Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:5058
• Symbol:PAK1
• Description:p21 (RAC1) activated kinase 1

DataSource: http://genomecrispr.dkfz.de/#!/results/PAK1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
77392411	77392434	11	-	26472758	Jiyoye	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-2.3919119245681304	[173]	[24]	-8	hSpCas9	negative selection	115510
77392440	77392463	11	+	26472758	Jiyoye	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-1.329790212660333	[129]	[38]	-7	hSpCas9	negative selection	115511
77379297	77379320	11	+	26472758	Jiyoye	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	1.053167315624589	[107]	[168]	7	hSpCas9	negative selection	115512
77353535	77353558	11	-	26472758	Jiyoye	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.11171949797970226	[296]	[241]	0	hSpCas9	negative selection	115513
77392458	77392481	11	+	26472758	Jiyoye	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.9076979558475387	[203]	[81]	-6	hSpCas9	negative selection	115514
77355781	77355804	11	+	26472758	Jiyoye	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.19258133072983297	[245]	[211]	1	hSpCas9	negative selection	115515
77349238	77349261	11	-	26472758	Jiyoye	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	-0.1391842799915357	[294]	[201]	-1	hSpCas9	negative selection	115516
77336226	77336249	11	-	26472758	Jiyoye	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.2551292077484478	[181]	[114]	-2	hSpCas9	negative selection	115517
77332777	77332800	11	+	26472758	Jiyoye	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.6993561329991838	[97]	[119]	5	hSpCas9	negative selection	115518
77332752	77332775	11	-	26472758	Jiyoye	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-2.342846365485779	[73]	[10]	-8	hSpCas9	negative selection	115519
77392411	77392434	11	-	26472758	KBM7	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.23918753579393764	[576,318]	[200,149]	-2	hSpCas9	negative selection	306628
77392440	77392463	11	+	26472758	KBM7	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-0.23658936621883675	[223,177]	[85,73]	-2	hSpCas9	negative selection	306629
77379297	77379320	11	+	26472758	KBM7	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.2413968923911739	[566,342]	[289,209]	2	hSpCas9	negative selection	306630
77353535	77353558	11	-	26472758	KBM7	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.8740724260238959	[799,671]	[910,381]	8	hSpCas9	negative selection	306631
77392458	77392481	11	+	26472758	KBM7	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.06193034439257223	[622,304]	[298,150]	0	hSpCas9	negative selection	306632
77355781	77355804	11	+	26472758	KBM7	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.23890703835423355	[1090,1049]	[553,632]	2	hSpCas9	negative selection	306633
77349238	77349261	11	-	26472758	KBM7	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.5087756201731692	[643,740]	[565,381]	5	hSpCas9	negative selection	306634
77336226	77336249	11	-	26472758	KBM7	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.4665719711633523	[346,377]	[86,157]	-4	hSpCas9	negative selection	306635
77332777	77332800	11	+	26472758	KBM7	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.7299566755032442	[242,315]	[48,108]	-6	hSpCas9	negative selection	306636
77332752	77332775	11	-	26472758	KBM7	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-0.46989932334183626	[176,160]	[21,88]	-4	hSpCas9	negative selection	306637
77392411	77392434	11	-	26472758	Raji	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.1255308820838591	[157]	[39]	1	hSpCas9	negative selection	497746
77392440	77392463	11	+	26472758	Raji	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.17449773123213652	[83]	[21]	1	hSpCas9	negative selection	497747
77379297	77379320	11	+	26472758	Raji	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.072618117212923	[335]	[81]	0	hSpCas9	negative selection	497748
77353535	77353558	11	-	26472758	Raji	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.2817432602882458	[396]	[111]	2	hSpCas9	negative selection	497749
77392458	77392481	11	+	26472758	Raji	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.8714286635960902	[284]	[120]	7	hSpCas9	negative selection	497750
77355781	77355804	11	+	26472758	Raji	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.408892089022029	[369]	[113]	4	hSpCas9	negative selection	497751
77349238	77349261	11	-	26472758	Raji	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.6096056050868194	[304]	[107]	6	hSpCas9	negative selection	497752
77336226	77336249	11	-	26472758	Raji	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.07583828868217073	[217]	[47]	0	hSpCas9	negative selection	497753
77332777	77332800	11	+	26472758	Raji	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.9007903960721004	[176]	[21]	-6	hSpCas9	negative selection	497754
77332752	77332775	11	-	26472758	Raji	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.990648547586927	[136]	[7]	-8	hSpCas9	negative selection	497755
77392411	77392434	11	-	26472758	K562	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.24368223380212028	[215]	[153]	-2	hSpCas9	negative selection	688864
77392440	77392463	11	+	26472758	K562	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.3848999512162773	[175]	[193]	3	hSpCas9	negative selection	688865
77379297	77379320	11	+	26472758	K562	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.41192512830611594	[397]	[446]	3	hSpCas9	negative selection	688866
77353535	77353558	11	-	26472758	K562	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.1445468007540548	[612]	[571]	1	hSpCas9	negative selection	688867
77392458	77392481	11	+	26472758	K562	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.7139335749750617	[477]	[245]	-4	hSpCas9	negative selection	688868
77355781	77355804	11	+	26472758	K562	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.1591034807567635	[922]	[869]	1	hSpCas9	negative selection	688869
77349238	77349261	11	-	26472758	K562	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	-0.1668011058734472	[628]	[472]	-1	hSpCas9	negative selection	688870
77336226	77336249	11	-	26472758	K562	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.7385077589746115	[335]	[169]	-4	hSpCas9	negative selection	688871
77332777	77332800	11	+	26472758	K562	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.7744147691696746	[213]	[308]	6	hSpCas9	negative selection	688872
77332752	77332775	11	-	26472758	K562	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-2.8335837843348264	[227]	[26]	-8	hSpCas9	negative selection	688873
77336073	77336096	11	+	26627737	DLD1	viability	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.8091665138938292	[182]	[180,50,70]	7	hSpCas9	negative selection	781182
77337387	77337410	11	-	26627737	DLD1	viability	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.7356682340547926	[282]	[90,173,173]	7	hSpCas9	negative selection	781183
77349246	77349269	11	-	26627737	DLD1	viability	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.6460687093827875	[900]	[203,91,231]	-6	hSpCas9	negative selection	781184
77353536	77353559	11	-	26627737	DLD1	viability	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	-0.06368455723335753	[932]	[353,283,209]	0	hSpCas9	negative selection	781185
77355788	77355811	11	-	26627737	DLD1	viability	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.12437696110199936	[1518]	[476,538,534]	1	hSpCas9	negative selection	781186
77392457	77392480	11	+	26627737	DLD1	viability	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.3255277348523389	[370]	[102,103,73]	-3	hSpCas9	negative selection	781187
77336073	77336096	11	+	26627737	GBM cells	viability after 5 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-0.2890190757073896	[130]	[58,55]	-4	hSpCas9	negative selection	863497
77337387	77337410	11	-	26627737	GBM cells	viability after 5 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.18950400324004074	[241]	[188,106]	3	hSpCas9	negative selection	863498
77349246	77349269	11	-	26627737	GBM cells	viability after 5 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.1128632928906679	[245]	[109,133]	-2	hSpCas9	negative selection	863499
77353536	77353559	11	-	26627737	GBM cells	viability after 5 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.1798415071918874	[413]	[286,215]	3	hSpCas9	negative selection	863500
77355788	77355811	11	-	26627737	GBM cells	viability after 5 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.47677170428928073	[662]	[221,288]	-6	hSpCas9	negative selection	863501
77392457	77392480	11	+	26627737	GBM cells	viability after 5 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.1632909472108895	[178]	[103,110]	2	hSpCas9	negative selection	863502
77336073	77336096	11	+	26627737	GBM cells	viability after 13 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-0.4244005302577696	[130]	[47,56]	-5	hSpCas9	negative selection	945812
77337387	77337410	11	-	26627737	GBM cells	viability after 13 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.2147048406498716	[241]	[132,96]	-3	hSpCas9	negative selection	945813
77349246	77349269	11	-	26627737	GBM cells	viability after 13 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.13550356627442633	[245]	[139,151]	1	hSpCas9	negative selection	945814
77353536	77353559	11	-	26627737	GBM cells	viability after 13 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.48866584777764754	[413]	[339,294]	6	hSpCas9	negative selection	945815
77355788	77355811	11	-	26627737	GBM cells	viability after 13 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.2604942069121068	[662]	[280,315]	-3	hSpCas9	negative selection	945816
77392457	77392480	11	+	26627737	GBM cells	viability after 13 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.17278374508212807	[178]	[98,117]	2	hSpCas9	negative selection	945817
77336073	77336096	11	+	26627737	GBM cells	viability after 21 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.10440766731844908	[130]	[87,65]	1	hSpCas9	negative selection	1028127
77337387	77337410	11	-	26627737	GBM cells	viability after 21 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.013428841395607446	[241]	[135,124]	0	hSpCas9	negative selection	1028128
77349246	77349269	11	-	26627737	GBM cells	viability after 21 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.26474740725487733	[245]	[158,161]	2	hSpCas9	negative selection	1028129
77353536	77353559	11	-	26627737	GBM cells	viability after 21 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.3199741972726444	[413]	[297,264]	3	hSpCas9	negative selection	1028130
77355788	77355811	11	-	26627737	GBM cells	viability after 21 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.30880342612476874	[662]	[283,296]	-3	hSpCas9	negative selection	1028131
77392457	77392480	11	+	26627737	GBM cells	viability after 21 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	1.2156031374312044	[178]	[252,200]	9	hSpCas9	negative selection	1028132
77336073	77336096	11	+	26627737	RPE1	viability after 9 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.32903381459205905	[153]	[60,59]	2	hSpCas9	negative selection	1110442
77337387	77337410	11	-	26627737	RPE1	viability after 9 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.43219442311631573	[1242]	[211,386]	-3	hSpCas9	negative selection	1110443
77349246	77349269	11	-	26627737	RPE1	viability after 9 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.7117096903098451	[1514]	[310,261]	-5	hSpCas9	negative selection	1110444
77353536	77353559	11	-	26627737	RPE1	viability after 9 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	1.1569160157017464	[735]	[439,608]	7	hSpCas9	negative selection	1110445
77355788	77355811	11	-	26627737	RPE1	viability after 9 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.3768394374673145	[1435]	[402,816]	2	hSpCas9	negative selection	1110446
77392457	77392480	11	+	26627737	RPE1	viability after 9 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	1.1859683964201764	[443]	[224,433]	7	hSpCas9	negative selection	1110447
77336073	77336096	11	+	26627737	RPE1	viability after 12 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.8987855459714635	[153]	[47,107]	5	hSpCas9	negative selection	1192757
77337387	77337410	11	-	26627737	RPE1	viability after 12 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.9855495598767471	[1242]	[156,180]	-6	hSpCas9	negative selection	1192758
77349246	77349269	11	-	26627737	RPE1	viability after 12 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-1.1960387822910779	[1514]	[267,81]	-7	hSpCas9	negative selection	1192759
77353536	77353559	11	-	26627737	RPE1	viability after 12 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	1.1321999038055544	[735]	[583,273]	6	hSpCas9	negative selection	1192760
77355788	77355811	11	-	26627737	RPE1	viability after 12 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.8015085934817363	[1435]	[601,746]	5	hSpCas9	negative selection	1192761
77392457	77392480	11	+	26627737	RPE1	viability after 12 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.8270861502420033	[443]	[232,188]	5	hSpCas9	negative selection	1192762
77336073	77336096	11	+	26627737	RPE1	viability after 15 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-0.10108326560564551	[153]	[5,73]	0	hSpCas9	negative selection	1275072
77337387	77337410	11	-	26627737	RPE1	viability after 15 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.44428324096079	[1242]	[290,226]	-3	hSpCas9	negative selection	1275073
77349246	77349269	11	-	26627737	RPE1	viability after 15 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.6230977314805561	[1514]	[488,74]	-4	hSpCas9	negative selection	1275074
77353536	77353559	11	-	26627737	RPE1	viability after 15 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.9634585179815955	[735]	[406,404]	6	hSpCas9	negative selection	1275075
77355788	77355811	11	-	26627737	RPE1	viability after 15 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.8257030015538243	[1435]	[805,636]	5	hSpCas9	negative selection	1275076
77392457	77392480	11	+	26627737	RPE1	viability after 15 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.9545478672447214	[443]	[304,183]	6	hSpCas9	negative selection	1275077
77336073	77336096	11	+	26627737	RPE1	viability after 18 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.5552994101200872	[153]	[33,89]	3	hSpCas9	negative selection	1357387
77337387	77337410	11	-	26627737	RPE1	viability after 18 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-1.1191211351551675	[1242]	[166,140]	-6	hSpCas9	negative selection	1357388
77349246	77349269	11	-	26627737	RPE1	viability after 18 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-1.7013047154710055	[1514]	[175,71]	-7	hSpCas9	negative selection	1357389
77353536	77353559	11	-	26627737	RPE1	viability after 18 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.8883112866399882	[735]	[444,284]	5	hSpCas9	negative selection	1357390
77355788	77355811	11	-	26627737	RPE1	viability after 18 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.6012447266959033	[1435]	[511,668]	4	hSpCas9	negative selection	1357391
77392457	77392480	11	+	26627737	RPE1	viability after 18 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	1.1536417351351553	[443]	[224,310]	6	hSpCas9	negative selection	1357392
77336073	77336096	11	+	26627737	HeLa	viability after 8 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.8116976860338085	[179]	[204,109,113]	6	hSpCas9	negative selection	1439702
77337387	77337410	11	-	26627737	HeLa	viability after 8 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.4144189404988399	[430]	[168,201,62]	-4	hSpCas9	negative selection	1439703
77349246	77349269	11	-	26627737	HeLa	viability after 8 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.0659184908583436	[410]	[188,192,136]	0	hSpCas9	negative selection	1439704
77353536	77353559	11	-	26627737	HeLa	viability after 8 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.41585496779974185	[660]	[539,401,252]	4	hSpCas9	negative selection	1439705
77355788	77355811	11	-	26627737	HeLa	viability after 8 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.16560758190629143	[1176]	[694,206,515]	-1	hSpCas9	negative selection	1439706
77392457	77392480	11	+	26627737	HeLa	viability after 8 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.03033612947173986	[470]	[135,417,67]	0	hSpCas9	negative selection	1439707
77336073	77336096	11	+	26627737	HeLa	viability after 12 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-0.13930705866761417	[179]	[73,27,104]	-1	hSpCas9	negative selection	1522017
77337387	77337410	11	-	26627737	HeLa	viability after 12 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.8391673592950026	[430]	[75,108,111]	-6	hSpCas9	negative selection	1522018
77349246	77349269	11	-	26627737	HeLa	viability after 12 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.500233424894147	[410]	[75,166,110]	-4	hSpCas9	negative selection	1522019
77353536	77353559	11	-	26627737	HeLa	viability after 12 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.006840202242079596	[660]	[191,349,270]	0	hSpCas9	negative selection	1522020
77355788	77355811	11	-	26627737	HeLa	viability after 12 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.713707616460668	[1176]	[234,286,366]	-6	hSpCas9	negative selection	1522021
77392457	77392480	11	+	26627737	HeLa	viability after 12 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.40477745999883263	[470]	[92,132,214]	-4	hSpCas9	negative selection	1522022
77336073	77336096	11	+	26627737	HeLa	viability after 15 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.32122476727592386	[179]	[128,91,49]	2	hSpCas9	negative selection	1604332
77337387	77337410	11	-	26627737	HeLa	viability after 15 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.41855590351900235	[430]	[102,125,167]	-4	hSpCas9	negative selection	1604333
77349246	77349269	11	-	26627737	HeLa	viability after 15 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.292669637659837	[410]	[91,149,170]	-3	hSpCas9	negative selection	1604334
77353536	77353559	11	-	26627737	HeLa	viability after 15 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.09625767322497525	[660]	[304,230,329]	0	hSpCas9	negative selection	1604335
77355788	77355811	11	-	26627737	HeLa	viability after 15 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.8112815804494907	[1176]	[261,251,307]	-6	hSpCas9	negative selection	1604336
77392457	77392480	11	+	26627737	HeLa	viability after 15 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.39913585417178565	[470]	[202,305,249]	3	hSpCas9	negative selection	1604337
77336073	77336096	11	+	26627737	HeLa	viability after 18 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.39468307384725443	[179]	[95,75,49]	3	hSpCas9	negative selection	1686647
77337387	77337410	11	-	26627737	HeLa	viability after 18 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.38160102676683844	[430]	[71,108,167]	-3	hSpCas9	negative selection	1686648
77349246	77349269	11	-	26627737	HeLa	viability after 18 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.32169416624002856	[410]	[58,120,170]	-3	hSpCas9	negative selection	1686649
77353536	77353559	11	-	26627737	HeLa	viability after 18 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.06375734878406059	[660]	[193,188,329]	0	hSpCas9	negative selection	1686650
77355788	77355811	11	-	26627737	HeLa	viability after 18 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.7793781027721537	[1176]	[180,216,307]	-6	hSpCas9	negative selection	1686651
77392457	77392480	11	+	26627737	HeLa	viability after 18 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.4180563253647457	[470]	[142,252,249]	3	hSpCas9	negative selection	1686652
77336073	77336096	11	+	26627737	HCT116	viability after 6 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-1.5548393685495818	[540]	[75,2]	-7	hSpCas9	negative selection	1768962
77337387	77337410	11	-	26627737	HCT116	viability after 6 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.5511637539183469	[441]	[49,45]	-3	hSpCas9	negative selection	1768963
77349246	77349269	11	-	26627737	HCT116	viability after 6 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	2.018750091166325	[472]	[475,204]	8	hSpCas9	negative selection	1768964
77353536	77353559	11	-	26627737	HCT116	viability after 6 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	-0.1580179635352223	[875]	[232,62]	-1	hSpCas9	negative selection	1768965
77355788	77355811	11	-	26627737	HCT116	viability after 6 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.050936965225225395	[1311]	[339,143]	0	hSpCas9	negative selection	1768966
77392457	77392480	11	+	26627737	HCT116	viability after 6 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.3628897386794018	[437]	[123,68]	2	hSpCas9	negative selection	1768967
77336073	77336096	11	+	26627737	HCT116	viability after 8 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	1.00413232562457	[111]	[129,161,79]	8	hSpCas9	negative selection	1851277
77337387	77337410	11	-	26627737	HCT116	viability after 8 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.462661585618924	[345]	[125,164,123]	-5	hSpCas9	negative selection	1851278
77349246	77349269	11	-	26627737	HCT116	viability after 8 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.14703159823732653	[286]	[140,108,177]	-2	hSpCas9	negative selection	1851279
77353536	77353559	11	-	26627737	HCT116	viability after 8 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.3228832926274948	[354]	[241,247,243]	3	hSpCas9	negative selection	1851280
77355788	77355811	11	-	26627737	HCT116	viability after 8 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.4321472598401652	[939]	[462,215,472]	-5	hSpCas9	negative selection	1851281
77392457	77392480	11	+	26627737	HCT116	viability after 8 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.23681757344540844	[342]	[174,192,113]	-3	hSpCas9	negative selection	1851282
77336073	77336096	11	+	26627737	HCT116	viability after 9 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	-0.5201492343734466	[540]	[88,109]	-4	hSpCas9	negative selection	1933592
77337387	77337410	11	-	26627737	HCT116	viability after 9 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.09483376614054717	[441]	[138,87]	0	hSpCas9	negative selection	1933593
77349246	77349269	11	-	26627737	HCT116	viability after 9 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.46089176356405226	[472]	[327,51]	3	hSpCas9	negative selection	1933594
77353536	77353559	11	-	26627737	HCT116	viability after 9 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.38077858844244483	[875]	[241,353]	3	hSpCas9	negative selection	1933595
77355788	77355811	11	-	26627737	HCT116	viability after 9 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.3205324507578602	[1311]	[408,459]	2	hSpCas9	negative selection	1933596
77392457	77392480	11	+	26627737	HCT116	viability after 9 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	1.3531607908269336	[437]	[276,315]	8	hSpCas9	negative selection	1933597
77336073	77336096	11	+	26627737	HCT116	viability after 12 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.635304731567255	[111]	[72,95,106]	6	hSpCas9	negative selection	2015907
77337387	77337410	11	-	26627737	HCT116	viability after 12 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.027292469065597513	[345]	[158,137,238]	0	hSpCas9	negative selection	2015908
77349246	77349269	11	-	26627737	HCT116	viability after 12 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.13523001630391446	[286]	[175,153,78]	-1	hSpCas9	negative selection	2015909
77353536	77353559	11	-	26627737	HCT116	viability after 12 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.10113553519958382	[354]	[149,169,283]	1	hSpCas9	negative selection	2015910
77355788	77355811	11	-	26627737	HCT116	viability after 12 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.06803328022538668	[939]	[412,527,621]	0	hSpCas9	negative selection	2015911
77392457	77392480	11	+	26627737	HCT116	viability after 12 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.1602729406341185	[342]	[121,230,258]	1	hSpCas9	negative selection	2015912
77336073	77336096	11	+	26627737	HCT116	viability after 15 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.7830896876347356	[111]	[119,86,125]	6	hSpCas9	negative selection	2098222
77337387	77337410	11	-	26627737	HCT116	viability after 15 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.07607435849833277	[345]	[253,174,144]	-1	hSpCas9	negative selection	2098223
77349246	77349269	11	-	26627737	HCT116	viability after 15 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.05134056808536637	[286]	[313,102,79]	0	hSpCas9	negative selection	2098224
77353536	77353559	11	-	26627737	HCT116	viability after 15 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.5260633469260725	[354]	[343,342,200]	5	hSpCas9	negative selection	2098225
77355788	77355811	11	-	26627737	HCT116	viability after 15 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.2557041979707675	[939]	[683,440,261]	-3	hSpCas9	negative selection	2098226
77392457	77392480	11	+	26627737	HCT116	viability after 15 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.9517795916009295	[342]	[95,122,85]	-7	hSpCas9	negative selection	2098227
77336073	77336096	11	+	26627737	HCT116	viability after 18 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.7722838324860927	[111]	[136,77,61]	6	hSpCas9	negative selection	2180537
77337387	77337410	11	-	26627737	HCT116	viability after 18 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.18499340789872434	[345]	[223,128,222]	1	hSpCas9	negative selection	2180538
77349246	77349269	11	-	26627737	HCT116	viability after 18 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.5522270622512774	[286]	[366,70,179]	4	hSpCas9	negative selection	2180539
77353536	77353559	11	-	26627737	HCT116	viability after 18 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.807681786183917	[354]	[338,337,217]	6	hSpCas9	negative selection	2180540
77355788	77355811	11	-	26627737	HCT116	viability after 18 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.30955302720443423	[939]	[724,424,547]	2	hSpCas9	negative selection	2180541
77392457	77392480	11	+	26627737	HCT116	viability after 18 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.13897795529679405	[342]	[188,132,129]	-1	hSpCas9	negative selection	2180542
77392375	77392398	11	+	24336569	HL60	viability	TTCTTCTCCTCTGGGTTTGGAGG	PAK1	ENSG00000149269	-1.234905574094281	[445]	[400]	-8	hSpCas9	negative selection	2282482
77392381	77392404	11	+	24336569	HL60	viability	TCCTCTGGGTTTGGAGGCAGAGG	PAK1	ENSG00000149269	0.6538268618450558	[861]	[2869]	5	hSpCas9	negative selection	2282483
77392394	77392417	11	+	24336569	HL60	viability	GAGGCAGAGGTTTAGAACCATGG	PAK1	ENSG00000149269	-0.04340349945482788	[504]	[1036]	0	hSpCas9	negative selection	2282484
77392400	77392423	11	+	24336569	HL60	viability	GAGGTTTAGAACCATGGTTTAGG	PAK1	ENSG00000149269	0.030469178054240098	[532]	[1151]	0	hSpCas9	negative selection	2282485
77392411	77392434	11	-	24336569	HL60	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.6524927436649716	[431]	[1436]	5	hSpCas9	negative selection	2282486
77392426	77392449	11	-	24336569	HL60	viability	GGAGCCGGCAGCAAAGATGCTGG	PAK1	ENSG00000149269	0.47775619978038686	[621]	[1832]	4	hSpCas9	negative selection	2282487
77392440	77392463	11	+	24336569	HL60	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	1.0700547857161598	[297]	[1323]	7	hSpCas9	negative selection	2282488
77392447	77392470	11	-	24336569	HL60	viability	AGAAATACCAGCACTATGATTGG	PAK1	ENSG00000149269	-0.4107717258818564	[146]	[233]	-4	hSpCas9	negative selection	2282489
77392453	77392476	11	+	24336569	HL60	viability	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	0.3461004965261105	[128]	[346]	3	hSpCas9	negative selection	2282490
77392507	77392530	11	-	24336569	HL60	viability	GTGGTGACAATGTCAAATAACGG	PAK1	ENSG00000149269	2.3300854049584565	[219]	[2340]	9	hSpCas9	negative selection	2282491
77392375	77392398	11	+	24336569	KBM7	viability	TTCTTCTCCTCTGGGTTTGGAGG	PAK1	ENSG00000149269	0.06217695998309991	[314]	[896]	0	hSpCas9	negative selection	2349659
77392381	77392404	11	+	24336569	KBM7	viability	TCCTCTGGGTTTGGAGGCAGAGG	PAK1	ENSG00000149269	0.3910654923416663	[833]	[2982]	3	hSpCas9	negative selection	2349660
77392394	77392417	11	+	24336569	KBM7	viability	GAGGCAGAGGTTTAGAACCATGG	PAK1	ENSG00000149269	0.4671070214436601	[282]	[1066]	4	hSpCas9	negative selection	2349661
77392400	77392423	11	+	24336569	KBM7	viability	GAGGTTTAGAACCATGGTTTAGG	PAK1	ENSG00000149269	0.6340190226382385	[231]	[981]	6	hSpCas9	negative selection	2349662
77392411	77392434	11	-	24336569	KBM7	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.3425185950742849	[416]	[896]	-3	hSpCas9	negative selection	2349663
77392426	77392449	11	-	24336569	KBM7	viability	GGAGCCGGCAGCAAAGATGCTGG	PAK1	ENSG00000149269	-0.44330200681389287	[841]	[1688]	-4	hSpCas9	negative selection	2349664
77392440	77392463	11	+	24336569	KBM7	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.5059424843934393	[275]	[1068]	4	hSpCas9	negative selection	2349665
77392447	77392470	11	-	24336569	KBM7	viability	AGAAATACCAGCACTATGATTGG	PAK1	ENSG00000149269	0.6593360073676293	[64]	[279]	6	hSpCas9	negative selection	2349666
77392453	77392476	11	+	24336569	KBM7	viability	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	-1.3116957684671098	[242]	[266]	-7	hSpCas9	negative selection	2349667
77392507	77392530	11	-	24336569	KBM7	viability	GTGGTGACAATGTCAAATAACGG	PAK1	ENSG00000149269	0.08804661812252162	[465]	[1350]	0	hSpCas9	negative selection	2349668
77334123	77334146	11	+	25494202	A375	resistance to PLX-4720 (puromycin)	CACACCATTGCACTCCAGCCTAG	PAK1	ENSG00000149269	1.262249387691805	[54,80]	[6,66]	5	dCas9-VP64	positive selection	2396323
77428311	77428334	11	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCCAGCACTTTGGGAGACCGAGG	PAK1	ENSG00000149269	0.3844762753670785	[5,31]	[5,4]	1	dCas9-VP64	positive selection	2432522
77346046	77346069	11	+	25494202	A375	resistance to PLX-4720 (puromycin)	TCACTGCAACCTCTGCCCCCAGG	PAK1	ENSG00000149269	-1.2513288674205318	[297,436]	[21,46]	-7	dCas9-VP64	positive selection	2446547
77387320	77387343	11	+	25494202	A375	resistance to PLX-4720 (puromycin)	GCCTCGGCCTCCCAAACTTCTGG	PAK1	ENSG00000149269	0.6798059408621834	[42,53]	[15,16]	3	dCas9-VP64	positive selection	2464727
77334123	77334146	11	+	25494202	A375	resistance to PLX-4720 (zeocin)	CACACCATTGCACTCCAGCCTAG	PAK1	ENSG00000149269	1.3472067688576927	[201,316]	[301,132]	7	dCas9-VP64	positive selection	2491578
77428311	77428334	11	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCCAGCACTTTGGGAGACCGAGG	PAK1	ENSG00000149269	0.9229581232819051	[29,251]	[8,157]	6	dCas9-VP64	positive selection	2527777
77346046	77346069	11	+	25494202	A375	resistance to PLX-4720 (zeocin)	TCACTGCAACCTCTGCCCCCAGG	PAK1	ENSG00000149269	0.4244343490827851	[184,278]	[65,133]	3	dCas9-VP64	positive selection	2541802
77387320	77387343	11	+	25494202	A375	resistance to PLX-4720 (zeocin)	GCCTCGGCCTCCCAAACTTCTGG	PAK1	ENSG00000149269	1.5849841440006536	[210,275]	[30,431]	8	dCas9-VP64	positive selection	2559982
77379299	77379322	11	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	0.33592381009565253	[3,3]	[2,5]	7	hSpCas9	positive selection	3009462
77392411	77392434	11	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.19131426249103306	[4,4]	[5,3]	4	hSpCas9	positive selection	3009463
77379299	77379322	11	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	0.7850224277190406	[3,3]	[5,3]	9	hSpCas9	positive selection	3077320
77392411	77392434	11	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.04829082166822313	[4,4]	[3,2]	0	hSpCas9	positive selection	3077321
77374351	77374374		-	24336571	A375	resistance to PLX after 7 days	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	0.4758164348097915	[18,21]	[27,28]	9	hSpCas9	positive selection	3133800
77379893	77379916		-	24336571	A375	resistance to PLX after 7 days	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.13248481076771124	[26,24]	[26,29]	4	hSpCas9	positive selection	3133801
77374327	77374350		-	24336571	A375	resistance to PLX after 7 days	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.09728727755123134	[18,19]	[21,19]	3	hSpCas9	positive selection	3133802
77379922	77379945		-	24336571	A375	resistance to PLX after 7 days	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	-0.08941107929206055	[10,9]	[9,8]	-3	hSpCas9	positive selection	3133803
77374351	77374374		-	24336571	A375	resistance to PLX after 14 days	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	1.0465039871080823	[16,13]	[13,15]	8	hSpCas9	positive selection	3191593
77379893	77379916		-	24336571	A375	resistance to PLX after 14 days	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.603142705359775	[24,29]	[38,39]	9	hSpCas9	positive selection	3191594
77374327	77374350		-	24336571	A375	resistance to PLX after 14 days	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.013454776173627725	[15,16]	[6,7]	0	hSpCas9	positive selection	3191595
77379922	77379945		-	24336571	A375	resistance to PLX after 14 days	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	0.867324707192944	[7,9]	[7,7]	7	hSpCas9	positive selection	3191596
77374351	77374374		-	24336571	A375	viability after 7 days	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	-0.010297818445103357	[21]	[18,21]	0	hSpCas9	negative selection	3249386
77379893	77379916		-	24336571	A375	viability after 7 days	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5980247810183473	[17]	[26,24]	8	hSpCas9	negative selection	3249387
77374327	77374350		-	24336571	A375	viability after 7 days	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.9834110806304381	[40]	[18,19]	-9	hSpCas9	negative selection	3249388
77379922	77379945		-	24336571	A375	viability after 7 days	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	0.43575493673491505	[7]	[10,9]	6	hSpCas9	negative selection	3249389
77374351	77374374		-	24336571	A375	viability after 14 days	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	-0.4053826128617666	[21]	[16,13]	-5	hSpCas9	negative selection	3307179
77379893	77379916		-	24336571	A375	viability after 14 days	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.692850437475483	[17]	[24,29]	8	hSpCas9	negative selection	3307180
77374327	77374350		-	24336571	A375	viability after 14 days	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-1.185839144036216	[40]	[15,16]	-9	hSpCas9	negative selection	3307181
77379922	77379945		-	24336571	A375	viability after 14 days	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	0.34517916913945546	[7]	[7,9]	4	hSpCas9	negative selection	3307182
77340645	77340668	11	-	27383988	293T	resistance to West Nile virus (flavivirus)	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.07741712143090065	[144,144]	[0,0]	0	hSpCas9	positive selection	3384509
77379893	77379916	11	-	27383988	293T	resistance to West Nile virus (flavivirus)	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.19123702098806272	[133,133]	[0,0]	1	hSpCas9	positive selection	3384510
77374327	77374350	11	-	27383988	293T	resistance to West Nile virus (flavivirus)	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.057653866609470694	[146,146]	[0,0]	0	hSpCas9	positive selection	3384511
77379298	77379321	11	+	26780180	HT29	viability (Avana library 4 designs)	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	1.09852473718561	[]	[]	4	hSpCas9	negative selection	3447497
77379969	77379992	11	-	26780180	HT29	viability (Avana library 4 designs)	ATAAAAAGAAAGAGAAAGAGCGG	PAK1	ENSG00000149269	3.27817936423179	[]	[]	8	hSpCas9	negative selection	3447498
77392453	77392476	11	+	26780180	HT29	viability (Avana library 4 designs)	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	0.00247617402378755	[]	[]	0	hSpCas9	negative selection	3447499
77392411	77392434	11	-	26780180	HT29	viability (Avana library 4 designs)	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	1.22199419184488	[]	[]	5	hSpCas9	negative selection	3447500
77392453	77392476	11	+	26780180	A375	viability (Avana lentiCRISPRv2)	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	-1.15954454157572	[]	[]	-1	hSpCas9	negative selection	3541333
77379969	77379992	11	-	26780180	A375	viability (Avana lentiCRISPRv2)	ATAAAAAGAAAGAGAAAGAGCGG	PAK1	ENSG00000149269	-16.9599683854397	[]	[]	-9	hSpCas9	negative selection	3541334
77379298	77379321	11	+	26780180	A375	viability (Avana lentiCRISPRv2)	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	-9.82924853239533	[]	[]	-7	hSpCas9	negative selection	3541335
77392411	77392434	11	-	26780180	A375	viability (Avana lentiCRISPRv2)	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-2.19241515647468	[]	[]	-3	hSpCas9	negative selection	3541336
77392453	77392476	11	+	26780180	A375	viability (Avana lentiGuide)	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	-2.55197128075986	[]	[]	-4	hSpCas9	negative selection	3649992
77379969	77379992	11	-	26780180	A375	viability (Avana lentiGuide)	ATAAAAAGAAAGAGAAAGAGCGG	PAK1	ENSG00000149269	2.56077316188904	[]	[]	2	hSpCas9	negative selection	3649993
77379298	77379321	11	+	26780180	A375	viability (Avana lentiGuide)	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	-1.45927907156305	[]	[]	-2	hSpCas9	negative selection	3649994
77392411	77392434	11	-	26780180	A375	viability (Avana lentiGuide)	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	5.49652845876663	[]	[]	5	hSpCas9	negative selection	3649995
77392453	77392476	11	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	-0.08670238754169557	[8]	[7,6]	-1	hSpCas9	positive selection	3758651
77379969	77379992	11	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ATAAAAAGAAAGAGAAAGAGCGG	PAK1	ENSG00000149269	-1.434577258458314	[8]	[3,0]	-9	hSpCas9	positive selection	3758652
77379298	77379321	11	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	-0.25685824344426034	[4]	[3,3]	-4	hSpCas9	positive selection	3758653
77392411	77392434	11	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.04591086890968335	[5]	[5,5]	1	hSpCas9	positive selection	3758654
77392453	77392476	11	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ATAGTGCTGGTATTTCTCATCGG	PAK1	ENSG00000149269	0.19731086210412002	[7]	[7,7]	5	hSpCas9	positive selection	3867258
77379969	77379992	11	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ATAAAAAGAAAGAGAAAGAGCGG	PAK1	ENSG00000149269	-1.9563458067711457	[3]	[0,0]	-9	hSpCas9	positive selection	3867259
77379298	77379321	11	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	ACACATCCAGAACAGCCTGCGGG	PAK1	ENSG00000149269	-0.6744833306703514	[5]	[0,4]	-7	hSpCas9	positive selection	3867260
77392411	77392434	11	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.10952739900657066	[6]	[5,5]	2	hSpCas9	positive selection	3867261
77340645	77340668	11	-	26780180	A375	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.951956891081638	[]	[]	4	hSpCas9	negative selection	3978153
77379240	77379263	11	-	26780180	A375	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.527739286027031	[]	[]	1	hSpCas9	negative selection	3978154
77379304	77379327	11	-	26780180	A375	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.521860855032904	[]	[]	1	hSpCas9	negative selection	3978155
77340752	77340775	11	-	26780180	A375	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	1.12186145032893	[]	[]	5	hSpCas9	negative selection	3978156
77379893	77379916	11	-	26780180	A375	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.433958567786459	[]	[]	1	hSpCas9	negative selection	3978157
77374327	77374350	11	-	26780180	A375	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	1.03854669322552	[]	[]	5	hSpCas9	negative selection	3978158
77340645	77340668	11	-	26780180	HT29	viability (GeCKOv2 library)	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.893636497962897	[]	[]	4	hSpCas9	negative selection	4086119
77379240	77379263	11	-	26780180	HT29	viability (GeCKOv2 library)	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	1.25978906618073	[]	[]	6	hSpCas9	negative selection	4086120
77379304	77379327	11	-	26780180	HT29	viability (GeCKOv2 library)	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.685247964568085	[]	[]	2	hSpCas9	negative selection	4086121
77340752	77340775	11	-	26780180	HT29	viability (GeCKOv2 library)	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.32680385047657	[]	[]	0	hSpCas9	negative selection	4086122
77379893	77379916	11	-	26780180	HT29	viability (GeCKOv2 library)	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.945487052067195	[]	[]	4	hSpCas9	negative selection	4086123
77374327	77374350	11	-	26780180	HT29	viability (GeCKOv2 library)	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	1.35994765948238	[]	[]	7	hSpCas9	negative selection	4086124
77374351	77374374	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	0.19249485662810858	[4]	[2,3]	5	hSpCas9	positive selection	4158647
77379893	77379916	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5993290789586451	[4]	[4,5]	9	hSpCas9	positive selection	4158648
77374327	77374350	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.03840103760268415	[4]	[2,2]	1	hSpCas9	positive selection	4158649
77379922	77379945	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	0.16694550137030978	[3]	[2,2]	4	hSpCas9	positive selection	4158650
77374351	77374374	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CTGTGCAGATAAGTCAGCTGAGG	PAK1	ENSG00000149269	1.050147567130323	[4]	[5,2,4,3]	7	hSpCas9	positive selection	4222669
77379893	77379916	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.1728637559662685	[3]	[3,2,3,3]	8	hSpCas9	positive selection	4222670
77374327	77374350	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.7272890065550468	[3]	[1,0,0,0]	-8	hSpCas9	positive selection	4222671
77379922	77379945	11	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TTTGAACACACAATTCATGTCGG	PAK1	ENSG00000149269	0.9326434395571334	[5]	[3,4,5,3]	6	hSpCas9	positive selection	4222672
77340752	77340775	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.2188081401829381	[4]	[4,3]	4	hSpCas9	positive selection	4288686
77379304	77379327	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	-0.049811570628005564	[0]	[0,0]	-1	hSpCas9	positive selection	4288687
77379240	77379263	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.014918906755854278	[1]	[2,0]	0	hSpCas9	positive selection	4288688
77340645	77340668	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.1692474202694025	[2]	[3,0]	-3	hSpCas9	positive selection	4352939
77379893	77379916	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.16000028155649573	[2]	[1,1]	3	hSpCas9	positive selection	4352940
77374327	77374350	11	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.13377843655040517	[3]	[3,0]	-3	hSpCas9	positive selection	4352941
77379903	77379925	11	-	27760321	OCIAML3	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.569890679537107	[639,636]	[733,755]	6	hSpCas9	negative selection	4425389
77392344	77392366	11	+	27760321	OCIAML3	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-3.2987000956212995	[11,12]	[0,0]	-9	hSpCas9	negative selection	4425390
77392411	77392433	11	-	27760321	OCIAML3	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.0016239281075548506	[343,248]	[280,161]	0	hSpCas9	negative selection	4425391
77355725	77355747	11	+	27760321	OCIAML3	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.24192913243286943	[558,459]	[366,601]	3	hSpCas9	negative selection	4425392
77355782	77355804	11	+	27760321	OCIAML3	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.030583336203612088	[869,835]	[718,634]	0	hSpCas9	negative selection	4425393
77379903	77379925	11	-	27760321	OCIAML2	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.17953517936736074	[639,636]	[493,708]	2	hSpCas9	negative selection	4508852
77392344	77392366	11	+	27760321	OCIAML2	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	1.2176763740597338	[11,12]	[8,42]	9	hSpCas9	negative selection	4508853
77392411	77392433	11	-	27760321	OCIAML2	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.07242403253725815	[343,248]	[224,226]	-1	hSpCas9	negative selection	4508854
77355725	77355747	11	+	27760321	OCIAML2	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	-0.18224119130228672	[558,459]	[301,439]	-2	hSpCas9	negative selection	4508855
77355782	77355804	11	+	27760321	OCIAML2	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.14440399010009652	[869,835]	[646,917]	2	hSpCas9	negative selection	4508856
77379903	77379925	11	-	27760321	MV411	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-0.5908588126656915	[639,636]	[300,249]	-4	hSpCas9	negative selection	4592315
77392344	77392366	11	+	27760321	MV411	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-3.0536215267066176	[11,12]	[0,0]	-9	hSpCas9	negative selection	4592316
77392411	77392433	11	-	27760321	MV411	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-1.4696132064141105	[343,248]	[11,156]	-7	hSpCas9	negative selection	4592317
77355725	77355747	11	+	27760321	MV411	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.6559591924034237	[558,459]	[627,375]	5	hSpCas9	negative selection	4592318
77355782	77355804	11	+	27760321	MV411	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.4291265201511054	[869,835]	[126,856]	-3	hSpCas9	negative selection	4592319
77379903	77379925	11	-	27760321	HL60	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.678423046388771	[639,636]	[863,808]	7	hSpCas9	negative selection	4675778
77392344	77392366	11	+	27760321	HL60	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	1.740808010183664	[11,12]	[45,21]	9	hSpCas9	negative selection	4675779
77392411	77392433	11	-	27760321	HL60	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.7207543132392927	[343,248]	[145,144]	-6	hSpCas9	negative selection	4675780
77355725	77355747	11	+	27760321	HL60	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	-0.37767180090916086	[558,459]	[329,306]	-4	hSpCas9	negative selection	4675781
77355782	77355804	11	+	27760321	HL60	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.20447272631877922	[869,835]	[645,562]	-2	hSpCas9	negative selection	4675782
77379903	77379925	11	-	27760321	HT1080	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-3.7769474775155474	[636,496]	[15,16]	-8	hSpCas9	negative selection	4759241
77392344	77392366	11	+	27760321	HT1080	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	5.17925543149324	[12,9]	[130,203]	9	hSpCas9	negative selection	4759242
77392411	77392433	11	-	27760321	HT1080	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	1.1996945655615314	[248,194]	[90,325]	6	hSpCas9	negative selection	4759243
77355725	77355747	11	+	27760321	HT1080	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	1.6070307907380164	[459,358]	[442,555]	8	hSpCas9	negative selection	4759244
77355782	77355804	11	+	27760321	HT1080	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-8.212383831329907	[835,652]	[0,0]	-9	hSpCas9	negative selection	4759245
77379903	77379925	11	-	27760321	HT29	viability after 7 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-0.42168036713369267	[636,496]	[527,538,561]	-6	hSpCas9	negative selection	4842704
77392344	77392366	11	+	27760321	HT29	viability after 7 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-1.4999235821786359	[12,9]	[7,0,6]	-9	hSpCas9	negative selection	4842705
77392411	77392433	11	-	27760321	HT29	viability after 7 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.18334248235569128	[248,194]	[352,310,108]	-3	hSpCas9	negative selection	4842706
77355725	77355747	11	+	27760321	HT29	viability after 7 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.09192123200901214	[459,358]	[461,598,605]	1	hSpCas9	negative selection	4842707
77355782	77355804	11	+	27760321	HT29	viability after 7 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.0213273891177006	[835,652]	[1294,848,740]	0	hSpCas9	negative selection	4842708
77379903	77379925	11	-	27760321	HT29	viability after 25 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-0.007984964569361597	[636,496]	[486,647,825]	0	hSpCas9	negative selection	4926167
77392344	77392366	11	+	27760321	HT29	viability after 25 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-3.714133241048061	[12,9]	[0,0,0]	-9	hSpCas9	negative selection	4926168
77392411	77392433	11	-	27760321	HT29	viability after 25 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.10953297297413611	[248,194]	[223,280,196]	-1	hSpCas9	negative selection	4926169
77355725	77355747	11	+	27760321	HT29	viability after 25 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	-0.6190587188982459	[459,358]	[354,297,251]	-5	hSpCas9	negative selection	4926170
77355782	77355804	11	+	27760321	HT29	viability after 25 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.30869675846030964	[835,652]	[611,675,786]	-3	hSpCas9	negative selection	4926171
77379903	77379925	11	-	27760321	HT29	viability after 22 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-0.092739822466957	[636,496]	[755,482,600]	-1	hSpCas9	negative selection	5009630
77392344	77392366	11	+	27760321	HT29	viability after 22 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-3.7314518729073205	[12,9]	[0,0,0]	-9	hSpCas9	negative selection	5009631
77392411	77392433	11	-	27760321	HT29	viability after 22 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.014671256954391643	[248,194]	[369,255,131]	0	hSpCas9	negative selection	5009632
77355725	77355747	11	+	27760321	HT29	viability after 22 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	-0.16002220929041264	[459,358]	[349,506,415]	-2	hSpCas9	negative selection	5009633
77355782	77355804	11	+	27760321	HT29	viability after 22 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.16476730250362193	[835,652]	[824,698,777]	-2	hSpCas9	negative selection	5009634
77379903	77379925	11	-	27760321	HT29	viability after 19 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.24333625751055954	[636,496]	[810,706,787]	2	hSpCas9	negative selection	5093093
77392344	77392366	11	+	27760321	HT29	viability after 19 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-1.1592234805892874	[12,9]	[16,0,0]	-7	hSpCas9	negative selection	5093094
77392411	77392433	11	-	27760321	HT29	viability after 19 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.20171668224625827	[248,194]	[248,188,226]	-2	hSpCas9	negative selection	5093095
77355725	77355747	11	+	27760321	HT29	viability after 19 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.08000320296027774	[459,358]	[418,915,154]	0	hSpCas9	negative selection	5093096
77355782	77355804	11	+	27760321	HT29	viability after 19 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.07618214074868179	[835,652]	[1079,962,422]	0	hSpCas9	negative selection	5093097
77379903	77379925	11	-	27760321	HT29	viability after 16 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.056644745137928054	[636,496]	[505,721,734]	0	hSpCas9	negative selection	5176556
77392344	77392366	11	+	27760321	HT29	viability after 16 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-1.3485249251195421	[12,9]	[12,0,1]	-7	hSpCas9	negative selection	5176557
77392411	77392433	11	-	27760321	HT29	viability after 16 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.6115601540517788	[248,194]	[235,73,186]	-5	hSpCas9	negative selection	5176558
77355725	77355747	11	+	27760321	HT29	viability after 16 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	-0.00429336689907206	[459,358]	[390,497,473]	0	hSpCas9	negative selection	5176559
77355782	77355804	11	+	27760321	HT29	viability after 16 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.05663385494263129	[835,652]	[845,873,684]	0	hSpCas9	negative selection	5176560
77379903	77379925	11	-	27760321	HT29	viability after 13 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	0.07647106004815052	[636,496]	[824,573,681]	0	hSpCas9	negative selection	5260019
77392344	77392366	11	+	27760321	HT29	viability after 13 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	0.7930378019773521	[12,9]	[39,1,26]	9	hSpCas9	negative selection	5260020
77392411	77392433	11	-	27760321	HT29	viability after 13 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.006903147132055754	[248,194]	[290,204,271]	0	hSpCas9	negative selection	5260021
77355725	77355747	11	+	27760321	HT29	viability after 13 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.1208735079454083	[459,358]	[735,532,293]	1	hSpCas9	negative selection	5260022
77355782	77355804	11	+	27760321	HT29	viability after 13 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.05799490061877277	[835,652]	[759,992,941]	0	hSpCas9	negative selection	5260023
77379903	77379925	11	-	27760321	HT29	viability after 10 days	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	-0.12320798360000207	[636,496]	[599,535,806]	-2	hSpCas9	negative selection	5343482
77392344	77392366	11	+	27760321	HT29	viability after 10 days	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	-0.8732314808023949	[12,9]	[0,0,20]	-7	hSpCas9	negative selection	5343483
77392411	77392433	11	-	27760321	HT29	viability after 10 days	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.13186440943339528	[248,194]	[496,211,201]	1	hSpCas9	negative selection	5343484
77355725	77355747	11	+	27760321	HT29	viability after 10 days	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.16445672569069353	[459,358]	[760,569,391]	2	hSpCas9	negative selection	5343485
77355782	77355804	11	+	27760321	HT29	viability after 10 days	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.026587747934027517	[835,652]	[945,702,1078]	0	hSpCas9	negative selection	5343486
77379903	77379925	11	-	27760321	MOLM13	viability	CGGTTTTGATGCTGTCACAGGG	PAK1	ENSG00000149269	1.1047715878102942	[639,636]	[976,606]	7	hSpCas9	negative selection	5426945
77392344	77392366	11	+	27760321	MOLM13	viability	TAAAATGGATCGGTAAAATCGG	PAK1	ENSG00000149269	3.491898845846861	[11,12]	[0,146]	9	hSpCas9	negative selection	5426946
77392411	77392433	11	-	27760321	MOLM13	viability	ATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.5532149587501892	[343,248]	[302,191]	4	hSpCas9	negative selection	5426947
77355725	77355747	11	+	27760321	MOLM13	viability	TATTCCGGGTCAAAGCATCTGG	PAK1	ENSG00000149269	0.28810758120904634	[558,459]	[384,318]	2	hSpCas9	negative selection	5426948
77355782	77355804	11	+	27760321	MOLM13	viability	ATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.2455886463282253	[869,835]	[485,338]	-2	hSpCas9	negative selection	5426949
77340752	77340775	11	-	27260156	BXPC3	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.0028278044800782443	[1566]	[1761,1388,1558,1873]	0	hSpCas9	negative selection	5561146
77379304	77379327	11	-	27260156	BXPC3	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.8657376063386324	[39]	[80,66,66,90]	9	hSpCas9	negative selection	5561147
77379240	77379263	11	-	27260156	BXPC3	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.3528824384513942	[104]	[159,136,82,181]	7	hSpCas9	negative selection	5561148
77340645	77340668	11	-	27260156	BXPC3	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.18938682186794198	[423]	[398,341,380,437]	-4	hSpCas9	negative selection	5625399
77379893	77379916	11	-	27260156	BXPC3	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.43899647696292987	[253]	[283,330,397,445]	8	hSpCas9	negative selection	5625400
77374327	77374350	11	-	27260156	BXPC3	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.05497055424366187	[500]	[468,485,463,611]	-1	hSpCas9	negative selection	5625401
77340752	77340775	11	-	27260156	A673	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.08848561575144998	[1566]	[2192,1859,1844,2134]	2	hSpCas9	negative selection	5682397
77379304	77379327	11	-	27260156	A673	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.7751845210926147	[39]	[89,91,85,59]	9	hSpCas9	negative selection	5682398
77379240	77379263	11	-	27260156	A673	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.05789971880573441	[104]	[123,89,171,138]	1	hSpCas9	negative selection	5682399
77340645	77340668	11	-	27260156	A673	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.024871998436908377	[423]	[512,453,575,528]	0	hSpCas9	negative selection	5746650
77379893	77379916	11	-	27260156	A673	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.2928276846860467	[253]	[442,321,366,370]	7	hSpCas9	negative selection	5746651
77374327	77374350	11	-	27260156	A673	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.13214701322584793	[500]	[631,722,577,700]	3	hSpCas9	negative selection	5746652
77340752	77340775	11	-	27260156	A375	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.33459792232401675	[1566]	[1207,1777,1502,1364]	-5	hSpCas9	negative selection	5803648
77379304	77379327	11	-	27260156	A375	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.1643387752811445	[39]	[94,85,85,143]	9	hSpCas9	negative selection	5803649
77379240	77379263	11	-	27260156	A375	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.25505814157706386	[104]	[56,278,215,60]	4	hSpCas9	negative selection	5803650
77340645	77340668	11	-	27260156	A375	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.5379493110834203	[423]	[357,340,404,267]	-6	hSpCas9	negative selection	5867901
77379893	77379916	11	-	27260156	A375	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.7699970885433641	[253]	[656,439,386,526]	9	hSpCas9	negative selection	5867902
77374327	77374350	11	-	27260156	A375	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.2538857872481784	[500]	[804,610,736,631]	3	hSpCas9	negative selection	5867903
77340752	77340775	11	-	27260156	COLO741	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.022319257912966295	[1566]	[2235,2102,1791]	0	hSpCas9	negative selection	5924899
77379304	77379327	11	-	27260156	COLO741	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.48243513623588763	[39]	[88,61,64]	8	hSpCas9	negative selection	5924900
77379240	77379263	11	-	27260156	COLO741	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	1.2364169690653783	[104]	[129,176,602]	9	hSpCas9	negative selection	5924901
77340645	77340668	11	-	27260156	COLO741	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.2791652814424758	[423]	[510,418,415]	-5	hSpCas9	negative selection	5989152
77379893	77379916	11	-	27260156	COLO741	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.21334990612211147	[253]	[332,423,367]	4	hSpCas9	negative selection	5989153
77374327	77374350	11	-	27260156	COLO741	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.27336229345713453	[500]	[774,597,933]	6	hSpCas9	negative selection	5989154
77340752	77340775	11	-	27260156	CAL120	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.34469791065265776	[1566]	[1669,1632,1326]	-5	hSpCas9	negative selection	6046150
77379304	77379327	11	-	27260156	CAL120	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.0009265691902676	[39]	[74,58,159]	9	hSpCas9	negative selection	6046151
77379240	77379263	11	-	27260156	CAL120	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.7391756226108397	[104]	[290,89,282]	9	hSpCas9	negative selection	6046152
77340645	77340668	11	-	27260156	CAL120	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.03243736342247927	[423]	[506,348,686]	0	hSpCas9	negative selection	6110403
77379893	77379916	11	-	27260156	CAL120	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.1191042980056758	[253]	[792,741,542]	9	hSpCas9	negative selection	6110404
77374327	77374350	11	-	27260156	CAL120	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.30296562889085776	[500]	[655,1142,495]	4	hSpCas9	negative selection	6110405
77340752	77340775	11	-	27260156	CADOES1	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.13177875515831716	[1566]	[1119,1312,1561,1325]	-2	hSpCas9	negative selection	6167401
77379304	77379327	11	-	27260156	CADOES1	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.0317741844418382	[39]	[72,80,29,121]	9	hSpCas9	negative selection	6167402
77379240	77379263	11	-	27260156	CADOES1	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.4821219624212063	[104]	[165,105,72,202]	7	hSpCas9	negative selection	6167403
77340645	77340668	11	-	27260156	CADOES1	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.10771652398871545	[423]	[313,299,403,448]	-2	hSpCas9	negative selection	6231654
77379893	77379916	11	-	27260156	CADOES1	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.7654797793578637	[253]	[469,483,361,285]	9	hSpCas9	negative selection	6231655
77374327	77374350	11	-	27260156	CADOES1	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.14753003946010168	[500]	[666,469,437,487]	2	hSpCas9	negative selection	6231656
77340752	77340775	11	-	27260156	EWS502	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.07097736694898082	[1566]	[1490,1535,1763,1534]	-1	hSpCas9	negative selection	6288652
77379304	77379327	11	-	27260156	EWS502	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.40044007367457723	[39]	[82,43,27,65]	7	hSpCas9	negative selection	6288653
77379240	77379263	11	-	27260156	EWS502	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.3048189121157081	[104]	[122,256,47,114]	5	hSpCas9	negative selection	6288654
77340645	77340668	11	-	27260156	EWS502	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.08023298969414999	[423]	[493,570,465,350]	1	hSpCas9	negative selection	6352905
77379893	77379916	11	-	27260156	EWS502	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.6001258371318785	[253]	[343,407,323,571]	9	hSpCas9	negative selection	6352906
77374327	77374350	11	-	27260156	EWS502	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.07180584394434723	[500]	[684,446,533,550]	1	hSpCas9	negative selection	6352907
77340752	77340775	11	-	27260156	EW8	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.17765125341937882	[1566]	[1161,915,1222,1172]	-3	hSpCas9	negative selection	6409903
77379304	77379327	11	-	27260156	EW8	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.8050308635775445	[39]	[46,25,62,87]	9	hSpCas9	negative selection	6409904
77379240	77379263	11	-	27260156	EW8	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.5534429209483347	[104]	[157,96,104,145]	8	hSpCas9	negative selection	6409905
77340645	77340668	11	-	27260156	EW8	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.30137976615989964	[423]	[251,303,234,327]	-5	hSpCas9	negative selection	6474156
77379893	77379916	11	-	27260156	EW8	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5268109277248334	[253]	[338,257,319,268]	8	hSpCas9	negative selection	6474157
77374327	77374350	11	-	27260156	EW8	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.1206323956468459	[500]	[420,352,395,598]	2	hSpCas9	negative selection	6474158
77340752	77340775	11	-	27260156	CORL105	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.16204128772309034	[1566]	[1557,1711,1611,1666]	-3	hSpCas9	negative selection	6531154
77379304	77379327	11	-	27260156	CORL105	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.225408579727516	[39]	[74,70,126,166]	9	hSpCas9	negative selection	6531155
77379240	77379263	11	-	27260156	CORL105	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.2230457240976964	[104]	[95,79,109,135]	-4	hSpCas9	negative selection	6531156
77340645	77340668	11	-	27260156	CORL105	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.15437565898634303	[423]	[437,391,674,315]	-3	hSpCas9	negative selection	6595407
77379893	77379916	11	-	27260156	CORL105	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.7635202559283499	[253]	[488,526,466,528]	9	hSpCas9	negative selection	6595408
77374327	77374350	11	-	27260156	CORL105	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.26361865018157826	[500]	[398,424,540,594]	-5	hSpCas9	negative selection	6595409
77340752	77340775	11	-	27260156	HS294T	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.182324003267053	[1566]	[460,392,527,809]	-3	hSpCas9	negative selection	6652405
77379304	77379327	11	-	27260156	HS294T	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.3981423883704283	[39]	[27,89,20,19]	9	hSpCas9	negative selection	6652406
77379240	77379263	11	-	27260156	HS294T	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.4268367125908368	[104]	[44,62,34,79]	6	hSpCas9	negative selection	6652407
77340645	77340668	11	-	27260156	HS294T	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.3505877984576655	[423]	[145,110,115,141]	-5	hSpCas9	negative selection	6716658
77379893	77379916	11	-	27260156	HS294T	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.8962917196781324	[253]	[214,195,117,200]	9	hSpCas9	negative selection	6716659
77374327	77374350	11	-	27260156	HS294T	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.2478743004033629	[500]	[223,153,136,124]	-3	hSpCas9	negative selection	6716660
77340752	77340775	11	-	27260156	HCC44	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.05460665727912217	[1566]	[2002,1413,1942,1565]	-1	hSpCas9	negative selection	6773656
77379304	77379327	11	-	27260156	HCC44	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.6784863773232601	[39]	[100,41,113,46]	9	hSpCas9	negative selection	6773657
77379240	77379263	11	-	27260156	HCC44	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.23976389791008423	[104]	[76,133,60,264]	4	hSpCas9	negative selection	6773658
77340645	77340668	11	-	27260156	HCC44	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.08012223679366454	[423]	[378,379,680,414]	-1	hSpCas9	negative selection	6837909
77379893	77379916	11	-	27260156	HCC44	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.1289165393757573	[253]	[326,278,352,307]	2	hSpCas9	negative selection	6837910
77374327	77374350	11	-	27260156	HCC44	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.2181264122970556	[500]	[587,607,598,820]	3	hSpCas9	negative selection	6837911
77340752	77340775	11	-	27260156	G402	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.2323221156901636	[1566]	[1439,991,2183,1814]	-3	hSpCas9	negative selection	6894907
77379304	77379327	11	-	27260156	G402	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.22934559857553216	[39]	[34,159,33,4]	3	hSpCas9	negative selection	6894908
77379240	77379263	11	-	27260156	G402	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.07396171009236996	[104]	[154,201,101,88]	0	hSpCas9	negative selection	6894909
77340645	77340668	11	-	27260156	G402	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.030922575595273033	[423]	[502,371,690,451]	0	hSpCas9	negative selection	6959160
77379893	77379916	11	-	27260156	G402	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5060471192230553	[253]	[287,905,235,338]	6	hSpCas9	negative selection	6959161
77374327	77374350	11	-	27260156	G402	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.07691209902095812	[500]	[853,603,337,561]	-1	hSpCas9	negative selection	6959162
77340752	77340775	11	-	27260156	L33	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.0014806541415481878	[1566]	[1606,696,943,1463]	0	hSpCas9	negative selection	7016158
77379304	77379327	11	-	27260156	L33	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.12670269337701434	[39]	[30,37,6,39]	3	hSpCas9	negative selection	7016159
77379240	77379263	11	-	27260156	L33	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.3550532724039934	[104]	[110,47,84,173]	7	hSpCas9	negative selection	7016160
77340645	77340668	11	-	27260156	L33	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.08898993142907083	[423]	[358,214,217,364]	-2	hSpCas9	negative selection	7080411
77379893	77379916	11	-	27260156	L33	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.31877168048037274	[253]	[348,125,227,247]	6	hSpCas9	negative selection	7080412
77374327	77374350	11	-	27260156	L33	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.1972667803926415	[500]	[457,182,259,428]	-4	hSpCas9	negative selection	7080413
77340752	77340775	11	-	27260156	K562	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.7860921260855067	[1566]	[971,769,959,560]	-7	hSpCas9	negative selection	7137409
77379304	77379327	11	-	27260156	K562	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	-0.5622209847500867	[39]	[29,8,34,21]	-6	hSpCas9	negative selection	7137410
77379240	77379263	11	-	27260156	K562	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.3912843032063671	[104]	[105,146,81,149]	5	hSpCas9	negative selection	7137411
77340645	77340668	11	-	27260156	K562	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.4984355393152119	[423]	[480,405,103,115]	-5	hSpCas9	negative selection	7201662
77379893	77379916	11	-	27260156	K562	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.1184225559585579	[253]	[649,595,472,279]	9	hSpCas9	negative selection	7201663
77374327	77374350	11	-	27260156	K562	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.28872176327187216	[500]	[375,911,506,397]	3	hSpCas9	negative selection	7201664
77340752	77340775	11	-	27260156	HT29	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.04257339583521458	[1566]	[2183,1955,2089,1657]	0	hSpCas9	negative selection	7258660
77379304	77379327	11	-	27260156	HT29	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.5157033602270307	[39]	[73,66,51,82]	8	hSpCas9	negative selection	7258661
77379240	77379263	11	-	27260156	HT29	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.09333778586889441	[104]	[114,106,221,41]	-2	hSpCas9	negative selection	7258662
77340645	77340668	11	-	27260156	HT29	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.02130300312215927	[423]	[571,525,663,351]	0	hSpCas9	negative selection	7322913
77379893	77379916	11	-	27260156	HT29	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.40473636921889994	[253]	[334,389,510,390]	7	hSpCas9	negative selection	7322914
77374327	77374350	11	-	27260156	HT29	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.10544217801676449	[500]	[841,580,787,439]	2	hSpCas9	negative selection	7322915
77340752	77340775	11	-	27260156	MHHES1	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.19853656934213793	[1566]	[1978,1708,1347,1223]	-4	hSpCas9	negative selection	7379911
77379304	77379327	11	-	27260156	MHHES1	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.1261891117917706	[39]	[127,119,82,71]	9	hSpCas9	negative selection	7379912
77379240	77379263	11	-	27260156	MHHES1	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.026959292802892776	[104]	[182,98,125,84]	0	hSpCas9	negative selection	7379913
77340645	77340668	11	-	27260156	MHHES1	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.14951709436484717	[423]	[540,535,349,331]	-3	hSpCas9	negative selection	7444164
77379893	77379916	11	-	27260156	MHHES1	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.057027702398239066	[253]	[257,354,266,302]	1	hSpCas9	negative selection	7444165
77374327	77374350	11	-	27260156	MHHES1	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.2532658925325701	[500]	[626,407,476,399]	-5	hSpCas9	negative selection	7444166
77340752	77340775	11	-	27260156	MEWO	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.1627377197931914	[1566]	[1534,1369,1066]	-4	hSpCas9	negative selection	7501162
77379304	77379327	11	-	27260156	MEWO	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.778579000719035	[39]	[92,187,96]	9	hSpCas9	negative selection	7501163
77379240	77379263	11	-	27260156	MEWO	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.24954457096322136	[104]	[109,118,119]	5	hSpCas9	negative selection	7501164
77340645	77340668	11	-	27260156	MEWO	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.4156536655346529	[423]	[336,246,315]	-7	hSpCas9	negative selection	7565415
77379893	77379916	11	-	27260156	MEWO	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.4647145678403158	[253]	[551,156,318]	8	hSpCas9	negative selection	7565416
77374327	77374350	11	-	27260156	MEWO	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.003274452316832835	[500]	[598,324,504]	0	hSpCas9	negative selection	7565417
77340752	77340775	11	-	27260156	LNCAPCLONEFGC	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.21568409236724628	[1566]	[1384,1119,1449,970]	-3	hSpCas9	negative selection	7622413
77379304	77379327	11	-	27260156	LNCAPCLONEFGC	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.5769844530354498	[39]	[148,63,104,115]	9	hSpCas9	negative selection	7622414
77379240	77379263	11	-	27260156	LNCAPCLONEFGC	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.026652429270424882	[104]	[50,119,116,87]	0	hSpCas9	negative selection	7622415
77340645	77340668	11	-	27260156	LNCAPCLONEFGC	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.08345227562964563	[423]	[434,278,376,364]	-1	hSpCas9	negative selection	7686666
77379893	77379916	11	-	27260156	LNCAPCLONEFGC	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.8124637038821518	[253]	[410,361,486,370]	9	hSpCas9	negative selection	7686667
77374327	77374350	11	-	27260156	LNCAPCLONEFGC	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.16381423560618735	[500]	[421,384,396,415]	-3	hSpCas9	negative selection	7686668
77340752	77340775	11	-	27260156	PANC0327	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.07611592929067523	[1566]	[1414,1715,1144,961]	-1	hSpCas9	negative selection	7743664
77379304	77379327	11	-	27260156	PANC0327	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	-0.23148111968155582	[39]	[21,25,65,6]	-3	hSpCas9	negative selection	7743665
77379240	77379263	11	-	27260156	PANC0327	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.2850866181421621	[104]	[105,105,147,94]	4	hSpCas9	negative selection	7743666
77340645	77340668	11	-	27260156	PANC0327	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.37869030832488654	[423]	[570,392,589,423]	5	hSpCas9	negative selection	7807917
77379893	77379916	11	-	27260156	PANC0327	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5236907719787542	[253]	[286,488,249,246]	7	hSpCas9	negative selection	7807918
77374327	77374350	11	-	27260156	PANC0327	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.20722013996300492	[500]	[531,632,434,440]	3	hSpCas9	negative selection	7807919
77340752	77340775	11	-	27260156	NCIH2009	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.006761248742978676	[1566]	[2433,1903,2250]	0	hSpCas9	negative selection	7864915
77379304	77379327	11	-	27260156	NCIH2009	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.2598617652792563	[39]	[114,163,117]	9	hSpCas9	negative selection	7864916
77379240	77379263	11	-	27260156	NCIH2009	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.7198620015887589	[104]	[339,235,167]	8	hSpCas9	negative selection	7864917
77340645	77340668	11	-	27260156	NCIH2009	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.009220261446950012	[423]	[644,662,487]	0	hSpCas9	negative selection	7929168
77379893	77379916	11	-	27260156	NCIH2009	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.8135395220714536	[253]	[699,575,609]	9	hSpCas9	negative selection	7929169
77374327	77374350	11	-	27260156	NCIH2009	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.18964237836493003	[500]	[687,552,614]	-3	hSpCas9	negative selection	7929170
77340752	77340775	11	-	27260156	NCIH1373	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.33973704069908983	[1566]	[1738,1169,1164,410]	-3	hSpCas9	negative selection	7986166
77379304	77379327	11	-	27260156	NCIH1373	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.7036100959905749	[39]	[82,61,41,28]	7	hSpCas9	negative selection	7986167
77379240	77379263	11	-	27260156	NCIH1373	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	1.1483954602814792	[104]	[261,247,312,56]	9	hSpCas9	negative selection	7986168
77340645	77340668	11	-	27260156	NCIH1373	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.3250143021938221	[423]	[719,264,257,324]	3	hSpCas9	negative selection	8050419
77379893	77379916	11	-	27260156	NCIH1373	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.4755009337340448	[253]	[567,1001,1037,123]	9	hSpCas9	negative selection	8050420
77374327	77374350	11	-	27260156	NCIH1373	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.5608496557812492	[500]	[571,268,439,71]	-5	hSpCas9	negative selection	8050421
77340752	77340775	11	-	27260156	PATU8902	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.29582490037855225	[1566]	[1470,2472,1494,1731]	-3	hSpCas9	negative selection	8107417
77379304	77379327	11	-	27260156	PATU8902	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.9454942283917429	[39]	[273,126,26,6]	8	hSpCas9	negative selection	8107418
77379240	77379263	11	-	27260156	PATU8902	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.7195145118638566	[104]	[73,462,267,139]	7	hSpCas9	negative selection	8107419
77340645	77340668	11	-	27260156	PATU8902	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.2127982031208815	[423]	[401,508,276,961]	-2	hSpCas9	negative selection	8171670
77379893	77379916	11	-	27260156	PATU8902	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.7876801251119394	[253]	[491,880,478,623]	8	hSpCas9	negative selection	8171671
77374327	77374350	11	-	27260156	PATU8902	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.25945323783894825	[500]	[520,812,617,337]	-3	hSpCas9	negative selection	8171672
77340752	77340775	11	-	27260156	PANC1	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.12662565353722588	[1566]	[1841,1835,1255,1053]	2	hSpCas9	negative selection	8228668
77379304	77379327	11	-	27260156	PANC1	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.7925853854502374	[39]	[73,136,40,8]	9	hSpCas9	negative selection	8228669
77379240	77379263	11	-	27260156	PANC1	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.4804273541917059	[104]	[89,97,34,43]	-6	hSpCas9	negative selection	8228670
77340645	77340668	11	-	27260156	PANC1	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.5011323799617228	[423]	[266,344,218,217]	-6	hSpCas9	negative selection	8292921
77379893	77379916	11	-	27260156	PANC1	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.363226392186369	[253]	[354,286,281,205]	6	hSpCas9	negative selection	8292922
77374327	77374350	11	-	27260156	PANC1	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.26384803545469915	[500]	[446,497,453,635]	4	hSpCas9	negative selection	8292923
77340752	77340775	11	-	27260156	PANC0813	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.0705015173507737	[1566]	[2142,2066,1758,1785]	1	hSpCas9	negative selection	8349919
77379304	77379327	11	-	27260156	PANC0813	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.6492209675895109	[39]	[71,20,131,69]	8	hSpCas9	negative selection	8349920
77379240	77379263	11	-	27260156	PANC0813	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.5478867804121923	[104]	[165,156,253,144]	8	hSpCas9	negative selection	8349921
77340645	77340668	11	-	27260156	PANC0813	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.00537376169784598	[423]	[730,450,345,477]	0	hSpCas9	negative selection	8414172
77379893	77379916	11	-	27260156	PANC0813	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5249581576659115	[253]	[334,545,318,502]	8	hSpCas9	negative selection	8414173
77374327	77374350	11	-	27260156	PANC0813	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.21820506807612883	[500]	[641,498,460,436]	-3	hSpCas9	negative selection	8414174
77340752	77340775	11	-	27260156	RDES	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.2818342728624099	[1566]	[1229,1389,1691,1671]	-5	hSpCas9	negative selection	8471170
77379304	77379327	11	-	27260156	RDES	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.3117592469805675	[39]	[70,92,168,137]	9	hSpCas9	negative selection	8471171
77379240	77379263	11	-	27260156	RDES	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.3256615679408006	[104]	[79,132,243,167]	5	hSpCas9	negative selection	8471172
77340645	77340668	11	-	27260156	RDES	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.28889869593361645	[423]	[266,332,451,601]	-5	hSpCas9	negative selection	8535423
77379893	77379916	11	-	27260156	RDES	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.7995512330024178	[253]	[443,398,637,576]	9	hSpCas9	negative selection	8535424
77374327	77374350	11	-	27260156	RDES	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.022765364375735772	[500]	[548,527,601,669]	0	hSpCas9	negative selection	8535425
77340752	77340775	11	-	27260156	PC3	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.14124723412778972	[1566]	[858,1357,2476,1501]	-3	hSpCas9	negative selection	8592421
77379304	77379327	11	-	27260156	PC3	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.9035208416592533	[39]	[6,132,140,66]	9	hSpCas9	negative selection	8592422
77379240	77379263	11	-	27260156	PC3	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.017191745591674135	[104]	[50,124,108,145]	0	hSpCas9	negative selection	8592423
77340645	77340668	11	-	27260156	PC3	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.1699050123100463	[423]	[213,434,645,344]	-4	hSpCas9	negative selection	8656674
77379893	77379916	11	-	27260156	PC3	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.2696189206139209	[253]	[174,224,666,330]	6	hSpCas9	negative selection	8656675
77374327	77374350	11	-	27260156	PC3	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.10812594410554087	[500]	[252,458,1171,608]	2	hSpCas9	negative selection	8656676
77340752	77340775	11	-	27260156	PATU8988T	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.17875692109330643	[1566]	[1548,1550,1912,1833]	3	hSpCas9	negative selection	8713672
77379304	77379327	11	-	27260156	PATU8988T	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	-0.3148252392340054	[39]	[19,19,31,52]	-5	hSpCas9	negative selection	8713673
77379240	77379263	11	-	27260156	PATU8988T	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.013474758648098106	[104]	[75,156,41,135]	0	hSpCas9	negative selection	8713674
77340645	77340668	11	-	27260156	PATU8988T	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.3913500639011347	[423]	[603,639,606,286]	6	hSpCas9	negative selection	8777925
77379893	77379916	11	-	27260156	PATU8988T	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.5766140310556602	[253]	[419,327,373,335]	8	hSpCas9	negative selection	8777926
77374327	77374350	11	-	27260156	PATU8988T	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.018362665936521194	[500]	[515,652,375,427]	0	hSpCas9	negative selection	8777927
77340752	77340775	11	-	27260156	T47D	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.40932101759298734	[1566]	[1571,1333,1276,1318]	-7	hSpCas9	negative selection	8834923
77379304	77379327	11	-	27260156	T47D	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.5808228298199465	[39]	[51,35,57,137]	9	hSpCas9	negative selection	8834924
77379240	77379263	11	-	27260156	T47D	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.2773048375425713	[104]	[154,125,157,155]	6	hSpCas9	negative selection	8834925
77340645	77340668	11	-	27260156	T47D	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.19540504668247005	[423]	[374,425,464,459]	-4	hSpCas9	negative selection	8899176
77379893	77379916	11	-	27260156	T47D	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.2489544470756513	[253]	[415,314,312,366]	5	hSpCas9	negative selection	8899177
77374327	77374350	11	-	27260156	T47D	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.16596979374100512	[500]	[637,637,686,656]	3	hSpCas9	negative selection	8899178
77340752	77340775	11	-	27260156	SU8686	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.3053455234381095	[1566]	[1911,1662,1852,2336]	5	hSpCas9	negative selection	8956174
77379304	77379327	11	-	27260156	SU8686	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.7165325967603364	[39]	[119,188,86,113]	9	hSpCas9	negative selection	8956175
77379240	77379263	11	-	27260156	SU8686	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.6321139230591939	[104]	[140,127,156,236]	9	hSpCas9	negative selection	8956176
77340645	77340668	11	-	27260156	SU8686	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.2255040577272754	[423]	[310,373,358,401]	-4	hSpCas9	negative selection	9020427
77379893	77379916	11	-	27260156	SU8686	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	1.077628840558548	[253]	[465,479,567,640]	9	hSpCas9	negative selection	9020428
77374327	77374350	11	-	27260156	SU8686	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	-0.18914582081975556	[500]	[365,478,445,453]	-3	hSpCas9	negative selection	9020429
77340752	77340775	11	-	27260156	SKES1	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.02650253238063538	[1566]	[1681,1895,1439,1406]	0	hSpCas9	negative selection	9077425
77379304	77379327	11	-	27260156	SKES1	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	1.4960347622566106	[39]	[105,60,200,76]	9	hSpCas9	negative selection	9077426
77379240	77379263	11	-	27260156	SKES1	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.34089997322216714	[104]	[118,155,150,106]	7	hSpCas9	negative selection	9077427
77340645	77340668	11	-	27260156	SKES1	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.2074351646013592	[423]	[408,496,336,253]	-4	hSpCas9	negative selection	9141678
77379893	77379916	11	-	27260156	SKES1	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.39031605811154346	[253]	[333,496,194,321]	7	hSpCas9	negative selection	9141679
77374327	77374350	11	-	27260156	SKES1	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.07162528477943537	[500]	[475,635,459,529]	1	hSpCas9	negative selection	9141680
77340752	77340775	11	-	27260156	TOV112D	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.06999010663612726	[1566]	[1528,1611,1968,915]	-1	hSpCas9	negative selection	9198676
77379304	77379327	11	-	27260156	TOV112D	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.9398140599133784	[39]	[34,80,83,103]	9	hSpCas9	negative selection	9198677
77379240	77379263	11	-	27260156	TOV112D	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.48134657590658936	[104]	[29,46,50,159]	-7	hSpCas9	negative selection	9198678
77340645	77340668	11	-	27260156	TOV112D	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.060951389040907605	[423]	[593,496,219,355]	-1	hSpCas9	negative selection	9262929
77379893	77379916	11	-	27260156	TOV112D	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.23312686110570757	[253]	[215,249,429,280]	4	hSpCas9	negative selection	9262930
77374327	77374350	11	-	27260156	TOV112D	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.3781614706873499	[500]	[546,576,688,763]	7	hSpCas9	negative selection	9262931
77340752	77340775	11	-	27260156	TC71	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	0.08844273961501381	[1566]	[1499,1851,1882,1919]	2	hSpCas9	negative selection	9319927
77379304	77379327	11	-	27260156	TC71	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.8018002800050401	[39]	[58,75,47,119]	9	hSpCas9	negative selection	9319928
77379240	77379263	11	-	27260156	TC71	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	-0.3250181460836692	[104]	[163,96,52,41]	-7	hSpCas9	negative selection	9319929
77340645	77340668	11	-	27260156	TC71	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	0.08129101995877819	[423]	[389,484,611,430]	2	hSpCas9	negative selection	9384180
77379893	77379916	11	-	27260156	TC71	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.16723923084271974	[253]	[256,324,285,359]	4	hSpCas9	negative selection	9384181
77374327	77374350	11	-	27260156	TC71	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.08944187223911208	[500]	[543,566,543,635]	2	hSpCas9	negative selection	9384182
77340752	77340775	11	-	27260156	TC32	viability	TATTCCTGCAGTTACCTCGTGGG	PAK1	ENSG00000149269	-0.3153079024270933	[1566]	[1102,955,1507,1250]	-5	hSpCas9	negative selection	9441178
77379304	77379327	11	-	27260156	TC32	viability	GAAAAACCCGCAGGCTGTTCTGG	PAK1	ENSG00000149269	0.5451487686838372	[39]	[51,20,81,80]	8	hSpCas9	negative selection	9441179
77379240	77379263	11	-	27260156	TC32	viability	CAGAAATACATGAGCTTTACAGG	PAK1	ENSG00000149269	0.538342575345117	[104]	[174,123,144,121]	8	hSpCas9	negative selection	9441180
77340645	77340668	11	-	27260156	TC32	viability	AATTGCAGCTGTGTGCCGTGAGG	PAK1	ENSG00000149269	-0.017181907754277775	[423]	[368,282,448,511]	0	hSpCas9	negative selection	9505431
77379893	77379916	11	-	27260156	TC32	viability	TGCTGTCACAGGGGAGTTTACGG	PAK1	ENSG00000149269	0.6376980298711175	[253]	[251,317,634,337]	9	hSpCas9	negative selection	9505432
77374327	77374350	11	-	27260156	TC32	viability	TTACAATTCTTCTAATGCCTTGG	PAK1	ENSG00000149269	0.023992811299232003	[500]	[441,235,703,652]	0	hSpCas9	negative selection	9505433
77336073	77336096	11	+	27869803	HPAFII	viability after 27 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.3760306337499747	[96]	[69,62,NaN]	3	hSpCas9	negative selection	9544182
77337387	77337410	11	-	27869803	HPAFII	viability after 27 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.27550375779601033	[179]	[149,84,NaN]	2	hSpCas9	negative selection	9544183
77349246	77349269	11	-	27869803	HPAFII	viability after 27 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.20003657559475563	[184]	[18,188,NaN]	1	hSpCas9	negative selection	9544184
77353536	77353559	11	-	27869803	HPAFII	viability after 27 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.7724828706110303	[428]	[441,337,NaN]	6	hSpCas9	negative selection	9544185
77355788	77355811	11	-	27869803	HPAFII	viability after 27 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.2850282310568646	[797]	[784,282,NaN]	2	hSpCas9	negative selection	9544186
77392457	77392480	11	+	27869803	HPAFII	viability after 27 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.36000003188278384	[224]	[141,159,NaN]	3	hSpCas9	negative selection	9544187
77336073	77336096	11	+	27869803	HPAFII	viability after 15 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.2671689006399538	[96]	[204,75,NaN]	3	hSpCas9	negative selection	9626631
77337387	77337410	11	-	27869803	HPAFII	viability after 15 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.12094058676817193	[179]	[218,204,NaN]	1	hSpCas9	negative selection	9626632
77349246	77349269	11	-	27869803	HPAFII	viability after 15 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.16678566703010778	[184]	[194,165,NaN]	-2	hSpCas9	negative selection	9626633
77353536	77353559	11	-	27869803	HPAFII	viability after 15 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.20272750711780085	[428]	[915,290,NaN]	2	hSpCas9	negative selection	9626634
77355788	77355811	11	-	27869803	HPAFII	viability after 15 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.203249201393626	[797]	[1281,410,NaN]	-2	hSpCas9	negative selection	9626635
77392457	77392480	11	+	27869803	HPAFII	viability after 15 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.560009941332014	[224]	[374,344,NaN]	6	hSpCas9	negative selection	9626636
77336073	77336096	11	+	27869803	ASPC1	viability after 36 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.3564690808845339	[369]	[408,135,15]	3	hSpCas9	negative selection	9709080
77337387	77337410	11	-	27869803	ASPC1	viability after 36 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.09093221672248711	[531]	[222,188,184]	0	hSpCas9	negative selection	9709081
77349246	77349269	11	-	27869803	ASPC1	viability after 36 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	-0.6986394515859036	[523]	[325,7,44]	-6	hSpCas9	negative selection	9709082
77353536	77353559	11	-	27869803	ASPC1	viability after 36 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	1.173079771314028	[836]	[1039,273,689]	9	hSpCas9	negative selection	9709083
77355788	77355811	11	-	27869803	ASPC1	viability after 36 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	-0.29300993586494917	[1900]	[705,394,536]	-3	hSpCas9	negative selection	9709084
77392457	77392480	11	+	27869803	ASPC1	viability after 36 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.0596972063312801	[709]	[602,184,20]	0	hSpCas9	negative selection	9709085
77336073	77336096	11	+	27869803	PATU8988S	viability after 35 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.1842028437417309	[237]	[68,148,NaN]	2	hSpCas9	negative selection	9791529
77337387	77337410	11	-	27869803	PATU8988S	viability after 35 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.19585039336174903	[500]	[281,179,NaN]	2	hSpCas9	negative selection	9791530
77349246	77349269	11	-	27869803	PATU8988S	viability after 35 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.21613119321065646	[431]	[248,154,NaN]	2	hSpCas9	negative selection	9791531
77353536	77353559	11	-	27869803	PATU8988S	viability after 35 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.49678789559175296	[783]	[468,421,NaN]	5	hSpCas9	negative selection	9791532
77355788	77355811	11	-	27869803	PATU8988S	viability after 35 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.8983019644629964	[1497]	[1347,899,NaN]	8	hSpCas9	negative selection	9791533
77392457	77392480	11	+	27869803	PATU8988S	viability after 35 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	-0.2123061142518584	[435]	[181,120,NaN]	-2	hSpCas9	negative selection	9791534
77336073	77336096	11	+	27869803	PATU8988S	viability after 31 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.2885865764088821	[237]	[96,139,NaN]	3	hSpCas9	negative selection	9873978
77337387	77337410	11	-	27869803	PATU8988S	viability after 31 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.08193524308650516	[500]	[232,183,NaN]	1	hSpCas9	negative selection	9873979
77349246	77349269	11	-	27869803	PATU8988S	viability after 31 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.4059754911267376	[431]	[218,239,NaN]	5	hSpCas9	negative selection	9873980
77353536	77353559	11	-	27869803	PATU8988S	viability after 31 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.5632797792783096	[783]	[497,415,NaN]	6	hSpCas9	negative selection	9873981
77355788	77355811	11	-	27869803	PATU8988S	viability after 31 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.8893103169463463	[1497]	[1134,1069,NaN]	8	hSpCas9	negative selection	9873982
77392457	77392480	11	+	27869803	PATU8988S	viability after 31 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.07807064860067511	[435]	[179,187,NaN]	1	hSpCas9	negative selection	9873983
77336073	77336096	11	+	27869803	HPAFII	viability after 35 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	0.6840062363398601	[96]	[100,53,NaN]	4	hSpCas9	negative selection	9956427
77337387	77337410	11	-	27869803	HPAFII	viability after 35 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	0.17529583664228204	[179]	[132,68,NaN]	1	hSpCas9	negative selection	9956428
77349246	77349269	11	-	27869803	HPAFII	viability after 35 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.2710878939418579	[184]	[25,210,NaN]	1	hSpCas9	negative selection	9956429
77353536	77353559	11	-	27869803	HPAFII	viability after 35 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.40216164217281736	[428]	[167,420,NaN]	2	hSpCas9	negative selection	9956430
77355788	77355811	11	-	27869803	HPAFII	viability after 35 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.783401824940464	[797]	[1009,340,NaN]	5	hSpCas9	negative selection	9956431
77392457	77392480	11	+	27869803	HPAFII	viability after 35 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.5098459618324733	[224]	[188,131,NaN]	3	hSpCas9	negative selection	9956432
77336073	77336096	11	+	27869803	HPAFII	viability after 31 days	TGGTAACACTCACTCTCAGAGGG	PAK1	ENSG00000149269	1.0021153096373059	[96]	[120,95,NaN]	7	hSpCas9	negative selection	10038876
77337387	77337410	11	-	27869803	HPAFII	viability after 31 days	GGAGTTCTTGCATTCGAACCAGG	PAK1	ENSG00000149269	-0.10685602217952778	[179]	[55,134,NaN]	0	hSpCas9	negative selection	10038877
77349246	77349269	11	-	27869803	HPAFII	viability after 31 days	ACAGCAATGGATGTGGCCACAGG	PAK1	ENSG00000149269	0.45695247204364914	[184]	[19,275,NaN]	3	hSpCas9	negative selection	10038878
77353536	77353559	11	-	27869803	HPAFII	viability after 31 days	CGGTTTGAGAAGATTGGACAAGG	PAK1	ENSG00000149269	0.4448635607572684	[428]	[287,370,NaN]	3	hSpCas9	negative selection	10038879
77355788	77355811	11	-	27869803	HPAFII	viability after 31 days	TGTCACTCCAACTCGGGACGTGG	PAK1	ENSG00000149269	0.560743823365319	[797]	[952,341,NaN]	4	hSpCas9	negative selection	10038880
77392457	77392480	11	+	27869803	HPAFII	viability after 31 days	TGCTGGTATTTCTCATCGGAGGG	PAK1	ENSG00000149269	0.8866480682376796	[224]	[187,282,NaN]	6	hSpCas9	negative selection	10038881
77392411	77392434	11	-	28162770	P31FUJ	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.6320723878349059	[181]	[137]	-5	hSpCas9	negative selection	10206422
77392440	77392463	11	+	28162770	P31FUJ	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	1.2237119308406246	[89]	[246]	9	hSpCas9	negative selection	10206423
77379297	77379320	11	+	28162770	P31FUJ	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.44257559152899595	[133]	[213]	5	hSpCas9	negative selection	10206424
77353535	77353558	11	-	28162770	P31FUJ	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.7523398368585081	[243]	[482]	8	hSpCas9	negative selection	10206425
77392458	77392481	11	+	28162770	P31FUJ	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.17036119457617332	[182]	[241]	1	hSpCas9	negative selection	10206426
77355781	77355804	11	+	28162770	P31FUJ	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.0949016289771425	[348]	[437]	1	hSpCas9	negative selection	10206427
77349238	77349261	11	-	28162770	P31FUJ	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.007379530365597464	[402]	[475]	0	hSpCas9	negative selection	10206428
77336226	77336249	11	-	28162770	P31FUJ	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.19309249461240735	[214]	[220]	-2	hSpCas9	negative selection	10206429
77332777	77332800	11	+	28162770	P31FUJ	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.12120586146690565	[168]	[215]	1	hSpCas9	negative selection	10206430
77332752	77332775	11	-	28162770	P31FUJ	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-0.8010859639889045	[85]	[57]	-6	hSpCas9	negative selection	10206431
77392411	77392434	11	-	28162770	TF1	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	NaN	[NaN]	[123]	1	hSpCas9	negative selection	10375788
77392440	77392463	11	+	28162770	TF1	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	NaN	[NaN]	[55]	1	hSpCas9	negative selection	10375789
77379297	77379320	11	+	28162770	TF1	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	NaN	[NaN]	[129]	1	hSpCas9	negative selection	10375790
77353535	77353558	11	-	28162770	TF1	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	NaN	[NaN]	[407]	1	hSpCas9	negative selection	10375791
77392458	77392481	11	+	28162770	TF1	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	NaN	[NaN]	[56]	1	hSpCas9	negative selection	10375792
77355781	77355804	11	+	28162770	TF1	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	NaN	[NaN]	[377]	1	hSpCas9	negative selection	10375793
77349238	77349261	11	-	28162770	TF1	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	NaN	[NaN]	[228]	1	hSpCas9	negative selection	10375794
77336226	77336249	11	-	28162770	TF1	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	NaN	[NaN]	[231]	1	hSpCas9	negative selection	10375795
77332777	77332800	11	+	28162770	TF1	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	NaN	[NaN]	[117]	1	hSpCas9	negative selection	10375796
77332752	77332775	11	-	28162770	TF1	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	NaN	[NaN]	[20]	1	hSpCas9	negative selection	10375797
77392411	77392434	11	-	28162770	SKM1	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-1.0494121941250778	[47]	[40]	-6	hSpCas9	negative selection	10545154
77392440	77392463	11	+	28162770	SKM1	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-0.3563381256731937	[41]	[57]	-3	hSpCas9	negative selection	10545155
77379297	77379320	11	+	28162770	SKM1	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.5930358012568385	[47]	[127]	6	hSpCas9	negative selection	10545156
77353535	77353558	11	-	28162770	SKM1	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.3369018973290548	[72]	[162]	3	hSpCas9	negative selection	10545157
77392458	77392481	11	+	28162770	SKM1	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.2500953501262956	[73]	[109]	-2	hSpCas9	negative selection	10545158
77355781	77355804	11	+	28162770	SKM1	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.13910928858439942	[93]	[182]	1	hSpCas9	negative selection	10545159
77349238	77349261	11	-	28162770	SKM1	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.14825095858394255	[97]	[191]	1	hSpCas9	negative selection	10545160
77336226	77336249	11	-	28162770	SKM1	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.3955897370506213	[42]	[99]	4	hSpCas9	negative selection	10545161
77332777	77332800	11	+	28162770	SKM1	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.7618807055844345	[46]	[48]	-5	hSpCas9	negative selection	10545162
77332752	77332775	11	-	28162770	SKM1	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.532495080827021	[17]	[10]	-7	hSpCas9	negative selection	10545163
77392411	77392434	11	-	28162770	SKM1	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-1.0494121941250778	[47]	[40]	-6	hSpCas9	negative selection	10714520
77392440	77392463	11	+	28162770	SKM1	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-0.3563381256731937	[41]	[57]	-3	hSpCas9	negative selection	10714521
77379297	77379320	11	+	28162770	SKM1	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.5930358012568385	[47]	[127]	6	hSpCas9	negative selection	10714522
77353535	77353558	11	-	28162770	SKM1	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.3369018973290548	[72]	[162]	3	hSpCas9	negative selection	10714523
77392458	77392481	11	+	28162770	SKM1	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.2500953501262956	[73]	[109]	-2	hSpCas9	negative selection	10714524
77355781	77355804	11	+	28162770	SKM1	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.13910928858439942	[93]	[182]	1	hSpCas9	negative selection	10714525
77349238	77349261	11	-	28162770	SKM1	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.14825095858394255	[97]	[191]	1	hSpCas9	negative selection	10714526
77336226	77336249	11	-	28162770	SKM1	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.3955897370506213	[42]	[99]	4	hSpCas9	negative selection	10714527
77332777	77332800	11	+	28162770	SKM1	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.7618807055844345	[46]	[48]	-5	hSpCas9	negative selection	10714528
77332752	77332775	11	-	28162770	SKM1	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.532495080827021	[17]	[10]	-7	hSpCas9	negative selection	10714529
77392411	77392434	11	-	28162770	PL21	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	1.1506709382250202	[177]	[171]	8	hSpCas9	negative selection	10883886
77392440	77392463	11	+	28162770	PL21	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.20013961448932016	[83]	[41]	1	hSpCas9	negative selection	10883887
77379297	77379320	11	+	28162770	PL21	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	1.1229717539668602	[191]	[181]	8	hSpCas9	negative selection	10883888
77353535	77353558	11	-	28162770	PL21	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.709714993495737	[346]	[246]	6	hSpCas9	negative selection	10883889
77392458	77392481	11	+	28162770	PL21	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.42435125041847366	[221]	[71]	-3	hSpCas9	negative selection	10883890
77355781	77355804	11	+	28162770	PL21	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.021083601294879295	[316]	[139]	0	hSpCas9	negative selection	10883891
77349238	77349261	11	-	28162770	PL21	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	1.2963549297486234	[347]	[371]	9	hSpCas9	negative selection	10883892
77336226	77336249	11	-	28162770	PL21	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.43393679909801747	[215]	[126]	4	hSpCas9	negative selection	10883893
77332777	77332800	11	+	28162770	PL21	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.09120524293615584	[109]	[50]	0	hSpCas9	negative selection	10883894
77332752	77332775	11	-	28162770	PL21	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-0.3303751022094594	[77]	[26]	-2	hSpCas9	negative selection	10883895
77392411	77392434	11	-	28162770	P31FUJ	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.6320723878349059	[181]	[137]	-5	hSpCas9	negative selection	11053252
77392440	77392463	11	+	28162770	P31FUJ	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	1.2237119308406246	[89]	[246]	9	hSpCas9	negative selection	11053253
77379297	77379320	11	+	28162770	P31FUJ	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.44257559152899595	[133]	[213]	5	hSpCas9	negative selection	11053254
77353535	77353558	11	-	28162770	P31FUJ	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.7523398368585081	[243]	[482]	8	hSpCas9	negative selection	11053255
77392458	77392481	11	+	28162770	P31FUJ	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.17036119457617332	[182]	[241]	1	hSpCas9	negative selection	11053256
77355781	77355804	11	+	28162770	P31FUJ	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.0949016289771425	[348]	[437]	1	hSpCas9	negative selection	11053257
77349238	77349261	11	-	28162770	P31FUJ	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.007379530365597464	[402]	[475]	0	hSpCas9	negative selection	11053258
77336226	77336249	11	-	28162770	P31FUJ	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.19309249461240735	[214]	[220]	-2	hSpCas9	negative selection	11053259
77332777	77332800	11	+	28162770	P31FUJ	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.12120586146690565	[168]	[215]	1	hSpCas9	negative selection	11053260
77332752	77332775	11	-	28162770	P31FUJ	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-0.8010859639889045	[85]	[57]	-6	hSpCas9	negative selection	11053261
77392411	77392434	11	-	28162770	OCIAML5	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.001384765783061126	[87]	[158]	0	hSpCas9	negative selection	11222618
77392440	77392463	11	+	28162770	OCIAML5	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.748577552089429	[60]	[184]	6	hSpCas9	negative selection	11222619
77379297	77379320	11	+	28162770	OCIAML5	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.22322155644024017	[83]	[176]	1	hSpCas9	negative selection	11222620
77353535	77353558	11	-	28162770	OCIAML5	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.7416661394848914	[163]	[494]	6	hSpCas9	negative selection	11222621
77392458	77392481	11	+	28162770	OCIAML5	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.6218646174684153	[107]	[299]	5	hSpCas9	negative selection	11222622
77355781	77355804	11	+	28162770	OCIAML5	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.974147378444923	[151]	[538]	8	hSpCas9	negative selection	11222623
77349238	77349261	11	-	28162770	OCIAML5	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	-0.4181700443338373	[227]	[307]	-3	hSpCas9	negative selection	11222624
77336226	77336249	11	-	28162770	OCIAML5	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.06932359443963682	[131]	[249]	0	hSpCas9	negative selection	11222625
77332777	77332800	11	+	28162770	OCIAML5	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	1.1562969020706297	[85]	[345]	8	hSpCas9	negative selection	11222626
77332752	77332775	11	-	28162770	OCIAML5	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-4.244383993642757	[41]	[3]	-9	hSpCas9	negative selection	11222627
77392411	77392434	11	-	28162770	EOL1	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.3583828663035044	[304]	[205]	3	hSpCas9	negative selection	11391984
77392440	77392463	11	+	28162770	EOL1	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.5493362527848792	[130]	[100]	5	hSpCas9	negative selection	11391985
77379297	77379320	11	+	28162770	EOL1	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.1356300977696067	[310]	[179]	1	hSpCas9	negative selection	11391986
77353535	77353558	11	-	28162770	EOL1	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.966649604450364	[438]	[451]	8	hSpCas9	negative selection	11391987
77392458	77392481	11	+	28162770	EOL1	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.2700443375963014	[339]	[215]	2	hSpCas9	negative selection	11391988
77355781	77355804	11	+	28162770	EOL1	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.2555958022390167	[672]	[296]	-2	hSpCas9	negative selection	11391989
77349238	77349261	11	-	28162770	EOL1	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.4832135285834635	[405]	[298]	4	hSpCas9	negative selection	11391990
77336226	77336249	11	-	28162770	EOL1	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.10512001721235559	[239]	[135]	0	hSpCas9	negative selection	11391991
77332777	77332800	11	+	28162770	EOL1	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.07545222842946542	[167]	[83]	0	hSpCas9	negative selection	11391992
77332752	77332775	11	-	28162770	EOL1	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.6115051286696753	[115]	[19]	-7	hSpCas9	negative selection	11391993
77392411	77392434	11	-	28162770	MOLM13	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.016379119557104388	[248]	[177]	0	hSpCas9	negative selection	11561350
77392440	77392463	11	+	28162770	MOLM13	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-0.5083945494816136	[180]	[91]	-3	hSpCas9	negative selection	11561351
77379297	77379320	11	+	28162770	MOLM13	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	1.0217413497257046	[249]	[366]	7	hSpCas9	negative selection	11561352
77353535	77353558	11	-	28162770	MOLM13	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.6913776594022998	[375]	[438]	5	hSpCas9	negative selection	11561353
77392458	77392481	11	+	28162770	MOLM13	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.30597081923444613	[291]	[260]	2	hSpCas9	negative selection	11561354
77355781	77355804	11	+	28162770	MOLM13	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	1.2397029964331567	[498]	[851]	8	hSpCas9	negative selection	11561355
77349238	77349261	11	-	28162770	MOLM13	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.47129598298782827	[422]	[423]	3	hSpCas9	negative selection	11561356
77336226	77336249	11	-	28162770	MOLM13	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-1.2497108877546963	[295]	[89]	-6	hSpCas9	negative selection	11561357
77332777	77332800	11	+	28162770	MOLM13	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	0.4213467956075486	[251]	[243]	3	hSpCas9	negative selection	11561358
77332752	77332775	11	-	28162770	MOLM13	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-2.0380015481853784	[141]	[24]	-7	hSpCas9	negative selection	11561359
77392411	77392434	11	-	28162770	HEL	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.5323478745577344	[209]	[92]	-4	hSpCas9	negative selection	11730716
77392440	77392463	11	+	28162770	HEL	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.008023296082101172	[117]	[75]	0	hSpCas9	negative selection	11730717
77379297	77379320	11	+	28162770	HEL	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.3647540847005916	[193]	[159]	3	hSpCas9	negative selection	11730718
77353535	77353558	11	-	28162770	HEL	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.507254475887074	[356]	[324]	4	hSpCas9	negative selection	11730719
77392458	77392481	11	+	28162770	HEL	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.11380713113536539	[271]	[160]	-1	hSpCas9	negative selection	11730720
77355781	77355804	11	+	28162770	HEL	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	-0.02638411638479765	[449]	[282]	0	hSpCas9	negative selection	11730721
77349238	77349261	11	-	28162770	HEL	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.287822325159084	[375]	[293]	2	hSpCas9	negative selection	11730722
77336226	77336249	11	-	28162770	HEL	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.5964451797264217	[189]	[183]	5	hSpCas9	negative selection	11730723
77332777	77332800	11	+	28162770	HEL	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.8838069824954771	[120]	[41]	-5	hSpCas9	negative selection	11730724
77332752	77332775	11	-	28162770	HEL	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.679189262887005	[89]	[17]	-7	hSpCas9	negative selection	11730725
77392411	77392434	11	-	28162770	MV411	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.552292132311196	[152]	[188]	5	hSpCas9	negative selection	11900082
77392440	77392463	11	+	28162770	MV411	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-2.161953385354926	[84]	[15]	-8	hSpCas9	negative selection	11900083
77379297	77379320	11	+	28162770	MV411	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.4210856382678051	[171]	[193]	4	hSpCas9	negative selection	11900084
77353535	77353558	11	-	28162770	MV411	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.2020270910928288	[354]	[343]	1	hSpCas9	negative selection	11900085
77392458	77392481	11	+	28162770	MV411	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.25613662309772606	[223]	[157]	-2	hSpCas9	negative selection	11900086
77355781	77355804	11	+	28162770	MV411	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.18621075153232924	[360]	[345]	1	hSpCas9	negative selection	11900087
77349238	77349261	11	-	28162770	MV411	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	-0.2746372439829905	[302]	[210]	-2	hSpCas9	negative selection	11900088
77336226	77336249	11	-	28162770	MV411	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.9393152554204435	[155]	[251]	8	hSpCas9	negative selection	11900089
77332777	77332800	11	+	28162770	MV411	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.062106819648754474	[144]	[116]	0	hSpCas9	negative selection	11900090
77332752	77332775	11	-	28162770	MV411	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.3848306647167377	[61]	[19]	-7	hSpCas9	negative selection	11900091
77392411	77392434	11	-	28162770	MONOMAC1	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.960109549661456	[125]	[280]	8	hSpCas9	negative selection	12069448
77392440	77392463	11	+	28162770	MONOMAC1	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-1.7459300930731882	[78]	[26]	-8	hSpCas9	negative selection	12069449
77379297	77379320	11	+	28162770	MONOMAC1	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	-0.9039898721593203	[110]	[67]	-6	hSpCas9	negative selection	12069450
77353535	77353558	11	-	28162770	MONOMAC1	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.7469291180261185	[209]	[403]	7	hSpCas9	negative selection	12069451
77392458	77392481	11	+	28162770	MONOMAC1	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.3079573489581874	[135]	[192]	3	hSpCas9	negative selection	12069452
77355781	77355804	11	+	28162770	MONOMAC1	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.5412049037939229	[225]	[376]	5	hSpCas9	negative selection	12069453
77349238	77349261	11	-	28162770	MONOMAC1	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.35699379410971155	[219]	[322]	3	hSpCas9	negative selection	12069454
77336226	77336249	11	-	28162770	MONOMAC1	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	-0.02300744728450467	[116]	[131]	0	hSpCas9	negative selection	12069455
77332777	77332800	11	+	28162770	MONOMAC1	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	1.1453553503875242	[83]	[212]	8	hSpCas9	negative selection	12069456
77332752	77332775	11	-	28162770	MONOMAC1	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	1.4309943755534884	[43]	[135]	9	hSpCas9	negative selection	12069457
77392411	77392434	11	-	28162770	OCIAML2	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	-0.13434643737772867	[39]	[201]	-1	hSpCas9	negative selection	12238814
77392440	77392463	11	+	28162770	OCIAML2	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	0.7924045805916329	[14]	[143]	7	hSpCas9	negative selection	12238815
77379297	77379320	11	+	28162770	OCIAML2	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	0.5293701747578388	[31]	[255]	5	hSpCas9	negative selection	12238816
77353535	77353558	11	-	28162770	OCIAML2	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.2812861103900166	[71]	[484]	2	hSpCas9	negative selection	12238817
77392458	77392481	11	+	28162770	OCIAML2	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	0.16617160699861294	[40]	[254]	1	hSpCas9	negative selection	12238818
77355781	77355804	11	+	28162770	OCIAML2	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.5862013973545147	[55]	[465]	6	hSpCas9	negative selection	12238819
77349238	77349261	11	-	28162770	OCIAML2	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.4627329817275485	[35]	[274]	4	hSpCas9	negative selection	12238820
77336226	77336249	11	-	28162770	OCIAML2	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.9037656895393367	[26]	[279]	8	hSpCas9	negative selection	12238821
77332777	77332800	11	+	28162770	OCIAML2	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.08980804130123021	[23]	[124]	0	hSpCas9	negative selection	12238822
77332752	77332775	11	-	28162770	OCIAML2	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-2.631094497435407	[18]	[16]	-9	hSpCas9	negative selection	12238823
77392411	77392434	11	-	28162770	OCIAML3	viability	GATGCTGGAACCCTAAACCATGG	PAK1	ENSG00000149269	0.024313457343423994	[56]	[174]	0	hSpCas9	negative selection	12408180
77392440	77392463	11	+	28162770	OCIAML3	viability	GCCGGCTCCAATCATAGTGCTGG	PAK1	ENSG00000149269	-0.1258588045857567	[29]	[82]	-1	hSpCas9	negative selection	12408181
77379297	77379320	11	+	28162770	OCIAML3	viability	AACACATCCAGAACAGCCTGCGG	PAK1	ENSG00000149269	-0.22901082354491475	[32]	[84]	-2	hSpCas9	negative selection	12408182
77353535	77353558	11	-	28162770	OCIAML3	viability	GGTTTGAGAAGATTGGACAAGGG	PAK1	ENSG00000149269	0.15073530443376243	[73]	[247]	1	hSpCas9	negative selection	12408183
77392458	77392481	11	+	28162770	OCIAML3	viability	GCTGGTATTTCTCATCGGAGGGG	PAK1	ENSG00000149269	-0.9049367362622683	[48]	[78]	-6	hSpCas9	negative selection	12408184
77355781	77355804	11	+	28162770	OCIAML3	viability	GATGTAGCCACGTCCCGAGTTGG	PAK1	ENSG00000149269	0.5953348149453896	[81]	[373]	5	hSpCas9	negative selection	12408185
77349238	77349261	11	-	28162770	OCIAML3	viability	GGATGTGGCCACAGGACAGGAGG	PAK1	ENSG00000149269	0.7055526415347447	[64]	[319]	6	hSpCas9	negative selection	12408186
77336226	77336249	11	-	28162770	OCIAML3	viability	GCAGAGCAAACGGAGCACCATGG	PAK1	ENSG00000149269	0.37122694151516	[41]	[163]	3	hSpCas9	negative selection	12408187
77332777	77332800	11	+	28162770	OCIAML3	viability	GACAGCGGTTCAGAAAGTCCCGG	PAK1	ENSG00000149269	-0.4636110030684881	[36]	[80]	-4	hSpCas9	negative selection	12408188
77332752	77332775	11	-	28162770	OCIAML3	viability	GAGATGGATGTGGAGAAGAGAGG	PAK1	ENSG00000149269	-1.1665864165894868	[28]	[38]	-7	hSpCas9	negative selection	12408189