RNAi

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 344658
• Symbol: SAMD7
• Description: sterile alpha motif domain containing 7

DataSource: http://genomernai.dkfz.de/v16/genedetails/344658

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00053-A	344658	NM_182610	SAMD7	np	sp	none		no		Genome stability	A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability.	Paulsen et al.	2009	19647519	Cell line	HeLa	gamma-H2AX phosphorylation and DNA content	Fluorescence	siARRAY human genome siRNA library	Genome-wide	siRNA	p-value	Complex criteria	Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A	344658	NM_182610	SAMD7	np	0.936	none		no		Combinatorial effect with paclitaxel	Synthetic lethal screen identification of chemosensitizer loci in cancer cells.	Whitehurst et al.	2007	17429401	Cell line	NCI-H1155	Viability (synthetic lethal)	ATP level	# G-005000-01	Genome-wide	siRNA	Paclitaxel/control ratio	Complex criteria	Additional information about 87 high-confidence hits
GR00056-A	344658	NM_182610	SAMD7	np	1.076	none		no		Melanogenesis	Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells.	Ganesan et al.	2008	19057677	Cell line	MNT-1	Melanin protein expression and viability	Absorbance and luminescence	rp	Genome-wide	siRNA	Normalized absorbance ratio	> 2 standard deviations below mean	Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1	344658	NM_182610	SAMD7	M-009146-00	1.61	none		no		Wnt/beta-catenin pathway regulation (1)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Z-score	> 4	Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2	344658	NM_182610	SAMD7	M-009146-00	sp	none		no		Wnt/beta-catenin pathway regulation (2)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Complex, SP	Complex criteria	Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00149-A-1	344658	344658	SAMD7	126521	-52.8	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	126521	-10.61	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	126522	71.26	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	126522	60.76	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	126523	105.59	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	126523	90.54	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	290243	62.68	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00149-A-1	344658	344658	SAMD7	290243	56.45	none		no		Ciliogenesis and cilium length (1)	Functional genomic screen for modulators of ciliogenesis and cilium length.	Kim et al.	2010	20393563	Cell line	htRPE	Smoothed protein expression	Fluorescence	Human druggable genome siRNA library V3.1	Druggable genes	siRNA	Normalized percent inhibition	> 1.5 OR < -1.5 standard deviations from mean
GR00180-A-1	344658	344658	SAMD7	PL-50079	0.903	none		no		Hepatitis C virus replication (1)	A functional genomic screen identifies cellular cofactors of hepatitis C virus replication.	Tai et al.	2009	19286138	Cell line	Huh7/Rep-Feo	HCV replicon RNA copy number	Luminescence	siARRAY Human Genome siRNA Library	Genome-wide	siRNA	q-value	Complex criteria
GR00184-A-1	344658	NM_182610	SAMD7	M-009146-00	-2.51208454586725	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (1)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	SMARTpool siRNA library	Genome-wide	siRNA	Z-score	< -2
GR00184-A-2	344658	NM_182610	SAMD7	D-009146-02	0.498654230914565	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	344658	NM_182610	SAMD7	D-009146-04	0.507064825089151	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	344658	NM_182610	SAMD7	D-009146-01	0.39889060670943	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-2	344658	NM_182610	SAMD7	D-009146-03	0.399232321949392	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (2)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	344658	NM_182610	SAMD7	D-009146-01	0.570548702545617	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	344658	NM_182610	SAMD7	D-009146-04	0.636651664631255	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	344658	NM_182610	SAMD7	D-009146-03	0.408637956182973	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-3	344658	NM_182610	SAMD7	D-009146-02	0.462216625266652	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (3)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	344658	NM_182610	SAMD7	D-009146-02	0.345681546910509	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	344658	NM_182610	SAMD7	D-009146-01	0.428283120322507	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	344658	NM_182610	SAMD7	D-009146-03	0.202718745356136	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-4	344658	NM_182610	SAMD7	D-009146-04	0.476461318430024	Decreased POU5F1-GFP protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (4)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	344658	NM_182610	SAMD7	D-009146-02	0.749908371228245	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	344658	NM_182610	SAMD7	D-009146-04	0.92571007500296	none		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	344658	NM_182610	SAMD7	D-009146-03	0.760787227132195	none		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-5	344658	NM_182610	SAMD7	D-009146-01	0.671948672453201	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (5)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	344658	NM_182610	SAMD7	D-009146-01	0.783640501705393	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	344658	NM_182610	SAMD7	D-009146-04	0.908849168427166	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	344658	NM_182610	SAMD7	D-009146-02	0.822727860675576	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-6	344658	NM_182610	SAMD7	D-009146-03	0.84859369160593	none		yes		Self-renewal and pluripotency in human embryonic stem cells (6)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES2	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	344658	NM_182610	SAMD7	D-009146-03	0.714306942629338	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	344658	NM_182610	SAMD7	D-009146-02	0.57062405529048	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	344658	NM_182610	SAMD7	D-009146-04	0.902692112704363	none		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-7	344658	NM_182610	SAMD7	D-009146-01	0.654918530712863	Decreased OCT4 protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (7)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	OCT4 protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	344658	NM_182610	SAMD7	D-009146-01	0.640935575150617	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	344658	NM_182610	SAMD7	D-009146-03	0.739039744576765	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	344658	NM_182610	SAMD7	D-009146-04	0.777023251852725	none		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00184-A-8	344658	NM_182610	SAMD7	D-009146-02	0.537407757716909	Decreased NANOG protein expression		yes		Self-renewal and pluripotency in human embryonic stem cells (8)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	HES3	NANOG protein expression	Fluorescence	siGENOME siRNA library	Selected genes	siRNA	Relative expression	< 0.75
GR00197-A-1	344658	344658	SAMD7	M-009146-00	0.193428821	none		no		Human papillomavirus oncogene expression regulation (1)	Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.	Smith et al.	2010	20133580	Cell line	C33A/BE2/18LCR c4	HPV18 LCR reporter activity	Luminescence	Human siGENOME SMARTpool library	Genome-wide	siRNA	Z-score	>	Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00236-A-1	344658	344658	SAMD7	M-009146-00	0.747322631249811	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response.	Adamson et al.	2012	22344029	Cell line	DR-U2OS	(HR-DSBR) DR-GFP reporter and DNA content	Fluorescence	Human siGENOME siRNA (G-005000-05)	Genome-wide	siRNA	Relative HR ratio	< ~0.4 OR > 1.88	Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1	344658	NM_182610	SAMD7	M-009146-00	1.26	none		yes		TRAIL-induced apoptosis (1)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Z-score	> 4	Author-submitted data
GR00240-S-2	344658	NM_182610	SAMD7	M-009146-00	-1.25	none		no	Z-score 0.006	TRAIL-induced apoptosis (2)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability (synthetic lethal)	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Differential score	> 3.6 AND viability Z-score < 4	Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1	344658	NM_182610	SAMD7	np	sp	none		no		Selective autophagy regulation (1)	Image-based genome-wide siRNA screen identifies selective autophagy factors.	Orvedahl et al.	2011	22020285	Cell line	HeLa/GFP-LC3	Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression	Fluorescence	siGenome	Genome-wide	siRNA	Z-score	Complex criteria
GR00247-A-1	344658		SAMD7	np	sp	none			rank: 14567	Regulation of FOXO1 nuclear localization (1)	Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain.	Senapedis et al.	2011	21460183	Cell line	U2OS	EGFP-FOXO1a protein expression and DNA content	Fluorescence	Human Genome library	Genome-wide	siRNA	Complex, sp	Complex criteria
GR00249-S	344658	344658	SAMD7	J-009146-17	0.42757	none		no	number of cells compared to control (%): 105.28	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	344658	344658	SAMD7	M-009146-01	0.13123	none		no	number of cells compared to control (%): 94.54	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	344658	344658	SAMD7	s51077	0.01961	none		no	number of cells compared to control (%): 88.61	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	344658	344658	SAMD7	s51078	0.2465	none		no	number of cells compared to control (%): 91.53	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	344658	344658	SAMD7	s51079	0.87271	none		no	number of cells compared to control (%): 81.02	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00252-A-1	344658	344658	SAMD7	np	np	none				Oncolytic Maraba rhabdovirus infection (1)	Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death.	Mahoney et al.	2011	22014571	Cell line	OVCAR-8	Viability	Fluorescence	np	Genome-wide	siRNA	Composite MAD	np	All listed genes are final hits. Final hit if hit in >
GR00252-A-2	344658	344658	SAMD7	np	-2.51076	Decreased viability after Maraba virus infection				Oncolytic Maraba rhabdovirus infection (2)	Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death.	Mahoney et al.	2011	22014571	Cell line	U373	Viability	Fluorescence	np	Genome-wide	siRNA	Composite MAD	np	All listed genes are final hits. Final hit if hit in >
GR00252-A-3	344658	344658	SAMD7	np	-2.13509	Decreased viability after Maraba virus infection				Oncolytic Maraba rhabdovirus infection (3)	Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death.	Mahoney et al.	2011	22014571	Cell line	NCI-H226	Viability	Fluorescence	np	Genome-wide	siRNA	Composite MAD	np	All listed genes are final hits. Final hit if hit in >
GR00253-A	344658	NM_182610	SAMD7	np	-0.962	none				hepcidin regulation	Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling.	Mleczko-Sanecka et al.	2014	24385536	Cell line	Huh7	hepcidin::fluc mRNA expression	Luminescence	siGenome siARRAY SMARTpool	Genome-wide	siRNA	Z-score	>	Cutoff <
GR00255-A-1	344658	344658	SAMD7	TRCN0000134301, TRCN0000135097, TRCN0000136034, TRCN0000136212, TRCN0000136387	0.313033168	none				Negative genetic interactions (1)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2	344658	344658	SAMD7	TRCN0000134301, TRCN0000135097, TRCN0000136034, TRCN0000136212, TRCN0000136387	-0.178775179	none				Negative genetic interactions (2)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3	344658	344658	SAMD7	TRCN0000134301, TRCN0000135097, TRCN0000136034, TRCN0000136212, TRCN0000136387	0.248618288	none				Negative genetic interactions (3)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4	344658	344658	SAMD7	TRCN0000134301, TRCN0000135097, TRCN0000136034, TRCN0000136212, TRCN0000136387	-0.034282816	none				Negative genetic interactions (4)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5	344658	344658	SAMD7	TRCN0000134301, TRCN0000135097, TRCN0000136034, TRCN0000136212, TRCN0000136387	-0.514000805	none				Negative genetic interactions (5)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -0.8	HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00293-A	344658		SAMD7	np	-2.678	Decreased viability with paclitaxel				Combinatorial effect with paclitaxel	Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death	Sinnott et al.	2014	24860162	Cell line	HCC366	Viability	Luminescence	Thermo-Fisher	Genome-wide	siRNA	Z-score	< -2.5	Final hits according to the author are indicated in the comment.
