RNAi

Gene Info

• Species: Human (Homo sapiens)
• GeneID: 6217
• Symbol: RPS16
• Description: ribosomal protein S16

DataSource: http://genomernai.dkfz.de/v16/genedetails/6217

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00053-A	6217	NM_001020	RPS16	np	sp	Increased gamma-H2AX phosphorylation		no	group 4	Genome stability	A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability.	Paulsen et al.	2009	19647519	Cell line	HeLa	gamma-H2AX phosphorylation and DNA content	Fluorescence	siARRAY human genome siRNA library	Genome-wide	siRNA	p-value	Complex criteria	Confidence groupings from 4 to 1 (highest level of confidence in group 4)
GR00054-A	6217	NM_001020	RPS16	np	0.953	none		no		Combinatorial effect with paclitaxel	Synthetic lethal screen identification of chemosensitizer loci in cancer cells.	Whitehurst et al.	2007	17429401	Cell line	NCI-H1155	Viability (synthetic lethal)	ATP level	# G-005000-01	Genome-wide	siRNA	Paclitaxel/control ratio	Complex criteria	Additional information about 87 high-confidence hits
GR00056-A	6217	NM_001020	RPS16	np	1.124	none		no		Melanogenesis	Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells.	Ganesan et al.	2008	19057677	Cell line	MNT-1	Melanin protein expression and viability	Absorbance and luminescence	rp	Genome-wide	siRNA	Normalized absorbance ratio	> 2 standard deviations below mean	Additional information about a secondary screen (retest to determine false-positive rate)
GR00057-A-1	6217	XM_371151	LOC388519	M-031255-00	0.26	none		no		Wnt/beta-catenin pathway regulation (1)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Z-score	> 4	Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-1	6217	NM_001020	RPS16	M-013627-00	-0.45	none		no		Wnt/beta-catenin pathway regulation (1)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Z-score	> 4	Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2	6217	XM_371151	LOC388519	M-031255-00	sp	none		no		Wnt/beta-catenin pathway regulation (2)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Complex, SP	Complex criteria	Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00057-A-2	6217	NM_001020	RPS16	M-013627-00	sp	none		no		Wnt/beta-catenin pathway regulation (2)	A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.	Tang et al.	2008	18621708	Cell line	HeLa	Wnt pathway reporter	Luminescence	Human siArray siRNA library	Genome-wide	siRNA	Complex, SP	Complex criteria	Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries).
GR00095-A	6217	NM_001020	RPS16	np	np	none				p53 pathway components	A large-scale RNAi screen in human cells identifies new components of the p53 pathway	Berns et al.	2004	15042092	Cell line	BJ-TERT-tsLT fibroblasts	Proliferation	Colony-forming cell assay	Nki library	Genome-wide	shRNA	np	np
GR00098-A-1	6217	ENSG00000105193	RPS16	ENSG00000105193	sp	Increased S DNA content		yes	G2 arrest, validated with resynthesized esiRNA	Cell division (1)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Cell number and DNA content	Laser scanning cytometry	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00098-A-2	6217	ENSG00000105193	RPS16	ENSG00000105193_2	sp	G2 arrest		yes		Cell division (2)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Cell number and DNA content	Laser scanning cytometry	rp	Selected genes	esiRNA	Complex, sp	Complex criteria
GR00098-A-3	6217	ENSG00000105193	RPS16	ENSG00000105193	-1	none		yes		Cell division (3)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Histone H3 phosphorylation; alpha-tubulin and pericentrin protein expression	Fluorescence	rp	Selected genes	esiRNA	Mitotic index	>
GR00098-A-4	6217	ENSG00000105193	RPS16	ENSG00000105193	-4.4	none		no		Cell division (4)	Genome-scale RNAi profiling of cell division in human tissue culture cells.	Kittler et al.	2007	17994010	Cell line	HeLa	Cell size (forward scatter)	Flow cytometry	rp	Selected genes	esiRNA	Cell size	>
GR00123-A	6217	NM_001020	RPS16	145752, 145753, 145751	np	none				Combinatorial effect with nutlin-3	An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors	Brummelkamp et al.	2006	16474381	Cell line	MCF-7	Viability	Fluoresence	Nki library	Genome-wide	shRNA	log2 ratio	np
GR00151-A-1	6217	ENSG00000105193	RPS16P10	np	-1.068	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.	SÅ‚abicki et al.	2010	20613862	Cell line	HeLa	(HR-DSBR) DR-GFP reporter	Flow cytometry	Custom-made	Genome-wide	esiRNA	Z-score	< -2 OR > 2
GR00165-A	6217	XR_040892, XR_040893, XR_040894, XM_001718374, NM_001020, XM_497657, XM_942704	RPS16	M-031255-00	np	Elongated cells		no		HeLa cell morphology	Clustering phenotype populations by genome-wide RNAi and multiparametric imaging.	Fuchs et al.	2010	20531400	Cell line	HeLa	Cell morphology	Fluorescence	siGENOME	Genome-wide	siRNA	Complex, sp	np
GR00180-A-1	6217	6217	RPS16	PL-50024	3.3e-05	Decreased Hepatitis C virus replication		no		Hepatitis C virus replication (1)	A functional genomic screen identifies cellular cofactors of hepatitis C virus replication.	Tai et al.	2009	19286138	Cell line	Huh7/Rep-Feo	HCV replicon RNA copy number	Luminescence	siARRAY Human Genome siRNA Library	Genome-wide	siRNA	q-value	Complex criteria
GR00184-A-1	6217	NM_001020	RPS16	M-013627-00	0.386244312181017	none		no	top 200 hESCs survivability candidate gene (nuclei number reduction)	Self-renewal and pluripotency in human embryonic stem cells (1)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	SMARTpool siRNA library	Genome-wide	siRNA	Z-score	< -2
GR00184-A-1	6217	XM_371151	LOC388519	M-031255-00	1.91920971696256	none		no	top 200 hESCs survivability candidate gene (nuclei number reduction)	Self-renewal and pluripotency in human embryonic stem cells (1)	A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.	Chia et al.	2010	20953172	Cell line	hESC H1	POU5F1 protein expression	Fluorescence	SMARTpool siRNA library	Genome-wide	siRNA	Z-score	< -2
GR00195-A-1	6217	6217	RPS16	np	sp	Decreased influenza A/WSN/33 replication		yes		Influenza virus infection (1)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and 293T	Number of influenza A H1N1 (A/WSN/33) infected cells and viral polymerase protein expression	Fluorescence and luminescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Genome-wide	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00195-A-2	6217	6217	RPS16	Hs_RPS16_5, Hs_RPS16_8, Hs_RPS16_7, Hs_RPS16_6	sp	Decreased influenza A H1N1 (A/WSN/33) virus numbers		yes		Influenza virus infection (2)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and MDCK	Number of influenza A H1N1 (A/WSN/33) viruses	Fluorescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Selected genes	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00195-A-3	6217	6217	RPS16	Hs_RPS16_5, Hs_RPS16_8, Hs_RPS16_7, Hs_RPS16_6	sp	none		no		Influenza virus infection (3)	Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication.	Karlas et al.	2010	20081832	Cell line	A549 and MDCK	Number of influenza A H1N1 (A/Hamburg/04/2009) viruses	Fluorescence	Hu_Genome 1.0 and Human Druggable Genome siRNA Set V2.0	Selected genes	siRNA	Complex, sp	Complex criteria	Additional information about a secondary screen with influenza A H5N1 strain (A/Vietnam/1203/2004)
GR00196-A-1	6217	ENSG00000105193		np	sp	Decreased viability of wild-type and TP53 knockout cells		no	viability Z-score < -2	TP53 interactions (1)	A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.	Krastev et al.	2011	21642980	Cell line	HCT116 ( wildtype and TP53 knockout)	TP53 protein expression and viability	Fluorescence	rp	Genome-wide	esiRNA	Complex, sp	Complex criteria
GR00197-A-1	6217	388519	LOC388519	M-031255-00	-3.618429074	none		no		Human papillomavirus oncogene expression regulation (1)	Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.	Smith et al.	2010	20133580	Cell line	C33A/BE2/18LCR c4	HPV18 LCR reporter activity	Luminescence	Human siGENOME SMARTpool library	Genome-wide	siRNA	Z-score	>	Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00197-A-1	6217	6217	RPS16	M-013627-00	-1.966613586	none		no		Human papillomavirus oncogene expression regulation (1)	Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.	Smith et al.	2010	20133580	Cell line	C33A/BE2/18LCR c4	HPV18 LCR reporter activity	Luminescence	Human siGENOME SMARTpool library	Genome-wide	siRNA	Z-score	>	Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5
GR00209-A-1	6217	NM_001020	RPS16	np	0.49	Nucleolar pre-40S maturation defects		yes	10 nM siRNA; ratio nucleoplasmic/total hit classified cells: 0.232064	Ribosome biogenesis (1)	A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export.	Wild et al.	2010	21048991	Cell line	HeLa	Rps2 protein expression	Fluorescence	np	Selected genes	siRNA	Average relative rank from >	> 0.2
GR00209-A-2	6217	NM_001020	RPS16	np	sp	Nuclear 40S maturation defects		yes		Ribosome biogenesis (2)	A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export.	Wild et al.	2010	21048991	Cell line	HeLa	Enp1 protein expression	Fluorescence	np	Selected genes	siRNA	Complex, sp	Complex criteria
GR00209-A-3	6217	NM_001020	RPS16	np	np	none		no		Ribosome biogenesis (3)	A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export.	Wild et al.	2010	21048991	Cell line	HeLa	Rpl29 protein expression	Fluorescence	np	Selected genes	siRNA	Average relative rank from >	> 0.25
GR00236-A-1	6217	388519	LOC388519	M-031255-00	0.671358316633972	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response.	Adamson et al.	2012	22344029	Cell line	DR-U2OS	(HR-DSBR) DR-GFP reporter and DNA content	Fluorescence	Human siGENOME siRNA (G-005000-05)	Genome-wide	siRNA	Relative HR ratio	< ~0.4 OR > 1.88	Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00236-A-1	6217	6217	RPS16	M-013627-00	0.717065480313265	none		no		Homologous recombination DNA double-strand break repair (HR-DSBR) (1)	A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response.	Adamson et al.	2012	22344029	Cell line	DR-U2OS	(HR-DSBR) DR-GFP reporter and DNA content	Fluorescence	Human siGENOME siRNA (G-005000-05)	Genome-wide	siRNA	Relative HR ratio	< ~0.4 OR > 1.88	Cutoff values correspond 2 standard deviations from the screen-wide mean
GR00240-S-1	6217	NM_001020	RPS16	M-013627-00	0.36	none		yes		TRAIL-induced apoptosis (1)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Z-score	> 4	Author-submitted data
GR00240-S-1	6217	XM_371151	LOC388519	M-031255-00	7.59	Decreased viability		yes		TRAIL-induced apoptosis (1)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Z-score	> 4	Author-submitted data
GR00240-S-2	6217	NM_001020	RPS16	M-013627-00	0.2	none		no	Z-score 0.68	TRAIL-induced apoptosis (2)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability (synthetic lethal)	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Differential score	> 3.6 AND viability Z-score < 4	Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00240-S-2	6217	XM_371151	LOC388519	M-031255-00	np	none		no	Z-score 4.004; viability Z-score > 4	TRAIL-induced apoptosis (2)	A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis.	Kranz and Boutros	2014	24442637	Cell line	U251MG	Viability (synthetic lethal)	Luminescence	SMART-pool siRNA	Genome-wide	siRNA	Differential score	> 3.6 AND viability Z-score < 4	Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen.
GR00242-A-1	6217	NM_001020	RPS16	np	sp	none		no		Selective autophagy regulation (1)	Image-based genome-wide siRNA screen identifies selective autophagy factors.	Orvedahl et al.	2011	22020285	Cell line	HeLa/GFP-LC3	Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression	Fluorescence	siGenome	Genome-wide	siRNA	Z-score	Complex criteria
GR00246-A	6217		RPS16	np	np	none		no		Telomere protection	A siRNA-based screen for genes involved in chromosome end protection.	Lackner et al.	2011	21760879	Cell line	HeLa	Telomere dysfunction-induced foci (TIF) formation (53BP1 protein expression, FITC-TelC and DNA content)	Fluorescence	siGENOME	Selected genes	siRNA	Number of TIFs	>	HeLa 1.2.11 cells used
GR00247-A-1	6217		RPS16	np	sp	none			rank: 350; excluded (false positive)	Regulation of FOXO1 nuclear localization (1)	Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain.	Senapedis et al.	2011	21460183	Cell line	U2OS	EGFP-FOXO1a protein expression and DNA content	Fluorescence	Human Genome library	Genome-wide	siRNA	Complex, sp	Complex criteria
GR00249-S	6217	6217	RPS16	J-013627-05	-1.82375	Decreased viability		no	number of cells compared to control (%): 41.64	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	6217	6217	RPS16	M-013627-01	-1.53298	Decreased viability		no	number of cells compared to control (%): 39.93	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	6217	6217	RPS16	s12314	-2.82137	Decreased vaccinia virus (VACV) infection		no	number of cells compared to control (%): 53.89	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	6217	6217	RPS16	s12315	-2.80245	Decreased viability		no	number of cells compared to control (%): 48.18	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00249-S	6217	6217	RPS16	s12316	-2.36005	Decreased viability		no	number of cells compared to control (%): 49.93	Vaccinia virus (VACV) infection	Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis.	Sivan et al.	2013	23401514	Cell line	HeLa	Vaccinia virus VACV IHD-J/GFP protein expression and DNA content	Fluorescence	Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus	Genome-wide	siRNA	Z-score	>	Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %.
GR00253-A	6217	NM_001020	RPS16	np	-0.612	none				hepcidin regulation	Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling.	Mleczko-Sanecka et al.	2014	24385536	Cell line	Huh7	hepcidin::fluc mRNA expression	Luminescence	siGenome siARRAY SMARTpool	Genome-wide	siRNA	Z-score	>	Cutoff <
GR00255-A-1	6217	6217	RPS16	TRCN0000005471, TRCN0000005472, TRCN0000005473, TRCN0000005474	0.604020059	none				Negative genetic interactions (1)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-2	6217	6217	RPS16	TRCN0000005471, TRCN0000005472, TRCN0000005473, TRCN0000005474	0.774137086	none				Negative genetic interactions (2)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.0	HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-3	6217	6217	RPS16	TRCN0000005471, TRCN0000005472, TRCN0000005473, TRCN0000005474	1.072747504	none				Negative genetic interactions (3)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-4	6217	6217	RPS16	TRCN0000005471, TRCN0000005472, TRCN0000005473, TRCN0000005474	0.403762674	none				Negative genetic interactions (4)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -1.2	HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00255-A-5	6217	6217	RPS16	TRCN0000005471, TRCN0000005472, TRCN0000005473, TRCN0000005474	-1.157646968	Negative genetic interaction between KRASG13D/+ and KRAS+/-				Negative genetic interactions (5)	A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.	Vizeacoumar et al.	2013	24104479	Cell line	HCT116	shRNA abundance	Microarray	np	Genome-wide	shRNA	differential Gene Activity Ranking Profile (dGARP)	< -0.8	HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells)
GR00293-A	6217		RPS16	np	0.449	none				Combinatorial effect with paclitaxel	Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death	Sinnott et al.	2014	24860162	Cell line	HCC366	Viability	Luminescence	Thermo-Fisher	Genome-wide	siRNA	Z-score	< -2.5	Final hits according to the author are indicated in the comment.
GR00300-A	6217		RPS16	TRCN0000005474, TRCN0000005473, TRCN0000005472, TRCN0000005471	0	none				Combinatorial effect with RAF inhibitor PLX4720	A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition.	Whittaker et al.	2013	23288408	Cell line	A375	shRNA abundance	Sequencing	TRC	Genome-wide	shRNA	Number of shRNAs ranked Top1000	> 2	The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors.
GR00303-A	6217	NM_001020	RPS16	np	-3.15	Decreased cell number				Clear cell renal cell carcinoma (ccRCC) survival regulation	Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target	Gerlinger et al.	2012	22362593	Cell line	VHL-deficient RCC4	Proliferation and Viability	Fluorescence	np	Genome-wide	siRNA	Z-score	<	In the phenotype data duplicates were in the original document, which have been removed.
GR00310-A-1	6217	6217	RPS16	np	-1.31	none				Sindbis virus (SINV) infection (1)	Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry	Ooi et al.	2013	24367265	Cell line	U2OS	Sindbis virus (SINV) reporter	Luminescence	Ambion Silencer V3	Genome-wide	siRNA	Z-score	< -3 OR > 2
	6217	6217	RPS16	np	85.9	Inconclusive			47,2% viability
GR00318-A	6217	6217	RPS16	np	0.941	none			siRNA set: druggable genome	Huntingtin toxicity	A Genome-Scale RNA–Interference Screen Identifies RRAS Signaling as a Pathologic Feature of Huntington’s Disease	Miller et al.	2012	23209424	Cell line	HEK293T	Caspase 3/7 activity	Fluorescence	Dharmacon	Selected genes	siRNA	Sum of normalized caspase 3/7 activity mean and standard error	< 0.683	HEK293T cells were cotransfected with mutant Huntingtin fused to GFP (Htt1-558141Q-GFP).
GR00343-S	6217	6217	RPS16	TRCN0000005473	-1.2074999999999996	shRNA abundance <= 50%				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	6217	6217	RPS16	TRCN0000005472	0.7200000000000024	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	6217	6217	RPS16	TRCN0000005474	-0.09999999999999787	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
GR00343-S	6217	6217	RPS16	TRCN0000005471	-0.597500000000001	none				Lentiviral shRNA "Achilles Heel" screen	Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia	Cole et al.	2015	26058080	Cell line	K562	shRNA abundance	Fluorescence	Lentiviral shRNA library	Genome-wide	shRNA	Log2 ratio	<	Comparison of day 21 vs. day 0 abundance data
	6217	6217	RPS16		0	none
GR00376-A-1	6217	6217	RPS16		0.666672698	none				Mitigators of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID
GR00376-A-2	6217	6217	RPS16		4.24604E-4	Decreased viability with SS1P at EC30				Sensitizers of SS1P-induced immunotoxicity	Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity	Pasetto et al.	2015	25713356	Cell line	KB cells	Viability	Luminescence	Ambion Silencer Select Version 4	Genome-wide	siRNA	RSA P-value	<0.001	SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID
GR00378-A	6217		RPS16		-1.288279174	none				Poliovirus vaccine production	Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game	van der Sanden et al.	2015	26581994	Cell line	HEp-2C	Infection with Attenuated Poliovirus	ELISA	Dharmacon	Genome-wide	siRNA	Z-score	>	For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol
	6217	NM_001020	RPS16		26.404	none
GR00386-A-1	6217	6217	RPS16		45.2714989780758	Decreased viability				NOD2 stimulation by MDP	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	Viability	Luminescence	Dharmacon	Genome-wide	siRNA	Percentage growth	Decreased: <70, increased: >120	Reagent IDs not provided
GR00386-A-2	6217	6217	RPS16		-90.6	none				MDP-induced IL-8 secretion	A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion.	Warner et al.	2014	25170077	Cell line	HEK293 stably expressing NOD2	IL-8 secretion	ELISA	Dharmacon	Genome-wide	siRNA	Percent inhibition of IL-8 secretion	Increased: <-300, Decreased: >60	Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided