Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:8911
• Symbol:CACNA1I
• Description:calcium voltage-gated channel subunit alpha1 I

DataSource: http://genomecrispr.dkfz.de/#!/results/CACNA1I

Export (tab separated) Export to Excel

Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
39649689	39649712	22	+	26472758	Jiyoye	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.1298526421515809	[147]	[50]	-6	hSpCas9	negative selection	22456
39646855	39646878	22	-	26472758	Jiyoye	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	1.4288704526051923	[32]	[66]	8	hSpCas9	negative selection	22457
39660416	39660439	22	+	26472758	Jiyoye	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.4687342707694827	[166]	[90]	-3	hSpCas9	negative selection	22458
39684489	39684512	22	-	26472758	Jiyoye	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.14183081498487837	[249]	[207]	1	hSpCas9	negative selection	22459
39570774	39570797	22	-	26472758	Jiyoye	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.5928246184941266	[237]	[118]	-4	hSpCas9	negative selection	22460
39600555	39600578	22	+	26472758	Jiyoye	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.4021410568850168	[183]	[104]	-3	hSpCas9	negative selection	22461
39634660	39634683	22	+	26472758	Jiyoye	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	1.1134441784491629	[75]	[123]	7	hSpCas9	negative selection	22462
39634689	39634712	22	+	26472758	Jiyoye	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.4071753815058735	[137]	[137]	3	hSpCas9	negative selection	22463
39642820	39642843	22	+	26472758	Jiyoye	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.3350255957500381	[40]	[38]	2	hSpCas9	negative selection	22464
39646838	39646861	22	-	26472758	Jiyoye	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.1244451743311599	[117]	[193]	7	hSpCas9	negative selection	22465
39649689	39649712	22	+	26472758	KBM7	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-0.9748018981697459	[715,664]	[214,120]	-6	hSpCas9	negative selection	213574
39646855	39646878	22	-	26472758	KBM7	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	-0.011797860567148856	[591,344]	[153,266]	0	hSpCas9	negative selection	213575
39660416	39660439	22	+	26472758	KBM7	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	0.39586209382501897	[535,379]	[434,140]	4	hSpCas9	negative selection	213576
39684489	39684512	22	-	26472758	KBM7	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.17792338994624313	[1013,556]	[339,298]	-2	hSpCas9	negative selection	213577
39570774	39570797	22	-	26472758	KBM7	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.7016514933352352	[797,527]	[176,198]	-5	hSpCas9	negative selection	213578
39600555	39600578	22	+	26472758	KBM7	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.4685709668133731	[954,738]	[342,233]	-4	hSpCas9	negative selection	213579
39634660	39634683	22	+	26472758	KBM7	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	0.3773768310741027	[415,571]	[306,308]	4	hSpCas9	negative selection	213580
39634689	39634712	22	+	26472758	KBM7	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.04127631337436344	[828,801]	[301,437]	0	hSpCas9	negative selection	213581
39642820	39642843	22	+	26472758	KBM7	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.8473570959276261	[229,256]	[67,60]	-6	hSpCas9	negative selection	213582
39646838	39646861	22	-	26472758	KBM7	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.28392737193178214	[441,355]	[141,302]	3	hSpCas9	negative selection	213583
39649689	39649712	22	+	26472758	Raji	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-3.8374749104339396	[307]	[4]	-9	hSpCas9	negative selection	404692
39646855	39646878	22	-	26472758	Raji	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.7068456057898707	[197]	[74]	6	hSpCas9	negative selection	404693
39660416	39660439	22	+	26472758	Raji	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	0.3942646831617611	[199]	[60]	4	hSpCas9	negative selection	404694
39684489	39684512	22	-	26472758	Raji	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.17549856029821154	[392]	[102]	1	hSpCas9	negative selection	404695
39570774	39570797	22	-	26472758	Raji	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	0.493992626325926	[204]	[66]	5	hSpCas9	negative selection	404696
39600555	39600578	22	+	26472758	Raji	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-1.4413863137241756	[432]	[36]	-7	hSpCas9	negative selection	404697
39634660	39634683	22	+	26472758	Raji	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.4022908380433195	[335]	[58]	-3	hSpCas9	negative selection	404698
39634689	39634712	22	+	26472758	Raji	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.3914223215978422	[389]	[68]	-3	hSpCas9	negative selection	404699
39642820	39642843	22	+	26472758	Raji	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.5193991410421837	[104]	[16]	-4	hSpCas9	negative selection	404700
39646838	39646861	22	-	26472758	Raji	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	-0.18019305472298597	[248]	[50]	-1	hSpCas9	negative selection	404701
39649689	39649712	22	+	26472758	K562	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.8774315317720025	[604]	[138]	-7	hSpCas9	negative selection	595810
39646855	39646878	22	-	26472758	K562	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.8621711635052725	[289]	[444]	7	hSpCas9	negative selection	595811
39660416	39660439	22	+	26472758	K562	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.9895927769368345	[373]	[158]	-5	hSpCas9	negative selection	595812
39684489	39684512	22	-	26472758	K562	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.08964919993002052	[579]	[520]	0	hSpCas9	negative selection	595813
39570774	39570797	22	-	26472758	K562	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-1.2566897217360649	[517]	[182]	-6	hSpCas9	negative selection	595814
39600555	39600578	22	+	26472758	K562	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-3.694807850061762	[766]	[49]	-9	hSpCas9	negative selection	595815
39634660	39634683	22	+	26472758	K562	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.679047424144254	[568]	[299]	-4	hSpCas9	negative selection	595816
39634689	39634712	22	+	26472758	K562	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.4426419606734454	[482]	[299]	-3	hSpCas9	negative selection	595817
39642820	39642843	22	+	26472758	K562	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.6257438346909715	[222]	[121]	-4	hSpCas9	negative selection	595818
39646838	39646861	22	-	26472758	K562	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.818921456742564	[322]	[480]	6	hSpCas9	negative selection	595819
39570956	39570979	22	+	26627737	DLD1	viability	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.6776585309281176	[0]	[0,0,0]	9	hSpCas9	negative selection	817917
39598181	39598204	22	-	26627737	DLD1	viability	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.31434103815074765	[261]	[156,90,62]	3	hSpCas9	negative selection	817918
39619397	39619420	22	-	26627737	DLD1	viability	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.723321553856475	[545]	[112,193,19]	-6	hSpCas9	negative selection	817919
39640977	39641000	22	-	26627737	DLD1	viability	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.1160315903535305	[1213]	[429,583,251]	1	hSpCas9	negative selection	817920
39646592	39646615	22	-	26627737	DLD1	viability	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.29274316642620307	[638]	[273,180,271]	3	hSpCas9	negative selection	817921
39647900	39647923	22	-	26627737	DLD1	viability	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.9121971382772032	[1052]	[191,203,135]	-7	hSpCas9	negative selection	817922
39570956	39570979	22	+	26627737	GBM cells	viability after 5 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8989138748856851	[0]	[0,0]	9	hSpCas9	negative selection	900232
39598181	39598204	22	-	26627737	GBM cells	viability after 5 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.5970869860548329	[97]	[73,84]	8	hSpCas9	negative selection	900233
39619397	39619420	22	-	26627737	GBM cells	viability after 5 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.03499693465654197	[191]	[107,92]	0	hSpCas9	negative selection	900234
39640977	39641000	22	-	26627737	GBM cells	viability after 5 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.22202888472349014	[485]	[312,294]	3	hSpCas9	negative selection	900235
39646592	39646615	22	-	26627737	GBM cells	viability after 5 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.33604200791467487	[345]	[163,129]	-5	hSpCas9	negative selection	900236
39647900	39647923	22	-	26627737	GBM cells	viability after 5 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.31985823077206543	[397]	[188,152]	-5	hSpCas9	negative selection	900237
39570956	39570979	22	+	26627737	GBM cells	viability after 13 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8821031235784469	[0]	[0,0]	9	hSpCas9	negative selection	982547
39598181	39598204	22	-	26627737	GBM cells	viability after 13 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.7077739360930971	[97]	[125,54]	8	hSpCas9	negative selection	982548
39619397	39619420	22	-	26627737	GBM cells	viability after 13 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.2851309383730678	[191]	[106,67]	-3	hSpCas9	negative selection	982549
39640977	39641000	22	-	26627737	GBM cells	viability after 13 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.14273054834155308	[485]	[293,193]	-2	hSpCas9	negative selection	982550
39646592	39646615	22	-	26627737	GBM cells	viability after 13 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.4579816322089874	[345]	[166,111]	-5	hSpCas9	negative selection	982551
39647900	39647923	22	-	26627737	GBM cells	viability after 13 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.12806088663868687	[397]	[233,167]	-1	hSpCas9	negative selection	982552
39570956	39570979	22	+	26627737	GBM cells	viability after 21 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8802332996156581	[0]	[0,0]	8	hSpCas9	negative selection	1064862
39598181	39598204	22	-	26627737	GBM cells	viability after 21 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	1.1319094595969075	[97]	[135,98]	9	hSpCas9	negative selection	1064863
39619397	39619420	22	-	26627737	GBM cells	viability after 21 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.013412176393040154	[191]	[106,99]	0	hSpCas9	negative selection	1064864
39640977	39641000	22	-	26627737	GBM cells	viability after 21 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.018270944562841396	[485]	[290,232]	0	hSpCas9	negative selection	1064865
39646592	39646615	22	-	26627737	GBM cells	viability after 21 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.12312897451905319	[345]	[222,187]	1	hSpCas9	negative selection	1064866
39647900	39647923	22	-	26627737	GBM cells	viability after 21 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.15235295393674486	[397]	[204,184]	-1	hSpCas9	negative selection	1064867
39570956	39570979	22	+	26627737	RPE1	viability after 9 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.6754465303148196	[0]	[0,0]	8	hSpCas9	negative selection	1147177
39598181	39598204	22	-	26627737	RPE1	viability after 9 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	4.998977916762881	[37]	[465,269]	9	hSpCas9	negative selection	1147178
39619397	39619420	22	-	26627737	RPE1	viability after 9 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.8560122060664527	[944]	[200,114]	-6	hSpCas9	negative selection	1147179
39640977	39641000	22	-	26627737	RPE1	viability after 9 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.41306324830888075	[1871]	[799,754]	3	hSpCas9	negative selection	1147180
39646592	39646615	22	-	26627737	RPE1	viability after 9 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.32478307072702284	[1136]	[330,593]	2	hSpCas9	negative selection	1147181
39647900	39647923	22	-	26627737	RPE1	viability after 9 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.898626521274283	[1752]	[265,330]	-6	hSpCas9	negative selection	1147182
39570956	39570979	22	+	26627737	RPE1	viability after 12 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.8961114116731377	[0]	[0,0]	8	hSpCas9	negative selection	1229492
39598181	39598204	22	-	26627737	RPE1	viability after 12 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	4.976410017017283	[37]	[351,288]	9	hSpCas9	negative selection	1229493
39619397	39619420	22	-	26627737	RPE1	viability after 12 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.7393182734547776	[944]	[103,202]	-5	hSpCas9	negative selection	1229494
39640977	39641000	22	-	26627737	RPE1	viability after 12 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.13053262632785034	[1871]	[552,547]	0	hSpCas9	negative selection	1229495
39646592	39646615	22	-	26627737	RPE1	viability after 12 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.0026451929794916174	[1136]	[284,325]	0	hSpCas9	negative selection	1229496
39647900	39647923	22	-	26627737	RPE1	viability after 12 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.6273741262483602	[1752]	[232,380]	-4	hSpCas9	negative selection	1229497
39570956	39570979	22	+	26627737	RPE1	viability after 15 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.8217488738718943	[0]	[0,0]	8	hSpCas9	negative selection	1311807
39598181	39598204	22	-	26627737	RPE1	viability after 15 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	5.111110047619297	[37]	[482,263]	9	hSpCas9	negative selection	1311808
39619397	39619420	22	-	26627737	RPE1	viability after 15 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	0.15661543235490022	[944]	[411,187]	1	hSpCas9	negative selection	1311809
39640977	39641000	22	-	26627737	RPE1	viability after 15 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.1550255843293542	[1871]	[835,351]	1	hSpCas9	negative selection	1311810
39646592	39646615	22	-	26627737	RPE1	viability after 15 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.03484717791885761	[1136]	[385,274]	0	hSpCas9	negative selection	1311811
39647900	39647923	22	-	26627737	RPE1	viability after 15 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.6232081069304947	[1752]	[268,372]	-4	hSpCas9	negative selection	1311812
39570956	39570979	22	+	26627737	RPE1	viability after 18 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.889668260100743	[0]	[0,0]	8	hSpCas9	negative selection	1394122
39598181	39598204	22	-	26627737	RPE1	viability after 18 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	4.67672871982719	[37]	[357,159]	9	hSpCas9	negative selection	1394123
39619397	39619420	22	-	26627737	RPE1	viability after 18 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.0491000302313149	[944]	[289,199]	0	hSpCas9	negative selection	1394124
39640977	39641000	22	-	26627737	RPE1	viability after 18 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.06337388219919227	[1871]	[538,515]	0	hSpCas9	negative selection	1394125
39646592	39646615	22	-	26627737	RPE1	viability after 18 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.24270347182224394	[1136]	[320,407]	1	hSpCas9	negative selection	1394126
39647900	39647923	22	-	26627737	RPE1	viability after 18 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.4771343938985917	[1752]	[262,421]	-3	hSpCas9	negative selection	1394127
39570956	39570979	22	+	26627737	HeLa	viability after 8 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.200473099817617	[0]	[0,0,0]	8	hSpCas9	negative selection	1476437
39598181	39598204	22	-	26627737	HeLa	viability after 8 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	1.2864102861826792	[173]	[417,70,120]	8	hSpCas9	negative selection	1476438
39619397	39619420	22	-	26627737	HeLa	viability after 8 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.2243407008229443	[261]	[202,342,240]	8	hSpCas9	negative selection	1476439
39640977	39641000	22	-	26627737	HeLa	viability after 8 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.8743373005082802	[651]	[488,730,345]	7	hSpCas9	negative selection	1476440
39646592	39646615	22	-	26627737	HeLa	viability after 8 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.19661790433864823	[570]	[272,328,251]	2	hSpCas9	negative selection	1476441
39647900	39647923	22	-	26627737	HeLa	viability after 8 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.34740309415468945	[673]	[264,245,190]	-3	hSpCas9	negative selection	1476442
39570956	39570979	22	+	26627737	HeLa	viability after 12 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.2748581272013126	[0]	[0,0,0]	8	hSpCas9	negative selection	1558752
39598181	39598204	22	-	26627737	HeLa	viability after 12 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	-0.291270918664587	[173]	[8,94,66]	-3	hSpCas9	negative selection	1558753
39619397	39619420	22	-	26627737	HeLa	viability after 12 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.0907600167547211	[261]	[174,260,250]	7	hSpCas9	negative selection	1558754
39640977	39641000	22	-	26627737	HeLa	viability after 12 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.7303966905872661	[651]	[478,456,404]	6	hSpCas9	negative selection	1558755
39646592	39646615	22	-	26627737	HeLa	viability after 12 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.12279486530394934	[570]	[236,362,158]	1	hSpCas9	negative selection	1558756
39647900	39647923	22	-	26627737	HeLa	viability after 12 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.33854126682823743	[673]	[281,197,184]	-3	hSpCas9	negative selection	1558757
39570956	39570979	22	+	26627737	HeLa	viability after 15 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.2965331123080048	[0]	[0,0,0]	8	hSpCas9	negative selection	1641067
39598181	39598204	22	-	26627737	HeLa	viability after 15 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	-0.5655479429386467	[173]	[47,78,13]	-5	hSpCas9	negative selection	1641068
39619397	39619420	22	-	26627737	HeLa	viability after 15 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.3054620220236082	[261]	[204,221,372]	8	hSpCas9	negative selection	1641069
39640977	39641000	22	-	26627737	HeLa	viability after 15 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.799097475908948	[651]	[301,739,335]	6	hSpCas9	negative selection	1641070
39646592	39646615	22	-	26627737	HeLa	viability after 15 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.03976142697596585	[570]	[237,337,91]	0	hSpCas9	negative selection	1641071
39647900	39647923	22	-	26627737	HeLa	viability after 15 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.22365787127806414	[673]	[139,284,284]	-2	hSpCas9	negative selection	1641072
39570956	39570979	22	+	26627737	HeLa	viability after 18 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.6067387639618715	[0]	[0,0,0]	9	hSpCas9	negative selection	1723382
39598181	39598204	22	-	26627737	HeLa	viability after 18 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	-0.6441645690631885	[173]	[25,66,13]	-5	hSpCas9	negative selection	1723383
39619397	39619420	22	-	26627737	HeLa	viability after 18 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.317772246740828	[261]	[140,184,372]	8	hSpCas9	negative selection	1723384
39640977	39641000	22	-	26627737	HeLa	viability after 18 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.7948785116251313	[651]	[198,613,335]	6	hSpCas9	negative selection	1723385
39646592	39646615	22	-	26627737	HeLa	viability after 18 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.010554014812834134	[570]	[163,293,91]	0	hSpCas9	negative selection	1723386
39647900	39647923	22	-	26627737	HeLa	viability after 18 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.2008772179449665	[673]	[81,259,284]	-2	hSpCas9	negative selection	1723387
39570956	39570979	22	+	26627737	HCT116	viability after 6 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	2.6692602571875668	[0]	[0,0]	9	hSpCas9	negative selection	1805697
39598181	39598204	22	-	26627737	HCT116	viability after 6 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	-0.4752042205027813	[332]	[85,10]	-3	hSpCas9	negative selection	1805698
39619397	39619420	22	-	26627737	HCT116	viability after 6 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.2494237909779566	[616]	[189,252]	6	hSpCas9	negative selection	1805699
39640977	39641000	22	-	26627737	HCT116	viability after 6 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.14979985782132488	[1590]	[174,333]	0	hSpCas9	negative selection	1805700
39646592	39646615	22	-	26627737	HCT116	viability after 6 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	2.432000585604784	[678]	[100,837]	9	hSpCas9	negative selection	1805701
39647900	39647923	22	-	26627737	HCT116	viability after 6 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.014222758341687958	[782]	[113,129]	0	hSpCas9	negative selection	1805702
39570956	39570979	22	+	26627737	HCT116	viability after 8 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8598070938499998	[0]	[0,0,0]	7	hSpCas9	negative selection	1888012
39598181	39598204	22	-	26627737	HCT116	viability after 8 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.5667940309721657	[144]	[95,86,170]	5	hSpCas9	negative selection	1888013
39619397	39619420	22	-	26627737	HCT116	viability after 8 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.7440592703270346	[121]	[157,260,254]	9	hSpCas9	negative selection	1888014
39640977	39641000	22	-	26627737	HCT116	viability after 8 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.15986550122431709	[763]	[435,356,338]	-2	hSpCas9	negative selection	1888015
39646592	39646615	22	-	26627737	HCT116	viability after 8 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.25315275018410455	[361]	[188,187,125]	-3	hSpCas9	negative selection	1888016
39647900	39647923	22	-	26627737	HCT116	viability after 8 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.019565095467161286	[389]	[208,197,245]	0	hSpCas9	negative selection	1888017
39570956	39570979	22	+	26627737	HCT116	viability after 9 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.9049898113534676	[0]	[0,0]	9	hSpCas9	negative selection	1970327
39598181	39598204	22	-	26627737	HCT116	viability after 9 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.20578878882878382	[332]	[144,68]	1	hSpCas9	negative selection	1970328
39619397	39619420	22	-	26627737	HCT116	viability after 9 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.39409577052616496	[616]	[97,146]	-3	hSpCas9	negative selection	1970329
39640977	39641000	22	-	26627737	HCT116	viability after 9 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.5529649257726479	[1590]	[305,873]	4	hSpCas9	negative selection	1970330
39646592	39646615	22	-	26627737	HCT116	viability after 9 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.8996877506689798	[678]	[166,41]	-6	hSpCas9	negative selection	1970331
39647900	39647923	22	-	26627737	HCT116	viability after 9 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.9546769973848759	[782]	[27,170]	-6	hSpCas9	negative selection	1970332
39570956	39570979	22	+	26627737	HCT116	viability after 12 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.9281650954308303	[0]	[0,0,0]	7	hSpCas9	negative selection	2052642
39598181	39598204	22	-	26627737	HCT116	viability after 12 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.8791194456539854	[144]	[55,269,104]	7	hSpCas9	negative selection	2052643
39619397	39619420	22	-	26627737	HCT116	viability after 12 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	2.1714341874997234	[121]	[296,299,268]	9	hSpCas9	negative selection	2052644
39640977	39641000	22	-	26627737	HCT116	viability after 12 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.08972753536152167	[763]	[316,493,328]	-1	hSpCas9	negative selection	2052645
39646592	39646615	22	-	26627737	HCT116	viability after 12 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.17235876632888975	[361]	[203,211,227]	1	hSpCas9	negative selection	2052646
39647900	39647923	22	-	26627737	HCT116	viability after 12 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.15567704646814604	[389]	[147,95,308]	-2	hSpCas9	negative selection	2052647
39570956	39570979	22	+	26627737	HCT116	viability after 15 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8001368291432343	[0]	[0,0,0]	7	hSpCas9	negative selection	2134957
39598181	39598204	22	-	26627737	HCT116	viability after 15 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.6276635375874966	[144]	[69,192,115]	6	hSpCas9	negative selection	2134958
39619397	39619420	22	-	26627737	HCT116	viability after 15 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	1.3308602551782893	[121]	[256,223,58]	8	hSpCas9	negative selection	2134959
39640977	39641000	22	-	26627737	HCT116	viability after 15 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.05036146860661539	[763]	[616,490,276]	0	hSpCas9	negative selection	2134960
39646592	39646615	22	-	26627737	HCT116	viability after 15 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.023061541620062354	[361]	[256,145,235]	0	hSpCas9	negative selection	2134961
39647900	39647923	22	-	26627737	HCT116	viability after 15 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.06713352673064338	[389]	[192,244,200]	0	hSpCas9	negative selection	2134962
39570956	39570979	22	+	26627737	HCT116	viability after 18 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	1.0485523756554223	[0]	[0,0,0]	7	hSpCas9	negative selection	2217272
39598181	39598204	22	-	26627737	HCT116	viability after 18 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	1.6237077239843576	[144]	[98,435,92]	9	hSpCas9	negative selection	2217273
39619397	39619420	22	-	26627737	HCT116	viability after 18 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	2.3200871701249826	[121]	[226,352,299]	9	hSpCas9	negative selection	2217274
39640977	39641000	22	-	26627737	HCT116	viability after 18 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.27350889181997573	[763]	[383,347,175]	-3	hSpCas9	negative selection	2217275
39646592	39646615	22	-	26627737	HCT116	viability after 18 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.21033989824906124	[361]	[208,194,203]	1	hSpCas9	negative selection	2217276
39647900	39647923	22	-	26627737	HCT116	viability after 18 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.6691720614061397	[389]	[88,93,176]	-6	hSpCas9	negative selection	2217277
39576343	39576366	22	-	25494202	A375	resistance to PLX-4720 (puromycin)	CACTTTCCTCATCTGTAAAATGG	CACNA1I	ENSG00000100346	0.07375301579419674	[56,81]	[7,23]	0	dCas9-VP64	positive selection	2386801
39625181	39625204	22	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAAGCGTGAGCCACCGTGCCCAG	CACNA1I	ENSG00000100346	1.3051580023585738	[14,7]	[5,4]	5	dCas9-VP64	positive selection	2476715
39576343	39576366	22	-	25494202	A375	resistance to PLX-4720 (zeocin)	CACTTTCCTCATCTGTAAAATGG	CACNA1I	ENSG00000100346	-0.8821779957181084	[358,162]	[56,35]	-8	dCas9-VP64	positive selection	2482056
39625181	39625204	22	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAAGCGTGAGCCACCGTGCCCAG	CACNA1I	ENSG00000100346	1.1554864993418033	[20,247]	[176,21]	7	dCas9-VP64	positive selection	2571970
39662396	39662419	22	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	-0.19238004316493373	[3,3]	[2,2]	-3	hSpCas9	positive selection	2975898
39600578	39600601	22	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	0.3101024282372406	[4,4]	[4,5]	6	hSpCas9	positive selection	2975899
39598244	39598267	22	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	-0.3923565708609598	[2,3]	[3,0]	-5	hSpCas9	positive selection	2975900
39600624	39600647	22	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	0.2992977505174336	[3,3]	[3,3]	6	hSpCas9	positive selection	2975901
39662396	39662419	22	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	-0.02099944011916073	[3,3]	[3,1]	0	hSpCas9	positive selection	3043756
39600578	39600601	22	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	0.46244467005626966	[4,4]	[4,4]	7	hSpCas9	positive selection	3043757
39598244	39598267	22	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	-0.38241049718355946	[2,2]	[0,1]	-5	hSpCas9	positive selection	3043758
39600624	39600647	22	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	0.5178506503057317	[3,3]	[2,4]	8	hSpCas9	positive selection	3043759
39570956	39570979		-	24336571	A375	resistance to PLX after 7 days	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.10424472732244539	[50,56]	[54,60]	3	hSpCas9	positive selection	3160192
39600627	39600650		-	24336571	A375	resistance to PLX after 7 days	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.03960160761591719	[18,15]	[18,16]	1	hSpCas9	positive selection	3160193
39570817	39570840		-	24336571	A375	resistance to PLX after 7 days	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	-0.1615626145136515	[17,17]	[16,15]	-5	hSpCas9	positive selection	3160194
39570956	39570979		-	24336571	A375	resistance to PLX after 14 days	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	-1.0281053083247638	[65,47]	[12,11]	-9	hSpCas9	positive selection	3217985
39600627	39600650		-	24336571	A375	resistance to PLX after 14 days	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.2722895846912619	[20,19]	[7,6]	-3	hSpCas9	positive selection	3217986
39570817	39570840		-	24336571	A375	resistance to PLX after 14 days	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	-0.7783671107653752	[17,22]	[4,4]	-8	hSpCas9	positive selection	3217987
39570956	39570979		-	24336571	A375	viability after 7 days	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.5229310202966511	[38]	[50,56]	7	hSpCas9	negative selection	3275778
39600627	39600650		-	24336571	A375	viability after 7 days	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.10233354973473174	[19]	[18,15]	-1	hSpCas9	negative selection	3275779
39570817	39570840		-	24336571	A375	viability after 7 days	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	0.08223791436083638	[17]	[17,17]	1	hSpCas9	negative selection	3275780
39570956	39570979		-	24336571	A375	viability after 14 days	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.612611130505434	[38]	[65,47]	7	hSpCas9	negative selection	3333571
39600627	39600650		-	24336571	A375	viability after 14 days	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.12472885729677063	[19]	[20,19]	1	hSpCas9	negative selection	3333572
39570817	39570840		-	24336571	A375	viability after 14 days	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	0.2550831044514118	[17]	[17,22]	3	hSpCas9	negative selection	3333573
39619357	39619380	22	+	27383988	293T	resistance to West Nile virus (flavivirus)	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	2.0098950665056456	[35,39]	[0,0]	7	hSpCas9	positive selection	3340734
39619363	39619386	22	-	27383988	293T	resistance to West Nile virus (flavivirus)	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	1.0939642958416878	[97,44]	[0,0]	5	hSpCas9	positive selection	3340735
39600589	39600612	22	+	27383988	293T	resistance to West Nile virus (flavivirus)	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.0005547997320064211	[142,162]	[0,0]	0	hSpCas9	positive selection	3340736
39570956	39570979	22	-	27383988	293T	resistance to West Nile virus (flavivirus)	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	-1.90506511731107	[572,572]	[0,0]	-9	hSpCas9	positive selection	3359988
39570794	39570817	22	-	27383988	293T	resistance to West Nile virus (flavivirus)	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.7654731151161606	[89,89]	[0,0]	4	hSpCas9	positive selection	3359989
39600627	39600650	22	-	27383988	293T	resistance to West Nile virus (flavivirus)	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-1.7952418393583185	[530,530]	[0,0]	-9	hSpCas9	positive selection	3359990
39662396	39662419	22	-	26780180	HT29	viability (Avana library 4 designs)	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	1.60868024461562	[]	[]	6	hSpCas9	negative selection	3412173
39600578	39600601	22	-	26780180	HT29	viability (Avana library 4 designs)	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	0.603740004417992	[]	[]	2	hSpCas9	negative selection	3412174
39598244	39598267	22	-	26780180	HT29	viability (Avana library 4 designs)	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	0.892515684966489	[]	[]	3	hSpCas9	negative selection	3412175
39600624	39600647	22	-	26780180	HT29	viability (Avana library 4 designs)	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	1.95264499102616	[]	[]	7	hSpCas9	negative selection	3412176
39600624	39600647	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	-7.30538328243838	[]	[]	-6	hSpCas9	negative selection	3487505
39662396	39662419	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	4.70448680378842	[]	[]	7	hSpCas9	negative selection	3487506
39600578	39600601	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	-0.625116223203409	[]	[]	-1	hSpCas9	negative selection	3487507
39598244	39598267	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	2.82823614986566	[]	[]	4	hSpCas9	negative selection	3487508
39662326	39662349	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	ATTGCAGTCCTCATGCCCGGGGG	CACNA1I	ENSG00000100346	0.852065661756236	[]	[]	1	hSpCas9	negative selection	3487509
39634695	39634718	22	-	26780180	A375	viability (Avana lentiCRISPRv2)	AGTTCTCCTCCAGGAAGCAGCGG	CACNA1I	ENSG00000100346	3.55696975387344	[]	[]	5	hSpCas9	negative selection	3487510
39600624	39600647	22	-	26780180	A375	viability (Avana lentiGuide)	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	-2.75996035148878	[]	[]	-5	hSpCas9	negative selection	3596164
39662396	39662419	22	-	26780180	A375	viability (Avana lentiGuide)	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	-3.54727449943382	[]	[]	-6	hSpCas9	negative selection	3596165
39600578	39600601	22	-	26780180	A375	viability (Avana lentiGuide)	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	-2.74798783400242	[]	[]	-5	hSpCas9	negative selection	3596166
39598244	39598267	22	-	26780180	A375	viability (Avana lentiGuide)	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	-0.186237072767005	[]	[]	0	hSpCas9	negative selection	3596167
39662326	39662349	22	-	26780180	A375	viability (Avana lentiGuide)	ATTGCAGTCCTCATGCCCGGGGG	CACNA1I	ENSG00000100346	8.9102902497645	[]	[]	6	hSpCas9	negative selection	3596168
39634695	39634718	22	-	26780180	A375	viability (Avana lentiGuide)	AGTTCTCCTCCAGGAAGCAGCGG	CACNA1I	ENSG00000100346	3.95899962640664	[]	[]	4	hSpCas9	negative selection	3596169
39600624	39600647	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	-0.1489735262472157	[4]	[4,3]	-2	hSpCas9	positive selection	3704823
39662396	39662419	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	0.09834753758502872	[4]	[5,3]	2	hSpCas9	positive selection	3704824
39600578	39600601	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	-0.2817694713419333	[5]	[5,3]	-4	hSpCas9	positive selection	3704825
39598244	39598267	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	0.2835724063517705	[4]	[6,5]	7	hSpCas9	positive selection	3704826
39662326	39662349	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ATTGCAGTCCTCATGCCCGGGGG	CACNA1I	ENSG00000100346	0.1535121102304208	[5]	[5,5]	4	hSpCas9	positive selection	3704827
39634695	39634718	22	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGTTCTCCTCCAGGAAGCAGCGG	CACNA1I	ENSG00000100346	0.15989104063710577	[4]	[4,5]	4	hSpCas9	positive selection	3704828
39600624	39600647	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGACGATGAAGAAATCCAGGCGG	CACNA1I	ENSG00000100346	-0.095013325905238	[5]	[4,3]	-1	hSpCas9	positive selection	3813430
39662396	39662419	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CATCCTCCCCGCGATCCCCGCGG	CACNA1I	ENSG00000100346	0.17899966618087157	[5]	[6,4]	4	hSpCas9	positive selection	3813431
39600578	39600601	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CTTCTTGCCAAAAATCCCCAGGG	CACNA1I	ENSG00000100346	0.20363146138001006	[6]	[6,5]	5	hSpCas9	positive selection	3813432
39598244	39598267	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TCACCTGCAGGATCTTGCAGCGG	CACNA1I	ENSG00000100346	0.1844212929431582	[4]	[4,4]	4	hSpCas9	positive selection	3813433
39662326	39662349	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ATTGCAGTCCTCATGCCCGGGGG	CACNA1I	ENSG00000100346	-0.38855754155870476	[5]	[2,3]	-5	hSpCas9	positive selection	3813434
39634695	39634718	22	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGTTCTCCTCCAGGAAGCAGCGG	CACNA1I	ENSG00000100346	-0.4468475443446197	[5]	[1,3]	-5	hSpCas9	positive selection	3813435
39570956	39570979	22	-	26780180	A375	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.102170965444688	[]	[]	0	hSpCas9	negative selection	3921730
39600627	39600650	22	-	26780180	A375	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	1.03621129406447	[]	[]	5	hSpCas9	negative selection	3921731
39619363	39619386	22	-	26780180	A375	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.657317268050966	[]	[]	2	hSpCas9	negative selection	3921732
39619357	39619380	22	+	26780180	A375	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.819909876817445	[]	[]	3	hSpCas9	negative selection	3921733
39570794	39570817	22	-	26780180	A375	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	1.25953975015256	[]	[]	6	hSpCas9	negative selection	3921734
39600589	39600612	22	+	26780180	A375	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.24428659218623	[]	[]	0	hSpCas9	negative selection	3921735
39570956	39570979	22	-	26780180	HT29	viability (GeCKOv2 library)	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	-0.0450428137563775	[]	[]	-1	hSpCas9	negative selection	4029696
39600627	39600650	22	-	26780180	HT29	viability (GeCKOv2 library)	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	2.03725716185025	[]	[]	9	hSpCas9	negative selection	4029697
39619363	39619386	22	-	26780180	HT29	viability (GeCKOv2 library)	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	1.42916520137682	[]	[]	7	hSpCas9	negative selection	4029698
39619357	39619380	22	+	26780180	HT29	viability (GeCKOv2 library)	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	1.27427807294037	[]	[]	6	hSpCas9	negative selection	4029699
39570794	39570817	22	-	26780180	HT29	viability (GeCKOv2 library)	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.875535171774894	[]	[]	3	hSpCas9	negative selection	4029700
39600589	39600612	22	+	26780180	HT29	viability (GeCKOv2 library)	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.325768726217058	[]	[]	0	hSpCas9	negative selection	4029701
39570956	39570979	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.07382750701312668	[5]	[3,3]	2	hSpCas9	positive selection	4188260
39600627	39600650	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.09770755211462331	[3]	[2,2]	2	hSpCas9	positive selection	4188261
39570817	39570840	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	-0.21857910887388726	[5]	[3,2]	-5	hSpCas9	positive selection	4188262
39570956	39570979	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	1.3726281129811106	[5]	[5,6,5,5]	9	hSpCas9	positive selection	4252282
39600627	39600650	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.17623375897937466	[4]	[0,3,2,0]	-3	hSpCas9	positive selection	4252283
39570817	39570840	22	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCGGGGTCCGGGCTGCTCCGTGG	CACNA1I	ENSG00000100346	1.0861683055421798	[4]	[2,3,3,4]	7	hSpCas9	positive selection	4252284
39619357	39619380	22	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.3150319863975383	[1]	[2,1]	6	hSpCas9	positive selection	4260599
39619363	39619386	22	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.1718198698822612	[2]	[2,1]	3	hSpCas9	positive selection	4260600
39600589	39600612	22	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.3309328225585476	[3]	[3,3]	6	hSpCas9	positive selection	4260601
39570956	39570979	22	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.1995725823256813	[4]	[4,3]	4	hSpCas9	positive selection	4324880
39570794	39570817	22	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.026919348492625506	[2]	[2,0]	0	hSpCas9	positive selection	4324881
39600627	39600650	22	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.36855357452558535	[4]	[3,0]	-7	hSpCas9	positive selection	4324882
39660351	39660373	22	-	27760321	OCIAML3	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	0.12151181165803565	[586,510]	[422,521]	1	hSpCas9	negative selection	4385123
39662043	39662065	22	+	27760321	OCIAML3	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.6470596519372295	[911,710]	[782,1252]	7	hSpCas9	negative selection	4385124
39662117	39662139	22	+	27760321	OCIAML3	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.7247138373082662	[785,671]	[874,1022]	8	hSpCas9	negative selection	4385125
39662382	39662404	22	+	27760321	OCIAML3	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.3269431506301014	[544,422]	[457,488]	4	hSpCas9	negative selection	4385126
39641022	39641044	22	+	27760321	OCIAML3	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	0.4042084329393233	[955,794]	[656,1221]	5	hSpCas9	negative selection	4385127
39660351	39660373	22	-	27760321	OCIAML2	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.0008572172289662006	[586,510]	[368,539]	0	hSpCas9	negative selection	4468586
39662043	39662065	22	+	27760321	OCIAML2	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.4013231760621404	[911,710]	[728,1034]	5	hSpCas9	negative selection	4468587
39662117	39662139	22	+	27760321	OCIAML2	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.3347943890069094	[785,671]	[718,770]	4	hSpCas9	negative selection	4468588
39662382	39662404	22	+	27760321	OCIAML2	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.2981322422113989	[544,422]	[433,535]	4	hSpCas9	negative selection	4468589
39641022	39641044	22	+	27760321	OCIAML2	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.21727956837414564	[955,794]	[452,808]	-3	hSpCas9	negative selection	4468590
39660351	39660373	22	-	27760321	MV411	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.21189577600424248	[586,510]	[369,224]	-1	hSpCas9	negative selection	4552049
39662043	39662065	22	+	27760321	MV411	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.22242188655607642	[911,710]	[709,485]	1	hSpCas9	negative selection	4552050
39662117	39662139	22	+	27760321	MV411	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.6971636823895704	[785,671]	[677,926]	5	hSpCas9	negative selection	4552051
39662382	39662404	22	+	27760321	MV411	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.532560595512837	[544,422]	[140,935]	4	hSpCas9	negative selection	4552052
39641022	39641044	22	+	27760321	MV411	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.2654278961086076	[955,794]	[243,830]	-2	hSpCas9	negative selection	4552053
39660351	39660373	22	-	27760321	HL60	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	0.16053862849633266	[586,510]	[567,427]	2	hSpCas9	negative selection	4635512
39662043	39662065	22	+	27760321	HL60	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.43530435671252776	[911,710]	[1078,689]	5	hSpCas9	negative selection	4635513
39662117	39662139	22	+	27760321	HL60	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.23741122876402498	[785,671]	[693,705]	3	hSpCas9	negative selection	4635514
39662382	39662404	22	+	27760321	HL60	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	1.2625793349880732	[544,422]	[672,1226]	9	hSpCas9	negative selection	4635515
39641022	39641044	22	+	27760321	HL60	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	0.5740572088316306	[955,794]	[1064,1054]	6	hSpCas9	negative selection	4635516
39660351	39660373	22	-	27760321	HT1080	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	1.8061941227207563	[510,398]	[610,658]	8	hSpCas9	negative selection	4718975
39662043	39662065	22	+	27760321	HT1080	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.7532254254714136	[710,554]	[404,446]	4	hSpCas9	negative selection	4718976
39662117	39662139	22	+	27760321	HT1080	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	1.774587677394862	[671,524]	[937,681]	8	hSpCas9	negative selection	4718977
39662382	39662404	22	+	27760321	HT1080	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.7948172371998309	[422,329]	[158,370]	4	hSpCas9	negative selection	4718978
39641022	39641044	22	+	27760321	HT1080	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	0.545451589352668	[794,620]	[393,430]	3	hSpCas9	negative selection	4718979
39660351	39660373	22	-	27760321	HT29	viability after 7 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.3576590339671996	[510,398]	[553,501,330]	-5	hSpCas9	negative selection	4802438
39662043	39662065	22	+	27760321	HT29	viability after 7 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.38294630923491846	[710,554]	[1286,818,1106]	6	hSpCas9	negative selection	4802439
39662117	39662139	22	+	27760321	HT29	viability after 7 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.2524930001828589	[671,524]	[1295,877,637]	4	hSpCas9	negative selection	4802440
39662382	39662404	22	+	27760321	HT29	viability after 7 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.2160683979648394	[422,329]	[782,484,452]	3	hSpCas9	negative selection	4802441
39641022	39641044	22	+	27760321	HT29	viability after 7 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.02172798324712999	[794,620]	[698,788,1163]	0	hSpCas9	negative selection	4802442
39660351	39660373	22	-	27760321	HT29	viability after 25 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	0.1570098142276719	[510,398]	[679,559,481]	1	hSpCas9	negative selection	4885901
39662043	39662065	22	+	27760321	HT29	viability after 25 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.5534614111456314	[710,554]	[833,1239,1130]	6	hSpCas9	negative selection	4885902
39662117	39662139	22	+	27760321	HT29	viability after 25 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.5943748363506706	[671,524]	[1039,990,1068]	6	hSpCas9	negative selection	4885903
39662382	39662404	22	+	27760321	HT29	viability after 25 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.767864385261807	[422,329]	[942,408,842]	8	hSpCas9	negative selection	4885904
39641022	39641044	22	+	27760321	HT29	viability after 25 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	0.009388098268358291	[794,620]	[614,849,1009]	0	hSpCas9	negative selection	4885905
39660351	39660373	22	-	27760321	HT29	viability after 22 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	0.11672388575371695	[510,398]	[654,558,493]	1	hSpCas9	negative selection	4969364
39662043	39662065	22	+	27760321	HT29	viability after 22 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.7448356441700269	[710,554]	[977,1544,1161]	8	hSpCas9	negative selection	4969365
39662117	39662139	22	+	27760321	HT29	viability after 22 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.4579220367260243	[671,524]	[879,826,1147]	5	hSpCas9	negative selection	4969366
39662382	39662404	22	+	27760321	HT29	viability after 22 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.86149464980295	[422,329]	[722,671,980]	8	hSpCas9	negative selection	4969367
39641022	39641044	22	+	27760321	HT29	viability after 22 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.5714426779226376	[794,620]	[328,719,609]	-5	hSpCas9	negative selection	4969368
39660351	39660373	22	-	27760321	HT29	viability after 19 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.07840489567001863	[510,398]	[595,666,235]	0	hSpCas9	negative selection	5052827
39662043	39662065	22	+	27760321	HT29	viability after 19 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.8662150143578699	[710,554]	[1144,1371,1418]	8	hSpCas9	negative selection	5052828
39662117	39662139	22	+	27760321	HT29	viability after 19 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.5491022801619004	[671,524]	[597,1017,1332]	5	hSpCas9	negative selection	5052829
39662382	39662404	22	+	27760321	HT29	viability after 19 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.5097755353259933	[422,329]	[725,574,551]	5	hSpCas9	negative selection	5052830
39641022	39641044	22	+	27760321	HT29	viability after 19 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.4514540632277131	[794,620]	[775,630,395]	-4	hSpCas9	negative selection	5052831
39660351	39660373	22	-	27760321	HT29	viability after 16 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.26571943990033486	[510,398]	[530,323,427]	-3	hSpCas9	negative selection	5136290
39662043	39662065	22	+	27760321	HT29	viability after 16 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.4083846819442202	[710,554]	[1281,823,748]	4	hSpCas9	negative selection	5136291
39662117	39662139	22	+	27760321	HT29	viability after 16 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.6859488711624439	[671,524]	[1099,1148,982]	7	hSpCas9	negative selection	5136292
39662382	39662404	22	+	27760321	HT29	viability after 16 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.8220005312141399	[422,329]	[894,554,802]	8	hSpCas9	negative selection	5136293
39641022	39641044	22	+	27760321	HT29	viability after 16 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.16160074773421507	[794,620]	[577,869,659]	-2	hSpCas9	negative selection	5136294
39660351	39660373	22	-	27760321	HT29	viability after 13 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	0.011426177139394533	[510,398]	[874,370,359]	0	hSpCas9	negative selection	5219753
39662043	39662065	22	+	27760321	HT29	viability after 13 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.6020896464822519	[710,554]	[909,1296,1135]	7	hSpCas9	negative selection	5219754
39662117	39662139	22	+	27760321	HT29	viability after 13 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.5370526897800304	[671,524]	[1097,1117,817]	7	hSpCas9	negative selection	5219755
39662382	39662404	22	+	27760321	HT29	viability after 13 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.31544054201694105	[422,329]	[545,669,421]	4	hSpCas9	negative selection	5219756
39641022	39641044	22	+	27760321	HT29	viability after 13 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.364467517219083	[794,620]	[638,756,524]	-4	hSpCas9	negative selection	5219757
39660351	39660373	22	-	27760321	HT29	viability after 10 days	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.18982779434765618	[510,398]	[579,438,473]	-2	hSpCas9	negative selection	5303216
39662043	39662065	22	+	27760321	HT29	viability after 10 days	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.6324338068949594	[710,554]	[1121,1264,1287]	8	hSpCas9	negative selection	5303217
39662117	39662139	22	+	27760321	HT29	viability after 10 days	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.2905986887786983	[671,524]	[1067,743,924]	4	hSpCas9	negative selection	5303218
39662382	39662404	22	+	27760321	HT29	viability after 10 days	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	0.5111396724294329	[422,329]	[846,556,603]	7	hSpCas9	negative selection	5303219
39641022	39641044	22	+	27760321	HT29	viability after 10 days	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	-0.31052471604818543	[794,620]	[664,866,612]	-4	hSpCas9	negative selection	5303220
39660351	39660373	22	-	27760321	MOLM13	viability	TCGTCCGAGTAGGAGCGATTGG	CACNA1I	ENSG00000100346	-0.6762922100918702	[586,510]	[248,145]	-4	hSpCas9	negative selection	5386679
39662043	39662065	22	+	27760321	MOLM13	viability	CTCAAGCACAAGCCGCCGTCGG	CACNA1I	ENSG00000100346	0.8482666298648572	[911,710]	[1287,418]	6	hSpCas9	negative selection	5386680
39662117	39662139	22	+	27760321	MOLM13	viability	CGAGGTTGCCGCGGACGAGGGG	CACNA1I	ENSG00000100346	0.8343001371488861	[785,671]	[790,679]	6	hSpCas9	negative selection	5386681
39662382	39662404	22	+	27760321	MOLM13	viability	ACCAAGATGGGCGACCGCGGGG	CACNA1I	ENSG00000100346	1.8545437707751786	[544,422]	[609,1295]	9	hSpCas9	negative selection	5386682
39641022	39641044	22	+	27760321	MOLM13	viability	ACGTCTACGACTTTGGGGCGGG	CACNA1I	ENSG00000100346	0.2677713448747773	[955,794]	[737,467]	1	hSpCas9	negative selection	5386683
39571119	39571142	22	+	27661255	K562	viability	GTGGTGGTGAGCGAGCTCAGAGG	CACNA1I	ENSG00000100346	0.52338408786544	[638,692]	[904,642]	9	dCas9-KRAB	negative selection	5467696
39570774	39570797	22	-	27661255	K562	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	0.1438063882144176	[1553,1419]	[1436,1209]	5	dCas9-KRAB	negative selection	5467697
39570807	39570830	22	-	27661255	K562	viability	GGCTGCTCCGTGGTGACTCCTGG	CACNA1I	ENSG00000100346	0.10676596729966784	[651,818]	[777,503]	4	dCas9-KRAB	negative selection	5467698
39619357	39619380	22	+	27260156	BXPC3	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.04315649279138056	[128]	[144,112,143,155]	0	hSpCas9	negative selection	5533059
39619363	39619386	22	-	27260156	BXPC3	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.18304795745267388	[261]	[289,263,359,336]	4	hSpCas9	negative selection	5533060
39600589	39600612	22	+	27260156	BXPC3	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.1743877554581037	[666]	[697,706,738,1043]	4	hSpCas9	negative selection	5533061
39570956	39570979	22	-	27260156	BXPC3	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.22959379120842338	[1292]	[1501,1444,1611,1816]	5	hSpCas9	negative selection	5597340
39570794	39570817	22	-	27260156	BXPC3	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.17714074522468293	[252]	[238,164,242,298]	-4	hSpCas9	negative selection	5597341
39600627	39600650	22	-	27260156	BXPC3	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.0949716365467339	[1016]	[906,902,965,1244]	-2	hSpCas9	negative selection	5597342
39619357	39619380	22	+	27260156	A673	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.1432645147961129	[128]	[158,149,140,110]	-4	hSpCas9	negative selection	5654310
39619363	39619386	22	-	27260156	A673	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.06227341287627841	[261]	[372,317,326,298]	1	hSpCas9	negative selection	5654311
39600589	39600612	22	+	27260156	A673	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.09519882206027985	[666]	[935,759,849,887]	2	hSpCas9	negative selection	5654312
39570956	39570979	22	-	27260156	A673	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.11994765773332783	[1292]	[2044,1478,1550,1719]	3	hSpCas9	negative selection	5718591
39570794	39570817	22	-	27260156	A673	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.04725270794861913	[252]	[390,205,359,308]	1	hSpCas9	negative selection	5718592
39600627	39600650	22	-	27260156	A673	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.05749337648395958	[1016]	[1200,1226,1090,1174]	-1	hSpCas9	negative selection	5718593
39619357	39619380	22	+	27260156	A375	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.14055847444067315	[128]	[138,129,99,171]	-2	hSpCas9	negative selection	5775561
39619363	39619386	22	-	27260156	A375	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.2568973907941978	[261]	[151,357,133,377]	-4	hSpCas9	negative selection	5775562
39600589	39600612	22	+	27260156	A375	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.3105987950041271	[666]	[1061,1341,765,767]	4	hSpCas9	negative selection	5775563
39570956	39570979	22	-	27260156	A375	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.4576814954353954	[1292]	[1806,2917,1794,1903]	6	hSpCas9	negative selection	5839842
39570794	39570817	22	-	27260156	A375	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.16261473844256935	[252]	[233,456,231,169]	-2	hSpCas9	negative selection	5839843
39600627	39600650	22	-	27260156	A375	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.22246412048126585	[1016]	[1403,1829,1210,1179]	3	hSpCas9	negative selection	5839844
39619357	39619380	22	+	27260156	COLO741	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.317464352344037	[128]	[172,165,65]	-6	hSpCas9	negative selection	5896812
39619363	39619386	22	-	27260156	COLO741	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.28360270752495065	[261]	[439,331,448]	6	hSpCas9	negative selection	5896813
39600589	39600612	22	+	27260156	COLO741	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.2717373864460939	[666]	[1201,1137,777]	6	hSpCas9	negative selection	5896814
39570956	39570979	22	-	27260156	COLO741	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.357916128271732	[1292]	[2190,1917,2234]	7	hSpCas9	negative selection	5961093
39570794	39570817	22	-	27260156	COLO741	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.3131819766120387	[252]	[318,235,231]	-6	hSpCas9	negative selection	5961094
39600627	39600650	22	-	27260156	COLO741	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.09077706140483599	[1016]	[1331,1315,1487]	2	hSpCas9	negative selection	5961095
39619357	39619380	22	+	27260156	CAL120	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.12440311245757396	[128]	[153,43,242]	-2	hSpCas9	negative selection	6018063
39619363	39619386	22	-	27260156	CAL120	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.322532834313906	[261]	[289,266,229]	-5	hSpCas9	negative selection	6018064
39600589	39600612	22	+	27260156	CAL120	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.5568097105779598	[666]	[1393,1219,1076]	8	hSpCas9	negative selection	6018065
39570956	39570979	22	-	27260156	CAL120	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.04430176745122205	[1292]	[1525,1839,1591]	0	hSpCas9	negative selection	6082344
39570794	39570817	22	-	27260156	CAL120	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.45168619466674054	[252]	[144,375,157]	-6	hSpCas9	negative selection	6082345
39600627	39600650	22	-	27260156	CAL120	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.11968562166105068	[1016]	[1406,1499,1224]	1	hSpCas9	negative selection	6082346
39619357	39619380	22	+	27260156	CADOES1	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.24032193744984798	[128]	[149,173,99,142]	4	hSpCas9	negative selection	6139314
39619363	39619386	22	-	27260156	CADOES1	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.006967941719246529	[261]	[245,208,287,235]	0	hSpCas9	negative selection	6139315
39600589	39600612	22	+	27260156	CADOES1	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.15062756500300856	[666]	[751,611,679,710]	2	hSpCas9	negative selection	6139316
39570956	39570979	22	-	27260156	CADOES1	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.19987989793233019	[1292]	[1439,1246,1446,1390]	3	hSpCas9	negative selection	6203595
39570794	39570817	22	-	27260156	CADOES1	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.5292379005258938	[252]	[173,157,160,158]	-7	hSpCas9	negative selection	6203596
39600627	39600650	22	-	27260156	CADOES1	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.0356564228198768	[1016]	[851,1013,928,1087]	0	hSpCas9	negative selection	6203597
39619357	39619380	22	+	27260156	EWS502	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.04384498879203913	[128]	[128,108,142,187]	0	hSpCas9	negative selection	6260565
39619363	39619386	22	-	27260156	EWS502	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.16365569268255686	[261]	[229,332,377,310]	3	hSpCas9	negative selection	6260566
39600589	39600612	22	+	27260156	EWS502	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.23097550735967887	[666]	[831,783,779,924]	4	hSpCas9	negative selection	6260567
39570956	39570979	22	-	27260156	EWS502	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.18532765110968397	[1292]	[1244,1282,1791,1985]	3	hSpCas9	negative selection	6324846
39570794	39570817	22	-	27260156	EWS502	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.03570537032040144	[252]	[304,374,292,104]	0	hSpCas9	negative selection	6324847
39600627	39600650	22	-	27260156	EWS502	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.11054634610761882	[1016]	[1085,929,1246,1422]	2	hSpCas9	negative selection	6324848
39619357	39619380	22	+	27260156	EW8	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.1519508852134579	[128]	[141,86,127,107]	3	hSpCas9	negative selection	6381816
39619363	39619386	22	-	27260156	EW8	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.24567976753373078	[261]	[303,102,120,210]	-4	hSpCas9	negative selection	6381817
39600589	39600612	22	+	27260156	EW8	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.15499415343531697	[666]	[780,576,539,537]	3	hSpCas9	negative selection	6381818
39570956	39570979	22	-	27260156	EW8	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.1404728518444167	[1292]	[1282,1108,1045,1210]	2	hSpCas9	negative selection	6446097
39570794	39570817	22	-	27260156	EW8	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.47699302507480645	[252]	[217,144,85,157]	-7	hSpCas9	negative selection	6446098
39600627	39600650	22	-	27260156	EW8	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.08542957259396777	[1016]	[1093,935,649,877]	1	hSpCas9	negative selection	6446099
39619357	39619380	22	+	27260156	CORL105	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.1315041454488799	[128]	[169,165,178,148]	2	hSpCas9	negative selection	6503067
39619363	39619386	22	-	27260156	CORL105	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.5144291502818594	[261]	[551,219,449,539]	8	hSpCas9	negative selection	6503068
39600589	39600612	22	+	27260156	CORL105	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.3670107610939539	[666]	[967,833,1450,846]	6	hSpCas9	negative selection	6503069
39570956	39570979	22	-	27260156	CORL105	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.30685691448304664	[1292]	[2135,1822,1840,1694]	6	hSpCas9	negative selection	6567348
39570794	39570817	22	-	27260156	CORL105	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.003920100530574899	[252]	[359,219,294,309]	0	hSpCas9	negative selection	6567349
39600627	39600650	22	-	27260156	CORL105	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.20625364831691906	[1016]	[1385,1206,1730,1229]	4	hSpCas9	negative selection	6567350
39619357	39619380	22	+	27260156	HS294T	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	1.2131012833040393	[128]	[62,202,103,89]	9	hSpCas9	negative selection	6624318
39619363	39619386	22	-	27260156	HS294T	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.19384847024184892	[261]	[112,107,149,87]	2	hSpCas9	negative selection	6624319
39600589	39600612	22	+	27260156	HS294T	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.5683843503887142	[666]	[348,452,305,428]	7	hSpCas9	negative selection	6624320
39570956	39570979	22	-	27260156	HS294T	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.38086774877803187	[1292]	[590,822,494,700]	5	hSpCas9	negative selection	6688599
39570794	39570817	22	-	27260156	HS294T	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.17191390811736962	[252]	[66,132,82,170]	2	hSpCas9	negative selection	6688600
39600627	39600650	22	-	27260156	HS294T	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.09462013392633728	[1016]	[456,298,415,523]	1	hSpCas9	negative selection	6688601
39619357	39619380	22	+	27260156	HCC44	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.6933650465023156	[128]	[224,44,88,21]	-7	hSpCas9	negative selection	6745569
39619363	39619386	22	-	27260156	HCC44	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.266552455083948	[261]	[329,84,333,278]	-4	hSpCas9	negative selection	6745570
39600589	39600612	22	+	27260156	HCC44	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.3244418668276884	[666]	[954,950,1147,757]	5	hSpCas9	negative selection	6745571
39570956	39570979	22	-	27260156	HCC44	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.45762672214966793	[1292]	[2259,1626,1994,2202]	7	hSpCas9	negative selection	6809850
39570794	39570817	22	-	27260156	HCC44	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.19561717620979935	[252]	[266,218,586,290]	3	hSpCas9	negative selection	6809851
39600627	39600650	22	-	27260156	HCC44	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.17786287159089587	[1016]	[1357,1222,1344,1291]	3	hSpCas9	negative selection	6809852
39619357	39619380	22	+	27260156	G402	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.030142111705295793	[128]	[110,309,131,69]	0	hSpCas9	negative selection	6866820
39619363	39619386	22	-	27260156	G402	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.3707587562381185	[261]	[246,355,158,232]	-5	hSpCas9	negative selection	6866821
39600589	39600612	22	+	27260156	G402	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.4277378636028738	[666]	[1468,1212,855,906]	5	hSpCas9	negative selection	6866822
39570956	39570979	22	-	27260156	G402	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.16349209320043978	[1292]	[2170,1863,1444,1650]	2	hSpCas9	negative selection	6931101
39570794	39570817	22	-	27260156	G402	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.5607073295812832	[252]	[192,189,157,287]	-6	hSpCas9	negative selection	6931102
39600627	39600650	22	-	27260156	G402	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.22551738949867217	[1016]	[2058,1511,1198,1134]	3	hSpCas9	negative selection	6931103
39619357	39619380	22	+	27260156	L33	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.00031488573680782395	[128]	[152,29,89,145]	0	hSpCas9	negative selection	6988071
39619363	39619386	22	-	27260156	L33	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.2937885933114358	[261]	[344,112,204,336]	6	hSpCas9	negative selection	6988072
39600589	39600612	22	+	27260156	L33	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.2608373022869136	[666]	[763,388,421,811]	5	hSpCas9	negative selection	6988073
39570956	39570979	22	-	27260156	L33	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.14408114613525336	[1292]	[1444,606,899,1356]	3	hSpCas9	negative selection	7052352
39570794	39570817	22	-	27260156	L33	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.15497058158291677	[252]	[239,130,138,345]	3	hSpCas9	negative selection	7052353
39600627	39600650	22	-	27260156	L33	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.06641151457988137	[1016]	[1055,335,647,975]	-1	hSpCas9	negative selection	7052354
39619357	39619380	22	+	27260156	K562	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.129938205522788	[128]	[82,97,83,145]	-2	hSpCas9	negative selection	7109322
39619363	39619386	22	-	27260156	K562	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.05067682771795301	[261]	[287,201,280,139]	0	hSpCas9	negative selection	7109323
39600589	39600612	22	+	27260156	K562	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.30042460894543555	[666]	[916,1047,478,524]	4	hSpCas9	negative selection	7109324
39570956	39570979	22	-	27260156	K562	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.23541828342807647	[1292]	[1658,1074,1452,1234]	3	hSpCas9	negative selection	7173603
39570794	39570817	22	-	27260156	K562	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.08460180274666818	[252]	[282,416,142,39]	-1	hSpCas9	negative selection	7173604
39600627	39600650	22	-	27260156	K562	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.11389212477289745	[1016]	[1279,1132,814,735]	1	hSpCas9	negative selection	7173605
39619357	39619380	22	+	27260156	HT29	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.14306350403687595	[128]	[177,165,100,237]	2	hSpCas9	negative selection	7230573
39619363	39619386	22	-	27260156	HT29	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.14426681674125785	[261]	[398,456,245,319]	2	hSpCas9	negative selection	7230574
39600589	39600612	22	+	27260156	HT29	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.07253966655553068	[666]	[1006,785,805,815]	1	hSpCas9	negative selection	7230575
39570956	39570979	22	-	27260156	HT29	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.21943632247555112	[1292]	[1811,2168,1690,1680]	4	hSpCas9	negative selection	7294854
39570794	39570817	22	-	27260156	HT29	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.09765183646654774	[252]	[354,346,326,292]	1	hSpCas9	negative selection	7294855
39600627	39600650	22	-	27260156	HT29	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.026643204495059747	[1016]	[1410,1349,1053,1074]	0	hSpCas9	negative selection	7294856
39619357	39619380	22	+	27260156	MHHES1	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.09592903006099363	[128]	[157,148,129,112]	-2	hSpCas9	negative selection	7351824
39619363	39619386	22	-	27260156	MHHES1	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.08149012497603947	[261]	[592,357,109,235]	1	hSpCas9	negative selection	7351825
39600589	39600612	22	+	27260156	MHHES1	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.22766904153298795	[666]	[837,1093,921,702]	4	hSpCas9	negative selection	7351826
39570956	39570979	22	-	27260156	MHHES1	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.1034293888135549	[1292]	[1803,1469,1670,1347]	2	hSpCas9	negative selection	7416105
39570794	39570817	22	-	27260156	MHHES1	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.18088749610998123	[252]	[289,390,322,290]	3	hSpCas9	negative selection	7416106
39600627	39600650	22	-	27260156	MHHES1	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.1304329833170783	[1016]	[1277,1531,1178,1070]	2	hSpCas9	negative selection	7416107
39619357	39619380	22	+	27260156	MEWO	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.10103475949855834	[128]	[73,129,125]	-2	hSpCas9	negative selection	7473075
39619363	39619386	22	-	27260156	MEWO	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.1368825716911244	[261]	[276,254,277]	3	hSpCas9	negative selection	7473076
39600589	39600612	22	+	27260156	MEWO	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.20984040018728134	[666]	[836,787,561]	4	hSpCas9	negative selection	7473077
39570956	39570979	22	-	27260156	MEWO	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.08552082477365008	[1292]	[1711,1158,1062]	2	hSpCas9	negative selection	7537356
39570794	39570817	22	-	27260156	MEWO	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.1483147891866099	[252]	[199,221,215]	-3	hSpCas9	negative selection	7537357
39600627	39600650	22	-	27260156	MEWO	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.06947957630013968	[1016]	[1320,896,839]	1	hSpCas9	negative selection	7537358
39619357	39619380	22	+	27260156	LNCAPCLONEFGC	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.30419110340625033	[128]	[122,220,134,91]	5	hSpCas9	negative selection	7594326
39619363	39619386	22	-	27260156	LNCAPCLONEFGC	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.2668306353178037	[261]	[322,211,324,291]	4	hSpCas9	negative selection	7594327
39600589	39600612	22	+	27260156	LNCAPCLONEFGC	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.2528542387001824	[666]	[825,578,823,672]	4	hSpCas9	negative selection	7594328
39570956	39570979	22	-	27260156	LNCAPCLONEFGC	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.4736823168583677	[1292]	[1612,1533,2015,1405]	7	hSpCas9	negative selection	7658607
39570794	39570817	22	-	27260156	LNCAPCLONEFGC	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.23624837685902522	[252]	[367,197,151,346]	3	hSpCas9	negative selection	7658608
39600627	39600650	22	-	27260156	LNCAPCLONEFGC	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.36802318535346545	[1016]	[1394,1142,1309,923]	6	hSpCas9	negative selection	7658609
39619357	39619380	22	+	27260156	PANC0327	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.08323385567328634	[128]	[130,33,89,188]	-1	hSpCas9	negative selection	7715577
39619363	39619386	22	-	27260156	PANC0327	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.14641559171270713	[261]	[284,118,527,118]	2	hSpCas9	negative selection	7715578
39600589	39600612	22	+	27260156	PANC0327	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.4253546203391111	[666]	[1229,869,462,644]	6	hSpCas9	negative selection	7715579
39570956	39570979	22	-	27260156	PANC0327	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.26491270566960035	[1292]	[1326,1047,1719,1463]	4	hSpCas9	negative selection	7779858
39570794	39570817	22	-	27260156	PANC0327	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.2481759083674977	[252]	[138,179,242,191]	-4	hSpCas9	negative selection	7779859
39600627	39600650	22	-	27260156	PANC0327	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.1914848315748312	[1016]	[1098,982,1011,1043]	3	hSpCas9	negative selection	7779860
39619357	39619380	22	+	27260156	NCIH2009	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.25971088839122336	[128]	[177,243,216]	3	hSpCas9	negative selection	7836828
39619363	39619386	22	-	27260156	NCIH2009	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.2784267818873799	[261]	[421,489,413]	4	hSpCas9	negative selection	7836829
39600589	39600612	22	+	27260156	NCIH2009	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.6746137463592848	[666]	[1870,1195,1473]	8	hSpCas9	negative selection	7836830
39570956	39570979	22	-	27260156	NCIH2009	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.5382439663922698	[1292]	[3321,2399,2285]	7	hSpCas9	negative selection	7901109
39570794	39570817	22	-	27260156	NCIH2009	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.007699993093420843	[252]	[435,269,376]	0	hSpCas9	negative selection	7901110
39600627	39600650	22	-	27260156	NCIH2009	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.2607248662321924	[1016]	[1986,1341,1837]	3	hSpCas9	negative selection	7901111
39619357	39619380	22	+	27260156	NCIH1373	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.11110082884045647	[128]	[239,84,187,33]	1	hSpCas9	negative selection	7958079
39619363	39619386	22	-	27260156	NCIH1373	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.1676795484047955	[261]	[151,259,350,68]	-2	hSpCas9	negative selection	7958080
39600589	39600612	22	+	27260156	NCIH1373	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.5806026609060765	[666]	[1177,669,1607,276]	6	hSpCas9	negative selection	7958081
39570956	39570979	22	-	27260156	NCIH1373	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.5912940072572386	[1292]	[2619,2059,1457,712]	6	hSpCas9	negative selection	8022360
39570794	39570817	22	-	27260156	NCIH1373	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.3825949843527501	[252]	[175,376,119,44]	-4	hSpCas9	negative selection	8022361
39600627	39600650	22	-	27260156	NCIH1373	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.07302711070245627	[1016]	[1523,1195,1354,192]	0	hSpCas9	negative selection	8022362
39619357	39619380	22	+	27260156	PATU8902	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.9314900889255175	[128]	[158,496,413,273]	8	hSpCas9	negative selection	8079330
39619363	39619386	22	-	27260156	PATU8902	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.40767841970805807	[261]	[641,97,803,276]	4	hSpCas9	negative selection	8079331
39600589	39600612	22	+	27260156	PATU8902	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.5997151956513814	[666]	[1968,1425,1237,970]	6	hSpCas9	negative selection	8079332
39570956	39570979	22	-	27260156	PATU8902	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.466869579506459	[1292]	[1740,2194,2331,3883]	5	hSpCas9	negative selection	8143611
39570794	39570817	22	-	27260156	PATU8902	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.8206969288495112	[252]	[334,183,68,245]	-6	hSpCas9	negative selection	8143612
39600627	39600650	22	-	27260156	PATU8902	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.5536638816328747	[1016]	[2397,2043,1696,2278]	6	hSpCas9	negative selection	8143613
39619357	39619380	22	+	27260156	PANC1	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.2565582820580423	[128]	[133,84,220,85]	4	hSpCas9	negative selection	8200581
39619363	39619386	22	-	27260156	PANC1	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.29005433195549735	[261]	[189,494,325,152]	4	hSpCas9	negative selection	8200582
39600589	39600612	22	+	27260156	PANC1	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.36594904092765246	[666]	[777,1265,510,526]	6	hSpCas9	negative selection	8200583
39570956	39570979	22	-	27260156	PANC1	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.19924395935845984	[1292]	[1231,1655,1094,1187]	3	hSpCas9	negative selection	8264862
39570794	39570817	22	-	27260156	PANC1	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.16222408895155094	[252]	[243,259,271,202]	2	hSpCas9	negative selection	8264863
39600627	39600650	22	-	27260156	PANC1	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.05501029570834026	[1016]	[1126,1069,626,603]	-1	hSpCas9	negative selection	8264864
39619357	39619380	22	+	27260156	PANC0813	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.01075384488808151	[128]	[247,175,64,130]	0	hSpCas9	negative selection	8321832
39619363	39619386	22	-	27260156	PANC0813	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.08861706102438993	[261]	[535,170,229,386]	1	hSpCas9	negative selection	8321833
39600589	39600612	22	+	27260156	PANC0813	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.39090230422613537	[666]	[1059,977,981,1086]	6	hSpCas9	negative selection	8321834
39570956	39570979	22	-	27260156	PANC0813	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.3028575513639582	[1292]	[1937,1959,1923,1685]	5	hSpCas9	negative selection	8386113
39570794	39570817	22	-	27260156	PANC0813	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.41700486252332636	[252]	[467,450,388,290]	6	hSpCas9	negative selection	8386114
39600627	39600650	22	-	27260156	PANC0813	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.23408154642970258	[1016]	[1424,1173,1395,1612]	4	hSpCas9	negative selection	8386115
39619357	39619380	22	+	27260156	RDES	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.06285448978340225	[128]	[130,155,192,138]	1	hSpCas9	negative selection	8443083
39619363	39619386	22	-	27260156	RDES	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.3807927421278954	[261]	[208,153,303,272]	-6	hSpCas9	negative selection	8443084
39600589	39600612	22	+	27260156	RDES	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.09812063341227806	[666]	[707,696,785,1153]	1	hSpCas9	negative selection	8443085
39570956	39570979	22	-	27260156	RDES	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.316496127392635	[1292]	[1575,1830,1860,2194]	5	hSpCas9	negative selection	8507364
39570794	39570817	22	-	27260156	RDES	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.27529213603473823	[252]	[164,277,219,314]	-5	hSpCas9	negative selection	8507365
39600627	39600650	22	-	27260156	RDES	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.025412069168015528	[1016]	[840,1121,1331,1379]	0	hSpCas9	negative selection	8507366
39619357	39619380	22	+	27260156	PC3	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.22772338449502416	[128]	[53,164,255,202]	5	hSpCas9	negative selection	8564334
39619363	39619386	22	-	27260156	PC3	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.2529570838216376	[261]	[207,300,514,313]	5	hSpCas9	negative selection	8564335
39600589	39600612	22	+	27260156	PC3	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.2355062386919049	[666]	[557,833,1061,794]	5	hSpCas9	negative selection	8564336
39570956	39570979	22	-	27260156	PC3	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.24207230850130812	[1292]	[928,1557,2578,1556]	5	hSpCas9	negative selection	8628615
39570794	39570817	22	-	27260156	PC3	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.04351272934526451	[252]	[177,154,562,277]	1	hSpCas9	negative selection	8628616
39600627	39600650	22	-	27260156	PC3	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.17903489959495955	[1016]	[830,1195,1742,1055]	4	hSpCas9	negative selection	8628617
39619357	39619380	22	+	27260156	PATU8988T	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.23454560342093633	[128]	[99,58,150,108]	-4	hSpCas9	negative selection	8685585
39619363	39619386	22	-	27260156	PATU8988T	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.16852080923844426	[261]	[313,327,207,293]	3	hSpCas9	negative selection	8685586
39600589	39600612	22	+	27260156	PATU8988T	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.5665754032707536	[666]	[991,1055,711,1080]	8	hSpCas9	negative selection	8685587
39570956	39570979	22	-	27260156	PATU8988T	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.1007026331425096	[1292]	[1092,1575,1326,1388]	1	hSpCas9	negative selection	8749866
39570794	39570817	22	-	27260156	PATU8988T	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.2836291406924547	[252]	[163,357,159,130]	-5	hSpCas9	negative selection	8749867
39600627	39600650	22	-	27260156	PATU8988T	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.1411623208493431	[1016]	[1259,944,764,1379]	2	hSpCas9	negative selection	8749868
39619357	39619380	22	+	27260156	T47D	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.06458216215144619	[128]	[118,122,154,236]	1	hSpCas9	negative selection	8806836
39619363	39619386	22	-	27260156	T47D	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.22592679548147498	[261]	[321,334,345,428]	5	hSpCas9	negative selection	8806837
39600589	39600612	22	+	27260156	T47D	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.1203136491306589	[666]	[952,742,795,897]	2	hSpCas9	negative selection	8806838
39570956	39570979	22	-	27260156	T47D	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.17111982162956463	[1292]	[1790,1516,1711,1781]	3	hSpCas9	negative selection	8871117
39570794	39570817	22	-	27260156	T47D	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.05471099961017156	[252]	[311,277,340,294]	1	hSpCas9	negative selection	8871118
39600627	39600650	22	-	27260156	T47D	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.003744865625550542	[1016]	[1198,1249,1209,1082]	0	hSpCas9	negative selection	8871119
39619357	39619380	22	+	27260156	SU8686	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.42349434851632073	[128]	[119,137,179,273]	7	hSpCas9	negative selection	8928087
39619363	39619386	22	-	27260156	SU8686	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.16137319974333386	[261]	[345,295,234,270]	2	hSpCas9	negative selection	8928088
39600589	39600612	22	+	27260156	SU8686	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.2695414513403389	[666]	[591,714,939,1007]	4	hSpCas9	negative selection	8928089
39570956	39570979	22	-	27260156	SU8686	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.43099317645046886	[1292]	[1647,1440,1979,1914]	7	hSpCas9	negative selection	8992368
39570794	39570817	22	-	27260156	SU8686	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.024710963590809598	[252]	[182,177,352,297]	0	hSpCas9	negative selection	8992369
39600627	39600650	22	-	27260156	SU8686	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	-0.013479146838391243	[1016]	[861,1049,905,1210]	0	hSpCas9	negative selection	8992370
39619357	39619380	22	+	27260156	SKES1	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.18655843314380594	[128]	[154,183,151,103]	4	hSpCas9	negative selection	9049338
39619363	39619386	22	-	27260156	SKES1	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.23141643346727658	[261]	[223,297,160,215]	-5	hSpCas9	negative selection	9049339
39600589	39600612	22	+	27260156	SKES1	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.17098017117940956	[666]	[874,800,680,665]	3	hSpCas9	negative selection	9049340
39570956	39570979	22	-	27260156	SKES1	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.17250767225067132	[1292]	[1735,1507,1121,1479]	4	hSpCas9	negative selection	9113619
39570794	39570817	22	-	27260156	SKES1	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.03153509693949674	[252]	[231,225,315,209]	0	hSpCas9	negative selection	9113620
39600627	39600650	22	-	27260156	SKES1	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.0013493472478167678	[1016]	[1176,1064,908,938]	0	hSpCas9	negative selection	9113621
39619357	39619380	22	+	27260156	TOV112D	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	-0.01017358775781374	[128]	[118,85,176,119]	0	hSpCas9	negative selection	9170589
39619363	39619386	22	-	27260156	TOV112D	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.3389942581107429	[261]	[366,349,339,278]	6	hSpCas9	negative selection	9170590
39600589	39600612	22	+	27260156	TOV112D	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.36111942537587527	[666]	[1147,1271,531,611]	6	hSpCas9	negative selection	9170591
39570956	39570979	22	-	27260156	TOV112D	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.2768384742498031	[1292]	[1510,1764,1922,1129]	5	hSpCas9	negative selection	9234870
39570794	39570817	22	-	27260156	TOV112D	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.1520980408955499	[252]	[246,127,185,324]	-3	hSpCas9	negative selection	9234871
39600627	39600650	22	-	27260156	TOV112D	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.07492775543624774	[1016]	[801,1272,1089,1148]	1	hSpCas9	negative selection	9234872
39619357	39619380	22	+	27260156	TC71	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.07925209884734419	[128]	[169,179,125,105]	2	hSpCas9	negative selection	9291840
39619363	39619386	22	-	27260156	TC71	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	0.06915699015434862	[261]	[316,320,232,309]	1	hSpCas9	negative selection	9291841
39600589	39600612	22	+	27260156	TC71	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.15458620608763307	[666]	[870,772,776,758]	4	hSpCas9	negative selection	9291842
39570956	39570979	22	-	27260156	TC71	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.04710831951423411	[1292]	[1423,1464,1287,1563]	1	hSpCas9	negative selection	9356121
39570794	39570817	22	-	27260156	TC71	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	0.10456850891324398	[252]	[357,201,323,278]	2	hSpCas9	negative selection	9356122
39600627	39600650	22	-	27260156	TC71	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.020301100373417036	[1016]	[1036,1005,1224,1154]	0	hSpCas9	negative selection	9356123
39619357	39619380	22	+	27260156	TC32	viability	TCCGCACCGTGCGCGTCCTGAGG	CACNA1I	ENSG00000100346	0.1480826729855258	[128]	[104,101,184,163]	2	hSpCas9	negative selection	9413091
39619363	39619386	22	-	27260156	TC32	viability	GAGGGGCCTCAGGACGCGCACGG	CACNA1I	ENSG00000100346	-0.1224185513210927	[261]	[225,134,248,325]	-2	hSpCas9	negative selection	9413092
39600589	39600612	22	+	27260156	TC32	viability	TTTGGCAAGAAGTGCTACCTCGG	CACNA1I	ENSG00000100346	0.22150462212950528	[666]	[731,570,850,809]	4	hSpCas9	negative selection	9413093
39570956	39570979	22	-	27260156	TC32	viability	CCATCTTGATGCACCAGTTCCGG	CACNA1I	ENSG00000100346	0.3160277036283674	[1292]	[1221,1321,1826,1764]	5	hSpCas9	negative selection	9477372
39570794	39570817	22	-	27260156	TC32	viability	TGACTCCTGGCTCAGCGGCTGGG	CACNA1I	ENSG00000100346	-0.05455009454472737	[252]	[159,212,199,344]	-1	hSpCas9	negative selection	9477373
39600627	39600650	22	-	27260156	TC32	viability	CCATGACGATGAAGAAATCCAGG	CACNA1I	ENSG00000100346	0.20302696433062772	[1016]	[861,919,1476,1247]	3	hSpCas9	negative selection	9477374
39570956	39570979	22	+	27869803	HPAFII	viability after 27 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.927580454375704	[0]	[0,0,NaN]	7	hSpCas9	negative selection	9580983
39598181	39598204	22	-	27869803	HPAFII	viability after 27 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.012827740994214354	[114]	[90,35,NaN]	0	hSpCas9	negative selection	9580984
39619397	39619420	22	-	27869803	HPAFII	viability after 27 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	0.01563663104089945	[233]	[155,97,NaN]	0	hSpCas9	negative selection	9580985
39640977	39641000	22	-	27869803	HPAFII	viability after 27 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.21985290599457186	[553]	[218,275,NaN]	-2	hSpCas9	negative selection	9580986
39646592	39646615	22	-	27869803	HPAFII	viability after 27 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.2210559737736339	[323]	[215,183,NaN]	1	hSpCas9	negative selection	9580987
39647900	39647923	22	-	27869803	HPAFII	viability after 27 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.5754428489103147	[339]	[387,164,NaN]	5	hSpCas9	negative selection	9580988
39570956	39570979	22	+	27869803	HPAFII	viability after 15 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	-0.10397428375416773	[0]	[0,0,NaN]	-1	hSpCas9	negative selection	9663432
39598181	39598204	22	-	27869803	HPAFII	viability after 15 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.37701279521031306	[114]	[215,125,NaN]	4	hSpCas9	negative selection	9663433
39619397	39619420	22	-	27869803	HPAFII	viability after 15 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	0.49341774660312293	[233]	[567,220,NaN]	5	hSpCas9	negative selection	9663434
39640977	39641000	22	-	27869803	HPAFII	viability after 15 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.0771559232144825	[553]	[727,565,NaN]	0	hSpCas9	negative selection	9663435
39646592	39646615	22	-	27869803	HPAFII	viability after 15 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.027728861096822266	[323]	[563,224,NaN]	0	hSpCas9	negative selection	9663436
39647900	39647923	22	-	27869803	HPAFII	viability after 15 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.5926358059003229	[339]	[571,537,NaN]	6	hSpCas9	negative selection	9663437
39570956	39570979	22	+	27869803	ASPC1	viability after 36 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	-0.38557732855328997	[1743]	[627,584,252]	-4	hSpCas9	negative selection	9745881
39598181	39598204	22	-	27869803	ASPC1	viability after 36 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.19287437613235325	[267]	[155,182,10]	2	hSpCas9	negative selection	9745882
39619397	39619420	22	-	27869803	ASPC1	viability after 36 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.011273540520996328	[561]	[51,323,189]	0	hSpCas9	negative selection	9745883
39640977	39641000	22	-	27869803	ASPC1	viability after 36 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.3763353021900365	[1442]	[191,588,361]	-4	hSpCas9	negative selection	9745884
39646592	39646615	22	-	27869803	ASPC1	viability after 36 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.8542803588073339	[613]	[506,624,110]	7	hSpCas9	negative selection	9745885
39647900	39647923	22	-	27869803	ASPC1	viability after 36 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.0850038610480981	[995]	[372,602,175]	0	hSpCas9	negative selection	9745886
39570956	39570979	22	+	27869803	PATU8988S	viability after 35 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.07276861445575067	[1307]	[575,531,NaN]	0	hSpCas9	negative selection	9828330
39598181	39598204	22	-	27869803	PATU8988S	viability after 35 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	1.664602546555344	[193]	[117,377,NaN]	9	hSpCas9	negative selection	9828331
39619397	39619420	22	-	27869803	PATU8988S	viability after 35 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	0.08387358783572468	[444]	[168,210,NaN]	1	hSpCas9	negative selection	9828332
39640977	39641000	22	-	27869803	PATU8988S	viability after 35 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.23178844311856195	[1344]	[684,586,NaN]	2	hSpCas9	negative selection	9828333
39646592	39646615	22	-	27869803	PATU8988S	viability after 35 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.34123859495809983	[549]	[333,226,NaN]	4	hSpCas9	negative selection	9828334
39647900	39647923	22	-	27869803	PATU8988S	viability after 35 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.4352336539661909	[949]	[269,295,NaN]	-4	hSpCas9	negative selection	9828335
39570956	39570979	22	+	27869803	PATU8988S	viability after 31 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.361991786443556	[1307]	[591,769,NaN]	4	hSpCas9	negative selection	9910779
39598181	39598204	22	-	27869803	PATU8988S	viability after 31 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	1.4206891231811192	[193]	[75,373,NaN]	9	hSpCas9	negative selection	9910780
39619397	39619420	22	-	27869803	PATU8988S	viability after 31 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.13140491459348413	[444]	[128,203,NaN]	-1	hSpCas9	negative selection	9910781
39640977	39641000	22	-	27869803	PATU8988S	viability after 31 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	-0.0009927724181286735	[1344]	[443,652,NaN]	0	hSpCas9	negative selection	9910782
39646592	39646615	22	-	27869803	PATU8988S	viability after 31 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.6101220715397059	[549]	[288,392,NaN]	7	hSpCas9	negative selection	9910783
39647900	39647923	22	-	27869803	PATU8988S	viability after 31 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	-0.34400888538696994	[949]	[308,284,NaN]	-4	hSpCas9	negative selection	9910784
39570956	39570979	22	+	27869803	HPAFII	viability after 35 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.9820885529966752	[0]	[0,0,NaN]	6	hSpCas9	negative selection	9993228
39598181	39598204	22	-	27869803	HPAFII	viability after 35 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.5333894014103678	[114]	[135,25,NaN]	3	hSpCas9	negative selection	9993229
39619397	39619420	22	-	27869803	HPAFII	viability after 35 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.6413994689937966	[233]	[82,67,NaN]	-4	hSpCas9	negative selection	9993230
39640977	39641000	22	-	27869803	HPAFII	viability after 35 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.1919485760373334	[553]	[238,411,NaN]	1	hSpCas9	negative selection	9993231
39646592	39646615	22	-	27869803	HPAFII	viability after 35 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	0.16997824223703	[323]	[192,174,NaN]	1	hSpCas9	negative selection	9993232
39647900	39647923	22	-	27869803	HPAFII	viability after 35 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.6728509129814053	[339]	[375,159,NaN]	4	hSpCas9	negative selection	9993233
39570956	39570979	22	+	27869803	HPAFII	viability after 31 days	CCGGAACTGGTGCATCAAGATGG	CACNA1I	ENSG00000100346	0.8333711996752582	[0]	[0,0,NaN]	6	hSpCas9	negative selection	10075677
39598181	39598204	22	-	27869803	HPAFII	viability after 31 days	CAAGTGTCACGCAGTTCAGCAGG	CACNA1I	ENSG00000100346	0.7687723787180955	[114]	[164,49,NaN]	5	hSpCas9	negative selection	10075678
39619397	39619420	22	-	27869803	HPAFII	viability after 31 days	GGGCTGACTCACTGGGCACGCGG	CACNA1I	ENSG00000100346	-0.4712657971998756	[233]	[110,76,NaN]	-3	hSpCas9	negative selection	10075679
39640977	39641000	22	-	27869803	HPAFII	viability after 31 days	ACGGCCCTGCTCCTTGAGCGGGG	CACNA1I	ENSG00000100346	0.13461621481757757	[553]	[247,442,NaN]	1	hSpCas9	negative selection	10075680
39646592	39646615	22	-	27869803	HPAFII	viability after 31 days	TCGCTATGACAACGAGGCACAGG	CACNA1I	ENSG00000100346	-0.13348940499096337	[323]	[193,134,NaN]	-1	hSpCas9	negative selection	10075681
39647900	39647923	22	-	27869803	HPAFII	viability after 31 days	TCCCGAGTGATCATTTCCAGGGG	CACNA1I	ENSG00000100346	0.7348202233789392	[339]	[474,145,NaN]	5	hSpCas9	negative selection	10075682
39649689	39649712	22	+	28162770	P31FUJ	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-2.094604910494359	[188]	[51]	-8	hSpCas9	negative selection	10124906
39646855	39646878	22	-	28162770	P31FUJ	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.8213729357366604	[195]	[406]	8	hSpCas9	negative selection	10124907
39660416	39660439	22	+	28162770	P31FUJ	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.25626117723836744	[247]	[243]	-2	hSpCas9	negative selection	10124908
39684489	39684512	22	-	28162770	P31FUJ	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.16680525429485915	[187]	[247]	1	hSpCas9	negative selection	10124909
39570774	39570797	22	-	28162770	P31FUJ	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.21153358766072722	[201]	[204]	-2	hSpCas9	negative selection	10124910
39600555	39600578	22	+	28162770	P31FUJ	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-1.0350381558469692	[210]	[120]	-6	hSpCas9	negative selection	10124911
39634660	39634683	22	+	28162770	P31FUJ	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.813305056944835	[322]	[215]	-6	hSpCas9	negative selection	10124912
39634689	39634712	22	+	28162770	P31FUJ	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.7363763782948796	[447]	[315]	-5	hSpCas9	negative selection	10124913
39642820	39642843	22	+	28162770	P31FUJ	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.3864534738606554	[96]	[148]	4	hSpCas9	negative selection	10124914
39646838	39646861	22	-	28162770	P31FUJ	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.8678017365240271	[269]	[578]	8	hSpCas9	negative selection	10124915
39649689	39649712	22	+	28162770	TF1	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[58]	1	hSpCas9	negative selection	10294272
39646855	39646878	22	-	28162770	TF1	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[264]	1	hSpCas9	negative selection	10294273
39660416	39660439	22	+	28162770	TF1	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[53]	1	hSpCas9	negative selection	10294274
39684489	39684512	22	-	28162770	TF1	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[156]	1	hSpCas9	negative selection	10294275
39570774	39570797	22	-	28162770	TF1	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[75]	1	hSpCas9	negative selection	10294276
39600555	39600578	22	+	28162770	TF1	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[21]	1	hSpCas9	negative selection	10294277
39634660	39634683	22	+	28162770	TF1	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[140]	1	hSpCas9	negative selection	10294278
39634689	39634712	22	+	28162770	TF1	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[311]	1	hSpCas9	negative selection	10294279
39642820	39642843	22	+	28162770	TF1	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[89]	1	hSpCas9	negative selection	10294280
39646838	39646861	22	-	28162770	TF1	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	NaN	[NaN]	[545]	1	hSpCas9	negative selection	10294281
39649689	39649712	22	+	28162770	SKM1	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.3799971511428923	[52]	[35]	-7	hSpCas9	negative selection	10463638
39646855	39646878	22	-	28162770	SKM1	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.15985095526773507	[39]	[78]	1	hSpCas9	negative selection	10463639
39660416	39660439	22	+	28162770	SKM1	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.5863734495288636	[61]	[72]	-5	hSpCas9	negative selection	10463640
39684489	39684512	22	-	28162770	SKM1	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.3143980800070892	[82]	[117]	-3	hSpCas9	negative selection	10463641
39570774	39570797	22	-	28162770	SKM1	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.008770209627732062	[69]	[122]	0	hSpCas9	negative selection	10463642
39600555	39600578	22	+	28162770	SKM1	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.6964708159381465	[65]	[71]	-5	hSpCas9	negative selection	10463643
39634660	39634683	22	+	28162770	SKM1	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.2831356118903033	[52]	[76]	-2	hSpCas9	negative selection	10463644
39634689	39634712	22	+	28162770	SKM1	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.4246662004766207	[81]	[107]	-3	hSpCas9	negative selection	10463645
39642820	39642843	22	+	28162770	SKM1	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.362422873115422	[10]	[24]	3	hSpCas9	negative selection	10463646
39646838	39646861	22	-	28162770	SKM1	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.5894245477044593	[49]	[132]	5	hSpCas9	negative selection	10463647
39649689	39649712	22	+	28162770	SKM1	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.3799971511428923	[52]	[35]	-7	hSpCas9	negative selection	10633004
39646855	39646878	22	-	28162770	SKM1	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.15985095526773507	[39]	[78]	1	hSpCas9	negative selection	10633005
39660416	39660439	22	+	28162770	SKM1	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.5863734495288636	[61]	[72]	-5	hSpCas9	negative selection	10633006
39684489	39684512	22	-	28162770	SKM1	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.3143980800070892	[82]	[117]	-3	hSpCas9	negative selection	10633007
39570774	39570797	22	-	28162770	SKM1	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.008770209627732062	[69]	[122]	0	hSpCas9	negative selection	10633008
39600555	39600578	22	+	28162770	SKM1	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.6964708159381465	[65]	[71]	-5	hSpCas9	negative selection	10633009
39634660	39634683	22	+	28162770	SKM1	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.2831356118903033	[52]	[76]	-2	hSpCas9	negative selection	10633010
39634689	39634712	22	+	28162770	SKM1	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.4246662004766207	[81]	[107]	-3	hSpCas9	negative selection	10633011
39642820	39642843	22	+	28162770	SKM1	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.362422873115422	[10]	[24]	3	hSpCas9	negative selection	10633012
39646838	39646861	22	-	28162770	SKM1	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.5894245477044593	[49]	[132]	5	hSpCas9	negative selection	10633013
39649689	39649712	22	+	28162770	PL21	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	0.710173627703911	[190]	[135]	6	hSpCas9	negative selection	10802370
39646855	39646878	22	-	28162770	PL21	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	-1.1110620738049837	[133]	[26]	-6	hSpCas9	negative selection	10802371
39660416	39660439	22	+	28162770	PL21	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.14651090980927353	[205]	[80]	-1	hSpCas9	negative selection	10802372
39684489	39684512	22	-	28162770	PL21	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.3098850204726633	[290]	[156]	2	hSpCas9	negative selection	10802373
39570774	39570797	22	-	28162770	PL21	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.8139355707224029	[205]	[50]	-5	hSpCas9	negative selection	10802374
39600555	39600578	22	+	28162770	PL21	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	0.8359832842126604	[259]	[201]	7	hSpCas9	negative selection	10802375
39634660	39634683	22	+	28162770	PL21	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.5325765066755281	[225]	[67]	-4	hSpCas9	negative selection	10802376
39634689	39634712	22	+	28162770	PL21	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.11860972899272204	[272]	[128]	1	hSpCas9	negative selection	10802377
39642820	39642843	22	+	28162770	PL21	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	1.3191922861330834	[92]	[100]	9	hSpCas9	negative selection	10802378
39646838	39646861	22	-	28162770	PL21	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.1754775602550511	[117]	[115]	8	hSpCas9	negative selection	10802379
39649689	39649712	22	+	28162770	P31FUJ	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-2.094604910494359	[188]	[51]	-8	hSpCas9	negative selection	10971736
39646855	39646878	22	-	28162770	P31FUJ	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.8213729357366604	[195]	[406]	8	hSpCas9	negative selection	10971737
39660416	39660439	22	+	28162770	P31FUJ	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.25626117723836744	[247]	[243]	-2	hSpCas9	negative selection	10971738
39684489	39684512	22	-	28162770	P31FUJ	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.16680525429485915	[187]	[247]	1	hSpCas9	negative selection	10971739
39570774	39570797	22	-	28162770	P31FUJ	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.21153358766072722	[201]	[204]	-2	hSpCas9	negative selection	10971740
39600555	39600578	22	+	28162770	P31FUJ	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-1.0350381558469692	[210]	[120]	-6	hSpCas9	negative selection	10971741
39634660	39634683	22	+	28162770	P31FUJ	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.813305056944835	[322]	[215]	-6	hSpCas9	negative selection	10971742
39634689	39634712	22	+	28162770	P31FUJ	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.7363763782948796	[447]	[315]	-5	hSpCas9	negative selection	10971743
39642820	39642843	22	+	28162770	P31FUJ	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.3864534738606554	[96]	[148]	4	hSpCas9	negative selection	10971744
39646838	39646861	22	-	28162770	P31FUJ	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.8678017365240271	[269]	[578]	8	hSpCas9	negative selection	10971745
39649689	39649712	22	+	28162770	OCIAML5	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-3.3985549237705173	[110]	[18]	-9	hSpCas9	negative selection	11141102
39646855	39646878	22	-	28162770	OCIAML5	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.2127036845262409	[97]	[204]	1	hSpCas9	negative selection	11141103
39660416	39660439	22	+	28162770	OCIAML5	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.4870697540847486	[65]	[84]	-3	hSpCas9	negative selection	11141104
39684489	39684512	22	-	28162770	OCIAML5	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	1.5953924061072244	[65]	[359]	9	hSpCas9	negative selection	11141105
39570774	39570797	22	-	28162770	OCIAML5	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.40975614143504946	[130]	[177]	-3	hSpCas9	negative selection	11141106
39600555	39600578	22	+	28162770	OCIAML5	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	0.23159135818291243	[66]	[141]	2	hSpCas9	negative selection	11141107
39634660	39634683	22	+	28162770	OCIAML5	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	0.39586094257958837	[135]	[322]	3	hSpCas9	negative selection	11141108
39634689	39634712	22	+	28162770	OCIAML5	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.555865301095004	[214]	[263]	-4	hSpCas9	negative selection	11141109
39642820	39642843	22	+	28162770	OCIAML5	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.4457462886862621	[82]	[109]	-3	hSpCas9	negative selection	11141110
39646838	39646861	22	-	28162770	OCIAML5	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.418215918472665	[90]	[438]	9	hSpCas9	negative selection	11141111
39649689	39649712	22	+	28162770	EOL1	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.4053731140703585	[361]	[71]	-7	hSpCas9	negative selection	11310468
39646855	39646878	22	-	28162770	EOL1	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.8319366213856594	[192]	[180]	7	hSpCas9	negative selection	11310469
39660416	39660439	22	+	28162770	EOL1	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	0.3395852708493784	[335]	[223]	3	hSpCas9	negative selection	11310470
39684489	39684512	22	-	28162770	EOL1	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.2732293505116653	[522]	[227]	-2	hSpCas9	negative selection	11310471
39570774	39570797	22	-	28162770	EOL1	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.20574025763443304	[358]	[163]	-1	hSpCas9	negative selection	11310472
39600555	39600578	22	+	28162770	EOL1	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.5931582907441865	[460]	[160]	-4	hSpCas9	negative selection	11310473
39634660	39634683	22	+	28162770	EOL1	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	1.599395775808886	[279]	[446]	9	hSpCas9	negative selection	11310474
39634689	39634712	22	+	28162770	EOL1	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.2865998925982909	[425]	[183]	-2	hSpCas9	negative selection	11310475
39642820	39642843	22	+	28162770	EOL1	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.4593672102125286	[106]	[40]	-3	hSpCas9	negative selection	11310476
39646838	39646861	22	-	28162770	EOL1	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.5024555147577404	[204]	[152]	4	hSpCas9	negative selection	11310477
39649689	39649712	22	+	28162770	MOLM13	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-0.5847520981966509	[306]	[147]	-3	hSpCas9	negative selection	11479834
39646855	39646878	22	-	28162770	MOLM13	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	-0.2602388493624502	[216]	[130]	-1	hSpCas9	negative selection	11479835
39660416	39660439	22	+	28162770	MOLM13	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.3348091780818038	[231]	[132]	-2	hSpCas9	negative selection	11479836
39684489	39684512	22	-	28162770	MOLM13	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.5615024323160711	[357]	[381]	4	hSpCas9	negative selection	11479837
39570774	39570797	22	-	28162770	MOLM13	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.27601225983200384	[277]	[165]	-2	hSpCas9	negative selection	11479838
39600555	39600578	22	+	28162770	MOLM13	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.7609293089513021	[374]	[159]	-4	hSpCas9	negative selection	11479839
39634660	39634683	22	+	28162770	MOLM13	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.2083221815425309	[309]	[193]	-1	hSpCas9	negative selection	11479840
39634689	39634712	22	+	28162770	MOLM13	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.1022979501846335	[477]	[370]	0	hSpCas9	negative selection	11479841
39642820	39642843	22	+	28162770	MOLM13	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.627669825581348	[110]	[123]	5	hSpCas9	negative selection	11479842
39646838	39646861	22	-	28162770	MOLM13	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.2184130810055067	[232]	[391]	8	hSpCas9	negative selection	11479843
39649689	39649712	22	+	28162770	HEL	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.2399042173614843	[235]	[63]	-6	hSpCas9	negative selection	11649200
39646855	39646878	22	-	28162770	HEL	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	-0.19197655344923975	[172]	[96]	-1	hSpCas9	negative selection	11649201
39660416	39660439	22	+	28162770	HEL	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	1.00603736130758	[219]	[282]	8	hSpCas9	negative selection	11649202
39684489	39684512	22	-	28162770	HEL	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.3638794590048807	[329]	[271]	3	hSpCas9	negative selection	11649203
39570774	39570797	22	-	28162770	HEL	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	0.24540333445497214	[323]	[245]	2	hSpCas9	negative selection	11649204
39600555	39600578	22	+	28162770	HEL	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.9025866964834546	[358]	[122]	-5	hSpCas9	negative selection	11649205
39634660	39634683	22	+	28162770	HEL	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	0.2907535032568146	[290]	[227]	2	hSpCas9	negative selection	11649206
39634689	39634712	22	+	28162770	HEL	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.45994683197420705	[392]	[182]	-3	hSpCas9	negative selection	11649207
39642820	39642843	22	+	28162770	HEL	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-1.4381811633832104	[109]	[25]	-7	hSpCas9	negative selection	11649208
39646838	39646861	22	-	28162770	HEL	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.7669969349625028	[188]	[205]	6	hSpCas9	negative selection	11649209
39649689	39649712	22	+	28162770	MV411	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.0232100391608938	[151]	[62]	-6	hSpCas9	negative selection	11818566
39646855	39646878	22	-	28162770	MV411	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	-0.4035548202735779	[189]	[120]	-3	hSpCas9	negative selection	11818567
39660416	39660439	22	+	28162770	MV411	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-1.054218149078326	[211]	[85]	-6	hSpCas9	negative selection	11818568
39684489	39684512	22	-	28162770	MV411	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.589504167263179	[233]	[130]	-4	hSpCas9	negative selection	11818569
39570774	39570797	22	-	28162770	MV411	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.8012884890336458	[270]	[130]	-5	hSpCas9	negative selection	11818570
39600555	39600578	22	+	28162770	MV411	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.9719429800215673	[318]	[136]	-6	hSpCas9	negative selection	11818571
39634660	39634683	22	+	28162770	MV411	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	0.35519373113865815	[244]	[263]	3	hSpCas9	negative selection	11818572
39634689	39634712	22	+	28162770	MV411	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.10223963524283985	[281]	[254]	0	hSpCas9	negative selection	11818573
39642820	39642843	22	+	28162770	MV411	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.6204589132098797	[72]	[39]	-4	hSpCas9	negative selection	11818574
39646838	39646861	22	-	28162770	MV411	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.2950032340348311	[178]	[184]	2	hSpCas9	negative selection	11818575
39649689	39649712	22	+	28162770	MONOMAC1	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-0.9232760346327011	[133]	[80]	-6	hSpCas9	negative selection	11987932
39646855	39646878	22	-	28162770	MONOMAC1	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	2.6382308207184564	[116]	[834]	9	hSpCas9	negative selection	11987933
39660416	39660439	22	+	28162770	MONOMAC1	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.46007125289334744	[119]	[99]	-4	hSpCas9	negative selection	11987934
39684489	39684512	22	-	28162770	MONOMAC1	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	-0.4194292683960015	[188]	[161]	-4	hSpCas9	negative selection	11987935
39570774	39570797	22	-	28162770	MONOMAC1	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-0.5061409015893323	[139]	[112]	-4	hSpCas9	negative selection	11987936
39600555	39600578	22	+	28162770	MONOMAC1	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	0.15541611484126716	[158]	[202]	1	hSpCas9	negative selection	11987937
39634660	39634683	22	+	28162770	MONOMAC1	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.17612785729657293	[136]	[138]	-1	hSpCas9	negative selection	11987938
39634689	39634712	22	+	28162770	MONOMAC1	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	-0.2331061017670755	[161]	[157]	-2	hSpCas9	negative selection	11987939
39642820	39642843	22	+	28162770	MONOMAC1	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	0.2659351292829921	[36]	[50]	2	hSpCas9	negative selection	11987940
39646838	39646861	22	-	28162770	MONOMAC1	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	0.6298984439675669	[114]	[203]	6	hSpCas9	negative selection	11987941
39649689	39649712	22	+	28162770	OCIAML2	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-1.583840435690152	[52]	[97]	-7	hSpCas9	negative selection	12157298
39646855	39646878	22	-	28162770	OCIAML2	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.34565822158545073	[22]	[161]	3	hSpCas9	negative selection	12157299
39660416	39660439	22	+	28162770	OCIAML2	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.05109093372837645	[39]	[213]	0	hSpCas9	negative selection	12157300
39684489	39684512	22	-	28162770	OCIAML2	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.6612818502634199	[46]	[411]	6	hSpCas9	negative selection	12157301
39570774	39570797	22	-	28162770	OCIAML2	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	-1.1964548618031672	[42]	[103]	-7	hSpCas9	negative selection	12157302
39600555	39600578	22	+	28162770	OCIAML2	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-1.6680758894483783	[42]	[74]	-7	hSpCas9	negative selection	12157303
39634660	39634683	22	+	28162770	OCIAML2	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	0.45098227607312275	[25]	[196]	4	hSpCas9	negative selection	12157304
39634689	39634712	22	+	28162770	OCIAML2	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.702497608238495	[49]	[450]	6	hSpCas9	negative selection	12157305
39642820	39642843	22	+	28162770	OCIAML2	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.36371462132564947	[12]	[55]	-3	hSpCas9	negative selection	12157306
39646838	39646861	22	-	28162770	OCIAML2	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.3650697225094046	[17]	[256]	9	hSpCas9	negative selection	12157307
39649689	39649712	22	+	28162770	OCIAML3	viability	GAGGACGAGGCGGATGGGGACGG	CACNA1I	ENSG00000100346	-0.5505060016875767	[48]	[100]	-4	hSpCas9	negative selection	12326664
39646855	39646878	22	-	28162770	OCIAML3	viability	GTGCCGGGGCTCCTTGGCGTGGG	CACNA1I	ENSG00000100346	0.4059923596758368	[44]	[179]	4	hSpCas9	negative selection	12326665
39660416	39660439	22	+	28162770	OCIAML3	viability	GAAGGCCTGGACAGCAGCGGAGG	CACNA1I	ENSG00000100346	-0.14262829398159896	[48]	[133]	-1	hSpCas9	negative selection	12326666
39684489	39684512	22	-	28162770	OCIAML3	viability	GAGGTGTCGGTACCTGCCGGAGG	CACNA1I	ENSG00000100346	0.43900741690379996	[53]	[220]	4	hSpCas9	negative selection	12326667
39570774	39570797	22	-	28162770	OCIAML3	viability	GGGGCTGCTGCAGATGAGGAGGG	CACNA1I	ENSG00000100346	0.8150060713269105	[44]	[238]	7	hSpCas9	negative selection	12326668
39600555	39600578	22	+	28162770	OCIAML3	viability	GGAGATGGTGCTCAAGATGGTGG	CACNA1I	ENSG00000100346	-0.097854406560863	[66]	[188]	0	hSpCas9	negative selection	12326669
39634660	39634683	22	+	28162770	OCIAML3	viability	ATAGGTGTGCAGCTCTGGGCGGG	CACNA1I	ENSG00000100346	-0.6585478925188738	[79]	[152]	-5	hSpCas9	negative selection	12326670
39634689	39634712	22	+	28162770	OCIAML3	viability	GCGTAACCGCTGCTTCCTGGAGG	CACNA1I	ENSG00000100346	0.45208877426529104	[76]	[317]	4	hSpCas9	negative selection	12326671
39642820	39642843	22	+	28162770	OCIAML3	viability	GGAGATCATGTACTACGTGATGG	CACNA1I	ENSG00000100346	-0.8894635238503342	[26]	[43]	-6	hSpCas9	negative selection	12326672
39646838	39646861	22	-	28162770	OCIAML3	viability	CGTGGGGCCCAGGTTTGGCGGGG	CACNA1I	ENSG00000100346	1.4288323360339483	[46]	[381]	9	hSpCas9	negative selection	12326673