GR00300-A	344658		SAMD7	TRCN0000136387, TRCN0000135798, TRCN0000135097, TRCN0000134330, TRCN0000136212, TRCN0000138452, TRCN0000136034, TRCN0000134905, TRCN0000134301, TRCN0000133810	0	none				Combinatorial effect with RAF inhibitor PLX4720	A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition.	Whittaker et al.	2013	23288408	Cell line	A375	shRNA abundance	Sequencing	TRC	Genome-wide	shRNA	Number of shRNAs ranked Top1000	> 2	The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A	344658	NM_182610	SAMD7	np	-0.48	none				Clear cell renal cell carcinoma (ccRCC) survival regulation	Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target	Gerlinger et al.	2012	22362593	Cell line	VHL-deficient RCC4	Proliferation and Viability	Fluorescence	np	Genome-wide	siRNA	Z-score	<	In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1	344658	344658	SAMD7	np	0.03	none				Sindbis virus (SINV) infection (1)	Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry	Ooi et al.	2013	24367265	Cell line	U2OS	Sindbis virus (SINV) reporter	Luminescence	Ambion Silencer V3	Genome-wide	siRNA	Z-score	< -3 OR > 2
	344658	344658	SAMD7	np	47.1	none			61% viability
GR00313-A	344658	NM_182610	SAMD7	np	-0.81	none				TNF-alpha pathway regulation	A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling.	Nickles et al.	2012	22733992	Cell line	HEK293T	NFkappaB pathway reporter	Luminescence	Qiagen	Genome-wide	siRNA	Z-score	<	Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
GR00363-A	344658	344658	SAMD7	SI00710402, SI00710395, 126522, SI00710416, SI00710409, 126521	sp	Increased transferrin (TF) endocytosis			strong	Endocytosis regulation	Systems survey of endocytosis by multiparametric image analysis.	Collinet et al.	2010	20190736	Cell line	HeLa	Transferrin (TF) and epidermal growth factor (EGF) subcellular localisation, DNA content	Fluorescence	Ambion, Qiagen and esiRNA library (Kittler et al.)	Genome-wide	siRNA	Complex, sp	Complex criteria	Two hit categories: "strong" (maximum of Prob Chi2, Prob Chi2 EGF, Prob Chi2 Tfn > 0.95) and "weak but specific" (maximum of Prob Chi2, Prob Chi2 EGF, Prob Chi2 Tfn < 0.95 but Phenoscore > 0.95). Additional information about secondary screens with kinase and phospatase libraries.
	344658	344658	SAMD7		-0.010000000000000009	none
GR00376-A-1	344658	344658	SAMD7		0.001188399	none				Mitigators of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2	344658	344658	SAMD7		0.531144992	none				Sensitizers of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
GR00378-A	344658		SAMD7		-1.097049313	none				Poliovirus vaccine production	Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game	van der Sanden et al.	2015	26581994	Cell line	HEp-2C	Infection with Attenuated Poliovirus	ELISA	Dharmacon	Genome-wide	siRNA	Z-score	>	For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol
	344658	NM_182610	SAMD7		51.652	none
GR00386-A-1	344658	344658	SAMD7		63.8991171984531	Decreased viability				NOD2 stimulation by MDP	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	Viability	Luminescence	Dharmacon	Genome-wide	siRNA	Percentage growth	Decreased: <70, increased: >120	Reagent IDs not provided
GR00386-A-2	344658	344658	SAMD7		-244.6	none				MDP-induced IL-8 secretion	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	IL-8 secretion	ELISA	Dharmacon	Genome-wide	siRNA	Percent inhibition of IL-8 secretion	Increased: <-300, Decreased: >60	Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided