RNAi

Gene Info

  • Species: Fly (Drosophila melanogaster)
  • GeneID: 33235
  • Symbol: Sf3b1
  • Description: Splicing factor 3b subunit 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/33235

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 33235 FBgn0031266 CG2807 DRSC00535 0.019502901 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 33235 FBgn0031266 CG2807 DRSC00535 -2.688185462 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00004-A-0 33235 CG2807 DRSC00535 0.93 siRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00004-A-0 33235 CG2807 DRSC00535 0.43 miRNA reporter downregulated no Drosophila small RNA pathways Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Zhou et al. 2008 19026789 Cell line S2 miRNA and siRNA pathway activity Dual luciferase DRSC dsRNA Fold change endo-siRNA reporter 1.5/0.6
GR00010-A 33235 CG2807 np np Increased poly-(A+) mRNA nuclear accumulation yes mRNA nuclear export Definition of global and transcript-specific mRNA export pathways in metazoans. Farny et al. 2008 18086857 Cell line S2R+ Poly-(A+) mRNA nuclear accumulation Fluorescence Full genome dsRNA library Genome-wide dsRNA Visual inspection np
GR00021-A-0 33235 FBgn0031266 DRSC00535 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 33235 CG2807 HFA00535 4.3 Decreased cell number and viability yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 33235 CG2807 HFA00535 6.1 Decreased cell number and viability no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
33235 FBgn0031266 CG2807 DRSC00535 weak Binucleate cells with decreased viability no
GR00036-A 33235 CG2807 CG2807 DRSC00535 moderate Decreased GFP protein expression yes decreased intracellular infection Intracellular Listeria monocytogenes infection Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Agaisse et al. 2005 16020693 Cell line SL2 GFP protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np
GR00045-A-0 33235 FBgn0031266 DRSC00535 -3.56 Decreased Hedgehog reporter activity no Hedgehog signaling A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken et al. 2005 16311596 Cell line Clone 8 Hedgehog signaling Dual luciferase DRSC dsRNA Z-score >
GR00047-A-1 33235 FBgn0031266 CG2807 DRSC00535 sp Decreased horseradish peroxidase protein expression no Constitutive protein secretion and Golgi organization (1) Functional genomics reveals genes involved in protein secretion and Golgi organization. Bard et al. 2006 16452979 Cell line S2 Horseradish peroxidase protein expression Luminescence Genome-wide dsRNA Z-score < -1.5
33235 FBgn0031266 CG2807 RE14559 sp none no library: DGC2
33235 FBgn0031266 CG2807 RH74732 sp none no library: DGC2
GR00051-A-1 33235 CG2807 CG2807 np np none no Candida albicans phagocytosis (1) Identification of Drosophila gene products required for phagocytosis of Candida albicans. Stroschein-Stevenson et al. 2006 16336044 Cell line S2 C. albicans phagocytosis, actin protein expression and DNA content Fluorescence rp Selected genes dsRNA Percentage cells phagocytosing C. albicans <
GR00062-A 33235 FBgn0031266 CG2807 np 6 Decreased Ca2+ influx final hit Ca2+ channel regulation CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Vig et al. 2006 16645049 Cell line S2R+ Ca2+ influx Fluorescence np Genome-wide dsRNA Complex, sp >
GR00092-A 33235 FBgn0031266 CG2807 RDM1190-7299229 np none no Microtubule assembly and organization Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Ducat et al. 2008 18463163 Cell line S2 DNA content, alpha-tubulin and CP309 protein expression Fluorescence OpenBiosystems Drosophila RNAi Library 1.0 Mitotic regulator genes dsRNA Complex, sp Complex criteria
GR00130-A 33235 FBgn0031266 CG2807 np <= -2 Decreased mutant human huntingtin aggregation no Mutant human huntingtin aggregation RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. Doumanis et al. 2009 19789644 Cell line BG2-c2 Nhtt(62Q)EGFP aggregate number and size Fluorescence OpenBiosystems RNAi library Selected genes dsRNA Z-score Suppressor: <
GR00131-A-1 33235 CG2807 DRSC00535 > 75 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 33235 CG2807 CG2807 25162 np Lethal yes early larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 33235 CG2807 CG2807 25161 np Lethal yes late larval Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-3 33235 CG2807 CG2807 25162 np Spotty (discontinuous) Z-line no Muscle morphogenesis and function (3) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 ZCL0663 protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 33235 CG2807 CG2807 25162 0.2 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 33235 CG2807 25162 0.14 Pupal lethal no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 33235 CG2807 CG2807 25161 -0.48235294117647 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 33235 CG2807 25161 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 33235 CG2807 25162 sp Completely lethal (before pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00170-A-1 33235 CG2807 DRSC00535 -7.2 none no decreased cell viability or Sanger collection sequence HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00175-A 33235 CG2807 CG2807 DRSC31841 -0.412032698656824 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00175-A 33235 CG2807 CG2807 DRSC31840 -0.511180432995426 Downregulation of Hh pathway no Hh pathway regulation A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. DasGupta et al. 2007 17903264 Cell line rp rp Luminescence DRSC-v Selected genes dsRNA Average fractional change Complex criteria
GR00189-A-1 33235 FBgn0031266 CG2807 BKN21741 np Upregulation of Notch pathway no Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00214-A-1 33235 FBgn0031266 CG2807 DRSC00535 -4.54 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 33235 FBgn0031266 CG2807 DRSC00535 -1.8 Decreased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 33235 FBgn0031266 CG2807 DRSC00535 -5.85 Decreased ERK phosphorylation after sSpitz and EGF stimulation 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 33235 FBgn0031266 CG2807 DRSC00535 -4.35 Decreased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 33235 CG2807 AMB19042 1.68 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 33235 CG2807 2807R np Decreased Chp protein expression no not detectable Chp level Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00273-A-1 33235 FBgn0031266 CG2807 DRSC00535 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00282-A 33235 CG2807 CG2807 25162 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 33235 CG2807 CG2807 25161 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 33235 CG2807 CG2807 110091 -1.4174011279807093 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 33235 CG2807 CG2807 np 3 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 33235 FBgn0031266 CG2807 DRSC00535 np Actin defect, other cellular phenotype cell shape variable, decreased level of actin, increased nuclear actin, decreased cell number, loss of cell monolayer Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 33235 CG2807 CG2807 110091 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00326-A 33235 FBgn0031266 CG2807 110091 np Synaptic undergrowth, synaptic retractions Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 33235 FBgn0031266 CG2807 HM05176 np Agametic Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 33235 FBgn0031266 CG2807 HMS00055 np F1 lethal F1 larval lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
33235 FBgn0031266 np np sp none
GR00379-A-1 33235 CG2807 110091 4.352132618557336 High decrease in nos and yTub37c expression No eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 33235 CG2807 110091 np No germline, 100% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 33235 CG2807 BL33650 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 33235 CG2807 KK110091 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 33235 CG2807 BL28965 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 33235 CG2807 GD25162 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.