| 130430571 | 130430594 | 9 | - | 26627737 | DLD1 | viability | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.4232985612072355 | [1211] | [661,666,242] | 4 | hSpCas9 | negative selection | 841677 | 130430583 | 130430606 | 9 | + | 26627737 | DLD1 | viability | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.7849793344285078 | [158] | [92,155,20] | 7 | hSpCas9 | negative selection | 841678 | 130431406 | 130431429 | 9 | - | 26627737 | DLD1 | viability | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.3913782224216229 | [391] | [200,81,190] | 4 | hSpCas9 | negative selection | 841679 | 130432449 | 130432472 | 9 | + | 26627737 | DLD1 | viability | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.24809742719179756 | [368] | [118,141,40] | -3 | hSpCas9 | negative selection | 841680 | 130432521 | 130432544 | 9 | + | 26627737 | DLD1 | viability | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.03676644008848651 | [929] | [93,434,321] | 0 | hSpCas9 | negative selection | 841681 | 130433602 | 130433625 | 9 | - | 26627737 | DLD1 | viability | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.15178322652541862 | [509] | [33,271,131] | -2 | hSpCas9 | negative selection | 841682 | 130430571 | 130430594 | 9 | - | 26627737 | GBM cells | viability after 5 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.23776654545887665 | [660] | [399,435] | 4 | hSpCas9 | negative selection | 923992 | 130430583 | 130430606 | 9 | + | 26627737 | GBM cells | viability after 5 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.6785238064603853 | [59] | [32,69] | 8 | hSpCas9 | negative selection | 923993 | 130431406 | 130431429 | 9 | - | 26627737 | GBM cells | viability after 5 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.10005374699353708 | [126] | [86,58] | 1 | hSpCas9 | negative selection | 923994 | 130432449 | 130432472 | 9 | + | 26627737 | GBM cells | viability after 5 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.06354457665848501 | [90] | [74,26] | 1 | hSpCas9 | negative selection | 923995 | 130432521 | 130432544 | 9 | + | 26627737 | GBM cells | viability after 5 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.07284080688549432 | [319] | [205,154] | 1 | hSpCas9 | negative selection | 923996 | 130433602 | 130433625 | 9 | - | 26627737 | GBM cells | viability after 5 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.7347639108684986 | [255] | [74,89] | -8 | hSpCas9 | negative selection | 923997 | 130430571 | 130430594 | 9 | - | 26627737 | GBM cells | viability after 13 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.0744352220227813 | [660] | [402,356] | 1 | hSpCas9 | negative selection | 1006307 | 130430583 | 130430606 | 9 | + | 26627737 | GBM cells | viability after 13 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.3221172883885042 | [59] | [59,24] | 4 | hSpCas9 | negative selection | 1006308 | 130431406 | 130431429 | 9 | - | 26627737 | GBM cells | viability after 13 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.3905658328054694 | [126] | [134,53] | 5 | hSpCas9 | negative selection | 1006309 | 130432449 | 130432472 | 9 | + | 26627737 | GBM cells | viability after 13 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.12269867857390881 | [90] | [50,55] | 1 | hSpCas9 | negative selection | 1006310 | 130432521 | 130432544 | 9 | + | 26627737 | GBM cells | viability after 13 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.21959385358915 | [319] | [194,207] | 3 | hSpCas9 | negative selection | 1006311 | 130433602 | 130433625 | 9 | - | 26627737 | GBM cells | viability after 13 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.15033153889761497 | [255] | [85,156] | -2 | hSpCas9 | negative selection | 1006312 | 130430571 | 130430594 | 9 | - | 26627737 | GBM cells | viability after 21 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.5491822766372818 | [660] | [584,470] | 5 | hSpCas9 | negative selection | 1088622 | 130430583 | 130430606 | 9 | + | 26627737 | GBM cells | viability after 21 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.3293299633081783 | [59] | [41,39] | 3 | hSpCas9 | negative selection | 1088623 | 130431406 | 130431429 | 9 | - | 26627737 | GBM cells | viability after 21 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.6319976890553842 | [126] | [43,44] | -5 | hSpCas9 | negative selection | 1088624 | 130432449 | 130432472 | 9 | + | 26627737 | GBM cells | viability after 21 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.07287837755805415 | [90] | [51,51] | 0 | hSpCas9 | negative selection | 1088625 | 130432521 | 130432544 | 9 | + | 26627737 | GBM cells | viability after 21 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.33243334902187976 | [319] | [232,205] | 3 | hSpCas9 | negative selection | 1088626 | 130433602 | 130433625 | 9 | - | 26627737 | GBM cells | viability after 21 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.7876555382272774 | [255] | [78,81] | -6 | hSpCas9 | negative selection | 1088627 | 130430571 | 130430594 | 9 | - | 26627737 | RPE1 | viability after 9 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.33001242793701846 | [2322] | [694,1196] | 2 | hSpCas9 | negative selection | 1170937 | 130430583 | 130430606 | 9 | + | 26627737 | RPE1 | viability after 9 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 3.0067722676378916 | [49] | [127,122] | 9 | hSpCas9 | negative selection | 1170938 | 130431406 | 130431429 | 9 | - | 26627737 | RPE1 | viability after 9 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 2.216331031909167 | [236] | [339,350] | 9 | hSpCas9 | negative selection | 1170939 | 130432449 | 130432472 | 9 | + | 26627737 | RPE1 | viability after 9 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.39067543414177 | [464] | [131,83] | -3 | hSpCas9 | negative selection | 1170940 | 130432521 | 130432544 | 9 | + | 26627737 | RPE1 | viability after 9 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.07563141521155925 | [1227] | [412,394] | 0 | hSpCas9 | negative selection | 1170941 | 130433602 | 130433625 | 9 | - | 26627737 | RPE1 | viability after 9 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.30302199786018386 | [500] | [165,228] | 2 | hSpCas9 | negative selection | 1170942 | 130430571 | 130430594 | 9 | - | 26627737 | RPE1 | viability after 12 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.28066388421834065 | [2322] | [711,806] | 2 | hSpCas9 | negative selection | 1253252 | 130430583 | 130430606 | 9 | + | 26627737 | RPE1 | viability after 12 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 2.7060549724563803 | [49] | [126,45] | 9 | hSpCas9 | negative selection | 1253253 | 130431406 | 130431429 | 9 | - | 26627737 | RPE1 | viability after 12 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 2.0806681585797095 | [236] | [263,274] | 9 | hSpCas9 | negative selection | 1253254 | 130432449 | 130432472 | 9 | + | 26627737 | RPE1 | viability after 12 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.06566173420011412 | [464] | [80,159] | 0 | hSpCas9 | negative selection | 1253255 | 130432521 | 130432544 | 9 | + | 26627737 | RPE1 | viability after 12 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.5338481948270316 | [1227] | [550,399] | 3 | hSpCas9 | negative selection | 1253256 | 130433602 | 130433625 | 9 | - | 26627737 | RPE1 | viability after 12 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.827103644855372 | [500] | [265,209] | 5 | hSpCas9 | negative selection | 1253257 | 130430571 | 130430594 | 9 | - | 26627737 | RPE1 | viability after 15 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.5297133838038497 | [2322] | [714,1173] | 3 | hSpCas9 | negative selection | 1335567 | 130430583 | 130430606 | 9 | + | 26627737 | RPE1 | viability after 15 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 3.2830783075205296 | [49] | [239,38] | 9 | hSpCas9 | negative selection | 1335568 | 130431406 | 130431429 | 9 | - | 26627737 | RPE1 | viability after 15 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 1.9501541367491788 | [236] | [339,180] | 8 | hSpCas9 | negative selection | 1335569 | 130432449 | 130432472 | 9 | + | 26627737 | RPE1 | viability after 15 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.36059053192376944 | [464] | [20,180] | -2 | hSpCas9 | negative selection | 1335570 | 130432521 | 130432544 | 9 | + | 26627737 | RPE1 | viability after 15 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.8722752229142848 | [1227] | [782,493] | 5 | hSpCas9 | negative selection | 1335571 | 130433602 | 130433625 | 9 | - | 26627737 | RPE1 | viability after 15 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.9996075619120584 | [500] | [318,248] | 6 | hSpCas9 | negative selection | 1335572 | 130430571 | 130430594 | 9 | - | 26627737 | RPE1 | viability after 18 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.6268498804629279 | [2322] | [998,934] | 4 | hSpCas9 | negative selection | 1417882 | 130430583 | 130430606 | 9 | + | 26627737 | RPE1 | viability after 18 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 1.8839133667524646 | [49] | [43,55] | 8 | hSpCas9 | negative selection | 1417883 | 130431406 | 130431429 | 9 | - | 26627737 | RPE1 | viability after 18 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 1.3562656687845598 | [236] | [272,46] | 7 | hSpCas9 | negative selection | 1417884 | 130432449 | 130432472 | 9 | + | 26627737 | RPE1 | viability after 18 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.6773641636889842 | [464] | [62,94] | -4 | hSpCas9 | negative selection | 1417885 | 130432521 | 130432544 | 9 | + | 26627737 | RPE1 | viability after 18 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.6989722311176719 | [1227] | [580,491] | 4 | hSpCas9 | negative selection | 1417886 | 130433602 | 130433625 | 9 | - | 26627737 | RPE1 | viability after 18 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.4695038345003043 | [500] | [134,242] | 3 | hSpCas9 | negative selection | 1417887 | 130430571 | 130430594 | 9 | - | 26627737 | HeLa | viability after 8 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.035224850538537744 | [1041] | [530,494,387] | 0 | hSpCas9 | negative selection | 1500197 | 130430583 | 130430606 | 9 | + | 26627737 | HeLa | viability after 8 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.9106809525348611 | [188] | [65,53,17] | -7 | hSpCas9 | negative selection | 1500198 | 130431406 | 130431429 | 9 | - | 26627737 | HeLa | viability after 8 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 1.2134175331287327 | [220] | [188,256,215] | 8 | hSpCas9 | negative selection | 1500199 | 130432449 | 130432472 | 9 | + | 26627737 | HeLa | viability after 8 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.9601068725444464 | [57] | [44,1,95] | 7 | hSpCas9 | negative selection | 1500200 | 130432521 | 130432544 | 9 | + | 26627737 | HeLa | viability after 8 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.41770541057256705 | [509] | [335,205,351] | 4 | hSpCas9 | negative selection | 1500201 | 130433602 | 130433625 | 9 | - | 26627737 | HeLa | viability after 8 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.599861772020494 | [207] | [328,304,205] | 9 | hSpCas9 | negative selection | 1500202 | 130430571 | 130430594 | 9 | - | 26627737 | HeLa | viability after 12 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | -0.07260263992871296 | [1041] | [362,419,442] | 0 | hSpCas9 | negative selection | 1582512 | 130430583 | 130430606 | 9 | + | 26627737 | HeLa | viability after 12 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.5494244204003782 | [188] | [124,7,33] | -5 | hSpCas9 | negative selection | 1582513 | 130431406 | 130431429 | 9 | - | 26627737 | HeLa | viability after 12 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.8050823842961743 | [220] | [158,71,256] | 6 | hSpCas9 | negative selection | 1582514 | 130432449 | 130432472 | 9 | + | 26627737 | HeLa | viability after 12 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 1.4469268369076875 | [57] | [48,98,43] | 8 | hSpCas9 | negative selection | 1582515 | 130432521 | 130432544 | 9 | + | 26627737 | HeLa | viability after 12 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.5051023362521048 | [509] | [227,61,166] | -4 | hSpCas9 | negative selection | 1582516 | 130433602 | 130433625 | 9 | - | 26627737 | HeLa | viability after 12 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.2806443150793958 | [207] | [242,222,159] | 8 | hSpCas9 | negative selection | 1582517 | 130430571 | 130430594 | 9 | - | 26627737 | HeLa | viability after 15 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | -0.24490031028915937 | [1041] | [354,287,436] | -2 | hSpCas9 | negative selection | 1664827 | 130430583 | 130430606 | 9 | + | 26627737 | HeLa | viability after 15 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -2.114414921253273 | [188] | [0,28,23] | -9 | hSpCas9 | negative selection | 1664828 | 130431406 | 130431429 | 9 | - | 26627737 | HeLa | viability after 15 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.7333613373049405 | [220] | [67,144,243] | 6 | hSpCas9 | negative selection | 1664829 | 130432449 | 130432472 | 9 | + | 26627737 | HeLa | viability after 15 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 2.129925722768867 | [57] | [169,25,113] | 9 | hSpCas9 | negative selection | 1664830 | 130432521 | 130432544 | 9 | + | 26627737 | HeLa | viability after 15 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.38981924679484686 | [509] | [116,114,250] | -3 | hSpCas9 | negative selection | 1664831 | 130433602 | 130433625 | 9 | - | 26627737 | HeLa | viability after 15 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.4458817126863943 | [207] | [325,119,245] | 8 | hSpCas9 | negative selection | 1664832 | 130430571 | 130430594 | 9 | - | 26627737 | HeLa | viability after 18 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | -0.2197820749219881 | [1041] | [241,248,436] | -2 | hSpCas9 | negative selection | 1747142 | 130430583 | 130430606 | 9 | + | 26627737 | HeLa | viability after 18 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -2.0364659491520243 | [188] | [0,25,23] | -9 | hSpCas9 | negative selection | 1747143 | 130431406 | 130431429 | 9 | - | 26627737 | HeLa | viability after 18 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.749746585938291 | [220] | [44,124,243] | 6 | hSpCas9 | negative selection | 1747144 | 130432449 | 130432472 | 9 | + | 26627737 | HeLa | viability after 18 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 2.0781439358866036 | [57] | [104,23,113] | 9 | hSpCas9 | negative selection | 1747145 | 130432521 | 130432544 | 9 | + | 26627737 | HeLa | viability after 18 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.3948904484383985 | [509] | [83,86,250] | -3 | hSpCas9 | negative selection | 1747146 | 130433602 | 130433625 | 9 | - | 26627737 | HeLa | viability after 18 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.5093282525561706 | [207] | [235,102,245] | 8 | hSpCas9 | negative selection | 1747147 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 6 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 1.665316713287338 | [1131] | [499,599] | 8 | hSpCas9 | negative selection | 1829457 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 6 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.1738987219464414 | [249] | [24,54] | 1 | hSpCas9 | negative selection | 1829458 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 6 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -1.0737111119971239 | [539] | [49,34] | -6 | hSpCas9 | negative selection | 1829459 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 6 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -1.47618417302469 | [621] | [87,5] | -7 | hSpCas9 | negative selection | 1829460 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 6 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.35739277234044275 | [936] | [230,50] | -2 | hSpCas9 | negative selection | 1829461 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 6 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.1883582755324276 | [553] | [260,163] | 6 | hSpCas9 | negative selection | 1829462 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 8 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.039909732595249325 | [841] | [580,391,459] | 0 | hSpCas9 | negative selection | 1911772 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 8 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.40185792056374425 | [137] | [75,39,56] | -5 | hSpCas9 | negative selection | 1911773 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 8 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.5186166410419144 | [164] | [103,132,152] | 5 | hSpCas9 | negative selection | 1911774 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 8 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.9573180307664344 | [76] | [65,109,70] | 8 | hSpCas9 | negative selection | 1911775 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 8 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.02594339152596503 | [463] | [270,155,352] | 0 | hSpCas9 | negative selection | 1911776 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 8 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.04009460076423704 | [222] | [66,121,167] | 0 | hSpCas9 | negative selection | 1911777 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 9 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.9573489714219205 | [1131] | [725,476] | 6 | hSpCas9 | negative selection | 1994087 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 9 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -1.5605895907670975 | [249] | [49,0] | -8 | hSpCas9 | negative selection | 1994088 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 9 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.17854310807614457 | [539] | [237,39] | -1 | hSpCas9 | negative selection | 1994089 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 9 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.6562225513865829 | [621] | [177,47] | -4 | hSpCas9 | negative selection | 1994090 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 9 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.990448105694623 | [936] | [438,542] | 6 | hSpCas9 | negative selection | 1994091 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 9 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.35135666840352553 | [553] | [95,261] | 2 | hSpCas9 | negative selection | 1994092 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 12 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.07625197470888545 | [841] | [590,276,515] | 0 | hSpCas9 | negative selection | 2076402 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 12 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.8318104531175678 | [137] | [30,36,54] | -7 | hSpCas9 | negative selection | 2076403 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 12 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.3665881422008835 | [164] | [84,80,170] | 3 | hSpCas9 | negative selection | 2076404 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 12 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 1.2805435977185073 | [76] | [65,149,82] | 8 | hSpCas9 | negative selection | 2076405 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 12 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | 0.7452821023499643 | [463] | [418,310,492] | 6 | hSpCas9 | negative selection | 2076406 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 12 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.18470883848975506 | [222] | [90,86,131] | -2 | hSpCas9 | negative selection | 2076407 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 15 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.16114049107112294 | [841] | [658,395,580] | 1 | hSpCas9 | negative selection | 2158717 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 15 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -2.675869583974144 | [137] | [26,0,10] | -9 | hSpCas9 | negative selection | 2158718 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 15 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.2678080902951322 | [164] | [78,65,90] | -3 | hSpCas9 | negative selection | 2158719 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 15 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 1.6811410922039196 | [76] | [65,274,75] | 9 | hSpCas9 | negative selection | 2158720 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 15 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.017476304423674982 | [463] | [481,119,214] | 0 | hSpCas9 | negative selection | 2158721 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 15 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.6437353081807242 | [222] | [204,206,188] | 6 | hSpCas9 | negative selection | 2158722 | 130430571 | 130430594 | 9 | - | 26627737 | HCT116 | viability after 18 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.1454938890527795 | [841] | [481,383,490] | 1 | hSpCas9 | negative selection | 2241032 | 130430583 | 130430606 | 9 | + | 26627737 | HCT116 | viability after 18 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -4.111748338669026 | [137] | [1,0,8] | -9 | hSpCas9 | negative selection | 2241033 | 130431406 | 130431429 | 9 | - | 26627737 | HCT116 | viability after 18 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.024677128516073954 | [164] | [84,108,45] | 0 | hSpCas9 | negative selection | 2241034 | 130432449 | 130432472 | 9 | + | 26627737 | HCT116 | viability after 18 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 2.033455406598564 | [76] | [28,315,98] | 9 | hSpCas9 | negative selection | 2241035 | 130432521 | 130432544 | 9 | + | 26627737 | HCT116 | viability after 18 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.33747550967063933 | [463] | [163,153,217] | -3 | hSpCas9 | negative selection | 2241036 | 130433602 | 130433625 | 9 | - | 26627737 | HCT116 | viability after 18 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.4412583519181683 | [222] | [52,48,352] | 4 | hSpCas9 | negative selection | 2241037 | 130334728 | 130334751 | 9 | - | 25494202 | A375 | resistance to PLX-4720 (puromycin) | GAGAAAGAGAAAGAGAGAGAAAG | HMCN2 | ENSG00000148357 | 0.039126650339510544 | [163,24] | [27,12] | 0 | dCas9-VP64 | positive selection | 2385787 | 130397370 | 130397393 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (puromycin) | GGGAGCTACAAGATGAGATTTGG | HMCN2 | ENSG00000148357 | 0.9275899236952089 | [95,36] | [24,29] | 4 | dCas9-VP64 | positive selection | 2389500 | 130302079 | 130302102 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (puromycin) | TAGCCCAGGCTGGAGTGTAGTGG | HMCN2 | ENSG00000148357 | 1.7114406221969245 | [69,25] | [57,8] | 6 | dCas9-VP64 | positive selection | 2392329 | 130416294 | 130416317 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (puromycin) | AGGGTTTCACCATGTTGGCCCGG | HMCN2 | ENSG00000148357 | 0.051094447397205506 | [27,110] | [6,24] | 0 | dCas9-VP64 | positive selection | 2397855 | 130357079 | 130357102 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (puromycin) | TGGATGGATGGATGGATGGAAAG | HMCN2 | ENSG00000148357 | 2.7644531499590617 | [51,2] | [77,0] | 8 | dCas9-VP64 | positive selection | 2471239 | 130356963 | 130356986 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (puromycin) | TGGATGGATGGATGGATGGAAAG | HMCN2 | ENSG00000148357 | 2.7644531499590617 | [51,2] | [77,0] | 8 | dCas9-VP64 | positive selection | 2471245 | 130334728 | 130334751 | 9 | - | 25494202 | A375 | resistance to PLX-4720 (zeocin) | GAGAAAGAGAAAGAGAGAGAAAG | HMCN2 | ENSG00000148357 | 1.133413249914112 | [120,136] | [88,94] | 7 | dCas9-VP64 | positive selection | 2481042 | 130397370 | 130397393 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (zeocin) | GGGAGCTACAAGATGAGATTTGG | HMCN2 | ENSG00000148357 | 0.0345862241759054 | [177,72] | [58,25] | 0 | dCas9-VP64 | positive selection | 2484755 | 130302079 | 130302102 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (zeocin) | TAGCCCAGGCTGGAGTGTAGTGG | HMCN2 | ENSG00000148357 | 1.81784862797452 | [47,48] | [40,70] | 8 | dCas9-VP64 | positive selection | 2487584 | 130416294 | 130416317 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (zeocin) | AGGGTTTCACCATGTTGGCCCGG | HMCN2 | ENSG00000148357 | -0.16706931973118405 | [70,280] | [30,67] | -2 | dCas9-VP64 | positive selection | 2493110 | 130357079 | 130357102 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (zeocin) | TGGATGGATGGATGGATGGAAAG | HMCN2 | ENSG00000148357 | -0.745179068402318 | [185,151] | [42,22] | -7 | dCas9-VP64 | positive selection | 2566494 | 130356963 | 130356986 | 9 | + | 25494202 | A375 | resistance to PLX-4720 (zeocin) | TGGATGGATGGATGGATGGAAAG | HMCN2 | ENSG00000148357 | -0.745179068402318 | [185,151] | [42,22] | -7 | dCas9-VP64 | positive selection | 2566500 | 130348568 | 130348591 | 9 | + | 27661255 | K562 | viability | GGGCGCAAGGCCAACGTGTCGGG | HMCN2 | ENSG00000148357 | 0.21310095098492376 | [904,1148] | [1076,835] | 7 | dCas9-KRAB | negative selection | 5485034 | 130348999 | 130349022 | 9 | + | 27661255 | K562 | viability | GGCCACCGCCTCCTGGACGAGGG | HMCN2 | ENSG00000148357 | 0.15377322402948768 | [334,588] | [453,365] | 5 | dCas9-KRAB | negative selection | 5485035 | 130348948 | 130348971 | 9 | - | 27661255 | K562 | viability | GCCAGAGGGAGCCAGTAAGAGGG | HMCN2 | ENSG00000148357 | -0.26933007021373667 | [422,568] | [414,251] | -6 | dCas9-KRAB | negative selection | 5485036 | 130430571 | 130430594 | 9 | - | 27869803 | HPAFII | viability after 27 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.2677464800416698 | [665] | [420,421,NaN] | 2 | hSpCas9 | negative selection | 9604782 | 130430583 | 130430606 | 9 | + | 27869803 | HPAFII | viability after 27 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.20992306937423133 | [62] | [44,14,NaN] | -1 | hSpCas9 | negative selection | 9604783 | 130431406 | 130431429 | 9 | - | 27869803 | HPAFII | viability after 27 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.7381359187464094 | [166] | [92,18,NaN] | -5 | hSpCas9 | negative selection | 9604784 | 130432449 | 130432472 | 9 | + | 27869803 | HPAFII | viability after 27 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -4.103757512157303 | [109] | [4,1,NaN] | -9 | hSpCas9 | negative selection | 9604785 | 130432521 | 130432544 | 9 | + | 27869803 | HPAFII | viability after 27 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.35113557369036 | [443] | [194,172,NaN] | -3 | hSpCas9 | negative selection | 9604786 | 130433602 | 130433625 | 9 | - | 27869803 | HPAFII | viability after 27 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.6454118815851944 | [206] | [418,274,NaN] | 9 | hSpCas9 | negative selection | 9604787 | 130430571 | 130430594 | 9 | - | 27869803 | HPAFII | viability after 15 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.2941814948467829 | [665] | [1082,749,NaN] | 3 | hSpCas9 | negative selection | 9687231 | 130430583 | 130430606 | 9 | + | 27869803 | HPAFII | viability after 15 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.17304726459047326 | [62] | [109,28,NaN] | -2 | hSpCas9 | negative selection | 9687232 | 130431406 | 130431429 | 9 | - | 27869803 | HPAFII | viability after 15 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.1769935432109695 | [166] | [241,106,NaN] | -2 | hSpCas9 | negative selection | 9687233 | 130432449 | 130432472 | 9 | + | 27869803 | HPAFII | viability after 15 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -0.335743190356089 | [109] | [179,39,NaN] | -4 | hSpCas9 | negative selection | 9687234 | 130432521 | 130432544 | 9 | + | 27869803 | HPAFII | viability after 15 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.11149713605634815 | [443] | [519,392,NaN] | -1 | hSpCas9 | negative selection | 9687235 | 130433602 | 130433625 | 9 | - | 27869803 | HPAFII | viability after 15 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.0085047552848052 | [206] | [470,432,NaN] | 9 | hSpCas9 | negative selection | 9687236 | 130430571 | 130430594 | 9 | - | 27869803 | ASPC1 | viability after 36 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.24932400914007657 | [1471] | [801,445,600] | 2 | hSpCas9 | negative selection | 9769680 | 130430583 | 130430606 | 9 | + | 27869803 | ASPC1 | viability after 36 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.49610396854431627 | [208] | [13,281,21] | 5 | hSpCas9 | negative selection | 9769681 | 130431406 | 130431429 | 9 | - | 27869803 | ASPC1 | viability after 36 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -1.4928725389282878 | [412] | [44,78,32] | -8 | hSpCas9 | negative selection | 9769682 | 130432449 | 130432472 | 9 | + | 27869803 | ASPC1 | viability after 36 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.22523940620416227 | [188] | [76,93,62] | 2 | hSpCas9 | negative selection | 9769683 | 130432521 | 130432544 | 9 | + | 27869803 | ASPC1 | viability after 36 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.10310865363752875 | [1122] | [417,379,311] | -1 | hSpCas9 | negative selection | 9769684 | 130433602 | 130433625 | 9 | - | 27869803 | ASPC1 | viability after 36 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.668568457419766 | [476] | [664,684,304] | 9 | hSpCas9 | negative selection | 9769685 | 130430571 | 130430594 | 9 | - | 27869803 | PATU8988S | viability after 35 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | -0.023205179225561423 | [1232] | [561,414,NaN] | 0 | hSpCas9 | negative selection | 9852129 | 130430583 | 130430606 | 9 | + | 27869803 | PATU8988S | viability after 35 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.08788704734094988 | [160] | [31,105,NaN] | 1 | hSpCas9 | negative selection | 9852130 | 130431406 | 130431429 | 9 | - | 27869803 | PATU8988S | viability after 35 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 1.125549839605615 | [256] | [153,297,NaN] | 9 | hSpCas9 | negative selection | 9852131 | 130432449 | 130432472 | 9 | + | 27869803 | PATU8988S | viability after 35 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.535647311700899 | [210] | [204,40,NaN] | 6 | hSpCas9 | negative selection | 9852132 | 130432521 | 130432544 | 9 | + | 27869803 | PATU8988S | viability after 35 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.26422408656419827 | [999] | [487,181,NaN] | -3 | hSpCas9 | negative selection | 9852133 | 130433602 | 130433625 | 9 | - | 27869803 | PATU8988S | viability after 35 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.481574881446794 | [500] | [384,177,NaN] | 5 | hSpCas9 | negative selection | 9852134 | 130430571 | 130430594 | 9 | - | 27869803 | PATU8988S | viability after 31 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.07196730273133145 | [1232] | [507,527,NaN] | 0 | hSpCas9 | negative selection | 9934578 | 130430583 | 130430606 | 9 | + | 27869803 | PATU8988S | viability after 31 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 1.0381589188014662 | [160] | [115,151,NaN] | 9 | hSpCas9 | negative selection | 9934579 | 130431406 | 130431429 | 9 | - | 27869803 | PATU8988S | viability after 31 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | 0.9773850609704775 | [256] | [38,408,NaN] | 9 | hSpCas9 | negative selection | 9934580 | 130432449 | 130432472 | 9 | + | 27869803 | PATU8988S | viability after 31 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | 0.7860890781687412 | [210] | [152,134,NaN] | 8 | hSpCas9 | negative selection | 9934581 | 130432521 | 130432544 | 9 | + | 27869803 | PATU8988S | viability after 31 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.11743627353588737 | [999] | [422,296,NaN] | -1 | hSpCas9 | negative selection | 9934582 | 130433602 | 130433625 | 9 | - | 27869803 | PATU8988S | viability after 31 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | -0.07895359965292131 | [500] | [244,117,NaN] | -1 | hSpCas9 | negative selection | 9934583 | 130430571 | 130430594 | 9 | - | 27869803 | HPAFII | viability after 35 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.2917965976846524 | [665] | [479,336,NaN] | 1 | hSpCas9 | negative selection | 10017027 | 130430583 | 130430606 | 9 | + | 27869803 | HPAFII | viability after 35 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | -0.35773599318424076 | [62] | [38,8,NaN] | -2 | hSpCas9 | negative selection | 10017028 | 130431406 | 130431429 | 9 | - | 27869803 | HPAFII | viability after 35 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -1.153088103295983 | [166] | [69,1,NaN] | -6 | hSpCas9 | negative selection | 10017029 | 130432449 | 130432472 | 9 | + | 27869803 | HPAFII | viability after 35 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -2.0165642475711936 | [109] | [9,17,NaN] | -7 | hSpCas9 | negative selection | 10017030 | 130432521 | 130432544 | 9 | + | 27869803 | HPAFII | viability after 35 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.6754071025954717 | [443] | [96,189,NaN] | -4 | hSpCas9 | negative selection | 10017031 | 130433602 | 130433625 | 9 | - | 27869803 | HPAFII | viability after 35 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 0.7528527144093495 | [206] | [177,174,NaN] | 5 | hSpCas9 | negative selection | 10017032 | 130430571 | 130430594 | 9 | - | 27869803 | HPAFII | viability after 31 days | CCGTTCTGCCTGATGAAACCAGG | HMCN2 | ENSG00000148357 | 0.017434278121070568 | [665] | [362,394,NaN] | 0 | hSpCas9 | negative selection | 10099476 | 130430583 | 130430606 | 9 | + | 27869803 | HPAFII | viability after 31 days | AGGCAGAACGGAGTCTGCACAGG | HMCN2 | ENSG00000148357 | 0.012210478092623323 | [62] | [27,43,NaN] | 0 | hSpCas9 | negative selection | 10099477 | 130431406 | 130431429 | 9 | - | 27869803 | HPAFII | viability after 31 days | GCTGCCCTCAGTGTTGCGGCAGG | HMCN2 | ENSG00000148357 | -0.37141930812922774 | [166] | [115,24,NaN] | -3 | hSpCas9 | negative selection | 10099478 | 130432449 | 130432472 | 9 | + | 27869803 | HPAFII | viability after 31 days | GAGAGCATCGAGTGTGGACCCGG | HMCN2 | ENSG00000148357 | -5.947988513849402 | [109] | [0,0,NaN] | -9 | hSpCas9 | negative selection | 10099479 | 130432521 | 130432544 | 9 | + | 27869803 | HPAFII | viability after 31 days | TGTCCTGCCACCTACCGGCAGGG | HMCN2 | ENSG00000148357 | -0.6431152820471459 | [443] | [171,145,NaN] | -4 | hSpCas9 | negative selection | 10099480 | 130433602 | 130433625 | 9 | - | 27869803 | HPAFII | viability after 31 days | CACGGTGAGCCGGTAGAGGCCGG | HMCN2 | ENSG00000148357 | 1.4356531765964404 | [206] | [330,295,NaN] | 9 | hSpCas9 | negative selection | 10099481 |