RNAi

Gene Info

  • Species: Human (Homo sapiens)
  • GeneID: 6176
  • Symbol: RPLP1
  • Description: ribosomal protein lateral stalk subunit P1
DataSource: http://genomernai.dkfz.de/v16/genedetails/6176

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00053-A 6176 NM_001003 RPLP1 np sp none no Genome stability A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. Paulsen et al. 2009 19647519 Cell line HeLa gamma-H2AX phosphorylation and DNA content Fluorescence siARRAY human genome siRNA library Genome-wide siRNA p-value Complex criteria Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A 6176 NM_001003 RPLP1 np 0.987 none no Combinatorial effect with paclitaxel Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Whitehurst et al. 2007 17429401 Cell line NCI-H1155 Viability (synthetic lethal) ATP level # G-005000-01 Genome-wide siRNA Paclitaxel/control ratio Complex criteria Additional information about 87 high-confidence hits
GR00056-A 6176 NM_001003 RPLP1 np 0.887 none no Melanogenesis Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. Ganesan et al. 2008 19057677 Cell line MNT-1 Melanin protein expression and viability Absorbance and luminescence rp Genome-wide siRNA Normalized absorbance ratio > 2 standard deviations below mean Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1 6176 NM_001003 RPLP1 M-011135-00 -1.06 none no Wnt/beta-catenin pathway regulation (1) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Z-score > 4 Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2 6176 NM_001003 RPLP1 M-011135-00 sp none yes excluded from hits: Reagent targets ribosomal gene Wnt/beta-catenin pathway regulation (2) A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. Tang et al. 2008 18621708 Cell line HeLa Wnt pathway reporter Luminescence Human siArray siRNA library Genome-wide siRNA Complex, SP Complex criteria Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00098-A-1 6176 ENSG00000137818 RPLP1 ENSG00000137818 sp Increased S DNA content, increased G2M DNA content yes S arrest, validated with resynthesized esiRNA Cell division (1) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell number and DNA content Laser scanning cytometry rp Genome-wide esiRNA Complex, sp Complex criteria
GR00098-A-3 6176 ENSG00000137818 RPLP1 ENSG00000137818 -3.6 none yes Cell division (3) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Histone H3 phosphorylation; alpha-tubulin and pericentrin protein expression Fluorescence rp Selected genes esiRNA Mitotic index >
GR00098-A-4 6176 ENSG00000137818 RPLP1 ENSG00000137818 -1.1 none no Cell division (4) Genome-scale RNAi profiling of cell division in human tissue culture cells. Kittler et al. 2007 17994010 Cell line HeLa Cell size (forward scatter) Flow cytometry rp Selected genes esiRNA Cell size >
GR00151-A-1 6176 ENSG00000137818 RPLP1 np -1.719 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. SÅ‚abicki et al. 2010 20613862 Cell line HeLa (HR-DSBR) DR-GFP reporter Flow cytometry Custom-made Genome-wide esiRNA Z-score < -2 OR > 2
GR00180-A-1 6176 6176 RPLP1 PL-50050 0.805 none no Hepatitis C virus replication (1) A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. Tai et al. 2009 19286138 Cell line Huh7/Rep-Feo HCV replicon RNA copy number Luminescence siARRAY Human Genome siRNA Library Genome-wide siRNA q-value Complex criteria
GR00184-A-1 6176 NM_001003 RPLP1 M-011135-00 1.31428619114441 none no Self-renewal and pluripotency in human embryonic stem cells (1) A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Chia et al. 2010 20953172 Cell line hESC H1 POU5F1 protein expression Fluorescence SMARTpool siRNA library Genome-wide siRNA Z-score < -2
GR00196-A-1 6176 ENSG00000137818 np sp Decreased viability of wild-type and TP53 knockout cells no viability Z-score < -1.5 TP53 interactions (1) A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Krastev et al. 2011 21642980 Cell line HCT116 ( wildtype and TP53 knockout) TP53 protein expression and viability Fluorescence rp Genome-wide esiRNA Complex, sp Complex criteria
GR00197-A-1 6176 6176 RPLP1 M-011135-00 -1.862720768 none no Human papillomavirus oncogene expression regulation (1) Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. Smith et al. 2010 20133580 Cell line C33A/BE2/18LCR c4 HPV18 LCR reporter activity Luminescence Human siGENOME SMARTpool library Genome-wide siRNA Z-score > Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00209-A-1 6176 NM_001003, NM_213725 RPLP1 np 0.67 Nucleolar pre-40S maturation defects yes 10 nM siRNA; ratio nucleoplasmic/total hit classified cells: 0.0826945 Ribosome biogenesis (1) A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export. Wild et al. 2010 21048991 Cell line HeLa Rps2 protein expression Fluorescence np Selected genes siRNA Average relative rank from > > 0.2
GR00209-A-2 6176 NM_001003, NM_213725 RPLP1 np sp Nuclear 40S maturation defects yes Ribosome biogenesis (2) A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export. Wild et al. 2010 21048991 Cell line HeLa Enp1 protein expression Fluorescence np Selected genes siRNA Complex, sp Complex criteria
GR00209-A-3 6176 NM_001003, NM_213725 RPLP1 np 0.38 Nuclear 60S biogenesis defects no Ribosome biogenesis (3) A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export. Wild et al. 2010 21048991 Cell line HeLa Rpl29 protein expression Fluorescence np Selected genes siRNA Average relative rank from > > 0.25
GR00232-A-1 6176 NM_001003 RPLP1 v2HS_143168 1.010372837 Decreased ionizing radiation sensitivity no DNA damage regulation after ionizing radiation (1) A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Hurov et al. 2010 20810650 Cell line U2OS Ionizing radiation sensitivity Fluorescence pSMP Genome-wide shRNA log2 mean ratio < -1 OR > 1 The shRNA library is in a MSCV-PURO-based vector (MSCV-PM; Open Biosystems nomenclature is pSMP). The shRNAs with the “V3LHS” identifier were subcloned into the MSCV-PM vector from the pGIPZ vector. Additional information about a secondary screen in H460 cells.
GR00236-A-1 6176 6176 RPLP1 M-011135-00 1.27369176259159 none no Homologous recombination DNA double-strand break repair (HR-DSBR) (1) A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Adamson et al. 2012 22344029 Cell line DR-U2OS (HR-DSBR) DR-GFP reporter and DNA content Fluorescence Human siGENOME siRNA (G-005000-05) Genome-wide siRNA Relative HR ratio < ~0.4 OR > 1.88 Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1 6176 NM_001003 RPLP1 M-011135-00 5.92 Decreased viability yes TRAIL-induced apoptosis (1) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability Luminescence SMART-pool siRNA Genome-wide siRNA Z-score > 4 Author-submitted data
GR00240-S-2 6176 NM_001003 RPLP1 M-011135-00 np none no Z-score 3.296; viability Z-score > 4 TRAIL-induced apoptosis (2) A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. Kranz and Boutros 2014 24442637 Cell line U251MG Viability (synthetic lethal) Luminescence SMART-pool siRNA Genome-wide siRNA Differential score > 3.6 AND viability Z-score < 4 Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1 6176 NM_001003 RPLP1 np sp none no Selective autophagy regulation (1) Image-based genome-wide siRNA screen identifies selective autophagy factors. Orvedahl et al. 2011 22020285 Cell line HeLa/GFP-LC3 Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression Fluorescence siGenome Genome-wide siRNA Z-score Complex criteria
GR00247-A-1 6176 RPLP1 np sp none rank: 781 Regulation of FOXO1 nuclear localization (1) Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. Senapedis et al. 2011 21460183 Cell line U2OS EGFP-FOXO1a protein expression and DNA content Fluorescence Human Genome library Genome-wide siRNA Complex, sp Complex criteria
GR00249-S 6176 6176 RPLP1 s12239 -2.60093 Decreased vaccinia virus (VACV) infection no number of cells compared to control (%): 59.07 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 6176 6176 RPLP1 s194758 -2.84812 Decreased vaccinia virus (VACV) infection no number of cells compared to control (%): 57.03 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S 6176 6176 RPLP1 s194759 -2.85539 Decreased vaccinia virus (VACV) infection no number of cells compared to control (%): 53.49 Vaccinia virus (VACV) infection Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. Sivan et al. 2013 23401514 Cell line HeLa Vaccinia virus VACV IHD-J/GFP protein expression and DNA content Fluorescence Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus Genome-wide siRNA Z-score > Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00253-A 6176 NM_001003 RPLP1 np -3.32 Decreased hepcidin::fluc mRNA expression hepcidin regulation Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling. Mleczko-Sanecka et al. 2014 24385536 Cell line Huh7 hepcidin::fluc mRNA expression Luminescence siGenome siARRAY SMARTpool Genome-wide siRNA Z-score > Cutoff <
GR00255-A-1 6176 6176 RPLP1 TRCN0000117522, TRCN0000117523, TRCN0000117524, TRCN0000117525, TRCN0000117526 1.203212245 none Negative genetic interactions (1) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.0 HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2 6176 6176 RPLP1 TRCN0000117522, TRCN0000117523, TRCN0000117524, TRCN0000117525, TRCN0000117526 -0.117016929 none Negative genetic interactions (2) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.0 HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3 6176 6176 RPLP1 TRCN0000117522, TRCN0000117523, TRCN0000117524, TRCN0000117525, TRCN0000117526 -0.842811289 none Negative genetic interactions (3) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.2 HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4 6176 6176 RPLP1 TRCN0000117522, TRCN0000117523, TRCN0000117524, TRCN0000117525, TRCN0000117526 2.516638572 none Negative genetic interactions (4) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -1.2 HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5 6176 6176 RPLP1 TRCN0000117522, TRCN0000117523, TRCN0000117524, TRCN0000117525, TRCN0000117526 -0.609732913 none Negative genetic interactions (5) A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. Vizeacoumar et al. 2013 24104479 Cell line HCT116 shRNA abundance Microarray np Genome-wide shRNA differential Gene Activity Ranking Profile (dGARP) < -0.8 HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00290-A 6176 6176 RPLP1 SI00706825 1 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00706811 2 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00584857 2 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00706832 1 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00584843 1 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00584836 2 Decreased centriole number Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00290-A 6176 6176 RPLP1 SI00706818 0 none Centriole biogenesis Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells Balestra et al. 2013 23769972 Cell line HeLa Centriole number Fluorescence Qiagen Genome-wide siRNA Phenotype strength 0: none, 1: weak, 2: clear, 3: strong For alternative scores (RSA and Top2) see publication.
GR00293-A 6176 RPLP1 np 0.44 none Combinatorial effect with paclitaxel Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death Sinnott et al. 2014 24860162 Cell line HCC366 Viability Luminescence Thermo-Fisher Genome-wide siRNA Z-score < -2.5 Final hits according to the author are indicated in the comment.
GR00300-A 6176 RPLP1 TRCN0000117524, TRCN0000117523, TRCN0000117526, TRCN0000117525, TRCN0000117522 0 none Combinatorial effect with RAF inhibitor PLX4720 A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition. Whittaker et al. 2013 23288408 Cell line A375 shRNA abundance Sequencing TRC Genome-wide shRNA Number of shRNAs ranked Top1000 > 2 The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A 6176 NM_001003 RPLP1 np -2.71 none Clear cell renal cell carcinoma (ccRCC) survival regulation Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target Gerlinger et al. 2012 22362593 Cell line VHL-deficient RCC4 Proliferation and Viability Fluorescence np Genome-wide siRNA Z-score < In the phenotype data duplicates were in the original document, which have been removed.
GR00313-A 6176 NM_001003 RPLP1 np -2.31 none TNF-alpha pathway regulation A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. Nickles et al. 2012 22733992 Cell line HEK293T NFkappaB pathway reporter Luminescence Qiagen Genome-wide siRNA Z-score < Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%.
6176 6176 RPLP1 -0.16999999999999998 none
GR00376-A-1 6176 6176 RPLP1 0.926236325 none Mitigators of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2 6176 6176 RPLP1 8.53477E-5 Decreased viability with SS1P at EC30 Sensitizers of SS1P-induced immunotoxicity Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity Pasetto et al. 2015 25713356 Cell line KB cells Viability Luminescence Ambion Silencer Select Version 4 Genome-wide siRNA RSA P-value <0.001 SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
6176 NM_001003 RPLP1 20.491 Decreased viability