometry |
rp |
Kinases and kinase regulators |
dsRNA |
np |
np |
|
GR00031-A-1 |
44030 |
|
|
HFA08498 |
0 |
none |
|
yes |
|
Cell growth and viability (1) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
Kc167 |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
GR00031-A-2 |
44030 |
|
|
HFA08498 |
-0.1 |
none |
|
no |
|
Cell growth and viability (2) |
Genome-wide RNAi analysis of growth and viability in Drosophila cells. |
Boutros et al. |
2004 |
14764878 |
Cell line |
S2R+ |
Cell number and viability |
Luminescence |
Custom-made (HFA) |
Genome-wide |
dsRNA |
Z-score |
> |
|
|
44030 |
FBgn0010909 |
msn |
CT37669 |
sp |
none |
|
no |
library: PHOSPHO |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
44030 |
FBgn0010909 |
msn |
np |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
44030 |
FBgn0010909 |
msn |
LD16337 |
sp |
none |
|
no |
library: DGC1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00148-A |
44030 |
FBgn0010909 |
msn |
np |
-3.15 |
Decreased P-JNK protein expression with PGN |
15 min PGN induction |
yes |
|
PGN-induced dJNK phosphorylation |
A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. |
Bond and Foley |
2009 |
19893628 |
Cell line |
S2 |
P-JNK protein expression |
Fluorescence |
Custom-made |
Genome-wide |
dsRNA |
Z-score |
Complex criteria |
Only hits stored in GenomeRNAi |
GR00191-A-1 |
44030 |
CG16973 |
msn |
np |
np |
Increased melanotic mass formation |
|
yes |
|
Blood cell homeostasis regulation (1) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00191-A-2 |
44030 |
CG16973 |
msn |
np |
np |
none |
|
yes |
|
Blood cell homeostasis regulation (2) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
np |
np |
|
GR00191-A-3 |
44030 |
CG16973 |
msn |
np |
4 |
none |
|
yes |
|
Blood cell homeostasis regulation (3) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-4 |
44030 |
CG16973 |
msn |
np |
0 |
none |
|
yes |
|
Blood cell homeostasis regulation (4) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-5 |
44030 |
CG16973 |
msn |
np |
31.6 |
Increased melanotic mass formation |
|
yes |
|
Blood cell homeostasis regulation (5) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
cg-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-6 |
44030 |
CG16973 |
msn |
np |
0 |
none |
|
yes |
non-overlapping dsRNA |
Blood cell homeostasis regulation (6) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
srp-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-7 |
44030 |
CG16973 |
msn |
np |
0 |
none |
|
yes |
non-overlapping dsRNA |
Blood cell homeostasis regulation (7) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
hml∆-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00191-A-8 |
44030 |
CG16973 |
msn |
np |
0 |
none |
|
no |
non-overlapping dsRNA |
Blood cell homeostasis regulation (8) |
An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. |
Avet-Rochex et al. |
2010 |
20540764 |
Tissue |
cg-GAL4 |
Melanotic mass formation |
Microscopy |
np |
Selected genes |
UAS-IR construct |
Tumor index |
> |
|
GR00201-A-1 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
none |
|
yes |
|
Actin organization and cell morphology (1) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
S2 |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00201-A-2 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
Altered cell morphology |
|
yes |
|
Actin organization and cell morphology (2) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
S2R+ |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00201-A-3 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
Altered cell morphology |
|
yes |
|
Actin organization and cell morphology (3) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
Kc167 |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00201-A-4 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
Altered cell morphology |
|
yes |
|
Actin organization and cell morphology (4) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
BG2-c2 |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00201-A-5 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
Altered cell morphology |
|
yes |
|
Actin organization and cell morphology (5) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
BG3-c1 |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00201-A-6 |
44030 |
FBgn0010909 |
msn |
msn (1) (Baum_Lab), msn (2) (Baum_Lab) |
np |
Altered cell morphology |
|
no |
|
Actin organization and cell morphology (6) |
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. |
Liu et al. |
2009 |
19265526 |
Cell line |
BG3-c2 |
alpha-Tubulin and F-actin protein expression |
Fluorescence |
Custom-made |
Kinases |
dsRNA |
Visual inspection |
np |
|
GR00202-A |
44030 |
FBgn0010909 |
msn |
np |
sp |
none |
|
no |
|
Vaccinia virus infection |
A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. |
Moser et al. |
2010 |
20585561 |
Cell line |
DL1 |
Virally encoded beta-galactosidase protein expression |
Fluorescence |
Custom-made |
Kinases, phosphatases and selected genes |
dsRNA |
Z-score |
< -2 in both duplicates |
Author-reviewed data |
GR00218-S |
44030 |
CG16973 |
msn |
AMB28900 |
0.94 |
none |
|
no |
|
Hippo pathway regulation |
Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila |
Wehr et al. |
2013 |
23263283 |
Cell line |
S2R+ |
Hippo pathway reporter |
Luminescence |
np |
Genome-wide |
dsRNA |
Z-score |
> 3 OR < -3 |
Author-submitted data. Primary screen |
GR00239-A-1 |
44030 |
CG16973 |
|
16973R |
sp |
none |
|
no |
|
Glycosylation regulation (1) |
Identification of genes required for neural-specific glycosylation using functional genomics. |
Yamamoto-Hino et al. |
2010 |
21203496 |
Tissue |
GMR-GAL4 |
Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability |
Luminescence |
np |
Selected genes |
UAS-IR construct |
Z-score |
> 3 |
|
GR00273-A-1 |
44030 |
FBgn0010909 |
msn |
DRSC25188 |
1 |
Effect on Polycomb (Pc) foci |
|
|
|
Polycomb group (PcG) proteins regulation (1) |
Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen |
Gonzalez et al. |
2014 |
24703951 |
Cell line |
S2 |
Pc-GFP protein localization |
Fluorescence |
DRSC |
Genome-wide and transcription factors |
dsRNA |
Visual inspection |
1 |
Additional phenotypic clustering screen available. |
GR00282-A |
44030 |
CG16973 |
msn |
101517 |
np |
none |
|
|
|
piRNA pathway regulation |
The Genetic Makeup of the Drosophila piRNA Pathway |
Handler |
2013 |
23665231 |
Tissue |
tj-GAL4 |
Gypsy transposon expression |
Microscopy |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data |
GR00284-A |
44030 |
CG16973 |
msn |
101517 |
-0.26794580566434595 |
none |
|
|
|
piRNA pathway regulation |
A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway |
Czech et al. |
2013 |
23665227 |
Tissue |
nos-GAL4 |
Transposon expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Weak < -1.5; strong: < -2 |
KK and GD libraries used (see phenotype data) |
GR00311-A |
44030 |
FBgn0010909 |
msn |
DRSC08498 |
np |
none |
|
|
|
Actin and microtubule morphology |
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype |
Rohn et al. |
2011 |
21893601 |
Cell line |
S2R+ |
Alpha-tubulin and F-actin protein expression |
Fluorescence |
DRSC |
Genome-wide |
dsRNA |
Visual inspection |
np |
Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. |
GR00326-A |
44030 |
FBgn0010909 |
msn |
101517 |
np |
none |
|
|
|
Synapse formation and maintenance |
A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance |
Valakh et al. |
2012 |
22542760 |
Tissue |
Elav-Gal4 |
Neuromuscular junction morphology |
Fluorescence |
VDRC |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
For further phenotype explanations sp. |
GR00335-A |
44030 |
FBgn0010909 |
msn |
JF03219 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
GR00335-A |
44030 |
FBgn0010909 |
msn |
GL00098 |
np |
none |
|
|
|
Germline stem cell (GSC) regulation |
A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal |
Yan et al. |
2014 |
24576427 |
Tissue |
MTD-GAL4 or UAS-dcr2; nanos-GAL4 |
Spectrin and vasa protein expression |
Fluorescence |
TRiP |
Selected genes |
UAS-IR construct |
Visual inspection |
np |
More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. |
|
44030 |
FBgn0010909 |
np |
np |
sp |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00367-S |
44030 |
CG16973 |
|
101517 |
np |
liquid clearance defect |
|
|
VDRC |
Drosophila airway maturation |
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes |
Hosono et al. |
2015 |
26551273 |
Tissue |
btl-GAL4 |
Liquid clearance defect and viability |
Visual inspection |
VDRC, Trip, NIG |
Genome-wide |
UAS-IR construct |
Frequency |
>30% |
|
GR00370-A |
44030 |
FBgn0010909 |
msn |
|
-2.19 |
Decreased CncC (Nrf2) reporter expression |
|
|
|
Regulation of Nrf2 homolog CncC |
Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes |
Li et al. |
2016 |
26911346 |
Cell line |
S2 |
CncC reporter |
Luminescence |
HD2 |
Kinases |
dsRNA |
Z-Score |
<-1.65 OR >1.65 |
|
GR00370-A |
44030 |
FBgn0010909 |
msn |
|
-1.48 |
none |
|
|
|
Regulation of Nrf2 homolog CncC |
Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes |
Li et al. |
2016 |
26911346 |
Cell line |
S2 |
CncC reporter |
Luminescence |
HD2 |
Kinases |
dsRNA |
Z-Score |
<-1.65 OR >1.65 |
|
GR00370-A |
44030 |
FBgn0010909 |
msn |
|
-2.05 |
Decreased CncC (Nrf2) reporter expression |
|
|
|
Regulation of Nrf2 homolog CncC |
Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes |
Li et al. |
2016 |
26911346 |
Cell line |
S2 |
CncC reporter |
Luminescence |
HD2 |
Kinases |
dsRNA |
Z-Score |
<-1.65 OR >1.65 |
|
GR00379-A-1 |
44030 |
CG16973 |
|
101517 |
-0.18424049079321897 |
none |
|
|
No phenotype in egg laying/hatching or larvae in egg laying or larvae |
Stem cell maintenance |
Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation |
Sanchez et al. |
2016 |
26669894 |
Organism |
nanos-GAL4 |
nos and yTub37c mRNA expression |
qPCR |
VDRC |
Selected genes |
UAS-IR construct |
Z-score |
Low: 1 - 2.5; Medium: 2.5 - 4; High: > |
Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. |
GR00388-A |
44030 |
CG16973 |
|
KK101517 |
|
Lethal |
|
|
|
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |
GR00388-A |
44030 |
CG16973 |
|
BL28791 |
|
Lethal |
|
|
ISC Overproliferation |
Regulation of intestinal stem cells (ISC) |
Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila |
Zeng et al. |
2015 |
25704823 |
Tissue |
act5C-GAL4 |
Viability |
Visual inspection |
VDRC and BDSC |
Genome-wide |
UAS-IR construct |
np |
np |
Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |