Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:4750
• Symbol:NEK1
• Description:NIMA related kinase 1

DataSource: http://genomecrispr.dkfz.de/#!/results/NEK1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
169585473	169585496	4	+	26472758	Jiyoye	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.0029129018012371	[96]	[72]	0	hSpCas9	negative selection	107636
169585441	169585464	4	+	26472758	Jiyoye	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	0.5542796755380844	[176]	[195]	4	hSpCas9	negative selection	107637
169507739	169507762	4	-	26472758	Jiyoye	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.10996030539170665	[321]	[224]	-1	hSpCas9	negative selection	107638
169599155	169599178	4	-	26472758	Jiyoye	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	1.272015798224238	[122]	[223]	7	hSpCas9	negative selection	107639
169588706	169588729	4	-	26472758	Jiyoye	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.25178770065905975	[89]	[56]	-2	hSpCas9	negative selection	107640
169562149	169562172	4	-	26472758	Jiyoye	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	1.592390253972781	[72]	[165]	8	hSpCas9	negative selection	107641
169561871	169561894	4	-	26472758	Jiyoye	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.3495534674817627	[97]	[57]	-3	hSpCas9	negative selection	107642
169463335	169463358	4	-	26472758	Jiyoye	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	0.03794157184015423	[61]	[47]	0	hSpCas9	negative selection	107643
169424634	169424657	4	+	26472758	Jiyoye	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	5.7647273861239565	[0]	[40]	9	hSpCas9	negative selection	107644
169406664	169406687	4	+	26472758	Jiyoye	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.06393804217905918	[340]	[245]	0	hSpCas9	negative selection	107645
169585473	169585496	4	+	26472758	KBM7	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	0.33963232343932226	[413,404]	[233,253]	3	hSpCas9	negative selection	298754
169585441	169585464	4	+	26472758	KBM7	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.08195018827827627	[854,1003]	[440,397]	-1	hSpCas9	negative selection	298755
169507739	169507762	4	-	26472758	KBM7	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.3972679178604812	[1655,1428]	[629,476]	-4	hSpCas9	negative selection	298756
169599155	169599178	4	-	26472758	KBM7	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.6471392706487461	[696,515]	[166,191]	-5	hSpCas9	negative selection	298757
169588706	169588729	4	-	26472758	KBM7	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	0.381674910003964	[416,345]	[305,166]	4	hSpCas9	negative selection	298758
169562149	169562172	4	-	26472758	KBM7	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.011180013305253311	[930,689]	[472,296]	0	hSpCas9	negative selection	298759
169561871	169561894	4	-	26472758	KBM7	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.5812926794069407	[407,522]	[113,179]	-5	hSpCas9	negative selection	298760
169463335	169463358	4	-	26472758	KBM7	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	0.17393784491325226	[221,166]	[145,62]	1	hSpCas9	negative selection	298761
169424634	169424657	4	+	26472758	KBM7	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-0.4775310485939199	[59,152]	[51,24]	-4	hSpCas9	negative selection	298762
169406664	169406687	4	+	26472758	KBM7	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.0869079540145472	[1222,1046]	[643,372]	-1	hSpCas9	negative selection	298763
169585473	169585496	4	+	26472758	Raji	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.4775789653475566	[209]	[34]	-4	hSpCas9	negative selection	489872
169585441	169585464	4	+	26472758	Raji	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	0.7000077378028223	[312]	[117]	6	hSpCas9	negative selection	489873
169507739	169507762	4	-	26472758	Raji	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.7032917449756162	[869]	[123]	-5	hSpCas9	negative selection	489874
169599155	169599178	4	-	26472758	Raji	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-1.062541466068713	[422]	[46]	-7	hSpCas9	negative selection	489875
169588706	169588729	4	-	26472758	Raji	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	0.7973622071704115	[180]	[72]	7	hSpCas9	negative selection	489876
169562149	169562172	4	-	26472758	Raji	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.6445345561785673	[316]	[114]	6	hSpCas9	negative selection	489877
169561871	169561894	4	-	26472758	Raji	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.6923460360947558	[167]	[62]	6	hSpCas9	negative selection	489878
169463335	169463358	4	-	26472758	Raji	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-1.2911658411166753	[115]	[10]	-7	hSpCas9	negative selection	489879
169424634	169424657	4	+	26472758	Raji	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	3.844349129539806	[11]	[39]	9	hSpCas9	negative selection	489880
169406664	169406687	4	+	26472758	Raji	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.17619824786428007	[593]	[121]	-1	hSpCas9	negative selection	489881
169585473	169585496	4	+	26472758	K562	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	0.40510157680391057	[347]	[388]	3	hSpCas9	negative selection	680990
169585441	169585464	4	+	26472758	K562	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	0.04750321234582233	[579]	[505]	0	hSpCas9	negative selection	680991
169507739	169507762	4	-	26472758	K562	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.542520357837063	[1262]	[731]	-3	hSpCas9	negative selection	680992
169599155	169599178	4	-	26472758	K562	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.18588552826570748	[569]	[422]	-1	hSpCas9	negative selection	680993
169588706	169588729	4	-	26472758	K562	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.5367205419673584	[408]	[237]	-3	hSpCas9	negative selection	680994
169562149	169562172	4	-	26472758	K562	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.6685814631928929	[590]	[792]	5	hSpCas9	negative selection	680995
169561871	169561894	4	-	26472758	K562	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.92044570679406	[352]	[563]	7	hSpCas9	negative selection	680996
169463335	169463358	4	-	26472758	K562	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.04315786243013853	[165]	[135]	0	hSpCas9	negative selection	680997
169424634	169424657	4	+	26472758	K562	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	1.2800426373971685	[59]	[122]	9	hSpCas9	negative selection	680998
169406664	169406687	4	+	26472758	K562	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.27112305149550076	[1016]	[1035]	2	hSpCas9	negative selection	680999
169479473	169479496	4	-	26627737	DLD1	viability	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.8752250897265935	[662]	[337,415,386]	8	hSpCas9	negative selection	824419
169508797	169508820	4	-	26627737	DLD1	viability	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.638643024166007	[131]	[95,17,74]	6	hSpCas9	negative selection	824420
169537816	169537839	4	-	26627737	DLD1	viability	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-0.5915507034140873	[276]	[62,79,33]	-5	hSpCas9	negative selection	824421
169555931	169555954	4	-	26627737	DLD1	viability	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.432599675131333	[398]	[99,261,149]	4	hSpCas9	negative selection	824422
169576995	169577018	4	+	26627737	DLD1	viability	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	2.063203585172121	[16]	[21,41,5]	9	hSpCas9	negative selection	824423
169599153	169599176	4	-	26627737	DLD1	viability	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-1.5610352047325322	[165]	[14,20,16]	-8	hSpCas9	negative selection	824424
169479473	169479496	4	-	26627737	GBM cells	viability after 5 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.39880300195833085	[552]	[384,396]	6	hSpCas9	negative selection	906734
169508797	169508820	4	-	26627737	GBM cells	viability after 5 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.1832168974731303	[100]	[64,57]	3	hSpCas9	negative selection	906735
169537816	169537839	4	-	26627737	GBM cells	viability after 5 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.2747807768838518	[167]	[103,113]	4	hSpCas9	negative selection	906736
169555931	169555954	4	-	26627737	GBM cells	viability after 5 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.09685325249759447	[441]	[252,253]	1	hSpCas9	negative selection	906737
169576995	169577018	4	+	26627737	GBM cells	viability after 5 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.31395137416452923	[71]	[42,52]	5	hSpCas9	negative selection	906738
169599153	169599176	4	-	26627737	GBM cells	viability after 5 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	0.19264507794239583	[61]	[32,42]	3	hSpCas9	negative selection	906739
169479473	169479496	4	-	26627737	GBM cells	viability after 13 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.5960665361322242	[552]	[436,466]	7	hSpCas9	negative selection	989049
169508797	169508820	4	-	26627737	GBM cells	viability after 13 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.14718041092600753	[100]	[54,44]	-2	hSpCas9	negative selection	989050
169537816	169537839	4	-	26627737	GBM cells	viability after 13 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.5308811266608703	[167]	[150,115]	6	hSpCas9	negative selection	989051
169555931	169555954	4	-	26627737	GBM cells	viability after 13 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.44926011217491424	[441]	[445,230]	5	hSpCas9	negative selection	989052
169576995	169577018	4	+	26627737	GBM cells	viability after 13 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.42723164600701824	[71]	[78,30]	5	hSpCas9	negative selection	989053
169599153	169599176	4	-	26627737	GBM cells	viability after 13 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	0.6073869126970184	[61]	[69,35]	7	hSpCas9	negative selection	989054
169479473	169479496	4	-	26627737	GBM cells	viability after 21 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.9296974160818552	[552]	[635,513]	8	hSpCas9	negative selection	1071364
169508797	169508820	4	-	26627737	GBM cells	viability after 21 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.45851443524516544	[100]	[65,83]	5	hSpCas9	negative selection	1071365
169537816	169537839	4	-	26627737	GBM cells	viability after 21 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.5407857782626967	[167]	[137,127]	5	hSpCas9	negative selection	1071366
169555931	169555954	4	-	26627737	GBM cells	viability after 21 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.23647910673311734	[441]	[296,269]	2	hSpCas9	negative selection	1071367
169576995	169577018	4	+	26627737	GBM cells	viability after 21 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	1.403330544751955	[71]	[115,91]	9	hSpCas9	negative selection	1071368
169599153	169599176	4	-	26627737	GBM cells	viability after 21 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	0.5508527488662212	[61]	[52,45]	5	hSpCas9	negative selection	1071369
169479473	169479496	4	-	26627737	RPE1	viability after 9 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.511266268199849	[2275]	[812,1276]	3	hSpCas9	negative selection	1153679
169508797	169508820	4	-	26627737	RPE1	viability after 9 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.5432712967457028	[485]	[120,82]	-4	hSpCas9	negative selection	1153680
169537816	169537839	4	-	26627737	RPE1	viability after 9 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.20895261813455357	[379]	[78,212]	1	hSpCas9	negative selection	1153681
169555931	169555954	4	-	26627737	RPE1	viability after 9 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.794414476344109	[766]	[305,558]	5	hSpCas9	negative selection	1153682
169576995	169577018	4	+	26627737	RPE1	viability after 9 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	2.362501208689971	[41]	[33,110]	9	hSpCas9	negative selection	1153683
169599153	169599176	4	-	26627737	RPE1	viability after 9 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	1.9751525399970857	[121]	[140,161]	8	hSpCas9	negative selection	1153684
169479473	169479496	4	-	26627737	RPE1	viability after 12 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.5533420751574889	[2275]	[806,992]	3	hSpCas9	negative selection	1235994
169508797	169508820	4	-	26627737	RPE1	viability after 12 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.5483436344407978	[485]	[149,24]	-4	hSpCas9	negative selection	1235995
169537816	169537839	4	-	26627737	RPE1	viability after 12 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.8256461504655357	[379]	[144,218]	5	hSpCas9	negative selection	1235996
169555931	169555954	4	-	26627737	RPE1	viability after 12 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.4898073166535792	[766]	[182,401]	3	hSpCas9	negative selection	1235997
169576995	169577018	4	+	26627737	RPE1	viability after 12 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.603121215022135	[41]	[4,9]	-4	hSpCas9	negative selection	1235998
169599153	169599176	4	-	26627737	RPE1	viability after 12 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	2.0057029397694963	[121]	[203,54]	8	hSpCas9	negative selection	1235999
169479473	169479496	4	-	26627737	RPE1	viability after 15 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.3020768688734221	[2275]	[664,917]	2	hSpCas9	negative selection	1318309
169508797	169508820	4	-	26627737	RPE1	viability after 15 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.584299230362816	[485]	[136,47]	-4	hSpCas9	negative selection	1318310
169537816	169537839	4	-	26627737	RPE1	viability after 15 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	1.0977380572087618	[379]	[254,205]	6	hSpCas9	negative selection	1318311
169555931	169555954	4	-	26627737	RPE1	viability after 15 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.647432904473615	[766]	[319,358]	4	hSpCas9	negative selection	1318312
169576995	169577018	4	+	26627737	RPE1	viability after 15 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.0875918129695128	[41]	[0,20]	0	hSpCas9	negative selection	1318313
169599153	169599176	4	-	26627737	RPE1	viability after 15 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	2.1856530749612206	[121]	[184,129]	9	hSpCas9	negative selection	1318314
169479473	169479496	4	-	26627737	RPE1	viability after 18 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.43158862075146365	[2275]	[752,908]	2	hSpCas9	negative selection	1400624
169508797	169508820	4	-	26627737	RPE1	viability after 18 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.1351645698767312	[485]	[160,74]	-1	hSpCas9	negative selection	1400625
169537816	169537839	4	-	26627737	RPE1	viability after 18 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.9195845093930567	[379]	[105,287]	5	hSpCas9	negative selection	1400626
169555931	169555954	4	-	26627737	RPE1	viability after 18 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.5207664771440754	[766]	[266,328]	3	hSpCas9	negative selection	1400627
169576995	169577018	4	+	26627737	RPE1	viability after 18 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.9719314341107781	[41]	[28,14]	6	hSpCas9	negative selection	1400628
169599153	169599176	4	-	26627737	RPE1	viability after 18 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	2.2981054668552696	[121]	[217,101]	9	hSpCas9	negative selection	1400629
169479473	169479496	4	-	26627737	HeLa	viability after 8 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.806941174988805	[533]	[464,413,356]	6	hSpCas9	negative selection	1482939
169508797	169508820	4	-	26627737	HeLa	viability after 8 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.8014876368861998	[500]	[138,168,73]	-7	hSpCas9	negative selection	1482940
169537816	169537839	4	-	26627737	HeLa	viability after 8 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	1.5841175831423238	[122]	[131,61,272]	9	hSpCas9	negative selection	1482941
169555931	169555954	4	-	26627737	HeLa	viability after 8 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.7297296848109123	[402]	[380,349,169]	6	hSpCas9	negative selection	1482942
169576995	169577018	4	+	26627737	HeLa	viability after 8 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.14696442763271733	[71]	[16,45,19]	-1	hSpCas9	negative selection	1482943
169599153	169599176	4	-	26627737	HeLa	viability after 8 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.11639834655358161	[140]	[105,22,51]	-1	hSpCas9	negative selection	1482944
169479473	169479496	4	-	26627737	HeLa	viability after 12 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.8484769551539336	[533]	[471,295,436]	6	hSpCas9	negative selection	1565254
169508797	169508820	4	-	26627737	HeLa	viability after 12 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.455856765456739	[500]	[45,61,118]	-8	hSpCas9	negative selection	1565255
169537816	169537839	4	-	26627737	HeLa	viability after 12 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.678970856312219	[122]	[23,66,152]	5	hSpCas9	negative selection	1565256
169555931	169555954	4	-	26627737	HeLa	viability after 12 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.8249204061351845	[402]	[340,364,172]	6	hSpCas9	negative selection	1565257
169576995	169577018	4	+	26627737	HeLa	viability after 12 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.22913059600384766	[71]	[32,11,61]	2	hSpCas9	negative selection	1565258
169599153	169599176	4	-	26627737	HeLa	viability after 12 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-1.0896479190550215	[140]	[17,44,16]	-7	hSpCas9	negative selection	1565259
169479473	169479496	4	-	26627737	HeLa	viability after 15 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	1.0057554347032545	[533]	[289,505,521]	7	hSpCas9	negative selection	1647569
169508797	169508820	4	-	26627737	HeLa	viability after 15 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.0204925971352774	[500]	[84,89,128]	-7	hSpCas9	negative selection	1647570
169537816	169537839	4	-	26627737	HeLa	viability after 15 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.8444487650117035	[122]	[134,33,100]	6	hSpCas9	negative selection	1647571
169555931	169555954	4	-	26627737	HeLa	viability after 15 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.4089725164835663	[402]	[219,172,263]	3	hSpCas9	negative selection	1647572
169576995	169577018	4	+	26627737	HeLa	viability after 15 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.6106879216514067	[71]	[61,39,30]	5	hSpCas9	negative selection	1647573
169599153	169599176	4	-	26627737	HeLa	viability after 15 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.7684535970901318	[140]	[12,58,28]	-6	hSpCas9	negative selection	1647574
169479473	169479496	4	-	26627737	HeLa	viability after 18 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	1.0237122631205442	[533]	[201,422,521]	7	hSpCas9	negative selection	1729884
169508797	169508820	4	-	26627737	HeLa	viability after 18 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.0193871590924262	[500]	[55,75,128]	-7	hSpCas9	negative selection	1729885
169537816	169537839	4	-	26627737	HeLa	viability after 18 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.8468971795307694	[122]	[94,22,100]	6	hSpCas9	negative selection	1729886
169555931	169555954	4	-	26627737	HeLa	viability after 18 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.4735212409669032	[402]	[163,146,263]	4	hSpCas9	negative selection	1729887
169576995	169577018	4	+	26627737	HeLa	viability after 18 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.6515830258783442	[71]	[44,31,30]	5	hSpCas9	negative selection	1729888
169599153	169599176	4	-	26627737	HeLa	viability after 18 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.7252063476710975	[140]	[8,50,28]	-6	hSpCas9	negative selection	1729889
169479473	169479496	4	-	26627737	HCT116	viability after 6 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	-0.39532924576816725	[1116]	[264,60]	-2	hSpCas9	negative selection	1812199
169508797	169508820	4	-	26627737	HCT116	viability after 6 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.4091999858701656	[663]	[86,12]	-7	hSpCas9	negative selection	1812200
169537816	169537839	4	-	26627737	HCT116	viability after 6 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	1.7473746553580802	[237]	[269,45]	8	hSpCas9	negative selection	1812201
169555931	169555954	4	-	26627737	HCT116	viability after 6 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	1.9214808737633788	[552]	[532,216]	8	hSpCas9	negative selection	1812202
169576995	169577018	4	+	26627737	HCT116	viability after 6 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-4.375133862170886	[131]	[0,0]	-9	hSpCas9	negative selection	1812203
169599153	169599176	4	-	26627737	HCT116	viability after 6 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-1.2178118177563935	[355]	[54,6]	-6	hSpCas9	negative selection	1812204
169479473	169479496	4	-	26627737	HCT116	viability after 8 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.07353567079087753	[552]	[310,347,302]	0	hSpCas9	negative selection	1894514
169508797	169508820	4	-	26627737	HCT116	viability after 8 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.21685275524004954	[191]	[86,92,92]	-2	hSpCas9	negative selection	1894515
169537816	169537839	4	-	26627737	HCT116	viability after 8 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.3773654771433401	[177]	[129,102,148]	4	hSpCas9	negative selection	1894516
169555931	169555954	4	-	26627737	HCT116	viability after 8 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-0.21787613287141933	[432]	[210,235,168]	-2	hSpCas9	negative selection	1894517
169576995	169577018	4	+	26627737	HCT116	viability after 8 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.6611118571967247	[70]	[19,17,35]	-7	hSpCas9	negative selection	1894518
169599153	169599176	4	-	26627737	HCT116	viability after 8 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.09406201382485091	[136]	[105,51,54]	-1	hSpCas9	negative selection	1894519
169479473	169479496	4	-	26627737	HCT116	viability after 9 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.5411575205904936	[1116]	[576,320]	4	hSpCas9	negative selection	1976829
169508797	169508820	4	-	26627737	HCT116	viability after 9 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.3567278755984103	[663]	[116,313]	2	hSpCas9	negative selection	1976830
169537816	169537839	4	-	26627737	HCT116	viability after 9 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	2.111426125999361	[237]	[263,282]	9	hSpCas9	negative selection	1976831
169555931	169555954	4	-	26627737	HCT116	viability after 9 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.7846352856373074	[552]	[226,275]	5	hSpCas9	negative selection	1976832
169576995	169577018	4	+	26627737	HCT116	viability after 9 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.1479590685954908	[131]	[66,3]	-1	hSpCas9	negative selection	1976833
169599153	169599176	4	-	26627737	HCT116	viability after 9 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-1.4366411107051453	[355]	[33,35]	-7	hSpCas9	negative selection	1976834
169479473	169479496	4	-	26627737	HCT116	viability after 12 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.3188160451941099	[552]	[313,373,403]	3	hSpCas9	negative selection	2059144
169508797	169508820	4	-	26627737	HCT116	viability after 12 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.7749697445159675	[191]	[36,65,75]	-7	hSpCas9	negative selection	2059145
169537816	169537839	4	-	26627737	HCT116	viability after 12 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.39275757781191245	[177]	[85,94,189]	4	hSpCas9	negative selection	2059146
169555931	169555954	4	-	26627737	HCT116	viability after 12 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.15642278725193592	[432]	[176,353,238]	1	hSpCas9	negative selection	2059147
169576995	169577018	4	+	26627737	HCT116	viability after 12 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.7692979079719202	[70]	[15,13,35]	-7	hSpCas9	negative selection	2059148
169599153	169599176	4	-	26627737	HCT116	viability after 12 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.8627213222901378	[136]	[57,32,25]	-7	hSpCas9	negative selection	2059149
169479473	169479496	4	-	26627737	HCT116	viability after 15 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.3350964460089444	[552]	[364,470,361]	3	hSpCas9	negative selection	2141459
169508797	169508820	4	-	26627737	HCT116	viability after 15 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.5644150850822226	[191]	[107,64,54]	-5	hSpCas9	negative selection	2141460
169537816	169537839	4	-	26627737	HCT116	viability after 15 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.5716754298828137	[177]	[181,87,188]	5	hSpCas9	negative selection	2141461
169555931	169555954	4	-	26627737	HCT116	viability after 15 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.09951864203788052	[432]	[198,318,272]	1	hSpCas9	negative selection	2141462
169576995	169577018	4	+	26627737	HCT116	viability after 15 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-1.1117301001285047	[70]	[6,15,31]	-8	hSpCas9	negative selection	2141463
169599153	169599176	4	-	26627737	HCT116	viability after 15 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.9946392599870193	[136]	[43,34,39]	-7	hSpCas9	negative selection	2141464
169479473	169479496	4	-	26627737	HCT116	viability after 18 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.512360305349968	[552]	[402,483,243]	4	hSpCas9	negative selection	2223774
169508797	169508820	4	-	26627737	HCT116	viability after 18 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.3563195041232707	[191]	[55,8,45]	-8	hSpCas9	negative selection	2223775
169537816	169537839	4	-	26627737	HCT116	viability after 18 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-0.23840089090263805	[177]	[88,64,64]	-2	hSpCas9	negative selection	2223776
169555931	169555954	4	-	26627737	HCT116	viability after 18 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-0.45882467967310864	[432]	[92,175,187]	-4	hSpCas9	negative selection	2223777
169576995	169577018	4	+	26627737	HCT116	viability after 18 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-1.4612374374003458	[70]	[6,0,30]	-8	hSpCas9	negative selection	2223778
169599153	169599176	4	-	26627737	HCT116	viability after 18 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.5830794949208504	[136]	[63,32,35]	-5	hSpCas9	negative selection	2223779
169599171	169599194	4	-	24336569	HL60	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.3311539866442398	[745]	[1985]	3	hSpCas9	negative selection	2279737
169602007	169602030	4	-	24336569	HL60	viability	CAGTATAGAGAATCATTTGAAGG	NEK1	ENSG00000137601	-0.6029760109467234	[922]	[1285]	-5	hSpCas9	negative selection	2279738
169602019	169602042	4	+	24336569	HL60	viability	TCTCTATACTGGACAATATTTGG	NEK1	ENSG00000137601	0.057198679945879016	[1243]	[2738]	0	hSpCas9	negative selection	2279739
169602078	169602101	4	+	24336569	HL60	viability	TGATTCTTCTCTTTCTTTACTGG	NEK1	ENSG00000137601	-0.48705675412329114	[405]	[612]	-4	hSpCas9	negative selection	2279740
169602531	169602554	4	-	24336569	HL60	viability	TGGCAGACAGTATGTTATCAAGG	NEK1	ENSG00000137601	0.9406443115207471	[598]	[2432]	7	hSpCas9	negative selection	2279741
169602551	169602574	4	-	24336569	HL60	viability	CTTGTTAAATCTACAGAAGATGG	NEK1	ENSG00000137601	-1.0935622431467664	[478]	[474]	-7	hSpCas9	negative selection	2279742
169602556	169602579	4	+	24336569	HL60	viability	TTCTGTAGATTTAACAAGAATGG	NEK1	ENSG00000137601	-0.34391689184449664	[495]	[826]	-3	hSpCas9	negative selection	2279743
169602584	169602607	4	-	24336569	HL60	viability	AAGATTGGAGAAGGTTCATTTGG	NEK1	ENSG00000137601	-0.15227875135382574	[1356]	[2583]	-1	hSpCas9	negative selection	2279744
169602593	169602616	4	-	24336569	HL60	viability	AGACTACAGAAGATTGGAGAAGG	NEK1	ENSG00000137601	0.43989174594842706	[625]	[1796]	4	hSpCas9	negative selection	2279745
169602599	169602622	4	-	24336569	HL60	viability	TATGTTAGACTACAGAAGATTGG	NEK1	ENSG00000137601	0.372534389876581	[652]	[1788]	3	hSpCas9	negative selection	2279746
169599171	169599194	4	-	24336569	KBM7	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.07772168057415874	[372]	[963]	0	hSpCas9	negative selection	2346914
169602007	169602030	4	-	24336569	KBM7	viability	CAGTATAGAGAATCATTTGAAGG	NEK1	ENSG00000137601	-0.8844053845930131	[466]	[689]	-6	hSpCas9	negative selection	2346915
169602019	169602042	4	+	24336569	KBM7	viability	TCTCTATACTGGACAATATTTGG	NEK1	ENSG00000137601	0.5817099831329167	[706]	[2885]	5	hSpCas9	negative selection	2346916
169602078	169602101	4	+	24336569	KBM7	viability	TGATTCTTCTCTTTCTTTACTGG	NEK1	ENSG00000137601	0.766673380812334	[291]	[1354]	7	hSpCas9	negative selection	2346917
169602531	169602554	4	-	24336569	KBM7	viability	TGGCAGACAGTATGTTATCAAGG	NEK1	ENSG00000137601	-0.6245426522590087	[731]	[1294]	-5	hSpCas9	negative selection	2346918
169602551	169602574	4	-	24336569	KBM7	viability	CTTGTTAAATCTACAGAAGATGG	NEK1	ENSG00000137601	0.6678980208710535	[125]	[545]	6	hSpCas9	negative selection	2346919
169602556	169602579	4	+	24336569	KBM7	viability	TTCTGTAGATTTAACAAGAATGG	NEK1	ENSG00000137601	-0.2523519850012118	[313]	[718]	-2	hSpCas9	negative selection	2346920
169602584	169602607	4	-	24336569	KBM7	viability	AAGATTGGAGAAGGTTCATTTGG	NEK1	ENSG00000137601	0.9125499616275716	[722]	[3711]	7	hSpCas9	negative selection	2346921
169602593	169602616	4	-	24336569	KBM7	viability	AGACTACAGAAGATTGGAGAAGG	NEK1	ENSG00000137601	-0.4178318531548304	[575]	[1175]	-3	hSpCas9	negative selection	2346922
169602599	169602622	4	-	24336569	KBM7	viability	TATGTTAGACTACAGAAGATTGG	NEK1	ENSG00000137601	0.2415190455212728	[667]	[2153]	2	hSpCas9	negative selection	2346923
169520979	169521002	4	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCCTCTGTAGGCTCCACCTCTGG	NEK1	ENSG00000137601	0.22284639033454834	[76,251]	[35,48]	1	dCas9-VP64	positive selection	2384681
169447823	169447846	4	+	25494202	A375	resistance to PLX-4720 (puromycin)	CGTGCCACTGCACTCCAGCCCGG	NEK1	ENSG00000137601	0.4846974014178431	[92,26]	[27,6]	2	dCas9-VP64	positive selection	2391000
169579743	169579766	4	+	25494202	A375	resistance to PLX-4720 (puromycin)	TGCACCACTACACTCCAGCCCGG	NEK1	ENSG00000137601	1.5534549579245929	[63,822]	[25,576]	6	dCas9-VP64	positive selection	2395539
169532876	169532899	4	+	25494202	A375	resistance to PLX-4720 (puromycin)	TGTGCCACTGCACTCCAGTCAGG	NEK1	ENSG00000137601	0.7065915118721766	[198,108]	[43,65]	3	dCas9-VP64	positive selection	2424083
169576030	169576053	4	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCGGAGGTTGCAGTGAGCCAAAG	NEK1	ENSG00000137601	0.7460875795897541	[229,125]	[97,29]	3	dCas9-VP64	positive selection	2438690
169409335	169409358	4	+	25494202	A375	resistance to PLX-4720 (puromycin)	GGGGTTTCACCATGTTAGCCAGG	NEK1	ENSG00000137601	-0.6909980618209312	[118,65]	[15,7]	-4	dCas9-VP64	positive selection	2439268
169409691	169409714	4	-	25494202	A375	resistance to PLX-4720 (puromycin)	TCACTACAACCTCCGCCTCCTAG	NEK1	ENSG00000137601	-1.3762222380081446	[352,174]	[18,24]	-7	dCas9-VP64	positive selection	2454214
169607609	169607632	4	-	25494202	A375	resistance to PLX-4720 (puromycin)	AATTAGCCGGGCGTGGTGGCAGG	NEK1	ENSG00000137601	0.43464799396045883	[0,13]	[2,0]	2	dCas9-VP64	positive selection	2460917
169570096	169570119	4	+	25494202	A375	resistance to PLX-4720 (puromycin)	TGCCTGGCCGCCCATCGTCTGAG	NEK1	ENSG00000137601	-0.0329650900813252	[388,64]	[87,4]	0	dCas9-VP64	positive selection	2468138
169520979	169521002	4	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCCTCTGTAGGCTCCACCTCTGG	NEK1	ENSG00000137601	-0.3137172453886481	[73,192]	[24,43]	-3	dCas9-VP64	positive selection	2479936
169447823	169447846	4	+	25494202	A375	resistance to PLX-4720 (zeocin)	CGTGCCACTGCACTCCAGCCCGG	NEK1	ENSG00000137601	0.6971910689480432	[49,47]	[24,25]	5	dCas9-VP64	positive selection	2486255
169579743	169579766	4	+	25494202	A375	resistance to PLX-4720 (zeocin)	TGCACCACTACACTCCAGCCCGG	NEK1	ENSG00000137601	-0.4021395541078192	[270,438]	[81,91]	-4	dCas9-VP64	positive selection	2490794
169532876	169532899	4	+	25494202	A375	resistance to PLX-4720 (zeocin)	TGTGCCACTGCACTCCAGTCAGG	NEK1	ENSG00000137601	-0.12403431172340174	[312,117]	[32,93]	-1	dCas9-VP64	positive selection	2519338
169576030	169576053	4	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCGGAGGTTGCAGTGAGCCAAAG	NEK1	ENSG00000137601	4.721891124701559	[222,102]	[98,2655]	9	dCas9-VP64	positive selection	2533945
169409335	169409358	4	+	25494202	A375	resistance to PLX-4720 (zeocin)	GGGGTTTCACCATGTTAGCCAGG	NEK1	ENSG00000137601	0.84274483203793	[27,27]	[10,20]	5	dCas9-VP64	positive selection	2534523
169409691	169409714	4	-	25494202	A375	resistance to PLX-4720 (zeocin)	TCACTACAACCTCCGCCTCCTAG	NEK1	ENSG00000137601	-1.0610924962734893	[123,419]	[26,55]	-8	dCas9-VP64	positive selection	2549469
169607609	169607632	4	-	25494202	A375	resistance to PLX-4720 (zeocin)	AATTAGCCGGGCGTGGTGGCAGG	NEK1	ENSG00000137601	-0.5732050346504929	[30,53]	[10,6]	-5	dCas9-VP64	positive selection	2556172
169570096	169570119	4	+	25494202	A375	resistance to PLX-4720 (zeocin)	TGCCTGGCCGCCCATCGTCTGAG	NEK1	ENSG00000137601	-0.3398026399627511	[257,143]	[72,31]	-3	dCas9-VP64	positive selection	2563393
169507072	169507095	4	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	-0.5092562805807179	[2,2]	[2,0]	-6	hSpCas9	positive selection	3005228
169588705	169588728	4	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.3269425658589861	[3,3]	[4,2]	7	hSpCas9	positive selection	3005229
169585442	169585465	4	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.1776001203379313	[3,3]	[4,0]	-3	hSpCas9	positive selection	3005230
169556003	169556026	4	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	-0.6690772482393457	[3,2]	[0,1]	-7	hSpCas9	positive selection	3005231
169507072	169507095	4	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	0.07181474863976954	[2,2]	[3,1]	1	hSpCas9	positive selection	3073086
169588705	169588728	4	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.41510620357423	[3,3]	[2,0]	-6	hSpCas9	positive selection	3073087
169585442	169585465	4	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	0.346295923266793	[3,3]	[2,2]	6	hSpCas9	positive selection	3073088
169556003	169556026	4	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	-0.316793226352868	[3,3]	[2,0]	-5	hSpCas9	positive selection	3073089
169590725	169590748		-	24336571	A375	resistance to PLX after 7 days	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.013698047700003713	[88,84]	[87,82]	0	hSpCas9	positive selection	3130294
169599171	169599194		-	24336571	A375	resistance to PLX after 7 days	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.2929407705851922	[20,21]	[25,25]	7	hSpCas9	positive selection	3130295
169590725	169590748		-	24336571	A375	resistance to PLX after 14 days	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.22056161119012208	[102,83]	[51,48]	2	hSpCas9	positive selection	3188087
169599171	169599194		-	24336571	A375	resistance to PLX after 14 days	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.23750941260540703	[18,15]	[9,8]	2	hSpCas9	positive selection	3188088
169590725	169590748		-	24336571	A375	viability after 7 days	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.21502560126298675	[78]	[88,84]	3	hSpCas9	negative selection	3245880
169599171	169599194		-	24336571	A375	viability after 7 days	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.5906574041615255	[33]	[20,21]	-7	hSpCas9	negative selection	3245881
169590725	169590748		-	24336571	A375	viability after 14 days	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.3393330770409242	[78]	[102,83]	4	hSpCas9	negative selection	3303673
169599171	169599194		-	24336571	A375	viability after 14 days	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.834892358136295	[33]	[18,15]	-8	hSpCas9	negative selection	3303674
169599122	169599145	4	-	27383988	293T	resistance to West Nile virus (flavivirus)	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	3.00939869800225	[18,18]	[0,0]	8	hSpCas9	positive selection	3381436
169590755	169590778	4	+	27383988	293T	resistance to West Nile virus (flavivirus)	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	-0.9423461333905293	[293,293]	[0,0]	-6	hSpCas9	positive selection	3381437
169507072	169507095	4	-	26780180	HT29	viability (Avana library 4 designs)	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	2.40662418657528	[]	[]	8	hSpCas9	negative selection	3443062
169588705	169588728	4	-	26780180	HT29	viability (Avana library 4 designs)	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.480584690763	[]	[]	-7	hSpCas9	negative selection	3443063
169585441	169585464	4	+	26780180	HT29	viability (Avana library 4 designs)	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	1.88699704530478	[]	[]	7	hSpCas9	negative selection	3443064
169556002	169556025	4	+	26780180	HT29	viability (Avana library 4 designs)	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	0.625406768482007	[]	[]	2	hSpCas9	negative selection	3443065
169585441	169585464	4	+	26780180	A375	viability (Avana lentiCRISPRv2)	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-6.45440062427865	[]	[]	-6	hSpCas9	negative selection	3534569
169507072	169507095	4	-	26780180	A375	viability (Avana lentiCRISPRv2)	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	-2.86173811087945	[]	[]	-3	hSpCas9	negative selection	3534570
169588705	169588728	4	-	26780180	A375	viability (Avana lentiCRISPRv2)	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	6.46023965033248	[]	[]	9	hSpCas9	negative selection	3534571
169556002	169556025	4	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	-2.99964021798398	[]	[]	-4	hSpCas9	negative selection	3534572
169507045	169507068	4	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGAGAGAGAAAAAAAAGTGTGGG	NEK1	ENSG00000137601	-7.30539168032225	[]	[]	-6	hSpCas9	negative selection	3534573
169562149	169562172	4	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	-4.22121596223641	[]	[]	-5	hSpCas9	negative selection	3534574
169585441	169585464	4	+	26780180	A375	viability (Avana lentiGuide)	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	21.1222868022833	[]	[]	9	hSpCas9	negative selection	3643228
169507072	169507095	4	-	26780180	A375	viability (Avana lentiGuide)	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	4.30355894880589	[]	[]	4	hSpCas9	negative selection	3643229
169588705	169588728	4	-	26780180	A375	viability (Avana lentiGuide)	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-4.6198026284965	[]	[]	-7	hSpCas9	negative selection	3643230
169556002	169556025	4	+	26780180	A375	viability (Avana lentiGuide)	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	5.85604850448009	[]	[]	5	hSpCas9	negative selection	3643231
169507045	169507068	4	-	26780180	A375	viability (Avana lentiGuide)	TGAGAGAGAAAAAAAAGTGTGGG	NEK1	ENSG00000137601	0.698626257270264	[]	[]	0	hSpCas9	negative selection	3643232
169562149	169562172	4	-	26780180	A375	viability (Avana lentiGuide)	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.929707016878345	[]	[]	1	hSpCas9	negative selection	3643233
169585441	169585464	4	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.05990727492588678	[5]	[5,4]	-1	hSpCas9	positive selection	3751887
169507072	169507095	4	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	-0.42419608360304756	[5]	[4,2]	-6	hSpCas9	positive selection	3751888
169588705	169588728	4	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.3034583129941259	[4]	[5,5]	8	hSpCas9	positive selection	3751889
169556002	169556025	4	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	-0.4736191087000252	[4]	[4,1]	-6	hSpCas9	positive selection	3751890
169507045	169507068	4	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGAGAGAGAAAAAAAAGTGTGGG	NEK1	ENSG00000137601	-0.7456628028586105	[7]	[4,3]	-8	hSpCas9	positive selection	3751891
169562149	169562172	4	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	-0.6156259287014536	[5]	[3,2]	-7	hSpCas9	positive selection	3751892
169585441	169585464	4	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.36845659078916926	[4]	[0,5]	-5	hSpCas9	positive selection	3860494
169507072	169507095	4	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ACTAGAACGAAAGAGAAAGGAGG	NEK1	ENSG00000137601	-0.48502222420083785	[4]	[2,2]	-6	hSpCas9	positive selection	3860495
169588705	169588728	4	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.38602076060169077	[4]	[6,4]	9	hSpCas9	positive selection	3860496
169556002	169556025	4	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCATTTGGTCAAAAATGGCATGG	NEK1	ENSG00000137601	-0.5338435113822697	[4]	[3,1]	-6	hSpCas9	positive selection	3860497
169507045	169507068	4	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGAGAGAGAAAAAAAAGTGTGGG	NEK1	ENSG00000137601	0.12966046818778276	[5]	[5,5]	3	hSpCas9	positive selection	3860498
169562149	169562172	4	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	-0.029460264286592944	[5]	[3,4]	0	hSpCas9	positive selection	3860499
169599122	169599145	4	-	26780180	A375	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.992912950170551	[]	[]	4	hSpCas9	negative selection	3971821
169589468	169589491	4	-	26780180	A375	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	1.08413102144353	[]	[]	5	hSpCas9	negative selection	3971822
169590725	169590748	4	-	26780180	A375	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	1.53770439437163	[]	[]	8	hSpCas9	negative selection	3971823
169599171	169599194	4	-	26780180	A375	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.641220450551662	[]	[]	2	hSpCas9	negative selection	3971824
169590755	169590778	4	+	26780180	A375	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.818044065858621	[]	[]	3	hSpCas9	negative selection	3971825
169599122	169599145	4	-	26780180	HT29	viability (GeCKOv2 library)	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.789452004625055	[]	[]	3	hSpCas9	negative selection	4079787
169589468	169589491	4	-	26780180	HT29	viability (GeCKOv2 library)	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	1.06556292609056	[]	[]	5	hSpCas9	negative selection	4079788
169590725	169590748	4	-	26780180	HT29	viability (GeCKOv2 library)	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	1.04339248219288	[]	[]	5	hSpCas9	negative selection	4079789
169599171	169599194	4	-	26780180	HT29	viability (GeCKOv2 library)	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.918226425677724	[]	[]	4	hSpCas9	negative selection	4079790
169590755	169590778	4	+	26780180	HT29	viability (GeCKOv2 library)	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.658840749624397	[]	[]	2	hSpCas9	negative selection	4079791
169590725	169590748	4	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.4627924289185191	[6]	[6,5]	8	hSpCas9	positive selection	4154933
169599171	169599194	4	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.026001613704136728	[4]	[3,3]	0	hSpCas9	positive selection	4154934
169590725	169590748	4	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.9074242120617089	[5]	[5,5,4,3]	6	hSpCas9	positive selection	4218955
169599171	169599194	4	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.2228309758819961	[4]	[3,1,4,0]	1	hSpCas9	positive selection	4218956
169590725	169590748	4	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.027393616844089197	[3]	[3,1]	0	hSpCas9	positive selection	4285293
169599171	169599194	4	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.37773033076374163	[0]	[0,0]	-7	hSpCas9	positive selection	4285294
169589468	169589491	4	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	-0.08090016257586996	[1]	[1,0]	-1	hSpCas9	positive selection	4285295
169599122	169599145	4	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.20294938807572643	[1]	[2,1]	4	hSpCas9	positive selection	4349552
169590755	169590778	4	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.2616764111404044	[3]	[3,2]	5	hSpCas9	positive selection	4349553
169508247	169508269	4	-	27760321	OCIAML3	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.2805703490982605	[386,309]	[260,417]	3	hSpCas9	negative selection	4421867
169537808	169537830	4	-	27760321	OCIAML3	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-1.011087012025633	[166,159]	[76,45]	-7	hSpCas9	negative selection	4421868
169576996	169577018	4	+	27760321	OCIAML3	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.035467247265702384	[552,444]	[405,342]	0	hSpCas9	negative selection	4421869
169577050	169577072	4	+	27760321	OCIAML3	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	0.034322471592432735	[364,308]	[294,234]	0	hSpCas9	negative selection	4421870
169588705	169588727	4	-	27760321	OCIAML3	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.23270162435968422	[356,333]	[308,328]	2	hSpCas9	negative selection	4421871
169508247	169508269	4	-	27760321	OCIAML2	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.0035076470126250148	[386,309]	[242,330]	0	hSpCas9	negative selection	4505330
169537808	169537830	4	-	27760321	OCIAML2	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-1.3397587165672713	[166,159]	[44,61]	-8	hSpCas9	negative selection	4505331
169576996	169577018	4	+	27760321	OCIAML2	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.46795873319083325	[552,444]	[237,358]	-5	hSpCas9	negative selection	4505332
169577050	169577072	4	+	27760321	OCIAML2	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.19477594684834787	[364,308]	[248,221]	-2	hSpCas9	negative selection	4505333
169588705	169588727	4	-	27760321	OCIAML2	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.09401434744023507	[356,333]	[239,290]	-1	hSpCas9	negative selection	4505334
169508247	169508269	4	-	27760321	MV411	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	1.105382246186052	[386,309]	[664,231]	7	hSpCas9	negative selection	4588793
169537808	169537830	4	-	27760321	MV411	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-0.22282718347650876	[166,159]	[84,103]	-2	hSpCas9	negative selection	4588794
169576996	169577018	4	+	27760321	MV411	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.3277243273510212	[552,444]	[148,428]	-2	hSpCas9	negative selection	4588795
169577050	169577072	4	+	27760321	MV411	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	0.2566330417859746	[364,308]	[195,367]	1	hSpCas9	negative selection	4588796
169588705	169588727	4	-	27760321	MV411	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.19421190490129508	[356,333]	[186,217]	-1	hSpCas9	negative selection	4588797
169508247	169508269	4	-	27760321	HL60	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.2711433015253076	[386,309]	[243,444]	3	hSpCas9	negative selection	4672256
169537808	169537830	4	-	27760321	HL60	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-0.05259298256641287	[166,159]	[94,164]	0	hSpCas9	negative selection	4672257
169576996	169577018	4	+	27760321	HL60	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.016919945076642712	[552,444]	[380,440]	0	hSpCas9	negative selection	4672258
169577050	169577072	4	+	27760321	HL60	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	0.5728729047818449	[364,308]	[458,353]	6	hSpCas9	negative selection	4672259
169588705	169588727	4	-	27760321	HL60	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.257455854260232	[356,333]	[355,317]	3	hSpCas9	negative selection	4672260
169508247	169508269	4	-	27760321	HT1080	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	1.5460927497736614	[309,241]	[480,145]	8	hSpCas9	negative selection	4755719
169537808	169537830	4	-	27760321	HT1080	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-0.5945906768230367	[159,124]	[7,69]	-3	hSpCas9	negative selection	4755720
169576996	169577018	4	+	27760321	HT1080	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-1.89924347099366	[444,346]	[79,0]	-6	hSpCas9	negative selection	4755721
169577050	169577072	4	+	27760321	HT1080	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	0.977311260976682	[308,240]	[250,176]	5	hSpCas9	negative selection	4755722
169588705	169588727	4	-	27760321	HT1080	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-4.484091449174417	[333,260]	[0,9]	-8	hSpCas9	negative selection	4755723
169508247	169508269	4	-	27760321	HT29	viability after 7 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.23314482351280744	[309,241]	[399,390,455]	3	hSpCas9	negative selection	4839182
169537808	169537830	4	-	27760321	HT29	viability after 7 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.1447278569027719	[159,124]	[127,273,194]	2	hSpCas9	negative selection	4839183
169576996	169577018	4	+	27760321	HT29	viability after 7 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.38859818507635324	[444,346]	[283,440,424]	-5	hSpCas9	negative selection	4839184
169577050	169577072	4	+	27760321	HT29	viability after 7 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.8707503641207641	[308,240]	[407,60,142]	-8	hSpCas9	negative selection	4839185
169588705	169588727	4	-	27760321	HT29	viability after 7 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.5405770664893448	[333,260]	[470,493,686]	7	hSpCas9	negative selection	4839186
169508247	169508269	4	-	27760321	HT29	viability after 25 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.870756518096695	[309,241]	[485,613,641]	8	hSpCas9	negative selection	4922645
169537808	169537830	4	-	27760321	HT29	viability after 25 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.40003100756069776	[159,124]	[219,157,271]	4	hSpCas9	negative selection	4922646
169576996	169577018	4	+	27760321	HT29	viability after 25 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.7632786094881436	[444,346]	[327,179,289]	-6	hSpCas9	negative selection	4922647
169577050	169577072	4	+	27760321	HT29	viability after 25 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.39551559610208553	[308,240]	[262,202,249]	-4	hSpCas9	negative selection	4922648
169588705	169588727	4	-	27760321	HT29	viability after 25 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.3399905762866037	[333,260]	[379,462,452]	3	hSpCas9	negative selection	4922649
169508247	169508269	4	-	27760321	HT29	viability after 22 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.6590670410399417	[309,241]	[320,318,879]	7	hSpCas9	negative selection	5006108
169537808	169537830	4	-	27760321	HT29	viability after 22 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.2584388895469478	[159,124]	[214,252,121]	2	hSpCas9	negative selection	5006109
169576996	169577018	4	+	27760321	HT29	viability after 22 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.5460265608738492	[444,346]	[184,345,413]	-5	hSpCas9	negative selection	5006110
169577050	169577072	4	+	27760321	HT29	viability after 22 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.20687550627360096	[308,240]	[210,239,377]	-2	hSpCas9	negative selection	5006111
169588705	169588727	4	-	27760321	HT29	viability after 22 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.23862199631997916	[333,260]	[353,545,317]	2	hSpCas9	negative selection	5006112
169508247	169508269	4	-	27760321	HT29	viability after 19 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.990184816184319	[309,241]	[703,510,671]	8	hSpCas9	negative selection	5089571
169537808	169537830	4	-	27760321	HT29	viability after 19 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	-0.08016621055289885	[159,124]	[150,234,78]	0	hSpCas9	negative selection	5089572
169576996	169577018	4	+	27760321	HT29	viability after 19 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-1.0499614591933126	[444,346]	[401,128,146]	-6	hSpCas9	negative selection	5089573
169577050	169577072	4	+	27760321	HT29	viability after 19 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.02648286002648831	[308,240]	[426,108,398]	0	hSpCas9	negative selection	5089574
169588705	169588727	4	-	27760321	HT29	viability after 19 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.24211546773936404	[333,260]	[257,265,333]	-2	hSpCas9	negative selection	5089575
169508247	169508269	4	-	27760321	HT29	viability after 16 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.608649174255909	[309,241]	[507,432,475]	7	hSpCas9	negative selection	5173034
169537808	169537830	4	-	27760321	HT29	viability after 16 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.6892511225392122	[159,124]	[290,349,130]	7	hSpCas9	negative selection	5173035
169576996	169577018	4	+	27760321	HT29	viability after 16 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.43291696055259554	[444,346]	[334,255,395]	-4	hSpCas9	negative selection	5173036
169577050	169577072	4	+	27760321	HT29	viability after 16 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.13326669218552178	[308,240]	[385,297,166]	-1	hSpCas9	negative selection	5173037
169588705	169588727	4	-	27760321	HT29	viability after 16 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.006467715535024843	[333,260]	[261,303,430]	0	hSpCas9	negative selection	5173038
169508247	169508269	4	-	27760321	HT29	viability after 13 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.9898764543611502	[309,241]	[751,632,528]	9	hSpCas9	negative selection	5256497
169537808	169537830	4	-	27760321	HT29	viability after 13 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.6466215422538619	[159,124]	[209,332,234]	8	hSpCas9	negative selection	5256498
169576996	169577018	4	+	27760321	HT29	viability after 13 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.3780556561297207	[444,346]	[440,296,323]	-4	hSpCas9	negative selection	5256499
169577050	169577072	4	+	27760321	HT29	viability after 13 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	0.5320241046420379	[308,240]	[471,471,440]	7	hSpCas9	negative selection	5256500
169588705	169588727	4	-	27760321	HT29	viability after 13 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.4808924912049073	[333,260]	[239,798,410]	6	hSpCas9	negative selection	5256501
169508247	169508269	4	-	27760321	HT29	viability after 10 days	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	0.5525743143439046	[309,241]	[527,510,477]	7	hSpCas9	negative selection	5339960
169537808	169537830	4	-	27760321	HT29	viability after 10 days	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.18559664559058908	[159,124]	[203,280,125]	2	hSpCas9	negative selection	5339961
169576996	169577018	4	+	27760321	HT29	viability after 10 days	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.6701504159465603	[444,346]	[369,242,316]	-7	hSpCas9	negative selection	5339962
169577050	169577072	4	+	27760321	HT29	viability after 10 days	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-0.23747885652776868	[308,240]	[258,352,263]	-3	hSpCas9	negative selection	5339963
169588705	169588727	4	-	27760321	HT29	viability after 10 days	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	-0.018130033705553106	[333,260]	[321,377,399]	0	hSpCas9	negative selection	5339964
169508247	169508269	4	-	27760321	MOLM13	viability	GCCAAACTTCGTGGTGAAAAGG	NEK1	ENSG00000137601	1.0620050894289452	[386,309]	[412,403]	7	hSpCas9	negative selection	5423423
169537808	169537830	4	-	27760321	MOLM13	viability	GCTCGAGCCGAAGGACATATGG	NEK1	ENSG00000137601	0.20242891640388283	[166,159]	[229,0]	1	hSpCas9	negative selection	5423424
169576996	169577018	4	+	27760321	MOLM13	viability	GTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.32272203716811154	[552,444]	[354,345]	2	hSpCas9	negative selection	5423425
169577050	169577072	4	+	27760321	MOLM13	viability	TCGAGTTTTGTCCTGAAGCTGG	NEK1	ENSG00000137601	-2.012526430147008	[364,308]	[60,34]	-8	hSpCas9	negative selection	5423426
169588705	169588727	4	-	27760321	MOLM13	viability	GCTGGCTCGAACTTGCATAGGG	NEK1	ENSG00000137601	0.10339771542306775	[356,333]	[114,289]	0	hSpCas9	negative selection	5423427
169612538	169612561	4	+	27661255	K562	viability	GGTAAGGACTCCGCAACCTAGGG	NEK1	ENSG00000137601	0.190934839141653	[2419,2942]	[2659,2245]	6	dCas9-KRAB	negative selection	5496186
169612259	169612282	4	-	27661255	K562	viability	GGGCGTCGCCCGAGACAGGACGG	NEK1	ENSG00000137601	-0.0031633536207806845	[1930,1919]	[1779,1325]	0	dCas9-KRAB	negative selection	5496187
169612529	169612552	4	-	27661255	K562	viability	GCGGAGTCCTTACCCTACCCTGG	NEK1	ENSG00000137601	0.2730729607602997	[1086,1043]	[1174,904]	8	dCas9-KRAB	negative selection	5496188
169590725	169590748	4	-	27260156	BXPC3	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.10586765944428644	[725]	[788,713,873,905]	2	hSpCas9	negative selection	5557753
169599171	169599194	4	-	27260156	BXPC3	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.11148510140258905	[50]	[19,48,48,86]	-3	hSpCas9	negative selection	5557754
169589468	169589491	4	-	27260156	BXPC3	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.46256728958564497	[141]	[185,230,188,211]	8	hSpCas9	negative selection	5557755
169599122	169599145	4	-	27260156	BXPC3	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.034570044237927755	[175]	[124,182,200,259]	0	hSpCas9	negative selection	5622012
169590755	169590778	4	+	27260156	BXPC3	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.2479087624154815	[623]	[727,667,812,913]	5	hSpCas9	negative selection	5622013
169590725	169590748	4	-	27260156	A673	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.1440300727544025	[725]	[1048,931,949,928]	3	hSpCas9	negative selection	5679004
169599171	169599194	4	-	27260156	A673	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.04376271148919164	[50]	[52,80,50,65]	1	hSpCas9	negative selection	5679005
169589468	169589491	4	-	27260156	A673	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.27797001301770696	[141]	[242,184,128,278]	6	hSpCas9	negative selection	5679006
169599122	169599145	4	-	27260156	A673	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.5831449587797022	[175]	[109,167,135,145]	-8	hSpCas9	negative selection	5743263
169590755	169590778	4	+	27260156	A673	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.1042662794099713	[623]	[779,823,771,842]	2	hSpCas9	negative selection	5743264
169590725	169590748	4	-	27260156	A375	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.10092999720383933	[725]	[759,926,974,545]	-1	hSpCas9	negative selection	5800255
169599171	169599194	4	-	27260156	A375	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-1.2348634347854022	[50]	[26,43,18,14]	-8	hSpCas9	negative selection	5800256
169589468	169589491	4	-	27260156	A375	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.40000188243896995	[141]	[240,217,135,272]	6	hSpCas9	negative selection	5800257
169599122	169599145	4	-	27260156	A375	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-1.3241420884868575	[175]	[96,74,61,91]	-9	hSpCas9	negative selection	5864514
169590755	169590778	4	+	27260156	A375	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.415020111389757	[623]	[1002,1249,1000,700]	6	hSpCas9	negative selection	5864515
169590725	169590748	4	-	27260156	COLO741	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.1632414277920129	[725]	[1054,1045,1015]	3	hSpCas9	negative selection	5921506
169599171	169599194	4	-	27260156	COLO741	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.18026082897876128	[50]	[67,80,71]	4	hSpCas9	negative selection	5921507
169589468	169589491	4	-	27260156	COLO741	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.08312994576539401	[141]	[194,141,235]	1	hSpCas9	negative selection	5921508
169599122	169599145	4	-	27260156	COLO741	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.8540570185198875	[175]	[44,135,180]	-9	hSpCas9	negative selection	5985765
169590755	169590778	4	+	27260156	COLO741	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.4100361751439257	[623]	[1186,1102,907]	8	hSpCas9	negative selection	5985766
169590725	169590748	4	-	27260156	CAL120	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.11942709437817178	[725]	[722,814,1359]	1	hSpCas9	negative selection	6042757
169599171	169599194	4	-	27260156	CAL120	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.08825664281114864	[50]	[127,44,38]	1	hSpCas9	negative selection	6042758
169589468	169589491	4	-	27260156	CAL120	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.6697372667019601	[141]	[313,357,177]	8	hSpCas9	negative selection	6042759
169599122	169599145	4	-	27260156	CAL120	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.005619342188112997	[175]	[212,198,245]	0	hSpCas9	negative selection	6107016
169590755	169590778	4	+	27260156	CAL120	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.688577352357326	[623]	[1720,988,1116]	8	hSpCas9	negative selection	6107017
169590725	169590748	4	-	27260156	CADOES1	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.24034980887878865	[725]	[832,737,726,895]	4	hSpCas9	negative selection	6164008
169599171	169599194	4	-	27260156	CADOES1	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.1680664528634721	[50]	[22,50,50,43]	-3	hSpCas9	negative selection	6164009
169589468	169589491	4	-	27260156	CADOES1	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.1455156951129455	[141]	[176,154,87,164]	2	hSpCas9	negative selection	6164010
169599122	169599145	4	-	27260156	CADOES1	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.21988424990301025	[175]	[149,104,168,137]	-4	hSpCas9	negative selection	6228267
169590755	169590778	4	+	27260156	CADOES1	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.3552927033822018	[623]	[711,654,771,833]	6	hSpCas9	negative selection	6228268
169590725	169590748	4	-	27260156	EWS502	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.25962926043229506	[725]	[945,637,862,1263]	4	hSpCas9	negative selection	6285259
169599171	169599194	4	-	27260156	EWS502	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.5025109445255369	[50]	[36,39,27,47]	-7	hSpCas9	negative selection	6285260
169589468	169589491	4	-	27260156	EWS502	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.1860592488842272	[141]	[143,142,134,272]	3	hSpCas9	negative selection	6285261
169599122	169599145	4	-	27260156	EWS502	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.2549155207875997	[175]	[127,198,154,142]	-4	hSpCas9	negative selection	6349518
169590755	169590778	4	+	27260156	EWS502	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.27549794149946333	[623]	[637,759,914,914]	5	hSpCas9	negative selection	6349519
169590725	169590748	4	-	27260156	EW8	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.08919257788476231	[725]	[712,685,305,565]	-2	hSpCas9	negative selection	6406510
169599171	169599194	4	-	27260156	EW8	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.35456937907098807	[50]	[155,28,26,14]	6	hSpCas9	negative selection	6406511
169589468	169589491	4	-	27260156	EW8	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	-0.019432979953801555	[141]	[71,105,131,135]	0	hSpCas9	negative selection	6406512
169599122	169599145	4	-	27260156	EW8	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-1.1368615988848125	[175]	[72,87,66,31]	-9	hSpCas9	negative selection	6470769
169590755	169590778	4	+	27260156	EW8	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.27832784881866196	[623]	[1035,478,445,564]	5	hSpCas9	negative selection	6470770
169590725	169590748	4	-	27260156	CORL105	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.34152284741597483	[725]	[566,745,722,649]	-6	hSpCas9	negative selection	6527761
169599171	169599194	4	-	27260156	CORL105	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.013069757708765284	[50]	[97,55,57,25]	0	hSpCas9	negative selection	6527762
169589468	169589491	4	-	27260156	CORL105	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.27659798006564196	[141]	[214,184,218,189]	5	hSpCas9	negative selection	6527763
169599122	169599145	4	-	27260156	CORL105	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.9614470568895426	[175]	[82,89,145,109]	-9	hSpCas9	negative selection	6592020
169590755	169590778	4	+	27260156	CORL105	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.3407277578991524	[623]	[844,870,1125,887]	6	hSpCas9	negative selection	6592021
169590725	169590748	4	-	27260156	HS294T	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.5385105414172003	[725]	[152,157,215,261]	-6	hSpCas9	negative selection	6649012
169599171	169599194	4	-	27260156	HS294T	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.3023767343954731	[50]	[9,10,15,29]	-4	hSpCas9	negative selection	6649013
169589468	169589491	4	-	27260156	HS294T	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.45488918910543763	[141]	[44,65,107,86]	6	hSpCas9	negative selection	6649014
169599122	169599145	4	-	27260156	HS294T	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-1.1119293120797065	[175]	[14,41,43,24]	-9	hSpCas9	negative selection	6713271
169590755	169590778	4	+	27260156	HS294T	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.35976445240858473	[623]	[272,328,349,283]	5	hSpCas9	negative selection	6713272
169590725	169590748	4	-	27260156	HCC44	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.07051330324501515	[725]	[1054,761,952,723]	1	hSpCas9	negative selection	6770263
169599171	169599194	4	-	27260156	HCC44	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.5875667275306808	[50]	[50,8,13,78]	-7	hSpCas9	negative selection	6770264
169589468	169589491	4	-	27260156	HCC44	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.3236818995750008	[141]	[194,162,302,164]	5	hSpCas9	negative selection	6770265
169599122	169599145	4	-	27260156	HCC44	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.4605237428728812	[175]	[109,143,177,147]	-6	hSpCas9	negative selection	6834522
169590755	169590778	4	+	27260156	HCC44	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.24454151891252035	[623]	[1169,699,873,655]	4	hSpCas9	negative selection	6834523
169590725	169590748	4	-	27260156	G402	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.16509226042879122	[725]	[1092,653,1225,990]	2	hSpCas9	negative selection	6891514
169599171	169599194	4	-	27260156	G402	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.5765581955184143	[50]	[251,79,50,13]	7	hSpCas9	negative selection	6891515
169589468	169589491	4	-	27260156	G402	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.1980114732945948	[141]	[288,67,207,232]	2	hSpCas9	negative selection	6891516
169599122	169599145	4	-	27260156	G402	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	0.7807807367398647	[175]	[228,106,903,206]	8	hSpCas9	negative selection	6955773
169590755	169590778	4	+	27260156	G402	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.7267044942266179	[623]	[1248,1275,1471,1047]	8	hSpCas9	negative selection	6955774
169590725	169590748	4	-	27260156	L33	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.015812896822117062	[725]	[814,259,449,767]	0	hSpCas9	negative selection	7012765
169599171	169599194	4	-	27260156	L33	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.6644266924193571	[50]	[65,29,59,88]	9	hSpCas9	negative selection	7012766
169589468	169589491	4	-	27260156	L33	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.16610037741940387	[141]	[173,65,83,167]	3	hSpCas9	negative selection	7012767
169599122	169599145	4	-	27260156	L33	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-1.0358908470084813	[175]	[94,48,46,53]	-9	hSpCas9	negative selection	7077024
169590755	169590778	4	+	27260156	L33	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	-0.13611598832829225	[623]	[372,260,412,608]	-3	hSpCas9	negative selection	7077025
169590725	169590748	4	-	27260156	K562	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.6350776102950013	[725]	[974,984,929,1093]	7	hSpCas9	negative selection	7134016
169599171	169599194	4	-	27260156	K562	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.495442086225641	[50]	[15,0,91,17]	-5	hSpCas9	negative selection	7134017
169589468	169589491	4	-	27260156	K562	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.2607314244962713	[141]	[269,170,30,142]	3	hSpCas9	negative selection	7134018
169599122	169599145	4	-	27260156	K562	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.07707322599620103	[175]	[45,70,330,132]	-1	hSpCas9	negative selection	7198275
169590755	169590778	4	+	27260156	K562	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.7499877855630313	[623]	[880,1332,845,704]	8	hSpCas9	negative selection	7198276
169590725	169590748	4	-	27260156	HT29	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.32434578747943565	[725]	[1165,999,1309,950]	6	hSpCas9	negative selection	7255267
169599171	169599194	4	-	27260156	HT29	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.43816881286888965	[50]	[115,127,52,48]	8	hSpCas9	negative selection	7255268
169589468	169589491	4	-	27260156	HT29	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.4671207651200988	[141]	[254,307,140,253]	8	hSpCas9	negative selection	7255269
169599122	169599145	4	-	27260156	HT29	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.10606267112006429	[175]	[314,214,146,134]	-2	hSpCas9	negative selection	7319526
169590755	169590778	4	+	27260156	HT29	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.33646571459525726	[623]	[1013,1042,894,891]	6	hSpCas9	negative selection	7319527
169590725	169590748	4	-	27260156	MHHES1	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.11990205601292292	[725]	[1015,749,871,456]	-2	hSpCas9	negative selection	7376518
169599171	169599194	4	-	27260156	MHHES1	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.07766558483816643	[50]	[65,59,91,10]	-1	hSpCas9	negative selection	7376519
169589468	169589491	4	-	27260156	MHHES1	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.6079286456323327	[141]	[245,369,199,180]	9	hSpCas9	negative selection	7376520
169599122	169599145	4	-	27260156	MHHES1	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.45940174468720985	[175]	[259,190,116,46]	-7	hSpCas9	negative selection	7440777
169590755	169590778	4	+	27260156	MHHES1	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.4292704733319121	[623]	[1257,1080,911,636]	7	hSpCas9	negative selection	7440778
169590725	169590748	4	-	27260156	MEWO	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.039037025404290104	[725]	[608,664,698]	-1	hSpCas9	negative selection	7497769
169599171	169599194	4	-	27260156	MEWO	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.5798990929232231	[50]	[70,46,95]	9	hSpCas9	negative selection	7497770
169589468	169589491	4	-	27260156	MEWO	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.4095605568855951	[141]	[193,123,214]	8	hSpCas9	negative selection	7497771
169599122	169599145	4	-	27260156	MEWO	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.7972959712609327	[175]	[119,118,49]	-9	hSpCas9	negative selection	7562028
169590755	169590778	4	+	27260156	MEWO	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.23877449907853515	[623]	[811,750,526]	5	hSpCas9	negative selection	7562029
169590725	169590748	4	-	27260156	LNCAPCLONEFGC	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.13868418696370144	[725]	[715,812,603,738]	2	hSpCas9	negative selection	7619020
169599171	169599194	4	-	27260156	LNCAPCLONEFGC	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.44365882887724073	[50]	[40,17,48,30]	-6	hSpCas9	negative selection	7619021
169589468	169589491	4	-	27260156	LNCAPCLONEFGC	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.6506851099882627	[141]	[355,140,210,101]	8	hSpCas9	negative selection	7619022
169599122	169599145	4	-	27260156	LNCAPCLONEFGC	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.40601404957349074	[175]	[65,120,165,135]	-6	hSpCas9	negative selection	7683279
169590755	169590778	4	+	27260156	LNCAPCLONEFGC	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.4134858637293373	[623]	[692,817,787,713]	6	hSpCas9	negative selection	7683280
169590725	169590748	4	-	27260156	PANC0327	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.2279514798699228	[725]	[826,655,904,651]	3	hSpCas9	negative selection	7740271
169599171	169599194	4	-	27260156	PANC0327	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.3745996460429346	[50]	[37,20,35,142]	5	hSpCas9	negative selection	7740272
169589468	169589491	4	-	27260156	PANC0327	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.6276404721477908	[141]	[199,386,80,86]	8	hSpCas9	negative selection	7740273
169599122	169599145	4	-	27260156	PANC0327	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.6729925310470368	[175]	[161,31,112,98]	-7	hSpCas9	negative selection	7804530
169590755	169590778	4	+	27260156	PANC0327	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	-0.065763536956577	[623]	[762,391,445,553]	-1	hSpCas9	negative selection	7804531
169590725	169590748	4	-	27260156	NCIH2009	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.3677834324072148	[725]	[920,817,643]	-5	hSpCas9	negative selection	7861522
169599171	169599194	4	-	27260156	NCIH2009	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.11158306001598728	[50]	[25,62,131]	1	hSpCas9	negative selection	7861523
169589468	169589491	4	-	27260156	NCIH2009	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.6334019578857262	[141]	[340,214,374]	8	hSpCas9	negative selection	7861524
169599122	169599145	4	-	27260156	NCIH2009	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-2.012692621181598	[175]	[40,47,89]	-9	hSpCas9	negative selection	7925781
169590755	169590778	4	+	27260156	NCIH2009	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.2875527992044667	[623]	[1200,1042,973]	4	hSpCas9	negative selection	7925782
169590725	169590748	4	-	27260156	NCIH1373	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.1950544662745895	[725]	[969,345,646,255]	-2	hSpCas9	negative selection	7982773
169599171	169599194	4	-	27260156	NCIH1373	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.3571082362725422	[50]	[70,40,15,45]	3	hSpCas9	negative selection	7982774
169589468	169589491	4	-	27260156	NCIH1373	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	1.3205578538635927	[141]	[239,225,674,114]	9	hSpCas9	negative selection	7982775
169599122	169599145	4	-	27260156	NCIH1373	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.761354404866915	[175]	[163,38,81,52]	-6	hSpCas9	negative selection	8047032
169590755	169590778	4	+	27260156	NCIH1373	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.8079420356134972	[623]	[1580,998,666,464]	7	hSpCas9	negative selection	8047033
169590725	169590748	4	-	27260156	PATU8902	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.12384115288610409	[725]	[1187,813,1061,1374]	1	hSpCas9	negative selection	8104024
169599171	169599194	4	-	27260156	PATU8902	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.19854297871558702	[50]	[51,20,66,203]	2	hSpCas9	negative selection	8104025
169589468	169589491	4	-	27260156	PATU8902	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.9141991162013652	[141]	[553,123,63,883]	8	hSpCas9	negative selection	8104026
169599122	169599145	4	-	27260156	PATU8902	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.06143871154064384	[175]	[103,539,92,240]	0	hSpCas9	negative selection	8168283
169590755	169590778	4	+	27260156	PATU8902	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	1.0410278572197633	[623]	[1551,2182,885,2887]	9	hSpCas9	negative selection	8168284
169590725	169590748	4	-	27260156	PANC1	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.43845706110947225	[725]	[692,909,1005,784]	7	hSpCas9	negative selection	8225275
169599171	169599194	4	-	27260156	PANC1	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.29099684347969923	[50]	[70,67,50,29]	5	hSpCas9	negative selection	8225276
169589468	169589491	4	-	27260156	PANC1	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.3179681994525243	[141]	[135,258,97,136]	5	hSpCas9	negative selection	8225277
169599122	169599145	4	-	27260156	PANC1	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.2539749593772067	[175]	[108,95,204,94]	-4	hSpCas9	negative selection	8289534
169590755	169590778	4	+	27260156	PANC1	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.2507275633153954	[623]	[705,859,536,502]	4	hSpCas9	negative selection	8289535
169590725	169590748	4	-	27260156	PANC0813	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.10753230530463098	[725]	[905,905,863,991]	1	hSpCas9	negative selection	8346526
169599171	169599194	4	-	27260156	PANC0813	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.4786718294959469	[50]	[93,76,101,62]	7	hSpCas9	negative selection	8346527
169589468	169589491	4	-	27260156	PANC0813	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.40783468875063256	[141]	[196,114,362,208]	6	hSpCas9	negative selection	8346528
169599122	169599145	4	-	27260156	PANC0813	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.3042611992114015	[175]	[135,135,92,293]	-5	hSpCas9	negative selection	8410785
169590755	169590778	4	+	27260156	PANC0813	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.23159505704582456	[623]	[751,717,1011,944]	4	hSpCas9	negative selection	8410786
169590725	169590748	4	-	27260156	RDES	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.3387428602341158	[725]	[1013,1010,1090,1091]	5	hSpCas9	negative selection	8467777
169599171	169599194	4	-	27260156	RDES	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.34777450428542256	[50]	[38,46,63,32]	-5	hSpCas9	negative selection	8467778
169589468	169589491	4	-	27260156	RDES	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.43039192784526636	[141]	[191,135,224,355]	7	hSpCas9	negative selection	8467779
169599122	169599145	4	-	27260156	RDES	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.03009083320885808	[175]	[153,180,149,331]	0	hSpCas9	negative selection	8532036
169590755	169590778	4	+	27260156	RDES	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.4905383964216537	[623]	[856,1066,1039,1074]	7	hSpCas9	negative selection	8532037
169590725	169590748	4	-	27260156	PC3	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.17291246612217925	[725]	[655,757,1308,700]	4	hSpCas9	negative selection	8589028
169599171	169599194	4	-	27260156	PC3	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.548306232453303	[50]	[30,44,37,21]	-8	hSpCas9	negative selection	8589029
169589468	169589491	4	-	27260156	PC3	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.25006503592558404	[141]	[122,233,215,112]	5	hSpCas9	negative selection	8589030
169599122	169599145	4	-	27260156	PC3	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.02145809190428838	[175]	[130,145,248,195]	0	hSpCas9	negative selection	8653287
169590755	169590778	4	+	27260156	PC3	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.23610868694099435	[623]	[463,690,1103,869]	5	hSpCas9	negative selection	8653288
169590725	169590748	4	-	27260156	PATU8988T	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.3801181242238538	[725]	[1001,652,964,1014]	6	hSpCas9	negative selection	8710279
169599171	169599194	4	-	27260156	PATU8988T	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-1.212297538554271	[50]	[17,15,23,26]	-9	hSpCas9	negative selection	8710280
169589468	169589491	4	-	27260156	PATU8988T	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.18275962778189392	[141]	[67,254,77,238]	3	hSpCas9	negative selection	8710281
169599122	169599145	4	-	27260156	PATU8988T	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.5287630904995673	[175]	[54,90,240,76]	-7	hSpCas9	negative selection	8774538
169590755	169590778	4	+	27260156	PATU8988T	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.08208668256176072	[623]	[811,402,651,665]	1	hSpCas9	negative selection	8774539
169590725	169590748	4	-	27260156	T47D	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.23027682626351942	[725]	[1001,952,1007,1008]	5	hSpCas9	negative selection	8831530
169599171	169599194	4	-	27260156	T47D	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.033390191348031006	[50]	[48,60,59,61]	0	hSpCas9	negative selection	8831531
169589468	169589491	4	-	27260156	T47D	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.5043369717178054	[141]	[255,157,180,354]	9	hSpCas9	negative selection	8831532
169599122	169599145	4	-	27260156	T47D	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.3443925085100803	[175]	[166,200,118,155]	-6	hSpCas9	negative selection	8895789
169590755	169590778	4	+	27260156	T47D	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.31734168385845524	[623]	[813,955,993,850]	6	hSpCas9	negative selection	8895790
169590725	169590748	4	-	27260156	SU8686	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.044466848236960566	[725]	[659,685,698,756]	-1	hSpCas9	negative selection	8952781
169599171	169599194	4	-	27260156	SU8686	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.5284525008296748	[50]	[39,40,29,25]	-7	hSpCas9	negative selection	8952782
169589468	169589491	4	-	27260156	SU8686	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.9151087408906459	[141]	[320,152,305,293]	9	hSpCas9	negative selection	8952783
169599122	169599145	4	-	27260156	SU8686	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.9127782699905409	[175]	[72,120,101,68]	-8	hSpCas9	negative selection	9017040
169590755	169590778	4	+	27260156	SU8686	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.3178444946540198	[623]	[688,569,765,1150]	5	hSpCas9	negative selection	9017041
169590725	169590748	4	-	27260156	SKES1	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.3697221631361215	[725]	[1035,799,1017,881]	7	hSpCas9	negative selection	9074032
169599171	169599194	4	-	27260156	SKES1	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.07571957110485972	[50]	[63,27,69,31]	-2	hSpCas9	negative selection	9074033
169589468	169589491	4	-	27260156	SKES1	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.08022660133420967	[141]	[219,120,121,141]	1	hSpCas9	negative selection	9074034
169599122	169599145	4	-	27260156	SKES1	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.6142238464422478	[175]	[153,126,109,77]	-8	hSpCas9	negative selection	9138291
169590755	169590778	4	+	27260156	SKES1	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.3551307924213423	[623]	[994,724,775,709]	7	hSpCas9	negative selection	9138292
169590725	169590748	4	-	27260156	TOV112D	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.05485378947599695	[725]	[699,619,753,704]	-1	hSpCas9	negative selection	9195283
169599171	169599194	4	-	27260156	TOV112D	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.5505503211114209	[50]	[4,25,41,60]	-8	hSpCas9	negative selection	9195284
169589468	169589491	4	-	27260156	TOV112D	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.2571672880012339	[141]	[99,183,180,210]	5	hSpCas9	negative selection	9195285
169599122	169599145	4	-	27260156	TOV112D	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.7718923676865903	[175]	[92,129,146,49]	-9	hSpCas9	negative selection	9259542
169590755	169590778	4	+	27260156	TOV112D	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.4516211841815914	[623]	[932,596,811,1013]	8	hSpCas9	negative selection	9259543
169590725	169590748	4	-	27260156	TC71	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	-0.012023906206745982	[725]	[703,820,789,772]	0	hSpCas9	negative selection	9316534
169599171	169599194	4	-	27260156	TC71	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	0.30060983749256787	[50]	[78,85,57,44]	7	hSpCas9	negative selection	9316535
169589468	169589491	4	-	27260156	TC71	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.3137421136013394	[141]	[133,185,217,220]	7	hSpCas9	negative selection	9316536
169599122	169599145	4	-	27260156	TC71	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.41253973975808256	[175]	[180,109,136,137]	-8	hSpCas9	negative selection	9380793
169590755	169590778	4	+	27260156	TC71	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.2269455836519751	[623]	[652,867,765,851]	5	hSpCas9	negative selection	9380794
169590725	169590748	4	-	27260156	TC32	viability	TCATCGAGACATTAAATCTCAGG	NEK1	ENSG00000137601	0.08785941237758965	[725]	[682,637,788,797]	1	hSpCas9	negative selection	9437785
169599171	169599194	4	-	27260156	TC32	viability	TGGCTCTCTCTACATAGTAATGG	NEK1	ENSG00000137601	-0.566000858793628	[50]	[33,1,58,49]	-8	hSpCas9	negative selection	9437786
169589468	169589491	4	-	27260156	TC32	viability	ACAGTACAACTTGGAGATTTTGG	NEK1	ENSG00000137601	0.03940568480592188	[141]	[110,90,234,141]	0	hSpCas9	negative selection	9437787
169599122	169599145	4	-	27260156	TC32	viability	AAGCGAATAAATGCTCAGAAAGG	NEK1	ENSG00000137601	-0.29797993212337115	[175]	[182,105,155,89]	-5	hSpCas9	negative selection	9502044
169590755	169590778	4	+	27260156	TC32	viability	TTCTATCATGTACATGTTTCAGG	NEK1	ENSG00000137601	0.34043167969104143	[623]	[833,401,990,867]	6	hSpCas9	negative selection	9502045
169479473	169479496	4	-	27869803	HPAFII	viability after 27 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.018802088427468666	[616]	[362,299,NaN]	0	hSpCas9	negative selection	9587496
169508797	169508820	4	-	27869803	HPAFII	viability after 27 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.1518540131187014	[111]	[40,62,NaN]	-1	hSpCas9	negative selection	9587497
169537816	169537839	4	-	27869803	HPAFII	viability after 27 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-1.4201683752120215	[204]	[66,17,NaN]	-7	hSpCas9	negative selection	9587498
169555931	169555954	4	-	27869803	HPAFII	viability after 27 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-0.7295565597066068	[407]	[148,112,NaN]	-5	hSpCas9	negative selection	9587499
169576995	169577018	4	+	27869803	HPAFII	viability after 27 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.5453918346708668	[49]	[9,61,NaN]	4	hSpCas9	negative selection	9587500
169599153	169599176	4	-	27869803	HPAFII	viability after 27 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-3.451232704053309	[86]	[7,0,NaN]	-9	hSpCas9	negative selection	9587501
169479473	169479496	4	-	27869803	HPAFII	viability after 15 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.06221221944488131	[616]	[991,505,NaN]	0	hSpCas9	negative selection	9669945
169508797	169508820	4	-	27869803	HPAFII	viability after 15 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.2444544907953773	[111]	[268,63,NaN]	3	hSpCas9	negative selection	9669946
169537816	169537839	4	-	27869803	HPAFII	viability after 15 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-0.35769357505044286	[204]	[240,128,NaN]	-4	hSpCas9	negative selection	9669947
169555931	169555954	4	-	27869803	HPAFII	viability after 15 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-0.1841539142901888	[407]	[491,319,NaN]	-2	hSpCas9	negative selection	9669948
169576995	169577018	4	+	27869803	HPAFII	viability after 15 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-0.30377408077851165	[49]	[45,41,NaN]	-3	hSpCas9	negative selection	9669949
169599153	169599176	4	-	27869803	HPAFII	viability after 15 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-0.28072721602926975	[86]	[92,66,NaN]	-3	hSpCas9	negative selection	9669950
169479473	169479496	4	-	27869803	ASPC1	viability after 36 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.23114472638986394	[1130]	[248,320,721]	2	hSpCas9	negative selection	9752394
169508797	169508820	4	-	27869803	ASPC1	viability after 36 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.043313948569606686	[370]	[138,68,159]	0	hSpCas9	negative selection	9752395
169537816	169537839	4	-	27869803	ASPC1	viability after 36 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-0.3253887725702792	[461]	[200,29,154]	-3	hSpCas9	negative selection	9752396
169555931	169555954	4	-	27869803	ASPC1	viability after 36 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.216799279537093	[1151]	[443,650,332]	2	hSpCas9	negative selection	9752397
169576995	169577018	4	+	27869803	ASPC1	viability after 36 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.44595183027672913	[56]	[19,12,42]	4	hSpCas9	negative selection	9752398
169599153	169599176	4	-	27869803	ASPC1	viability after 36 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	1.0576916462846453	[348]	[397,237,161]	8	hSpCas9	negative selection	9752399
169479473	169479496	4	-	27869803	PATU8988S	viability after 35 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.3164918300242042	[1033]	[560,475,NaN]	3	hSpCas9	negative selection	9834843
169508797	169508820	4	-	27869803	PATU8988S	viability after 35 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-1.0236437202526107	[269]	[47,58,NaN]	-7	hSpCas9	negative selection	9834844
169537816	169537839	4	-	27869803	PATU8988S	viability after 35 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.7101780066676038	[317]	[222,195,NaN]	7	hSpCas9	negative selection	9834845
169555931	169555954	4	-	27869803	PATU8988S	viability after 35 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	0.0012206888370188684	[813]	[378,276,NaN]	0	hSpCas9	negative selection	9834846
169576995	169577018	4	+	27869803	PATU8988S	viability after 35 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.7477312683717994	[78]	[20,85,NaN]	7	hSpCas9	negative selection	9834847
169599153	169599176	4	-	27869803	PATU8988S	viability after 35 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	0.47413239702127274	[174]	[55,139,NaN]	5	hSpCas9	negative selection	9834848
169479473	169479496	4	-	27869803	PATU8988S	viability after 31 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	0.37538923634035093	[1033]	[496,582,NaN]	4	hSpCas9	negative selection	9917292
169508797	169508820	4	-	27869803	PATU8988S	viability after 31 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.9816539499736974	[269]	[50,58,NaN]	-7	hSpCas9	negative selection	9917293
169537816	169537839	4	-	27869803	PATU8988S	viability after 31 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	0.5264747150986986	[317]	[201,157,NaN]	6	hSpCas9	negative selection	9917294
169555931	169555954	4	-	27869803	PATU8988S	viability after 31 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-0.19896226814246898	[813]	[302,256,NaN]	-2	hSpCas9	negative selection	9917295
169576995	169577018	4	+	27869803	PATU8988S	viability after 31 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	-1.3592027797889301	[78]	[6,18,NaN]	-8	hSpCas9	negative selection	9917296
169599153	169599176	4	-	27869803	PATU8988S	viability after 31 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	0.43896902648305414	[174]	[76,116,NaN]	5	hSpCas9	negative selection	9917297
169479473	169479496	4	-	27869803	HPAFII	viability after 35 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	-0.21390206640150522	[616]	[305,227,NaN]	-1	hSpCas9	negative selection	9999741
169508797	169508820	4	-	27869803	HPAFII	viability after 35 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	0.23280024193011872	[111]	[28,108,NaN]	1	hSpCas9	negative selection	9999742
169537816	169537839	4	-	27869803	HPAFII	viability after 35 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-3.162223765446125	[204]	[20,0,NaN]	-8	hSpCas9	negative selection	9999743
169555931	169555954	4	-	27869803	HPAFII	viability after 35 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-1.2219260466885244	[407]	[128,42,NaN]	-6	hSpCas9	negative selection	9999744
169576995	169577018	4	+	27869803	HPAFII	viability after 35 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.5516872324897184	[49]	[6,70,NaN]	3	hSpCas9	negative selection	9999745
169599153	169599176	4	-	27869803	HPAFII	viability after 35 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-5.460854942852054	[86]	[0,0,NaN]	-9	hSpCas9	negative selection	9999746
169479473	169479496	4	-	27869803	HPAFII	viability after 31 days	CCTTTGGGACAGCATGAAACAGG	NEK1	ENSG00000137601	-0.21914818207812403	[616]	[307,285,NaN]	-1	hSpCas9	negative selection	10082190
169508797	169508820	4	-	27869803	HPAFII	viability after 31 days	GGCAGGCCCAGCTCTTCAAGAGG	NEK1	ENSG00000137601	-0.2073128465151446	[111]	[41,67,NaN]	-1	hSpCas9	negative selection	10082191
169537816	169537839	4	-	27869803	HPAFII	viability after 31 days	CAGAATAAAGCTCGAGCCGAAGG	NEK1	ENSG00000137601	-3.3369560027846834	[204]	[19,1,NaN]	-9	hSpCas9	negative selection	10082192
169555931	169555954	4	-	27869803	HPAFII	viability after 31 days	ATGGTCGAGGTCTTCCAGAAAGG	NEK1	ENSG00000137601	-1.318519643620668	[407]	[120,59,NaN]	-7	hSpCas9	negative selection	10082193
169576995	169577018	4	+	27869803	HPAFII	viability after 31 days	GGTATTCCATATTTAGCGGCAGG	NEK1	ENSG00000137601	0.5625665731535641	[49]	[28,54,NaN]	4	hSpCas9	negative selection	10082194
169599153	169599176	4	-	27869803	HPAFII	viability after 31 days	AATGGATTACTGTGAGGGAGGGG	NEK1	ENSG00000137601	-2.9739837223823464	[86]	[10,0,NaN]	-8	hSpCas9	negative selection	10082195
169585473	169585496	4	+	28162770	P31FUJ	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	0.2651035620375542	[147]	[208]	3	hSpCas9	negative selection	10199256
169585441	169585464	4	+	28162770	P31FUJ	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.5376567859427993	[482]	[390]	-4	hSpCas9	negative selection	10199257
169507739	169507762	4	-	28162770	P31FUJ	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	0.07839948387992524	[431]	[535]	0	hSpCas9	negative selection	10199258
169599155	169599178	4	-	28162770	P31FUJ	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	0.034136655875331434	[245]	[295]	0	hSpCas9	negative selection	10199259
169588706	169588729	4	-	28162770	P31FUJ	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.03272436896546593	[120]	[138]	0	hSpCas9	negative selection	10199260
169562149	169562172	4	-	28162770	P31FUJ	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.2967078499590593	[220]	[318]	3	hSpCas9	negative selection	10199261
169561871	169561894	4	-	28162770	P31FUJ	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.15422091869486865	[324]	[424]	1	hSpCas9	negative selection	10199262
169463335	169463358	4	-	28162770	P31FUJ	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-1.5973746367102346	[102]	[39]	-7	hSpCas9	negative selection	10199263
169424634	169424657	4	+	28162770	P31FUJ	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.37233817824440774	[70]	[107]	4	hSpCas9	negative selection	10199264
169406664	169406687	4	+	28162770	P31FUJ	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.17075628642116825	[693]	[917]	1	hSpCas9	negative selection	10199265
169585473	169585496	4	+	28162770	TF1	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	NaN	[NaN]	[124]	1	hSpCas9	negative selection	10368622
169585441	169585464	4	+	28162770	TF1	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	NaN	[NaN]	[421]	1	hSpCas9	negative selection	10368623
169507739	169507762	4	-	28162770	TF1	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	NaN	[NaN]	[326]	1	hSpCas9	negative selection	10368624
169599155	169599178	4	-	28162770	TF1	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	NaN	[NaN]	[314]	1	hSpCas9	negative selection	10368625
169588706	169588729	4	-	28162770	TF1	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	NaN	[NaN]	[92]	1	hSpCas9	negative selection	10368626
169562149	169562172	4	-	28162770	TF1	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	NaN	[NaN]	[135]	1	hSpCas9	negative selection	10368627
169561871	169561894	4	-	28162770	TF1	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	NaN	[NaN]	[171]	1	hSpCas9	negative selection	10368628
169463335	169463358	4	-	28162770	TF1	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	NaN	[NaN]	[59]	1	hSpCas9	negative selection	10368629
169424634	169424657	4	+	28162770	TF1	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	NaN	[NaN]	[55]	1	hSpCas9	negative selection	10368630
169406664	169406687	4	+	28162770	TF1	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	NaN	[NaN]	[585]	1	hSpCas9	negative selection	10368631
169585473	169585496	4	+	28162770	SKM1	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.7654181696556379	[49]	[51]	-5	hSpCas9	negative selection	10537988
169585441	169585464	4	+	28162770	SKM1	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.7318038890504275	[123]	[65]	-8	hSpCas9	negative selection	10537989
169507739	169507762	4	-	28162770	SKM1	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.48758265895144626	[137]	[173]	-4	hSpCas9	negative selection	10537990
169599155	169599178	4	-	28162770	SKM1	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.5548976278791649	[58]	[70]	-4	hSpCas9	negative selection	10537991
169588706	169588729	4	-	28162770	SKM1	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-1.4409115306664995	[42]	[27]	-7	hSpCas9	negative selection	10537992
169562149	169562172	4	-	28162770	SKM1	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.023964673305573392	[78]	[141]	0	hSpCas9	negative selection	10537993
169561871	169561894	4	-	28162770	SKM1	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.2381192944155654	[46]	[97]	2	hSpCas9	negative selection	10537994
169463335	169463358	4	-	28162770	SKM1	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.48758265895144626	[22]	[28]	-4	hSpCas9	negative selection	10537995
169424634	169424657	4	+	28162770	SKM1	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.025995208532944236	[9]	[17]	0	hSpCas9	negative selection	10537996
169406664	169406687	4	+	28162770	SKM1	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.5910472631821339	[143]	[168]	-5	hSpCas9	negative selection	10537997
169585473	169585496	4	+	28162770	SKM1	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.7654181696556379	[49]	[51]	-5	hSpCas9	negative selection	10707354
169585441	169585464	4	+	28162770	SKM1	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.7318038890504275	[123]	[65]	-8	hSpCas9	negative selection	10707355
169507739	169507762	4	-	28162770	SKM1	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.48758265895144626	[137]	[173]	-4	hSpCas9	negative selection	10707356
169599155	169599178	4	-	28162770	SKM1	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.5548976278791649	[58]	[70]	-4	hSpCas9	negative selection	10707357
169588706	169588729	4	-	28162770	SKM1	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-1.4409115306664995	[42]	[27]	-7	hSpCas9	negative selection	10707358
169562149	169562172	4	-	28162770	SKM1	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.023964673305573392	[78]	[141]	0	hSpCas9	negative selection	10707359
169561871	169561894	4	-	28162770	SKM1	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.2381192944155654	[46]	[97]	2	hSpCas9	negative selection	10707360
169463335	169463358	4	-	28162770	SKM1	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.48758265895144626	[22]	[28]	-4	hSpCas9	negative selection	10707361
169424634	169424657	4	+	28162770	SKM1	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.025995208532944236	[9]	[17]	0	hSpCas9	negative selection	10707362
169406664	169406687	4	+	28162770	SKM1	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.5910472631821339	[143]	[168]	-5	hSpCas9	negative selection	10707363
169585473	169585496	4	+	28162770	PL21	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.5774679641742319	[167]	[48]	-4	hSpCas9	negative selection	10876720
169585441	169585464	4	+	28162770	PL21	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.14960642453780443	[287]	[112]	-1	hSpCas9	negative selection	10876721
169507739	169507762	4	-	28162770	PL21	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	0.12885252985402462	[642]	[305]	1	hSpCas9	negative selection	10876722
169599155	169599178	4	-	28162770	PL21	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.2626510162378917	[214]	[77]	-2	hSpCas9	negative selection	10876723
169588706	169588729	4	-	28162770	PL21	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.43307805483905715	[151]	[48]	-3	hSpCas9	negative selection	10876724
169562149	169562172	4	-	28162770	PL21	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	-0.154817280785513	[242]	[94]	-1	hSpCas9	negative selection	10876725
169561871	169561894	4	-	28162770	PL21	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-2.0222528068471277	[167]	[17]	-8	hSpCas9	negative selection	10876726
169463335	169463358	4	-	28162770	PL21	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	0.45597851891890984	[66]	[39]	4	hSpCas9	negative selection	10876727
169424634	169424657	4	+	28162770	PL21	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.6526518191868269	[37]	[25]	5	hSpCas9	negative selection	10876728
169406664	169406687	4	+	28162770	PL21	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.07238624032177143	[487]	[201]	0	hSpCas9	negative selection	10876729
169585473	169585496	4	+	28162770	P31FUJ	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	0.2651035620375542	[147]	[208]	3	hSpCas9	negative selection	11046086
169585441	169585464	4	+	28162770	P31FUJ	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.5376567859427993	[482]	[390]	-4	hSpCas9	negative selection	11046087
169507739	169507762	4	-	28162770	P31FUJ	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	0.07839948387992524	[431]	[535]	0	hSpCas9	negative selection	11046088
169599155	169599178	4	-	28162770	P31FUJ	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	0.034136655875331434	[245]	[295]	0	hSpCas9	negative selection	11046089
169588706	169588729	4	-	28162770	P31FUJ	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.03272436896546593	[120]	[138]	0	hSpCas9	negative selection	11046090
169562149	169562172	4	-	28162770	P31FUJ	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.2967078499590593	[220]	[318]	3	hSpCas9	negative selection	11046091
169561871	169561894	4	-	28162770	P31FUJ	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.15422091869486865	[324]	[424]	1	hSpCas9	negative selection	11046092
169463335	169463358	4	-	28162770	P31FUJ	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-1.5973746367102346	[102]	[39]	-7	hSpCas9	negative selection	11046093
169424634	169424657	4	+	28162770	P31FUJ	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.37233817824440774	[70]	[107]	4	hSpCas9	negative selection	11046094
169406664	169406687	4	+	28162770	P31FUJ	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.17075628642116825	[693]	[917]	1	hSpCas9	negative selection	11046095
169585473	169585496	4	+	28162770	OCIAML5	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.42731391675194147	[110]	[148]	-3	hSpCas9	negative selection	11215452
169585441	169585464	4	+	28162770	OCIAML5	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.14413002530932306	[250]	[409]	-1	hSpCas9	negative selection	11215453
169507739	169507762	4	-	28162770	OCIAML5	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-1.2987496967593062	[337]	[247]	-6	hSpCas9	negative selection	11215454
169599155	169599178	4	-	28162770	OCIAML5	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	1.311599440106633	[76]	[344]	9	hSpCas9	negative selection	11215455
169588706	169588729	4	-	28162770	OCIAML5	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.2881656856706705	[91]	[135]	-2	hSpCas9	negative selection	11215456
169562149	169562172	4	-	28162770	OCIAML5	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	1.6402649519468948	[66]	[376]	9	hSpCas9	negative selection	11215457
169561871	169561894	4	-	28162770	OCIAML5	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	0.39334446707518067	[150]	[357]	3	hSpCas9	negative selection	11215458
169463335	169463358	4	-	28162770	OCIAML5	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.23431413502517162	[57]	[88]	-2	hSpCas9	negative selection	11215459
169424634	169424657	4	+	28162770	OCIAML5	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.3022615755272968	[30]	[68]	2	hSpCas9	negative selection	11215460
169406664	169406687	4	+	28162770	OCIAML5	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.7796496985569008	[252]	[783]	6	hSpCas9	negative selection	11215461
169585473	169585496	4	+	28162770	EOL1	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-1.4315960386547408	[255]	[49]	-7	hSpCas9	negative selection	11384818
169585441	169585464	4	+	28162770	EOL1	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.8791221036792138	[390]	[55]	-8	hSpCas9	negative selection	11384819
169507739	169507762	4	-	28162770	EOL1	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-1.3432245532699225	[914]	[189]	-7	hSpCas9	negative selection	11384820
169599155	169599178	4	-	28162770	EOL1	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.6340030983198259	[432]	[146]	-4	hSpCas9	negative selection	11384821
169588706	169588729	4	-	28162770	EOL1	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-1.1853699205075008	[258]	[59]	-7	hSpCas9	negative selection	11384822
169562149	169562172	4	-	28162770	EOL1	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.15341303742568374	[412]	[241]	1	hSpCas9	negative selection	11384823
169561871	169561894	4	-	28162770	EOL1	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-1.6894437777621536	[250]	[40]	-8	hSpCas9	negative selection	11384824
169463335	169463358	4	-	28162770	EOL1	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	0.057814302433998765	[92]	[50]	0	hSpCas9	negative selection	11384825
169424634	169424657	4	+	28162770	EOL1	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.3395852708493785	[32]	[21]	3	hSpCas9	negative selection	11384826
169406664	169406687	4	+	28162770	EOL1	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.21763223938887322	[617]	[279]	-2	hSpCas9	negative selection	11384827
169585473	169585496	4	+	28162770	MOLM13	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.481484545385453	[279]	[144]	-3	hSpCas9	negative selection	11554184
169585441	169585464	4	+	28162770	MOLM13	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-2.20146530646695	[527]	[82]	-7	hSpCas9	negative selection	11554185
169507739	169507762	4	-	28162770	MOLM13	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.6967875419355818	[685]	[305]	-4	hSpCas9	negative selection	11554186
169599155	169599178	4	-	28162770	MOLM13	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.5786532043924515	[251]	[121]	-3	hSpCas9	negative selection	11554187
169588706	169588729	4	-	28162770	MOLM13	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.35153837281360023	[179]	[101]	-2	hSpCas9	negative selection	11554188
169562149	169562172	4	-	28162770	MOLM13	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.560186228865361	[332]	[354]	4	hSpCas9	negative selection	11554189
169561871	169561894	4	-	28162770	MOLM13	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.8922861826997469	[343]	[133]	-5	hSpCas9	negative selection	11554190
169463335	169463358	4	-	28162770	MOLM13	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.45959811867105316	[96]	[50]	-3	hSpCas9	negative selection	11554191
169424634	169424657	4	+	28162770	MOLM13	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-0.5321106184554214	[93]	[46]	-3	hSpCas9	negative selection	11554192
169406664	169406687	4	+	28162770	MOLM13	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.10708903060395536	[647]	[434]	0	hSpCas9	negative selection	11554193
169585473	169585496	4	+	28162770	HEL	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	0.002634777086739726	[239]	[153]	0	hSpCas9	negative selection	11723550
169585441	169585464	4	+	28162770	HEL	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.1522588835372645	[464]	[133]	-6	hSpCas9	negative selection	11723551
169507739	169507762	4	-	28162770	HEL	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.409316989066163	[648]	[312]	-3	hSpCas9	negative selection	11723552
169599155	169599178	4	-	28162770	HEL	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.32483969030726545	[263]	[134]	-2	hSpCas9	negative selection	11723553
169588706	169588729	4	-	28162770	HEL	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.5084252413332939	[190]	[85]	-3	hSpCas9	negative selection	11723554
169562149	169562172	4	-	28162770	HEL	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.9902431735937356	[245]	[312]	8	hSpCas9	negative selection	11723555
169561871	169561894	4	-	28162770	HEL	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.29770432331918817	[260]	[135]	-2	hSpCas9	negative selection	11723556
169463335	169463358	4	-	28162770	HEL	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-1.7835259227017408	[85]	[15]	-7	hSpCas9	negative selection	11723557
169424634	169424657	4	+	28162770	HEL	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-1.5850670855224556	[88]	[18]	-7	hSpCas9	negative selection	11723558
169406664	169406687	4	+	28162770	HEL	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.5199473432118232	[496]	[221]	-4	hSpCas9	negative selection	11723559
169585473	169585496	4	+	28162770	MV411	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.8261435815983322	[180]	[85]	-5	hSpCas9	negative selection	11892916
169585441	169585464	4	+	28162770	MV411	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.6339179527186056	[279]	[75]	-7	hSpCas9	negative selection	11892917
169507739	169507762	4	-	28162770	MV411	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.7426469803521422	[434]	[218]	-5	hSpCas9	negative selection	11892918
169599155	169599178	4	-	28162770	MV411	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.5115543497134298	[241]	[142]	-4	hSpCas9	negative selection	11892919
169588706	169588729	4	-	28162770	MV411	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.29065834490040676	[105]	[72]	-2	hSpCas9	negative selection	11892920
169562149	169562172	4	-	28162770	MV411	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.282770623895056	[241]	[247]	2	hSpCas9	negative selection	11892921
169561871	169561894	4	-	28162770	MV411	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-1.9595248299840666	[216]	[46]	-8	hSpCas9	negative selection	11892922
169463335	169463358	4	-	28162770	MV411	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.525151953114152	[81]	[47]	-4	hSpCas9	negative selection	11892923
169424634	169424657	4	+	28162770	MV411	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-1.4530021673583167	[51]	[15]	-7	hSpCas9	negative selection	11892924
169406664	169406687	4	+	28162770	MV411	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.8053688083918783	[472]	[227]	-5	hSpCas9	negative selection	11892925
169585473	169585496	4	+	28162770	MONOMAC1	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.4836580732109633	[110]	[90]	-4	hSpCas9	negative selection	12062282
169585441	169585464	4	+	28162770	MONOMAC1	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.747729445695964	[207]	[141]	-6	hSpCas9	negative selection	12062283
169507739	169507762	4	-	28162770	MONOMAC1	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	0.41793822272804193	[332]	[509]	4	hSpCas9	negative selection	12062284
169599155	169599178	4	-	28162770	MONOMAC1	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	0.07902423907047978	[146]	[177]	0	hSpCas9	negative selection	12062285
169588706	169588729	4	-	28162770	MONOMAC1	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.6855365082794185	[93]	[66]	-5	hSpCas9	negative selection	12062286
169562149	169562172	4	-	28162770	MONOMAC1	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.32026870355834036	[210]	[301]	3	hSpCas9	negative selection	12062287
169561871	169561894	4	-	28162770	MONOMAC1	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.6151802572062193	[154]	[115]	-5	hSpCas9	negative selection	12062288
169463335	169463358	4	-	28162770	MONOMAC1	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.5781270144150596	[55]	[42]	-5	hSpCas9	negative selection	12062289
169424634	169424657	4	+	28162770	MONOMAC1	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-0.897476565200646	[25]	[15]	-6	hSpCas9	negative selection	12062290
169406664	169406687	4	+	28162770	MONOMAC1	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.21800065221929033	[338]	[451]	2	hSpCas9	negative selection	12062291
169585473	169585496	4	+	28162770	OCIAML2	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-1.1380544861552904	[26]	[67]	-6	hSpCas9	negative selection	12231648
169585441	169585464	4	+	28162770	OCIAML2	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-1.760136442437146	[65]	[107]	-8	hSpCas9	negative selection	12231649
169507739	169507762	4	-	28162770	OCIAML2	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	-0.40789406989419874	[89]	[375]	-3	hSpCas9	negative selection	12231650
169599155	169599178	4	-	28162770	OCIAML2	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	-0.49977617090167775	[49]	[195]	-4	hSpCas9	negative selection	12231651
169588706	169588729	4	-	28162770	OCIAML2	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.9512556661485819	[29]	[85]	-6	hSpCas9	negative selection	12231652
169562149	169562172	4	-	28162770	OCIAML2	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	0.024629872530763097	[46]	[264]	0	hSpCas9	negative selection	12231653
169561871	169561894	4	-	28162770	OCIAML2	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.3267940526685096	[30]	[136]	-3	hSpCas9	negative selection	12231654
169463335	169463358	4	-	28162770	OCIAML2	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.6055594053282698	[13]	[50]	-4	hSpCas9	negative selection	12231655
169424634	169424657	4	+	28162770	OCIAML2	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	0.766409372058688	[6]	[65]	7	hSpCas9	negative selection	12231656
169406664	169406687	4	+	28162770	OCIAML2	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	-0.5025390731969052	[79]	[312]	-4	hSpCas9	negative selection	12231657
169585473	169585496	4	+	28162770	OCIAML3	viability	GAATAATGCAAAGACACAGGTGG	NEK1	ENSG00000137601	-0.31389972113142783	[48]	[118]	-2	hSpCas9	negative selection	12401014
169585441	169585464	4	+	28162770	OCIAML3	viability	ATAACTGAGACACCAAACTGCGG	NEK1	ENSG00000137601	-0.6089579817901347	[96]	[191]	-5	hSpCas9	negative selection	12401015
169507739	169507762	4	-	28162770	OCIAML3	viability	GAAGTGAAGAGGCTGACATGAGG	NEK1	ENSG00000137601	0.1336950325130748	[124]	[413]	1	hSpCas9	negative selection	12401016
169599155	169599178	4	-	28162770	OCIAML3	viability	GTAATGGATTACTGTGAGGGAGG	NEK1	ENSG00000137601	0.22778879412192687	[70]	[250]	2	hSpCas9	negative selection	12401017
169588706	169588729	4	-	28162770	OCIAML3	viability	GAGCTGGCTCGAACTTGCATAGG	NEK1	ENSG00000137601	-0.05211386149489283	[33]	[98]	0	hSpCas9	negative selection	12401018
169562149	169562172	4	-	28162770	OCIAML3	viability	TGAATACTGGAGAAGAAAGGAGG	NEK1	ENSG00000137601	-0.014921705939676344	[81]	[244]	0	hSpCas9	negative selection	12401019
169561871	169561894	4	-	28162770	OCIAML3	viability	AGGAAGCAGCAAGAAAGAGAAGG	NEK1	ENSG00000137601	-0.09758181420466427	[55]	[157]	0	hSpCas9	negative selection	12401020
169463335	169463358	4	-	28162770	OCIAML3	viability	GGATCTCCAAGAAGAGCCTGGGG	NEK1	ENSG00000137601	-0.3529995408203681	[10]	[25]	-3	hSpCas9	negative selection	12401021
169424634	169424657	4	+	28162770	OCIAML3	viability	CGAGTGAGATCGAAATGCAAAGG	NEK1	ENSG00000137601	-1.5940076403241632	[19]	[19]	-8	hSpCas9	negative selection	12401022
169406664	169406687	4	+	28162770	OCIAML3	viability	TTGACGAACATCTCCTACGGTGG	NEK1	ENSG00000137601	0.6367160556042388	[110]	[520]	6	hSpCas9	negative selection	12401023