Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:5562
• Symbol:PRKAA1
• Description:protein kinase AMP-activated catalytic subunit alpha 1

DataSource: http://genomecrispr.dkfz.de/#!/results/PRKAA1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
40777509	40777532	5	-	26472758	Jiyoye	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.8853362254047541	[266]	[108]	-6	hSpCas9	negative selection	127253
40764858	40764881	5	-	26472758	Jiyoye	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-1.2332822318069865	[105]	[33]	-7	hSpCas9	negative selection	127254
40767652	40767675	5	-	26472758	Jiyoye	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.155419306186828	[191]	[64]	-7	hSpCas9	negative selection	127255
40798131	40798154	5	-	26472758	Jiyoye	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.5928246184941264	[11]	[5]	-4	hSpCas9	negative selection	127256
40798088	40798111	5	-	26472758	Jiyoye	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-0.32052729133136476	[206]	[124]	-2	hSpCas9	negative selection	127257
40767485	40767508	5	-	26472758	Jiyoye	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.9454244091239066	[308]	[120]	-6	hSpCas9	negative selection	127258
40777458	40777481	5	+	26472758	Jiyoye	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.30204522217742846	[102]	[62]	-2	hSpCas9	negative selection	127259
40771788	40771811	5	-	26472758	Jiyoye	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-1.0299805237576904	[240]	[88]	-6	hSpCas9	negative selection	127260
40767671	40767694	5	-	26472758	Jiyoye	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.7515223645131849	[287]	[128]	-5	hSpCas9	negative selection	127261
40764912	40764935	5	+	26472758	Jiyoye	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.028039999710600727	[95]	[70]	0	hSpCas9	negative selection	127262
40777509	40777532	5	-	26472758	KBM7	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.9634510161905024	[780,603]	[172,158]	-6	hSpCas9	negative selection	318371
40764858	40764881	5	-	26472758	KBM7	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.14799570242312643	[267,265]	[107,118]	-1	hSpCas9	negative selection	318372
40767652	40767675	5	-	26472758	KBM7	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-0.9912411244945436	[389,283]	[53,100]	-6	hSpCas9	negative selection	318373
40798131	40798154	5	-	26472758	KBM7	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-2.232091562280732	[115,130]	[21,3]	-8	hSpCas9	negative selection	318374
40798088	40798111	5	-	26472758	KBM7	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.1502693551057956	[1479,1058]	[318,217]	-7	hSpCas9	negative selection	318375
40767485	40767508	5	-	26472758	KBM7	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.5103065724695668	[756,604]	[117,105]	-8	hSpCas9	negative selection	318376
40777458	40777481	5	+	26472758	KBM7	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.9004538928813018	[515,482]	[191,66]	-6	hSpCas9	negative selection	318377
40771788	40771811	5	-	26472758	KBM7	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.3469402161943036	[937,1004]	[377,347]	-3	hSpCas9	negative selection	318378
40767671	40767694	5	-	26472758	KBM7	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.09207336123650578	[812,575]	[377,312]	0	hSpCas9	negative selection	318379
40764912	40764935	5	+	26472758	KBM7	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.06280045799650158	[311,329]	[151,164]	0	hSpCas9	negative selection	318380
40777509	40777532	5	-	26472758	Raji	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	0.28986158715899646	[355]	[100]	2	hSpCas9	negative selection	509489
40764858	40764881	5	-	26472758	Raji	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	0.5493880822527126	[158]	[53]	5	hSpCas9	negative selection	509490
40767652	40767675	5	-	26472758	Raji	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	0.449664692217129	[182]	[57]	4	hSpCas9	negative selection	509491
40798131	40798154	5	-	26472758	Raji	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.7752595139882419	[58]	[3]	-8	hSpCas9	negative selection	509492
40798088	40798111	5	-	26472758	Raji	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	0.38843199507874515	[450]	[136]	3	hSpCas9	negative selection	509493
40767485	40767508	5	-	26472758	Raji	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.08901367742990401	[329]	[71]	0	hSpCas9	negative selection	509494
40777458	40777481	5	+	26472758	Raji	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	0.24426534179398635	[210]	[57]	2	hSpCas9	negative selection	509495
40771788	40771811	5	-	26472758	Raji	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.18178846624737266	[478]	[97]	-1	hSpCas9	negative selection	509496
40767671	40767694	5	-	26472758	Raji	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.16812022242241098	[580]	[119]	-1	hSpCas9	negative selection	509497
40764912	40764935	5	+	26472758	Raji	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-1.5868776078383684	[232]	[17]	-8	hSpCas9	negative selection	509498
40777509	40777532	5	-	26472758	K562	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.4250183412182455	[632]	[397]	-3	hSpCas9	negative selection	700607
40764858	40764881	5	-	26472758	K562	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	0.3975755157278673	[249]	[277]	3	hSpCas9	negative selection	700608
40767652	40767675	5	-	26472758	K562	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	0.7042538409339478	[324]	[446]	6	hSpCas9	negative selection	700609
40798131	40798154	5	-	26472758	K562	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	0.06281192117649903	[92]	[81]	0	hSpCas9	negative selection	700610
40798088	40798111	5	-	26472758	K562	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	0.8638303874269628	[868]	[1334]	7	hSpCas9	negative selection	700611
40767485	40767508	5	-	26472758	K562	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	0.24186302276269034	[564]	[563]	1	hSpCas9	negative selection	700612
40777458	40777481	5	+	26472758	K562	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	1.4284506753041346	[293]	[667]	9	hSpCas9	negative selection	700613
40771788	40771811	5	-	26472758	K562	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	0.5266826049802934	[707]	[860]	4	hSpCas9	negative selection	700614
40767671	40767694	5	-	26472758	K562	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.17836432399608682	[647]	[618]	1	hSpCas9	negative selection	700615
40764912	40764935	5	+	26472758	K562	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.3342299991225069	[341]	[228]	-2	hSpCas9	negative selection	700616
40762842	40762865	5	+	26627737	DLD1	viability	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.703752878747115	[166]	[9,24,56]	-6	hSpCas9	negative selection	828811
40762943	40762966	5	-	26627737	DLD1	viability	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.17466941947196107	[331]	[39,191,121]	1	hSpCas9	negative selection	828812
40764907	40764930	5	-	26627737	DLD1	viability	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.06591956947544553	[183]	[58,74,48]	0	hSpCas9	negative selection	828813
40798090	40798113	5	-	26627737	DLD1	viability	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.3397654739313225	[1559]	[634,693,537]	3	hSpCas9	negative selection	828814
40762842	40762865	5	+	26627737	GBM cells	viability after 5 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.16480793485872036	[83]	[69,30]	2	hSpCas9	negative selection	911126
40762943	40762966	5	-	26627737	GBM cells	viability after 5 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.21577098022562582	[227]	[151,131]	3	hSpCas9	negative selection	911127
40764907	40764930	5	-	26627737	GBM cells	viability after 5 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.4835557619367278	[145]	[30,80]	-6	hSpCas9	negative selection	911128
40798090	40798113	5	-	26627737	GBM cells	viability after 5 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.1900956098780242	[625]	[406,358]	3	hSpCas9	negative selection	911129
40762842	40762865	5	+	26627737	GBM cells	viability after 13 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	1.093739249349759	[83]	[88,103]	9	hSpCas9	negative selection	993441
40762943	40762966	5	-	26627737	GBM cells	viability after 13 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.38019587454410786	[227]	[176,147]	5	hSpCas9	negative selection	993442
40764907	40764930	5	-	26627737	GBM cells	viability after 13 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.41826220870082975	[145]	[66,52]	-5	hSpCas9	negative selection	993443
40798090	40798113	5	-	26627737	GBM cells	viability after 13 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.3418797784429597	[625]	[419,438]	4	hSpCas9	negative selection	993444
40762842	40762865	5	+	26627737	GBM cells	viability after 21 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.6261023331633203	[83]	[71,68]	6	hSpCas9	negative selection	1075756
40762943	40762966	5	-	26627737	GBM cells	viability after 21 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.4059700688562913	[227]	[172,155]	4	hSpCas9	negative selection	1075757
40764907	40764930	5	-	26627737	GBM cells	viability after 21 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.8209477194105742	[145]	[58,31]	-6	hSpCas9	negative selection	1075758
40798090	40798113	5	-	26627737	GBM cells	viability after 21 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.8726079216729499	[625]	[664,583]	8	hSpCas9	negative selection	1075759
40762842	40762865	5	+	26627737	RPE1	viability after 9 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.05417267387708624	[254]	[57,114]	0	hSpCas9	negative selection	1158071
40762943	40762966	5	-	26627737	RPE1	viability after 9 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.4940206069164042	[399]	[140,221]	3	hSpCas9	negative selection	1158072
40764907	40764930	5	-	26627737	RPE1	viability after 9 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	1.0592882710988667	[292]	[112,291]	6	hSpCas9	negative selection	1158073
40798090	40798113	5	-	26627737	RPE1	viability after 9 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.9189395200472603	[1376]	[794,840]	6	hSpCas9	negative selection	1158074
40762842	40762865	5	+	26627737	RPE1	viability after 12 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.9607547098334569	[254]	[110,156]	6	hSpCas9	negative selection	1240386
40762943	40762966	5	-	26627737	RPE1	viability after 12 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.10916979215971945	[399]	[129,100]	0	hSpCas9	negative selection	1240387
40764907	40764930	5	-	26627737	RPE1	viability after 12 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.5324355491837577	[292]	[91,136]	3	hSpCas9	negative selection	1240388
40798090	40798113	5	-	26627737	RPE1	viability after 12 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.8204950362062604	[1376]	[800,496]	5	hSpCas9	negative selection	1240389
40762842	40762865	5	+	26627737	RPE1	viability after 15 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	1.3930532605371355	[254]	[107,267]	7	hSpCas9	negative selection	1322701
40762943	40762966	5	-	26627737	RPE1	viability after 15 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.2351125944240673	[399]	[94,169]	1	hSpCas9	negative selection	1322702
40764907	40764930	5	-	26627737	RPE1	viability after 15 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-1.01928233257699	[292]	[46,34]	-6	hSpCas9	negative selection	1322703
40798090	40798113	5	-	26627737	RPE1	viability after 15 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.9252174923070235	[1376]	[756,722]	5	hSpCas9	negative selection	1322704
40762842	40762865	5	+	26627737	RPE1	viability after 18 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	1.5743279015960905	[254]	[100,315]	8	hSpCas9	negative selection	1405016
40762943	40762966	5	-	26627737	RPE1	viability after 18 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.5379483066973099	[399]	[177,133]	3	hSpCas9	negative selection	1405017
40764907	40764930	5	-	26627737	RPE1	viability after 18 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.7067215789024679	[292]	[115,142]	4	hSpCas9	negative selection	1405018
40798090	40798113	5	-	26627737	RPE1	viability after 18 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	1.1697390840243411	[1376]	[952,710]	6	hSpCas9	negative selection	1405019
40762842	40762865	5	+	26627737	HeLa	viability after 8 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.3652257873986289	[105]	[123,31,37]	3	hSpCas9	negative selection	1487331
40762943	40762966	5	-	26627737	HeLa	viability after 8 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.223438663618218	[342]	[72,252,50]	-2	hSpCas9	negative selection	1487332
40764907	40764930	5	-	26627737	HeLa	viability after 8 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.7717758453047325	[285]	[33,71,101]	-6	hSpCas9	negative selection	1487333
40798090	40798113	5	-	26627737	HeLa	viability after 8 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.5017830750716894	[1019]	[337,208,392]	-5	hSpCas9	negative selection	1487334
40762842	40762865	5	+	26627737	HeLa	viability after 12 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	1.381493806028221	[105]	[228,54,67]	8	hSpCas9	negative selection	1569646
40762943	40762966	5	-	26627737	HeLa	viability after 12 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.542763021020584	[342]	[130,67,96]	-5	hSpCas9	negative selection	1569647
40764907	40764930	5	-	26627737	HeLa	viability after 12 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.5841039670472115	[285]	[137,33,70]	-5	hSpCas9	negative selection	1569648
40798090	40798113	5	-	26627737	HeLa	viability after 12 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.5014382966906075	[1019]	[518,529,745]	4	hSpCas9	negative selection	1569649
40762842	40762865	5	+	26627737	HeLa	viability after 15 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.6877743742885714	[105]	[83,58,64]	5	hSpCas9	negative selection	1651961
40762943	40762966	5	-	26627737	HeLa	viability after 15 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.06213256467209885	[342]	[42,266,88]	0	hSpCas9	negative selection	1651962
40764907	40764930	5	-	26627737	HeLa	viability after 15 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.7894418721855516	[285]	[84,71,42]	-6	hSpCas9	negative selection	1651963
40798090	40798113	5	-	26627737	HeLa	viability after 15 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.08073496777194311	[1019]	[493,331,494]	0	hSpCas9	negative selection	1651964
40762842	40762865	5	+	26627737	HeLa	viability after 18 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.7000556279877241	[105]	[54,51,64]	5	hSpCas9	negative selection	1734276
40762943	40762966	5	-	26627737	HeLa	viability after 18 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.04733538411063867	[342]	[29,222,88]	0	hSpCas9	negative selection	1734277
40764907	40764930	5	-	26627737	HeLa	viability after 18 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.7908356190977972	[285]	[58,56,42]	-6	hSpCas9	negative selection	1734278
40798090	40798113	5	-	26627737	HeLa	viability after 18 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.02918800286139933	[1019]	[306,267,494]	0	hSpCas9	negative selection	1734279
40762842	40762865	5	+	26627737	HCT116	viability after 6 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.18819883598056636	[464]	[165,7]	-1	hSpCas9	negative selection	1816591
40762943	40762966	5	-	26627737	HCT116	viability after 6 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.49494686305399993	[567]	[159,106]	2	hSpCas9	negative selection	1816592
40764907	40764930	5	-	26627737	HCT116	viability after 6 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-1.1938579363920456	[401]	[6,38]	-6	hSpCas9	negative selection	1816593
40798090	40798113	5	-	26627737	HCT116	viability after 6 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	1.1969463078950642	[1415]	[655,429]	6	hSpCas9	negative selection	1816594
40762842	40762865	5	+	26627737	HCT116	viability after 8 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.6189072160209208	[147]	[81,38,38]	-6	hSpCas9	negative selection	1898906
40762943	40762966	5	-	26627737	HCT116	viability after 8 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.535911255241132	[98]	[66,85,83]	5	hSpCas9	negative selection	1898907
40764907	40764930	5	-	26627737	HCT116	viability after 8 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.05907191994707306	[153]	[37,111,113]	0	hSpCas9	negative selection	1898908
40798090	40798113	5	-	26627737	HCT116	viability after 8 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.35845967577189697	[946]	[445,388,386]	-4	hSpCas9	negative selection	1898909
40762842	40762865	5	+	26627737	HCT116	viability after 9 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.6807275660087293	[464]	[53,95]	-5	hSpCas9	negative selection	1981221
40762943	40762966	5	-	26627737	HCT116	viability after 9 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	1.163256791514033	[567]	[337,340]	7	hSpCas9	negative selection	1981222
40764907	40764930	5	-	26627737	HCT116	viability after 9 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-1.3837626019508642	[401]	[77,11]	-7	hSpCas9	negative selection	1981223
40798090	40798113	5	-	26627737	HCT116	viability after 9 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.7557336768056817	[1415]	[643,633]	5	hSpCas9	negative selection	1981224
40762842	40762865	5	+	26627737	HCT116	viability after 12 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.3713969905470669	[147]	[37,46,96]	-4	hSpCas9	negative selection	2063536
40762943	40762966	5	-	26627737	HCT116	viability after 12 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.9761372272036446	[98]	[169,56,72]	8	hSpCas9	negative selection	2063537
40764907	40764930	5	-	26627737	HCT116	viability after 12 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.010337034003127465	[153]	[32,130,85]	0	hSpCas9	negative selection	2063538
40798090	40798113	5	-	26627737	HCT116	viability after 12 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.49270719259347406	[946]	[260,385,422]	-5	hSpCas9	negative selection	2063539
40762842	40762865	5	+	26627737	HCT116	viability after 15 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.01077625243225766	[147]	[86,47,117]	0	hSpCas9	negative selection	2145851
40762943	40762966	5	-	26627737	HCT116	viability after 15 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.6748884909310711	[98]	[88,84,97]	6	hSpCas9	negative selection	2145852
40764907	40764930	5	-	26627737	HCT116	viability after 15 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.22062185831673498	[153]	[89,111,27]	-2	hSpCas9	negative selection	2145853
40798090	40798113	5	-	26627737	HCT116	viability after 15 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.14427717300364606	[946]	[331,564,558]	-1	hSpCas9	negative selection	2145854
40762842	40762865	5	+	26627737	HCT116	viability after 18 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	-0.37299110859530593	[147]	[25,47,93]	-3	hSpCas9	negative selection	2228166
40762943	40762966	5	-	26627737	HCT116	viability after 18 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.13765449464237034	[98]	[27,70,57]	1	hSpCas9	negative selection	2228167
40764907	40764930	5	-	26627737	HCT116	viability after 18 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.15642344013665488	[153]	[73,92,29]	-1	hSpCas9	negative selection	2228168
40798090	40798113	5	-	26627737	HCT116	viability after 18 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.22819018109616773	[946]	[226,479,462]	-2	hSpCas9	negative selection	2228169
40777522	40777545	5	-	24336569	HL60	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.26645920279141055	[736]	[1875]	2	hSpCas9	negative selection	2286282
40777564	40777587	5	-	24336569	HL60	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	-0.03734154739658868	[804]	[1659]	0	hSpCas9	negative selection	2286283
40798058	40798081	5	+	24336569	HL60	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	-0.19655323599110863	[1238]	[2287]	-2	hSpCas9	negative selection	2286284
40798063	40798086	5	-	24336569	HL60	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	-1.289244651039193	[834]	[722]	-8	hSpCas9	negative selection	2286285
40798089	40798112	5	-	24336569	HL60	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.08019691122736639	[568]	[1138]	0	hSpCas9	negative selection	2286286
40798101	40798124	5	-	24336569	HL60	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	-0.5547034715047783	[1331]	[1918]	-5	hSpCas9	negative selection	2286287
40798130	40798153	5	+	24336569	HL60	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-0.02701631660651982	[142]	[296]	0	hSpCas9	negative selection	2286288
40798151	40798174	5	+	24336569	HL60	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	0.6397412081681413	[450]	[1486]	5	hSpCas9	negative selection	2286289
40798159	40798182	5	-	24336569	HL60	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	-0.545807128225656	[753]	[1092]	-5	hSpCas9	negative selection	2286290
40798169	40798192	5	+	24336569	HL60	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.958911661550321	[413]	[1702]	7	hSpCas9	negative selection	2286291
40777522	40777545	5	-	24336569	KBM7	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.33929427806952106	[678]	[2342]	3	hSpCas9	negative selection	2353459
40777564	40777587	5	-	24336569	KBM7	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	0.020798329851949182	[759]	[2102]	0	hSpCas9	negative selection	2353460
40798058	40798081	5	+	24336569	KBM7	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	0.9703597838157689	[682]	[3649]	8	hSpCas9	negative selection	2353461
40798063	40798086	5	-	24336569	KBM7	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	-1.190686233742996	[666]	[796]	-7	hSpCas9	negative selection	2353462
40798089	40798112	5	-	24336569	KBM7	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-1.7301097780084735	[595]	[489]	-8	hSpCas9	negative selection	2353463
40798101	40798124	5	-	24336569	KBM7	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	0.030106567854198296	[920]	[2564]	0	hSpCas9	negative selection	2353464
40798130	40798153	5	+	24336569	KBM7	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	1.745615584216277	[81]	[749]	9	hSpCas9	negative selection	2353465
40798151	40798174	5	+	24336569	KBM7	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	0.329723983961649	[394]	[1353]	3	hSpCas9	negative selection	2353466
40798159	40798182	5	-	24336569	KBM7	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	-0.12721161564913697	[554]	[1385]	-1	hSpCas9	negative selection	2353467
40798169	40798192	5	+	24336569	KBM7	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.11656714334106888	[604]	[1788]	1	hSpCas9	negative selection	2353468
40777036	40777059	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	GAGTTTCGCTTTTGTTGCCCAAG	PRKAA1	ENSG00000132356	-0.7818591031535113	[55,228]	[16,18]	-4	dCas9-VP64	positive selection	2393581
40783664	40783687	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGAGAATCGCTTGAACCCGAG	PRKAA1	ENSG00000132356	-1.1949452889942442	[147,110]	[6,17]	-7	dCas9-VP64	positive selection	2450079
40798271	40798294	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	AGGGCGGTGACTCGGCTCGGTGG	PRKAA1	ENSG00000132356	3.7009386772241295	[23,13]	[11,100]	9	dCas9-VP64	positive selection	2477087
40798365	40798388	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCGGGTACTGGTGATTCTCCTGG	PRKAA1	ENSG00000132356	1.4463335670476203	[251,381]	[24,369]	5	dCas9-VP64	positive selection	2477088
40798318	40798341	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	GGAGCGTCTGCGCCCGACGTAGG	PRKAA1	ENSG00000132356	1.1402799315098675	[61,150]	[70,30]	4	dCas9-VP64	positive selection	2477089
40777036	40777059	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	GAGTTTCGCTTTTGTTGCCCAAG	PRKAA1	ENSG00000132356	0.08408385798482881	[154,122]	[44,50]	0	dCas9-VP64	positive selection	2488836
40783664	40783687	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGAGAATCGCTTGAACCCGAG	PRKAA1	ENSG00000132356	1.2597172262171965	[381,372]	[459,141]	7	dCas9-VP64	positive selection	2545334
40798271	40798294	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	AGGGCGGTGACTCGGCTCGGTGG	PRKAA1	ENSG00000132356	-1.1220353938655265	[27,196]	[14,17]	-9	dCas9-VP64	positive selection	2572342
40798365	40798388	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCGGGTACTGGTGATTCTCCTGG	PRKAA1	ENSG00000132356	0.08174659167102483	[46,108]	[19,32]	0	dCas9-VP64	positive selection	2572343
40798318	40798341	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	GGAGCGTCTGCGCCCGACGTAGG	PRKAA1	ENSG00000132356	0.09415642061307494	[31,127]	[22,31]	0	dCas9-VP64	positive selection	2572344
40777505	40777528	5	-	27260157	DLD1	viability	ATTCGGAGCCTTGATGTGGTAGG	PRKAA1	ENSG00000132356	-0.0200146730736981	[]	[]	0	hSpCas9	negative selection	2573994
40798151	40798174	5	+	27260157	DLD1	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	0.584949569774623	[]	[]	8	hSpCas9	negative selection	2575093
40777522	40777545	5	-	27260157	DLD1	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.192319465444249	[]	[]	3	hSpCas9	negative selection	2577502
40771812	40771835	5	+	27260157	DLD1	viability	CCACACCAGAAAGGATCTGTTGG	PRKAA1	ENSG00000132356	-0.458387750534881	[]	[]	-4	hSpCas9	negative selection	2581388
40798058	40798081	5	+	27260157	DLD1	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	0.311374197248676	[]	[]	4	hSpCas9	negative selection	2583449
40777564	40777587	5	-	27260157	DLD1	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	0.106062827744907	[]	[]	3	hSpCas9	negative selection	2584159
40771798	40771821	5	-	27260157	DLD1	viability	CTGGTGTGGATTATTGTCACAGG	PRKAA1	ENSG00000132356	-0.115942360423861	[]	[]	-1	hSpCas9	negative selection	2584545
40798169	40798192	5	-	27260157	DLD1	viability	CCATGCGCAGACTCAGTTCCTGG	PRKAA1	ENSG00000132356	-0.169336003753926	[]	[]	-1	hSpCas9	negative selection	2584607
40798130	40798153	5	+	27260157	DLD1	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-0.0424126104296797	[]	[]	0	hSpCas9	negative selection	2587406
40775478	40775501	5	+	27260157	DLD1	viability	CAGATGGTGTACTGATGACCTGG	PRKAA1	ENSG00000132356	-0.636586018503573	[]	[]	-5	hSpCas9	negative selection	2606100
40798093	40798116	5	+	27260157	DLD1	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	0.0672136534425263	[]	[]	3	hSpCas9	negative selection	2608263
40798089	40798112	5	-	27260157	DLD1	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.293049019265207	[]	[]	-2	hSpCas9	negative selection	2609082
40775444	40775467	5	-	27260157	DLD1	viability	GTGATGGAATATGTCTCAGGAGG	PRKAA1	ENSG00000132356	-0.786184728266838	[]	[]	-6	hSpCas9	negative selection	2613374
40798063	40798086	5	-	27260157	DLD1	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	-0.654866480855303	[]	[]	-5	hSpCas9	negative selection	2615811
40771812	40771835	5	-	27260157	DLD1	viability	CCAACAGATCCTTTCTGGTGTGG	PRKAA1	ENSG00000132356	0.0471754179977389	[]	[]	3	hSpCas9	negative selection	2621699
40798159	40798182	5	-	27260157	DLD1	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	0.493264898474558	[]	[]	8	hSpCas9	negative selection	2644779
40798169	40798192	5	+	27260157	DLD1	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.636191319978219	[]	[]	8	hSpCas9	negative selection	2644848
40798087	40798110	5	-	27260157	DLD1	viability	CATTCTGGGTGACACGCTGGGGG	PRKAA1	ENSG00000132356	-1.11088679080292	[]	[]	-7	hSpCas9	negative selection	2645151
40771737	40771760	5	+	27260157	DLD1	viability	CATTCATGTGTGCATCAAGCAGG	PRKAA1	ENSG00000132356	-0.481123944078133	[]	[]	-4	hSpCas9	negative selection	2647361
40798101	40798124	5	-	27260157	DLD1	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	0.0610756988666172	[]	[]	3	hSpCas9	negative selection	2648113
40777505	40777528	5	-	27260157	HT1080	viability	ATTCGGAGCCTTGATGTGGTAGG	PRKAA1	ENSG00000132356	0.254084395430134	[]	[]	2	hSpCas9	negative selection	2650124
40798151	40798174	5	+	27260157	HT1080	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	-0.448321145494088	[]	[]	-7	hSpCas9	negative selection	2651223
40777522	40777545	5	-	27260157	HT1080	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.326228786085716	[]	[]	3	hSpCas9	negative selection	2653632
40771812	40771835	5	+	27260157	HT1080	viability	CCACACCAGAAAGGATCTGTTGG	PRKAA1	ENSG00000132356	-0.625793719347538	[]	[]	-8	hSpCas9	negative selection	2657518
40798058	40798081	5	+	27260157	HT1080	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	1.15247760791236	[]	[]	8	hSpCas9	negative selection	2659579
40777564	40777587	5	-	27260157	HT1080	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	-0.174909742580274	[]	[]	0	hSpCas9	negative selection	2660289
40771798	40771821	5	-	27260157	HT1080	viability	CTGGTGTGGATTATTGTCACAGG	PRKAA1	ENSG00000132356	-0.267261675853495	[]	[]	0	hSpCas9	negative selection	2660675
40798169	40798192	5	-	27260157	HT1080	viability	CCATGCGCAGACTCAGTTCCTGG	PRKAA1	ENSG00000132356	-0.0868691954451749	[]	[]	0	hSpCas9	negative selection	2660737
40798130	40798153	5	+	27260157	HT1080	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-0.885110200853448	[]	[]	-8	hSpCas9	negative selection	2663536
40775478	40775501	5	+	27260157	HT1080	viability	CAGATGGTGTACTGATGACCTGG	PRKAA1	ENSG00000132356	-0.601352241962002	[]	[]	-7	hSpCas9	negative selection	2682230
40798093	40798116	5	+	27260157	HT1080	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	-0.709111750218481	[]	[]	-8	hSpCas9	negative selection	2684393
40798089	40798112	5	-	27260157	HT1080	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.00586515881163348	[]	[]	0	hSpCas9	negative selection	2685212
40775444	40775467	5	-	27260157	HT1080	viability	GTGATGGAATATGTCTCAGGAGG	PRKAA1	ENSG00000132356	-0.160740738532704	[]	[]	0	hSpCas9	negative selection	2689504
40798063	40798086	5	-	27260157	HT1080	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	0.111821246449247	[]	[]	1	hSpCas9	negative selection	2691941
40771812	40771835	5	-	27260157	HT1080	viability	CCAACAGATCCTTTCTGGTGTGG	PRKAA1	ENSG00000132356	0.0939886831169762	[]	[]	1	hSpCas9	negative selection	2697829
40798159	40798182	5	-	27260157	HT1080	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	0.690326204424619	[]	[]	6	hSpCas9	negative selection	2720909
40798169	40798192	5	+	27260157	HT1080	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.724694223665727	[]	[]	6	hSpCas9	negative selection	2720978
40798087	40798110	5	-	27260157	HT1080	viability	CATTCTGGGTGACACGCTGGGGG	PRKAA1	ENSG00000132356	-0.617167540594263	[]	[]	-8	hSpCas9	negative selection	2721281
40771737	40771760	5	+	27260157	HT1080	viability	CATTCATGTGTGCATCAAGCAGG	PRKAA1	ENSG00000132356	0.803366882700954	[]	[]	7	hSpCas9	negative selection	2723491
40798101	40798124	5	-	27260157	HT1080	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	0.774128078254275	[]	[]	6	hSpCas9	negative selection	2724243
40777505	40777528	5	-	27260157	MKN45	viability	ATTCGGAGCCTTGATGTGGTAGG	PRKAA1	ENSG00000132356	0.124535150796647	[]	[]	4	hSpCas9	negative selection	2726254
40798151	40798174	5	+	27260157	MKN45	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	-0.832233458040409	[]	[]	-7	hSpCas9	negative selection	2727353
40777522	40777545	5	-	27260157	MKN45	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.816180127813131	[]	[]	8	hSpCas9	negative selection	2729762
40771812	40771835	5	+	27260157	MKN45	viability	CCACACCAGAAAGGATCTGTTGG	PRKAA1	ENSG00000132356	-0.369441124480248	[]	[]	-1	hSpCas9	negative selection	2733648
40798058	40798081	5	+	27260157	MKN45	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	0.848095373055397	[]	[]	8	hSpCas9	negative selection	2735709
40777564	40777587	5	-	27260157	MKN45	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	1.2660677901543	[]	[]	9	hSpCas9	negative selection	2736419
40771798	40771821	5	-	27260157	MKN45	viability	CTGGTGTGGATTATTGTCACAGG	PRKAA1	ENSG00000132356	0.717016450068068	[]	[]	7	hSpCas9	negative selection	2736805
40798169	40798192	5	-	27260157	MKN45	viability	CCATGCGCAGACTCAGTTCCTGG	PRKAA1	ENSG00000132356	0.359857572643344	[]	[]	6	hSpCas9	negative selection	2736867
40798130	40798153	5	+	27260157	MKN45	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-0.219550242165286	[]	[]	-1	hSpCas9	negative selection	2739666
40775478	40775501	5	+	27260157	MKN45	viability	CAGATGGTGTACTGATGACCTGG	PRKAA1	ENSG00000132356	0.182564083121636	[]	[]	5	hSpCas9	negative selection	2758360
40798093	40798116	5	+	27260157	MKN45	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	-0.141428875373979	[]	[]	0	hSpCas9	negative selection	2760523
40798089	40798112	5	-	27260157	MKN45	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.878390801360065	[]	[]	-7	hSpCas9	negative selection	2761342
40775444	40775467	5	-	27260157	MKN45	viability	GTGATGGAATATGTCTCAGGAGG	PRKAA1	ENSG00000132356	-0.574493837435179	[]	[]	-6	hSpCas9	negative selection	2765634
40798063	40798086	5	-	27260157	MKN45	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	-0.312375461364576	[]	[]	-1	hSpCas9	negative selection	2768071
40771812	40771835	5	-	27260157	MKN45	viability	CCAACAGATCCTTTCTGGTGTGG	PRKAA1	ENSG00000132356	0.591386550551039	[]	[]	7	hSpCas9	negative selection	2773959
40798159	40798182	5	-	27260157	MKN45	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	1.86546301714674	[]	[]	9	hSpCas9	negative selection	2797039
40798169	40798192	5	+	27260157	MKN45	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.918863247848199	[]	[]	8	hSpCas9	negative selection	2797108
40798087	40798110	5	-	27260157	MKN45	viability	CATTCTGGGTGACACGCTGGGGG	PRKAA1	ENSG00000132356	-0.297479415665644	[]	[]	-1	hSpCas9	negative selection	2797411
40771737	40771760	5	+	27260157	MKN45	viability	CATTCATGTGTGCATCAAGCAGG	PRKAA1	ENSG00000132356	1.46922986454014	[]	[]	9	hSpCas9	negative selection	2799621
40798101	40798124	5	-	27260157	MKN45	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	0.583100011650943	[]	[]	7	hSpCas9	negative selection	2800373
40777505	40777528	5	-	27260157	RKO	viability	ATTCGGAGCCTTGATGTGGTAGG	PRKAA1	ENSG00000132356	-0.747527928193253	[]	[]	-7	hSpCas9	negative selection	2802384
40798151	40798174	5	+	27260157	RKO	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	-0.0804978354856744	[]	[]	0	hSpCas9	negative selection	2803483
40777522	40777545	5	-	27260157	RKO	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.32675407966972	[]	[]	-6	hSpCas9	negative selection	2805892
40771812	40771835	5	+	27260157	RKO	viability	CCACACCAGAAAGGATCTGTTGG	PRKAA1	ENSG00000132356	-1.53914352578303	[]	[]	-9	hSpCas9	negative selection	2809778
40798058	40798081	5	+	27260157	RKO	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	0.05286760605438	[]	[]	0	hSpCas9	negative selection	2811839
40777564	40777587	5	-	27260157	RKO	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	-0.553902853249536	[]	[]	-7	hSpCas9	negative selection	2812549
40771798	40771821	5	-	27260157	RKO	viability	CTGGTGTGGATTATTGTCACAGG	PRKAA1	ENSG00000132356	-1.04567828723461	[]	[]	-8	hSpCas9	negative selection	2812935
40798169	40798192	5	-	27260157	RKO	viability	CCATGCGCAGACTCAGTTCCTGG	PRKAA1	ENSG00000132356	0.455787410729997	[]	[]	2	hSpCas9	negative selection	2812997
40798130	40798153	5	+	27260157	RKO	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-1.06253996112956	[]	[]	-8	hSpCas9	negative selection	2815796
40775478	40775501	5	+	27260157	RKO	viability	CAGATGGTGTACTGATGACCTGG	PRKAA1	ENSG00000132356	-1.84727297388458	[]	[]	-9	hSpCas9	negative selection	2834490
40798093	40798116	5	+	27260157	RKO	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	-2.31087985203696	[]	[]	-9	hSpCas9	negative selection	2836653
40798089	40798112	5	-	27260157	RKO	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.93894826373475	[]	[]	-8	hSpCas9	negative selection	2837472
40775444	40775467	5	-	27260157	RKO	viability	GTGATGGAATATGTCTCAGGAGG	PRKAA1	ENSG00000132356	-1.19317078218145	[]	[]	-8	hSpCas9	negative selection	2841764
40798063	40798086	5	-	27260157	RKO	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	-2.32730731636538	[]	[]	-9	hSpCas9	negative selection	2844201
40771812	40771835	5	-	27260157	RKO	viability	CCAACAGATCCTTTCTGGTGTGG	PRKAA1	ENSG00000132356	-1.35909500513918	[]	[]	-8	hSpCas9	negative selection	2850089
40798159	40798182	5	-	27260157	RKO	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	-0.0612971755234798	[]	[]	0	hSpCas9	negative selection	2873169
40798169	40798192	5	+	27260157	RKO	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.947373696987922	[]	[]	8	hSpCas9	negative selection	2873238
40798087	40798110	5	-	27260157	RKO	viability	CATTCTGGGTGACACGCTGGGGG	PRKAA1	ENSG00000132356	-2.06909248138001	[]	[]	-9	hSpCas9	negative selection	2873541
40771737	40771760	5	+	27260157	RKO	viability	CATTCATGTGTGCATCAAGCAGG	PRKAA1	ENSG00000132356	-0.998447126056266	[]	[]	-8	hSpCas9	negative selection	2875751
40798101	40798124	5	-	27260157	RKO	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	-1.44723065269534	[]	[]	-8	hSpCas9	negative selection	2876503
40777505	40777528	5	-	27260157	SF268	viability	ATTCGGAGCCTTGATGTGGTAGG	PRKAA1	ENSG00000132356	-0.389328650503278	[]	[]	-3	hSpCas9	negative selection	2878514
40798151	40798174	5	+	27260157	SF268	viability	GGCTGTCGCCATCTTTCTCCAGG	PRKAA1	ENSG00000132356	0.236104978166215	[]	[]	3	hSpCas9	negative selection	2879613
40777522	40777545	5	-	27260157	SF268	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.132871464776432	[]	[]	0	hSpCas9	negative selection	2882022
40771812	40771835	5	+	27260157	SF268	viability	CCACACCAGAAAGGATCTGTTGG	PRKAA1	ENSG00000132356	-0.209886702387949	[]	[]	-2	hSpCas9	negative selection	2885908
40798058	40798081	5	+	27260157	SF268	viability	CTCACCCTTCACTTTGCCGAAGG	PRKAA1	ENSG00000132356	0.473376983409947	[]	[]	7	hSpCas9	negative selection	2887969
40777564	40777587	5	-	27260157	SF268	viability	TTGGCAAACATGAATTGACTGGG	PRKAA1	ENSG00000132356	0.679093971789621	[]	[]	8	hSpCas9	negative selection	2888679
40771798	40771821	5	-	27260157	SF268	viability	CTGGTGTGGATTATTGTCACAGG	PRKAA1	ENSG00000132356	-0.0707005075397214	[]	[]	0	hSpCas9	negative selection	2889065
40798169	40798192	5	-	27260157	SF268	viability	CCATGCGCAGACTCAGTTCCTGG	PRKAA1	ENSG00000132356	0.648658158150146	[]	[]	8	hSpCas9	negative selection	2889127
40798130	40798153	5	+	27260157	SF268	viability	CCCGTCGTGTTTCTGCTTCTCGG	PRKAA1	ENSG00000132356	-0.261512364886165	[]	[]	-2	hSpCas9	negative selection	2891926
40775478	40775501	5	+	27260157	SF268	viability	CAGATGGTGTACTGATGACCTGG	PRKAA1	ENSG00000132356	0.0956082047899069	[]	[]	0	hSpCas9	negative selection	2910620
40798093	40798116	5	+	27260157	SF268	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	-0.279656366313649	[]	[]	-2	hSpCas9	negative selection	2912783
40798089	40798112	5	-	27260157	SF268	viability	TACATTCTGGGTGACACGCTGGG	PRKAA1	ENSG00000132356	-0.559570622904491	[]	[]	-4	hSpCas9	negative selection	2913602
40775444	40775467	5	-	27260157	SF268	viability	GTGATGGAATATGTCTCAGGAGG	PRKAA1	ENSG00000132356	-0.175249694385627	[]	[]	-1	hSpCas9	negative selection	2917894
40798063	40798086	5	-	27260157	SF268	viability	CGGCACCTTCGGCAAAGTGAAGG	PRKAA1	ENSG00000132356	0.458828367593793	[]	[]	7	hSpCas9	negative selection	2920331
40771812	40771835	5	-	27260157	SF268	viability	CCAACAGATCCTTTCTGGTGTGG	PRKAA1	ENSG00000132356	0.190201862787041	[]	[]	0	hSpCas9	negative selection	2926219
40798159	40798182	5	-	27260157	SF268	viability	ACTCAGTTCCTGGAGAAAGATGG	PRKAA1	ENSG00000132356	1.33852401241414	[]	[]	9	hSpCas9	negative selection	2949299
40798169	40798192	5	+	27260157	SF268	viability	CCAGGAACTGAGTCTGCGCATGG	PRKAA1	ENSG00000132356	0.358943947971228	[]	[]	4	hSpCas9	negative selection	2949368
40798087	40798110	5	-	27260157	SF268	viability	CATTCTGGGTGACACGCTGGGGG	PRKAA1	ENSG00000132356	-0.21561695816108	[]	[]	-2	hSpCas9	negative selection	2949671
40771737	40771760	5	+	27260157	SF268	viability	CATTCATGTGTGCATCAAGCAGG	PRKAA1	ENSG00000132356	0.30898702309126	[]	[]	4	hSpCas9	negative selection	2951881
40798101	40798124	5	-	27260157	SF268	viability	AAGATCGGCCACTACATTCTGGG	PRKAA1	ENSG00000132356	0.817783211062278	[]	[]	8	hSpCas9	negative selection	2952633
40798127	40798150	5	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	0.11533210897078758	[4,4]	[3,4]	2	hSpCas9	positive selection	3013603
40767671	40767694	5	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.4002419430945854	[4,4]	[4,5]	8	hSpCas9	positive selection	3013604
40798131	40798154	5	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.5829729469508289	[1,3]	[2,0]	-7	hSpCas9	positive selection	3013605
40777477	40777500	5	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	0.08962031365166756	[5,5]	[5,4]	1	hSpCas9	positive selection	3013606
40798127	40798150	5	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	0.19915190847594985	[4,4]	[4,2]	3	hSpCas9	positive selection	3081461
40767671	40767694	5	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.543363732620666	[4,4]	[6,4]	8	hSpCas9	positive selection	3081462
40798131	40798154	5	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	0.14568653566783407	[1,1]	[2,0]	2	hSpCas9	positive selection	3081463
40777477	40777500	5	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	0.3905369346312133	[5,5]	[6,5]	6	hSpCas9	positive selection	3081464
40767503	40767526		+	24336571	A375	resistance to PLX after 7 days	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	0.14603271360239095	[12,11]	[13,13]	5	hSpCas9	positive selection	3137721
40767655	40767678		+	24336571	A375	resistance to PLX after 7 days	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.3415627532058787	[54,54]	[43,40]	-8	hSpCas9	positive selection	3137722
40777565	40777588		-	24336571	A375	resistance to PLX after 7 days	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.3370232978249358	[22,21]	[16,18]	-8	hSpCas9	positive selection	3137723
40777522	40777545		-	24336571	A375	resistance to PLX after 7 days	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.11096804098487723	[18,21]	[21,22]	3	hSpCas9	positive selection	3137724
40767679	40767702		-	24336571	A375	resistance to PLX after 7 days	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.31486360273596115	[29,29]	[23,23]	-8	hSpCas9	positive selection	3137725
40777465	40777488		-	24336571	A375	resistance to PLX after 7 days	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	-0.09566405520833687	[24,24]	[22,22]	-3	hSpCas9	positive selection	3137726
40767503	40767526		+	24336571	A375	resistance to PLX after 14 days	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	0.8245352639859433	[8,14]	[9,9]	7	hSpCas9	positive selection	3195514
40767655	40767678		+	24336571	A375	resistance to PLX after 14 days	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.8688342264393478	[49,44]	[9,12]	-8	hSpCas9	positive selection	3195515
40777565	40777588		-	24336571	A375	resistance to PLX after 14 days	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.48507710966549356	[17,19]	[6,4]	-6	hSpCas9	positive selection	3195516
40777522	40777545		-	24336571	A375	resistance to PLX after 14 days	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.8664577723695807	[16,28]	[20,18]	7	hSpCas9	positive selection	3195517
40767679	40767702		-	24336571	A375	resistance to PLX after 14 days	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.7028122167982278	[23,32]	[8,6]	-7	hSpCas9	positive selection	3195518
40777465	40777488		-	24336571	A375	resistance to PLX after 14 days	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	-0.20508602888601335	[19,30]	[9,9]	-2	hSpCas9	positive selection	3195519
40767503	40767526		+	24336571	A375	viability after 7 days	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	-0.52283596287008	[18]	[12,11]	-6	hSpCas9	negative selection	3253307
40767655	40767678		+	24336571	A375	viability after 7 days	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.03278838931093597	[55]	[54,54]	0	hSpCas9	negative selection	3253308
40777565	40777588		-	24336571	A375	viability after 7 days	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.19392086903530104	[26]	[22,21]	-3	hSpCas9	negative selection	3253309
40777522	40777545		-	24336571	A375	viability after 7 days	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.235306413887381	[17]	[18,21]	3	hSpCas9	negative selection	3253310
40767679	40767702		-	24336571	A375	viability after 7 days	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.6150651505825461	[19]	[29,29]	8	hSpCas9	negative selection	3253311
40777465	40777488		-	24336571	A375	viability after 7 days	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	0.08917526547183718	[24]	[24,24]	1	hSpCas9	negative selection	3253312
40767503	40767526		+	24336571	A375	viability after 14 days	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	-0.5337989196340746	[18]	[8,14]	-6	hSpCas9	negative selection	3311100
40767655	40767678		+	24336571	A375	viability after 14 days	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.14020185367540994	[55]	[49,44]	-2	hSpCas9	negative selection	3311101
40777565	40777588		-	24336571	A375	viability after 14 days	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.4426884665090527	[26]	[17,19]	-5	hSpCas9	negative selection	3311102
40777522	40777545		-	24336571	A375	viability after 14 days	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.4021934628388984	[17]	[16,28]	5	hSpCas9	negative selection	3311103
40767679	40767702		-	24336571	A375	viability after 14 days	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.5617830623006245	[19]	[23,32]	7	hSpCas9	negative selection	3311104
40777465	40777488		-	24336571	A375	viability after 14 days	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	0.14165882861156176	[24]	[19,30]	1	hSpCas9	negative selection	3311105
40777565	40777588	5	-	27383988	293T	resistance to West Nile virus (flavivirus)	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	2.61347002167111	[24,24]	[0,0]	8	hSpCas9	positive selection	3387437
40774930	40774953	5	-	27383988	293T	resistance to West Nile virus (flavivirus)	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	5.764286200165718	[75,75]	[26,26]	9	hSpCas9	positive selection	3387438
40774956	40774979	5	-	27383988	293T	resistance to West Nile virus (flavivirus)	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.851198245332334	[275,275]	[0,0]	-6	hSpCas9	positive selection	3387439
40798127	40798150	5	-	26780180	HT29	viability (Avana library 4 designs)	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	1.20548684035553	[]	[]	5	hSpCas9	negative selection	3451788
40767671	40767694	5	-	26780180	HT29	viability (Avana library 4 designs)	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.227813587174031	[]	[]	-4	hSpCas9	negative selection	3451789
40798131	40798154	5	-	26780180	HT29	viability (Avana library 4 designs)	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	1.48478887805854	[]	[]	6	hSpCas9	negative selection	3451790
40777476	40777499	5	+	26780180	HT29	viability (Avana library 4 designs)	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	-0.756689818037557	[]	[]	-8	hSpCas9	negative selection	3451791
40777476	40777499	5	+	26780180	A375	viability (Avana lentiCRISPRv2)	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	0.453238676077516	[]	[]	0	hSpCas9	negative selection	3547852
40767671	40767694	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	3.66916867308489	[]	[]	6	hSpCas9	negative selection	3547853
40798127	40798150	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	0.758489133426547	[]	[]	1	hSpCas9	negative selection	3547854
40798131	40798154	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-2.06834634826506	[]	[]	-3	hSpCas9	negative selection	3547855
40767485	40767508	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-6.88881322401791	[]	[]	-6	hSpCas9	negative selection	3547856
40775409	40775432	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	TTATATCTGTAAGAATGGAAGGG	PRKAA1	ENSG00000132356	-22.2916197172895	[]	[]	-9	hSpCas9	negative selection	3547857
40777476	40777499	5	+	26780180	A375	viability (Avana lentiGuide)	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	-0.870955791588622	[]	[]	-1	hSpCas9	negative selection	3656511
40767671	40767694	5	-	26780180	A375	viability (Avana lentiGuide)	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	1.89336454855149	[]	[]	2	hSpCas9	negative selection	3656512
40798127	40798150	5	-	26780180	A375	viability (Avana lentiGuide)	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	10.6716018003386	[]	[]	7	hSpCas9	negative selection	3656513
40798131	40798154	5	-	26780180	A375	viability (Avana lentiGuide)	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-2.00324059314354	[]	[]	-3	hSpCas9	negative selection	3656514
40767485	40767508	5	-	26780180	A375	viability (Avana lentiGuide)	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	5.14348842760372	[]	[]	4	hSpCas9	negative selection	3656515
40775409	40775432	5	-	26780180	A375	viability (Avana lentiGuide)	TTATATCTGTAAGAATGGAAGGG	PRKAA1	ENSG00000132356	1.5666448483098	[]	[]	1	hSpCas9	negative selection	3656516
40777476	40777499	5	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	-0.008595213522721468	[7]	[7,6]	0	hSpCas9	positive selection	3765118
40767671	40767694	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.13403829963763	[5]	[6,5]	3	hSpCas9	positive selection	3765119
40798127	40798150	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	0.03311142374931027	[5]	[6,4]	0	hSpCas9	positive selection	3765120
40798131	40798154	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.3312246363059851	[4]	[3,3]	-5	hSpCas9	positive selection	3765121
40767485	40767508	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.3596868203447381	[4]	[3,3]	-5	hSpCas9	positive selection	3765122
40775409	40775432	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TTATATCTGTAAGAATGGAAGGG	PRKAA1	ENSG00000132356	-1.2003692120883889	[10]	[3,4]	-9	hSpCas9	positive selection	3765123
40777476	40777499	5	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGAGGTTCTGAATTTCTCTGCGG	PRKAA1	ENSG00000132356	0.19738092293425974	[7]	[6,7]	5	hSpCas9	positive selection	3873725
40767671	40767694	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.21088013536316652	[6]	[6,5]	5	hSpCas9	positive selection	3873726
40798127	40798150	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	AGAAGCAGAAACACGACGGGCGG	PRKAA1	ENSG00000132356	-0.38713810768180534	[6]	[3,3]	-5	hSpCas9	positive selection	3873727
40798131	40798154	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.08097196825810968	[5]	[5,2]	-1	hSpCas9	positive selection	3873728
40767485	40767508	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.2420889290600181	[5]	[4,3]	-3	hSpCas9	positive selection	3873729
40775409	40775432	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TTATATCTGTAAGAATGGAAGGG	PRKAA1	ENSG00000132356	0.1193223968315025	[6]	[5,6]	2	hSpCas9	positive selection	3873730
40774930	40774953	5	-	26780180	A375	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	1.00107759058116	[]	[]	4	hSpCas9	negative selection	3984177
40767465	40767488	5	-	26780180	A375	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.50788740188315	[]	[]	1	hSpCas9	negative selection	3984178
40777565	40777588	5	-	26780180	A375	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.708372536264349	[]	[]	2	hSpCas9	negative selection	3984179
40777522	40777545	5	-	26780180	A375	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.541162268194725	[]	[]	1	hSpCas9	negative selection	3984180
40767679	40767702	5	-	26780180	A375	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	1.7393009421205	[]	[]	9	hSpCas9	negative selection	3984181
40774956	40774979	5	-	26780180	A375	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	1.07642878623038	[]	[]	5	hSpCas9	negative selection	3984182
40774930	40774953	5	-	26780180	HT29	viability (GeCKOv2 library)	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	1.26529896635329	[]	[]	6	hSpCas9	negative selection	4092143
40767465	40767488	5	-	26780180	HT29	viability (GeCKOv2 library)	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.734355370018315	[]	[]	3	hSpCas9	negative selection	4092144
40777565	40777588	5	-	26780180	HT29	viability (GeCKOv2 library)	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.87576603151688	[]	[]	3	hSpCas9	negative selection	4092145
40777522	40777545	5	-	26780180	HT29	viability (GeCKOv2 library)	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.382935066785877	[]	[]	1	hSpCas9	negative selection	4092146
40767679	40767702	5	-	26780180	HT29	viability (GeCKOv2 library)	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.813692397348779	[]	[]	3	hSpCas9	negative selection	4092147
40774956	40774979	5	-	26780180	HT29	viability (GeCKOv2 library)	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	1.1913975416104	[]	[]	6	hSpCas9	negative selection	4092148
40767503	40767526	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	0.07376664567256691	[3]	[1,3]	1	hSpCas9	positive selection	4162876
40767655	40767678	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.061678368905648484	[5]	[4,3]	1	hSpCas9	positive selection	4162877
40777565	40777588	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.12534766432898714	[4]	[2,2]	-3	hSpCas9	positive selection	4162878
40777522	40777545	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.4244001888626191	[3]	[3,4]	8	hSpCas9	positive selection	4162879
40767679	40767702	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.008160425287267042	[4]	[2,2]	0	hSpCas9	positive selection	4162880
40777465	40777488	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	0.12473692959063923	[4]	[2,3]	3	hSpCas9	positive selection	4162881
40767503	40767526	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCTGCAGCATATGTTTCAAAAGG	PRKAA1	ENSG00000132356	0.9645948555993036	[4]	[4,4,1,3]	6	hSpCas9	positive selection	4226898
40767655	40767678	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CTGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	1.0137798561237559	[5]	[4,4,2,5]	7	hSpCas9	positive selection	4226899
40777565	40777588	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.9427247559761489	[5]	[3,4,5,3]	6	hSpCas9	positive selection	4226900
40777522	40777545	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.3830415655666831	[3]	[3,1,4,0]	3	hSpCas9	positive selection	4226901
40767679	40767702	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.9652712536040133	[3]	[0,2,4,3]	6	hSpCas9	positive selection	4226902
40777465	40777488	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TTCAGAACCTCAAGCTTTTCAGG	PRKAA1	ENSG00000132356	1.1718795290893431	[3]	[4,4,1,4]	8	hSpCas9	positive selection	4226903
40777522	40777545	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.031917140811226874	[0]	[0,0]	0	hSpCas9	positive selection	4292126
40767679	40767702	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.33940465028262545	[4]	[3,1]	-6	hSpCas9	positive selection	4292127
40767465	40767488	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.7188227629751625	[1]	[0,3]	9	hSpCas9	positive selection	4292128
40777565	40777588	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.3256584443896551	[1]	[0,0]	-6	hSpCas9	positive selection	4356384
40774930	40774953	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	0.016976167943329096	[2]	[1,2]	0	hSpCas9	positive selection	4356385
40774956	40774979	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.03783400676043036	[4]	[4,2]	0	hSpCas9	positive selection	4356386
40767656	40767678	5	+	27760321	OCIAML3	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-2.109186430940693	[324,265]	[51,54]	-9	hSpCas9	negative selection	4430526
40767671	40767693	5	-	27760321	OCIAML3	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-1.0454048958899096	[543,446]	[129,260]	-7	hSpCas9	negative selection	4430527
40769415	40769437	5	-	27760321	OCIAML3	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	-3.0041851019042687	[156,120]	[22,0]	-9	hSpCas9	negative selection	4430528
40775486	40775508	5	+	27760321	OCIAML3	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-1.233007979741019	[509,432]	[207,88]	-8	hSpCas9	negative selection	4430529
40798062	40798084	5	-	27760321	OCIAML3	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.00353006404577684	[495,342]	[298,358]	0	hSpCas9	negative selection	4430530
40767656	40767678	5	+	27760321	OCIAML2	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.382321215582874	[324,265]	[109,277]	-4	hSpCas9	negative selection	4513989
40767671	40767693	5	-	27760321	OCIAML2	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.5564322794634002	[543,446]	[231,322]	-6	hSpCas9	negative selection	4513990
40769415	40769437	5	-	27760321	OCIAML2	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	-0.4057912969011106	[156,120]	[88,77]	-5	hSpCas9	negative selection	4513991
40775486	40775508	5	+	27760321	OCIAML2	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-0.4634435713720926	[509,432]	[279,269]	-5	hSpCas9	negative selection	4513992
40798062	40798084	5	-	27760321	OCIAML2	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	-0.4324079154262237	[495,342]	[232,268]	-5	hSpCas9	negative selection	4513993
40767656	40767678	5	+	27760321	MV411	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.10297563700371237	[324,265]	[207,208]	0	hSpCas9	negative selection	4597452
40767671	40767693	5	-	27760321	MV411	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.15607692761405836	[543,446]	[372,346]	1	hSpCas9	negative selection	4597453
40769415	40769437	5	-	27760321	MV411	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	-1.526606221147424	[156,120]	[35,24]	-7	hSpCas9	negative selection	4597454
40775486	40775508	5	+	27760321	MV411	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-0.6444098485575104	[509,432]	[167,243]	-4	hSpCas9	negative selection	4597455
40798062	40798084	5	-	27760321	MV411	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	-0.18235629070363246	[495,342]	[129,407]	-1	hSpCas9	negative selection	4597456
40767656	40767678	5	+	27760321	HL60	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.7330318110756304	[324,265]	[144,143]	-6	hSpCas9	negative selection	4680915
40767671	40767693	5	-	27760321	HL60	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	1.5793113295942869	[543,446]	[1312,1087]	9	hSpCas9	negative selection	4680916
40769415	40769437	5	-	27760321	HL60	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	-1.2993780690096828	[156,120]	[38,52]	-8	hSpCas9	negative selection	4680917
40775486	40775508	5	+	27760321	HL60	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-1.3087079187567283	[509,432]	[202,103]	-8	hSpCas9	negative selection	4680918
40798062	40798084	5	-	27760321	HL60	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	-1.3647271875655547	[495,342]	[164,95]	-8	hSpCas9	negative selection	4680919
40767656	40767678	5	+	27760321	HT1080	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.7356336767216565	[265,207]	[39,74]	-3	hSpCas9	negative selection	4764378
40767671	40767693	5	-	27760321	HT1080	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.14371898733082236	[446,348]	[283,55]	0	hSpCas9	negative selection	4764379
40769415	40769437	5	-	27760321	HT1080	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	1.324583212927216	[120,94]	[92,123]	7	hSpCas9	negative selection	4764380
40775486	40775508	5	+	27760321	HT1080	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.732887942481941	[432,337]	[217,295]	4	hSpCas9	negative selection	4764381
40798062	40798084	5	-	27760321	HT1080	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.49470893033473956	[342,267]	[65,286]	2	hSpCas9	negative selection	4764382
40767656	40767678	5	+	27760321	HT29	viability after 7 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.15534631406285115	[265,207]	[255,352,394]	2	hSpCas9	negative selection	4847841
40767671	40767693	5	-	27760321	HT29	viability after 7 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.2282251171107503	[446,348]	[731,696,393]	3	hSpCas9	negative selection	4847842
40769415	40769437	5	-	27760321	HT29	viability after 7 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.4341387073579108	[120,94]	[339,104,140]	6	hSpCas9	negative selection	4847843
40775486	40775508	5	+	27760321	HT29	viability after 7 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.040962129609425676	[432,337]	[644,574,333]	0	hSpCas9	negative selection	4847844
40798062	40798084	5	-	27760321	HT29	viability after 7 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.4753256447765045	[342,267]	[566,638,437]	7	hSpCas9	negative selection	4847845
40767656	40767678	5	+	27760321	HT29	viability after 25 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.3128386914244362	[265,207]	[310,401,292]	3	hSpCas9	negative selection	4931304
40767671	40767693	5	-	27760321	HT29	viability after 25 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.4657335876097205	[446,348]	[573,804,493]	5	hSpCas9	negative selection	4931305
40769415	40769437	5	-	27760321	HT29	viability after 25 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.8494275824927765	[120,94]	[223,202,241]	8	hSpCas9	negative selection	4931306
40775486	40775508	5	+	27760321	HT29	viability after 25 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.47695034635234096	[432,337]	[600,455,801]	5	hSpCas9	negative selection	4931307
40798062	40798084	5	-	27760321	HT29	viability after 25 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.7538892089322398	[342,267]	[427,565,803]	8	hSpCas9	negative selection	4931308
40767656	40767678	5	+	27760321	HT29	viability after 22 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.10215420674630893	[265,207]	[116,457,193]	-1	hSpCas9	negative selection	5014767
40767671	40767693	5	-	27760321	HT29	viability after 22 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.29631229995142594	[446,348]	[709,534,444]	3	hSpCas9	negative selection	5014768
40769415	40769437	5	-	27760321	HT29	viability after 22 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.6412413242430905	[120,94]	[264,174,141]	7	hSpCas9	negative selection	5014769
40775486	40775508	5	+	27760321	HT29	viability after 22 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.8171189956177964	[432,337]	[745,787,822]	8	hSpCas9	negative selection	5014770
40798062	40798084	5	-	27760321	HT29	viability after 22 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.8984823484156912	[342,267]	[545,585,847]	9	hSpCas9	negative selection	5014771
40767656	40767678	5	+	27760321	HT29	viability after 19 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.4517608663379158	[265,207]	[458,578,93]	4	hSpCas9	negative selection	5098230
40767671	40767693	5	-	27760321	HT29	viability after 19 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.28606899874915337	[446,348]	[609,546,514]	2	hSpCas9	negative selection	5098231
40769415	40769437	5	-	27760321	HT29	viability after 19 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	1.0089513937696644	[120,94]	[193,267,276]	9	hSpCas9	negative selection	5098232
40775486	40775508	5	+	27760321	HT29	viability after 19 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.6305736209852472	[432,337]	[775,462,812]	6	hSpCas9	negative selection	5098233
40798062	40798084	5	-	27760321	HT29	viability after 19 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.8930224415721146	[342,267]	[544,463,915]	8	hSpCas9	negative selection	5098234
40767656	40767678	5	+	27760321	HT29	viability after 16 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.35507077135248144	[265,207]	[340,326,349]	4	hSpCas9	negative selection	5181693
40767671	40767693	5	-	27760321	HT29	viability after 16 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.08501960760023194	[446,348]	[685,398,353]	0	hSpCas9	negative selection	5181694
40769415	40769437	5	-	27760321	HT29	viability after 16 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.821196085943545	[120,94]	[172,245,216]	8	hSpCas9	negative selection	5181695
40775486	40775508	5	+	27760321	HT29	viability after 16 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.2355326206846058	[432,337]	[605,567,356]	2	hSpCas9	negative selection	5181696
40798062	40798084	5	-	27760321	HT29	viability after 16 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	1.1483330255851978	[342,267]	[803,958,508]	9	hSpCas9	negative selection	5181697
40767656	40767678	5	+	27760321	HT29	viability after 13 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.2658775844330235	[265,207]	[371,293,325]	3	hSpCas9	negative selection	5265156
40767671	40767693	5	-	27760321	HT29	viability after 13 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.03808939306561791	[446,348]	[476,422,448]	0	hSpCas9	negative selection	5265157
40769415	40769437	5	-	27760321	HT29	viability after 13 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.6315990145107624	[120,94]	[205,156,217]	7	hSpCas9	negative selection	5265158
40775486	40775508	5	+	27760321	HT29	viability after 13 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	0.24484228039861716	[432,337]	[368,603,611]	3	hSpCas9	negative selection	5265159
40798062	40798084	5	-	27760321	HT29	viability after 13 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.7832776389349583	[342,267]	[713,579,539]	8	hSpCas9	negative selection	5265160
40767656	40767678	5	+	27760321	HT29	viability after 10 days	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	0.1955470122581136	[265,207]	[267,517,237]	2	hSpCas9	negative selection	5348619
40767671	40767693	5	-	27760321	HT29	viability after 10 days	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.24192642939518164	[446,348]	[594,786,390]	3	hSpCas9	negative selection	5348620
40769415	40769437	5	-	27760321	HT29	viability after 10 days	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	0.10670585242853448	[120,94]	[137,133,162]	1	hSpCas9	negative selection	5348621
40775486	40775508	5	+	27760321	HT29	viability after 10 days	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-0.02758407978299139	[432,337]	[483,455,475]	0	hSpCas9	negative selection	5348622
40798062	40798084	5	-	27760321	HT29	viability after 10 days	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	0.37904101424047415	[342,267]	[473,602,415]	5	hSpCas9	negative selection	5348623
40767656	40767678	5	+	27760321	MOLM13	viability	TGCTCCATATATCTACCTCTGG	PRKAA1	ENSG00000132356	-0.8784340471294254	[324,265]	[12,155]	-5	hSpCas9	negative selection	5432082
40767671	40767693	5	-	27760321	MOLM13	viability	AGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-2.557985231225079	[543,446]	[90,9]	-8	hSpCas9	negative selection	5432083
40769415	40769437	5	-	27760321	MOLM13	viability	ACCAGAAGTAATTTCAGGAAGG	PRKAA1	ENSG00000132356	-1.1270391483768223	[156,120]	[26,42]	-6	hSpCas9	negative selection	5432084
40775486	40775508	5	+	27760321	MOLM13	viability	GTACTGATGACCTGGTACCTGG	PRKAA1	ENSG00000132356	-2.3029728880175635	[509,432]	[43,61]	-8	hSpCas9	negative selection	5432085
40798062	40798084	5	-	27760321	MOLM13	viability	GCACCTTCGGCAAAGTGAAGGG	PRKAA1	ENSG00000132356	-1.9588932831843633	[495,342]	[30,84]	-8	hSpCas9	negative selection	5432086
40797918	40797941	5	+	27661255	K562	viability	GGAGAGCTCCCGCAGGATCCGGG	PRKAA1	ENSG00000132356	0.0622832277590028	[807,1048]	[1016,560]	2	dCas9-KRAB	negative selection	5504096
40798032	40798055	5	+	27661255	K562	viability	GGGGCCAAGCCTAGTCCCGCGGG	PRKAA1	ENSG00000132356	0.008354695614150698	[1991,2074]	[1759,1523]	0	dCas9-KRAB	negative selection	5504097
40798093	40798116	5	+	27661255	K562	viability	GCGTGTCACCCAGAATGTAGTGG	PRKAA1	ENSG00000132356	0.07488464316755089	[875,858]	[795,673]	2	dCas9-KRAB	negative selection	5504098
40797702	40797725	5	-	27661255	K562	viability	GCCGGGCGCGATCCCCGGGGAGG	PRKAA1	ENSG00000132356	0.06620352674602681	[200,279]	[193,204]	2	dCas9-KRAB	negative selection	5504099
40797705	40797728	5	-	27661255	K562	viability	GGCGCCGGGCGCGATCCCCGGGG	PRKAA1	ENSG00000132356	0.12219057403784611	[1059,1066]	[1025,837]	4	dCas9-KRAB	negative selection	5504100
40777522	40777545	5	-	27260156	BXPC3	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.162433678086916	[57]	[21,70,96,88]	3	hSpCas9	negative selection	5564586
40767679	40767702	5	-	27260156	BXPC3	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.12865995006275766	[1732]	[1457,1657,1814,1718]	-3	hSpCas9	negative selection	5564587
40767465	40767488	5	-	27260156	BXPC3	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.30060039717301335	[104]	[138,100,175,124]	6	hSpCas9	negative selection	5564588
40777565	40777588	5	-	27260156	BXPC3	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.13571422328896876	[66]	[41,95,51,64]	-3	hSpCas9	negative selection	5628844
40774930	40774953	5	-	27260156	BXPC3	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.6111264028814343	[355]	[240,253,237,238]	-8	hSpCas9	negative selection	5628845
40774956	40774979	5	-	27260156	BXPC3	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.1040679733399481	[992]	[814,938,966,1180]	-2	hSpCas9	negative selection	5628846
40777522	40777545	5	-	27260156	A673	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.09686275362306462	[57]	[64,57,78,57]	-2	hSpCas9	negative selection	5685837
40767679	40767702	5	-	27260156	A673	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.13737938074098666	[1732]	[2108,1638,1921,1926]	-3	hSpCas9	negative selection	5685838
40767465	40767488	5	-	27260156	A673	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.2607307585319407	[104]	[172,111,145,177]	6	hSpCas9	negative selection	5685839
40777565	40777588	5	-	27260156	A673	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.22091412197603866	[66]	[65,98,102,103]	5	hSpCas9	negative selection	5750095
40774930	40774953	5	-	27260156	A673	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.35757780304677694	[355]	[340,294,385,311]	-7	hSpCas9	negative selection	5750096
40774956	40774979	5	-	27260156	A673	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.14942391075950257	[992]	[1006,1023,1189,1068]	-4	hSpCas9	negative selection	5750097
40777522	40777545	5	-	27260156	A375	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.2764469460838299	[57]	[7,31,203,76]	4	hSpCas9	negative selection	5807088
40767679	40767702	5	-	27260156	A375	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.2010514336098177	[1732]	[1661,2165,1807,1488]	-3	hSpCas9	negative selection	5807089
40767465	40767488	5	-	27260156	A375	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.4039419966858555	[104]	[228,150,184,90]	6	hSpCas9	negative selection	5807090
40777565	40777588	5	-	27260156	A375	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.5068163692255472	[66]	[57,221,147,39]	7	hSpCas9	negative selection	5871346
40774930	40774953	5	-	27260156	A375	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.7440454311292954	[355]	[286,210,266,224]	-7	hSpCas9	negative selection	5871347
40774956	40774979	5	-	27260156	A375	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.07655777531848074	[992]	[1338,1350,975,801]	-1	hSpCas9	negative selection	5871348
40777522	40777545	5	-	27260156	COLO741	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.16914404957330698	[57]	[83,75,40]	-4	hSpCas9	negative selection	5928339
40767679	40767702	5	-	27260156	COLO741	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.0756253066613195	[1732]	[2644,2225,2162]	1	hSpCas9	negative selection	5928340
40767465	40767488	5	-	27260156	COLO741	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.43044476468410364	[104]	[66,104,121]	-7	hSpCas9	negative selection	5928341
40777565	40777588	5	-	27260156	COLO741	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.6250280716614856	[66]	[12,95,53]	-8	hSpCas9	negative selection	5992597
40774930	40774953	5	-	27260156	COLO741	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.4807205416102686	[355]	[330,228,410]	-7	hSpCas9	negative selection	5992598
40774956	40774979	5	-	27260156	COLO741	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.11169813877908086	[992]	[1283,1430,839]	-2	hSpCas9	negative selection	5992599
40777522	40777545	5	-	27260156	CAL120	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.4310766456838442	[57]	[102,50,137]	6	hSpCas9	negative selection	6049590
40767679	40767702	5	-	27260156	CAL120	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.2647108755772578	[1732]	[2253,1607,1594]	-4	hSpCas9	negative selection	6049591
40767465	40767488	5	-	27260156	CAL120	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.008802138493490208	[104]	[57,149,169]	0	hSpCas9	negative selection	6049592
40777565	40777588	5	-	27260156	CAL120	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-1.315308276470489	[66]	[30,28,39]	-9	hSpCas9	negative selection	6113848
40774930	40774953	5	-	27260156	CAL120	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.5852013189105731	[355]	[445,255,207]	-7	hSpCas9	negative selection	6113849
40774956	40774979	5	-	27260156	CAL120	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.05805668931666075	[992]	[1319,1277,985]	-1	hSpCas9	negative selection	6113850
40777522	40777545	5	-	27260156	CADOES1	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.18048091608415096	[57]	[34,33,32,89]	-3	hSpCas9	negative selection	6170841
40767679	40767702	5	-	27260156	CADOES1	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.10819416494917755	[1732]	[1443,1714,1693,2110]	1	hSpCas9	negative selection	6170842
40767465	40767488	5	-	27260156	CADOES1	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.28828317941832005	[104]	[130,46,78,61]	-5	hSpCas9	negative selection	6170843
40777565	40777588	5	-	27260156	CADOES1	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.4781402099756731	[66]	[34,74,106,131]	7	hSpCas9	negative selection	6235099
40774930	40774953	5	-	27260156	CADOES1	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.5762664295638449	[355]	[178,206,186,318]	-7	hSpCas9	negative selection	6235100
40774956	40774979	5	-	27260156	CADOES1	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.17598979776531265	[992]	[1048,1102,916,1106]	3	hSpCas9	negative selection	6235101
40777522	40777545	5	-	27260156	EWS502	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.11290856082531109	[57]	[25,31,66,152]	2	hSpCas9	negative selection	6292092
40767679	40767702	5	-	27260156	EWS502	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.06762169777113902	[1732]	[1650,1931,1746,1667]	-1	hSpCas9	negative selection	6292093
40767465	40767488	5	-	27260156	EWS502	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.140910145319336	[104]	[77,167,150,94]	2	hSpCas9	negative selection	6292094
40777565	40777588	5	-	27260156	EWS502	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.31043883850713705	[66]	[50,56,56,64]	-5	hSpCas9	negative selection	6356350
40774930	40774953	5	-	27260156	EWS502	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.3383485759130452	[355]	[351,214,275,348]	-5	hSpCas9	negative selection	6356351
40774956	40774979	5	-	27260156	EWS502	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.2041140756937243	[992]	[1049,1386,1158,1260]	3	hSpCas9	negative selection	6356352
40777522	40777545	5	-	27260156	EW8	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.22537651383603663	[57]	[41,25,38,53]	-4	hSpCas9	negative selection	6413343
40767679	40767702	5	-	27260156	EW8	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.09393944252371811	[1732]	[1480,1302,1119,1406]	-2	hSpCas9	negative selection	6413344
40767465	40767488	5	-	27260156	EW8	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.4602311826175529	[104]	[46,36,111,40]	-7	hSpCas9	negative selection	6413345
40777565	40777588	5	-	27260156	EW8	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.2885350114656968	[66]	[37,60,50,25]	-5	hSpCas9	negative selection	6477601
40774930	40774953	5	-	27260156	EW8	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.45091698650686457	[355]	[215,247,189,193]	-7	hSpCas9	negative selection	6477602
40774956	40774979	5	-	27260156	EW8	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.03946159357457568	[992]	[926,726,828,832]	0	hSpCas9	negative selection	6477603
40777522	40777545	5	-	27260156	CORL105	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.38312268676992245	[57]	[37,85,25,52]	-6	hSpCas9	negative selection	6534594
40767679	40767702	5	-	27260156	CORL105	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.23049402039517175	[1732]	[1904,1527,2021,1517]	-4	hSpCas9	negative selection	6534595
40767465	40767488	5	-	27260156	CORL105	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.017558082100361805	[104]	[79,116,198,111]	0	hSpCas9	negative selection	6534596
40777565	40777588	5	-	27260156	CORL105	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.9267571706261046	[66]	[42,41,21,54]	-9	hSpCas9	negative selection	6598852
40774930	40774953	5	-	27260156	CORL105	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.41534520828725346	[355]	[375,254,360,268]	-6	hSpCas9	negative selection	6598853
40774956	40774979	5	-	27260156	CORL105	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.061946810243652006	[992]	[1144,907,1272,1157]	-1	hSpCas9	negative selection	6598854
40777522	40777545	5	-	27260156	HS294T	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	1.6194577742098042	[57]	[57,38,23,173]	9	hSpCas9	negative selection	6655845
40767679	40767702	5	-	27260156	HS294T	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.19247156807565347	[1732]	[936,655,654,805]	2	hSpCas9	negative selection	6655846
40767465	40767488	5	-	27260156	HS294T	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-1.0106800544221932	[104]	[6,13,40,19]	-9	hSpCas9	negative selection	6655847
40777565	40777588	5	-	27260156	HS294T	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.7890091211332715	[66]	[8,19,17,12]	-8	hSpCas9	negative selection	6720103
40774930	40774953	5	-	27260156	HS294T	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.5125585503133141	[355]	[127,85,86,78]	-6	hSpCas9	negative selection	6720104
40774956	40774979	5	-	27260156	HS294T	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.33858073390305177	[992]	[315,494,367,835]	4	hSpCas9	negative selection	6720105
40777522	40777545	5	-	27260156	HCC44	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.1926785909070574	[57]	[55,28,160,72]	3	hSpCas9	negative selection	6777096
40767679	40767702	5	-	27260156	HCC44	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.0407817869726006	[1732]	[2169,1642,2759,1681]	0	hSpCas9	negative selection	6777097
40767465	40767488	5	-	27260156	HCC44	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.22363367562845715	[104]	[118,159,118,146]	3	hSpCas9	negative selection	6777098
40777565	40777588	5	-	27260156	HCC44	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.04963932754400968	[66]	[23,143,63,44]	-1	hSpCas9	negative selection	6841354
40774930	40774953	5	-	27260156	HCC44	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.6865780702666959	[355]	[337,164,323,205]	-7	hSpCas9	negative selection	6841355
40774956	40774979	5	-	27260156	HCC44	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.040944152913779064	[992]	[1692,794,1026,933]	0	hSpCas9	negative selection	6841356
40777522	40777545	5	-	27260156	G402	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.20981684997189198	[57]	[104,80,46,20]	-3	hSpCas9	negative selection	6898347
40767679	40767702	5	-	27260156	G402	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.06685144972238288	[1732]	[1915,1989,1997,2140]	-1	hSpCas9	negative selection	6898348
40767465	40767488	5	-	27260156	G402	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.28916754637359254	[104]	[182,17,177,48]	-4	hSpCas9	negative selection	6898349
40777565	40777588	5	-	27260156	G402	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.21917766907476155	[66]	[103,67,6,195]	2	hSpCas9	negative selection	6962605
40774930	40774953	5	-	27260156	G402	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.006574039682802346	[355]	[442,336,596,350]	0	hSpCas9	negative selection	6962606
40774956	40774979	5	-	27260156	G402	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.146811923316295	[992]	[1194,1388,745,1075]	-2	hSpCas9	negative selection	6962607
40777522	40777545	5	-	27260156	L33	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.5773878552121134	[57]	[36,10,50,10]	-8	hSpCas9	negative selection	7019598
40767679	40767702	5	-	27260156	L33	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.22271153634851415	[1732]	[1424,682,896,1422]	-5	hSpCas9	negative selection	7019599
40767465	40767488	5	-	27260156	L33	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.07327296530109617	[104]	[110,25,107,95]	1	hSpCas9	negative selection	7019600
40777565	40777588	5	-	27260156	L33	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.4598559352542311	[66]	[57,18,30,40]	-8	hSpCas9	negative selection	7083856
40774930	40774953	5	-	27260156	L33	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.18212558275163931	[355]	[264,134,175,380]	-4	hSpCas9	negative selection	7083857
40774956	40774979	5	-	27260156	L33	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.04289993631618261	[992]	[1020,366,644,911]	-1	hSpCas9	negative selection	7083858
40777522	40777545	5	-	27260156	K562	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.5796290892544875	[57]	[98,83,53,72]	7	hSpCas9	negative selection	7140849
40767679	40767702	5	-	27260156	K562	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.49379028885961596	[1732]	[977,1357,934,1096]	-5	hSpCas9	negative selection	7140850
40767465	40767488	5	-	27260156	K562	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.4819166907369321	[104]	[88,182,122,124]	6	hSpCas9	negative selection	7140851
40777565	40777588	5	-	27260156	K562	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.18376413554019555	[66]	[7,136,59,9]	-2	hSpCas9	negative selection	7205107
40774930	40774953	5	-	27260156	K562	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	0.12037754684978164	[355]	[365,206,743,80]	1	hSpCas9	negative selection	7205108
40774956	40774979	5	-	27260156	K562	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.07814294920879528	[992]	[1277,713,913,838]	0	hSpCas9	negative selection	7205109
40777522	40777545	5	-	27260156	HT29	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.6551240572417316	[57]	[113,146,86,99]	9	hSpCas9	negative selection	7262100
40767679	40767702	5	-	27260156	HT29	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.10292955190336284	[1732]	[2493,2156,2029,2349]	2	hSpCas9	negative selection	7262101
40767465	40767488	5	-	27260156	HT29	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.08446042332349624	[104]	[136,134,151,65]	-2	hSpCas9	negative selection	7262102
40777565	40777588	5	-	27260156	HT29	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.02231893863656076	[66]	[46,25,105,127]	0	hSpCas9	negative selection	7326358
40774930	40774953	5	-	27260156	HT29	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.2925178487838653	[355]	[317,466,361,279]	-5	hSpCas9	negative selection	7326359
40774956	40774979	5	-	27260156	HT29	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.1327283066506766	[992]	[1099,1111,1174,1019]	-3	hSpCas9	negative selection	7326360
40777522	40777545	5	-	27260156	MHHES1	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.2860512454700572	[57]	[50,13,39,93]	-5	hSpCas9	negative selection	7383351
40767679	40767702	5	-	27260156	MHHES1	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.03991285757091689	[1732]	[2462,2008,2206,1481]	0	hSpCas9	negative selection	7383352
40767465	40767488	5	-	27260156	MHHES1	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.25654669311086575	[104]	[175,40,65,110]	-5	hSpCas9	negative selection	7383353
40777565	40777588	5	-	27260156	MHHES1	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.6553976520445945	[66]	[80,44,24,43]	-8	hSpCas9	negative selection	7447609
40774930	40774953	5	-	27260156	MHHES1	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	0.07962662310887614	[355]	[344,591,508,280]	1	hSpCas9	negative selection	7447610
40774956	40774979	5	-	27260156	MHHES1	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.11642741291617975	[992]	[1051,1507,1160,1129]	2	hSpCas9	negative selection	7447611
40777522	40777545	5	-	27260156	MEWO	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.8655506744149983	[57]	[79,83,128]	9	hSpCas9	negative selection	7504602
40767679	40767702	5	-	27260156	MEWO	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.01869373537691099	[1732]	[1943,1511,1411]	0	hSpCas9	negative selection	7504603
40767465	40767488	5	-	27260156	MEWO	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.374546379274729	[104]	[119,48,66]	-7	hSpCas9	negative selection	7504604
40777565	40777588	5	-	27260156	MEWO	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.889861914251032	[66]	[30,18,50]	-9	hSpCas9	negative selection	7568860
40774930	40774953	5	-	27260156	MEWO	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.6139339699121298	[355]	[211,163,275]	-8	hSpCas9	negative selection	7568861
40774956	40774979	5	-	27260156	MEWO	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.13230849818727286	[992]	[914,781,858]	-3	hSpCas9	negative selection	7568862
40777522	40777545	5	-	27260156	LNCAPCLONEFGC	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.4626384613853243	[57]	[63,68,65,89]	7	hSpCas9	negative selection	7625853
40767679	40767702	5	-	27260156	LNCAPCLONEFGC	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.08243337279804952	[1732]	[1551,1668,1761,1680]	1	hSpCas9	negative selection	7625854
40767465	40767488	5	-	27260156	LNCAPCLONEFGC	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.6563716741211081	[104]	[275,87,87,139]	9	hSpCas9	negative selection	7625855
40777565	40777588	5	-	27260156	LNCAPCLONEFGC	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.8817422422864403	[66]	[42,19,63,9]	-8	hSpCas9	negative selection	7690111
40774930	40774953	5	-	27260156	LNCAPCLONEFGC	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.579894354860427	[355]	[283,156,266,164]	-7	hSpCas9	negative selection	7690112
40774956	40774979	5	-	27260156	LNCAPCLONEFGC	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.12839240330594326	[992]	[732,694,1096,816]	-2	hSpCas9	negative selection	7690113
40777522	40777545	5	-	27260156	PANC0327	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.17394319326938623	[57]	[35,31,56,108]	2	hSpCas9	negative selection	7747104
40767679	40767702	5	-	27260156	PANC0327	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.1643894817070004	[1732]	[1669,1067,1418,1400]	-2	hSpCas9	negative selection	7747105
40767465	40767488	5	-	27260156	PANC0327	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.8198834520482081	[104]	[141,50,51,425]	9	hSpCas9	negative selection	7747106
40777565	40777588	5	-	27260156	PANC0327	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.1800381883199451	[66]	[24,73,13,91]	-3	hSpCas9	negative selection	7811362
40774930	40774953	5	-	27260156	PANC0327	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.4858809401027429	[355]	[280,222,181,221]	-6	hSpCas9	negative selection	7811363
40774956	40774979	5	-	27260156	PANC0327	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.05756016081578608	[992]	[1015,682,935,1074]	0	hSpCas9	negative selection	7811364
40777522	40777545	5	-	27260156	NCIH2009	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-1.5731262308368184	[57]	[32,15,33]	-9	hSpCas9	negative selection	7868355
40767679	40767702	5	-	27260156	NCIH2009	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.22192055703168556	[1732]	[2467,1916,1919]	-3	hSpCas9	negative selection	7868356
40767465	40767488	5	-	27260156	NCIH2009	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.6714232521981285	[104]	[66,103,99]	-7	hSpCas9	negative selection	7868357
40777565	40777588	5	-	27260156	NCIH2009	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.7652298226968695	[66]	[28,53,76]	-7	hSpCas9	negative selection	7932613
40774930	40774953	5	-	27260156	NCIH2009	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.349545288934669	[355]	[399,408,362]	-5	hSpCas9	negative selection	7932614
40774956	40774979	5	-	27260156	NCIH2009	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.14142716580538273	[992]	[1524,1155,1144]	-2	hSpCas9	negative selection	7932615
40777522	40777545	5	-	27260156	NCIH1373	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.7014531449009169	[57]	[32,48,61,2]	-6	hSpCas9	negative selection	7989606
40767679	40767702	5	-	27260156	NCIH1373	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.12517870931786934	[1732]	[2417,1831,2099,576]	1	hSpCas9	negative selection	7989607
40767465	40767488	5	-	27260156	NCIH1373	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.05534044004342631	[104]	[124,82,204,9]	0	hSpCas9	negative selection	7989608
40777565	40777588	5	-	27260156	NCIH1373	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.7236834733422848	[66]	[73,184,166,12]	7	hSpCas9	negative selection	8053864
40774930	40774953	5	-	27260156	NCIH1373	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	0.14573921315136606	[355]	[175,433,872,56]	1	hSpCas9	negative selection	8053865
40774956	40774979	5	-	27260156	NCIH1373	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.5288737394865008	[992]	[1180,1746,2088,337]	5	hSpCas9	negative selection	8053866
40777522	40777545	5	-	27260156	PATU8902	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	1.1431229787739334	[57]	[266,266,140,27]	9	hSpCas9	negative selection	8110857
40767679	40767702	5	-	27260156	PATU8902	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.05912393685662831	[1732]	[3064,2415,2038,2649]	0	hSpCas9	negative selection	8110858
40767465	40767488	5	-	27260156	PATU8902	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.12112148520507438	[104]	[315,240,10,95]	1	hSpCas9	negative selection	8110859
40777565	40777588	5	-	27260156	PATU8902	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.11153731107503617	[66]	[168,16,114,95]	1	hSpCas9	negative selection	8175115
40774930	40774953	5	-	27260156	PATU8902	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.8255817893664368	[355]	[60,351,376,307]	-6	hSpCas9	negative selection	8175116
40774956	40774979	5	-	27260156	PATU8902	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.35129401790654335	[992]	[1752,2996,1059,1372]	3	hSpCas9	negative selection	8175117
40777522	40777545	5	-	27260156	PANC1	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.4266723430460502	[57]	[37,30,50,27]	-6	hSpCas9	negative selection	8232108
40767679	40767702	5	-	27260156	PANC1	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.13386681561728397	[1732]	[1563,1798,1043,1119]	-2	hSpCas9	negative selection	8232109
40767465	40767488	5	-	27260156	PANC1	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.2279347224226369	[104]	[44,210,71,113]	3	hSpCas9	negative selection	8232110
40777565	40777588	5	-	27260156	PANC1	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.7187864329352558	[66]	[120,174,51,51]	9	hSpCas9	negative selection	8296366
40774930	40774953	5	-	27260156	PANC1	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.18876586590318462	[355]	[281,175,344,249]	-3	hSpCas9	negative selection	8296367
40774956	40774979	5	-	27260156	PANC1	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.1119246567845702	[992]	[906,1251,462,636]	-2	hSpCas9	negative selection	8296368
40777522	40777545	5	-	27260156	PANC0813	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-1.255380876898907	[57]	[17,19,59,15]	-9	hSpCas9	negative selection	8353359
40767679	40767702	5	-	27260156	PANC0813	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.0973755287377931	[1732]	[1964,2025,1842,1785]	-2	hSpCas9	negative selection	8353360
40767465	40767488	5	-	27260156	PANC0813	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.9325115403060882	[104]	[58,106,32,58]	-8	hSpCas9	negative selection	8353361
40777565	40777588	5	-	27260156	PANC0813	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.5454295947579726	[66]	[122,223,76,39]	8	hSpCas9	negative selection	8417617
40774930	40774953	5	-	27260156	PANC0813	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.1514233732702574	[355]	[464,253,334,454]	-2	hSpCas9	negative selection	8417618
40774956	40774979	5	-	27260156	PANC0813	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.277334960771916	[992]	[1613,1513,1070,1466]	4	hSpCas9	negative selection	8417619
40777522	40777545	5	-	27260156	RDES	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.3755305778787017	[57]	[52,24,71,57]	-6	hSpCas9	negative selection	8474610
40767679	40767702	5	-	27260156	RDES	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.16353686667841494	[1732]	[1647,1491,1903,2130]	-3	hSpCas9	negative selection	8474611
40767465	40767488	5	-	27260156	RDES	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.1613852929096038	[104]	[135,110,108,188]	2	hSpCas9	negative selection	8474612
40777565	40777588	5	-	27260156	RDES	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.8038986707369573	[66]	[34,25,83,33]	-8	hSpCas9	negative selection	8538868
40774930	40774953	5	-	27260156	RDES	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.6571438823388944	[355]	[181,248,353,268]	-8	hSpCas9	negative selection	8538869
40774956	40774979	5	-	27260156	RDES	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.06951515306174261	[992]	[1015,1168,1403,1219]	1	hSpCas9	negative selection	8538870
40777522	40777545	5	-	27260156	PC3	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.6674458940552279	[57]	[41,62,315,48]	9	hSpCas9	negative selection	8595861
40767679	40767702	5	-	27260156	PC3	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.13048554898490994	[1732]	[1041,1341,2517,1877]	-3	hSpCas9	negative selection	8595862
40767465	40767488	5	-	27260156	PC3	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.14288802070655482	[104]	[46,144,81,198]	3	hSpCas9	negative selection	8595863
40777565	40777588	5	-	27260156	PC3	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.7908501901568497	[66]	[27,35,50,44]	-9	hSpCas9	negative selection	8660119
40774930	40774953	5	-	27260156	PC3	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.38232025149695353	[355]	[142,292,330,381]	-7	hSpCas9	negative selection	8660120
40774956	40774979	5	-	27260156	PC3	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.1791315152121431	[992]	[564,884,1595,774]	-4	hSpCas9	negative selection	8660121
40777522	40777545	5	-	27260156	PATU8988T	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.016762857542187426	[57]	[92,40,26,59]	0	hSpCas9	negative selection	8717112
40767679	40767702	5	-	27260156	PATU8988T	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.1128697512164083	[1732]	[1707,1254,1419,1805]	-2	hSpCas9	negative selection	8717113
40767465	40767488	5	-	27260156	PATU8988T	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.09400160231145449	[104]	[44,165,122,103]	1	hSpCas9	negative selection	8717114
40777565	40777588	5	-	27260156	PATU8988T	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	0.07613797677504008	[66]	[117,97,29,26]	1	hSpCas9	negative selection	8781370
40774930	40774953	5	-	27260156	PATU8988T	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.25214836215138575	[355]	[374,390,144,252]	-4	hSpCas9	negative selection	8781371
40774956	40774979	5	-	27260156	PATU8988T	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.041019733638160805	[992]	[1465,951,824,679]	0	hSpCas9	negative selection	8781372
40777522	40777545	5	-	27260156	T47D	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.4418311385859992	[57]	[122,67,73,104]	8	hSpCas9	negative selection	8838363
40767679	40767702	5	-	27260156	T47D	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.1059199153618342	[1732]	[1999,1489,2084,1961]	-2	hSpCas9	negative selection	8838364
40767465	40767488	5	-	27260156	T47D	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.1213989312101873	[104]	[190,114,107,119]	2	hSpCas9	negative selection	8838365
40777565	40777588	5	-	27260156	T47D	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.31319737808667014	[66]	[69,52,47,81]	-6	hSpCas9	negative selection	8902621
40774930	40774953	5	-	27260156	T47D	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.3426161628034648	[355]	[297,352,281,374]	-6	hSpCas9	negative selection	8902622
40774956	40774979	5	-	27260156	T47D	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.06862431061191954	[992]	[1154,1033,1041,1190]	-1	hSpCas9	negative selection	8902623
40777522	40777545	5	-	27260156	SU8686	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.07439603770231784	[57]	[40,35,95,77]	1	hSpCas9	negative selection	8959614
40767679	40767702	5	-	27260156	SU8686	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.07300713757824728	[1732]	[1546,1505,1663,1869]	-1	hSpCas9	negative selection	8959615
40767465	40767488	5	-	27260156	SU8686	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.3080567770156324	[104]	[59,72,92,119]	-5	hSpCas9	negative selection	8959616
40777565	40777588	5	-	27260156	SU8686	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.08178582992314132	[66]	[38,66,61,88]	-1	hSpCas9	negative selection	9023872
40774930	40774953	5	-	27260156	SU8686	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.1658912628785349	[355]	[356,292,271,333]	-3	hSpCas9	negative selection	9023873
40774956	40774979	5	-	27260156	SU8686	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.03651298351134913	[992]	[836,947,1174,1121]	0	hSpCas9	negative selection	9023874
40777522	40777545	5	-	27260156	SKES1	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.16343053941576668	[57]	[41,98,57,60]	3	hSpCas9	negative selection	9080865
40767679	40767702	5	-	27260156	SKES1	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.09278934967746588	[1732]	[1718,1888,1431,1490]	-2	hSpCas9	negative selection	9080866
40767465	40767488	5	-	27260156	SKES1	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.15738002831898656	[104]	[39,131,88,108]	-3	hSpCas9	negative selection	9080867
40777565	40777588	5	-	27260156	SKES1	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.7946347304241268	[66]	[80,34,8,34]	-9	hSpCas9	negative selection	9145123
40774930	40774953	5	-	27260156	SKES1	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.6785733601674595	[355]	[238,344,222,108]	-9	hSpCas9	negative selection	9145124
40774956	40774979	5	-	27260156	SKES1	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.021541010579814168	[992]	[971,1164,912,877]	0	hSpCas9	negative selection	9145125
40777522	40777545	5	-	27260156	TOV112D	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.9970668539554479	[57]	[80,85,232,54]	9	hSpCas9	negative selection	9202116
40767679	40767702	5	-	27260156	TOV112D	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.09340918724886249	[1732]	[2387,1811,1480,1760]	1	hSpCas9	negative selection	9202117
40767465	40767488	5	-	27260156	TOV112D	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.15064821161104325	[104]	[111,78,182,83]	3	hSpCas9	negative selection	9202118
40777565	40777588	5	-	27260156	TOV112D	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.49125011473422875	[66]	[18,17,18,122]	-7	hSpCas9	negative selection	9266374
40774930	40774953	5	-	27260156	TOV112D	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.5300825720079605	[355]	[391,289,186,143]	-8	hSpCas9	negative selection	9266375
40774956	40774979	5	-	27260156	TOV112D	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.2122204045392223	[992]	[1024,742,894,757]	-4	hSpCas9	negative selection	9266376
40777522	40777545	5	-	27260156	TC71	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	-0.07512081155218997	[57]	[23,58,60,94]	-2	hSpCas9	negative selection	9323367
40767679	40767702	5	-	27260156	TC71	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	-0.08602691256432315	[1732]	[1511,1742,1691,2080]	-2	hSpCas9	negative selection	9323368
40767465	40767488	5	-	27260156	TC71	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	-0.2993685074088406	[104]	[55,97,136,72]	-7	hSpCas9	negative selection	9323369
40777565	40777588	5	-	27260156	TC71	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.21186163623450938	[66]	[81,45,64,53]	-5	hSpCas9	negative selection	9387625
40774930	40774953	5	-	27260156	TC71	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.38064447250093475	[355]	[291,251,344,280]	-8	hSpCas9	negative selection	9387626
40774956	40774979	5	-	27260156	TC71	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	-0.2532777877264021	[992]	[807,997,791,984]	-6	hSpCas9	negative selection	9387627
40777522	40777545	5	-	27260156	TC32	viability	TACTCAATCGACAGAAGATTCGG	PRKAA1	ENSG00000132356	0.1650700599665189	[57]	[51,67,55,61]	3	hSpCas9	negative selection	9444618
40767679	40767702	5	-	27260156	TC32	viability	TCCTGTTACAGATTGTATGCAGG	PRKAA1	ENSG00000132356	0.04078369411508631	[1732]	[1607,1320,2101,1790]	0	hSpCas9	negative selection	9444619
40767465	40767488	5	-	27260156	TC32	viability	GGGCCACAATCAAAGATATCAGG	PRKAA1	ENSG00000132356	0.3921669883431578	[104]	[107,134,116,148]	6	hSpCas9	negative selection	9444620
40777565	40777588	5	-	27260156	TC32	viability	GTTGGCAAACATGAATTGACTGG	PRKAA1	ENSG00000132356	-0.8622353809001022	[66]	[35,0,48,67]	-9	hSpCas9	negative selection	9508876
40774930	40774953	5	-	27260156	TC32	viability	AGTAAAAACAGGCTCCACGAAGG	PRKAA1	ENSG00000132356	-0.33218719011110615	[355]	[221,247,410,198]	-6	hSpCas9	negative selection	9508877
40774956	40774979	5	-	27260156	TC32	viability	TTTCAGAAATCTGATGTACCTGG	PRKAA1	ENSG00000132356	0.0972845229578636	[992]	[864,767,1316,1151]	1	hSpCas9	negative selection	9508878
40762842	40762865	5	+	27869803	HPAFII	viability after 27 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.46652326368936436	[110]	[66,91,NaN]	4	hSpCas9	negative selection	9591915
40762943	40762966	5	-	27869803	HPAFII	viability after 27 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.6833096878437782	[149]	[88,157,NaN]	5	hSpCas9	negative selection	9591916
40764907	40764930	5	-	27869803	HPAFII	viability after 27 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.41059138082918917	[162]	[180,57,NaN]	3	hSpCas9	negative selection	9591917
40798090	40798113	5	-	27869803	HPAFII	viability after 27 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.5075871468654415	[432]	[261,374,NaN]	4	hSpCas9	negative selection	9591918
40762842	40762865	5	+	27869803	HPAFII	viability after 15 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.5440258842648517	[110]	[205,153,NaN]	6	hSpCas9	negative selection	9674364
40762943	40762966	5	-	27869803	HPAFII	viability after 15 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.7536761914053879	[149]	[404,188,NaN]	8	hSpCas9	negative selection	9674365
40764907	40764930	5	-	27869803	HPAFII	viability after 15 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.3197549506252676	[162]	[357,131,NaN]	3	hSpCas9	negative selection	9674366
40798090	40798113	5	-	27869803	HPAFII	viability after 15 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.6948940483811082	[432]	[986,607,NaN]	7	hSpCas9	negative selection	9674367
40762842	40762865	5	+	27869803	ASPC1	viability after 36 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.44882234369854235	[247]	[312,91,0]	4	hSpCas9	negative selection	9756813
40762943	40762966	5	-	27869803	ASPC1	viability after 36 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.26239802582320815	[576]	[99,372,50]	-3	hSpCas9	negative selection	9756814
40764907	40764930	5	-	27869803	ASPC1	viability after 36 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.23818935109353	[412]	[103,136,121]	-2	hSpCas9	negative selection	9756815
40798090	40798113	5	-	27869803	ASPC1	viability after 36 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.8225826341346798	[1515]	[1222,1063,622]	7	hSpCas9	negative selection	9756816
40762842	40762865	5	+	27869803	PATU8988S	viability after 35 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.17775504332995662	[215]	[54,141,NaN]	2	hSpCas9	negative selection	9839262
40762943	40762966	5	-	27869803	PATU8988S	viability after 35 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	-0.43228219835676085	[367]	[38,180,NaN]	-4	hSpCas9	negative selection	9839263
40764907	40764930	5	-	27869803	PATU8988S	viability after 35 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	-0.06417563323521647	[304]	[114,119,NaN]	0	hSpCas9	negative selection	9839264
40798090	40798113	5	-	27869803	PATU8988S	viability after 35 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.022754397222917833	[1231]	[454,521,NaN]	0	hSpCas9	negative selection	9839265
40762842	40762865	5	+	27869803	PATU8988S	viability after 31 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.196203173125423	[215]	[77,124,NaN]	2	hSpCas9	negative selection	9921711
40762943	40762966	5	-	27869803	PATU8988S	viability after 31 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.0381216970870224	[367]	[85,232,NaN]	0	hSpCas9	negative selection	9921712
40764907	40764930	5	-	27869803	PATU8988S	viability after 31 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.2899151878536247	[304]	[171,118,NaN]	3	hSpCas9	negative selection	9921713
40798090	40798113	5	-	27869803	PATU8988S	viability after 31 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	-0.12377826937135605	[1231]	[361,563,NaN]	-1	hSpCas9	negative selection	9921714
40762842	40762865	5	+	27869803	HPAFII	viability after 35 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.5380441410716	[110]	[90,70,NaN]	3	hSpCas9	negative selection	10004160
40762943	40762966	5	-	27869803	HPAFII	viability after 35 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.40361213153106196	[149]	[59,145,NaN]	2	hSpCas9	negative selection	10004161
40764907	40764930	5	-	27869803	HPAFII	viability after 35 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.7004280192648662	[162]	[198,60,NaN]	4	hSpCas9	negative selection	10004162
40798090	40798113	5	-	27869803	HPAFII	viability after 35 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.2774102974683289	[432]	[212,324,NaN]	1	hSpCas9	negative selection	10004163
40762842	40762865	5	+	27869803	HPAFII	viability after 31 days	GGTCTTGGAGTTAGGTCAACAGG	PRKAA1	ENSG00000132356	0.5049297978292212	[110]	[74,102,NaN]	3	hSpCas9	negative selection	10086609
40762943	40762966	5	-	27869803	HPAFII	viability after 31 days	GCAACTATCGATCTTGCCAAAGG	PRKAA1	ENSG00000132356	0.9329709915809254	[149]	[120,203,NaN]	7	hSpCas9	negative selection	10086610
40764907	40764930	5	-	27869803	HPAFII	viability after 31 days	CTTGGTTGCTGAAACACCAAGGG	PRKAA1	ENSG00000132356	0.46568020210891214	[162]	[155,93,NaN]	3	hSpCas9	negative selection	10086611
40798090	40798113	5	-	27869803	HPAFII	viability after 31 days	CTACATTCTGGGTGACACGCTGG	PRKAA1	ENSG00000132356	0.3022481582181141	[432]	[246,356,NaN]	2	hSpCas9	negative selection	10086612
40777509	40777532	5	-	28162770	P31FUJ	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.794360100800591	[213]	[144]	-6	hSpCas9	negative selection	10216910
40764858	40764881	5	-	28162770	P31FUJ	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.3147134022009962	[307]	[290]	-3	hSpCas9	negative selection	10216911
40767652	40767675	5	-	28162770	P31FUJ	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.1274492042681499	[131]	[70]	-7	hSpCas9	negative selection	10216912
40798131	40798154	5	-	28162770	P31FUJ	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.0559244423302996	[22]	[12]	-6	hSpCas9	negative selection	10216913
40798088	40798111	5	-	28162770	P31FUJ	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.0272180707644845	[332]	[191]	-6	hSpCas9	negative selection	10216914
40767485	40767508	5	-	28162770	P31FUJ	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.0704199651893294	[276]	[154]	-6	hSpCas9	negative selection	10216915
40777458	40777481	5	+	28162770	P31FUJ	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.0749225446326593	[146]	[81]	-6	hSpCas9	negative selection	10216916
40771788	40771811	5	-	28162770	P31FUJ	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.509693410923571	[543]	[448]	-4	hSpCas9	negative selection	10216917
40767671	40767694	5	-	28162770	P31FUJ	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.4595730662614008	[604]	[516]	-4	hSpCas9	negative selection	10216918
40764912	40764935	5	+	28162770	P31FUJ	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.7174447603885217	[176]	[341]	7	hSpCas9	negative selection	10216919
40777509	40777532	5	-	28162770	TF1	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[90]	1	hSpCas9	negative selection	10386276
40764858	40764881	5	-	28162770	TF1	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[266]	1	hSpCas9	negative selection	10386277
40767652	40767675	5	-	28162770	TF1	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[28]	1	hSpCas9	negative selection	10386278
40798131	40798154	5	-	28162770	TF1	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[18]	1	hSpCas9	negative selection	10386279
40798088	40798111	5	-	28162770	TF1	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[203]	1	hSpCas9	negative selection	10386280
40767485	40767508	5	-	28162770	TF1	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[79]	1	hSpCas9	negative selection	10386281
40777458	40777481	5	+	28162770	TF1	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[195]	1	hSpCas9	negative selection	10386282
40771788	40771811	5	-	28162770	TF1	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[312]	1	hSpCas9	negative selection	10386283
40767671	40767694	5	-	28162770	TF1	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[382]	1	hSpCas9	negative selection	10386284
40764912	40764935	5	+	28162770	TF1	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	NaN	[NaN]	[130]	1	hSpCas9	negative selection	10386285
40777509	40777532	5	-	28162770	SKM1	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-1.3708949440356402	[78]	[53]	-7	hSpCas9	negative selection	10555642
40764858	40764881	5	-	28162770	SKM1	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-1.9453841135272876	[60]	[27]	-8	hSpCas9	negative selection	10555643
40767652	40767675	5	-	28162770	SKM1	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.2090248211312524	[33]	[25]	-7	hSpCas9	negative selection	10555644
40798131	40798154	5	-	28162770	SKM1	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.8220016980220053	[7]	[7]	-6	hSpCas9	negative selection	10555645
40798088	40798111	5	-	28162770	SKM1	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.583214838434889	[99]	[58]	-8	hSpCas9	negative selection	10555646
40767485	40767508	5	-	28162770	SKM1	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.2854022188622485	[90]	[65]	-7	hSpCas9	negative selection	10555647
40777458	40777481	5	+	28162770	SKM1	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.28766527037081735	[28]	[41]	-2	hSpCas9	negative selection	10555648
40771788	40771811	5	-	28162770	SKM1	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.8220016980220056	[82]	[82]	-6	hSpCas9	negative selection	10555649
40767671	40767694	5	-	28162770	SKM1	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.9151111024134868	[127]	[119]	-6	hSpCas9	negative selection	10555650
40764912	40764935	5	+	28162770	SKM1	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.5356975128653644	[40]	[49]	-4	hSpCas9	negative selection	10555651
40777509	40777532	5	-	28162770	SKM1	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-1.3708949440356402	[78]	[53]	-7	hSpCas9	negative selection	10725008
40764858	40764881	5	-	28162770	SKM1	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-1.9453841135272876	[60]	[27]	-8	hSpCas9	negative selection	10725009
40767652	40767675	5	-	28162770	SKM1	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.2090248211312524	[33]	[25]	-7	hSpCas9	negative selection	10725010
40798131	40798154	5	-	28162770	SKM1	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.8220016980220053	[7]	[7]	-6	hSpCas9	negative selection	10725011
40798088	40798111	5	-	28162770	SKM1	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.583214838434889	[99]	[58]	-8	hSpCas9	negative selection	10725012
40767485	40767508	5	-	28162770	SKM1	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.2854022188622485	[90]	[65]	-7	hSpCas9	negative selection	10725013
40777458	40777481	5	+	28162770	SKM1	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.28766527037081735	[28]	[41]	-2	hSpCas9	negative selection	10725014
40771788	40771811	5	-	28162770	SKM1	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.8220016980220056	[82]	[82]	-6	hSpCas9	negative selection	10725015
40767671	40767694	5	-	28162770	SKM1	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.9151111024134868	[127]	[119]	-6	hSpCas9	negative selection	10725016
40764912	40764935	5	+	28162770	SKM1	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.5356975128653644	[40]	[49]	-4	hSpCas9	negative selection	10725017
40777509	40777532	5	-	28162770	PL21	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-1.4471071644861786	[212]	[33]	-7	hSpCas9	negative selection	10894374
40764858	40764881	5	-	28162770	PL21	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.5436796507179487	[210]	[62]	-4	hSpCas9	negative selection	10894375
40767652	40767675	5	-	28162770	PL21	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-4.434066405251686	[148]	[2]	-9	hSpCas9	negative selection	10894376
40798131	40798154	5	-	28162770	PL21	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.38482288623183614	[35]	[11]	-3	hSpCas9	negative selection	10894377
40798088	40798111	5	-	28162770	PL21	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-3.4928826320892887	[387]	[14]	-9	hSpCas9	negative selection	10894378
40767485	40767508	5	-	28162770	PL21	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.2727962684977245	[321]	[57]	-7	hSpCas9	negative selection	10894379
40777458	40777481	5	+	28162770	PL21	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-2.066646926205581	[153]	[15]	-8	hSpCas9	negative selection	10894380
40771788	40771811	5	-	28162770	PL21	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.09414572532891341	[335]	[136]	0	hSpCas9	negative selection	10894381
40767671	40767694	5	-	28162770	PL21	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.4496740305818039	[454]	[144]	-3	hSpCas9	negative selection	10894382
40764912	40764935	5	+	28162770	PL21	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.8342681720143607	[124]	[96]	7	hSpCas9	negative selection	10894383
40777509	40777532	5	-	28162770	P31FUJ	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.794360100800591	[213]	[144]	-6	hSpCas9	negative selection	11063740
40764858	40764881	5	-	28162770	P31FUJ	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.3147134022009962	[307]	[290]	-3	hSpCas9	negative selection	11063741
40767652	40767675	5	-	28162770	P31FUJ	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.1274492042681499	[131]	[70]	-7	hSpCas9	negative selection	11063742
40798131	40798154	5	-	28162770	P31FUJ	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.0559244423302996	[22]	[12]	-6	hSpCas9	negative selection	11063743
40798088	40798111	5	-	28162770	P31FUJ	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.0272180707644845	[332]	[191]	-6	hSpCas9	negative selection	11063744
40767485	40767508	5	-	28162770	P31FUJ	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.0704199651893294	[276]	[154]	-6	hSpCas9	negative selection	11063745
40777458	40777481	5	+	28162770	P31FUJ	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.0749225446326593	[146]	[81]	-6	hSpCas9	negative selection	11063746
40771788	40771811	5	-	28162770	P31FUJ	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.509693410923571	[543]	[448]	-4	hSpCas9	negative selection	11063747
40767671	40767694	5	-	28162770	P31FUJ	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.4595730662614008	[604]	[516]	-4	hSpCas9	negative selection	11063748
40764912	40764935	5	+	28162770	P31FUJ	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.7174447603885217	[176]	[341]	7	hSpCas9	negative selection	11063749
40777509	40777532	5	-	28162770	OCIAML5	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	0.5691760779415549	[86]	[232]	5	hSpCas9	negative selection	11233106
40764858	40764881	5	-	28162770	OCIAML5	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.15980078416860494	[176]	[285]	-1	hSpCas9	negative selection	11233107
40767652	40767675	5	-	28162770	OCIAML5	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	0.42185615111253305	[54]	[132]	3	hSpCas9	negative selection	11233108
40798131	40798154	5	-	28162770	OCIAML5	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	1.1734685212431402	[13]	[56]	8	hSpCas9	negative selection	11233109
40798088	40798111	5	-	28162770	OCIAML5	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-0.1700366521826761	[181]	[291]	-1	hSpCas9	negative selection	11233110
40767485	40767508	5	-	28162770	OCIAML5	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.5644899807674117	[203]	[248]	-4	hSpCas9	negative selection	11233111
40777458	40777481	5	+	28162770	OCIAML5	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.023547038808350837	[115]	[205]	0	hSpCas9	negative selection	11233112
40771788	40771811	5	-	28162770	OCIAML5	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.3310567029320567	[245]	[352]	-2	hSpCas9	negative selection	11233113
40767671	40767694	5	-	28162770	OCIAML5	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.21103800772208192	[285]	[445]	-1	hSpCas9	negative selection	11233114
40764912	40764935	5	+	28162770	OCIAML5	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.34903107210922263	[116]	[268]	3	hSpCas9	negative selection	11233115
40777509	40777532	5	-	28162770	EOL1	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-1.2425622142647237	[511]	[113]	-7	hSpCas9	negative selection	11402472
40764858	40764881	5	-	28162770	EOL1	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	0.039586279836751115	[252]	[136]	0	hSpCas9	negative selection	11402473
40767652	40767675	5	-	28162770	EOL1	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.4620332816590813	[250]	[47]	-7	hSpCas9	negative selection	11402474
40798131	40798154	5	-	28162770	EOL1	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.9662231586747072	[88]	[11]	-8	hSpCas9	negative selection	11402475
40798088	40798111	5	-	28162770	EOL1	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-2.2277023375485867	[648]	[72]	-8	hSpCas9	negative selection	11402476
40767485	40767508	5	-	28162770	EOL1	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.1950954754289547	[577]	[132]	-7	hSpCas9	negative selection	11402477
40777458	40777481	5	+	28162770	EOL1	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.9014228286544166	[155]	[21]	-8	hSpCas9	negative selection	11402478
40771788	40771811	5	-	28162770	EOL1	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-1.4279686431502507	[428]	[83]	-7	hSpCas9	negative selection	11402479
40767671	40767694	5	-	28162770	EOL1	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.4853280225925304	[650]	[244]	-4	hSpCas9	negative selection	11402480
40764912	40764935	5	+	28162770	EOL1	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.40098581551352164	[137]	[95]	3	hSpCas9	negative selection	11402481
40777509	40777532	5	-	28162770	MOLM13	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.43287274551262167	[322]	[172]	-3	hSpCas9	negative selection	11571838
40764858	40764881	5	-	28162770	MOLM13	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-2.1855528570593443	[452]	[71]	-7	hSpCas9	negative selection	11571839
40767652	40767675	5	-	28162770	MOLM13	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.2399298669621108	[195]	[59]	-6	hSpCas9	negative selection	11571840
40798131	40798154	5	-	28162770	MOLM13	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.2841831050118357	[63]	[18]	-6	hSpCas9	negative selection	11571841
40798088	40798111	5	-	28162770	MOLM13	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	0.2583463824751423	[502]	[434]	1	hSpCas9	negative selection	11571842
40767485	40767508	5	-	28162770	MOLM13	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	0.09138475380240552	[430]	[331]	0	hSpCas9	negative selection	11571843
40777458	40777481	5	+	28162770	MOLM13	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.961422248716383	[236]	[43]	-7	hSpCas9	negative selection	11571844
40771788	40771811	5	-	28162770	MOLM13	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-1.3208498623456784	[538]	[155]	-6	hSpCas9	negative selection	11571845
40767671	40767694	5	-	28162770	MOLM13	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	0.2287777630511909	[621]	[526]	1	hSpCas9	negative selection	11571846
40764912	40764935	5	+	28162770	MOLM13	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.10209540128558409	[191]	[148]	0	hSpCas9	negative selection	11571847
40777509	40777532	5	-	28162770	HEL	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-0.23861667150102397	[314]	[170]	-2	hSpCas9	negative selection	11741204
40764858	40764881	5	-	28162770	HEL	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-3.250976374860428	[326]	[21]	-9	hSpCas9	negative selection	11741205
40767652	40767675	5	-	28162770	HEL	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	0.006429451278980203	[142]	[91]	0	hSpCas9	negative selection	11741206
40798131	40798154	5	-	28162770	HEL	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-0.568765273193355	[43]	[18]	-4	hSpCas9	negative selection	11741207
40798088	40798111	5	-	28162770	HEL	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-0.12553088208385876	[527]	[309]	-1	hSpCas9	negative selection	11741208
40767485	40767508	5	-	28162770	HEL	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-0.5577941553723516	[392]	[170]	-4	hSpCas9	negative selection	11741209
40777458	40777481	5	+	28162770	HEL	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-0.8706704076957652	[176]	[61]	-5	hSpCas9	negative selection	11741210
40771788	40771811	5	-	28162770	HEL	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-1.0821539298652743	[475]	[143]	-6	hSpCas9	negative selection	11741211
40767671	40767694	5	-	28162770	HEL	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.2618416029209606	[467]	[249]	-2	hSpCas9	negative selection	11741212
40764912	40764935	5	+	28162770	HEL	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.5365848674442051	[153]	[67]	-4	hSpCas9	negative selection	11741213
40777509	40777532	5	-	28162770	MV411	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-2.156676299459832	[306]	[57]	-8	hSpCas9	negative selection	11910570
40764858	40764881	5	-	28162770	MV411	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.2811116410331147	[162]	[112]	-2	hSpCas9	negative selection	11910571
40767652	40767675	5	-	28162770	MV411	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.8450086979818194	[144]	[33]	-8	hSpCas9	negative selection	11910572
40798131	40798154	5	-	28162770	MV411	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-3.3964186389919493	[24]	[1]	-9	hSpCas9	negative selection	11910573
40798088	40798111	5	-	28162770	MV411	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.0091273597214607	[355]	[148]	-6	hSpCas9	negative selection	11910574
40767485	40767508	5	-	28162770	MV411	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.1151325286019331	[305]	[118]	-6	hSpCas9	negative selection	11910575
40777458	40777481	5	+	28162770	MV411	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.3159917883887395	[132]	[44]	-7	hSpCas9	negative selection	11910576
40771788	40771811	5	-	28162770	MV411	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.01228412101018278	[260]	[217]	0	hSpCas9	negative selection	11910577
40767671	40767694	5	-	28162770	MV411	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.6181122494448439	[429]	[235]	-4	hSpCas9	negative selection	11910578
40764912	40764935	5	+	28162770	MV411	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.8838069824954774	[137]	[62]	-6	hSpCas9	negative selection	11910579
40777509	40777532	5	-	28162770	MONOMAC1	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-2.5853074367756124	[177]	[33]	-9	hSpCas9	negative selection	12079936
40764858	40764881	5	-	28162770	MONOMAC1	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	0.26150229085587684	[146]	[201]	2	hSpCas9	negative selection	12079937
40767652	40767675	5	-	28162770	MONOMAC1	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	1.1392465408048789	[79]	[201]	8	hSpCas9	negative selection	12079938
40798131	40798154	5	-	28162770	MONOMAC1	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-1.0595333233096187	[19]	[10]	-7	hSpCas9	negative selection	12079939
40798088	40798111	5	-	28162770	MONOMAC1	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-0.5423289350124265	[248]	[195]	-4	hSpCas9	negative selection	12079940
40767485	40767508	5	-	28162770	MONOMAC1	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.9415756609305608	[190]	[56]	-8	hSpCas9	negative selection	12079941
40777458	40777481	5	+	28162770	MONOMAC1	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.0783923505609345	[69]	[37]	-7	hSpCas9	negative selection	12079942
40771788	40771811	5	-	28162770	MONOMAC1	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.8734977022662059	[178]	[111]	-6	hSpCas9	negative selection	12079943
40767671	40767694	5	-	28162770	MONOMAC1	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.5127597924202079	[289]	[232]	-4	hSpCas9	negative selection	12079944
40764912	40764935	5	+	28162770	MONOMAC1	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	-0.6171453358858197	[94]	[70]	-5	hSpCas9	negative selection	12079945
40777509	40777532	5	-	28162770	OCIAML2	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-1.4706298252421612	[52]	[105]	-7	hSpCas9	negative selection	12249302
40764858	40764881	5	-	28162770	OCIAML2	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-0.49864420141175786	[25]	[101]	-4	hSpCas9	negative selection	12249303
40767652	40767675	5	-	28162770	OCIAML2	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-1.1976113308357452	[23]	[57]	-7	hSpCas9	negative selection	12249304
40798131	40798154	5	-	28162770	OCIAML2	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	0.6992951762001511	[2]	[26]	6	hSpCas9	negative selection	12249305
40798088	40798111	5	-	28162770	OCIAML2	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-1.0760979813979588	[61]	[162]	-6	hSpCas9	negative selection	12249306
40767485	40767508	5	-	28162770	OCIAML2	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.5567864689918758	[68]	[129]	-7	hSpCas9	negative selection	12249307
40777458	40777481	5	+	28162770	OCIAML2	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.5637392296336425	[31]	[59]	-7	hSpCas9	negative selection	12249308
40771788	40771811	5	-	28162770	OCIAML2	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.7412774151858302	[56]	[188]	-5	hSpCas9	negative selection	12249309
40767671	40767694	5	-	28162770	OCIAML2	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.6939065218382204	[67]	[232]	-5	hSpCas9	negative selection	12249310
40764912	40764935	5	+	28162770	OCIAML2	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.5293701747578389	[20]	[167]	5	hSpCas9	negative selection	12249311
40777509	40777532	5	-	28162770	OCIAML3	viability	GAAGATTCGGAGCCTTGATGTGG	PRKAA1	ENSG00000132356	-3.5482039507110383	[61]	[15]	-9	hSpCas9	negative selection	12418668
40764858	40764881	5	-	28162770	OCIAML3	viability	CCACAGAAATCCAAACACCAAGG	PRKAA1	ENSG00000132356	-2.3309732344903695	[59]	[35]	-9	hSpCas9	negative selection	12418669
40767652	40767675	5	-	28162770	OCIAML3	viability	GAGGTAGATATATGGAGCAGTGG	PRKAA1	ENSG00000132356	-3.1383281565479733	[34]	[11]	-9	hSpCas9	negative selection	12418670
40798131	40798154	5	-	28162770	OCIAML3	viability	GCCGAGAAGCAGAAACACGACGG	PRKAA1	ENSG00000132356	-3.5940076403241634	[7]	[1]	-9	hSpCas9	negative selection	12418671
40798088	40798111	5	-	28162770	OCIAML3	viability	ACATTCTGGGTGACACGCTGGGG	PRKAA1	ENSG00000132356	-2.6627203904081775	[85]	[40]	-9	hSpCas9	negative selection	12418672
40767485	40767508	5	-	28162770	OCIAML3	viability	GCAGGTGGATCCCATGAAGAGGG	PRKAA1	ENSG00000132356	-1.6471189767837258	[82]	[79]	-8	hSpCas9	negative selection	12418673
40777458	40777481	5	+	28162770	OCIAML3	viability	GAGGATGCCTGAAAAGCTTGAGG	PRKAA1	ENSG00000132356	-1.3478470530547657	[42]	[50]	-7	hSpCas9	negative selection	12418674
40771788	40771811	5	-	28162770	OCIAML3	viability	TTATTGTCACAGGCATATGGTGG	PRKAA1	ENSG00000132356	-0.6739759450275783	[101]	[192]	-5	hSpCas9	negative selection	12418675
40767671	40767694	5	-	28162770	OCIAML3	viability	CAGATTGTATGCAGGCCCAGAGG	PRKAA1	ENSG00000132356	-0.9613125288189054	[108]	[168]	-6	hSpCas9	negative selection	12418676
40764912	40764935	5	+	28162770	OCIAML3	viability	GGTGTTTCAGCAACCAAGAATGG	PRKAA1	ENSG00000132356	0.2804614775919778	[56]	[208]	2	hSpCas9	negative selection	12418677