RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 45821
  • Symbol: Abl
  • Description: Abl tyrosine kinase
DataSource: http://genomernai.dkfz.de/v16/genedetails/45821

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 45821 FBgn0000017 Abl DRSC23306 -0.220224221 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45821 DRSC21286 -1.383260525 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45821 DRSC21286 2.701671638 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45821 FBgn0000017 Abl DRSC09661 -0.329610388 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45821 FBgn0000017 Abl DRSC09661 0.327600817 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 45821 FBgn0000017 Abl DRSC23306 0.700463033 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00026-A-0 45821 FBgn0000017 Abl tyrosine kinase np np Morphology no Cell morphology A functional genomic analysis of cell morphology using RNA interference. Kiger et al. 2003 14527345 Cell line Kc167, S2R+ Morphology High content (microscopy) Custom-made dsRNA Visual inspection np
GR00030-A-1 45821 CG4032 Abl Abl np none yes Cell cycle regulation (1) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content Flow cytometry rp Kinases dsRNA Confidence intervals Complex criteria
GR00030-A-2 45821 CG4032 Abl Abl np none no Cell cycle regulation (2) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression Flow cytometry rp Kinases and kinase regulators dsRNA np np
GR00031-A-1 45821 HFA09661 -0.1 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-1 45821 HFA21286 -0.6 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 45821 HFA21286 -0.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 45821 HFA09661 0.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
45821 FBgn0000017 Abl LD03455 sp none no library: DGC1
45821 FBgn0000017 Abl CT13380 sp none no library: PHOSPHO
GR00084-A-0 45821 FBgn0000017 CG4032 np np C. trachomatis infection down no C. trachomatis infection RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry. Elwell et al. 2008 18369471 Cell line S2 C. trachomatis infection High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00085-A-0 45821 FBgn0000017 CG4032 np np P. aeruginosa invasion decreased no P. aeruginosa infection RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization. Pielage et al. 2008 18369477 Cell line S2 P. aeruginosa infection Invasion assay Custom-made library dsRNA Percent decreased invasion 33%
GR00135-A-1 45821 CG4032 Abl 2897 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 45821 CG4032 2897 1 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-2 45821 CG4032 2897 9.79 Decreased viability no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00142-A-1 45821 CG4032 Abl 2897 1.05479452054794 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 45821 CG4032 Abl 2897 -1.93877551020408 Decreased viability after Serratia marcescens infection no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 45821 CG4032 2897 0 none no partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00190-A-1 45821 FBgn0000017 Abl 2897 1.044 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00191-A-1 45821 CG4032 Abl np np none yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 45821 CG4032 Abl np np none yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 45821 CG4032 Abl np 0 none yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 45821 CG4032 Abl np 0 none yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 45821 CG4032 Abl np 0 none no Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00201-A-1 45821 FBgn0000017 Abl CG4032 (UCSF) np Altered cell morphology yes Actin organization and cell morphology (1) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-2 45821 FBgn0000017 Abl CG4032 (UCSF) np Altered cell morphology yes Actin organization and cell morphology (2) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2R+ alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-3 45821 FBgn0000017 Abl CG4032 (UCSF) np none yes Actin organization and cell morphology (3) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line Kc167 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-4 45821 FBgn0000017 Abl CG4032 (UCSF) np Altered cell morphology yes Actin organization and cell morphology (4) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG2-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-5 45821 FBgn0000017 Abl CG4032 (UCSF) np Altered cell morphology yes Actin organization and cell morphology (5) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c1 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-6 45821 FBgn0000017 Abl CG4032 (UCSF) np Altered cell morphology no Actin organization and cell morphology (6) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00218-S 45821 CG4032 Abl AMB18733 2.73 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00238-S-1 45821 CG4032 Abl 110186 sp none no library: VDRC Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00238-S-1 45821 CG4032 Abl 4032R-2 sp none no library: NIG Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1 45821 CG4032 4032R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 45821 CG4032 Abl 2897 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 45821 CG4032 Abl 110186 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 45821 CG4032 Abl 110186 0.16889460881614538 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 45821 np np DRSC21286 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00311-A 45821 FBgn0000017 Abl DRSC09661 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 45821 CG4032 Abl 110186 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00330-A 45821 Abl DRSC09661 np Decreased androgen receptor (AR) reporter expression Androgen receptor (AR) regulation A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells Imberg-Kazdan et al. 2013 23403032 Cell line S2R+ Androgen receptor (AR) reporter Luminescence Whole Genome Drosophila Library (DRSC 2.0) Genome-wide dsRNA Complex, sp Complex criteria Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’.
GR00330-A 45821 Abl DRSC23306 np Decreased androgen receptor (AR) reporter expression Androgen receptor (AR) regulation A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells Imberg-Kazdan et al. 2013 23403032 Cell line S2R+ Androgen receptor (AR) reporter Luminescence Whole Genome Drosophila Library (DRSC 2.0) Genome-wide dsRNA Complex, sp Complex criteria Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’.
GR00335-A 45821 FBgn0000017 Abl JF02960 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 45821 FBgn0000017 Abl GL00234 np Embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
45821 np np DRSC21286 0.19 none fold change: 0.89
45821 FBgn0000017 np np sp none
GR00370-A 45821 FBgn0000017 Abl -0.5 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 45821 FBgn0000017 Abl 0.02 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 45821 FBgn0000017 Abl -0.82 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00379-A-1 45821 CG4032 110186 -0.2853144530964956 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 45821 CG4032 GD2897 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 45821 CG4032 BL28325 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.