Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:8092
• Symbol:ALX1
• Description:ALX homeobox 1

DataSource: http://genomecrispr.dkfz.de/#!/results/ALX1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
85280345	85280368	12	+	26472758	Jiyoye	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.6179424777226942	[177]	[205]	4	hSpCas9	negative selection	5738
85283684	85283707	12	+	26472758	Jiyoye	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.1441409756720794	[179]	[149]	1	hSpCas9	negative selection	5739
85283725	85283748	12	+	26472758	Jiyoye	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-3.0522562371314237	[54]	[4]	-9	hSpCas9	negative selection	5740
85280431	85280454	12	-	26472758	Jiyoye	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	2.0479256604127762	[92]	[289]	8	hSpCas9	negative selection	5741
85280466	85280489	12	+	26472758	Jiyoye	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.7387968725970798	[88]	[111]	5	hSpCas9	negative selection	5742
85283849	85283872	12	+	26472758	Jiyoye	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	1.452690040758242	[77]	[160]	8	hSpCas9	negative selection	5743
85286960	85286983	12	+	26472758	Jiyoye	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.031113849485032485	[352]	[271]	0	hSpCas9	negative selection	5744
85280313	85280336	12	+	26472758	Jiyoye	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	-0.5009021290530874	[151]	[80]	-4	hSpCas9	negative selection	5745
85283663	85283686	12	+	26472758	Jiyoye	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	1.2324968500929263	[56]	[100]	7	hSpCas9	negative selection	5746
85280393	85280416	12	+	26472758	Jiyoye	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.0009617326140355931	[162]	[122]	0	hSpCas9	negative selection	5747
85280345	85280368	12	+	26472758	KBM7	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.5750419225063023	[836,583]	[735,271]	6	hSpCas9	negative selection	196856
85283684	85283707	12	+	26472758	KBM7	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.14162828545811343	[489,474]	[324,184]	1	hSpCas9	negative selection	196857
85283725	85283748	12	+	26472758	KBM7	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-1.6139276628846821	[63,68]	[13,6]	-8	hSpCas9	negative selection	196858
85280431	85280454	12	-	26472758	KBM7	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.746851430292079	[934,812]	[875,519]	7	hSpCas9	negative selection	196859
85280466	85280489	12	+	26472758	KBM7	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.0463565614483203	[569,388]	[201,253]	0	hSpCas9	negative selection	196860
85283849	85283872	12	+	26472758	KBM7	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5734828520899065	[587,307]	[399,219]	6	hSpCas9	negative selection	196861
85286960	85286983	12	+	26472758	KBM7	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.7319930635242116	[1260,1351]	[1143,925]	7	hSpCas9	negative selection	196862
85280313	85280336	12	+	26472758	KBM7	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.3653327688818281	[413,387]	[228,255]	4	hSpCas9	negative selection	196863
85283663	85283686	12	+	26472758	KBM7	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.0424640979443881	[708,651]	[388,240]	0	hSpCas9	negative selection	196864
85280393	85280416	12	+	26472758	KBM7	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.311774140999419	[810,755]	[356,547]	3	hSpCas9	negative selection	196865
85280345	85280368	12	+	26472758	Raji	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.6121238376006755	[264]	[93]	6	hSpCas9	negative selection	387974
85283684	85283707	12	+	26472758	Raji	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.785455440486237	[269]	[107]	7	hSpCas9	negative selection	387975
85283725	85283748	12	+	26472758	Raji	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.3780432917966423	[27]	[4]	-3	hSpCas9	negative selection	387976
85280431	85280454	12	-	26472758	Raji	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.26913460537021183	[353]	[98]	2	hSpCas9	negative selection	387977
85280466	85280489	12	+	26472758	Raji	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.2086668712107812	[219]	[58]	2	hSpCas9	negative selection	387978
85283849	85283872	12	+	26472758	Raji	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.7398744061773413	[208]	[80]	7	hSpCas9	negative selection	387979
85286960	85286983	12	+	26472758	Raji	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	-0.19284948841444383	[659]	[133]	-2	hSpCas9	negative selection	387980
85280313	85280336	12	+	26472758	Raji	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.8272756161808642	[237]	[97]	7	hSpCas9	negative selection	387981
85283663	85283686	12	+	26472758	Raji	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.019288268114828977	[344]	[78]	0	hSpCas9	negative selection	387982
85280393	85280416	12	+	26472758	Raji	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.27836973218622235	[301]	[84]	2	hSpCas9	negative selection	387983
85280345	85280368	12	+	26472758	K562	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.8529359563664998	[485]	[740]	7	hSpCas9	negative selection	579092
85283684	85283707	12	+	26472758	K562	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.2503314205363384	[486]	[488]	2	hSpCas9	negative selection	579093
85283725	85283748	12	+	26472758	K562	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.2902224172795713	[61]	[63]	2	hSpCas9	negative selection	579094
85280431	85280454	12	-	26472758	K562	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	1.2942169607845981	[540]	[1119]	9	hSpCas9	negative selection	579095
85280466	85280489	12	+	26472758	K562	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.5284767236477843	[392]	[229]	-3	hSpCas9	negative selection	579096
85283849	85283872	12	+	26472758	K562	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.38481777680558726	[361]	[398]	3	hSpCas9	negative selection	579097
85286960	85286983	12	+	26472758	K562	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.5273792895430383	[742]	[903]	4	hSpCas9	negative selection	579098
85280313	85280336	12	+	26472758	K562	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.01963179118961645	[401]	[343]	0	hSpCas9	negative selection	579099
85283663	85283686	12	+	26472758	K562	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.06148232777920659	[648]	[524]	0	hSpCas9	negative selection	579100
85280393	85280416	12	+	26472758	K562	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.7755747846918093	[518]	[749]	6	hSpCas9	negative selection	579101
85280345	85280368	12	+	26627737	DLD1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.22503655314924892	[327]	[44,84,126]	-2	hSpCas9	negative selection	785733
85280469	85280492	12	-	26627737	DLD1	viability	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.16332983081424013	[868]	[382,163,351]	1	hSpCas9	negative selection	785734
85283650	85283673	12	+	26627737	DLD1	viability	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.16813516872955514	[1330]	[459,522,427]	1	hSpCas9	negative selection	785735
85283866	85283889	12	-	26627737	DLD1	viability	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.2968084008796834	[396]	[200,170,94]	3	hSpCas9	negative selection	785736
85301263	85301286	12	+	26627737	DLD1	viability	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.6407873539970763	[1177]	[621,365,704]	6	hSpCas9	negative selection	785737
85301264	85301287	12	+	26627737	DLD1	viability	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	1.0347288015796914	[591]	[321,554,282]	8	hSpCas9	negative selection	785738
85280345	85280368	12	+	26627737	GBM cells	viability after 5 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.3458002402038693	[167]	[93,134]	5	hSpCas9	negative selection	868048
85280469	85280492	12	-	26627737	GBM cells	viability after 5 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.08665905447903455	[397]	[196,204]	-1	hSpCas9	negative selection	868049
85283650	85283673	12	+	26627737	GBM cells	viability after 5 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.33616265325468797	[708]	[468,490]	5	hSpCas9	negative selection	868050
85283866	85283889	12	-	26627737	GBM cells	viability after 5 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	-0.19666378509654542	[311]	[170,120]	-3	hSpCas9	negative selection	868051
85301263	85301286	12	+	26627737	GBM cells	viability after 5 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.22900360003505105	[659]	[360,242]	-4	hSpCas9	negative selection	868052
85301264	85301287	12	+	26627737	GBM cells	viability after 5 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.14491820658304289	[400]	[197,190]	-2	hSpCas9	negative selection	868053
85280345	85280368	12	+	26627737	GBM cells	viability after 13 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.3837117556665538	[167]	[108,126]	5	hSpCas9	negative selection	950363
85280469	85280492	12	-	26627737	GBM cells	viability after 13 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.21096573166694432	[397]	[287,218]	2	hSpCas9	negative selection	950364
85283650	85283673	12	+	26627737	GBM cells	viability after 13 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.3061643479514409	[708]	[425,515]	4	hSpCas9	negative selection	950365
85283866	85283889	12	-	26627737	GBM cells	viability after 13 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	-0.43895822749138247	[311]	[152,101]	-5	hSpCas9	negative selection	950366
85301263	85301286	12	+	26627737	GBM cells	viability after 13 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.45651632779425566	[659]	[255,264]	-5	hSpCas9	negative selection	950367
85301264	85301287	12	+	26627737	GBM cells	viability after 13 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.0668038291023848	[400]	[215,200]	-1	hSpCas9	negative selection	950368
85280345	85280368	12	+	26627737	GBM cells	viability after 21 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.4897931640775685	[167]	[135,120]	5	hSpCas9	negative selection	1032678
85280469	85280492	12	-	26627737	GBM cells	viability after 21 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.030362429558134507	[397]	[229,194]	0	hSpCas9	negative selection	1032679
85283650	85283673	12	+	26627737	GBM cells	viability after 21 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.7407365262815713	[708]	[686,603]	7	hSpCas9	negative selection	1032680
85283866	85283889	12	-	26627737	GBM cells	viability after 21 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	-0.55661552472297	[311]	[132,98]	-5	hSpCas9	negative selection	1032681
85301263	85301286	12	+	26627737	GBM cells	viability after 21 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.2707446734296006	[659]	[303,290]	-2	hSpCas9	negative selection	1032682
85301264	85301287	12	+	26627737	GBM cells	viability after 21 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.23889832269360334	[400]	[254,258]	2	hSpCas9	negative selection	1032683
85280345	85280368	12	+	26627737	RPE1	viability after 9 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.00270239369804208	[752]	[295,158]	0	hSpCas9	negative selection	1114993
85280469	85280492	12	-	26627737	RPE1	viability after 9 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.38542030563624763	[1136]	[368,147]	-3	hSpCas9	negative selection	1114994
85283650	85283673	12	+	26627737	RPE1	viability after 9 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	-0.5540363855450363	[2619]	[583,525]	-4	hSpCas9	negative selection	1114995
85283866	85283889	12	-	26627737	RPE1	viability after 9 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.31241771604219915	[942]	[232,538]	2	hSpCas9	negative selection	1114996
85301263	85301286	12	+	26627737	RPE1	viability after 9 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.3082317067735163	[3558]	[681,1180]	-2	hSpCas9	negative selection	1114997
85301264	85301287	12	+	26627737	RPE1	viability after 9 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.2836616385067411	[1770]	[351,588]	-2	hSpCas9	negative selection	1114998
85280345	85280368	12	+	26627737	RPE1	viability after 12 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-1.005451799517505	[752]	[109,90]	-6	hSpCas9	negative selection	1197308
85280469	85280492	12	-	26627737	RPE1	viability after 12 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.11272511625970183	[1136]	[333,227]	0	hSpCas9	negative selection	1197309
85283650	85283673	12	+	26627737	RPE1	viability after 12 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	-0.7687427698581609	[2619]	[485,335]	-5	hSpCas9	negative selection	1197310
85283866	85283889	12	-	26627737	RPE1	viability after 12 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.07430327976042289	[942]	[206,329]	0	hSpCas9	negative selection	1197311
85301263	85301286	12	+	26627737	RPE1	viability after 12 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.0312386971967471	[3558]	[929,1026]	0	hSpCas9	negative selection	1197312
85301264	85301287	12	+	26627737	RPE1	viability after 12 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.254405439711048	[1770]	[275,528]	-2	hSpCas9	negative selection	1197313
85280345	85280368	12	+	26627737	RPE1	viability after 15 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-1.5116072403908447	[752]	[34,112]	-7	hSpCas9	negative selection	1279623
85280469	85280492	12	-	26627737	RPE1	viability after 15 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.40902934102603555	[1136]	[397,91]	-3	hSpCas9	negative selection	1279624
85283650	85283673	12	+	26627737	RPE1	viability after 15 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	-0.4560373053611322	[2619]	[636,446]	-3	hSpCas9	negative selection	1279625
85283866	85283889	12	-	26627737	RPE1	viability after 15 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.07147914986238285	[942]	[206,350]	0	hSpCas9	negative selection	1279626
85301263	85301286	12	+	26627737	RPE1	viability after 15 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.016482546670103293	[3558]	[830,1198]	0	hSpCas9	negative selection	1279627
85301264	85301287	12	+	26627737	RPE1	viability after 15 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.1457021485457286	[1770]	[513,592]	1	hSpCas9	negative selection	1279628
85280345	85280368	12	+	26627737	RPE1	viability after 18 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-1.1142142198607081	[752]	[60,128]	-6	hSpCas9	negative selection	1361938
85280469	85280492	12	-	26627737	RPE1	viability after 18 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.17685472355304	[1136]	[327,210]	-1	hSpCas9	negative selection	1361939
85283650	85283673	12	+	26627737	RPE1	viability after 18 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	-0.973791754843042	[2619]	[301,421]	-6	hSpCas9	negative selection	1361940
85283866	85283889	12	-	26627737	RPE1	viability after 18 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.027657499486360593	[942]	[183,339]	0	hSpCas9	negative selection	1361941
85301263	85301286	12	+	26627737	RPE1	viability after 18 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.0987813321480644	[3558]	[635,1447]	0	hSpCas9	negative selection	1361942
85301264	85301287	12	+	26627737	RPE1	viability after 18 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.0011732250620271056	[1770]	[312,654]	0	hSpCas9	negative selection	1361943
85280345	85280368	12	+	26627737	HeLa	viability after 8 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.6502804475432854	[485]	[107,31,249]	-6	hSpCas9	negative selection	1444253
85280469	85280492	12	-	26627737	HeLa	viability after 8 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.5356234100147883	[490]	[180,97,168]	-5	hSpCas9	negative selection	1444254
85283650	85283673	12	+	26627737	HeLa	viability after 8 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.12740415919501769	[790]	[463,317,365]	1	hSpCas9	negative selection	1444255
85283866	85283889	12	-	26627737	HeLa	viability after 8 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	-0.251181147764178	[456]	[215,113,180]	-2	hSpCas9	negative selection	1444256
85301263	85301286	12	+	26627737	HeLa	viability after 8 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.4959712712931059	[965]	[603,704,479]	4	hSpCas9	negative selection	1444257
85301264	85301287	12	+	26627737	HeLa	viability after 8 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.5599257136287064	[883]	[347,178,273]	-5	hSpCas9	negative selection	1444258
85280345	85280368	12	+	26627737	HeLa	viability after 12 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.6055055510499076	[485]	[143,125,126]	-5	hSpCas9	negative selection	1526568
85280469	85280492	12	-	26627737	HeLa	viability after 12 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.45102703838195635	[490]	[187,114,146]	-4	hSpCas9	negative selection	1526569
85283650	85283673	12	+	26627737	HeLa	viability after 12 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.6457282859250788	[790]	[778,464,302]	5	hSpCas9	negative selection	1526570
85283866	85283889	12	-	26627737	HeLa	viability after 12 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.3463736295688166	[456]	[354,170,204]	3	hSpCas9	negative selection	1526571
85301263	85301286	12	+	26627737	HeLa	viability after 12 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.15874375129899287	[965]	[237,313,521]	-1	hSpCas9	negative selection	1526572
85301264	85301287	12	+	26627737	HeLa	viability after 12 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.21908444815430395	[883]	[351,206,394]	-2	hSpCas9	negative selection	1526573
85280345	85280368	12	+	26627737	HeLa	viability after 15 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.2275102875968976	[485]	[83,134,298]	-2	hSpCas9	negative selection	1608883
85280469	85280492	12	-	26627737	HeLa	viability after 15 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.47765233075268054	[490]	[146,98,187]	-4	hSpCas9	negative selection	1608884
85283650	85283673	12	+	26627737	HeLa	viability after 15 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.25748598296445446	[790]	[408,414,325]	2	hSpCas9	negative selection	1608885
85283866	85283889	12	-	26627737	HeLa	viability after 15 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.3946642504249	[456]	[378,168,178]	3	hSpCas9	negative selection	1608886
85301263	85301286	12	+	26627737	HeLa	viability after 15 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.2683012978146988	[965]	[302,372,301]	-2	hSpCas9	negative selection	1608887
85301264	85301287	12	+	26627737	HeLa	viability after 15 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.4798860195461937	[883]	[277,332,154]	-4	hSpCas9	negative selection	1608888
85280345	85280368	12	+	26627737	HeLa	viability after 18 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.24689918610808462	[485]	[50,112,298]	-2	hSpCas9	negative selection	1691198
85280469	85280492	12	-	26627737	HeLa	viability after 18 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.45210714898675136	[490]	[101,83,187]	-4	hSpCas9	negative selection	1691199
85283650	85283673	12	+	26627737	HeLa	viability after 18 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.2440708141214678	[790]	[267,342,325]	2	hSpCas9	negative selection	1691200
85283866	85283889	12	-	26627737	HeLa	viability after 18 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.4173919893352089	[456]	[264,135,178]	3	hSpCas9	negative selection	1691201
85301263	85301286	12	+	26627737	HeLa	viability after 18 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.22310150723839262	[965]	[216,316,301]	-2	hSpCas9	negative selection	1691202
85301264	85301287	12	+	26627737	HeLa	viability after 18 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.4952441527726237	[883]	[180,275,154]	-4	hSpCas9	negative selection	1691203
85280345	85280368	12	+	26627737	HCT116	viability after 6 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.7439941041917773	[874]	[85,79]	-4	hSpCas9	negative selection	1773513
85280469	85280492	12	-	26627737	HCT116	viability after 6 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	1.2988112914846293	[1016]	[451,360]	7	hSpCas9	negative selection	1773514
85283650	85283673	12	+	26627737	HCT116	viability after 6 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.11180495884228403	[1086]	[397,66]	0	hSpCas9	negative selection	1773515
85283866	85283889	12	-	26627737	HCT116	viability after 6 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	1.113842311227017	[661]	[163,258]	6	hSpCas9	negative selection	1773516
85301263	85301286	12	+	26627737	HCT116	viability after 6 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.5644186736170589	[1665]	[189,169]	-3	hSpCas9	negative selection	1773517
85301264	85301287	12	+	26627737	HCT116	viability after 6 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.1509193281702892	[1371]	[201,189]	-1	hSpCas9	negative selection	1773518
85280345	85280368	12	+	26627737	HCT116	viability after 8 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.2090777490301559	[420]	[216,155,228]	-2	hSpCas9	negative selection	1855828
85280469	85280492	12	-	26627737	HCT116	viability after 8 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.12829201781056399	[458]	[308,251,268]	1	hSpCas9	negative selection	1855829
85283650	85283673	12	+	26627737	HCT116	viability after 8 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.48093094160542105	[645]	[498,623,368]	5	hSpCas9	negative selection	1855830
85283866	85283889	12	-	26627737	HCT116	viability after 8 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.7906160444017171	[288]	[317,302,206]	7	hSpCas9	negative selection	1855831
85301263	85301286	12	+	26627737	HCT116	viability after 8 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.30207823935698697	[1155]	[467,557,522]	-4	hSpCas9	negative selection	1855832
85301264	85301287	12	+	26627737	HCT116	viability after 8 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.17298113416402805	[579]	[237,357,253]	-2	hSpCas9	negative selection	1855833
85280345	85280368	12	+	26627737	HCT116	viability after 9 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-1.1441579850449637	[874]	[117,95]	-7	hSpCas9	negative selection	1938143
85280469	85280492	12	-	26627737	HCT116	viability after 9 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	1.0835015038256155	[1016]	[899,318]	7	hSpCas9	negative selection	1938144
85283650	85283673	12	+	26627737	HCT116	viability after 9 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.27580917254761206	[1086]	[566,180]	2	hSpCas9	negative selection	1938145
85283866	85283889	12	-	26627737	HCT116	viability after 9 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.46908239732823914	[661]	[261,230]	3	hSpCas9	negative selection	1938146
85301263	85301286	12	+	26627737	HCT116	viability after 9 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.46086317607662597	[1665]	[518,685]	3	hSpCas9	negative selection	1938147
85301264	85301287	12	+	26627737	HCT116	viability after 9 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.36408217581100755	[1371]	[206,345]	-3	hSpCas9	negative selection	1938148
85280345	85280368	12	+	26627737	HCT116	viability after 12 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.6389523351217024	[420]	[81,161,187]	-6	hSpCas9	negative selection	2020458
85280469	85280492	12	-	26627737	HCT116	viability after 12 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.033480015224228166	[458]	[281,223,230]	0	hSpCas9	negative selection	2020459
85283650	85283673	12	+	26627737	HCT116	viability after 12 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.3069312277850884	[645]	[360,456,447]	3	hSpCas9	negative selection	2020460
85283866	85283889	12	-	26627737	HCT116	viability after 12 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.9425855564562825	[288]	[356,250,260]	7	hSpCas9	negative selection	2020461
85301263	85301286	12	+	26627737	HCT116	viability after 12 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.5178106697967679	[1155]	[350,409,517]	-5	hSpCas9	negative selection	2020462
85301264	85301287	12	+	26627737	HCT116	viability after 12 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.003936265615274048	[579]	[153,295,479]	0	hSpCas9	negative selection	2020463
85280345	85280368	12	+	26627737	HCT116	viability after 15 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.312701303817379	[420]	[205,236,142]	-3	hSpCas9	negative selection	2102773
85280469	85280492	12	-	26627737	HCT116	viability after 15 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.3370615604468491	[458]	[443,281,286]	3	hSpCas9	negative selection	2102774
85283650	85283673	12	+	26627737	HCT116	viability after 15 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.20392764219156123	[645]	[493,361,433]	2	hSpCas9	negative selection	2102775
85283866	85283889	12	-	26627737	HCT116	viability after 15 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.4667072739594872	[288]	[290,208,195]	4	hSpCas9	negative selection	2102776
85301263	85301286	12	+	26627737	HCT116	viability after 15 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.6046140568278635	[1155]	[467,439,405]	-6	hSpCas9	negative selection	2102777
85301264	85301287	12	+	26627737	HCT116	viability after 15 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.24280214236823205	[579]	[211,339,283]	-3	hSpCas9	negative selection	2102778
85280345	85280368	12	+	26627737	HCT116	viability after 18 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.5541366463632077	[420]	[66,123,229]	-5	hSpCas9	negative selection	2185088
85280469	85280492	12	-	26627737	HCT116	viability after 18 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.11612596175477763	[458]	[283,149,295]	1	hSpCas9	negative selection	2185089
85283650	85283673	12	+	26627737	HCT116	viability after 18 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.20317622906312316	[645]	[347,420,302]	1	hSpCas9	negative selection	2185090
85283866	85283889	12	-	26627737	HCT116	viability after 18 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.9563756614333837	[288]	[363,252,193]	7	hSpCas9	negative selection	2185091
85301263	85301286	12	+	26627737	HCT116	viability after 18 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.061511186722540856	[1155]	[585,485,537]	0	hSpCas9	negative selection	2185092
85301264	85301287	12	+	26627737	HCT116	viability after 18 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.371132593131136	[579]	[194,134,330]	-3	hSpCas9	negative selection	2185093
85280287	85280310	12	-	24336569	HL60	viability	TTTACTCGGAGGGCTCTTGAGGG	ALX1	ENSG00000180318	0.7041594392150095	[343]	[1185]	6	hSpCas9	negative selection	2247579
85280324	85280347	12	+	24336569	HL60	viability	CATGGGCGCAGGAGGTCCTCTGG	ALX1	ENSG00000180318	0.43773062994123935	[560]	[1607]	4	hSpCas9	negative selection	2247580
85280336	85280359	12	+	24336569	HL60	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.9068792641210193	[465]	[1848]	7	hSpCas9	negative selection	2247581
85280345	85280368	12	+	24336569	HL60	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.5554368758774861	[681]	[2120]	5	hSpCas9	negative selection	2247582
85280393	85280416	12	+	24336569	HL60	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	-0.46293263801337625	[1684]	[2586]	-4	hSpCas9	negative selection	2247583
85280400	85280423	12	+	24336569	HL60	viability	GGCAAATGCGTGCAGGCCTTCGG	ALX1	ENSG00000180318	0.4856224370653326	[784]	[2325]	4	hSpCas9	negative selection	2247584
85280431	85280454	12	-	24336569	HL60	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	-0.3803437054094123	[814]	[1324]	-3	hSpCas9	negative selection	2247585
85280437	85280460	12	-	24336569	HL60	viability	CTCCAAGCGCACGTGATGCTCGG	ALX1	ENSG00000180318	-0.5459549979981738	[731]	[1060]	-5	hSpCas9	negative selection	2247586
85280456	85280479	12	+	24336569	HL60	viability	GGAGAGGACCTCGCCCTGTCAGG	ALX1	ENSG00000180318	0.07931797526635137	[597]	[1336]	0	hSpCas9	negative selection	2247587
85280464	85280487	12	-	24336569	HL60	viability	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	0.3199545879191481	[917]	[2424]	3	hSpCas9	negative selection	2247588
85280287	85280310	12	-	24336569	KBM7	viability	TTTACTCGGAGGGCTCTTGAGGG	ALX1	ENSG00000180318	0.04126818302655266	[330]	[928]	0	hSpCas9	negative selection	2314756
85280324	85280347	12	+	24336569	KBM7	viability	CATGGGCGCAGGAGGTCCTCTGG	ALX1	ENSG00000180318	-0.7750517349496049	[553]	[882]	-5	hSpCas9	negative selection	2314757
85280336	85280359	12	+	24336569	KBM7	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.20467472440802364	[369]	[1162]	2	hSpCas9	negative selection	2314758
85280345	85280368	12	+	24336569	KBM7	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.41936452179852146	[331]	[1210]	4	hSpCas9	negative selection	2314759
85280393	85280416	12	+	24336569	KBM7	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.053011686993198914	[844]	[2390]	0	hSpCas9	negative selection	2314760
85280400	85280423	12	+	24336569	KBM7	viability	GGCAAATGCGTGCAGGCCTTCGG	ALX1	ENSG00000180318	-0.11798849564684621	[675]	[1698]	-1	hSpCas9	negative selection	2314761
85280431	85280454	12	-	24336569	KBM7	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	-0.12598317555892113	[868]	[2171]	-1	hSpCas9	negative selection	2314762
85280437	85280460	12	-	24336569	KBM7	viability	CTCCAAGCGCACGTGATGCTCGG	ALX1	ENSG00000180318	0.23114125658137025	[555]	[1779]	2	hSpCas9	negative selection	2314763
85280456	85280479	12	+	24336569	KBM7	viability	GGAGAGGACCTCGCCCTGTCAGG	ALX1	ENSG00000180318	0.7113243988021777	[364]	[1629]	6	hSpCas9	negative selection	2314764
85280464	85280487	12	-	24336569	KBM7	viability	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	0.5062427605839207	[745]	[2889]	4	hSpCas9	negative selection	2314765
85280100	85280123	12	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCGGGACGTATTAAAGGGACCGG	ALX1	ENSG00000180318	0.34086499588466423	[127,1]	[28,4]	1	dCas9-VP64	positive selection	2440916
85280142	85280165	12	-	25494202	A375	resistance to PLX-4720 (puromycin)	GGAAGGGAAAGCGAGCGCTGCGG	ALX1	ENSG00000180318	5.772673377911175	[20,46]	[6,852]	9	dCas9-VP64	positive selection	2440917
85280100	85280123	12	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCGGGACGTATTAAAGGGACCGG	ALX1	ENSG00000180318	0.033546688033370124	[16,210]	[7,64]	0	dCas9-VP64	positive selection	2536171
85280142	85280165	12	-	25494202	A375	resistance to PLX-4720 (zeocin)	GGAAGGGAAAGCGAGCGCTGCGG	ALX1	ENSG00000180318	-0.06135110560977586	[105,52]	[14,33]	0	dCas9-VP64	positive selection	2536172
85283604	85283627	12	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	0.4160552291448918	[4,5]	[4,6]	8	hSpCas9	positive selection	2970033
85280464	85280487	12	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	-0.13552064172061773	[3,3]	[3,1]	-2	hSpCas9	positive selection	2970034
85283726	85283749	12	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.23431642100686545	[2,3]	[2,3]	5	hSpCas9	positive selection	2970035
85280467	85280490	12	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.9139456567477436	[2,2]	[1,0]	-9	hSpCas9	positive selection	2970036
85283604	85283627	12	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	-0.11671540920894907	[4,4]	[4,1]	-2	hSpCas9	positive selection	3037891
85280464	85280487	12	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	-0.21872020182230784	[3,3]	[3,0]	-3	hSpCas9	positive selection	3037892
85283726	85283749	12	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.18500966748765416	[2,2]	[2,2]	3	hSpCas9	positive selection	3037893
85280467	85280490	12	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.4613302241300713	[2,2]	[2,0]	-6	hSpCas9	positive selection	3037894
85280336	85280359		+	24336571	A375	resistance to PLX after 7 days	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.36547957874037273	[7,5]	[4,5]	-8	hSpCas9	positive selection	3115316
85280435	85280458		+	24336571	A375	resistance to PLX after 7 days	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	-0.19042577949801956	[14,10]	[9,12]	-6	hSpCas9	positive selection	3115326
85283649	85283672		+	24336571	A375	resistance to PLX after 7 days	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.18466615169945405	[11,11]	[10,9]	-6	hSpCas9	positive selection	3115338
85283614	85283637		-	24336571	A375	resistance to PLX after 7 days	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.10180653246996088	[12,12]	[12,10]	-3	hSpCas9	positive selection	3115349
85280336	85280359		+	24336571	A375	resistance to PLX after 14 days	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.06774664621409188	[6,3]	[1,2]	0	hSpCas9	positive selection	3173109
85280435	85280458		+	24336571	A375	resistance to PLX after 14 days	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.3185740543464456	[16,16]	[10,8]	3	hSpCas9	positive selection	3173119
85283649	85283672		+	24336571	A375	resistance to PLX after 14 days	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.6918795580916723	[9,11]	[1,2]	-7	hSpCas9	positive selection	3173131
85283614	85283637		-	24336571	A375	resistance to PLX after 14 days	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.32167496701601594	[10,11]	[4,7]	3	hSpCas9	positive selection	3173142
85280336	85280359		+	24336571	A375	viability after 7 days	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.012309547963720524	[6]	[7,5]	0	hSpCas9	negative selection	3230902
85280435	85280458		+	24336571	A375	viability after 7 days	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5691235427394761	[8]	[14,10]	8	hSpCas9	negative selection	3230912
85283649	85283672		+	24336571	A375	viability after 7 days	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.33840997555019636	[15]	[11,11]	-5	hSpCas9	negative selection	3230924
85283614	85283637		-	24336571	A375	viability after 7 days	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.5026974965732962	[8]	[12,12]	7	hSpCas9	negative selection	3230935
85280336	85280359		+	24336571	A375	viability after 14 days	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.2890154367200203	[6]	[6,3]	-3	hSpCas9	negative selection	3288695
85280435	85280458		+	24336571	A375	viability after 14 days	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.9671117093399838	[8]	[16,16]	9	hSpCas9	negative selection	3288705
85283649	85283672		+	24336571	A375	viability after 14 days	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.4738019816398894	[15]	[9,11]	-5	hSpCas9	negative selection	3288717
85283614	85283637		-	24336571	A375	viability after 14 days	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.41209845204853435	[8]	[10,11]	5	hSpCas9	negative selection	3288728
85280435	85280458	12	+	27383988	293T	resistance to West Nile virus (flavivirus)	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5877695113397602	[70,132]	[0,0]	3	hSpCas9	positive selection	3336386
85301411	85301434	12	-	27383988	293T	resistance to West Nile virus (flavivirus)	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	-0.7625733459918697	[267,250]	[0,0]	-5	hSpCas9	positive selection	3336387
85280336	85280359	12	+	27383988	293T	resistance to West Nile virus (flavivirus)	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	1.0854380922140343	[86,56]	[0,0]	5	hSpCas9	positive selection	3336388
85283649	85283672	12	+	27383988	293T	resistance to West Nile virus (flavivirus)	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.5305763479455964	[220,220]	[0,0]	-4	hSpCas9	positive selection	3355642
85301245	85301268	12	-	27383988	293T	resistance to West Nile virus (flavivirus)	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	4.414051715133287	[121,121]	[16,16]	9	hSpCas9	positive selection	3355643
85283614	85283637	12	-	27383988	293T	resistance to West Nile virus (flavivirus)	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.24051792375928105	[258,258]	[1,1]	1	hSpCas9	positive selection	3355644
85283604	85283627	12	-	26780180	HT29	viability (Avana library 4 designs)	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	0.660183244512669	[]	[]	2	hSpCas9	negative selection	3406117
85280464	85280487	12	-	26780180	HT29	viability (Avana library 4 designs)	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	1.60501836254122	[]	[]	6	hSpCas9	negative selection	3406118
85283725	85283748	12	+	26780180	HT29	viability (Avana library 4 designs)	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.770505675729652	[]	[]	3	hSpCas9	negative selection	3406119
85280466	85280489	12	+	26780180	HT29	viability (Avana library 4 designs)	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	1.35528628749136	[]	[]	5	hSpCas9	negative selection	3406120
85280464	85280487	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	5.51148573622605	[]	[]	8	hSpCas9	negative selection	3478467
85283725	85283748	12	+	26780180	A375	viability (Avana lentiCRISPRv2)	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.779775412170174	[]	[]	-1	hSpCas9	negative selection	3478468
85280466	85280489	12	+	26780180	A375	viability (Avana lentiCRISPRv2)	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-1.55304599431455	[]	[]	-2	hSpCas9	negative selection	3478469
85283604	85283627	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	1.35554933402385	[]	[]	2	hSpCas9	negative selection	3478470
85280432	85280455	12	-	26780180	A375	viability (Avana lentiCRISPRv2)	AGCGCACGTGATGCTCGGCGCGG	ALX1	ENSG00000180318	2.40750253589515	[]	[]	3	hSpCas9	negative selection	3478471
85283612	85283635	12	+	26780180	A375	viability (Avana lentiCRISPRv2)	CACCGAACTGAATAGAGCTATGG	ALX1	ENSG00000180318	1.55216402804858	[]	[]	2	hSpCas9	negative selection	3478472
85280464	85280487	12	-	26780180	A375	viability (Avana lentiGuide)	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	0.497707669656239	[]	[]	0	hSpCas9	negative selection	3587126
85283725	85283748	12	+	26780180	A375	viability (Avana lentiGuide)	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	4.34906660602437	[]	[]	4	hSpCas9	negative selection	3587127
85280466	85280489	12	+	26780180	A375	viability (Avana lentiGuide)	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	3.58400802738417	[]	[]	3	hSpCas9	negative selection	3587128
85283604	85283627	12	-	26780180	A375	viability (Avana lentiGuide)	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	0.235784043961283	[]	[]	0	hSpCas9	negative selection	3587129
85280432	85280455	12	-	26780180	A375	viability (Avana lentiGuide)	AGCGCACGTGATGCTCGGCGCGG	ALX1	ENSG00000180318	0.750838608375059	[]	[]	0	hSpCas9	negative selection	3587130
85283612	85283635	12	+	26780180	A375	viability (Avana lentiGuide)	CACCGAACTGAATAGAGCTATGG	ALX1	ENSG00000180318	-2.84341942291758	[]	[]	-5	hSpCas9	negative selection	3587131
85280464	85280487	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	0.3460639300600395	[3]	[5,5]	9	hSpCas9	positive selection	3695785
85283725	85283748	12	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.8824141724109065	[4]	[3,0]	-8	hSpCas9	positive selection	3695786
85280466	85280489	12	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.21750231931572778	[3]	[2,2]	-3	hSpCas9	positive selection	3695787
85283604	85283627	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	0.08092802632805923	[6]	[6,6]	2	hSpCas9	positive selection	3695788
85280432	85280455	12	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGCGCACGTGATGCTCGGCGCGG	ALX1	ENSG00000180318	0.25590108891425944	[3]	[5,3]	7	hSpCas9	positive selection	3695789
85283612	85283635	12	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CACCGAACTGAATAGAGCTATGG	ALX1	ENSG00000180318	0.16052737706253542	[5]	[5,5]	4	hSpCas9	positive selection	3695790
85280464	85280487	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCTGCTGTCCTGACAGGGCGAGG	ALX1	ENSG00000180318	-0.33183739927728817	[5]	[3,3]	-4	hSpCas9	positive selection	3804392
85283725	85283748	12	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.46111705786332724	[4]	[4,0]	-5	hSpCas9	positive selection	3804393
85280466	85280489	12	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.3185502316920378	[4]	[0,4]	-4	hSpCas9	positive selection	3804394
85283604	85283627	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CTATTCAGTTCGGTGTGAAGGGG	ALX1	ENSG00000180318	0.23540993282786904	[6]	[6,6]	6	hSpCas9	positive selection	3804395
85280432	85280455	12	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGCGCACGTGATGCTCGGCGCGG	ALX1	ENSG00000180318	0.18545132087815963	[4]	[5,3]	4	hSpCas9	positive selection	3804396
85283612	85283635	12	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CACCGAACTGAATAGAGCTATGG	ALX1	ENSG00000180318	0.30110098219305703	[5]	[5,5]	8	hSpCas9	positive selection	3804397
85280336	85280359	12	+	26780180	A375	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.974509516737999	[]	[]	4	hSpCas9	negative selection	3912803
85301411	85301434	12	-	26780180	A375	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.888623930380906	[]	[]	4	hSpCas9	negative selection	3912804
85280435	85280458	12	+	26780180	A375	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.833861757101413	[]	[]	3	hSpCas9	negative selection	3912805
85283649	85283672	12	+	26780180	A375	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.66085645434392	[]	[]	8	hSpCas9	negative selection	3912806
85283614	85283637	12	-	26780180	A375	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	1.10395045289811	[]	[]	5	hSpCas9	negative selection	3912807
85301245	85301268	12	-	26780180	A375	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	1.31852037516722	[]	[]	7	hSpCas9	negative selection	3912808
85280336	85280359	12	+	26780180	HT29	viability (GeCKOv2 library)	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	1.0921409825474	[]	[]	5	hSpCas9	negative selection	4020769
85301411	85301434	12	-	26780180	HT29	viability (GeCKOv2 library)	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.553858672614464	[]	[]	1	hSpCas9	negative selection	4020770
85280435	85280458	12	+	26780180	HT29	viability (GeCKOv2 library)	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.935299072165762	[]	[]	4	hSpCas9	negative selection	4020771
85283649	85283672	12	+	26780180	HT29	viability (GeCKOv2 library)	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.11795584329186	[]	[]	5	hSpCas9	negative selection	4020772
85283614	85283637	12	-	26780180	HT29	viability (GeCKOv2 library)	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	1.61049646608945	[]	[]	8	hSpCas9	negative selection	4020773
85301245	85301268	12	-	26780180	HT29	viability (GeCKOv2 library)	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	1.277491775027	[]	[]	6	hSpCas9	negative selection	4020774
85280336	85280359	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.5287410276300569	[3]	[1,0]	-8	hSpCas9	positive selection	4138406
85280435	85280458	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.2835168884828616	[3]	[1,3]	7	hSpCas9	positive selection	4138417
85283649	85283672	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.3576433278594176	[3]	[2,1]	-7	hSpCas9	positive selection	4138429
85283614	85283637	12	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.09928286468186465	[3]	[1,2]	-2	hSpCas9	positive selection	4138440
85280336	85280359	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.14825226319867374	[3]	[1,3,1,0]	-2	hSpCas9	positive selection	4202428
85280435	85280458	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5627100686056004	[2]	[3,1,1,0]	4	hSpCas9	positive selection	4202439
85283649	85283672	12	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.048800983489987515	[3]	[2,3,1,0]	0	hSpCas9	positive selection	4202451
85283614	85283637	12	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.9826097882973535	[2]	[0,4,1,2]	7	hSpCas9	positive selection	4202462
85280435	85280458	12	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.286329794556527	[2]	[2,2]	6	hSpCas9	positive selection	4255436
85301411	85301434	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.4293562208180395	[4]	[3,4]	8	hSpCas9	positive selection	4255437
85280336	85280359	12	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.26983514078181586	[1]	[1,0]	-5	hSpCas9	positive selection	4255438
85283649	85283672	12	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.35503203323839433	[3]	[2,0]	-6	hSpCas9	positive selection	4319718
85301245	85301268	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.31241045825137403	[2]	[3,2]	6	hSpCas9	positive selection	4319719
85283614	85283637	12	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.461195195028299	[3]	[3,3]	8	hSpCas9	positive selection	4319720
85280441	85280463	12	+	27760321	OCIAML3	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.4216910093686268	[771,648]	[602,921]	5	hSpCas9	negative selection	4377577
85283560	85283582	12	+	27760321	OCIAML3	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.41049585459899285	[363,269]	[234,445]	5	hSpCas9	negative selection	4377578
85283578	85283600	12	+	27760321	OCIAML3	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-1.7185856910747193	[43,30]	[0,18]	-8	hSpCas9	negative selection	4377579
85283605	85283627	12	-	27760321	OCIAML3	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.5263713213781362	[334,307]	[309,429]	6	hSpCas9	negative selection	4377580
85283650	85283672	12	+	27760321	OCIAML3	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.24613699968493846	[409,297]	[243,430]	3	hSpCas9	negative selection	4377581
85280441	85280463	12	+	27760321	OCIAML2	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	-0.026215413588636105	[771,648]	[544,584]	0	hSpCas9	negative selection	4461040
85283560	85283582	12	+	27760321	OCIAML2	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.838589310418961	[363,269]	[378,553]	9	hSpCas9	negative selection	4461041
85283578	85283600	12	+	27760321	OCIAML2	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-0.4346537781460329	[43,30]	[13,32]	-5	hSpCas9	negative selection	4461042
85283605	85283627	12	-	27760321	OCIAML2	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.5478688096254825	[334,307]	[377,382]	7	hSpCas9	negative selection	4461043
85283650	85283672	12	+	27760321	OCIAML2	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5178412805476068	[409,297]	[314,525]	7	hSpCas9	negative selection	4461044
85280441	85280463	12	+	27760321	MV411	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	1.3064680512692817	[771,648]	[1665,387]	8	hSpCas9	negative selection	4544503
85283560	85283582	12	+	27760321	MV411	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	-1.16010091059115	[363,269]	[63,135]	-6	hSpCas9	negative selection	4544504
85283578	85283600	12	+	27760321	MV411	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-0.8469902574112211	[43,30]	[21,0]	-5	hSpCas9	negative selection	4544505
85283605	85283627	12	-	27760321	MV411	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	1.1685071564640737	[334,307]	[487,459]	8	hSpCas9	negative selection	4544506
85283650	85283672	12	+	27760321	MV411	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5277774280883979	[409,297]	[239,473]	4	hSpCas9	negative selection	4544507
85280441	85280463	12	+	27760321	HL60	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.3981694902171695	[771,648]	[676,851]	5	hSpCas9	negative selection	4627966
85283560	85283582	12	+	27760321	HL60	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.1919692079782495	[363,269]	[414,163]	2	hSpCas9	negative selection	4627967
85283578	85283600	12	+	27760321	HL60	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	1.745480147455947	[43,30]	[87,115]	9	hSpCas9	negative selection	4627968
85283605	85283627	12	-	27760321	HL60	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.2404856148539499	[334,307]	[250,372]	3	hSpCas9	negative selection	4627969
85283650	85283672	12	+	27760321	HL60	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.2264739422958455	[409,297]	[440,912]	9	hSpCas9	negative selection	4627970
85280441	85280463	12	+	27760321	HT1080	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.3536414767210264	[648,506]	[372,207]	1	hSpCas9	negative selection	4711429
85283560	85283582	12	+	27760321	HT1080	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	2.089875574559851	[269,210]	[605,190]	9	hSpCas9	negative selection	4711430
85283578	85283600	12	+	27760321	HT1080	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-0.7025970475661927	[30,23]	[0,12]	-3	hSpCas9	negative selection	4711431
85283605	85283627	12	-	27760321	HT1080	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.8496663151295523	[307,240]	[177,217]	4	hSpCas9	negative selection	4711432
85283650	85283672	12	+	27760321	HT1080	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	2.0413217119830063	[297,232]	[559,298]	9	hSpCas9	negative selection	4711433
85280441	85280463	12	+	27760321	HT29	viability after 7 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	-0.19525808237178865	[648,506]	[572,600,757]	-3	hSpCas9	negative selection	4794892
85283560	85283582	12	+	27760321	HT29	viability after 7 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	1.029767948094654	[269,210]	[562,642,675]	9	hSpCas9	negative selection	4794893
85283578	85283600	12	+	27760321	HT29	viability after 7 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	0.05158539619881841	[30,23]	[18,9,76]	0	hSpCas9	negative selection	4794894
85283605	85283627	12	-	27760321	HT29	viability after 7 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.03107847311072509	[307,240]	[424,283,380]	0	hSpCas9	negative selection	4794895
85283650	85283672	12	+	27760321	HT29	viability after 7 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.007489083522608	[297,232]	[896,724,472]	9	hSpCas9	negative selection	4794896
85280441	85280463	12	+	27760321	HT29	viability after 25 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.14147243704633583	[648,506]	[804,663,708]	1	hSpCas9	negative selection	4878355
85283560	85283582	12	+	27760321	HT29	viability after 25 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.5490324989441369	[269,210]	[196,505,528]	6	hSpCas9	negative selection	4878356
85283578	85283600	12	+	27760321	HT29	viability after 25 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	0.6232030386658538	[30,23]	[49,54,38]	7	hSpCas9	negative selection	4878357
85283605	85283627	12	-	27760321	HT29	viability after 25 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.09335431536466487	[307,240]	[286,252,481]	0	hSpCas9	negative selection	4878358
85283650	85283672	12	+	27760321	HT29	viability after 25 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.457127447392459	[297,232]	[580,376,264]	5	hSpCas9	negative selection	4878359
85280441	85280463	12	+	27760321	HT29	viability after 22 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.16991832833629938	[648,506]	[516,950,793]	1	hSpCas9	negative selection	4961818
85283560	85283582	12	+	27760321	HT29	viability after 22 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.41249910560980096	[269,210]	[400,275,432]	4	hSpCas9	negative selection	4961819
85283578	85283600	12	+	27760321	HT29	viability after 22 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	0.7015608608246637	[30,23]	[28,36,89]	7	hSpCas9	negative selection	4961820
85283605	85283627	12	-	27760321	HT29	viability after 22 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.3672310397048167	[307,240]	[463,318,443]	4	hSpCas9	negative selection	4961821
85283650	85283672	12	+	27760321	HT29	viability after 22 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.6976341209985013	[297,232]	[688,234,564]	7	hSpCas9	negative selection	4961822
85280441	85280463	12	+	27760321	HT29	viability after 19 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.2217543646171004	[648,506]	[569,488,1214]	2	hSpCas9	negative selection	5045281
85283560	85283582	12	+	27760321	HT29	viability after 19 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.47099834855329686	[269,210]	[401,315,425]	4	hSpCas9	negative selection	5045282
85283578	85283600	12	+	27760321	HT29	viability after 19 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	1.6152010490618935	[30,23]	[98,9,176]	9	hSpCas9	negative selection	5045283
85283605	85283627	12	-	27760321	HT29	viability after 19 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	1.0816952294185225	[307,240]	[438,339,1170]	9	hSpCas9	negative selection	5045284
85283650	85283672	12	+	27760321	HT29	viability after 19 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.43848391157175864	[297,232]	[522,337,385]	4	hSpCas9	negative selection	5045285
85280441	85280463	12	+	27760321	HT29	viability after 16 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.27943668313310155	[648,506]	[592,880,859]	3	hSpCas9	negative selection	5128744
85283560	85283582	12	+	27760321	HT29	viability after 16 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.6829113935450206	[269,210]	[356,578,348]	7	hSpCas9	negative selection	5128745
85283578	85283600	12	+	27760321	HT29	viability after 16 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-1.4471169568680962	[30,23]	[22,8,2]	-8	hSpCas9	negative selection	5128746
85283605	85283627	12	-	27760321	HT29	viability after 16 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.11509812991891205	[307,240]	[431,383,189]	1	hSpCas9	negative selection	5128747
85283650	85283672	12	+	27760321	HT29	viability after 16 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.7420130383866417	[297,232]	[521,453,517]	8	hSpCas9	negative selection	5128748
85280441	85280463	12	+	27760321	HT29	viability after 13 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.32537913550258935	[648,506]	[1066,748,711]	4	hSpCas9	negative selection	5212207
85283560	85283582	12	+	27760321	HT29	viability after 13 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	1.0282984908194968	[269,210]	[532,616,557]	9	hSpCas9	negative selection	5212208
85283578	85283600	12	+	27760321	HT29	viability after 13 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	-0.15603263426760305	[30,23]	[34,7,41]	-2	hSpCas9	negative selection	5212209
85283605	85283627	12	-	27760321	HT29	viability after 13 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.13772755513359003	[307,240]	[443,342,268]	1	hSpCas9	negative selection	5212210
85283650	85283672	12	+	27760321	HT29	viability after 13 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.793615525330561	[297,232]	[455,441,693]	9	hSpCas9	negative selection	5212211
85280441	85280463	12	+	27760321	HT29	viability after 10 days	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.1459194779203351	[648,506]	[803,1087,517]	2	hSpCas9	negative selection	5295670
85283560	85283582	12	+	27760321	HT29	viability after 10 days	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.6753559086514505	[269,210]	[471,569,400]	8	hSpCas9	negative selection	5295671
85283578	85283600	12	+	27760321	HT29	viability after 10 days	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	1.085055103983049	[30,23]	[60,49,105]	9	hSpCas9	negative selection	5295672
85283605	85283627	12	-	27760321	HT29	viability after 10 days	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.07434995644696701	[307,240]	[419,340,321]	1	hSpCas9	negative selection	5295673
85283650	85283672	12	+	27760321	HT29	viability after 10 days	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.8094446442983465	[297,232]	[579,381,770]	9	hSpCas9	negative selection	5295674
85280441	85280463	12	+	27760321	MOLM13	viability	GCATCACGTGCGCTTGGAGAGG	ALX1	ENSG00000180318	0.02843502638467643	[771,648]	[416,398]	0	hSpCas9	negative selection	5379133
85283560	85283582	12	+	27760321	MOLM13	viability	CTCTGGGTACAGTGAACTATGG	ALX1	ENSG00000180318	0.012956740084259954	[363,269]	[247,119]	0	hSpCas9	negative selection	5379134
85283578	85283600	12	+	27760321	MOLM13	viability	ATGGGATCACTAAAGTAGAAGG	ALX1	ENSG00000180318	2.267932266878886	[43,30]	[217,0]	9	hSpCas9	negative selection	5379135
85283605	85283627	12	-	27760321	MOLM13	viability	CTATTCAGTTCGGTGTGAAGGG	ALX1	ENSG00000180318	0.060334700089749393	[334,307]	[106,258]	0	hSpCas9	negative selection	5379136
85283650	85283672	12	+	27760321	MOLM13	viability	TCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.3687558461541962	[409,297]	[775,284]	8	hSpCas9	negative selection	5379137
85280243	85280266	12	+	27661255	K562	viability	GCGACAGTCTTCCAGGATTATGG	ALX1	ENSG00000180318	-0.05611424038505747	[1142,1022]	[924,755]	-2	dCas9-KRAB	negative selection	5461937
85280685	85280708	12	+	27661255	K562	viability	GGAGGGAGAGATCCAGCACCGGG	ALX1	ENSG00000180318	0.025460514337080187	[1296,1483]	[1326,957]	0	dCas9-KRAB	negative selection	5461938
85280711	85280734	12	-	27661255	K562	viability	GCGAAAGGAGAATCGCTCAGAGG	ALX1	ENSG00000180318	0.3682597792957474	[738,1087]	[983,893]	9	dCas9-KRAB	negative selection	5461939
85280435	85280458	12	+	27260156	BXPC3	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5424502695736093	[341]	[442,593,498,550]	9	hSpCas9	negative selection	5527896
85301411	85301434	12	-	27260156	BXPC3	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.2794789699887812	[996]	[1062,1055,1518,1479]	6	hSpCas9	negative selection	5527897
85280336	85280359	12	+	27260156	BXPC3	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.2545753924482266	[183]	[189,232,278,217]	5	hSpCas9	negative selection	5527898
85283649	85283672	12	+	27260156	BXPC3	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.33603378253412647	[459]	[547,471,653,787]	7	hSpCas9	negative selection	5592178
85301245	85301268	12	-	27260156	BXPC3	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.0506146265303497	[437]	[420,525,430,523]	1	hSpCas9	negative selection	5592179
85283614	85283637	12	-	27260156	BXPC3	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.31079460323077807	[506]	[624,623,669,719]	6	hSpCas9	negative selection	5592180
85280435	85280458	12	+	27260156	A673	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.33773314620533235	[341]	[535,543,484,510]	7	hSpCas9	negative selection	5649147
85301411	85301434	12	-	27260156	A673	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.08720129306163726	[996]	[1396,1142,1216,1349]	2	hSpCas9	negative selection	5649148
85280336	85280359	12	+	27260156	A673	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.4444809067301405	[183]	[372,212,258,371]	8	hSpCas9	negative selection	5649149
85283649	85283672	12	+	27260156	A673	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.18224930926314192	[459]	[785,635,462,640]	4	hSpCas9	negative selection	5713429
85301245	85301268	12	-	27260156	A673	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.2103286090870783	[437]	[421,422,439,533]	-5	hSpCas9	negative selection	5713430
85283614	85283637	12	-	27260156	A673	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.15201554885210056	[506]	[706,638,623,742]	3	hSpCas9	negative selection	5713431
85280435	85280458	12	+	27260156	A375	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.3288188616025145	[341]	[506,545,501,460]	5	hSpCas9	negative selection	5770398
85301411	85301434	12	-	27260156	A375	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.42383734683461594	[996]	[1521,1688,1552,1502]	6	hSpCas9	negative selection	5770399
85280336	85280359	12	+	27260156	A375	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.20661875136784613	[183]	[135,437,197,244]	3	hSpCas9	negative selection	5770400
85283649	85283672	12	+	27260156	A375	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.3679106918584234	[459]	[840,800,684,484]	5	hSpCas9	negative selection	5834680
85301245	85301268	12	-	27260156	A375	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.08167002192025327	[437]	[590,475,375,487]	-1	hSpCas9	negative selection	5834681
85283614	85283637	12	-	27260156	A375	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.018754226087252662	[506]	[543,672,584,549]	0	hSpCas9	negative selection	5834682
85280435	85280458	12	+	27260156	COLO741	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.4748669610842222	[341]	[663,675,493]	8	hSpCas9	negative selection	5891649
85301411	85301434	12	-	27260156	COLO741	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.308430125775891	[996]	[1713,1611,1425]	6	hSpCas9	negative selection	5891650
85280336	85280359	12	+	27260156	COLO741	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.42830586192130704	[183]	[482,299,188]	8	hSpCas9	negative selection	5891651
85283649	85283672	12	+	27260156	COLO741	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5118660202416181	[459]	[829,778,897]	8	hSpCas9	negative selection	5955931
85301245	85301268	12	-	27260156	COLO741	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.25101941215397766	[437]	[542,405,466]	-5	hSpCas9	negative selection	5955932
85283614	85283637	12	-	27260156	COLO741	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.031383691183271584	[506]	[726,562,618]	0	hSpCas9	negative selection	5955933
85280435	85280458	12	+	27260156	CAL120	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5729508217709145	[341]	[589,697,604]	8	hSpCas9	negative selection	6012900
85301411	85301434	12	-	27260156	CAL120	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.5957934327545249	[996]	[2193,1813,1667]	8	hSpCas9	negative selection	6012901
85280336	85280359	12	+	27260156	CAL120	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.5927487105735331	[183]	[325,535,173]	8	hSpCas9	negative selection	6012902
85283649	85283672	12	+	27260156	CAL120	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5062755430124152	[459]	[839,908,695]	7	hSpCas9	negative selection	6077182
85301245	85301268	12	-	27260156	CAL120	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.11777814903815112	[437]	[621,414,487]	-2	hSpCas9	negative selection	6077183
85283614	85283637	12	-	27260156	CAL120	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.5869753588225197	[506]	[1323,668,906]	8	hSpCas9	negative selection	6077184
85280435	85280458	12	+	27260156	CADOES1	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.7598981387282202	[341]	[495,482,699,472]	9	hSpCas9	negative selection	6134151
85301411	85301434	12	-	27260156	CADOES1	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.17924659962106593	[996]	[900,1042,978,1285]	3	hSpCas9	negative selection	6134152
85280336	85280359	12	+	27260156	CADOES1	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.1898750595061942	[183]	[105,188,188,300]	3	hSpCas9	negative selection	6134153
85283649	85283672	12	+	27260156	CADOES1	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5507295634988256	[459]	[608,622,642,631]	8	hSpCas9	negative selection	6198433
85301245	85301268	12	-	27260156	CADOES1	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.24746582246637533	[437]	[369,367,357,273]	-4	hSpCas9	negative selection	6198434
85283614	85283637	12	-	27260156	CADOES1	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.30044400342382416	[506]	[440,581,621,681]	5	hSpCas9	negative selection	6198435
85280435	85280458	12	+	27260156	EWS502	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.4440133048441105	[341]	[561,542,421,425]	7	hSpCas9	negative selection	6255402
85301411	85301434	12	-	27260156	EWS502	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	-0.12352859330976262	[996]	[1021,970,955,913]	-2	hSpCas9	negative selection	6255403
85280336	85280359	12	+	27260156	EWS502	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.11646218311596357	[183]	[199,213,246,182]	2	hSpCas9	negative selection	6255404
85283649	85283672	12	+	27260156	EWS502	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.2074579789997179	[459]	[667,453,532,585]	3	hSpCas9	negative selection	6319684
85301245	85301268	12	-	27260156	EWS502	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.4241029898386019	[437]	[319,398,401,255]	-6	hSpCas9	negative selection	6319685
85283614	85283637	12	-	27260156	EWS502	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.3198575392810135	[506]	[401,774,725,814]	5	hSpCas9	negative selection	6319686
85280435	85280458	12	+	27260156	EW8	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.6488781043754748	[341]	[511,472,326,451]	9	hSpCas9	negative selection	6376653
85301411	85301434	12	-	27260156	EW8	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.047126598611448633	[996]	[1007,726,603,1054]	0	hSpCas9	negative selection	6376654
85280336	85280359	12	+	27260156	EW8	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.23488587355794088	[183]	[270,246,101,104]	4	hSpCas9	negative selection	6376655
85283649	85283672	12	+	27260156	EW8	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	-0.005475761190268734	[459]	[292,395,417,360]	0	hSpCas9	negative selection	6440935
85301245	85301268	12	-	27260156	EW8	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.163328704610597	[437]	[341,368,227,345]	-3	hSpCas9	negative selection	6440936
85283614	85283637	12	-	27260156	EW8	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.1793379648538178	[506]	[648,422,417,397]	3	hSpCas9	negative selection	6440937
85280435	85280458	12	+	27260156	CORL105	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5548907829236491	[341]	[480,621,657,597]	8	hSpCas9	negative selection	6497904
85301411	85301434	12	-	27260156	CORL105	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.07273245558355224	[996]	[1211,1198,1511,1031]	1	hSpCas9	negative selection	6497905
85280336	85280359	12	+	27260156	CORL105	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.583400920776666	[183]	[312,349,346,283]	9	hSpCas9	negative selection	6497906
85283649	85283672	12	+	27260156	CORL105	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.16662453160357443	[459]	[749,477,784,440]	3	hSpCas9	negative selection	6562186
85301245	85301268	12	-	27260156	CORL105	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.19230460240624442	[437]	[420,474,495,408]	-4	hSpCas9	negative selection	6562187
85283614	85283637	12	-	27260156	CORL105	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.2681253398050111	[506]	[566,759,730,796]	5	hSpCas9	negative selection	6562188
85280435	85280458	12	+	27260156	HS294T	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5955603772879393	[341]	[328,146,135,170]	7	hSpCas9	negative selection	6619155
85301411	85301434	12	-	27260156	HS294T	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.46682442852837136	[996]	[393,562,620,569]	6	hSpCas9	negative selection	6619156
85280336	85280359	12	+	27260156	HS294T	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.5304374735694841	[183]	[76,128,74,136]	7	hSpCas9	negative selection	6619157
85283649	85283672	12	+	27260156	HS294T	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.1791964702523229	[459]	[220,245,179,144]	2	hSpCas9	negative selection	6683437
85301245	85301268	12	-	27260156	HS294T	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.0432734004933594	[437]	[65,242,168,189]	0	hSpCas9	negative selection	6683438
85283614	85283637	12	-	27260156	HS294T	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.24049888713087697	[506]	[267,166,218,277]	3	hSpCas9	negative selection	6683439
85280435	85280458	12	+	27260156	HCC44	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.19545446832351093	[341]	[552,331,396,501]	3	hSpCas9	negative selection	6740406
85301411	85301434	12	-	27260156	HCC44	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.37393516617223455	[996]	[1465,1299,1802,1349]	6	hSpCas9	negative selection	6740407
85280336	85280359	12	+	27260156	HCC44	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.5981412089282586	[183]	[415,325,296,225]	8	hSpCas9	negative selection	6740408
85283649	85283672	12	+	27260156	HCC44	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5407761757402896	[459]	[808,735,847,654]	8	hSpCas9	negative selection	6804688
85301245	85301268	12	-	27260156	HCC44	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.3268532246114198	[437]	[412,386,496,302]	-5	hSpCas9	negative selection	6804689
85283614	85283637	12	-	27260156	HCC44	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.40303983501717316	[506]	[805,558,1065,683]	6	hSpCas9	negative selection	6804690
85280435	85280458	12	+	27260156	G402	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	1.0630886160943218	[341]	[521,836,633,1411]	9	hSpCas9	negative selection	6861657
85301411	85301434	12	-	27260156	G402	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.21827617773425723	[996]	[1186,1810,1368,1284]	2	hSpCas9	negative selection	6861658
85280336	85280359	12	+	27260156	G402	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.9730908647327079	[183]	[519,561,283,414]	9	hSpCas9	negative selection	6861659
85283649	85283672	12	+	27260156	G402	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.3780511635789254	[459]	[2566,798,838,1732]	9	hSpCas9	negative selection	6925939
85301245	85301268	12	-	27260156	G402	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.10803000985772473	[437]	[847,521,541,432]	1	hSpCas9	negative selection	6925940
85283614	85283637	12	-	27260156	G402	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.34738084421581933	[506]	[976,717,736,738]	4	hSpCas9	negative selection	6925941
85280435	85280458	12	+	27260156	L33	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.30441476175026405	[341]	[500,203,196,374]	6	hSpCas9	negative selection	6982908
85301411	85301434	12	-	27260156	L33	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.14748181138948424	[996]	[1026,438,699,1205]	3	hSpCas9	negative selection	6982909
85280336	85280359	12	+	27260156	L33	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.4827582060271788	[183]	[251,116,125,289]	8	hSpCas9	negative selection	6982910
85283649	85283672	12	+	27260156	L33	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.054162319502081974	[459]	[491,201,262,505]	1	hSpCas9	negative selection	7047190
85301245	85301268	12	-	27260156	L33	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.5572949993134728	[437]	[265,133,212,259]	-8	hSpCas9	negative selection	7047191
85283614	85283637	12	-	27260156	L33	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.09021176146278981	[506]	[509,135,320,550]	-2	hSpCas9	negative selection	7047192
85280435	85280458	12	+	27260156	K562	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.536028102411353	[341]	[537,378,441,407]	6	hSpCas9	negative selection	7104159
85301411	85301434	12	-	27260156	K562	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.500706027364304	[996]	[1433,962,1929,735]	6	hSpCas9	negative selection	7104160
85280336	85280359	12	+	27260156	K562	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.48712235469541587	[183]	[189,400,181,155]	6	hSpCas9	negative selection	7104161
85283649	85283672	12	+	27260156	K562	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.30157350093364266	[459]	[326,439,895,347]	4	hSpCas9	negative selection	7168441
85301245	85301268	12	-	27260156	K562	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.056487427809956775	[437]	[341,466,201,472]	0	hSpCas9	negative selection	7168442
85283614	85283637	12	-	27260156	K562	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.7186058691846431	[506]	[701,662,1041,564]	8	hSpCas9	negative selection	7168443
85280435	85280458	12	+	27260156	HT29	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.4708922305614613	[341]	[573,623,426,667]	8	hSpCas9	negative selection	7225410
85301411	85301434	12	-	27260156	HT29	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.20262594540696832	[996]	[1568,1453,1481,1116]	4	hSpCas9	negative selection	7225411
85280336	85280359	12	+	27260156	HT29	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.5614913310906513	[183]	[387,484,224,247]	9	hSpCas9	negative selection	7225412
85283649	85283672	12	+	27260156	HT29	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.32448347466152644	[459]	[670,775,589,755]	6	hSpCas9	negative selection	7289692
85301245	85301268	12	-	27260156	HT29	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.18624844720179634	[437]	[471,421,486,481]	-4	hSpCas9	negative selection	7289693
85283614	85283637	12	-	27260156	HT29	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.4144201791286282	[506]	[1036,900,740,645]	7	hSpCas9	negative selection	7289694
85280435	85280458	12	+	27260156	MHHES1	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.3452977810053591	[341]	[657,765,327,288]	6	hSpCas9	negative selection	7346661
85301411	85301434	12	-	27260156	MHHES1	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.3904422251021993	[996]	[1683,1545,1443,1261]	7	hSpCas9	negative selection	7346662
85280336	85280359	12	+	27260156	MHHES1	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.6514377970419336	[183]	[423,388,214,291]	9	hSpCas9	negative selection	7346663
85283649	85283672	12	+	27260156	MHHES1	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.3829322216070054	[459]	[798,642,703,575]	7	hSpCas9	negative selection	7410943
85301245	85301268	12	-	27260156	MHHES1	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.3785273903125052	[437]	[379,436,340,360]	-6	hSpCas9	negative selection	7410944
85283614	85283637	12	-	27260156	MHHES1	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.1628360746318644	[506]	[944,750,445,483]	3	hSpCas9	negative selection	7410945
85280435	85280458	12	+	27260156	MEWO	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.35121760849764866	[341]	[421,419,384]	7	hSpCas9	negative selection	7467912
85301411	85301434	12	-	27260156	MEWO	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	-0.008271227121254388	[996]	[1202,814,817]	0	hSpCas9	negative selection	7467913
85280336	85280359	12	+	27260156	MEWO	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.05015942338157808	[183]	[207,131,166]	-1	hSpCas9	negative selection	7467914
85283649	85283672	12	+	27260156	MEWO	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.06807313818459072	[459]	[530,414,422]	1	hSpCas9	negative selection	7532194
85301245	85301268	12	-	27260156	MEWO	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.20149978003632513	[437]	[504,314,277]	-4	hSpCas9	negative selection	7532195
85283614	85283637	12	-	27260156	MEWO	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.16691734906142053	[506]	[730,504,399]	3	hSpCas9	negative selection	7532196
85280435	85280458	12	+	27260156	LNCAPCLONEFGC	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.7167895490113972	[341]	[359,349,894,501]	9	hSpCas9	negative selection	7589163
85301411	85301434	12	-	27260156	LNCAPCLONEFGC	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.45074431118641334	[996]	[1142,1285,1510,1035]	7	hSpCas9	negative selection	7589164
85280336	85280359	12	+	27260156	LNCAPCLONEFGC	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.8242634778370973	[183]	[298,359,207,295]	9	hSpCas9	negative selection	7589165
85283649	85283672	12	+	27260156	LNCAPCLONEFGC	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5171396015804397	[459]	[534,629,751,489]	7	hSpCas9	negative selection	7653445
85301245	85301268	12	-	27260156	LNCAPCLONEFGC	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.10697704369536282	[437]	[412,333,416,317]	-2	hSpCas9	negative selection	7653446
85283614	85283637	12	-	27260156	LNCAPCLONEFGC	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.15974353777458872	[506]	[335,583,616,528]	2	hSpCas9	negative selection	7653447
85280435	85280458	12	+	27260156	PANC0327	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.8644253996121165	[341]	[414,434,551,813]	9	hSpCas9	negative selection	7710414
85301411	85301434	12	-	27260156	PANC0327	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.24296813041014698	[996]	[1104,948,848,1305]	3	hSpCas9	negative selection	7710415
85280336	85280359	12	+	27260156	PANC0327	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	-0.23342273053491813	[183]	[107,174,178,86]	-3	hSpCas9	negative selection	7710416
85283649	85283672	12	+	27260156	PANC0327	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.6540626309895263	[459]	[442,921,433,719]	8	hSpCas9	negative selection	7774696
85301245	85301268	12	-	27260156	PANC0327	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.08533509250011256	[437]	[545,246,368,334]	-1	hSpCas9	negative selection	7774697
85283614	85283637	12	-	27260156	PANC0327	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.5515712328460822	[506]	[1050,614,537,473]	7	hSpCas9	negative selection	7774698
85280435	85280458	12	+	27260156	NCIH2009	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.8114090341022553	[341]	[808,859,838]	9	hSpCas9	negative selection	7831665
85301411	85301434	12	-	27260156	NCIH2009	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.3921712846868912	[996]	[2288,1455,1833]	5	hSpCas9	negative selection	7831666
85280336	85280359	12	+	27260156	NCIH2009	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.3201798242463765	[183]	[485,262,246]	4	hSpCas9	negative selection	7831667
85283649	85283672	12	+	27260156	NCIH2009	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.40661623172480144	[459]	[1107,574,926]	5	hSpCas9	negative selection	7895947
85301245	85301268	12	-	27260156	NCIH2009	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.740301885894499	[437]	[452,392,268]	-7	hSpCas9	negative selection	7895948
85283614	85283637	12	-	27260156	NCIH2009	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.29130109241347957	[506]	[1136,947,565]	4	hSpCas9	negative selection	7895949
85280435	85280458	12	+	27260156	NCIH1373	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.47402420796016875	[341]	[437,616,504,142]	4	hSpCas9	negative selection	7952916
85301411	85301434	12	-	27260156	NCIH1373	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.46201653741286397	[996]	[1664,1322,1383,483]	4	hSpCas9	negative selection	7952917
85280336	85280359	12	+	27260156	NCIH1373	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.671868903425989	[183]	[209,212,358,140]	6	hSpCas9	negative selection	7952918
85283649	85283672	12	+	27260156	NCIH1373	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	1.0195991695028834	[459]	[1220,1024,960,261]	9	hSpCas9	negative selection	8017198
85301245	85301268	12	-	27260156	NCIH1373	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.367342651487477	[437]	[1059,309,664,155]	3	hSpCas9	negative selection	8017199
85283614	85283637	12	-	27260156	NCIH1373	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.5628344189840888	[506]	[893,408,653,392]	5	hSpCas9	negative selection	8017200
85280435	85280458	12	+	27260156	PATU8902	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.8991433408932505	[341]	[946,825,647,1226]	8	hSpCas9	negative selection	8074167
85301411	85301434	12	-	27260156	PATU8902	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.6584888038998911	[996]	[1625,2690,1801,2814]	7	hSpCas9	negative selection	8074168
85280336	85280359	12	+	27260156	PATU8902	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.4413527853729805	[183]	[460,254,254,452]	4	hSpCas9	negative selection	8074169
85283649	85283672	12	+	27260156	PATU8902	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.7232160927419198	[459]	[1205,1074,1008,926]	7	hSpCas9	negative selection	8138449
85301245	85301268	12	-	27260156	PATU8902	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.014269087996416727	[437]	[593,572,547,781]	0	hSpCas9	negative selection	8138450
85283614	85283637	12	-	27260156	PATU8902	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.20499430709928396	[506]	[1107,800,763,553]	2	hSpCas9	negative selection	8138451
85280435	85280458	12	+	27260156	PANC1	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.6886351995996145	[341]	[459,535,464,442]	9	hSpCas9	negative selection	8195418
85301411	85301434	12	-	27260156	PANC1	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.4197069212495299	[996]	[1172,1634,1148,768]	6	hSpCas9	negative selection	8195419
85280336	85280359	12	+	27260156	PANC1	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.6202631932733773	[183]	[179,432,155,238]	8	hSpCas9	negative selection	8195420
85283649	85283672	12	+	27260156	PANC1	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5551509976508314	[459]	[544,747,706,381]	8	hSpCas9	negative selection	8259700
85301245	85301268	12	-	27260156	PANC1	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.10004658617502377	[437]	[231,538,296,367]	-2	hSpCas9	negative selection	8259701
85283614	85283637	12	-	27260156	PANC1	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.5840967832904276	[506]	[723,808,667,461]	8	hSpCas9	negative selection	8259702
85280435	85280458	12	+	27260156	PANC0813	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.38434315069042235	[341]	[602,525,561,418]	6	hSpCas9	negative selection	8316669
85301411	85301434	12	-	27260156	PANC0813	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.2730425685864035	[996]	[1547,1099,1495,1520]	4	hSpCas9	negative selection	8316670
85280336	85280359	12	+	27260156	PANC0813	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.3918727144123936	[183]	[290,332,307,206]	6	hSpCas9	negative selection	8316671
85283649	85283672	12	+	27260156	PANC0813	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.23517666656160133	[459]	[543,710,738,545]	4	hSpCas9	negative selection	8380951
85301245	85301268	12	-	27260156	PANC0813	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.15606060878436875	[437]	[533,521,471,749]	2	hSpCas9	negative selection	8380952
85283614	85283637	12	-	27260156	PANC0813	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	-0.00023459367486845795	[506]	[450,576,655,678]	0	hSpCas9	negative selection	8380953
85280435	85280458	12	+	27260156	RDES	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.4997150296388528	[341]	[502,514,566,651]	7	hSpCas9	negative selection	8437920
85301411	85301434	12	-	27260156	RDES	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.15429732444665567	[996]	[1024,1350,1243,1528]	2	hSpCas9	negative selection	8437921
85280336	85280359	12	+	27260156	RDES	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.3647474212461581	[183]	[163,240,333,390]	6	hSpCas9	negative selection	8437922
85283649	85283672	12	+	27260156	RDES	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.31534181932147937	[459]	[530,565,633,964]	5	hSpCas9	negative selection	8502202
85301245	85301268	12	-	27260156	RDES	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.09082051440991948	[437]	[419,426,595,450]	-1	hSpCas9	negative selection	8502203
85283614	85283637	12	-	27260156	RDES	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.04149871939162089	[506]	[488,700,601,603]	0	hSpCas9	negative selection	8502204
85280435	85280458	12	+	27260156	PC3	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.14658933957840414	[341]	[172,461,720,341]	3	hSpCas9	negative selection	8559171
85301411	85301434	12	-	27260156	PC3	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.17762489349191735	[996]	[697,1016,1875,1289]	4	hSpCas9	negative selection	8559172
85280336	85280359	12	+	27260156	PC3	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.3296330926230613	[183]	[177,87,513,258]	7	hSpCas9	negative selection	8559173
85283649	85283672	12	+	27260156	PC3	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.19598020350366208	[459]	[294,425,1066,587]	4	hSpCas9	negative selection	8623453
85301245	85301268	12	-	27260156	PC3	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.21010666395034505	[437]	[225,338,737,380]	-5	hSpCas9	negative selection	8623454
85283614	85283637	12	-	27260156	PC3	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.01439742277486121	[506]	[262,547,810,604]	0	hSpCas9	negative selection	8623455
85280435	85280458	12	+	27260156	PATU8988T	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.6035710016098353	[341]	[319,538,637,513]	8	hSpCas9	negative selection	8680422
85301411	85301434	12	-	27260156	PATU8988T	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.09842987922560287	[996]	[756,1242,830,1341]	1	hSpCas9	negative selection	8680423
85280336	85280359	12	+	27260156	PATU8988T	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	1.222202310996138	[183]	[763,296,238,344]	9	hSpCas9	negative selection	8680424
85283649	85283672	12	+	27260156	PATU8988T	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.1358248566069105	[459]	[451,493,406,611]	2	hSpCas9	negative selection	8744704
85301245	85301268	12	-	27260156	PATU8988T	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.2437970244227991	[437]	[295,275,433,419]	-4	hSpCas9	negative selection	8744705
85283614	85283637	12	-	27260156	PATU8988T	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.23088331454405592	[506]	[629,641,582,439]	4	hSpCas9	negative selection	8744706
85280435	85280458	12	+	27260156	T47D	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.5248536371285102	[341]	[608,547,590,545]	9	hSpCas9	negative selection	8801673
85301411	85301434	12	-	27260156	T47D	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.06974327940161373	[996]	[1210,1154,1152,1367]	1	hSpCas9	negative selection	8801674
85280336	85280359	12	+	27260156	T47D	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.36465329603374674	[183]	[212,299,270,318]	7	hSpCas9	negative selection	8801675
85283649	85283672	12	+	27260156	T47D	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.27242211369874586	[459]	[637,614,648,690]	6	hSpCas9	negative selection	8865955
85301245	85301268	12	-	27260156	T47D	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.26686511374118305	[437]	[490,400,426,377]	-5	hSpCas9	negative selection	8865956
85283614	85283637	12	-	27260156	T47D	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.32409278284939064	[506]	[876,727,675,676]	7	hSpCas9	negative selection	8865957
85280435	85280458	12	+	27260156	SU8686	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.48989560608451815	[341]	[449,526,427,493]	7	hSpCas9	negative selection	8922924
85301411	85301434	12	-	27260156	SU8686	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.3262253940862867	[996]	[1032,1015,1495,1505]	5	hSpCas9	negative selection	8922925
85280336	85280359	12	+	27260156	SU8686	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.9022386665356896	[183]	[260,269,371,503]	9	hSpCas9	negative selection	8922926
85283649	85283672	12	+	27260156	SU8686	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.06304091540198223	[459]	[436,497,506,459]	1	hSpCas9	negative selection	8987206
85301245	85301268	12	-	27260156	SU8686	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.2826620116761766	[437]	[307,314,478,333]	-5	hSpCas9	negative selection	8987207
85283614	85283637	12	-	27260156	SU8686	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.13519700615561683	[506]	[409,489,737,603]	2	hSpCas9	negative selection	8987208
85280435	85280458	12	+	27260156	SKES1	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.27923824972275296	[341]	[522,459,358,339]	6	hSpCas9	negative selection	9044175
85301411	85301434	12	-	27260156	SKES1	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	-0.0050801320283173634	[996]	[1247,1108,951,726]	0	hSpCas9	negative selection	9044176
85280336	85280359	12	+	27260156	SKES1	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.24200533536761437	[183]	[237,202,195,228]	5	hSpCas9	negative selection	9044177
85283649	85283672	12	+	27260156	SKES1	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.13376190045826813	[459]	[455,635,424,501]	3	hSpCas9	negative selection	9108457
85301245	85301268	12	-	27260156	SKES1	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.2530841410222015	[437]	[447,406,287,339]	-5	hSpCas9	negative selection	9108458
85283614	85283637	12	-	27260156	SKES1	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.38438851123716944	[506]	[689,682,730,548]	7	hSpCas9	negative selection	9108459
85280435	85280458	12	+	27260156	TOV112D	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.28332991778511185	[341]	[356,425,501,379]	5	hSpCas9	negative selection	9165426
85301411	85301434	12	-	27260156	TOV112D	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.21728523303301728	[996]	[1661,1438,883,777]	4	hSpCas9	negative selection	9165427
85280336	85280359	12	+	27260156	TOV112D	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.3130511376102505	[183]	[264,226,89,332]	6	hSpCas9	negative selection	9165428
85283649	85283672	12	+	27260156	TOV112D	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.2888363274935709	[459]	[679,672,502,434]	5	hSpCas9	negative selection	9229708
85301245	85301268	12	-	27260156	TOV112D	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	0.25866639330257213	[437]	[575,486,598,433]	5	hSpCas9	negative selection	9229709
85283614	85283637	12	-	27260156	TOV112D	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.37706152769236073	[506]	[825,1027,464,428]	7	hSpCas9	negative selection	9229710
85280435	85280458	12	+	27260156	TC71	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.3892293680220719	[341]	[458,425,419,626]	8	hSpCas9	negative selection	9286677
85301411	85301434	12	-	27260156	TC71	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.0712491042784884	[996]	[1070,1110,1128,1184]	1	hSpCas9	negative selection	9286678
85280336	85280359	12	+	27260156	TC71	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.43714679592726224	[183]	[200,308,279,279]	8	hSpCas9	negative selection	9286679
85283649	85283672	12	+	27260156	TC71	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.10172421578316412	[459]	[452,622,479,565]	2	hSpCas9	negative selection	9350959
85301245	85301268	12	-	27260156	TC71	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.20182548085968371	[437]	[372,305,404,558]	-5	hSpCas9	negative selection	9350960
85283614	85283637	12	-	27260156	TC71	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.09317421944253593	[506]	[435,671,486,739]	2	hSpCas9	negative selection	9350961
85280435	85280458	12	+	27260156	TC32	viability	CGCCGAGCATCACGTGCGCTTGG	ALX1	ENSG00000180318	0.8020645149077168	[341]	[550,461,739,520]	9	hSpCas9	negative selection	9407928
85301411	85301434	12	-	27260156	TC32	viability	CATTCGAAGAACTGCGATACTGG	ALX1	ENSG00000180318	0.30406475193737315	[996]	[1083,777,1399,1512]	5	hSpCas9	negative selection	9407929
85280336	85280359	12	+	27260156	TC32	viability	AGGTCCTCTGGAGCACGTTATGG	ALX1	ENSG00000180318	0.5067188245518119	[183]	[194,219,314,266]	8	hSpCas9	negative selection	9407930
85283649	85283672	12	+	27260156	TC32	viability	CTCCGAATGTCTCCCGTGAAAGG	ALX1	ENSG00000180318	0.5702877413671259	[459]	[505,478,700,950]	8	hSpCas9	negative selection	9472210
85301245	85301268	12	-	27260156	TC32	viability	GTCCGAGGCGAGTGAGAATAAGG	ALX1	ENSG00000180318	-0.2707470953366583	[437]	[249,222,459,498]	-5	hSpCas9	negative selection	9472211
85283614	85283637	12	-	27260156	TC32	viability	GTCCATAGCTCTATTCAGTTCGG	ALX1	ENSG00000180318	0.08148039392832085	[506]	[540,362,521,617]	1	hSpCas9	negative selection	9472212
85280345	85280368	12	+	27869803	HPAFII	viability after 27 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.358697693322409	[218]	[99,457,NaN]	9	hSpCas9	negative selection	9548739
85280469	85280492	12	-	27869803	HPAFII	viability after 27 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	-0.18607632741986357	[335]	[151,157,NaN]	-1	hSpCas9	negative selection	9548740
85283650	85283673	12	+	27869803	HPAFII	viability after 27 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.29966107639070594	[532]	[327,358,NaN]	2	hSpCas9	negative selection	9548741
85283866	85283889	12	-	27869803	HPAFII	viability after 27 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.2409465357596417	[337]	[251,174,NaN]	2	hSpCas9	negative selection	9548742
85301263	85301286	12	+	27869803	HPAFII	viability after 27 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.06370232852318747	[569]	[385,203,NaN]	0	hSpCas9	negative selection	9548743
85301264	85301287	12	+	27869803	HPAFII	viability after 27 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.13926851581044603	[258]	[109,134,NaN]	-1	hSpCas9	negative selection	9548744
85280345	85280368	12	+	27869803	HPAFII	viability after 15 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.7355127758136814	[218]	[373,402,NaN]	7	hSpCas9	negative selection	9631188
85280469	85280492	12	-	27869803	HPAFII	viability after 15 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.33589382572925025	[335]	[567,385,NaN]	4	hSpCas9	negative selection	9631189
85283650	85283673	12	+	27869803	HPAFII	viability after 15 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.2680875268380629	[532]	[785,629,NaN]	3	hSpCas9	negative selection	9631190
85283866	85283889	12	-	27869803	HPAFII	viability after 15 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.2306275532240865	[337]	[543,352,NaN]	2	hSpCas9	negative selection	9631191
85301263	85301286	12	+	27869803	HPAFII	viability after 15 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.11803095473273983	[569]	[715,632,NaN]	1	hSpCas9	negative selection	9631192
85301264	85301287	12	+	27869803	HPAFII	viability after 15 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.5887336114695306	[258]	[335,469,NaN]	6	hSpCas9	negative selection	9631193
85280345	85280368	12	+	27869803	ASPC1	viability after 36 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.06302854174866065	[654]	[212,309,197]	0	hSpCas9	negative selection	9713637
85280469	85280492	12	-	27869803	ASPC1	viability after 36 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.94297956777751	[1075]	[1401,136,701]	8	hSpCas9	negative selection	9713638
85283650	85283673	12	+	27869803	ASPC1	viability after 36 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	-0.03597060699750587	[1670]	[734,469,524]	0	hSpCas9	negative selection	9713639
85283866	85283889	12	-	27869803	ASPC1	viability after 36 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.12084272199149598	[745]	[136,404,285]	1	hSpCas9	negative selection	9713640
85301263	85301286	12	+	27869803	ASPC1	viability after 36 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.1005802524807109	[2012]	[896,614,510]	-1	hSpCas9	negative selection	9713641
85301264	85301287	12	+	27869803	ASPC1	viability after 36 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.16250433861312297	[986]	[258,352,302]	-2	hSpCas9	negative selection	9713642
85280345	85280368	12	+	27869803	PATU8988S	viability after 35 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.27224724254542804	[512]	[205,135,NaN]	-3	hSpCas9	negative selection	9796086
85280469	85280492	12	-	27869803	PATU8988S	viability after 35 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.26520152792455354	[938]	[454,453,NaN]	3	hSpCas9	negative selection	9796087
85283650	85283673	12	+	27869803	PATU8988S	viability after 35 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.5432338654803321	[1490]	[905,843,NaN]	6	hSpCas9	negative selection	9796088
85283866	85283889	12	-	27869803	PATU8988S	viability after 35 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	-0.2274143693044967	[576]	[196,199,NaN]	-2	hSpCas9	negative selection	9796089
85301263	85301286	12	+	27869803	PATU8988S	viability after 35 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.28709737956339687	[1330]	[363,514,NaN]	-3	hSpCas9	negative selection	9796090
85301264	85301287	12	+	27869803	PATU8988S	viability after 35 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.2616427170464187	[754]	[381,346,NaN]	3	hSpCas9	negative selection	9796091
85280345	85280368	12	+	27869803	PATU8988S	viability after 31 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-1.0860450888453421	[512]	[76,120,NaN]	-8	hSpCas9	negative selection	9878535
85280469	85280492	12	-	27869803	PATU8988S	viability after 31 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.10567533187785816	[938]	[379,431,NaN]	1	hSpCas9	negative selection	9878536
85283650	85283673	12	+	27869803	PATU8988S	viability after 31 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.4460716955729207	[1490]	[706,940,NaN]	5	hSpCas9	negative selection	9878537
85283866	85283889	12	-	27869803	PATU8988S	viability after 31 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.500779533087509	[576]	[281,380,NaN]	6	hSpCas9	negative selection	9878538
85301263	85301286	12	+	27869803	PATU8988S	viability after 31 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.11765540882389192	[1330]	[310,715,NaN]	-1	hSpCas9	negative selection	9878539
85301264	85301287	12	+	27869803	PATU8988S	viability after 31 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.24282316674896331	[754]	[335,381,NaN]	3	hSpCas9	negative selection	9878540
85280345	85280368	12	+	27869803	HPAFII	viability after 35 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.4279235724585588	[218]	[72,551,NaN]	8	hSpCas9	negative selection	9960984
85280469	85280492	12	-	27869803	HPAFII	viability after 35 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.26239212786070515	[335]	[140,274,NaN]	1	hSpCas9	negative selection	9960985
85283650	85283673	12	+	27869803	HPAFII	viability after 35 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.6396044593207006	[532]	[449,386,NaN]	4	hSpCas9	negative selection	9960986
85283866	85283889	12	-	27869803	HPAFII	viability after 35 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.3833614560023623	[337]	[257,183,NaN]	2	hSpCas9	negative selection	9960987
85301263	85301286	12	+	27869803	HPAFII	viability after 35 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	0.23348805583437504	[569]	[255,432,NaN]	1	hSpCas9	negative selection	9960988
85301264	85301287	12	+	27869803	HPAFII	viability after 35 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	0.2828894985016509	[258]	[143,176,NaN]	1	hSpCas9	negative selection	9960989
85280345	85280368	12	+	27869803	HPAFII	viability after 31 days	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.0326709005216392	[218]	[37,483,NaN]	7	hSpCas9	negative selection	10043433
85280469	85280492	12	-	27869803	HPAFII	viability after 31 days	TCACCGCTGCTGTCCTGACAGGG	ALX1	ENSG00000180318	0.2524055320725483	[335]	[204,245,NaN]	1	hSpCas9	negative selection	10043434
85283650	85283673	12	+	27869803	HPAFII	viability after 31 days	TCCGAATGTCTCCCGTGAAAGGG	ALX1	ENSG00000180318	0.6690493821892314	[532]	[522,423,NaN]	5	hSpCas9	negative selection	10043435
85283866	85283889	12	-	27869803	HPAFII	viability after 31 days	TTTGGCTCCTACCTGGACCCTGG	ALX1	ENSG00000180318	0.09853819280186399	[337]	[249,151,NaN]	0	hSpCas9	negative selection	10043436
85301263	85301286	12	+	27869803	HPAFII	viability after 31 days	CGGACAGATTCCAGTTACACGGG	ALX1	ENSG00000180318	-0.017600941379163793	[569]	[360,266,NaN]	0	hSpCas9	negative selection	10043437
85301264	85301287	12	+	27869803	HPAFII	viability after 31 days	GGACAGATTCCAGTTACACGGGG	ALX1	ENSG00000180318	-0.01967960471153176	[258]	[151,133,NaN]	0	hSpCas9	negative selection	10043438
85280345	85280368	12	+	28162770	P31FUJ	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.10316692399778317	[233]	[255]	-1	hSpCas9	negative selection	10109776
85283684	85283707	12	+	28162770	P31FUJ	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.5508442907305138	[297]	[512]	6	hSpCas9	negative selection	10109777
85283725	85283748	12	+	28162770	P31FUJ	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.20015720286172733	[19]	[26]	2	hSpCas9	negative selection	10109778
85280431	85280454	12	-	28162770	P31FUJ	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.6422711836847909	[216]	[397]	7	hSpCas9	negative selection	10109779
85280466	85280489	12	+	28162770	P31FUJ	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.41593620674009224	[117]	[184]	4	hSpCas9	negative selection	10109780
85283849	85283872	12	+	28162770	P31FUJ	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5721818131804786	[173]	[303]	6	hSpCas9	negative selection	10109781
85286960	85286983	12	+	28162770	P31FUJ	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.5017568920447326	[719]	[1197]	5	hSpCas9	negative selection	10109782
85280313	85280336	12	+	28162770	P31FUJ	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.5223107364620869	[236]	[399]	6	hSpCas9	negative selection	10109783
85283663	85283686	12	+	28162770	P31FUJ	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.6614585420460415	[282]	[525]	7	hSpCas9	negative selection	10109784
85280393	85280416	12	+	28162770	P31FUJ	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.6358445560540009	[315]	[576]	7	hSpCas9	negative selection	10109785
85280345	85280368	12	+	28162770	TF1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	NaN	[NaN]	[254]	1	hSpCas9	negative selection	10279142
85283684	85283707	12	+	28162770	TF1	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	NaN	[NaN]	[271]	1	hSpCas9	negative selection	10279143
85283725	85283748	12	+	28162770	TF1	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	NaN	[NaN]	[9]	1	hSpCas9	negative selection	10279144
85280431	85280454	12	-	28162770	TF1	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	NaN	[NaN]	[148]	1	hSpCas9	negative selection	10279145
85280466	85280489	12	+	28162770	TF1	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	NaN	[NaN]	[111]	1	hSpCas9	negative selection	10279146
85283849	85283872	12	+	28162770	TF1	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	NaN	[NaN]	[279]	1	hSpCas9	negative selection	10279147
85286960	85286983	12	+	28162770	TF1	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	NaN	[NaN]	[721]	1	hSpCas9	negative selection	10279148
85280313	85280336	12	+	28162770	TF1	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	NaN	[NaN]	[287]	1	hSpCas9	negative selection	10279149
85283663	85283686	12	+	28162770	TF1	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	NaN	[NaN]	[258]	1	hSpCas9	negative selection	10279150
85280393	85280416	12	+	28162770	TF1	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	NaN	[NaN]	[469]	1	hSpCas9	negative selection	10279151
85280345	85280368	12	+	28162770	SKM1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.17799830197799454	[52]	[105]	1	hSpCas9	negative selection	10448508
85283684	85283707	12	+	28162770	SKM1	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	-0.29274862988713685	[87]	[126]	-2	hSpCas9	negative selection	10448509
85283725	85283748	12	+	28162770	SKM1	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	2.2368919910315634	[5]	[49]	9	hSpCas9	negative selection	10448510
85280431	85280454	12	-	28162770	SKM1	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.4259258154215799	[63]	[151]	4	hSpCas9	negative selection	10448511
85280466	85280489	12	+	28162770	SKM1	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.3408750082853892	[47]	[66]	-3	hSpCas9	negative selection	10448512
85283849	85283872	12	+	28162770	SKM1	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5136013337624333	[62]	[158]	5	hSpCas9	negative selection	10448513
85286960	85286983	12	+	28162770	SKM1	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.6042630566800924	[159]	[429]	6	hSpCas9	negative selection	10448514
85280313	85280336	12	+	28162770	SKM1	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.9853532240355984	[35]	[125]	8	hSpCas9	negative selection	10448515
85283663	85283686	12	+	28162770	SKM1	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.2510617641677179	[76]	[161]	2	hSpCas9	negative selection	10448516
85280393	85280416	12	+	28162770	SKM1	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.21406755668551625	[78]	[161]	2	hSpCas9	negative selection	10448517
85280345	85280368	12	+	28162770	SKM1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.17799830197799454	[52]	[105]	1	hSpCas9	negative selection	10617874
85283684	85283707	12	+	28162770	SKM1	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	-0.29274862988713685	[87]	[126]	-2	hSpCas9	negative selection	10617875
85283725	85283748	12	+	28162770	SKM1	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	2.2368919910315634	[5]	[49]	9	hSpCas9	negative selection	10617876
85280431	85280454	12	-	28162770	SKM1	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.4259258154215799	[63]	[151]	4	hSpCas9	negative selection	10617877
85280466	85280489	12	+	28162770	SKM1	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.3408750082853892	[47]	[66]	-3	hSpCas9	negative selection	10617878
85283849	85283872	12	+	28162770	SKM1	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5136013337624333	[62]	[158]	5	hSpCas9	negative selection	10617879
85286960	85286983	12	+	28162770	SKM1	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.6042630566800924	[159]	[429]	6	hSpCas9	negative selection	10617880
85280313	85280336	12	+	28162770	SKM1	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.9853532240355984	[35]	[125]	8	hSpCas9	negative selection	10617881
85283663	85283686	12	+	28162770	SKM1	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.2510617641677179	[76]	[161]	2	hSpCas9	negative selection	10617882
85280393	85280416	12	+	28162770	SKM1	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.21406755668551625	[78]	[161]	2	hSpCas9	negative selection	10617883
85280345	85280368	12	+	28162770	PL21	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.0289529644830966	[142]	[126]	8	hSpCas9	negative selection	10787240
85283684	85283707	12	+	28162770	PL21	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.7611334673841454	[262]	[193]	6	hSpCas9	negative selection	10787241
85283725	85283748	12	+	28162770	PL21	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.3927846924317162	[27]	[15]	3	hSpCas9	negative selection	10787242
85280431	85280454	12	-	28162770	PL21	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.6874913185840542	[213]	[149]	6	hSpCas9	negative selection	10787243
85280466	85280489	12	+	28162770	PL21	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.5283722862308913	[136]	[85]	4	hSpCas9	negative selection	10787244
85283849	85283872	12	+	28162770	PL21	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.23051326353283885	[187]	[95]	2	hSpCas9	negative selection	10787245
85286960	85286983	12	+	28162770	PL21	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	-0.262152792396285	[630]	[228]	-2	hSpCas9	negative selection	10787246
85280313	85280336	12	+	28162770	PL21	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	1.359338209338574	[179]	[200]	9	hSpCas9	negative selection	10787247
85283663	85283686	12	+	28162770	PL21	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.23130054597578067	[228]	[116]	2	hSpCas9	negative selection	10787248
85280393	85280416	12	+	28162770	PL21	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.23001014407145792	[238]	[121]	2	hSpCas9	negative selection	10787249
85280345	85280368	12	+	28162770	P31FUJ	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	-0.10316692399778317	[233]	[255]	-1	hSpCas9	negative selection	10956606
85283684	85283707	12	+	28162770	P31FUJ	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.5508442907305138	[297]	[512]	6	hSpCas9	negative selection	10956607
85283725	85283748	12	+	28162770	P31FUJ	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.20015720286172733	[19]	[26]	2	hSpCas9	negative selection	10956608
85280431	85280454	12	-	28162770	P31FUJ	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.6422711836847909	[216]	[397]	7	hSpCas9	negative selection	10956609
85280466	85280489	12	+	28162770	P31FUJ	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.41593620674009224	[117]	[184]	4	hSpCas9	negative selection	10956610
85283849	85283872	12	+	28162770	P31FUJ	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5721818131804786	[173]	[303]	6	hSpCas9	negative selection	10956611
85286960	85286983	12	+	28162770	P31FUJ	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.5017568920447326	[719]	[1197]	5	hSpCas9	negative selection	10956612
85280313	85280336	12	+	28162770	P31FUJ	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.5223107364620869	[236]	[399]	6	hSpCas9	negative selection	10956613
85283663	85283686	12	+	28162770	P31FUJ	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.6614585420460415	[282]	[525]	7	hSpCas9	negative selection	10956614
85280393	85280416	12	+	28162770	P31FUJ	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.6358445560540009	[315]	[576]	7	hSpCas9	negative selection	10956615
85280345	85280368	12	+	28162770	OCIAML5	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.07954645415575279	[107]	[205]	0	hSpCas9	negative selection	11125972
85283684	85283707	12	+	28162770	OCIAML5	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.3642513360627665	[169]	[394]	3	hSpCas9	negative selection	11125973
85283725	85283748	12	+	28162770	OCIAML5	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.5953924061072242	[10]	[29]	5	hSpCas9	negative selection	11125974
85280431	85280454	12	-	28162770	OCIAML5	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.7643088824886207	[105]	[324]	6	hSpCas9	negative selection	11125975
85280466	85280489	12	+	28162770	OCIAML5	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.7765228622574173	[75]	[234]	6	hSpCas9	negative selection	11125976
85283849	85283872	12	+	28162770	OCIAML5	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.4409804040362364	[110]	[271]	4	hSpCas9	negative selection	11125977
85286960	85286983	12	+	28162770	OCIAML5	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.21953816993912523	[451]	[949]	1	hSpCas9	negative selection	11125978
85280313	85280336	12	+	28162770	OCIAML5	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.43906837047776237	[160]	[393]	4	hSpCas9	negative selection	11125979
85283663	85283686	12	+	28162770	OCIAML5	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.4432610252966679	[231]	[307]	-3	hSpCas9	negative selection	11125980
85280393	85280416	12	+	28162770	OCIAML5	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	1.2342005015314057	[141]	[602]	9	hSpCas9	negative selection	11125981
85280345	85280368	12	+	28162770	EOL1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.96957169625653	[346]	[715]	9	hSpCas9	negative selection	11295338
85283684	85283707	12	+	28162770	EOL1	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.9987864018624532	[302]	[318]	8	hSpCas9	negative selection	11295339
85283725	85283748	12	+	28162770	EOL1	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.05383078851550094	[63]	[34]	0	hSpCas9	negative selection	11295340
85280431	85280454	12	-	28162770	EOL1	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.3537991300690793	[403]	[271]	3	hSpCas9	negative selection	11295341
85280466	85280489	12	+	28162770	EOL1	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.7522322227032282	[301]	[267]	6	hSpCas9	negative selection	11295342
85283849	85283872	12	+	28162770	EOL1	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.5158741778065523	[376]	[283]	4	hSpCas9	negative selection	11295343
85286960	85286983	12	+	28162770	EOL1	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.8315779535601684	[688]	[645]	7	hSpCas9	negative selection	11295344
85280313	85280336	12	+	28162770	EOL1	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.5924032806400354	[247]	[196]	5	hSpCas9	negative selection	11295345
85283663	85283686	12	+	28162770	EOL1	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.07982599704844545	[263]	[146]	0	hSpCas9	negative selection	11295346
85280393	85280416	12	+	28162770	EOL1	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.09949084777947148	[286]	[161]	0	hSpCas9	negative selection	11295347
85280345	85280368	12	+	28162770	MOLM13	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.8180757364734546	[272]	[347]	6	hSpCas9	negative selection	11464704
85283684	85283707	12	+	28162770	MOLM13	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	-0.49626541950869907	[317]	[162]	-3	hSpCas9	negative selection	11464705
85283725	85283748	12	+	28162770	MOLM13	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.6484616271863999	[14]	[16]	5	hSpCas9	negative selection	11464706
85280431	85280454	12	-	28162770	MOLM13	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.5014977741873985	[296]	[303]	4	hSpCas9	negative selection	11464707
85280466	85280489	12	+	28162770	MOLM13	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	1.0990748990263808	[214]	[332]	8	hSpCas9	negative selection	11464708
85283849	85283872	12	+	28162770	MOLM13	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	1.1233250902141865	[272]	[429]	8	hSpCas9	negative selection	11464709
85286960	85286983	12	+	28162770	MOLM13	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.6043784706757411	[775]	[852]	4	hSpCas9	negative selection	11464710
85280313	85280336	12	+	28162770	MOLM13	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.8376460015225563	[266]	[344]	6	hSpCas9	negative selection	11464711
85283663	85283686	12	+	28162770	MOLM13	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.6021011671779037	[358]	[393]	4	hSpCas9	negative selection	11464712
85280393	85280416	12	+	28162770	MOLM13	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.33832974777566277	[325]	[297]	2	hSpCas9	negative selection	11464713
85280345	85280368	12	+	28162770	HEL	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.3164234198720555	[246]	[196]	2	hSpCas9	negative selection	11634070
85283684	85283707	12	+	28162770	HEL	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.6310570269527217	[247]	[245]	5	hSpCas9	negative selection	11634071
85283725	85283748	12	+	28162770	HEL	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.6273503313673872	[40]	[16]	-4	hSpCas9	negative selection	11634072
85280431	85280454	12	-	28162770	HEL	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.4670591558322375	[313]	[277]	4	hSpCas9	negative selection	11634073
85280466	85280489	12	+	28162770	HEL	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.12017286841706809	[180]	[125]	1	hSpCas9	negative selection	11634074
85283849	85283872	12	+	28162770	HEL	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.7688476011501798	[207]	[226]	6	hSpCas9	negative selection	11634075
85286960	85286983	12	+	28162770	HEL	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.6334009674210208	[619]	[615]	5	hSpCas9	negative selection	11634076
85280313	85280336	12	+	28162770	HEL	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.7414231068597638	[225]	[241]	6	hSpCas9	negative selection	11634077
85283663	85283686	12	+	28162770	HEL	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.12963209829704803	[274]	[160]	-1	hSpCas9	negative selection	11634078
85280393	85280416	12	+	28162770	HEL	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.857529010380513	[298]	[346]	7	hSpCas9	negative selection	11634079
85280345	85280368	12	+	28162770	MV411	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.9908726402785393	[177]	[297]	8	hSpCas9	negative selection	11803436
85283684	85283707	12	+	28162770	MV411	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.4748480468858479	[245]	[287]	4	hSpCas9	negative selection	11803437
85283725	85283748	12	+	28162770	MV411	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	0.147901877231861	[29]	[27]	1	hSpCas9	negative selection	11803438
85280431	85280454	12	-	28162770	MV411	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	1.0911343199041	[223]	[401]	8	hSpCas9	negative selection	11803439
85280466	85280489	12	+	28162770	MV411	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.4379268468962185	[118]	[73]	-3	hSpCas9	negative selection	11803440
85283849	85283872	12	+	28162770	MV411	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	-0.3342498179055199	[219]	[146]	-3	hSpCas9	negative selection	11803441
85286960	85286983	12	+	28162770	MV411	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.8181581289281525	[508]	[755]	7	hSpCas9	negative selection	11803442
85280313	85280336	12	+	28162770	MV411	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.06740252169562175	[178]	[157]	0	hSpCas9	negative selection	11803443
85283663	85283686	12	+	28162770	MV411	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.34088211490183895	[268]	[286]	3	hSpCas9	negative selection	11803444
85280393	85280416	12	+	28162770	MV411	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	1.0336582597510353	[166]	[287]	8	hSpCas9	negative selection	11803445
85280345	85280368	12	+	28162770	MONOMAC1	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.8029631529404464	[111]	[223]	7	hSpCas9	negative selection	11972802
85283684	85283707	12	+	28162770	MONOMAC1	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	-0.29501200662285126	[197]	[184]	-3	hSpCas9	negative selection	11972803
85283725	85283748	12	+	28162770	MONOMAC1	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.27949900725152643	[17]	[16]	-2	hSpCas9	negative selection	11972804
85280431	85280454	12	-	28162770	MONOMAC1	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.4309943755534885	[153]	[237]	4	hSpCas9	negative selection	11972805
85280466	85280489	12	+	28162770	MONOMAC1	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	0.23310754460949848	[94]	[127]	2	hSpCas9	negative selection	11972806
85283849	85283872	12	+	28162770	MONOMAC1	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.2724484362416666	[129]	[179]	2	hSpCas9	negative selection	11972807
85286960	85286983	12	+	28162770	MONOMAC1	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	-0.007038431097534215	[340]	[388]	0	hSpCas9	negative selection	11972808
85280313	85280336	12	+	28162770	MONOMAC1	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	0.3704382269327615	[82]	[122]	3	hSpCas9	negative selection	11972809
85283663	85283686	12	+	28162770	MONOMAC1	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.09077815966899622	[156]	[168]	-1	hSpCas9	negative selection	11972810
85280393	85280416	12	+	28162770	MONOMAC1	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	-0.01592416498478283	[156]	[177]	0	hSpCas9	negative selection	11972811
85280345	85280368	12	+	28162770	OCIAML2	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	0.6473673841562904	[43]	[381]	6	hSpCas9	negative selection	12142168
85283684	85283707	12	+	28162770	OCIAML2	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.6818605828641304	[36]	[328]	6	hSpCas9	negative selection	12142169
85283725	85283748	12	+	28162770	OCIAML2	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-0.14870173035479883	[0]	[4]	-1	hSpCas9	negative selection	12142170
85280431	85280454	12	-	28162770	OCIAML2	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.6992951762001511	[45]	[413]	6	hSpCas9	negative selection	12142171
85280466	85280489	12	+	28162770	OCIAML2	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.799252572703532	[26]	[85]	-5	hSpCas9	negative selection	12142172
85283849	85283872	12	+	28162770	OCIAML2	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.3607705707820939	[33]	[241]	3	hSpCas9	negative selection	12142173
85286960	85286983	12	+	28162770	OCIAML2	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.261174063808266	[97]	[650]	2	hSpCas9	negative selection	12142174
85280313	85280336	12	+	28162770	OCIAML2	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	-0.03929051363421694	[32]	[177]	0	hSpCas9	negative selection	12142175
85283663	85283686	12	+	28162770	OCIAML2	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	0.325541358322767	[36]	[256]	3	hSpCas9	negative selection	12142176
85280393	85280416	12	+	28162770	OCIAML2	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.6459140798721276	[51]	[450]	6	hSpCas9	negative selection	12142177
85280345	85280368	12	+	28162770	OCIAML3	viability	GGAGCACGTTATGGAGACGCTGG	ALX1	ENSG00000180318	1.0143377878349438	[50]	[310]	8	hSpCas9	negative selection	12311534
85283684	85283707	12	+	28162770	OCIAML3	viability	GGGAGAGCTGGATGAACTTGGGG	ALX1	ENSG00000180318	0.7147450658154659	[43]	[217]	6	hSpCas9	negative selection	12311535
85283725	85283748	12	+	28162770	OCIAML3	viability	TATCCAGCAGTAAGAAACGGAGG	ALX1	ENSG00000180318	-1.442004546879113	[8]	[9]	-7	hSpCas9	negative selection	12311536
85280431	85280454	12	-	28162770	OCIAML3	viability	GCGCACGTGATGCTCGGCGCGGG	ALX1	ENSG00000180318	0.5496858195449699	[73]	[326]	5	hSpCas9	negative selection	12311537
85280466	85280489	12	+	28162770	OCIAML3	viability	TCGCCCTGTCAGGACAGCAGCGG	ALX1	ENSG00000180318	-0.2995602818589078	[52]	[129]	-2	hSpCas9	negative selection	12311538
85283849	85283872	12	+	28162770	OCIAML3	viability	GACAGAGCTCACTGAGGCCAGGG	ALX1	ENSG00000180318	0.26848883592590195	[54]	[199]	2	hSpCas9	negative selection	12311539
85286960	85286983	12	+	28162770	OCIAML3	viability	AAGGACTGACAGCTACCCACAGG	ALX1	ENSG00000180318	0.13330778380832364	[134]	[446]	1	hSpCas9	negative selection	12311540
85280313	85280336	12	+	28162770	OCIAML3	viability	AGTGACTTTTACATGGGCGCAGG	ALX1	ENSG00000180318	1.0758437579835058	[43]	[279]	8	hSpCas9	negative selection	12311541
85283663	85283686	12	+	28162770	OCIAML3	viability	CGTGAAAGGGATGCAAGAGAAGG	ALX1	ENSG00000180318	-0.07647306712329766	[72]	[208]	0	hSpCas9	negative selection	12311542
85280393	85280416	12	+	28162770	OCIAML3	viability	GTCTGCAGGCAAATGCGTGCAGG	ALX1	ENSG00000180318	0.619913264944833	[60]	[282]	5	hSpCas9	negative selection	12311543