Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:9037
• Symbol:SEMA5A
• Description:semaphorin 5A

DataSource: http://genomecrispr.dkfz.de/#!/results/SEMA5A

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
9044516	9044539	5	+	26472758	Jiyoye	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-1.5403571985999913	[80]	[20]	-7	hSpCas9	negative selection	144223
9226943	9226966	5	-	26472758	Jiyoye	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.1355789621686555	[168]	[139]	1	hSpCas9	negative selection	144224
9197267	9197290	5	+	26472758	Jiyoye	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	0.40186158160940555	[271]	[270]	3	hSpCas9	negative selection	144225
9136551	9136574	5	-	26472758	Jiyoye	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	1.0090117007914259	[84]	[128]	6	hSpCas9	negative selection	144226
9136528	9136551	5	-	26472758	Jiyoye	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	2.0471792457849913	[59]	[186]	8	hSpCas9	negative selection	144227
9108202	9108225	5	-	26472758	Jiyoye	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	-0.5556716200766604	[344]	[176]	-4	hSpCas9	negative selection	144228
9066552	9066575	5	-	26472758	Jiyoye	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.37802903584635694	[99]	[97]	3	hSpCas9	negative selection	144229
9054200	9054223	5	-	26472758	Jiyoye	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	1.1818830696636309	[82]	[141]	7	hSpCas9	negative selection	144230
9054139	9054162	5	+	26472758	Jiyoye	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	2.5540167698351444	[13]	[61]	9	hSpCas9	negative selection	144231
9044516	9044539	5	+	26472758	KBM7	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.6273395710882721	[177,204]	[77,41]	-5	hSpCas9	negative selection	335341
9226943	9226966	5	-	26472758	KBM7	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.4443997723775509	[591,679]	[500,331]	5	hSpCas9	negative selection	335342
9197267	9197290	5	+	26472758	KBM7	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.5377752724281707	[1542,1046]	[417,408]	-5	hSpCas9	negative selection	335343
9136551	9136574	5	-	26472758	KBM7	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	-0.00019873550423377476	[563,400]	[251,198]	0	hSpCas9	negative selection	335344
9136528	9136551	5	-	26472758	KBM7	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.3688493648031641	[626,550]	[441,280]	4	hSpCas9	negative selection	335345
9108202	9108225	5	-	26472758	KBM7	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.6114569921966295	[872,763]	[608,566]	6	hSpCas9	negative selection	335346
9066552	9066575	5	-	26472758	KBM7	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	-0.05242313869855042	[206,271]	[145,78]	0	hSpCas9	negative selection	335347
9054200	9054223	5	-	26472758	KBM7	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	1.395743905870643	[185,332]	[512,169]	9	hSpCas9	negative selection	335348
9054139	9054162	5	+	26472758	KBM7	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.37815976198715284	[218,88]	[80,98]	4	hSpCas9	negative selection	335349
9044516	9044539	5	+	26472758	Raji	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	0.3000286133159954	[118]	[33]	3	hSpCas9	negative selection	526459
9226943	9226966	5	-	26472758	Raji	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.658398704171418	[171]	[62]	6	hSpCas9	negative selection	526460
9197267	9197290	5	+	26472758	Raji	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.1594030053213017	[384]	[79]	-1	hSpCas9	negative selection	526461
9136551	9136574	5	-	26472758	Raji	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.9089699769715915	[178]	[77]	8	hSpCas9	negative selection	526462
9136528	9136551	5	-	26472758	Raji	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	-0.12899311539854963	[310]	[65]	-1	hSpCas9	negative selection	526463
9108202	9108225	5	-	26472758	Raji	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.5706301276950632	[411]	[141]	5	hSpCas9	negative selection	526464
9066552	9066575	5	-	26472758	Raji	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	-0.5312169289496299	[136]	[21]	-4	hSpCas9	negative selection	526465
9054200	9054223	5	-	26472758	Raji	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	1.1472762624971122	[106]	[54]	8	hSpCas9	negative selection	526466
9054139	9054162	5	+	26472758	Raji	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.8036027871964966	[78]	[31]	7	hSpCas9	negative selection	526467
9044516	9044539	5	+	26472758	K562	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-3.399437462108278	[224]	[17]	-8	hSpCas9	negative selection	717577
9226943	9226966	5	-	26472758	K562	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.5968522185533475	[364]	[465]	5	hSpCas9	negative selection	717578
9197267	9197290	5	+	26472758	K562	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-1.5111393276305851	[806]	[238]	-6	hSpCas9	negative selection	717579
9136551	9136574	5	-	26472758	K562	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.04738188060689327	[328]	[286]	0	hSpCas9	negative selection	717580
9136528	9136551	5	-	26472758	K562	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	1.1814238044354721	[432]	[828]	8	hSpCas9	negative selection	717581
9108202	9108225	5	-	26472758	K562	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.7494966424180615	[624]	[886]	6	hSpCas9	negative selection	717582
9066552	9066575	5	-	26472758	K562	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.2890088786905175	[222]	[229]	2	hSpCas9	negative selection	717583
9054200	9054223	5	-	26472758	K562	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	-0.0775093672209155	[229]	[183]	0	hSpCas9	negative selection	717584
9054139	9054162	5	+	26472758	K562	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.8776363969948241	[146]	[227]	7	hSpCas9	negative selection	717585
9197248	9197271	5	-	26627737	DLD1	viability	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-0.44674069604713207	[498]	[155,120,72]	-4	hSpCas9	negative selection	829736
9202112	9202135	5	+	26627737	DLD1	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.9093969334390102	[574]	[402,322,292]	8	hSpCas9	negative selection	829737
9224681	9224704	5	-	26627737	DLD1	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.4952598375962317	[787]	[416,335,295]	5	hSpCas9	negative selection	829738
9226920	9226943	5	-	26627737	DLD1	viability	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.32181524598385675	[581]	[143,354,193]	3	hSpCas9	negative selection	829739
9337782	9337805	5	+	26627737	DLD1	viability	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.67401103390658	[332]	[145,156,190]	6	hSpCas9	negative selection	829753
9197248	9197271	5	-	26627737	GBM cells	viability after 5 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.6700276914637375	[166]	[144,139]	8	hSpCas9	negative selection	912051
9202112	9202135	5	+	26627737	GBM cells	viability after 5 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.23517902070159719	[337]	[106,200]	-4	hSpCas9	negative selection	912052
9224681	9224704	5	-	26627737	GBM cells	viability after 5 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.42080958046963635	[451]	[343,304]	6	hSpCas9	negative selection	912053
9226920	9226943	5	-	26627737	GBM cells	viability after 5 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.7273024968154864	[213]	[180,198]	8	hSpCas9	negative selection	912054
9337782	9337805	5	+	26627737	GBM cells	viability after 5 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.14566062571454913	[192]	[139,88]	2	hSpCas9	negative selection	912068
9197248	9197271	5	-	26627737	GBM cells	viability after 13 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.0168784294794242	[166]	[200,168]	9	hSpCas9	negative selection	994366
9202112	9202135	5	+	26627737	GBM cells	viability after 13 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.22981120110399345	[337]	[192,232]	3	hSpCas9	negative selection	994367
9224681	9224704	5	-	26627737	GBM cells	viability after 13 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6742777441399392	[451]	[424,363]	7	hSpCas9	negative selection	994368
9226920	9226943	5	-	26627737	GBM cells	viability after 13 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.6870199587598127	[213]	[192,181]	8	hSpCas9	negative selection	994369
9337782	9337805	5	+	26627737	GBM cells	viability after 13 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.32654692480929626	[192]	[143,120]	4	hSpCas9	negative selection	994383
9197248	9197271	5	-	26627737	GBM cells	viability after 21 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.14726165916822004	[166]	[100,99]	1	hSpCas9	negative selection	1076681
9202112	9202135	5	+	26627737	GBM cells	viability after 21 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.11065219977737428	[337]	[191,203]	1	hSpCas9	negative selection	1076682
9224681	9224704	5	-	26627737	GBM cells	viability after 21 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6819840960754564	[451]	[429,360]	7	hSpCas9	negative selection	1076683
9226920	9226943	5	-	26627737	GBM cells	viability after 21 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.5387918311285347	[213]	[182,155]	5	hSpCas9	negative selection	1076684
9337782	9337805	5	+	26627737	GBM cells	viability after 21 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.22037064132274709	[192]	[89,89]	-2	hSpCas9	negative selection	1076698
9197248	9197271	5	-	26627737	RPE1	viability after 9 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-0.42344596185116046	[924]	[211,220]	-3	hSpCas9	negative selection	1158996
9202112	9202135	5	+	26627737	RPE1	viability after 9 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.3097320165148527	[1678]	[341,529]	-2	hSpCas9	negative selection	1158997
9224681	9224704	5	-	26627737	RPE1	viability after 9 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.806518003764674	[1551]	[813,895]	5	hSpCas9	negative selection	1158998
9226920	9226943	5	-	26627737	RPE1	viability after 9 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.37394169448058323	[1021]	[228,269]	-3	hSpCas9	negative selection	1158999
9337782	9337805	5	+	26627737	RPE1	viability after 9 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.14571117722286986	[550]	[182,120]	-1	hSpCas9	negative selection	1159013
9197248	9197271	5	-	26627737	RPE1	viability after 12 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-0.07176323081549338	[924]	[200,273]	0	hSpCas9	negative selection	1241311
9202112	9202135	5	+	26627737	RPE1	viability after 12 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.6967039502016146	[1678]	[220,338]	-5	hSpCas9	negative selection	1241312
9224681	9224704	5	-	26627737	RPE1	viability after 12 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.8983406601839925	[1551]	[732,823]	5	hSpCas9	negative selection	1241313
9226920	9226943	5	-	26627737	RPE1	viability after 12 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.23246595838033882	[1021]	[172,297]	-1	hSpCas9	negative selection	1241314
9337782	9337805	5	+	26627737	RPE1	viability after 12 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.4865506044387904	[550]	[161,45]	-3	hSpCas9	negative selection	1241328
9197248	9197271	5	-	26627737	RPE1	viability after 15 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-0.19177262462914532	[924]	[251,206]	-1	hSpCas9	negative selection	1323626
9202112	9202135	5	+	26627737	RPE1	viability after 15 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.6596346118590553	[1678]	[403,200]	-4	hSpCas9	negative selection	1323627
9224681	9224704	5	-	26627737	RPE1	viability after 15 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.7353180241388146	[1551]	[765,696]	4	hSpCas9	negative selection	1323628
9226920	9226943	5	-	26627737	RPE1	viability after 15 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.534040856435674	[1021]	[246,153]	-3	hSpCas9	negative selection	1323629
9337782	9337805	5	+	26627737	RPE1	viability after 15 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.5094620029636625	[550]	[137,81]	-3	hSpCas9	negative selection	1323643
9197248	9197271	5	-	26627737	RPE1	viability after 18 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.035156314027859376	[924]	[234,277]	0	hSpCas9	negative selection	1405941
9202112	9202135	5	+	26627737	RPE1	viability after 18 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.3189698547931842	[1678]	[369,355]	-2	hSpCas9	negative selection	1405942
9224681	9224704	5	-	26627737	RPE1	viability after 18 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6231333646359762	[1551]	[647,641]	4	hSpCas9	negative selection	1405943
9226920	9226943	5	-	26627737	RPE1	viability after 18 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.6336612291192532	[1021]	[272,75]	-4	hSpCas9	negative selection	1405944
9337782	9337805	5	+	26627737	RPE1	viability after 18 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.23999173267822516	[550]	[166,81]	-1	hSpCas9	negative selection	1405958
9197248	9197271	5	-	26627737	HeLa	viability after 8 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.31822641132534246	[222]	[150,118,100]	3	hSpCas9	negative selection	1488256
9202112	9202135	5	+	26627737	HeLa	viability after 8 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.8889024727994546	[375]	[419,275,242]	7	hSpCas9	negative selection	1488257
9224681	9224704	5	-	26627737	HeLa	viability after 8 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.16786345425040505	[738]	[356,337,385]	1	hSpCas9	negative selection	1488258
9226920	9226943	5	-	26627737	HeLa	viability after 8 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.3006930824578864	[327]	[148,218,154]	3	hSpCas9	negative selection	1488259
9337782	9337805	5	+	26627737	HeLa	viability after 8 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	1.490289699296354	[65]	[157,48,53]	9	hSpCas9	negative selection	1488273
9197248	9197271	5	-	26627737	HeLa	viability after 12 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.095405637921565	[222]	[202,82,316]	7	hSpCas9	negative selection	1570571
9202112	9202135	5	+	26627737	HeLa	viability after 12 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.9755066991703706	[375]	[443,273,205]	7	hSpCas9	negative selection	1570572
9224681	9224704	5	-	26627737	HeLa	viability after 12 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6777366168213889	[738]	[615,396,465]	5	hSpCas9	negative selection	1570573
9226920	9226943	5	-	26627737	HeLa	viability after 12 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.9627779781501644	[327]	[269,176,354]	7	hSpCas9	negative selection	1570574
9337782	9337805	5	+	26627737	HeLa	viability after 12 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.8709521285913264	[65]	[32,7,4]	-7	hSpCas9	negative selection	1570588
9197248	9197271	5	-	26627737	HeLa	viability after 15 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.4573848124950928	[222]	[366,170,204]	8	hSpCas9	negative selection	1652886
9202112	9202135	5	+	26627737	HeLa	viability after 15 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.41540387385448674	[375]	[260,142,207]	3	hSpCas9	negative selection	1652887
9224681	9224704	5	-	26627737	HeLa	viability after 15 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.7216503083245925	[738]	[290,546,665]	5	hSpCas9	negative selection	1652888
9226920	9226943	5	-	26627737	HeLa	viability after 15 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.9233017341546041	[327]	[186,169,415]	7	hSpCas9	negative selection	1652889
9337782	9337805	5	+	26627737	HeLa	viability after 15 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.4171768219404406	[65]	[31,35,39]	3	hSpCas9	negative selection	1652903
9197248	9197271	5	-	26627737	HeLa	viability after 18 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.454051741776452	[222]	[243,142,204]	8	hSpCas9	negative selection	1735201
9202112	9202135	5	+	26627737	HeLa	viability after 18 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.3888251889420828	[375]	[168,116,207]	3	hSpCas9	negative selection	1735202
9224681	9224704	5	-	26627737	HeLa	viability after 18 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.7341603199878789	[738]	[201,454,665]	6	hSpCas9	negative selection	1735203
9226920	9226943	5	-	26627737	HeLa	viability after 18 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.9136557243720609	[327]	[121,140,415]	7	hSpCas9	negative selection	1735204
9337782	9337805	5	+	26627737	HeLa	viability after 18 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.43937059976990067	[65]	[23,27,39]	3	hSpCas9	negative selection	1735218
9197248	9197271	5	-	26627737	HCT116	viability after 6 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-0.3670257067378301	[406]	[115,12]	-2	hSpCas9	negative selection	1817516
9202112	9202135	5	+	26627737	HCT116	viability after 6 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	1.0576454182002906	[870]	[168,348]	6	hSpCas9	negative selection	1817517
9224681	9224704	5	-	26627737	HCT116	viability after 6 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	-0.4750503622441622	[1061]	[239,53]	-3	hSpCas9	negative selection	1817518
9226920	9226943	5	-	26627737	HCT116	viability after 6 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	1.586289138239142	[640]	[241,335]	7	hSpCas9	negative selection	1817519
9337782	9337805	5	+	26627737	HCT116	viability after 6 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.8396518937494191	[569]	[22,64]	-5	hSpCas9	negative selection	1817533
9197248	9197271	5	-	26627737	HCT116	viability after 8 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.1660930717443747	[131]	[114,226,146]	8	hSpCas9	negative selection	1899831
9202112	9202135	5	+	26627737	HCT116	viability after 8 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.1525076926981317	[479]	[393,299,190]	1	hSpCas9	negative selection	1899832
9224681	9224704	5	-	26627737	HCT116	viability after 8 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6966922630665286	[489]	[401,454,454]	6	hSpCas9	negative selection	1899833
9226920	9226943	5	-	26627737	HCT116	viability after 8 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.9393657542454106	[225]	[67,291,351]	8	hSpCas9	negative selection	1899834
9337782	9337805	5	+	26627737	HCT116	viability after 8 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-1.0391679509440706	[423]	[69,132,136]	-8	hSpCas9	negative selection	1899848
9197248	9197271	5	-	26627737	HCT116	viability after 9 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	-1.1166636490092718	[406]	[48,50]	-7	hSpCas9	negative selection	1982146
9202112	9202135	5	+	26627737	HCT116	viability after 9 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.12963912955368767	[870]	[144,265]	-1	hSpCas9	negative selection	1982147
9224681	9224704	5	-	26627737	HCT116	viability after 9 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.5096033904576002	[1061]	[477,344]	4	hSpCas9	negative selection	1982148
9226920	9226943	5	-	26627737	HCT116	viability after 9 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.7647910490124277	[640]	[158,394]	5	hSpCas9	negative selection	1982149
9337782	9337805	5	+	26627737	HCT116	viability after 9 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.9286907613996603	[569]	[506,117]	6	hSpCas9	negative selection	1982163
9197248	9197271	5	-	26627737	HCT116	viability after 12 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.4153918512263977	[131]	[181,293,81]	9	hSpCas9	negative selection	2064461
9202112	9202135	5	+	26627737	HCT116	viability after 12 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.17822553508292105	[479]	[410,151,278]	1	hSpCas9	negative selection	2064462
9224681	9224704	5	-	26627737	HCT116	viability after 12 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.7306752246229395	[489]	[497,355,419]	6	hSpCas9	negative selection	2064463
9226920	9226943	5	-	26627737	HCT116	viability after 12 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	1.1346237035491873	[225]	[104,260,429]	8	hSpCas9	negative selection	2064464
9337782	9337805	5	+	26627737	HCT116	viability after 12 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.4505913100431308	[423]	[103,272,119]	-5	hSpCas9	negative selection	2064478
9197248	9197271	5	-	26627737	HCT116	viability after 15 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.7988040907734457	[131]	[179,413,187]	9	hSpCas9	negative selection	2146776
9202112	9202135	5	+	26627737	HCT116	viability after 15 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.1473654151607665	[479]	[338,198,219]	-1	hSpCas9	negative selection	2146777
9224681	9224704	5	-	26627737	HCT116	viability after 15 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.5310872513847826	[489]	[611,400,233]	5	hSpCas9	negative selection	2146778
9226920	9226943	5	-	26627737	HCT116	viability after 15 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	1.0979855526875248	[225]	[102,414,293]	8	hSpCas9	negative selection	2146779
9337782	9337805	5	+	26627737	HCT116	viability after 15 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.9716962414675427	[423]	[149,131,93]	-7	hSpCas9	negative selection	2146793
9197248	9197271	5	-	26627737	HCT116	viability after 18 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.8963336721568633	[131]	[225,350,122]	9	hSpCas9	negative selection	2229091
9202112	9202135	5	+	26627737	HCT116	viability after 18 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.13981006734150458	[479]	[381,143,247]	1	hSpCas9	negative selection	2229092
9224681	9224704	5	-	26627737	HCT116	viability after 18 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.6021219687528354	[489]	[287,523,250]	5	hSpCas9	negative selection	2229093
9226920	9226943	5	-	26627737	HCT116	viability after 18 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.5239510680087878	[225]	[100,139,234]	4	hSpCas9	negative selection	2229094
9337782	9337805	5	+	26627737	HCT116	viability after 18 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.9327993946234734	[423]	[38,187,88]	-7	hSpCas9	negative selection	2229108
9337768	9337793	5	-	24717434	HeLa	essential genes for diphteria toxin toxicity	GAGTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	14.663510819964376	[1911,1152,1526]	[46957,4,58]	9	hSpCas9	positive selection	2381352
9337768	9337793	5	-	24717434	HeLa	essential genes for PA/LFnDTA toxicity	GAGTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	7.482673473570995	[1911,1152,1526]	[69,39,213]	8	hSpCas9	positive selection	2382731
9337768	9337793	5	-	24717434	HeLa	essential genes for PA/LFnDTA toxicity	GAGTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	7.482673473570995	[1911,1152,1526]	[69,39,213]	8	hSpCas9	positive selection	2382732
9320690	9320713	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	AGGATCTCTCTCTGTCACCCAGG	SEMA5A	ENSG00000112902	0.0543668411750986	[86,177]	[47,10]	0	dCas9-VP64	positive selection	2412767
9131413	9131436	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	GGGGTTTCACCGTGTTAGCCTGG	SEMA5A	ENSG00000112902	0.853016361217498	[78,47]	[31,16]	3	dCas9-VP64	positive selection	2418402
9131395	9131418	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	TCAGGAGATCGAGACCATCCAGG	SEMA5A	ENSG00000112902	-0.5760906316419423	[119,210]	[24,22]	-3	dCas9-VP64	positive selection	2418481
9326736	9326759	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	CCCAGGCTGGAGTGCAGTGGCAG	SEMA5A	ENSG00000112902	-1.2921060931449064	[66,2]	[3,0]	-7	dCas9-VP64	positive selection	2435131
9064519	9064542	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	CATCCTCAGCAAACTAACACAAG	SEMA5A	ENSG00000112902	1.121085109230945	[120,28]	[47,21]	4	dCas9-VP64	positive selection	2435420
9540551	9540574	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	CGCGCCACTGCACCCCAGCCAGG	SEMA5A	ENSG00000112902	0.36632392651586576	[208,84]	[58,21]	1	dCas9-VP64	positive selection	2442350
9507886	9507909	5	+	25494202	A375	resistance to PLX-4720 (puromycin)	CCCAGCTACTCGGGAGGCTGAGG	SEMA5A	ENSG00000112902	-0.4892893999709095	[44,388]	[14,54]	-2	dCas9-VP64	positive selection	2465863
9080306	9080329	5	-	25494202	A375	resistance to PLX-4720 (puromycin)	GAGTGAGAACATGTGGTGTTAGG	SEMA5A	ENSG00000112902	-1.1334071955764224	[258,107]	[23,10]	-6	dCas9-VP64	positive selection	2468919
9320690	9320713	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	AGGATCTCTCTCTGTCACCCAGG	SEMA5A	ENSG00000112902	1.1651069513265528	[45,156]	[22,119]	7	dCas9-VP64	positive selection	2508022
9131413	9131436	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	GGGGTTTCACCGTGTTAGCCTGG	SEMA5A	ENSG00000112902	1.9224239682243596	[76,41]	[141,10]	8	dCas9-VP64	positive selection	2513657
9131395	9131418	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	TCAGGAGATCGAGACCATCCAGG	SEMA5A	ENSG00000112902	-0.5911896850137947	[183,271]	[71,26]	-6	dCas9-VP64	positive selection	2513736
9326736	9326759	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	CCCAGGCTGGAGTGCAGTGGCAG	SEMA5A	ENSG00000112902	0.4307409852930557	[33,0]	[13,0]	3	dCas9-VP64	positive selection	2530386
9064519	9064542	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	CATCCTCAGCAAACTAACACAAG	SEMA5A	ENSG00000112902	-0.7345820344479377	[224,163]	[25,48]	-7	dCas9-VP64	positive selection	2530675
9540551	9540574	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	CGCGCCACTGCACCCCAGCCAGG	SEMA5A	ENSG00000112902	-0.6605575882505516	[226,108]	[36,31]	-6	dCas9-VP64	positive selection	2537605
9507886	9507909	5	+	25494202	A375	resistance to PLX-4720 (zeocin)	CCCAGCTACTCGGGAGGCTGAGG	SEMA5A	ENSG00000112902	-0.16148281958930955	[134,276]	[52,65]	-1	dCas9-VP64	positive selection	2561118
9080306	9080329	5	-	25494202	A375	resistance to PLX-4720 (zeocin)	GAGTGAGAACATGTGGTGTTAGG	SEMA5A	ENSG00000112902	-0.007859909983208867	[140,86]	[47,26]	0	dCas9-VP64	positive selection	2564174
9202094	9202117	5	-	27260157	DLD1	viability	TATTGGTGGGCGCTTCCTGCTGG	SEMA5A	ENSG00000112902	0.152161689595207	[]	[]	3	hSpCas9	negative selection	2574749
9224720	9224743	5	+	27260157	DLD1	viability	AATGCCTAGGCTTCGGTAAATGG	SEMA5A	ENSG00000112902	0.6047878273508	[]	[]	8	hSpCas9	negative selection	2578466
9224857	9224880	5	+	27260157	DLD1	viability	TCTGATCATGGATCTCAGTCAGG	SEMA5A	ENSG00000112902	0.422035783370048	[]	[]	7	hSpCas9	negative selection	2580069
9226905	9226928	5	-	27260157	DLD1	viability	GCGACCGGTTATTCACCTGTGGG	SEMA5A	ENSG00000112902	0.497003779067616	[]	[]	8	hSpCas9	negative selection	2582118
9224788	9224811	5	-	27260157	DLD1	viability	AGCGCTCCTCACAGCTGGTGGGG	SEMA5A	ENSG00000112902	-0.886810477333381	[]	[]	-6	hSpCas9	negative selection	2582279
9337768	9337791	5	-	27260157	DLD1	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.52694843159224	[]	[]	8	hSpCas9	negative selection	2584950
9226901	9226924	5	+	27260157	DLD1	viability	TGGTCCCACAGGTGAATAACCGG	SEMA5A	ENSG00000112902	0.221099460588575	[]	[]	3	hSpCas9	negative selection	2605040
9226930	9226953	5	-	27260157	DLD1	viability	TACATCCGGGTGCTTCTGGTGGG	SEMA5A	ENSG00000112902	-0.0819217774781363	[]	[]	0	hSpCas9	negative selection	2607680
9224681	9224704	5	-	27260157	DLD1	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.737666172161274	[]	[]	8	hSpCas9	negative selection	2608782
9337781	9337804	5	+	27260157	DLD1	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.321059194148533	[]	[]	-3	hSpCas9	negative selection	2613103
9224845	9224868	5	-	27260157	DLD1	viability	CCATGATCAGATCAGTGGCATGG	SEMA5A	ENSG00000112902	0.569576916963787	[]	[]	8	hSpCas9	negative selection	2614067
9224764	9224787	5	-	27260157	DLD1	viability	GCTCTATGCTGCTACAGCCATGG	SEMA5A	ENSG00000112902	1.01362208853547	[]	[]	9	hSpCas9	negative selection	2614113
9226867	9226890	5	+	27260157	DLD1	viability	ACCGAGCGGTTGGTGCAGACAGG	SEMA5A	ENSG00000112902	0.242636349072509	[]	[]	4	hSpCas9	negative selection	2617109
9202108	9202131	5	-	27260157	DLD1	viability	GTGTGCAAGAACGATATTGGTGG	SEMA5A	ENSG00000112902	-0.19481614828647	[]	[]	-1	hSpCas9	negative selection	2619370
9224736	9224759	5	+	27260157	DLD1	viability	TAAATGGCAGGATCACGTCCTGG	SEMA5A	ENSG00000112902	0.479649491338972	[]	[]	8	hSpCas9	negative selection	2621161
9201973	9201996	5	-	27260157	DLD1	viability	CCTGAGCTGGATTTGATCTATGG	SEMA5A	ENSG00000112902	0.754797029743108	[]	[]	8	hSpCas9	negative selection	2623849
9202112	9202135	5	+	27260157	DLD1	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.199296697661448	[]	[]	3	hSpCas9	negative selection	2642496
9224803	9224826	5	+	27260157	DLD1	viability	GGAGCGCTGTGGAATTGTGCTGG	SEMA5A	ENSG00000112902	0.861445961920944	[]	[]	9	hSpCas9	negative selection	2646704
9224686	9224709	5	+	27260157	DLD1	viability	TGGAGTTGTACTGCGCCGTGCGG	SEMA5A	ENSG00000112902	-2.49898880443465	[]	[]	-9	hSpCas9	negative selection	2646985
9202094	9202117	5	-	27260157	HT1080	viability	TATTGGTGGGCGCTTCCTGCTGG	SEMA5A	ENSG00000112902	-0.323367789323943	[]	[]	-7	hSpCas9	negative selection	2650879
9224720	9224743	5	+	27260157	HT1080	viability	AATGCCTAGGCTTCGGTAAATGG	SEMA5A	ENSG00000112902	-0.25827732981891	[]	[]	0	hSpCas9	negative selection	2654596
9224857	9224880	5	+	27260157	HT1080	viability	TCTGATCATGGATCTCAGTCAGG	SEMA5A	ENSG00000112902	0.145790840215994	[]	[]	1	hSpCas9	negative selection	2656199
9226905	9226928	5	-	27260157	HT1080	viability	GCGACCGGTTATTCACCTGTGGG	SEMA5A	ENSG00000112902	-1.10322461979298	[]	[]	-8	hSpCas9	negative selection	2658248
9224788	9224811	5	-	27260157	HT1080	viability	AGCGCTCCTCACAGCTGGTGGGG	SEMA5A	ENSG00000112902	-1.73802546879499	[]	[]	-9	hSpCas9	negative selection	2658409
9337768	9337791	5	-	27260157	HT1080	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.241280564371838	[]	[]	0	hSpCas9	negative selection	2661080
9226901	9226924	5	+	27260157	HT1080	viability	TGGTCCCACAGGTGAATAACCGG	SEMA5A	ENSG00000112902	-0.95344013003103	[]	[]	-8	hSpCas9	negative selection	2681170
9226930	9226953	5	-	27260157	HT1080	viability	TACATCCGGGTGCTTCTGGTGGG	SEMA5A	ENSG00000112902	0.088617769772997	[]	[]	1	hSpCas9	negative selection	2683810
9224681	9224704	5	-	27260157	HT1080	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.528079167969802	[]	[]	4	hSpCas9	negative selection	2684912
9337781	9337804	5	+	27260157	HT1080	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-1.65012601470185	[]	[]	-9	hSpCas9	negative selection	2689233
9224845	9224868	5	-	27260157	HT1080	viability	CCATGATCAGATCAGTGGCATGG	SEMA5A	ENSG00000112902	1.01887411135544	[]	[]	8	hSpCas9	negative selection	2690197
9224764	9224787	5	-	27260157	HT1080	viability	GCTCTATGCTGCTACAGCCATGG	SEMA5A	ENSG00000112902	0.472913686421938	[]	[]	4	hSpCas9	negative selection	2690243
9226867	9226890	5	+	27260157	HT1080	viability	ACCGAGCGGTTGGTGCAGACAGG	SEMA5A	ENSG00000112902	-0.0206904580819292	[]	[]	0	hSpCas9	negative selection	2693239
9202108	9202131	5	-	27260157	HT1080	viability	GTGTGCAAGAACGATATTGGTGG	SEMA5A	ENSG00000112902	0.123767996496547	[]	[]	1	hSpCas9	negative selection	2695500
9224736	9224759	5	+	27260157	HT1080	viability	TAAATGGCAGGATCACGTCCTGG	SEMA5A	ENSG00000112902	0.187216368852735	[]	[]	1	hSpCas9	negative selection	2697291
9201973	9201996	5	-	27260157	HT1080	viability	CCTGAGCTGGATTTGATCTATGG	SEMA5A	ENSG00000112902	0.34429945114527	[]	[]	3	hSpCas9	negative selection	2699979
9202112	9202135	5	+	27260157	HT1080	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.386232347945767	[]	[]	3	hSpCas9	negative selection	2718626
9224803	9224826	5	+	27260157	HT1080	viability	GGAGCGCTGTGGAATTGTGCTGG	SEMA5A	ENSG00000112902	0.458211765823136	[]	[]	4	hSpCas9	negative selection	2722834
9224686	9224709	5	+	27260157	HT1080	viability	TGGAGTTGTACTGCGCCGTGCGG	SEMA5A	ENSG00000112902	-2.16305960542378	[]	[]	-9	hSpCas9	negative selection	2723115
9202094	9202117	5	-	27260157	MKN45	viability	TATTGGTGGGCGCTTCCTGCTGG	SEMA5A	ENSG00000112902	0.330701612442968	[]	[]	6	hSpCas9	negative selection	2727009
9224720	9224743	5	+	27260157	MKN45	viability	AATGCCTAGGCTTCGGTAAATGG	SEMA5A	ENSG00000112902	0.112440155585007	[]	[]	4	hSpCas9	negative selection	2730726
9224857	9224880	5	+	27260157	MKN45	viability	TCTGATCATGGATCTCAGTCAGG	SEMA5A	ENSG00000112902	1.30504545062703	[]	[]	9	hSpCas9	negative selection	2732329
9226905	9226928	5	-	27260157	MKN45	viability	GCGACCGGTTATTCACCTGTGGG	SEMA5A	ENSG00000112902	-0.108773167895828	[]	[]	0	hSpCas9	negative selection	2734378
9224788	9224811	5	-	27260157	MKN45	viability	AGCGCTCCTCACAGCTGGTGGGG	SEMA5A	ENSG00000112902	-1.11227182147384	[]	[]	-8	hSpCas9	negative selection	2734539
9337768	9337791	5	-	27260157	MKN45	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	1.15069360330053	[]	[]	8	hSpCas9	negative selection	2737210
9226901	9226924	5	+	27260157	MKN45	viability	TGGTCCCACAGGTGAATAACCGG	SEMA5A	ENSG00000112902	0.239603042273804	[]	[]	5	hSpCas9	negative selection	2757300
9226930	9226953	5	-	27260157	MKN45	viability	TACATCCGGGTGCTTCTGGTGGG	SEMA5A	ENSG00000112902	-0.0775865837942683	[]	[]	0	hSpCas9	negative selection	2759940
9224681	9224704	5	-	27260157	MKN45	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.578892439902222	[]	[]	7	hSpCas9	negative selection	2761042
9337781	9337804	5	+	27260157	MKN45	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-1.53299872407805	[]	[]	-9	hSpCas9	negative selection	2765363
9224845	9224868	5	-	27260157	MKN45	viability	CCATGATCAGATCAGTGGCATGG	SEMA5A	ENSG00000112902	1.23478022451356	[]	[]	9	hSpCas9	negative selection	2766327
9224764	9224787	5	-	27260157	MKN45	viability	GCTCTATGCTGCTACAGCCATGG	SEMA5A	ENSG00000112902	0.707015508260312	[]	[]	7	hSpCas9	negative selection	2766373
9226867	9226890	5	+	27260157	MKN45	viability	ACCGAGCGGTTGGTGCAGACAGG	SEMA5A	ENSG00000112902	0.690434076244388	[]	[]	7	hSpCas9	negative selection	2769369
9202108	9202131	5	-	27260157	MKN45	viability	GTGTGCAAGAACGATATTGGTGG	SEMA5A	ENSG00000112902	0.0381091134755679	[]	[]	0	hSpCas9	negative selection	2771630
9224736	9224759	5	+	27260157	MKN45	viability	TAAATGGCAGGATCACGTCCTGG	SEMA5A	ENSG00000112902	0.37660006010734	[]	[]	6	hSpCas9	negative selection	2773421
9201973	9201996	5	-	27260157	MKN45	viability	CCTGAGCTGGATTTGATCTATGG	SEMA5A	ENSG00000112902	-0.00203645254762444	[]	[]	0	hSpCas9	negative selection	2776109
9202112	9202135	5	+	27260157	MKN45	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.296069007974509	[]	[]	5	hSpCas9	negative selection	2794756
9224803	9224826	5	+	27260157	MKN45	viability	GGAGCGCTGTGGAATTGTGCTGG	SEMA5A	ENSG00000112902	1.60876073719178	[]	[]	9	hSpCas9	negative selection	2798964
9224686	9224709	5	+	27260157	MKN45	viability	TGGAGTTGTACTGCGCCGTGCGG	SEMA5A	ENSG00000112902	-1.75254798425675	[]	[]	-9	hSpCas9	negative selection	2799245
9202094	9202117	5	-	27260157	RKO	viability	TATTGGTGGGCGCTTCCTGCTGG	SEMA5A	ENSG00000112902	0.145711664335139	[]	[]	0	hSpCas9	negative selection	2803139
9224720	9224743	5	+	27260157	RKO	viability	AATGCCTAGGCTTCGGTAAATGG	SEMA5A	ENSG00000112902	-0.0717052518110139	[]	[]	0	hSpCas9	negative selection	2806856
9224857	9224880	5	+	27260157	RKO	viability	TCTGATCATGGATCTCAGTCAGG	SEMA5A	ENSG00000112902	0.911119279968237	[]	[]	8	hSpCas9	negative selection	2808459
9226905	9226928	5	-	27260157	RKO	viability	GCGACCGGTTATTCACCTGTGGG	SEMA5A	ENSG00000112902	-0.126593948274484	[]	[]	0	hSpCas9	negative selection	2810508
9224788	9224811	5	-	27260157	RKO	viability	AGCGCTCCTCACAGCTGGTGGGG	SEMA5A	ENSG00000112902	-0.812534269358146	[]	[]	-7	hSpCas9	negative selection	2810669
9337768	9337791	5	-	27260157	RKO	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	1.05075080508853	[]	[]	9	hSpCas9	negative selection	2813340
9226901	9226924	5	+	27260157	RKO	viability	TGGTCCCACAGGTGAATAACCGG	SEMA5A	ENSG00000112902	-0.0342389114295289	[]	[]	0	hSpCas9	negative selection	2833430
9226930	9226953	5	-	27260157	RKO	viability	TACATCCGGGTGCTTCTGGTGGG	SEMA5A	ENSG00000112902	-0.0337198498542741	[]	[]	0	hSpCas9	negative selection	2836070
9224681	9224704	5	-	27260157	RKO	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.00122902901290886	[]	[]	0	hSpCas9	negative selection	2837172
9337781	9337804	5	+	27260157	RKO	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-1.03493165102245	[]	[]	-8	hSpCas9	negative selection	2841493
9224845	9224868	5	-	27260157	RKO	viability	CCATGATCAGATCAGTGGCATGG	SEMA5A	ENSG00000112902	1.21403177381627	[]	[]	9	hSpCas9	negative selection	2842457
9224764	9224787	5	-	27260157	RKO	viability	GCTCTATGCTGCTACAGCCATGG	SEMA5A	ENSG00000112902	0.231023741010641	[]	[]	1	hSpCas9	negative selection	2842503
9226867	9226890	5	+	27260157	RKO	viability	ACCGAGCGGTTGGTGCAGACAGG	SEMA5A	ENSG00000112902	0.461043261293971	[]	[]	2	hSpCas9	negative selection	2845499
9202108	9202131	5	-	27260157	RKO	viability	GTGTGCAAGAACGATATTGGTGG	SEMA5A	ENSG00000112902	-0.327059925211128	[]	[]	-6	hSpCas9	negative selection	2847760
9224736	9224759	5	+	27260157	RKO	viability	TAAATGGCAGGATCACGTCCTGG	SEMA5A	ENSG00000112902	0.26184878741508	[]	[]	1	hSpCas9	negative selection	2849551
9201973	9201996	5	-	27260157	RKO	viability	CCTGAGCTGGATTTGATCTATGG	SEMA5A	ENSG00000112902	0.83760610907879	[]	[]	8	hSpCas9	negative selection	2852239
9202112	9202135	5	+	27260157	RKO	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.0282747696043319	[]	[]	0	hSpCas9	negative selection	2870886
9224803	9224826	5	+	27260157	RKO	viability	GGAGCGCTGTGGAATTGTGCTGG	SEMA5A	ENSG00000112902	-0.882322281899799	[]	[]	-8	hSpCas9	negative selection	2875094
9224686	9224709	5	+	27260157	RKO	viability	TGGAGTTGTACTGCGCCGTGCGG	SEMA5A	ENSG00000112902	-1.2304911351471	[]	[]	-8	hSpCas9	negative selection	2875375
9202094	9202117	5	-	27260157	SF268	viability	TATTGGTGGGCGCTTCCTGCTGG	SEMA5A	ENSG00000112902	-0.0188438146805756	[]	[]	0	hSpCas9	negative selection	2879269
9224720	9224743	5	+	27260157	SF268	viability	AATGCCTAGGCTTCGGTAAATGG	SEMA5A	ENSG00000112902	-0.25137880514734	[]	[]	-2	hSpCas9	negative selection	2882986
9224857	9224880	5	+	27260157	SF268	viability	TCTGATCATGGATCTCAGTCAGG	SEMA5A	ENSG00000112902	0.36907575281617	[]	[]	4	hSpCas9	negative selection	2884589
9226905	9226928	5	-	27260157	SF268	viability	GCGACCGGTTATTCACCTGTGGG	SEMA5A	ENSG00000112902	0.205938393068331	[]	[]	3	hSpCas9	negative selection	2886638
9224788	9224811	5	-	27260157	SF268	viability	AGCGCTCCTCACAGCTGGTGGGG	SEMA5A	ENSG00000112902	-1.31653387800499	[]	[]	-7	hSpCas9	negative selection	2886799
9337768	9337791	5	-	27260157	SF268	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.955919189061829	[]	[]	9	hSpCas9	negative selection	2889470
9226901	9226924	5	+	27260157	SF268	viability	TGGTCCCACAGGTGAATAACCGG	SEMA5A	ENSG00000112902	-0.402366038252358	[]	[]	-3	hSpCas9	negative selection	2909560
9226930	9226953	5	-	27260157	SF268	viability	TACATCCGGGTGCTTCTGGTGGG	SEMA5A	ENSG00000112902	0.71427234330361	[]	[]	8	hSpCas9	negative selection	2912200
9224681	9224704	5	-	27260157	SF268	viability	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	1.04575961307149	[]	[]	9	hSpCas9	negative selection	2913302
9337781	9337804	5	+	27260157	SF268	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.350266097064237	[]	[]	-3	hSpCas9	negative selection	2917623
9224845	9224868	5	-	27260157	SF268	viability	CCATGATCAGATCAGTGGCATGG	SEMA5A	ENSG00000112902	0.739882783636344	[]	[]	8	hSpCas9	negative selection	2918587
9224764	9224787	5	-	27260157	SF268	viability	GCTCTATGCTGCTACAGCCATGG	SEMA5A	ENSG00000112902	0.00875691117723799	[]	[]	0	hSpCas9	negative selection	2918633
9226867	9226890	5	+	27260157	SF268	viability	ACCGAGCGGTTGGTGCAGACAGG	SEMA5A	ENSG00000112902	0.331195843246327	[]	[]	4	hSpCas9	negative selection	2921629
9202108	9202131	5	-	27260157	SF268	viability	GTGTGCAAGAACGATATTGGTGG	SEMA5A	ENSG00000112902	-0.073563854243662	[]	[]	0	hSpCas9	negative selection	2923890
9224736	9224759	5	+	27260157	SF268	viability	TAAATGGCAGGATCACGTCCTGG	SEMA5A	ENSG00000112902	0.756241352985912	[]	[]	8	hSpCas9	negative selection	2925681
9201973	9201996	5	-	27260157	SF268	viability	CCTGAGCTGGATTTGATCTATGG	SEMA5A	ENSG00000112902	-0.165608788913976	[]	[]	-1	hSpCas9	negative selection	2928369
9202112	9202135	5	+	27260157	SF268	viability	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-0.240034571305478	[]	[]	-2	hSpCas9	negative selection	2947016
9224803	9224826	5	+	27260157	SF268	viability	GGAGCGCTGTGGAATTGTGCTGG	SEMA5A	ENSG00000112902	-0.0685419481869788	[]	[]	0	hSpCas9	negative selection	2951224
9224686	9224709	5	+	27260157	SF268	viability	TGGAGTTGTACTGCGCCGTGCGG	SEMA5A	ENSG00000112902	-1.60908256185174	[]	[]	-8	hSpCas9	negative selection	2951505
9226854	9226877	5	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	0.3733252384907142	[5,5]	[5,5]	7	hSpCas9	positive selection	3019403
9190342	9190365	5	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	-0.17405480780764748	[4,3]	[4,1]	-2	hSpCas9	positive selection	3019404
9190310	9190333	5	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	-0.19855157306806048	[2,1]	[2,0]	-3	hSpCas9	positive selection	3019405
9226868	9226891	5	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	-0.18743756524467864	[4,3]	[4,1]	-3	hSpCas9	positive selection	3019406
9226854	9226877	5	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	-0.2669864892897118	[5,5]	[4,1]	-4	hSpCas9	positive selection	3087261
9190342	9190365	5	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	-0.1937435542007101	[4,4]	[2,1]	-3	hSpCas9	positive selection	3087262
9190310	9190333	5	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	-0.6301980075530795	[2,2]	[1,0]	-8	hSpCas9	positive selection	3087263
9226868	9226891	5	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	-0.3828271660702608	[4,4]	[2,1]	-5	hSpCas9	positive selection	3087264
9337781	9337804		+	24336571	A375	resistance to PLX after 7 days	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.06948393923668725	[9,11]	[8,10]	-2	hSpCas9	positive selection	3143344
9337768	9337791		-	24336571	A375	resistance to PLX after 7 days	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.06967481611988977	[10,10]	[10,9]	-2	hSpCas9	positive selection	3143345
9337781	9337804		+	24336571	A375	resistance to PLX after 14 days	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.1526189235804159	[10,10]	[4,5]	1	hSpCas9	positive selection	3201137
9337768	9337791		-	24336571	A375	resistance to PLX after 14 days	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.5044964743235493	[12,7]	[2,2]	-6	hSpCas9	positive selection	3201138
9337781	9337804		+	24336571	A375	viability after 7 days	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.09029183043791619	[10]	[9,11]	1	hSpCas9	negative selection	3258930
9337768	9337791		-	24336571	A375	viability after 7 days	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.6259667301289725	[6]	[10,10]	8	hSpCas9	negative selection	3258931
9337781	9337804		+	24336571	A375	viability after 14 days	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.09289256241123295	[10]	[10,10]	1	hSpCas9	negative selection	3316723
9337768	9337791		-	24336571	A375	viability after 14 days	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.5706329540570722	[6]	[12,7]	7	hSpCas9	negative selection	3316724
9337768	9337791	5	-	27383988	293T	resistance to West Nile virus (flavivirus)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	2.00939869800225	[37,37]	[0,0]	7	hSpCas9	positive selection	3391766
9318389	9318412	5	+	27383988	293T	resistance to West Nile virus (flavivirus)	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.48914188666890873	[108,108]	[0,0]	2	hSpCas9	positive selection	3391767
9318402	9318425	5	-	27383988	293T	resistance to West Nile virus (flavivirus)	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	-1.7055697938914256	[498,498]	[0,0]	-9	hSpCas9	positive selection	3391768
9226853	9226876	5	+	26780180	HT29	viability (Avana library 4 designs)	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	-0.649842814853776	[]	[]	-8	hSpCas9	negative selection	3457851
9190341	9190364	5	+	26780180	HT29	viability (Avana library 4 designs)	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	-0.0045002523244328	[]	[]	0	hSpCas9	negative selection	3457852
9190309	9190332	5	+	26780180	HT29	viability (Avana library 4 designs)	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.462560418781652	[]	[]	1	hSpCas9	negative selection	3457853
9226868	9226891	5	-	26780180	HT29	viability (Avana library 4 designs)	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	-0.444291188038434	[]	[]	-6	hSpCas9	negative selection	3457854
9190309	9190332	5	+	26780180	A375	viability (Avana lentiCRISPRv2)	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	5.75327645516182	[]	[]	9	hSpCas9	negative selection	3557000
9190341	9190364	5	+	26780180	A375	viability (Avana lentiCRISPRv2)	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	2.30287368543978	[]	[]	3	hSpCas9	negative selection	3557001
9226853	9226876	5	+	26780180	A375	viability (Avana lentiCRISPRv2)	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	2.85655216233747	[]	[]	4	hSpCas9	negative selection	3557002
9226868	9226891	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	4.69731450735328	[]	[]	7	hSpCas9	negative selection	3557003
9337768	9337791	5	-	26780180	A375	viability (Avana lentiCRISPRv2)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	1.99901234210555	[]	[]	3	hSpCas9	negative selection	3557004
9190309	9190332	5	+	26780180	A375	viability (Avana lentiGuide)	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	-7.76637527474555	[]	[]	-9	hSpCas9	negative selection	3665659
9190341	9190364	5	+	26780180	A375	viability (Avana lentiGuide)	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	0.19530100546587	[]	[]	0	hSpCas9	negative selection	3665660
9226853	9226876	5	+	26780180	A375	viability (Avana lentiGuide)	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	-0.918906529521593	[]	[]	-1	hSpCas9	negative selection	3665661
9226868	9226891	5	-	26780180	A375	viability (Avana lentiGuide)	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	-3.40150219210982	[]	[]	-6	hSpCas9	negative selection	3665662
9337768	9337791	5	-	26780180	A375	viability (Avana lentiGuide)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-4.08172701759989	[]	[]	-7	hSpCas9	negative selection	3665663
9190309	9190332	5	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.027028109887508478	[3]	[3,2]	0	hSpCas9	positive selection	3774266
9190341	9190364	5	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	0.162954919694939	[5]	[6,5]	4	hSpCas9	positive selection	3774267
9226853	9226876	5	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	0.04487774546940537	[6]	[6,5]	1	hSpCas9	positive selection	3774268
9226868	9226891	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	0.27710242697954857	[4]	[5,5]	7	hSpCas9	positive selection	3774269
9337768	9337791	5	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.23817879857860424	[4]	[5,5]	6	hSpCas9	positive selection	3774270
9190309	9190332	5	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.20971655068495754	[3]	[3,3]	5	hSpCas9	positive selection	3882873
9190341	9190364	5	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CTATTGTCCTCCATGAAGGAGGG	SEMA5A	ENSG00000112902	-0.3418710265102404	[5]	[3,3]	-4	hSpCas9	positive selection	3882874
9226853	9226876	5	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CAAAAAGAAGCCATACCGAGCGG	SEMA5A	ENSG00000112902	0.2395619558172401	[6]	[7,6]	6	hSpCas9	positive selection	3882875
9226868	9226891	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GCCTGTCTGCACCAACCGCTCGG	SEMA5A	ENSG00000112902	0.2060994494870036	[4]	[5,4]	5	hSpCas9	positive selection	3882876
9337768	9337791	5	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.26242081449974153	[5]	[5,4]	7	hSpCas9	positive selection	3882877
9318389	9318412	5	+	26780180	A375	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	1.19816636007425	[]	[]	6	hSpCas9	negative selection	3992919
9337781	9337804	5	+	26780180	A375	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	1.39632084075315	[]	[]	7	hSpCas9	negative selection	3992920
9337768	9337791	5	-	26780180	A375	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.51869048851765	[]	[]	1	hSpCas9	negative selection	3992921
9318402	9318425	5	-	26780180	A375	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	-0.0821692687131549	[]	[]	-3	hSpCas9	negative selection	3992922
9318389	9318412	5	+	26780180	HT29	viability (GeCKOv2 library)	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.771453484206882	[]	[]	3	hSpCas9	negative selection	4100885
9337781	9337804	5	+	26780180	HT29	viability (GeCKOv2 library)	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	1.93007685292189	[]	[]	9	hSpCas9	negative selection	4100886
9337768	9337791	5	-	26780180	HT29	viability (GeCKOv2 library)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.450853592352147	[]	[]	1	hSpCas9	negative selection	4100887
9318402	9318425	5	-	26780180	HT29	viability (GeCKOv2 library)	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.459681232318607	[]	[]	1	hSpCas9	negative selection	4100888
9337781	9337804	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.02281324194509427	[3]	[2,2]	0	hSpCas9	positive selection	4169160
9337768	9337791	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.3925590324006156	[2]	[0,1]	-7	hSpCas9	positive selection	4169161
9337781	9337804	5	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.3139097827496138	[2]	[0,2,0,0]	-5	hSpCas9	positive selection	4233182
9337768	9337791	5	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	1.5804764258125856	[2]	[3,1,0,5]	9	hSpCas9	positive selection	4233183
9337781	9337804	5	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.012669643532257663	[2]	[2,1]	0	hSpCas9	positive selection	4296988
9337768	9337791	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.22281525224872345	[0]	[0,0]	4	hSpCas9	positive selection	4361242
9318389	9318412	5	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.378978772164041	[2]	[1,0]	-7	hSpCas9	positive selection	4361243
9318402	9318425	5	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.18343457573783956	[4]	[4,3]	3	hSpCas9	positive selection	4361244
9154483	9154505	5	-	27760321	OCIAML3	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	-0.17786611002931468	[652,581]	[401,457]	-2	hSpCas9	negative selection	4437784
9190310	9190332	5	+	27760321	OCIAML3	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.6568565664480966	[933,663]	[844,1143]	7	hSpCas9	negative selection	4437785
9190338	9190360	5	+	27760321	OCIAML3	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	-0.5306616220894022	[643,534]	[312,323]	-5	hSpCas9	negative selection	4437786
9197206	9197228	5	-	27760321	OCIAML3	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.89118066384689	[452,387]	[115,255]	-7	hSpCas9	negative selection	4437787
9226858	9226880	5	+	27760321	OCIAML3	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	-0.30350415931552793	[465,407]	[238,324]	-3	hSpCas9	negative selection	4437788
9154483	9154505	5	-	27760321	OCIAML2	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.5394684650526966	[652,581]	[620,861]	7	hSpCas9	negative selection	4521247
9190310	9190332	5	+	27760321	OCIAML2	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.613984671659349	[933,663]	[907,1071]	8	hSpCas9	negative selection	4521248
9190338	9190360	5	+	27760321	OCIAML2	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	-0.3554775101433353	[643,534]	[330,423]	-4	hSpCas9	negative selection	4521249
9197206	9197228	5	-	27760321	OCIAML2	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.30695432854661076	[452,387]	[233,326]	-4	hSpCas9	negative selection	4521250
9226858	9226880	5	+	27760321	OCIAML2	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.5891893927736451	[465,407]	[493,578]	7	hSpCas9	negative selection	4521251
9154483	9154505	5	-	27760321	MV411	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.27124394245500033	[652,581]	[576,361]	2	hSpCas9	negative selection	4604710
9190310	9190332	5	+	27760321	MV411	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.6910086276680864	[933,663]	[1089,469]	5	hSpCas9	negative selection	4604711
9190338	9190360	5	+	27760321	MV411	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	-0.2343456190994603	[643,534]	[164,575]	-2	hSpCas9	negative selection	4604712
9197206	9197228	5	-	27760321	MV411	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.573590449461454	[452,387]	[47,392]	-4	hSpCas9	negative selection	4604713
9226858	9226880	5	+	27760321	MV411	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.7802522185430815	[465,407]	[488,501]	6	hSpCas9	negative selection	4604714
9154483	9154505	5	-	27760321	HL60	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.946458650078041	[652,581]	[1291,629]	8	hSpCas9	negative selection	4688173
9190310	9190332	5	+	27760321	HL60	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.41982955704563374	[933,663]	[843,884]	5	hSpCas9	negative selection	4688174
9190338	9190360	5	+	27760321	HL60	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.30137208080776906	[643,534]	[602,577]	4	hSpCas9	negative selection	4688175
9197206	9197228	5	-	27760321	HL60	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.3890298367820768	[452,387]	[211,313]	-4	hSpCas9	negative selection	4688176
9226858	9226880	5	+	27760321	HL60	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.9502520718859652	[465,407]	[729,643]	8	hSpCas9	negative selection	4688177
9154483	9154505	5	-	27760321	HT1080	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	1.5115894845755538	[581,453]	[247,960]	8	hSpCas9	negative selection	4771636
9190310	9190332	5	+	27760321	HT1080	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.680475564309402	[663,517]	[246,519]	3	hSpCas9	negative selection	4771637
9190338	9190360	5	+	27760321	HT1080	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	-0.7191932422100115	[534,417]	[142,84]	-3	hSpCas9	negative selection	4771638
9197206	9197228	5	-	27760321	HT1080	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	1.1685871760626085	[387,302]	[317,299]	6	hSpCas9	negative selection	4771639
9226858	9226880	5	+	27760321	HT1080	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	1.0647872488752113	[407,318]	[185,430]	6	hSpCas9	negative selection	4771640
9154483	9154505	5	-	27760321	HT29	viability after 7 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.09931822609130081	[581,453]	[1069,656,470]	1	hSpCas9	negative selection	4855099
9190310	9190332	5	+	27760321	HT29	viability after 7 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.140020212063023	[663,517]	[2244,1577,1291]	9	hSpCas9	negative selection	4855100
9190338	9190360	5	+	27760321	HT29	viability after 7 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	-0.006875187509765901	[534,417]	[573,660,588]	0	hSpCas9	negative selection	4855101
9197206	9197228	5	-	27760321	HT29	viability after 7 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.07704839437593718	[387,302]	[586,391,310]	-1	hSpCas9	negative selection	4855102
9226858	9226880	5	+	27760321	HT29	viability after 7 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.4227586281397612	[407,318]	[577,608,682]	6	hSpCas9	negative selection	4855103
9154483	9154505	5	-	27760321	HT29	viability after 25 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.2962711209422475	[581,453]	[619,609,982]	3	hSpCas9	negative selection	4938562
9190310	9190332	5	+	27760321	HT29	viability after 25 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.3000912682468806	[663,517]	[1567,1828,1587]	9	hSpCas9	negative selection	4938563
9190338	9190360	5	+	27760321	HT29	viability after 25 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.6235267467818404	[534,417]	[766,873,883]	7	hSpCas9	negative selection	4938564
9197206	9197228	5	-	27760321	HT29	viability after 25 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	0.31083795346448717	[387,302]	[537,511,406]	3	hSpCas9	negative selection	4938565
9226858	9226880	5	+	27760321	HT29	viability after 25 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.4909659620383379	[407,318]	[585,456,721]	5	hSpCas9	negative selection	4938566
9154483	9154505	5	-	27760321	HT29	viability after 22 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.43421357971510377	[581,453]	[691,870,867]	5	hSpCas9	negative selection	5022025
9190310	9190332	5	+	27760321	HT29	viability after 22 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.1545012638096424	[663,517]	[1506,1421,1634]	9	hSpCas9	negative selection	5022026
9190338	9190360	5	+	27760321	HT29	viability after 22 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.37529424284749324	[534,417]	[682,801,658]	4	hSpCas9	negative selection	5022027
9197206	9197228	5	-	27760321	HT29	viability after 22 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.07615355655009008	[387,302]	[340,384,411]	-1	hSpCas9	negative selection	5022028
9226858	9226880	5	+	27760321	HT29	viability after 22 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.4268770242595459	[407,318]	[630,614,445]	4	hSpCas9	negative selection	5022029
9154483	9154505	5	-	27760321	HT29	viability after 19 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.6755056785679752	[581,453]	[1034,995,820]	7	hSpCas9	negative selection	5105488
9190310	9190332	5	+	27760321	HT29	viability after 19 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.059176048595132	[663,517]	[1444,1855,944]	9	hSpCas9	negative selection	5105489
9190338	9190360	5	+	27760321	HT29	viability after 19 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.6815401336406772	[534,417]	[1015,972,655]	7	hSpCas9	negative selection	5105490
9197206	9197228	5	-	27760321	HT29	viability after 19 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	0.34441931742171955	[387,302]	[519,278,699]	3	hSpCas9	negative selection	5105491
9226858	9226880	5	+	27760321	HT29	viability after 19 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.13783277220994192	[407,318]	[707,278,412]	1	hSpCas9	negative selection	5105492
9154483	9154505	5	-	27760321	HT29	viability after 16 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.2428875563669558	[581,453]	[704,572,783]	2	hSpCas9	negative selection	5188951
9190310	9190332	5	+	27760321	HT29	viability after 16 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.255637478957252	[663,517]	[1710,1597,1438]	9	hSpCas9	negative selection	5188952
9190338	9190360	5	+	27760321	HT29	viability after 16 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.3761948188529378	[534,417]	[605,488,977]	4	hSpCas9	negative selection	5188953
9197206	9197228	5	-	27760321	HT29	viability after 16 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.33568749867598413	[387,302]	[424,271,233]	-4	hSpCas9	negative selection	5188954
9226858	9226880	5	+	27760321	HT29	viability after 16 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.3372390600104142	[407,318]	[600,500,447]	4	hSpCas9	negative selection	5188955
9154483	9154505	5	-	27760321	HT29	viability after 13 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.037977805816134524	[581,453]	[494,738,616]	0	hSpCas9	negative selection	5272414
9190310	9190332	5	+	27760321	HT29	viability after 13 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	1.3353385839155123	[663,517]	[1993,1718,1490]	9	hSpCas9	negative selection	5272415
9190338	9190360	5	+	27760321	HT29	viability after 13 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.0675467545332516	[534,417]	[513,507,707]	0	hSpCas9	negative selection	5272416
9197206	9197228	5	-	27760321	HT29	viability after 13 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	-0.23164229105724188	[387,302]	[243,416,361]	-3	hSpCas9	negative selection	5272417
9226858	9226880	5	+	27760321	HT29	viability after 13 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.21702123074311647	[407,318]	[299,612,553]	2	hSpCas9	negative selection	5272418
9154483	9154505	5	-	27760321	HT29	viability after 10 days	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.15679249684857466	[581,453]	[755,748,659]	2	hSpCas9	negative selection	5355877
9190310	9190332	5	+	27760321	HT29	viability after 10 days	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.9525642707145197	[663,517]	[1334,1352,1589]	9	hSpCas9	negative selection	5355878
9190338	9190360	5	+	27760321	HT29	viability after 10 days	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.4404857554537571	[534,417]	[1001,777,644]	6	hSpCas9	negative selection	5355879
9197206	9197228	5	-	27760321	HT29	viability after 10 days	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	0.18669810679365978	[387,302]	[568,337,559]	2	hSpCas9	negative selection	5355880
9226858	9226880	5	+	27760321	HT29	viability after 10 days	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.32337196226020826	[407,318]	[539,705,463]	4	hSpCas9	negative selection	5355881
9154483	9154505	5	-	27760321	MOLM13	viability	TTCTACCGCACACGCAGGTAGG	SEMA5A	ENSG00000112902	0.5513711889919808	[652,581]	[491,524]	4	hSpCas9	negative selection	5439340
9190310	9190332	5	+	27760321	MOLM13	viability	CACCACGTCGACTGCCACGTGG	SEMA5A	ENSG00000112902	0.7915237739497797	[933,663]	[717,817]	5	hSpCas9	negative selection	5439341
9190338	9190360	5	+	27760321	MOLM13	viability	CGGCTATTGTCCTCCATGAAGG	SEMA5A	ENSG00000112902	0.532021642114664	[643,534]	[332,602]	3	hSpCas9	negative selection	5439342
9197206	9197228	5	-	27760321	MOLM13	viability	TACCAAGAAAACTCGCGCTCGG	SEMA5A	ENSG00000112902	0.8372497296462575	[452,387]	[582,285]	6	hSpCas9	negative selection	5439343
9226858	9226880	5	+	27760321	MOLM13	viability	AGAAGCCATACCGAGCGGTTGG	SEMA5A	ENSG00000112902	0.7996494128095919	[465,407]	[424,430]	5	hSpCas9	negative selection	5439344
9545985	9546008	5	+	27661255	K562	viability	GAGGCTGCGAAGCCCACCCGCGG	SEMA5A	ENSG00000112902	0.043218114320000445	[1482,1410]	[1224,1163]	1	dCas9-KRAB	negative selection	5510060
9546004	9546027	5	+	27661255	K562	viability	GCGGCGGGAAAGCAGCGCTCGGG	SEMA5A	ENSG00000112902	0.9326294181151855	[36,97]	[124,80]	9	dCas9-KRAB	negative selection	5510061
9546009	9546032	5	+	27661255	K562	viability	GGGAAAGCAGCGCTCGGGAGCGG	SEMA5A	ENSG00000112902	-0.08215265872456307	[612,555]	[477,410]	-3	dCas9-KRAB	negative selection	5510062
9545605	9545628	5	-	27661255	K562	viability	GCCCCCAGGCGCTGGATGACTGG	SEMA5A	ENSG00000112902	-0.04112398872054057	[359,397]	[317,272]	-2	dCas9-KRAB	negative selection	5510063
9337781	9337804	5	+	27260156	BXPC3	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.3575567499335836	[428]	[446,550,650,674]	7	hSpCas9	negative selection	5569448
9337768	9337791	5	-	27260156	BXPC3	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.2674239664104028	[47]	[55,28,100,57]	6	hSpCas9	negative selection	5633702
9318389	9318412	5	+	27260156	BXPC3	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.3420180859265486	[226]	[272,353,194,387]	7	hSpCas9	negative selection	5633703
9318402	9318425	5	-	27260156	BXPC3	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.0398874778867202	[1265]	[1111,1289,1273,1847]	0	hSpCas9	negative selection	5633704
9337781	9337804	5	+	27260156	A673	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.12326706825924277	[428]	[591,679,439,530]	3	hSpCas9	negative selection	5690699
9337768	9337791	5	-	27260156	A673	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.1584838471036898	[47]	[73,26,49,110]	4	hSpCas9	negative selection	5754953
9318389	9318412	5	+	27260156	A673	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.04599144102141328	[226]	[322,266,175,298]	-1	hSpCas9	negative selection	5754954
9318402	9318425	5	-	27260156	A673	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.06349703557913355	[1265]	[1661,1500,1616,1580]	1	hSpCas9	negative selection	5754955
9337781	9337804	5	+	27260156	A375	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.6108451882758541	[428]	[682,796,911,679]	8	hSpCas9	negative selection	5811950
9337768	9337791	5	-	27260156	A375	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.1714137635285442	[47]	[90,110,47,14]	2	hSpCas9	negative selection	5876204
9318389	9318412	5	+	27260156	A375	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.5263145767292023	[226]	[155,195,153,225]	-6	hSpCas9	negative selection	5876205
9318402	9318425	5	-	27260156	A375	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.33466217598422965	[1265]	[2176,1721,1872,1683]	5	hSpCas9	negative selection	5876206
9337781	9337804	5	+	27260156	COLO741	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.4933906054916845	[428]	[720,704,874]	8	hSpCas9	negative selection	5933201
9337768	9337791	5	-	27260156	COLO741	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.528957830792729	[47]	[79,101,82]	9	hSpCas9	negative selection	5997455
9318389	9318412	5	+	27260156	COLO741	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.07258289985424338	[226]	[347,363,212]	1	hSpCas9	negative selection	5997456
9318402	9318425	5	-	27260156	COLO741	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.47713144372042815	[1265]	[2994,2298,1569]	8	hSpCas9	negative selection	5997457
9337781	9337804	5	+	27260156	CAL120	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.04815555055185804	[428]	[616,359,685]	0	hSpCas9	negative selection	6054452
9337768	9337791	5	-	27260156	CAL120	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.20631241335890937	[47]	[77,71,9]	-3	hSpCas9	negative selection	6118706
9318389	9318412	5	+	27260156	CAL120	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.14003975130879542	[226]	[285,320,167]	-2	hSpCas9	negative selection	6118707
9318402	9318425	5	-	27260156	CAL120	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.19935825690719744	[1265]	[1822,2079,1529]	3	hSpCas9	negative selection	6118708
9337781	9337804	5	+	27260156	CADOES1	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.2773414875419147	[428]	[437,471,523,500]	5	hSpCas9	negative selection	6175703
9337768	9337791	5	-	27260156	CADOES1	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.08339771369090876	[47]	[29,54,37,66]	1	hSpCas9	negative selection	6239957
9318389	9318412	5	+	27260156	CADOES1	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.36781023562483917	[226]	[250,277,282,277]	6	hSpCas9	negative selection	6239958
9318402	9318425	5	-	27260156	CADOES1	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.36181597496615714	[1265]	[1544,1756,1308,1438]	6	hSpCas9	negative selection	6239959
9337781	9337804	5	+	27260156	EWS502	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.11987780893788459	[428]	[488,680,497,282]	2	hSpCas9	negative selection	6296954
9337768	9337791	5	-	27260156	EWS502	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.4898414945968943	[47]	[55,74,39,118]	8	hSpCas9	negative selection	6361208
9318389	9318412	5	+	27260156	EWS502	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.44316736220323305	[226]	[286,295,442,284]	7	hSpCas9	negative selection	6361209
9318402	9318425	5	-	27260156	EWS502	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.21420243367703584	[1265]	[1245,1472,1668,1894]	4	hSpCas9	negative selection	6361210
9337781	9337804	5	+	27260156	EW8	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.3656807232057625	[428]	[410,549,406,425]	6	hSpCas9	negative selection	6418205
9337768	9337791	5	-	27260156	EW8	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.23306306934524956	[47]	[51,78,19,36]	4	hSpCas9	negative selection	6482459
9318389	9318412	5	+	27260156	EW8	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.026888697953711516	[226]	[157,207,110,253]	0	hSpCas9	negative selection	6482460
9318402	9318425	5	-	27260156	EW8	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.12340466456269739	[1265]	[1216,1161,1013,1102]	2	hSpCas9	negative selection	6482461
9337781	9337804	5	+	27260156	CORL105	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.6834739080355465	[428]	[761,709,1132,672]	9	hSpCas9	negative selection	6539456
9337768	9337791	5	-	27260156	CORL105	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.39985370257107977	[47]	[68,57,107,66]	7	hSpCas9	negative selection	6603710
9318389	9318412	5	+	27260156	CORL105	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.027220347251753374	[226]	[297,221,214,340]	0	hSpCas9	negative selection	6603711
9318402	9318425	5	-	27260156	CORL105	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.17719487028989134	[1265]	[1890,1358,1952,1556]	3	hSpCas9	negative selection	6603712
9337781	9337804	5	+	27260156	HS294T	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.777508823675835	[428]	[310,314,265,231]	8	hSpCas9	negative selection	6660707
9337768	9337791	5	-	27260156	HS294T	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.34123709938621793	[47]	[12,15,43,21]	4	hSpCas9	negative selection	6724961
9318389	9318412	5	+	27260156	HS294T	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.08235088894430354	[226]	[44,157,86,81]	1	hSpCas9	negative selection	6724962
9318402	9318425	5	-	27260156	HS294T	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.23256533691579717	[1265]	[656,566,365,725]	3	hSpCas9	negative selection	6724963
9337781	9337804	5	+	27260156	HCC44	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.3685882022173483	[428]	[510,375,312,303]	-5	hSpCas9	negative selection	6781958
9337768	9337791	5	-	27260156	HCC44	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.6854050284270938	[47]	[0,37,36,54]	-7	hSpCas9	negative selection	6846212
9318389	9318412	5	+	27260156	HCC44	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.9628051270791969	[226]	[140,125,143,118]	-8	hSpCas9	negative selection	6846213
9318402	9318425	5	-	27260156	HCC44	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.3476821172086382	[1265]	[1902,1853,2032,1531]	5	hSpCas9	negative selection	6846214
9337781	9337804	5	+	27260156	G402	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.18836556713844907	[428]	[945,528,376,578]	2	hSpCas9	negative selection	6903209
9337768	9337791	5	-	27260156	G402	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-1.1548742731628256	[47]	[61,24,13,9]	-8	hSpCas9	negative selection	6967463
9318389	9318412	5	+	27260156	G402	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.1578659123402144	[226]	[476,385,184,219]	2	hSpCas9	negative selection	6967464
9318402	9318425	5	-	27260156	G402	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.622529843830826	[1265]	[2234,3434,1736,2148]	7	hSpCas9	negative selection	6967465
9337781	9337804	5	+	27260156	L33	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.5500395137527231	[428]	[535,281,367,703]	9	hSpCas9	negative selection	7024460
9337768	9337791	5	-	27260156	L33	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.32205960599426176	[47]	[112,12,19,61]	6	hSpCas9	negative selection	7088714
9318389	9318412	5	+	27260156	L33	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.0019671796944542574	[226]	[215,95,181,167]	0	hSpCas9	negative selection	7088715
9318402	9318425	5	-	27260156	L33	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	-0.19658739358733546	[1265]	[1009,457,655,1247]	-4	hSpCas9	negative selection	7088716
9337781	9337804	5	+	27260156	K562	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.0858211979410375	[428]	[490,421,479,247]	1	hSpCas9	negative selection	7145711
9337768	9337791	5	-	27260156	K562	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.12367652303245702	[47]	[36,66,65,18]	1	hSpCas9	negative selection	7209965
9318389	9318412	5	+	27260156	K562	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	1.8854816993693477	[226]	[630,258,1745,349]	9	hSpCas9	negative selection	7209966
9318402	9318425	5	-	27260156	K562	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.7380564951088744	[1265]	[1854,2002,1913,1736]	8	hSpCas9	negative selection	7209967
9337781	9337804	5	+	27260156	HT29	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.20637373781552887	[428]	[660,598,726,439]	4	hSpCas9	negative selection	7266962
9337768	9337791	5	-	27260156	HT29	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.2038501284022991	[47]	[89,38,21,52]	-4	hSpCas9	negative selection	7331216
9318389	9318412	5	+	27260156	HT29	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.10728682006402523	[226]	[254,196,416,304]	2	hSpCas9	negative selection	7331217
9318402	9318425	5	-	27260156	HT29	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.13385371048472283	[1265]	[1722,1434,1966,1607]	2	hSpCas9	negative selection	7331218
9337781	9337804	5	+	27260156	MHHES1	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.5464096977326176	[428]	[793,781,731,552]	8	hSpCas9	negative selection	7388213
9337768	9337791	5	-	27260156	MHHES1	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.03792748249227618	[47]	[27,73,55,50]	0	hSpCas9	negative selection	7452467
9318389	9318412	5	+	27260156	MHHES1	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.5085696876239244	[226]	[382,435,300,340]	8	hSpCas9	negative selection	7452468
9318402	9318425	5	-	27260156	MHHES1	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.31414129519128475	[1265]	[1947,1963,2264,1080]	6	hSpCas9	negative selection	7452469
9337781	9337804	5	+	27260156	MEWO	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.14308378576680728	[428]	[539,496,310]	3	hSpCas9	negative selection	7509464
9337768	9337791	5	-	27260156	MEWO	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.7397917027611531	[47]	[105,67,56]	9	hSpCas9	negative selection	7573718
9318389	9318412	5	+	27260156	MEWO	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.2127277544260775	[226]	[199,177,174]	-5	hSpCas9	negative selection	7573719
9318402	9318425	5	-	27260156	MEWO	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	-0.04225454417014096	[1265]	[1320,1053,1108]	-1	hSpCas9	negative selection	7573720
9337781	9337804	5	+	27260156	LNCAPCLONEFGC	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.2821522784906954	[428]	[480,429,542,448]	4	hSpCas9	negative selection	7630715
9337768	9337791	5	-	27260156	LNCAPCLONEFGC	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.20080997032331327	[47]	[42,5,55,50]	-3	hSpCas9	negative selection	7694969
9318389	9318412	5	+	27260156	LNCAPCLONEFGC	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.4192586617272067	[226]	[124,151,209,136]	-6	hSpCas9	negative selection	7694970
9318402	9318425	5	-	27260156	LNCAPCLONEFGC	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.37381883748082223	[1265]	[1578,1449,1555,1368]	6	hSpCas9	negative selection	7694971
9337781	9337804	5	+	27260156	PANC0327	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.2649522824336781	[428]	[895,196,383,423]	4	hSpCas9	negative selection	7751966
9337768	9337791	5	-	27260156	PANC0327	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.32898711788177426	[47]	[37,75,28,66]	5	hSpCas9	negative selection	7816220
9318389	9318412	5	+	27260156	PANC0327	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.20830700589976747	[226]	[197,202,334,196]	3	hSpCas9	negative selection	7816221
9318402	9318425	5	-	27260156	PANC0327	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.35904676880135833	[1265]	[1231,1378,1501,1646]	5	hSpCas9	negative selection	7816222
9337781	9337804	5	+	27260156	NCIH2009	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.4769855709731001	[428]	[1142,796,622]	6	hSpCas9	negative selection	7873217
9337768	9337791	5	-	27260156	NCIH2009	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.2229675505261146	[47]	[138,60,46]	3	hSpCas9	negative selection	7937471
9318389	9318412	5	+	27260156	NCIH2009	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.3922035873801386	[226]	[302,279,152]	-5	hSpCas9	negative selection	7937472
9318402	9318425	5	-	27260156	NCIH2009	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.030321078689910852	[1265]	[1766,1489,2156]	0	hSpCas9	negative selection	7937473
9337781	9337804	5	+	27260156	NCIH1373	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	1.0650299228363747	[428]	[1009,654,1743,159]	9	hSpCas9	negative selection	7994468
9337768	9337791	5	-	27260156	NCIH1373	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	1.1957731119060142	[47]	[8,326,10,27]	9	hSpCas9	negative selection	8058722
9318389	9318412	5	+	27260156	NCIH1373	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.23918462859875178	[226]	[445,149,135,44]	-2	hSpCas9	negative selection	8058723
9318402	9318425	5	-	27260156	NCIH1373	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.5541590525491524	[1265]	[2139,2276,1869,531]	5	hSpCas9	negative selection	8058724
9337781	9337804	5	+	27260156	PATU8902	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.7042394432231431	[428]	[1222,1049,1012,533]	7	hSpCas9	negative selection	8115719
9337768	9337791	5	-	27260156	PATU8902	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.3326478834100799	[47]	[186,4,32,127]	3	hSpCas9	negative selection	8179973
9318389	9318412	5	+	27260156	PATU8902	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.349194920394326	[226]	[172,317,439,705]	3	hSpCas9	negative selection	8179974
9318402	9318425	5	-	27260156	PATU8902	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.7201386801893099	[1265]	[3023,2320,2752,3617]	7	hSpCas9	negative selection	8179975
9337781	9337804	5	+	27260156	PANC1	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.02527614107208523	[428]	[450,279,388,311]	0	hSpCas9	negative selection	8236970
9337768	9337791	5	-	27260156	PANC1	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.4957675078207493	[47]	[46,54,75,53]	7	hSpCas9	negative selection	8301224
9318389	9318412	5	+	27260156	PANC1	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.15167752441506188	[226]	[109,351,123,153]	-2	hSpCas9	negative selection	8301225
9318402	9318425	5	-	27260156	PANC1	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.3415633551440749	[1265]	[1298,1673,1533,1092]	5	hSpCas9	negative selection	8301226
9337781	9337804	5	+	27260156	PANC0813	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.4793905202423007	[428]	[788,712,653,662]	7	hSpCas9	negative selection	8358221
9337768	9337791	5	-	27260156	PANC0813	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.05394696712596536	[47]	[77,50,51,54]	0	hSpCas9	negative selection	8422475
9318389	9318412	5	+	27260156	PANC0813	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.1459343027148664	[226]	[203,251,354,356]	2	hSpCas9	negative selection	8422476
9318402	9318425	5	-	27260156	PANC0813	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	-0.020011297490889257	[1265]	[1721,1542,1530,1111]	0	hSpCas9	negative selection	8422477
9337781	9337804	5	+	27260156	RDES	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.22007299785670092	[428]	[574,497,566,658]	3	hSpCas9	negative selection	8479472
9337768	9337791	5	-	27260156	RDES	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.05786184050876564	[47]	[64,23,72,69]	0	hSpCas9	negative selection	8543726
9318389	9318412	5	+	27260156	RDES	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.13106766048733143	[226]	[236,233,344,345]	2	hSpCas9	negative selection	8543727
9318402	9318425	5	-	27260156	RDES	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.31742244044062096	[1265]	[1604,1607,1896,2219]	5	hSpCas9	negative selection	8543728
9337781	9337804	5	+	27260156	PC3	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.1843750395083846	[428]	[401,383,826,443]	4	hSpCas9	negative selection	8600723
9337768	9337791	5	-	27260156	PC3	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.2447578884897319	[47]	[26,45,140,54]	5	hSpCas9	negative selection	8664977
9318389	9318412	5	+	27260156	PC3	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.09795478327623997	[226]	[111,252,342,211]	-2	hSpCas9	negative selection	8664978
9318402	9318425	5	-	27260156	PC3	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.021550448293654823	[1265]	[994,1118,2002,1270]	0	hSpCas9	negative selection	8664979
9337781	9337804	5	+	27260156	PATU8988T	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	-0.02263328473886944	[428]	[447,361,467,343]	0	hSpCas9	negative selection	8721974
9337768	9337791	5	-	27260156	PATU8988T	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.2688747871486341	[47]	[12,30,97,7]	-4	hSpCas9	negative selection	8786228
9318389	9318412	5	+	27260156	PATU8988T	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.3956063671359986	[226]	[87,136,228,212]	-6	hSpCas9	negative selection	8786229
9318402	9318425	5	-	27260156	PATU8988T	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.21414285087427487	[1265]	[1294,1444,1577,1352]	3	hSpCas9	negative selection	8786230
9337781	9337804	5	+	27260156	T47D	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.039352923283446806	[428]	[551,416,472,624]	0	hSpCas9	negative selection	8843225
9337768	9337791	5	-	27260156	T47D	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.09896830281264402	[47]	[37,42,79,47]	-2	hSpCas9	negative selection	8907479
9318389	9318412	5	+	27260156	T47D	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.1840723424856402	[226]	[268,325,328,273]	4	hSpCas9	negative selection	8907480
9318402	9318425	5	-	27260156	T47D	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.16214006633797684	[1265]	[1795,1645,1527,1632]	3	hSpCas9	negative selection	8907481
9337781	9337804	5	+	27260156	SU8686	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.820688485695348	[428]	[567,579,1103,809]	9	hSpCas9	negative selection	8964476
9337768	9337791	5	-	27260156	SU8686	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.4417157466666781	[47]	[41,42,20,30]	-6	hSpCas9	negative selection	9028730
9318389	9318412	5	+	27260156	SU8686	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	-0.09741524364389775	[226]	[173,240,196,230]	-2	hSpCas9	negative selection	9028731
9318402	9318425	5	-	27260156	SU8686	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.1057978534093329	[1265]	[1211,1122,1437,1728]	1	hSpCas9	negative selection	9028732
9337781	9337804	5	+	27260156	SKES1	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.4041582481836553	[428]	[657,675,485,476]	8	hSpCas9	negative selection	9085727
9337768	9337791	5	-	27260156	SKES1	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.3690438874748372	[47]	[43,74,56,68]	7	hSpCas9	negative selection	9149981
9318389	9318412	5	+	27260156	SKES1	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.007859080975879174	[226]	[209,338,191,183]	0	hSpCas9	negative selection	9149982
9318402	9318425	5	-	27260156	SKES1	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.29278298659338686	[1265]	[1653,1749,1682,1167]	6	hSpCas9	negative selection	9149983
9337781	9337804	5	+	27260156	TOV112D	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.45980650443032234	[428]	[572,614,580,597]	8	hSpCas9	negative selection	9206978
9337768	9337791	5	-	27260156	TOV112D	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.1615771935751944	[47]	[47,103,17,17]	-3	hSpCas9	negative selection	9271232
9318389	9318412	5	+	27260156	TOV112D	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.7749863332949611	[226]	[390,324,292,526]	9	hSpCas9	negative selection	9271233
9318402	9318425	5	-	27260156	TOV112D	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.1833044170659779	[1265]	[1364,1663,1354,1421]	3	hSpCas9	negative selection	9271234
9337781	9337804	5	+	27260156	TC71	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.0494950016011515	[428]	[457,421,532,489]	1	hSpCas9	negative selection	9328229
9337768	9337791	5	-	27260156	TC71	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	0.11587571103427141	[47]	[87,23,47,62]	3	hSpCas9	negative selection	9392483
9318389	9318412	5	+	27260156	TC71	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.3619346823384608	[226]	[325,337,242,347]	8	hSpCas9	negative selection	9392484
9318402	9318425	5	-	27260156	TC71	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.08720334997488721	[1265]	[1297,1448,1478,1548]	2	hSpCas9	negative selection	9392485
9337781	9337804	5	+	27260156	TC32	viability	CGCTCTGAACTCCCGTAACCAGG	SEMA5A	ENSG00000112902	0.2962391522062285	[428]	[506,366,695,459]	5	hSpCas9	negative selection	9449480
9337768	9337791	5	-	27260156	TC32	viability	GTTCAGAGCGAAGAATGCTGTGG	SEMA5A	ENSG00000112902	-0.7083422804001214	[47]	[35,1,41,40]	-8	hSpCas9	negative selection	9513734
9318389	9318412	5	+	27260156	TC32	viability	CCTCAAGCTGTAACCTGAAGAGG	SEMA5A	ENSG00000112902	0.09149866181268429	[226]	[221,170,270,262]	1	hSpCas9	negative selection	9513735
9318402	9318425	5	-	27260156	TC32	viability	TTTGCAGAAACTACCTCTTCAGG	SEMA5A	ENSG00000112902	0.38711167911854405	[1265]	[1482,1037,1966,1943]	6	hSpCas9	negative selection	9513736
9197248	9197271	5	-	27869803	HPAFII	viability after 27 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.1286357000572518	[137]	[252,79,NaN]	8	hSpCas9	negative selection	9592840
9202112	9202135	5	+	27869803	HPAFII	viability after 27 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.17645638854520154	[210]	[180,78,NaN]	1	hSpCas9	negative selection	9592841
9224681	9224704	5	-	27869803	HPAFII	viability after 27 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.9725018205070575	[617]	[558,702,NaN]	7	hSpCas9	negative selection	9592842
9226920	9226943	5	-	27869803	HPAFII	viability after 27 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.22593290141216674	[257]	[138,174,NaN]	1	hSpCas9	negative selection	9592843
9337782	9337805	5	+	27869803	HPAFII	viability after 27 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.16328234425544244	[279]	[179,90,NaN]	-1	hSpCas9	negative selection	9592857
9197248	9197271	5	-	27869803	HPAFII	viability after 15 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.1517210052678184	[137]	[532,202,NaN]	9	hSpCas9	negative selection	9675289
9202112	9202135	5	+	27869803	HPAFII	viability after 15 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.2645618206461669	[210]	[375,207,NaN]	3	hSpCas9	negative selection	9675290
9224681	9224704	5	-	27869803	HPAFII	viability after 15 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.20196148938867142	[617]	[771,758,NaN]	2	hSpCas9	negative selection	9675291
9226920	9226943	5	-	27869803	HPAFII	viability after 15 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.762914382020575	[257]	[517,440,NaN]	8	hSpCas9	negative selection	9675292
9337782	9337805	5	+	27869803	HPAFII	viability after 15 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.3248275471708473	[279]	[304,199,NaN]	-4	hSpCas9	negative selection	9675306
9197248	9197271	5	-	27869803	ASPC1	viability after 36 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.388798106840695	[499]	[243,923,230]	9	hSpCas9	negative selection	9757738
9202112	9202135	5	+	27869803	ASPC1	viability after 36 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.7180588006132381	[440]	[120,201,383]	7	hSpCas9	negative selection	9757739
9224681	9224704	5	-	27869803	ASPC1	viability after 36 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.4596992294347836	[1060]	[984,316,322]	4	hSpCas9	negative selection	9757740
9226920	9226943	5	-	27869803	ASPC1	viability after 36 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.6023085540198405	[928]	[472,467,519]	6	hSpCas9	negative selection	9757741
9337782	9337805	5	+	27869803	ASPC1	viability after 36 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.5145103497700657	[620]	[142,136,167]	-5	hSpCas9	negative selection	9757755
9197248	9197271	5	-	27869803	PATU8988S	viability after 35 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.9379643276242668	[379]	[383,201,NaN]	8	hSpCas9	negative selection	9840187
9202112	9202135	5	+	27869803	PATU8988S	viability after 35 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.14106558765808064	[545]	[237,246,NaN]	1	hSpCas9	negative selection	9840188
9224681	9224704	5	-	27869803	PATU8988S	viability after 35 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.31622262133819545	[810]	[483,328,NaN]	3	hSpCas9	negative selection	9840189
9226920	9226943	5	-	27869803	PATU8988S	viability after 35 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.6183700848871876	[737]	[244,141,NaN]	-6	hSpCas9	negative selection	9840190
9337782	9337805	5	+	27869803	PATU8988S	viability after 35 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.13438543867196118	[527]	[194,271,NaN]	1	hSpCas9	negative selection	9840204
9197248	9197271	5	-	27869803	PATU8988S	viability after 31 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	0.4970080826855357	[379]	[219,205,NaN]	6	hSpCas9	negative selection	9922636
9202112	9202135	5	+	27869803	PATU8988S	viability after 31 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.16036575550387758	[545]	[197,300,NaN]	2	hSpCas9	negative selection	9922637
9224681	9224704	5	-	27869803	PATU8988S	viability after 31 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.2835071337765468	[810]	[408,373,NaN]	3	hSpCas9	negative selection	9922638
9226920	9226943	5	-	27869803	PATU8988S	viability after 31 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	-0.3993765994493834	[737]	[190,263,NaN]	-4	hSpCas9	negative selection	9922639
9337782	9337805	5	+	27869803	PATU8988S	viability after 31 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.07438155625906623	[527]	[184,218,NaN]	-1	hSpCas9	negative selection	9922653
9197248	9197271	5	-	27869803	HPAFII	viability after 35 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.3831748259108791	[137]	[288,61,NaN]	8	hSpCas9	negative selection	10005085
9202112	9202135	5	+	27869803	HPAFII	viability after 35 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	-1.1325871346362106	[210]	[75,17,NaN]	-6	hSpCas9	negative selection	10005086
9224681	9224704	5	-	27869803	HPAFII	viability after 35 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	1.0172186154787906	[617]	[390,905,NaN]	7	hSpCas9	negative selection	10005087
9226920	9226943	5	-	27869803	HPAFII	viability after 35 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.39324190289412003	[257]	[68,286,NaN]	2	hSpCas9	negative selection	10005088
9337782	9337805	5	+	27869803	HPAFII	viability after 35 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	0.219887863613785	[279]	[221,100,NaN]	1	hSpCas9	negative selection	10005102
9197248	9197271	5	-	27869803	HPAFII	viability after 31 days	CAACCTGAGCGCCATCGCGCAGG	SEMA5A	ENSG00000112902	1.4213175588352351	[137]	[283,123,NaN]	9	hSpCas9	negative selection	10087534
9202112	9202135	5	+	27869803	HPAFII	viability after 31 days	CAATATCGTTCTTGCACACCCGG	SEMA5A	ENSG00000112902	0.15650998605007205	[210]	[220,34,NaN]	1	hSpCas9	negative selection	10087535
9224681	9224704	5	-	27869803	HPAFII	viability after 31 days	CGGCGCAGTACAACTCCAAATGG	SEMA5A	ENSG00000112902	0.8144046604840816	[617]	[451,781,NaN]	6	hSpCas9	negative selection	10087536
9226920	9226943	5	-	27869803	HPAFII	viability after 31 days	TGCTTCTGGTGGGTGGCGACCGG	SEMA5A	ENSG00000112902	0.16364688307133857	[257]	[76,254,NaN]	1	hSpCas9	negative selection	10087537
9337782	9337805	5	+	27869803	HPAFII	viability after 31 days	GCTCTGAACTCCCGTAACCAGGG	SEMA5A	ENSG00000112902	-0.23182348439317818	[279]	[151,113,NaN]	-1	hSpCas9	negative selection	10087551
9044516	9044539	5	+	28162770	P31FUJ	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-1.1242777476362096	[127]	[68]	-7	hSpCas9	negative selection	10231777
9226943	9226966	5	-	28162770	P31FUJ	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.2550378294086728	[255]	[358]	2	hSpCas9	negative selection	10231778
9197267	9197290	5	+	28162770	P31FUJ	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.10016443583625953	[269]	[295]	-1	hSpCas9	negative selection	10231779
9136551	9136574	5	-	28162770	P31FUJ	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.1334397910296211	[217]	[280]	1	hSpCas9	negative selection	10231780
9136528	9136551	5	-	28162770	P31FUJ	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.5041633897518274	[194]	[324]	6	hSpCas9	negative selection	10231781
9108202	9108225	5	-	28162770	P31FUJ	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.18145609147480246	[462]	[616]	2	hSpCas9	negative selection	10231782
9066552	9066575	5	-	28162770	P31FUJ	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.4438490259986316	[126]	[202]	5	hSpCas9	negative selection	10231783
9054200	9054223	5	-	28162770	P31FUJ	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.0805298667845986	[201]	[250]	0	hSpCas9	negative selection	10231784
9054139	9054162	5	+	28162770	P31FUJ	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.5983208835761752	[85]	[152]	6	hSpCas9	negative selection	10231785
9044516	9044539	5	+	28162770	TF1	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[85]	1	hSpCas9	negative selection	10401143
9226943	9226966	5	-	28162770	TF1	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[260]	1	hSpCas9	negative selection	10401144
9197267	9197290	5	+	28162770	TF1	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[191]	1	hSpCas9	negative selection	10401145
9136551	9136574	5	-	28162770	TF1	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[168]	1	hSpCas9	negative selection	10401146
9136528	9136551	5	-	28162770	TF1	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[292]	1	hSpCas9	negative selection	10401147
9108202	9108225	5	-	28162770	TF1	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[296]	1	hSpCas9	negative selection	10401148
9066552	9066575	5	-	28162770	TF1	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[24]	1	hSpCas9	negative selection	10401149
9054200	9054223	5	-	28162770	TF1	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[159]	1	hSpCas9	negative selection	10401150
9054139	9054162	5	+	28162770	TF1	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	NaN	[NaN]	[83]	1	hSpCas9	negative selection	10401151
9044516	9044539	5	+	28162770	SKM1	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.5925198518992383	[28]	[33]	-5	hSpCas9	negative selection	10570509
9226943	9226966	5	-	28162770	SKM1	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.15630323087867548	[66]	[131]	1	hSpCas9	negative selection	10570510
9197267	9197290	5	+	28162770	SKM1	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.4210393839548845	[102]	[135]	-3	hSpCas9	negative selection	10570511
9136551	9136574	5	-	28162770	SKM1	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.21091292445072946	[64]	[132]	2	hSpCas9	negative selection	10570512
9136528	9136551	5	-	28162770	SKM1	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.2704445507425897	[67]	[144]	2	hSpCas9	negative selection	10570513
9108202	9108225	5	-	28162770	SKM1	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.152311658314345	[84]	[166]	1	hSpCas9	negative selection	10570514
9066552	9066575	5	-	28162770	SKM1	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.23046572187213	[26]	[55]	2	hSpCas9	negative selection	10570515
9054200	9054223	5	-	28162770	SKM1	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.14885195631847803	[24]	[48]	1	hSpCas9	negative selection	10570516
9054139	9054162	5	+	28162770	SKM1	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	-0.8220016980220053	[20]	[20]	-6	hSpCas9	negative selection	10570517
9044516	9044539	5	+	28162770	SKM1	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.5925198518992383	[28]	[33]	-5	hSpCas9	negative selection	10739875
9226943	9226966	5	-	28162770	SKM1	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.15630323087867548	[66]	[131]	1	hSpCas9	negative selection	10739876
9197267	9197290	5	+	28162770	SKM1	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.4210393839548845	[102]	[135]	-3	hSpCas9	negative selection	10739877
9136551	9136574	5	-	28162770	SKM1	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.21091292445072946	[64]	[132]	2	hSpCas9	negative selection	10739878
9136528	9136551	5	-	28162770	SKM1	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.2704445507425897	[67]	[144]	2	hSpCas9	negative selection	10739879
9108202	9108225	5	-	28162770	SKM1	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.152311658314345	[84]	[166]	1	hSpCas9	negative selection	10739880
9066552	9066575	5	-	28162770	SKM1	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.23046572187213	[26]	[55]	2	hSpCas9	negative selection	10739881
9054200	9054223	5	-	28162770	SKM1	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.14885195631847803	[24]	[48]	1	hSpCas9	negative selection	10739882
9054139	9054162	5	+	28162770	SKM1	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	-0.8220016980220053	[20]	[20]	-6	hSpCas9	negative selection	10739883
9044516	9044539	5	+	28162770	PL21	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-4.209251321648382	[84]	[1]	-9	hSpCas9	negative selection	10909241
9226943	9226966	5	-	28162770	PL21	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.4030530278855431	[244]	[140]	3	hSpCas9	negative selection	10909242
9197267	9197290	5	+	28162770	PL21	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.3887593007910064	[366]	[121]	-3	hSpCas9	negative selection	10909243
9136551	9136574	5	-	28162770	PL21	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.09446153659478307	[268]	[124]	0	hSpCas9	negative selection	10909244
9136528	9136551	5	-	28162770	PL21	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.6362387292959935	[203]	[137]	5	hSpCas9	negative selection	10909245
9108202	9108225	5	-	28162770	PL21	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.20013961448932016	[279]	[139]	1	hSpCas9	negative selection	10909246
9066552	9066575	5	-	28162770	PL21	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	-1.121788480398042	[94]	[18]	-6	hSpCas9	negative selection	10909247
9054200	9054223	5	-	28162770	PL21	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	-0.4736321531896921	[133]	[41]	-3	hSpCas9	negative selection	10909248
9054139	9054162	5	+	28162770	PL21	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	-1.1354634172951186	[105]	[20]	-6	hSpCas9	negative selection	10909249
9044516	9044539	5	+	28162770	P31FUJ	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-1.1242777476362096	[127]	[68]	-7	hSpCas9	negative selection	11078607
9226943	9226966	5	-	28162770	P31FUJ	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.2550378294086728	[255]	[358]	2	hSpCas9	negative selection	11078608
9197267	9197290	5	+	28162770	P31FUJ	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.10016443583625953	[269]	[295]	-1	hSpCas9	negative selection	11078609
9136551	9136574	5	-	28162770	P31FUJ	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.1334397910296211	[217]	[280]	1	hSpCas9	negative selection	11078610
9136528	9136551	5	-	28162770	P31FUJ	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.5041633897518274	[194]	[324]	6	hSpCas9	negative selection	11078611
9108202	9108225	5	-	28162770	P31FUJ	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.18145609147480246	[462]	[616]	2	hSpCas9	negative selection	11078612
9066552	9066575	5	-	28162770	P31FUJ	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.4438490259986316	[126]	[202]	5	hSpCas9	negative selection	11078613
9054200	9054223	5	-	28162770	P31FUJ	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.0805298667845986	[201]	[250]	0	hSpCas9	negative selection	11078614
9054139	9054162	5	+	28162770	P31FUJ	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.5983208835761752	[85]	[152]	6	hSpCas9	negative selection	11078615
9044516	9044539	5	+	28162770	OCIAML5	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	0.044428853382142974	[64]	[120]	0	hSpCas9	negative selection	11247973
9226943	9226966	5	-	28162770	OCIAML5	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.8542022260792927	[132]	[433]	7	hSpCas9	negative selection	11247974
9197267	9197290	5	+	28162770	OCIAML5	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.559459049646856	[168]	[206]	-4	hSpCas9	negative selection	11247975
9136551	9136574	5	-	28162770	OCIAML5	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.34269028355825104	[127]	[292]	3	hSpCas9	negative selection	11247976
9136528	9136551	5	-	28162770	OCIAML5	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	1.057923263403408	[123]	[465]	8	hSpCas9	negative selection	11247977
9108202	9108225	5	-	28162770	OCIAML5	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.6990055617875353	[200]	[588]	6	hSpCas9	negative selection	11247978
9066552	9066575	5	-	28162770	OCIAML5	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.636680614597856	[61]	[173]	5	hSpCas9	negative selection	11247979
9054200	9054223	5	-	28162770	OCIAML5	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.06047058788566362	[84]	[159]	0	hSpCas9	negative selection	11247980
9054139	9054162	5	+	28162770	OCIAML5	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	1.4984306762201365	[29]	[152]	9	hSpCas9	negative selection	11247981
9044516	9044539	5	+	28162770	EOL1	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.5966891315402345	[154]	[53]	-4	hSpCas9	negative selection	11417339
9226943	9226966	5	-	28162770	EOL1	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	-0.3064965200188223	[445]	[189]	-2	hSpCas9	negative selection	11417340
9197267	9197290	5	+	28162770	EOL1	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.2994352729048685	[604]	[258]	-2	hSpCas9	negative selection	11417341
9136551	9136574	5	-	28162770	EOL1	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	-0.019418193515137627	[403]	[209]	0	hSpCas9	negative selection	11417342
9136528	9136551	5	-	28162770	EOL1	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.4646286736108465	[369]	[268]	4	hSpCas9	negative selection	11417343
9108202	9108225	5	-	28162770	EOL1	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	-0.04823075969444118	[419]	[213]	0	hSpCas9	negative selection	11417344
9066552	9066575	5	-	28162770	EOL1	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.35485202750268896	[140]	[94]	3	hSpCas9	negative selection	11417345
9054200	9054223	5	-	28162770	EOL1	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	-0.5678486104145488	[165]	[58]	-4	hSpCas9	negative selection	11417346
9054139	9054162	5	+	28162770	EOL1	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.550732935018204	[91]	[70]	5	hSpCas9	negative selection	11417347
9044516	9044539	5	+	28162770	MOLM13	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-2.981418019819011	[141]	[12]	-8	hSpCas9	negative selection	11586705
9226943	9226966	5	-	28162770	MOLM13	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.975021170805408	[310]	[441]	7	hSpCas9	negative selection	11586706
9197267	9197290	5	+	28162770	MOLM13	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	0.17415817848786852	[493]	[402]	1	hSpCas9	negative selection	11586707
9136551	9136574	5	-	28162770	MOLM13	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.20485497571078498	[353]	[294]	1	hSpCas9	negative selection	11586708
9136528	9136551	5	-	28162770	MOLM13	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.9137498042903228	[300]	[409]	7	hSpCas9	negative selection	11586709
9108202	9108225	5	-	28162770	MOLM13	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.06545191904536862	[455]	[344]	0	hSpCas9	negative selection	11586710
9066552	9066575	5	-	28162770	MOLM13	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.22775537782086386	[149]	[126]	1	hSpCas9	negative selection	11586711
9054200	9054223	5	-	28162770	MOLM13	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.851630750333884	[232]	[303]	6	hSpCas9	negative selection	11586712
9054139	9054162	5	+	28162770	MOLM13	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.36209471752198197	[98]	[91]	2	hSpCas9	negative selection	11586713
9044516	9044539	5	+	28162770	HEL	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	0.29840432406471495	[145]	[114]	2	hSpCas9	negative selection	11756071
9226943	9226966	5	-	28162770	HEL	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	1.1865239260466025	[307]	[448]	9	hSpCas9	negative selection	11756072
9197267	9197290	5	+	28162770	HEL	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	0.26213482978538716	[413]	[317]	2	hSpCas9	negative selection	11756073
9136551	9136574	5	-	28162770	HEL	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.6117119363797326	[281]	[275]	5	hSpCas9	negative selection	11756074
9136528	9136551	5	-	28162770	HEL	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.008023296082101172	[294]	[189]	0	hSpCas9	negative selection	11756075
9108202	9108225	5	-	28162770	HEL	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.6469634178620822	[340]	[341]	5	hSpCas9	negative selection	11756076
9066552	9066575	5	-	28162770	HEL	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	-0.7040639315260346	[116]	[45]	-4	hSpCas9	negative selection	11756077
9054200	9054223	5	-	28162770	HEL	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.9410801065701034	[147]	[181]	7	hSpCas9	negative selection	11756078
9054139	9054162	5	+	28162770	HEL	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	1.0988035995392555	[77]	[106]	8	hSpCas9	negative selection	11756079
9044516	9044539	5	+	28162770	MV411	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-1.4973053939751502	[123]	[36]	-7	hSpCas9	negative selection	11925437
9226943	9226966	5	-	28162770	MV411	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.6624750500616192	[206]	[275]	6	hSpCas9	negative selection	11925438
9197267	9197290	5	+	28162770	MV411	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.5422695054554041	[324]	[187]	-4	hSpCas9	negative selection	11925439
9136551	9136574	5	-	28162770	MV411	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	0.5037773040425607	[215]	[257]	4	hSpCas9	negative selection	11925440
9136528	9136551	5	-	28162770	MV411	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.5651778487067042	[206]	[257]	5	hSpCas9	negative selection	11925441
9108202	9108225	5	-	28162770	MV411	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.9143934237012392	[227]	[361]	7	hSpCas9	negative selection	11925442
9066552	9066575	5	-	28162770	MV411	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.19725179361455836	[116]	[112]	1	hSpCas9	negative selection	11925443
9054200	9054223	5	-	28162770	MV411	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	1.533255352260857	[88]	[216]	9	hSpCas9	negative selection	11925444
9054139	9054162	5	+	28162770	MV411	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.5775861524751059	[69]	[87]	5	hSpCas9	negative selection	11925445
9044516	9044539	5	+	28162770	MONOMAC1	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-1.0365721748663077	[67]	[37]	-7	hSpCas9	negative selection	12094803
9226943	9226966	5	-	28162770	MONOMAC1	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.7923939112302589	[136]	[271]	7	hSpCas9	negative selection	12094804
9197267	9197290	5	+	28162770	MONOMAC1	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	0.1772001210938503	[259]	[336]	1	hSpCas9	negative selection	12094805
9136551	9136574	5	-	28162770	MONOMAC1	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	-0.37941358954812243	[159]	[140]	-3	hSpCas9	negative selection	12094806
9136528	9136551	5	-	28162770	MONOMAC1	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	1.0468887358265353	[132]	[314]	8	hSpCas9	negative selection	12094807
9108202	9108225	5	-	28162770	MONOMAC1	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.6067396646288008	[172]	[301]	6	hSpCas9	negative selection	12094808
9066552	9066575	5	-	28162770	MONOMAC1	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	1.2446686026813705	[66]	[181]	9	hSpCas9	negative selection	12094809
9054200	9054223	5	-	28162770	MONOMAC1	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	-1.1970368470595538	[83]	[41]	-7	hSpCas9	negative selection	12094810
9054139	9054162	5	+	28162770	MONOMAC1	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	-0.5545888516776374	[40]	[31]	-5	hSpCas9	negative selection	12094811
9044516	9044539	5	+	28162770	OCIAML2	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.926309309018351	[23]	[69]	-6	hSpCas9	negative selection	12264169
9226943	9226966	5	-	28162770	OCIAML2	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	1.0965433620354668	[33]	[402]	9	hSpCas9	negative selection	12264170
9197267	9197290	5	+	28162770	OCIAML2	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.7316336958485838	[70]	[236]	-5	hSpCas9	negative selection	12264171
9136551	9136574	5	-	28162770	OCIAML2	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	-0.3048209322720808	[38]	[174]	-2	hSpCas9	negative selection	12264172
9136528	9136551	5	-	28162770	OCIAML2	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.9424461577033965	[45]	[489]	8	hSpCas9	negative selection	12264173
9108202	9108225	5	-	28162770	OCIAML2	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.5419702115374725	[56]	[459]	5	hSpCas9	negative selection	12264174
9066552	9066575	5	-	28162770	OCIAML2	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	-0.25912572004844947	[18]	[87]	-2	hSpCas9	negative selection	12264175
9054200	9054223	5	-	28162770	OCIAML2	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.1748742174140457	[34]	[218]	1	hSpCas9	negative selection	12264176
9054139	9054162	5	+	28162770	OCIAML2	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	0.3194471053836072	[11]	[82]	3	hSpCas9	negative selection	12264177
9044516	9044539	5	+	28162770	OCIAML3	viability	GCAGGGTGAGGAGGCAGCCGAGG	SEMA5A	ENSG00000112902	-0.41950990909202646	[34]	[78]	-3	hSpCas9	negative selection	12433535
9226943	9226966	5	-	28162770	OCIAML3	viability	GGAATGTCAGAACTACATCCGGG	SEMA5A	ENSG00000112902	0.15015345524624704	[59]	[200]	1	hSpCas9	negative selection	12433536
9197267	9197290	5	+	28162770	OCIAML3	viability	GTTGAAGACGCACACAGCTGAGG	SEMA5A	ENSG00000112902	-0.9887251552234111	[93]	[142]	-6	hSpCas9	negative selection	12433537
9136551	9136574	5	-	28162770	OCIAML3	viability	GAAGAAATGCACAAGCCTGGAGG	SEMA5A	ENSG00000112902	1.055806004746961	[57]	[363]	8	hSpCas9	negative selection	12433538
9136528	9136551	5	-	28162770	OCIAML3	viability	AGAGCCTGAGCATGACGCAGTGG	SEMA5A	ENSG00000112902	0.8794082077488354	[55]	[310]	7	hSpCas9	negative selection	12433539
9108202	9108225	5	-	28162770	OCIAML3	viability	ACGGTGCACAGCCCAATGCGGGG	SEMA5A	ENSG00000112902	0.6818029877985372	[88]	[430]	6	hSpCas9	negative selection	12433540
9066552	9066575	5	-	28162770	OCIAML3	viability	GTCAACATCTCTGACAACGGCGG	SEMA5A	ENSG00000112902	0.20668355145243034	[30]	[107]	2	hSpCas9	negative selection	12433541
9054200	9054223	5	-	28162770	OCIAML3	viability	AAATGTTCAGCAACATGCGGCGG	SEMA5A	ENSG00000112902	0.559466840337555	[48]	[217]	5	hSpCas9	negative selection	12433542
9054139	9054162	5	+	28162770	OCIAML3	viability	GATGTCCCCTCCATAGGCCGGGG	SEMA5A	ENSG00000112902	-0.13457602168686567	[11]	[32]	-1	hSpCas9	negative selection	12433543