| GR00053-A |
4648 |
XM_291001 |
MYO7B |
np |
sp |
Increased gamma-H2AX phosphorylation |
|
no |
group 3 |
Genome stability |
A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. |
Paulsen et al. |
2009 |
19647519 |
Cell line |
HeLa |
gamma-H2AX phosphorylation and DNA content |
Fluorescence |
siARRAY human genome siRNA library |
Genome-wide |
siRNA |
p-value |
Complex criteria |
Confidence groupings from 4 to 1 (highest level of confidence in group 4) |
GR00054-A |
4648 |
XM_291001 |
MYO7B |
np |
0.848 |
none |
|
no |
|
Combinatorial effect with paclitaxel |
Synthetic lethal screen identification of chemosensitizer loci in cancer cells. |
Whitehurst et al. |
2007 |
17429401 |
Cell line |
NCI-H1155 |
Viability (synthetic lethal) |
ATP level |
# G-005000-01 |
Genome-wide |
siRNA |
Paclitaxel/control ratio |
Complex criteria |
Additional information about 87 high-confidence hits |
GR00056-A |
4648 |
XM_291001 |
MYO7B |
np |
0.89 |
none |
|
no |
|
Melanogenesis |
Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. |
Ganesan et al. |
2008 |
19057677 |
Cell line |
MNT-1 |
Melanin protein expression and viability |
Absorbance and luminescence |
rp |
Genome-wide |
siRNA |
Normalized absorbance ratio |
> 2 standard deviations below mean |
Additional information about a secondary screen (retest to determine false-positive rate) |
GR00057-A-1 |
4648 |
XM_371555 |
LOC389031 |
M-027951-00 |
0.25 |
none |
|
no |
|
Wnt/beta-catenin pathway regulation (1) |
A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. |
Tang et al. |
2008 |
18621708 |
Cell line |
HeLa |
Wnt pathway reporter |
Luminescence |
Human siArray siRNA library |
Genome-wide |
siRNA |
Z-score |
> 4 |
Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). |
GR00057-A-1 |
4648 |
XM_291001 |
MYO7B |
M-022928-00 |
-0.73 |
none |
|
no |
|
Wnt/beta-catenin pathway regulation (1) |
A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. |
Tang et al. |
2008 |
18621708 |
Cell line |
HeLa |
Wnt pathway reporter |
Luminescence |
Human siArray siRNA library |
Genome-wide |
siRNA |
Z-score |
> 4 |
Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). |
GR00057-A-2 |
4648 |
XM_371555 |
LOC389031 |
M-027951-00 |
sp |
none |
|
no |
|
Wnt/beta-catenin pathway regulation (2) |
A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. |
Tang et al. |
2008 |
18621708 |
Cell line |
HeLa |
Wnt pathway reporter |
Luminescence |
Human siArray siRNA library |
Genome-wide |
siRNA |
Complex, SP |
Complex criteria |
Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). |
GR00057-A-2 |
4648 |
XM_291001 |
MYO7B |
M-022928-00 |
sp |
none |
|
no |
|
Wnt/beta-catenin pathway regulation (2) |
A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. |
Tang et al. |
2008 |
18621708 |
Cell line |
HeLa |
Wnt pathway reporter |
Luminescence |
Human siArray siRNA library |
Genome-wide |
siRNA |
Complex, SP |
Complex criteria |
Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). |
GR00098-A-1 |
4648 |
ENSG00000169994 |
MYO7B |
ENSG00000169994 |
sp |
none |
|
no |
|
Cell division (1) |
Genome-scale RNAi profiling of cell division in human tissue culture cells. |
Kittler et al. |
2007 |
17994010 |
Cell line |
HeLa |
Cell number and DNA content |
Laser scanning cytometry |
rp |
Genome-wide |
esiRNA |
Complex, sp |
Complex criteria |
|
GR00151-A-1 |
4648 |
ENSG00000169994 |
MYO7B |
np |
-1.238 |
none |
|
no |
|
Homologous recombination DNA double-strand break repair (HR-DSBR) (1) |
A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. |
SÅ‚abicki et al. |
2010 |
20613862 |
Cell line |
HeLa |
(HR-DSBR) DR-GFP reporter |
Flow cytometry |
Custom-made |
Genome-wide |
esiRNA |
Z-score |
< -2 OR > 2 |
|
GR00180-A-1 |
4648 |
4648 |
MYO7B |
PL-50049 |
0.928 |
none |
|
no |
|
Hepatitis C virus replication (1) |
A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. |
Tai et al. |
2009 |
19286138 |
Cell line |
Huh7/Rep-Feo |
HCV replicon RNA copy number |
Luminescence |
siARRAY Human Genome siRNA Library |
Genome-wide |
siRNA |
q-value |
Complex criteria |
|
GR00184-A-1 |
4648 |
XM_291001 |
MYO7B |
M-022928-00 |
-1.03740740332738 |
none |
|
no |
|
Self-renewal and pluripotency in human embryonic stem cells (1) |
A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. |
Chia et al. |
2010 |
20953172 |
Cell line |
hESC H1 |
POU5F1 protein expression |
Fluorescence |
SMARTpool siRNA library |
Genome-wide |
siRNA |
Z-score |
< -2 |
|
GR00184-A-1 |
4648 |
XM_371555 |
LOC389031 |
M-027951-00 |
-0.503438746547441 |
none |
|
no |
|
Self-renewal and pluripotency in human embryonic stem cells (1) |
A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. |
Chia et al. |
2010 |
20953172 |
Cell line |
hESC H1 |
POU5F1 protein expression |
Fluorescence |
SMARTpool siRNA library |
Genome-wide |
siRNA |
Z-score |
< -2 |
|
GR00196-A-1 |
4648 |
ENSG00000169994 |
|
np |
sp |
none |
|
no |
|
TP53 interactions (1) |
A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. |
Krastev et al. |
2011 |
21642980 |
Cell line |
HCT116 ( wildtype and TP53 knockout) |
TP53 protein expression and viability |
Fluorescence |
rp |
Genome-wide |
esiRNA |
Complex, sp |
Complex criteria |
|
GR00197-A-1 |
4648 |
389031 |
LOC389031 |
M-027951-00 |
0.352709609 |
none |
|
no |
|
Human papillomavirus oncogene expression regulation (1) |
Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. |
Smith et al. |
2010 |
20133580 |
Cell line |
C33A/BE2/18LCR c4 |
HPV18 LCR reporter activity |
Luminescence |
Human siGENOME SMARTpool library |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5 |
GR00197-A-1 |
4648 |
4648 |
MYO7B |
M-022928-00 |
1.598643703 |
none |
|
no |
|
Human papillomavirus oncogene expression regulation (1) |
Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. |
Smith et al. |
2010 |
20133580 |
Cell line |
C33A/BE2/18LCR c4 |
HPV18 LCR reporter activity |
Luminescence |
Human siGENOME SMARTpool library |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5 |
GR00233-A-1 |
4648 |
4648 |
MYO7B |
np |
np |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection |
|
yes |
siRNA pool validated |
Hepatitis C virus (HCV) infection (1) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after virus infection |
Fluorescence |
Human Genome siARRAY siRNA G-005000–05 |
Genome-wide |
siRNA |
Percentage of infected cells |
< |
|
GR00233-A-2 |
4648 |
4648 |
MYO7B |
D-022928-03 |
0.38 |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection |
|
no |
|
Hepatitis C virus (HCV) infection (2) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after direct virus infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-2 |
4648 |
4648 |
MYO7B |
D-022928-02 |
0.11 |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection |
|
no |
|
Hepatitis C virus (HCV) infection (2) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after direct virus infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-2 |
4648 |
4648 |
MYO7B |
D-022928-04 |
1 |
none |
|
no |
|
Hepatitis C virus (HCV) infection (2) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after direct virus infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-2 |
4648 |
4648 |
MYO7B |
D-022928-01 |
0.98 |
none |
|
no |
|
Hepatitis C virus (HCV) infection (2) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after direct virus infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-3 |
4648 |
4648 |
MYO7B |
D-022928-03 |
0.34 |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection |
|
no |
|
Hepatitis C virus (HCV) infection (3) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after viral supernatant infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-3 |
4648 |
4648 |
MYO7B |
D-022928-02 |
0.16 |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection |
|
no |
|
Hepatitis C virus (HCV) infection (3) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after viral supernatant infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-3 |
4648 |
4648 |
MYO7B |
D-022928-04 |
0.56 |
none |
|
no |
|
Hepatitis C virus (HCV) infection (3) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after viral supernatant infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00233-A-3 |
4648 |
4648 |
MYO7B |
D-022928-01 |
0.48 |
Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection |
|
no |
|
Hepatitis C virus (HCV) infection (3) |
A genome-wide genetic screen for host factors required for hepatitis C virus propagation. |
Li et al. |
2009 |
19717417 |
Cell line |
Huh7.5.1 |
HCV core protein expression and DNA content after viral supernatant infection |
Fluorescence |
siARRAY siRNA |
Selected genes |
siRNA |
Normalized percentage of infected cells |
< |
|
GR00236-A-1 |
4648 |
389031 |
LOC389031 |
M-027951-00 |
1.00479931260731 |
none |
|
no |
|
Homologous recombination DNA double-strand break repair (HR-DSBR) (1) |
A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. |
Adamson et al. |
2012 |
22344029 |
Cell line |
DR-U2OS |
(HR-DSBR) DR-GFP reporter and DNA content |
Fluorescence |
Human siGENOME siRNA (G-005000-05) |
Genome-wide |
siRNA |
Relative HR ratio |
< ~0.4 OR > 1.88 |
Cutoff values correspond 2 standard deviations from the screen-wide mean |
GR00236-A-1 |
4648 |
4648 |
MYO7B |
M-022928-00 |
0.766181093734394 |
none |
|
no |
|
Homologous recombination DNA double-strand break repair (HR-DSBR) (1) |
A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. |
Adamson et al. |
2012 |
22344029 |
Cell line |
DR-U2OS |
(HR-DSBR) DR-GFP reporter and DNA content |
Fluorescence |
Human siGENOME siRNA (G-005000-05) |
Genome-wide |
siRNA |
Relative HR ratio |
< ~0.4 OR > 1.88 |
Cutoff values correspond 2 standard deviations from the screen-wide mean |
GR00240-S-1 |
4648 |
XM_291001 |
MYO7B |
M-022928-00 |
-0.18 |
none |
|
yes |
|
TRAIL-induced apoptosis (1) |
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. |
Kranz and Boutros |
2014 |
24442637 |
Cell line |
U251MG |
Viability |
Luminescence |
SMART-pool siRNA |
Genome-wide |
siRNA |
Z-score |
> 4 |
Author-submitted data |
GR00240-S-1 |
4648 |
XM_371555 |
LOC389031 |
M-027951-00 |
0.51 |
none |
|
yes |
|
TRAIL-induced apoptosis (1) |
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. |
Kranz and Boutros |
2014 |
24442637 |
Cell line |
U251MG |
Viability |
Luminescence |
SMART-pool siRNA |
Genome-wide |
siRNA |
Z-score |
> 4 |
Author-submitted data |
GR00240-S-2 |
4648 |
XM_291001 |
MYO7B |
M-022928-00 |
-0.03 |
none |
|
no |
Z-score -0.2285 |
TRAIL-induced apoptosis (2) |
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. |
Kranz and Boutros |
2014 |
24442637 |
Cell line |
U251MG |
Viability (synthetic lethal) |
Luminescence |
SMART-pool siRNA |
Genome-wide |
siRNA |
Differential score |
> 3.6 AND viability Z-score < 4 |
Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen. |
GR00240-S-2 |
4648 |
XM_371555 |
LOC389031 |
M-027951-00 |
-1.35 |
none |
|
no |
Z-score -0.7605 |
TRAIL-induced apoptosis (2) |
A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. |
Kranz and Boutros |
2014 |
24442637 |
Cell line |
U251MG |
Viability (synthetic lethal) |
Luminescence |
SMART-pool siRNA |
Genome-wide |
siRNA |
Differential score |
> 3.6 AND viability Z-score < 4 |
Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen. |
GR00242-A-1 |
4648 |
XM_291001 |
MYO7B |
np |
sp |
none |
|
no |
|
Selective autophagy regulation (1) |
Image-based genome-wide siRNA screen identifies selective autophagy factors. |
Orvedahl et al. |
2011 |
22020285 |
Cell line |
HeLa/GFP-LC3 |
Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression |
Fluorescence |
siGenome |
Genome-wide |
siRNA |
Z-score |
Complex criteria |
|
GR00247-A-1 |
4648 |
|
MYO7B |
np |
sp |
none |
|
|
rank: 1614 |
Regulation of FOXO1 nuclear localization (1) |
Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. |
Senapedis et al. |
2011 |
21460183 |
Cell line |
U2OS |
EGFP-FOXO1a protein expression and DNA content |
Fluorescence |
Human Genome library |
Genome-wide |
siRNA |
Complex, sp |
Complex criteria |
|
GR00249-S |
4648 |
4648 |
MYO7B |
J-022928-05 |
-0.52619 |
none |
|
no |
number of cells compared to control (%): 89.10 |
Vaccinia virus (VACV) infection |
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. |
Sivan et al. |
2013 |
23401514 |
Cell line |
HeLa |
Vaccinia virus VACV IHD-J/GFP protein expression and DNA content |
Fluorescence |
Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. |
GR00249-S |
4648 |
4648 |
MYO7B |
M-022928-01 |
0.05319 |
none |
|
no |
number of cells compared to control (%): 93.03 |
Vaccinia virus (VACV) infection |
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. |
Sivan et al. |
2013 |
23401514 |
Cell line |
HeLa |
Vaccinia virus VACV IHD-J/GFP protein expression and DNA content |
Fluorescence |
Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. |
GR00249-S |
4648 |
4648 |
MYO7B |
s9217 |
0.00078 |
none |
|
no |
number of cells compared to control (%): 81.25 |
Vaccinia virus (VACV) infection |
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. |
Sivan et al. |
2013 |
23401514 |
Cell line |
HeLa |
Vaccinia virus VACV IHD-J/GFP protein expression and DNA content |
Fluorescence |
Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. |
GR00249-S |
4648 |
4648 |
MYO7B |
s9218 |
0.36229 |
none |
|
no |
number of cells compared to control (%): 84.72 |
Vaccinia virus (VACV) infection |
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. |
Sivan et al. |
2013 |
23401514 |
Cell line |
HeLa |
Vaccinia virus VACV IHD-J/GFP protein expression and DNA content |
Fluorescence |
Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. |
GR00249-S |
4648 |
4648 |
MYO7B |
s9219 |
0.61465 |
none |
|
no |
number of cells compared to control (%): 84.80 |
Vaccinia virus (VACV) infection |
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. |
Sivan et al. |
2013 |
23401514 |
Cell line |
HeLa |
Vaccinia virus VACV IHD-J/GFP protein expression and DNA content |
Fluorescence |
Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus |
Genome-wide |
siRNA |
Z-score |
> |
Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. |
GR00253-A |
4648 |
XM_291001 |
MYO7B |
np |
-0.592 |
none |
|
|
|
hepcidin regulation |
Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling. |
Mleczko-Sanecka et al. |
2014 |
24385536 |
Cell line |
Huh7 |
hepcidin::fluc mRNA expression |
Luminescence |
siGenome siARRAY SMARTpool |
Genome-wide |
siRNA |
Z-score |
> |
Cutoff < |
GR00293-A |
4648 |
|
MYO7B |
np |
0.443 |
none |
|
|
|
Combinatorial effect with paclitaxel |
Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death |
Sinnott et al. |
2014 |
24860162 |
Cell line |
HCC366 |
Viability |
Luminescence |
Thermo-Fisher |
Genome-wide |
siRNA |
Z-score |
< -2.5 |
Final hits according to the author are indicated in the comment. |
GR00303-A |
4648 |
XM_291001 |
MYO7B |
np |
1.31 |
none |
|
|
|
Clear cell renal cell carcinoma (ccRCC) survival regulation |
Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target |
Gerlinger et al. |
2012 |
22362593 |
Cell line |
VHL-deficient RCC4 |
Proliferation and Viability |
Fluorescence |
np |
Genome-wide |
siRNA |
Z-score |
< |
In the phenotype data duplicates were in the original document, which have been removed. |
GR00310-A-1 |
4648 |
|
MYO7B |
np |
0.32 |
none |
|
|
|
Sindbis virus (SINV) infection (1) |
Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry |
Ooi et al. |
2013 |
24367265 |
Cell line |
U2OS |
Sindbis virus (SINV) reporter |
Luminescence |
Ambion Silencer V3 |
Genome-wide |
siRNA |
Z-score |
< -3 OR > 2 |
|
|
4648 |
4648 |
MYO7B |
np |
-19.8 |
none |
|
|
118,4% viability |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00327-A |
4648 |
4648 |
SMAD2 |
CLL-H-023970 |
-0.9786156561208788 |
none |
|
|
|
Tumor formation |
An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation |
Wolf et al. |
2013 |
24292671 |
Cell line |
SUM-149 |
shRNA abundance |
Next-generation sequencing |
Decipher library module 1 |
Selected genes |
shRNA |
Z-score |
> 2.24 |
Genes were scored as hit if at least 2 shRNAs scored as hit, see comment. |
GR00327-A |
4648 |
4648 |
SMAD2 |
CLL-H-023971 |
-0.8882498978630353 |
none |
|
|
|
Tumor formation |
An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation |
Wolf et al. |
2013 |
24292671 |
Cell line |
SUM-149 |
shRNA abundance |
Next-generation sequencing |
Decipher library module 1 |
Selected genes |
shRNA |
Z-score |
> 2.24 |
Genes were scored as hit if at least 2 shRNAs scored as hit, see comment. |
GR00327-A |
4648 |
4648 |
SMAD2 |
CLL-H-023967 |
0.14805548894031167 |
none |
|
|
|
Tumor formation |
An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation |
Wolf et al. |
2013 |
24292671 |
Cell line |
SUM-149 |
shRNA abundance |
Next-generation sequencing |
Decipher library module 1 |
Selected genes |
shRNA |
Z-score |
> 2.24 |
Genes were scored as hit if at least 2 shRNAs scored as hit, see comment. |
GR00327-A |
4648 |
4648 |
SMAD2 |
CLL-H-023969 |
-0.39532464214238133 |
none |
|
|
|
Tumor formation |
An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation |
Wolf et al. |
2013 |
24292671 |
Cell line |
SUM-149 |
shRNA abundance |
Next-generation sequencing |
Decipher library module 1 |
Selected genes |
shRNA |
Z-score |
> 2.24 |
Genes were scored as hit if at least 2 shRNAs scored as hit, see comment. |
GR00327-A |
4648 |
4648 |
SMAD2 |
CLL-H-023968 |
0.7611320223589426 |
none |
|
|
|
Tumor formation |
An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation |
Wolf et al. |
2013 |
24292671 |
Cell line |
SUM-149 |
shRNA abundance |
Next-generation sequencing |
Decipher library module 1 |
Selected genes |
shRNA |
Z-score |
> 2.24 |
Genes were scored as hit if at least 2 shRNAs scored as hit, see comment. |
GR00363-A |
4648 |
4648 |
MYO7B |
SI00653639, SI00653639_3, SI00653639_2, SI00653639_1, esi12338 |
sp |
Decreased epidermal growth factor (EGF) endocytosis, increased transferrin (TF) endocytosis |
|
|
strong |
Endocytosis regulation |
Systems survey of endocytosis by multiparametric image analysis. |
Collinet et al. |
2010 |
20190736 |
Cell line |
HeLa |
Transferrin (TF) and epidermal growth factor (EGF) subcellular localisation, DNA content |
Fluorescence |
Ambion, Qiagen and esiRNA library (Kittler et al.) |
Genome-wide |
siRNA |
Complex, sp |
Complex criteria |
Two hit categories: "strong" (maximum of Prob Chi2, Prob Chi2 EGF, Prob Chi2 Tfn > 0.95) and "weak but specific" (maximum of Prob Chi2, Prob Chi2 EGF, Prob Chi2 Tfn < 0.95 but Phenoscore > 0.95). Additional information about secondary screens with kinase and phospatase libraries. |
GR00376-A-1 |
4648 |
4648 |
MYO7B |
|
0.708679722 |
none |
|
|
|
Mitigators of SS1P-induced immunotoxicity |
Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity |
Pasetto et al. |
2015 |
25713356 |
Cell line |
KB cells |
Viability |
Luminescence |
Ambion Silencer Select Version 4 |
Genome-wide |
siRNA |
RSA P-value |
<0.001 |
SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID |
GR00376-A-2 |
4648 |
4648 |
MYO7B |
|
0.525621642 |
none |
|
|
|
Sensitizers of SS1P-induced immunotoxicity |
Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity |
Pasetto et al. |
2015 |
25713356 |
Cell line |
KB cells |
Viability |
Luminescence |
Ambion Silencer Select Version 4 |
Genome-wide |
siRNA |
RSA P-value |
<0.001 |
SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID |
GR00378-A |
4648 |
|
MYO7B |
|
-0.080565005 |
none |
|
|
|
Poliovirus vaccine production |
Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game |
van der Sanden et al. |
2015 |
26581994 |
Cell line |
HEp-2C |
Infection with Attenuated Poliovirus |
ELISA |
Dharmacon |
Genome-wide |
siRNA |
Z-score |
> |
For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol |
|
4648 |
XM_290734 |
MYO7B |
|
36.181 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_292982 |
MYO7B |
|
17.942 |
Decreased viability |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_064265 |
MYO7B |
|
55.285 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_036115 |
MYO7B |
|
26.228 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_291001 |
MYO7B |
|
40.675 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_211339 |
MYO7B |
|
39.812 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_209429 |
MYO7B |
|
30.828 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_210411 |
MYO7B |
|
47.335 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_211291 |
MYO7B |
|
56.427 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4648 |
XM_211287 |
MYO7B |
|
52.21 |
none |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
GR00386-A-1 |
4648 |
4648 |
MYO7B |
|
110.846993683609 |
none |
|
|
|
NOD2 stimulation by MDP |
A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. |
Warner et al. |
2014 |
25170077 |
Cell line |
HEK293 stably expressing NOD2 |
Viability |
Luminescence |
Dharmacon |
Genome-wide |
siRNA |
Percentage growth |
Decreased: <70, increased: >120 |
Reagent IDs not provided |
GR00386-A-2 |
4648 |
4648 |
MYO7B |
|
-794.7 |
Increased IL-8 secretion |
|
|
|
MDP-induced IL-8 secretion |
A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. |
Warner et al. |
2014 |
25170077 |
Cell line |
HEK293 stably expressing NOD2 |
IL-8 secretion |
ELISA |
Dharmacon |
Genome-wide |
siRNA |
Percent inhibition of IL-8 secretion |
Increased: <-300, Decreased: >60 |
Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided |