Crispr

Gene Info

• Species:Human (Homo sapiens)
• GeneID:6674
• Symbol:Spag1
• Description:sperm associated antigen 1

DataSource: http://genomecrispr.dkfz.de/#!/results/Spag1

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Start	The end	chr	strand	pubmed	cellline	condition	sequence	symbol	ensg	log2fc	rc_initial	rc_final	effect	cas	screentype	index
100240420	100240443	8	+	26472758	Jiyoye	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.5210339354261162	[195]	[102]	-4	hSpCas9	negative selection	154101
100194205	100194228	8	+	26472758	Jiyoye	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	-1.3704321971576787	[23]	[6]	-7	hSpCas9	negative selection	154102
100194157	100194180	8	+	26472758	Jiyoye	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.5564013902803495	[155]	[172]	4	hSpCas9	negative selection	154103
100194238	100194261	8	+	26472758	Jiyoye	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	-0.34771212065759505	[53]	[31]	-2	hSpCas9	negative selection	154104
100213234	100213257	8	+	26472758	Jiyoye	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.48063754344689436	[133]	[140]	3	hSpCas9	negative selection	154105
100213361	100213384	8	+	26472758	Jiyoye	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	1.6702097873396673	[19]	[47]	8	hSpCas9	negative selection	154106
100213863	100213886	8	+	26472758	Jiyoye	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-1.0249356311319118	[169]	[62]	-6	hSpCas9	negative selection	154107
100239302	100239325	8	+	26472758	Jiyoye	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	-1.0031085878023358	[100]	[37]	-6	hSpCas9	negative selection	154108
100240399	100240422	8	+	26472758	Jiyoye	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.38060400221496987	[182]	[105]	-3	hSpCas9	negative selection	154109
100240672	100240695	8	-	26472758	Jiyoye	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	-0.06675580682653892	[249]	[179]	0	hSpCas9	negative selection	154110
100240420	100240443	8	+	26472758	KBM7	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-1.1748152335699884	[495,326]	[49,114]	-7	hSpCas9	negative selection	345219
100194205	100194228	8	+	26472758	KBM7	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	-0.01943962528936538	[98,124]	[74,32]	0	hSpCas9	negative selection	345220
100194157	100194180	8	+	26472758	KBM7	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.9641098905744231	[454,440]	[491,340]	9	hSpCas9	negative selection	345221
100194238	100194261	8	+	26472758	KBM7	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.14413417127962092	[226,113]	[113,60]	1	hSpCas9	negative selection	345222
100213234	100213257	8	+	26472758	KBM7	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.8807485275691072	[570,316]	[383,366]	8	hSpCas9	negative selection	345223
100213361	100213384	8	+	26472758	KBM7	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	0.6617217787092071	[161,36]	[57,79]	7	hSpCas9	negative selection	345224
100213863	100213886	8	+	26472758	KBM7	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.11801754589644792	[638,751]	[327,284]	-1	hSpCas9	negative selection	345225
100239302	100239325	8	+	26472758	KBM7	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.1998255301095168	[464,370]	[181,261]	2	hSpCas9	negative selection	345226
100240399	100240422	8	+	26472758	KBM7	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.48240165508313015	[658,736]	[484,443]	5	hSpCas9	negative selection	345227
100240672	100240695	8	-	26472758	KBM7	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	-0.45174367538251586	[900,841]	[308,291]	-4	hSpCas9	negative selection	345228
100240420	100240443	8	+	26472758	Raji	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	0.21608979481609947	[114]	[30]	2	hSpCas9	negative selection	536337
100194205	100194228	8	+	26472758	Raji	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	1.8760579892671436	[26]	[22]	9	hSpCas9	negative selection	536338
100194157	100194180	8	+	26472758	Raji	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.8301552479570514	[285]	[117]	7	hSpCas9	negative selection	536339
100194238	100194261	8	+	26472758	Raji	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.9229589642361719	[74]	[32]	8	hSpCas9	negative selection	536340
100213234	100213257	8	+	26472758	Raji	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	1.5036844720708573	[154]	[101]	9	hSpCas9	negative selection	536341
100213361	100213384	8	+	26472758	Raji	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.10021188403476788	[96]	[20]	-1	hSpCas9	negative selection	536342
100213863	100213886	8	+	26472758	Raji	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.31923921728183025	[372]	[107]	3	hSpCas9	negative selection	536343
100239302	100239325	8	+	26472758	Raji	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.930885880007801	[164]	[72]	8	hSpCas9	negative selection	536344
100240399	100240422	8	+	26472758	Raji	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.34733432809435216	[314]	[92]	3	hSpCas9	negative selection	536345
100240672	100240695	8	-	26472758	Raji	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	-0.3023401640087766	[541]	[101]	-3	hSpCas9	negative selection	536346
100240420	100240443	8	+	26472758	K562	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-3.014967901152203	[315]	[32]	-8	hSpCas9	negative selection	727455
100194205	100194228	8	+	26472758	K562	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.06221539644569796	[117]	[103]	0	hSpCas9	negative selection	727456
100194157	100194180	8	+	26472758	K562	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.31153292352498363	[482]	[505]	2	hSpCas9	negative selection	727457
100194238	100194261	8	+	26472758	K562	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.2331034144386126	[127]	[126]	1	hSpCas9	negative selection	727458
100213234	100213257	8	+	26472758	K562	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.9351758924101998	[367]	[593]	7	hSpCas9	negative selection	727459
100213361	100213384	8	+	26472758	K562	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	0.6477744218976553	[92]	[122]	5	hSpCas9	negative selection	727460
100213863	100213886	8	+	26472758	K562	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.1681961093455412	[594]	[446]	-1	hSpCas9	negative selection	727461
100239302	100239325	8	+	26472758	K562	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.28177214214881646	[304]	[312]	2	hSpCas9	negative selection	727462
100240399	100240422	8	+	26472758	K562	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.31695727522492834	[542]	[570]	2	hSpCas9	negative selection	727463
100240672	100240695	8	-	26472758	K562	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.7240282282413744	[638]	[890]	6	hSpCas9	negative selection	727464
100177920	100177943	8	+	26627737	DLD1	viability	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.45694192361932207	[825]	[429,276,352]	5	hSpCas9	negative selection	837724
100183409	100183432	8	+	26627737	DLD1	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.000708495505403306	[1266]	[393,509,303]	0	hSpCas9	negative selection	837725
100187217	100187240	8	+	26627737	DLD1	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.8923118312811863	[174]	[59,135,108]	8	hSpCas9	negative selection	837726
100191475	100191498	8	+	26627737	DLD1	viability	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	0.2920704288497971	[339]	[110,101,169]	3	hSpCas9	negative selection	837727
100213348	100213371	8	+	26627737	DLD1	viability	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.23239367970736446	[1189]	[213,355,372]	-2	hSpCas9	negative selection	837728
100220302	100220325	8	+	26627737	DLD1	viability	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.6991178982491573	[332]	[152,227,133]	7	hSpCas9	negative selection	837729
100177920	100177943	8	+	26627737	GBM cells	viability after 5 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.3616704432336709	[393]	[268,273]	5	hSpCas9	negative selection	920039
100183409	100183432	8	+	26627737	GBM cells	viability after 5 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.4817247047517932	[670]	[547,456]	7	hSpCas9	negative selection	920040
100187217	100187240	8	+	26627737	GBM cells	viability after 5 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.39952586707930693	[197]	[71,88]	-6	hSpCas9	negative selection	920041
100191475	100191498	8	+	26627737	GBM cells	viability after 5 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.18354828530628753	[125]	[67,50]	-3	hSpCas9	negative selection	920042
100213348	100213371	8	+	26627737	GBM cells	viability after 5 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.3117651258869717	[551]	[221,254]	-5	hSpCas9	negative selection	920043
100220302	100220325	8	+	26627737	GBM cells	viability after 5 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.06978999462477581	[227]	[109,122]	-1	hSpCas9	negative selection	920044
100177920	100177943	8	+	26627737	GBM cells	viability after 13 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.35878009813995015	[393]	[336,222]	4	hSpCas9	negative selection	1002354
100183409	100183432	8	+	26627737	GBM cells	viability after 13 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.5073287209685564	[670]	[613,438]	6	hSpCas9	negative selection	1002355
100187217	100187240	8	+	26627737	GBM cells	viability after 13 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.519283699846125	[197]	[53,91]	-6	hSpCas9	negative selection	1002356
100191475	100191498	8	+	26627737	GBM cells	viability after 13 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.32913514015052914	[125]	[65,44]	-4	hSpCas9	negative selection	1002357
100213348	100213371	8	+	26627737	GBM cells	viability after 13 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.3364700779632178	[551]	[218,251]	-4	hSpCas9	negative selection	1002358
100220302	100220325	8	+	26627737	GBM cells	viability after 13 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.4576378368423113	[227]	[115,68]	-5	hSpCas9	negative selection	1002359
100177920	100177943	8	+	26627737	GBM cells	viability after 21 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	1.2800251420926685	[393]	[535,504]	9	hSpCas9	negative selection	1084669
100183409	100183432	8	+	26627737	GBM cells	viability after 21 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.38309615493410387	[670]	[500,451]	4	hSpCas9	negative selection	1084670
100187217	100187240	8	+	26627737	GBM cells	viability after 21 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.13326893440780174	[197]	[107,88]	-1	hSpCas9	negative selection	1084671
100191475	100191498	8	+	26627737	GBM cells	viability after 21 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-1.4385177513960847	[125]	[30,19]	-8	hSpCas9	negative selection	1084672
100213348	100213371	8	+	26627737	GBM cells	viability after 21 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.3921706508783013	[551]	[239,217]	-4	hSpCas9	negative selection	1084673
100220302	100220325	8	+	26627737	GBM cells	viability after 21 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.10396423156636408	[227]	[120,109]	-1	hSpCas9	negative selection	1084674
100177920	100177943	8	+	26627737	RPE1	viability after 9 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.9206225804406674	[1158]	[547,865]	6	hSpCas9	negative selection	1166984
100183409	100183432	8	+	26627737	RPE1	viability after 9 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	-0.23997072558203847	[3096]	[789,860]	-2	hSpCas9	negative selection	1166985
100187217	100187240	8	+	26627737	RPE1	viability after 9 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.1881007606846512	[319]	[205,256]	7	hSpCas9	negative selection	1166986
100191475	100191498	8	+	26627737	RPE1	viability after 9 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.24510963832786278	[774]	[188,224]	-2	hSpCas9	negative selection	1166987
100213348	100213371	8	+	26627737	RPE1	viability after 9 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	1.2400441305007022	[1107]	[714,953]	7	hSpCas9	negative selection	1166988
100220302	100220325	8	+	26627737	RPE1	viability after 9 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.7059978816643906	[558]	[91,126]	-5	hSpCas9	negative selection	1166989
100177920	100177943	8	+	26627737	RPE1	viability after 12 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.7429025554826909	[1158]	[490,552]	5	hSpCas9	negative selection	1249299
100183409	100183432	8	+	26627737	RPE1	viability after 12 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.14758682354035146	[3096]	[859,986]	1	hSpCas9	negative selection	1249300
100187217	100187240	8	+	26627737	RPE1	viability after 12 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.149452421303088	[319]	[146,236]	7	hSpCas9	negative selection	1249301
100191475	100191498	8	+	26627737	RPE1	viability after 12 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.1323024430936159	[774]	[171,208]	-1	hSpCas9	negative selection	1249302
100213348	100213371	8	+	26627737	RPE1	viability after 12 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	1.0217290959014165	[1107]	[379,841]	6	hSpCas9	negative selection	1249303
100220302	100220325	8	+	26627737	RPE1	viability after 12 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.5541322059634	[558]	[60,145]	-4	hSpCas9	negative selection	1249304
100177920	100177943	8	+	26627737	RPE1	viability after 15 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.7299527388983982	[1158]	[471,612]	4	hSpCas9	negative selection	1331614
100183409	100183432	8	+	26627737	RPE1	viability after 15 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.0941147300404972	[3096]	[1180,697]	0	hSpCas9	negative selection	1331615
100187217	100187240	8	+	26627737	RPE1	viability after 15 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.1785045573123454	[319]	[154,252]	6	hSpCas9	negative selection	1331616
100191475	100191498	8	+	26627737	RPE1	viability after 15 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.27238797330175424	[774]	[208,154]	-2	hSpCas9	negative selection	1331617
100213348	100213371	8	+	26627737	RPE1	viability after 15 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	1.298822347746722	[1107]	[707,831]	7	hSpCas9	negative selection	1331618
100220302	100220325	8	+	26627737	RPE1	viability after 15 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.65172953655803	[558]	[63,135]	-4	hSpCas9	negative selection	1331619
100177920	100177943	8	+	26627737	RPE1	viability after 18 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	1.10023459055015	[1158]	[584,761]	6	hSpCas9	negative selection	1413929
100183409	100183432	8	+	26627737	RPE1	viability after 18 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.059808378964476105	[3096]	[904,834]	0	hSpCas9	negative selection	1413930
100187217	100187240	8	+	26627737	RPE1	viability after 18 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.3229441797496961	[319]	[231,198]	7	hSpCas9	negative selection	1413931
100191475	100191498	8	+	26627737	RPE1	viability after 18 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.4046467673255542	[774]	[186,126]	-3	hSpCas9	negative selection	1413932
100213348	100213371	8	+	26627737	RPE1	viability after 18 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	1.2234990234765997	[1107]	[471,939]	7	hSpCas9	negative selection	1413933
100220302	100220325	8	+	26627737	RPE1	viability after 18 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.8818208271650418	[558]	[77,85]	-5	hSpCas9	negative selection	1413934
100177920	100177943	8	+	26627737	HeLa	viability after 8 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.0678221064702107	[975]	[341,618,357]	0	hSpCas9	negative selection	1496244
100183409	100183432	8	+	26627737	HeLa	viability after 8 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	-0.10097458211414989	[1104]	[435,557,354]	-1	hSpCas9	negative selection	1496245
100187217	100187240	8	+	26627737	HeLa	viability after 8 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.25178027313293083	[155]	[127,60,64]	2	hSpCas9	negative selection	1496246
100191475	100191498	8	+	26627737	HeLa	viability after 8 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	0.28752836509650526	[140]	[111,92,30]	2	hSpCas9	negative selection	1496247
100213348	100213371	8	+	26627737	HeLa	viability after 8 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.41203997034836237	[866]	[442,296,154]	-4	hSpCas9	negative selection	1496248
100220302	100220325	8	+	26627737	HeLa	viability after 8 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.031189901555682242	[302]	[216,127,65]	0	hSpCas9	negative selection	1496249
100177920	100177943	8	+	26627737	HeLa	viability after 12 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.281734184989725	[975]	[312,367,309]	-2	hSpCas9	negative selection	1578559
100183409	100183432	8	+	26627737	HeLa	viability after 12 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	-0.5238057712033637	[1104]	[121,441,369]	-5	hSpCas9	negative selection	1578560
100187217	100187240	8	+	26627737	HeLa	viability after 12 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.8678156513629295	[155]	[162,72,122]	6	hSpCas9	negative selection	1578561
100191475	100191498	8	+	26627737	HeLa	viability after 12 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	1.481477065749739	[140]	[224,114,154]	8	hSpCas9	negative selection	1578562
100213348	100213371	8	+	26627737	HeLa	viability after 12 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.6197193102267634	[866]	[100,135,468]	-5	hSpCas9	negative selection	1578563
100220302	100220325	8	+	26627737	HeLa	viability after 12 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.17436071082640686	[302]	[127,117,179]	1	hSpCas9	negative selection	1578564
100177920	100177943	8	+	26627737	HeLa	viability after 15 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.25121539561615314	[975]	[269,585,572]	2	hSpCas9	negative selection	1660874
100183409	100183432	8	+	26627737	HeLa	viability after 15 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.27396374935531354	[1104]	[533,588,505]	2	hSpCas9	negative selection	1660875
100187217	100187240	8	+	26627737	HeLa	viability after 15 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.6406758395487572	[155]	[90,132,70]	5	hSpCas9	negative selection	1660876
100191475	100191498	8	+	26627737	HeLa	viability after 15 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	1.324491184702895	[140]	[121,87,227]	8	hSpCas9	negative selection	1660877
100213348	100213371	8	+	26627737	HeLa	viability after 15 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.3738050188701083	[866]	[464,279,640]	3	hSpCas9	negative selection	1660878
100220302	100220325	8	+	26627737	HeLa	viability after 15 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.37718955729253495	[302]	[161,201,112]	3	hSpCas9	negative selection	1660879
100177920	100177943	8	+	26627737	HeLa	viability after 18 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.23173155458350148	[975]	[168,484,572]	2	hSpCas9	negative selection	1743189
100183409	100183432	8	+	26627737	HeLa	viability after 18 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.26237817135346764	[1104]	[355,479,505]	2	hSpCas9	negative selection	1743190
100187217	100187240	8	+	26627737	HeLa	viability after 18 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.652908945394798	[155]	[57,115,70]	5	hSpCas9	negative selection	1743191
100191475	100191498	8	+	26627737	HeLa	viability after 18 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	1.338093378588918	[140]	[77,80,227]	8	hSpCas9	negative selection	1743192
100213348	100213371	8	+	26627737	HeLa	viability after 18 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.36097284091074466	[866]	[307,226,640]	3	hSpCas9	negative selection	1743193
100220302	100220325	8	+	26627737	HeLa	viability after 18 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.342965576031352	[302]	[103,163,112]	3	hSpCas9	negative selection	1743194
100177920	100177943	8	+	26627737	HCT116	viability after 6 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.7363284239219503	[1552]	[320,453]	4	hSpCas9	negative selection	1825504
100183409	100183432	8	+	26627737	HCT116	viability after 6 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	1.3065780846408517	[975]	[151,504]	7	hSpCas9	negative selection	1825505
100187217	100187240	8	+	26627737	HCT116	viability after 6 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.7248006572594738	[464]	[118,2]	-4	hSpCas9	negative selection	1825506
100191475	100191498	8	+	26627737	HCT116	viability after 6 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	1.39918240889352	[390]	[87,202]	7	hSpCas9	negative selection	1825507
100213348	100213371	8	+	26627737	HCT116	viability after 6 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	1.4220478068970297	[851]	[200,445]	7	hSpCas9	negative selection	1825508
100220302	100220325	8	+	26627737	HCT116	viability after 6 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.9622340036977373	[672]	[101,27]	-5	hSpCas9	negative selection	1825509
100177920	100177943	8	+	26627737	HCT116	viability after 8 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.2552107429370579	[525]	[314,439,282]	3	hSpCas9	negative selection	1907819
100183409	100183432	8	+	26627737	HCT116	viability after 8 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.051034161359380326	[726]	[425,382,435]	0	hSpCas9	negative selection	1907820
100187217	100187240	8	+	26627737	HCT116	viability after 8 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.2289575191871116	[70]	[170,52,52]	8	hSpCas9	negative selection	1907821
100191475	100191498	8	+	26627737	HCT116	viability after 8 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	1.9612551828412028	[31]	[104,62,38]	9	hSpCas9	negative selection	1907822
100213348	100213371	8	+	26627737	HCT116	viability after 8 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.07366883165784088	[719]	[437,394,299]	-1	hSpCas9	negative selection	1907823
100220302	100220325	8	+	26627737	HCT116	viability after 8 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.358055890221006	[310]	[241,252,164]	4	hSpCas9	negative selection	1907824
100177920	100177943	8	+	26627737	HCT116	viability after 9 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.9651144426980981	[1552]	[155,256]	-6	hSpCas9	negative selection	1990134
100183409	100183432	8	+	26627737	HCT116	viability after 9 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.37120432085941757	[975]	[321,348]	2	hSpCas9	negative selection	1990135
100187217	100187240	8	+	26627737	HCT116	viability after 9 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.09183738242177697	[464]	[133,130]	0	hSpCas9	negative selection	1990136
100191475	100191498	8	+	26627737	HCT116	viability after 9 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	0.48165145465277004	[390]	[185,113]	3	hSpCas9	negative selection	1990137
100213348	100213371	8	+	26627737	HCT116	viability after 9 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.3729555755808256	[851]	[531,106]	2	hSpCas9	negative selection	1990138
100220302	100220325	8	+	26627737	HCT116	viability after 9 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.49741490438565283	[672]	[195,72]	-4	hSpCas9	negative selection	1990139
100177920	100177943	8	+	26627737	HCT116	viability after 12 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.7026210759540225	[525]	[455,363,526]	6	hSpCas9	negative selection	2072449
100183409	100183432	8	+	26627737	HCT116	viability after 12 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	-0.1632563618771632	[726]	[401,332,282]	-2	hSpCas9	negative selection	2072450
100187217	100187240	8	+	26627737	HCT116	viability after 12 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.1191364503457857	[70]	[57,73,112]	8	hSpCas9	negative selection	2072451
100191475	100191498	8	+	26627737	HCT116	viability after 12 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	3.0486106464970475	[31]	[92,118,208]	9	hSpCas9	negative selection	2072452
100213348	100213371	8	+	26627737	HCT116	viability after 12 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.18933680129306407	[719]	[189,414,404]	-2	hSpCas9	negative selection	2072453
100220302	100220325	8	+	26627737	HCT116	viability after 12 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.3736390084475086	[310]	[123,212,305]	4	hSpCas9	negative selection	2072454
100177920	100177943	8	+	26627737	HCT116	viability after 15 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.44880972506643524	[525]	[359,410,458]	4	hSpCas9	negative selection	2154764
100183409	100183432	8	+	26627737	HCT116	viability after 15 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.011521141592173656	[726]	[598,325,359]	0	hSpCas9	negative selection	2154765
100187217	100187240	8	+	26627737	HCT116	viability after 15 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.2635298482177708	[70]	[132,70,93]	8	hSpCas9	negative selection	2154766
100191475	100191498	8	+	26627737	HCT116	viability after 15 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	2.7981218446629796	[31]	[158,161,66]	9	hSpCas9	negative selection	2154767
100213348	100213371	8	+	26627737	HCT116	viability after 15 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.015671519159709657	[719]	[403,374,446]	0	hSpCas9	negative selection	2154768
100220302	100220325	8	+	26627737	HCT116	viability after 15 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.41942616472698385	[310]	[112,325,261]	4	hSpCas9	negative selection	2154769
100177920	100177943	8	+	26627737	HCT116	viability after 18 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.2921412378565752	[525]	[302,195,448]	2	hSpCas9	negative selection	2237079
100183409	100183432	8	+	26627737	HCT116	viability after 18 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	-0.34832487145825697	[726]	[275,260,292]	-3	hSpCas9	negative selection	2237080
100187217	100187240	8	+	26627737	HCT116	viability after 18 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.2732910063478147	[70]	[114,68,64]	8	hSpCas9	negative selection	2237081
100191475	100191498	8	+	26627737	HCT116	viability after 18 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	2.436962361641303	[31]	[61,103,83]	9	hSpCas9	negative selection	2237082
100213348	100213371	8	+	26627737	HCT116	viability after 18 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.013104045792196262	[719]	[329,280,431]	0	hSpCas9	negative selection	2237083
100220302	100220325	8	+	26627737	HCT116	viability after 18 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.16237255866859512	[310]	[67,161,172]	-1	hSpCas9	negative selection	2237084
100231930	100231953	8	-	25494202	A375	resistance to PLX-4720 (puromycin)	TCTTGCTCTTTCACCCAGGCCAG	SPAG1	ENSG00000104450	-3.4752685797456295	[670,81]	[9,2]	-9	dCas9-VP64	positive selection	2390680
100166532	100166555	8	+	25494202	A375	resistance to PLX-4720 (puromycin)	CAGGTGTGAGCCACCATGCCTGG	SPAG1	ENSG00000104450	-2.540049325425791	[4,100]	[0,1]	-9	dCas9-VP64	positive selection	2419215
100216977	100217000	8	-	25494202	A375	resistance to PLX-4720 (puromycin)	GCGCCACTGCACTCCAGCCTCGG	SPAG1	ENSG00000104450	0.12883397399525423	[86,65]	[25,8]	0	dCas9-VP64	positive selection	2424654
100158140	100158163	8	-	25494202	A375	resistance to PLX-4720 (puromycin)	ACAAGTCCTATATTCCCGGCCGG	SPAG1	ENSG00000104450	2.0289220470823226	[58,268]	[10,296]	7	dCas9-VP64	positive selection	2432092
100158236	100158259	8	+	25494202	A375	resistance to PLX-4720 (puromycin)	AGGGAGAAGGAGCGATGGGGTGG	SPAG1	ENSG00000104450	-0.4105240320082837	[1,30]	[0,2]	-2	dCas9-VP64	positive selection	2432093
100158183	100158206	8	-	25494202	A375	resistance to PLX-4720 (puromycin)	TCGACCTCTCGCCCCCGCTCCGG	SPAG1	ENSG00000104450	-1.3653378696573162	[176,187]	[12,16]	-7	dCas9-VP64	positive selection	2432094
100216959	100216982	8	+	25494202	A375	resistance to PLX-4720 (puromycin)	CTGAGTCTTGCTCTGTCCCCGAG	SPAG1	ENSG00000104450	1.078576075408661	[44,32]	[25,9]	4	dCas9-VP64	positive selection	2434422
100192293	100192316	8	-	25494202	A375	resistance to PLX-4720 (puromycin)	GGAGTCTCACTCTGTTGCCCCAG	SPAG1	ENSG00000104450	-0.8039407674710506	[25,109]	[4,11]	-4	dCas9-VP64	positive selection	2453851
100193654	100193677	8	+	25494202	A375	resistance to PLX-4720 (puromycin)	CGCCTGTAATCCCAGAACTTCAG	SPAG1	ENSG00000104450	-2.543268417376507	[115,242]	[7,4]	-9	dCas9-VP64	positive selection	2456182
100157910	100157933	8	+	25494202	A375	resistance to PLX-4720 (puromycin)	AGGCGGGGAAGGTGAACCTGTGG	SPAG1	ENSG00000104450	5.587202978679101	[48,74]	[10,1361]	9	dCas9-VP64	positive selection	2470147
100231930	100231953	8	-	25494202	A375	resistance to PLX-4720 (zeocin)	TCTTGCTCTTTCACCCAGGCCAG	SPAG1	ENSG00000104450	0.8980088860596516	[158,118]	[140,31]	6	dCas9-VP64	positive selection	2485935
100166532	100166555	8	+	25494202	A375	resistance to PLX-4720 (zeocin)	CAGGTGTGAGCCACCATGCCTGG	SPAG1	ENSG00000104450	0.03661325214909955	[138,24]	[31,22]	0	dCas9-VP64	positive selection	2514470
100216977	100217000	8	-	25494202	A375	resistance to PLX-4720 (zeocin)	GCGCCACTGCACTCCAGCCTCGG	SPAG1	ENSG00000104450	-0.30672599620467045	[261,149]	[50,57]	-3	dCas9-VP64	positive selection	2519909
100158140	100158163	8	-	25494202	A375	resistance to PLX-4720 (zeocin)	ACAAGTCCTATATTCCCGGCCGG	SPAG1	ENSG00000104450	-0.0793700565163924	[322,140]	[99,45]	0	dCas9-VP64	positive selection	2527347
100158236	100158259	8	+	25494202	A375	resistance to PLX-4720 (zeocin)	AGGGAGAAGGAGCGATGGGGTGG	SPAG1	ENSG00000104450	2.576295841269297	[102,75]	[284,72]	9	dCas9-VP64	positive selection	2527348
100158183	100158206	8	-	25494202	A375	resistance to PLX-4720 (zeocin)	TCGACCTCTCGCCCCCGCTCCGG	SPAG1	ENSG00000104450	0.8862625370947451	[238,152]	[28,202]	6	dCas9-VP64	positive selection	2527349
100216959	100216982	8	+	25494202	A375	resistance to PLX-4720 (zeocin)	CTGAGTCTTGCTCTGTCCCCGAG	SPAG1	ENSG00000104450	-1.053186953632317	[391,79]	[49,24]	-8	dCas9-VP64	positive selection	2529677
100192293	100192316	8	-	25494202	A375	resistance to PLX-4720 (zeocin)	GGAGTCTCACTCTGTTGCCCCAG	SPAG1	ENSG00000104450	-0.1109200555519485	[37,10]	[8,4]	-1	dCas9-VP64	positive selection	2549106
100193654	100193677	8	+	25494202	A375	resistance to PLX-4720 (zeocin)	CGCCTGTAATCCCAGAACTTCAG	SPAG1	ENSG00000104450	-0.5948923258591747	[103,174]	[39,19]	-6	dCas9-VP64	positive selection	2551437
100157910	100157933	8	+	25494202	A375	resistance to PLX-4720 (zeocin)	AGGCGGGGAAGGTGAACCTGTGG	SPAG1	ENSG00000104450	-1.0489951675912867	[64,272]	[27,23]	-8	dCas9-VP64	positive selection	2565402
100213378	100213401	8	-	27260157	DLD1	viability	CGAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.218319644842881	[]	[]	3	hSpCas9	negative selection	2579524
100233508	100233531	8	+	27260157	DLD1	viability	AGACGAGCTCTGGCTCATAAAGG	SPAG1	ENSG00000104450	0.425813274273668	[]	[]	7	hSpCas9	negative selection	2580438
100183409	100183432	8	+	27260157	DLD1	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.343862861599725	[]	[]	4	hSpCas9	negative selection	2580847
100225308	100225331	8	+	27260157	DLD1	viability	CTCCAGCAGCCATCGCCAGCAGG	SPAG1	ENSG00000104450	1.69755072347593	[]	[]	9	hSpCas9	negative selection	2580980
100220310	100220333	8	-	27260157	DLD1	viability	GCCATCGCCCGCCTCAGAAGAGG	SPAG1	ENSG00000104450	0.876743853737257	[]	[]	9	hSpCas9	negative selection	2584801
100183411	100183434	8	-	27260157	DLD1	viability	TCCCATTCCGCGTAATCCCTTGG	SPAG1	ENSG00000104450	-0.482134269799822	[]	[]	-4	hSpCas9	negative selection	2585662
100213379	100213402	8	+	27260157	DLD1	viability	CGAGGCGGCCGGCAAGTACTCGG	SPAG1	ENSG00000104450	0.880481251899439	[]	[]	9	hSpCas9	negative selection	2604106
100225255	100225278	8	-	27260157	DLD1	viability	GCCGGATGCCAAGCTTGCAGTGG	SPAG1	ENSG00000104450	0.179938224024785	[]	[]	3	hSpCas9	negative selection	2605017
100213397	100213420	8	+	27260157	DLD1	viability	CTCGGCGGCAATCGCGCTCCTGG	SPAG1	ENSG00000104450	0.177376733862975	[]	[]	3	hSpCas9	negative selection	2605961
100213361	100213384	8	-	27260157	DLD1	viability	CCTCGGCGAACTGCCCGCTTCGG	SPAG1	ENSG00000104450	0.339052687654242	[]	[]	4	hSpCas9	negative selection	2608344
100213347	100213370	8	+	27260157	DLD1	viability	GGCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.36967820128386	[]	[]	4	hSpCas9	negative selection	2609963
100187217	100187240	8	+	27260157	DLD1	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.0452328614497435	[]	[]	0	hSpCas9	negative selection	2610494
100213311	100213334	8	+	27260157	DLD1	viability	CAGGGCGCGGAGAACCCTGCCGG	SPAG1	ENSG00000104450	-0.877899780795436	[]	[]	-6	hSpCas9	negative selection	2611848
100225204	100225227	8	-	27260157	DLD1	viability	GACAGCTTCTCCCGCCAATTTGG	SPAG1	ENSG00000104450	0.621097678004338	[]	[]	8	hSpCas9	negative selection	2612939
100213120	100213143	8	-	27260157	DLD1	viability	GCCCATGACGCACGGCTGGGCGG	SPAG1	ENSG00000104450	0.587882453025333	[]	[]	8	hSpCas9	negative selection	2613553
100213165	100213188	8	+	27260157	DLD1	viability	GCAAAGCCGAAGGCGGCAAGCGG	SPAG1	ENSG00000104450	-0.673765137657245	[]	[]	-5	hSpCas9	negative selection	2614123
100213171	100213194	8	-	27260157	DLD1	viability	TGCCGGCCGCTTGCCGCCTTCGG	SPAG1	ENSG00000104450	0.434003888713395	[]	[]	7	hSpCas9	negative selection	2623401
100213343	100213366	8	-	27260157	DLD1	viability	TTCGGAACAGCTCGTTGCCCTGG	SPAG1	ENSG00000104450	0.10507369491653	[]	[]	3	hSpCas9	negative selection	2641928
100233500	100233523	8	-	27260157	DLD1	viability	AGCCAGAGCTCGTCTATAGAAGG	SPAG1	ENSG00000104450	-0.165606107809062	[]	[]	-1	hSpCas9	negative selection	2644521
100233466	100233489	8	+	27260157	DLD1	viability	CAGGCACTTCAGCTAGCTGATGG	SPAG1	ENSG00000104450	-0.0433344418783918	[]	[]	0	hSpCas9	negative selection	2647428
100213378	100213401	8	-	27260157	HT1080	viability	CGAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.682298622076058	[]	[]	6	hSpCas9	negative selection	2655654
100233508	100233531	8	+	27260157	HT1080	viability	AGACGAGCTCTGGCTCATAAAGG	SPAG1	ENSG00000104450	0.0796538111385287	[]	[]	0	hSpCas9	negative selection	2656568
100183409	100183432	8	+	27260157	HT1080	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	1.2239825487001	[]	[]	8	hSpCas9	negative selection	2656977
100225308	100225331	8	+	27260157	HT1080	viability	CTCCAGCAGCCATCGCCAGCAGG	SPAG1	ENSG00000104450	1.34789177189198	[]	[]	9	hSpCas9	negative selection	2657110
100220310	100220333	8	-	27260157	HT1080	viability	GCCATCGCCCGCCTCAGAAGAGG	SPAG1	ENSG00000104450	1.00561542793952	[]	[]	8	hSpCas9	negative selection	2660931
100183411	100183434	8	-	27260157	HT1080	viability	TCCCATTCCGCGTAATCCCTTGG	SPAG1	ENSG00000104450	-0.946936718468362	[]	[]	-8	hSpCas9	negative selection	2661792
100213379	100213402	8	+	27260157	HT1080	viability	CGAGGCGGCCGGCAAGTACTCGG	SPAG1	ENSG00000104450	0.263445332271432	[]	[]	2	hSpCas9	negative selection	2680236
100225255	100225278	8	-	27260157	HT1080	viability	GCCGGATGCCAAGCTTGCAGTGG	SPAG1	ENSG00000104450	0.00768851013664499	[]	[]	0	hSpCas9	negative selection	2681147
100213397	100213420	8	+	27260157	HT1080	viability	CTCGGCGGCAATCGCGCTCCTGG	SPAG1	ENSG00000104450	-1.19092734605744	[]	[]	-8	hSpCas9	negative selection	2682091
100213361	100213384	8	-	27260157	HT1080	viability	CCTCGGCGAACTGCCCGCTTCGG	SPAG1	ENSG00000104450	-0.0418745784262828	[]	[]	0	hSpCas9	negative selection	2684474
100213347	100213370	8	+	27260157	HT1080	viability	GGCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.380921462376216	[]	[]	3	hSpCas9	negative selection	2686093
100187217	100187240	8	+	27260157	HT1080	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.346200385827025	[]	[]	3	hSpCas9	negative selection	2686624
100213311	100213334	8	+	27260157	HT1080	viability	CAGGGCGCGGAGAACCCTGCCGG	SPAG1	ENSG00000104450	-0.300893402736298	[]	[]	-7	hSpCas9	negative selection	2687978
100225204	100225227	8	-	27260157	HT1080	viability	GACAGCTTCTCCCGCCAATTTGG	SPAG1	ENSG00000104450	0.822275743790867	[]	[]	7	hSpCas9	negative selection	2689069
100213120	100213143	8	-	27260157	HT1080	viability	GCCCATGACGCACGGCTGGGCGG	SPAG1	ENSG00000104450	0.811895585626467	[]	[]	7	hSpCas9	negative selection	2689683
100213165	100213188	8	+	27260157	HT1080	viability	GCAAAGCCGAAGGCGGCAAGCGG	SPAG1	ENSG00000104450	-0.36018366323737	[]	[]	-7	hSpCas9	negative selection	2690253
100213171	100213194	8	-	27260157	HT1080	viability	TGCCGGCCGCTTGCCGCCTTCGG	SPAG1	ENSG00000104450	0.665574021137535	[]	[]	6	hSpCas9	negative selection	2699531
100213343	100213366	8	-	27260157	HT1080	viability	TTCGGAACAGCTCGTTGCCCTGG	SPAG1	ENSG00000104450	0.655786258328488	[]	[]	6	hSpCas9	negative selection	2718058
100233500	100233523	8	-	27260157	HT1080	viability	AGCCAGAGCTCGTCTATAGAAGG	SPAG1	ENSG00000104450	-0.295868193013194	[]	[]	-7	hSpCas9	negative selection	2720651
100233466	100233489	8	+	27260157	HT1080	viability	CAGGCACTTCAGCTAGCTGATGG	SPAG1	ENSG00000104450	0.167431217930935	[]	[]	1	hSpCas9	negative selection	2723558
100213378	100213401	8	-	27260157	MKN45	viability	CGAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	1.31523295153344	[]	[]	9	hSpCas9	negative selection	2731784
100233508	100233531	8	+	27260157	MKN45	viability	AGACGAGCTCTGGCTCATAAAGG	SPAG1	ENSG00000104450	0.221719096359876	[]	[]	5	hSpCas9	negative selection	2732698
100183409	100183432	8	+	27260157	MKN45	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.930757638769411	[]	[]	8	hSpCas9	negative selection	2733107
100225308	100225331	8	+	27260157	MKN45	viability	CTCCAGCAGCCATCGCCAGCAGG	SPAG1	ENSG00000104450	1.30527806562125	[]	[]	9	hSpCas9	negative selection	2733240
100220310	100220333	8	-	27260157	MKN45	viability	GCCATCGCCCGCCTCAGAAGAGG	SPAG1	ENSG00000104450	0.651931147029054	[]	[]	7	hSpCas9	negative selection	2737061
100183411	100183434	8	-	27260157	MKN45	viability	TCCCATTCCGCGTAATCCCTTGG	SPAG1	ENSG00000104450	-0.166541573414008	[]	[]	0	hSpCas9	negative selection	2737922
100213379	100213402	8	+	27260157	MKN45	viability	CGAGGCGGCCGGCAAGTACTCGG	SPAG1	ENSG00000104450	-0.270122460242142	[]	[]	-1	hSpCas9	negative selection	2756366
100225255	100225278	8	-	27260157	MKN45	viability	GCCGGATGCCAAGCTTGCAGTGG	SPAG1	ENSG00000104450	0.260243649985685	[]	[]	5	hSpCas9	negative selection	2757277
100213397	100213420	8	+	27260157	MKN45	viability	CTCGGCGGCAATCGCGCTCCTGG	SPAG1	ENSG00000104450	-1.31793420351959	[]	[]	-8	hSpCas9	negative selection	2758221
100213361	100213384	8	-	27260157	MKN45	viability	CCTCGGCGAACTGCCCGCTTCGG	SPAG1	ENSG00000104450	-0.0134615177330476	[]	[]	0	hSpCas9	negative selection	2760604
100213347	100213370	8	+	27260157	MKN45	viability	GGCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.568309206370614	[]	[]	7	hSpCas9	negative selection	2762223
100187217	100187240	8	+	27260157	MKN45	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.145542510479913	[]	[]	0	hSpCas9	negative selection	2762754
100213311	100213334	8	+	27260157	MKN45	viability	CAGGGCGCGGAGAACCCTGCCGG	SPAG1	ENSG00000104450	0.644968065157993	[]	[]	7	hSpCas9	negative selection	2764108
100225204	100225227	8	-	27260157	MKN45	viability	GACAGCTTCTCCCGCCAATTTGG	SPAG1	ENSG00000104450	0.526134665225273	[]	[]	6	hSpCas9	negative selection	2765199
100213120	100213143	8	-	27260157	MKN45	viability	GCCCATGACGCACGGCTGGGCGG	SPAG1	ENSG00000104450	0.645653248253733	[]	[]	7	hSpCas9	negative selection	2765813
100213165	100213188	8	+	27260157	MKN45	viability	GCAAAGCCGAAGGCGGCAAGCGG	SPAG1	ENSG00000104450	-1.14234403924886	[]	[]	-8	hSpCas9	negative selection	2766383
100213171	100213194	8	-	27260157	MKN45	viability	TGCCGGCCGCTTGCCGCCTTCGG	SPAG1	ENSG00000104450	-0.364900963156322	[]	[]	-1	hSpCas9	negative selection	2775661
100213343	100213366	8	-	27260157	MKN45	viability	TTCGGAACAGCTCGTTGCCCTGG	SPAG1	ENSG00000104450	0.0719650858628905	[]	[]	0	hSpCas9	negative selection	2794188
100233500	100233523	8	-	27260157	MKN45	viability	AGCCAGAGCTCGTCTATAGAAGG	SPAG1	ENSG00000104450	0.736051224853262	[]	[]	7	hSpCas9	negative selection	2796781
100233466	100233489	8	+	27260157	MKN45	viability	CAGGCACTTCAGCTAGCTGATGG	SPAG1	ENSG00000104450	0.449181490044849	[]	[]	6	hSpCas9	negative selection	2799688
100213378	100213401	8	-	27260157	RKO	viability	CGAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.150554855207896	[]	[]	0	hSpCas9	negative selection	2807914
100233508	100233531	8	+	27260157	RKO	viability	AGACGAGCTCTGGCTCATAAAGG	SPAG1	ENSG00000104450	-0.0479260851857455	[]	[]	0	hSpCas9	negative selection	2808828
100183409	100183432	8	+	27260157	RKO	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.161755437982905	[]	[]	0	hSpCas9	negative selection	2809237
100225308	100225331	8	+	27260157	RKO	viability	CTCCAGCAGCCATCGCCAGCAGG	SPAG1	ENSG00000104450	1.19328944555305	[]	[]	9	hSpCas9	negative selection	2809370
100220310	100220333	8	-	27260157	RKO	viability	GCCATCGCCCGCCTCAGAAGAGG	SPAG1	ENSG00000104450	0.0194039931090299	[]	[]	0	hSpCas9	negative selection	2813191
100183411	100183434	8	-	27260157	RKO	viability	TCCCATTCCGCGTAATCCCTTGG	SPAG1	ENSG00000104450	-1.53786552824072	[]	[]	-9	hSpCas9	negative selection	2814052
100213379	100213402	8	+	27260157	RKO	viability	CGAGGCGGCCGGCAAGTACTCGG	SPAG1	ENSG00000104450	-0.376209340742096	[]	[]	-6	hSpCas9	negative selection	2832496
100225255	100225278	8	-	27260157	RKO	viability	GCCGGATGCCAAGCTTGCAGTGG	SPAG1	ENSG00000104450	-1.19595610412094	[]	[]	-8	hSpCas9	negative selection	2833407
100213397	100213420	8	+	27260157	RKO	viability	CTCGGCGGCAATCGCGCTCCTGG	SPAG1	ENSG00000104450	-0.64411547179685	[]	[]	-7	hSpCas9	negative selection	2834351
100213361	100213384	8	-	27260157	RKO	viability	CCTCGGCGAACTGCCCGCTTCGG	SPAG1	ENSG00000104450	-0.149222393052489	[]	[]	0	hSpCas9	negative selection	2836734
100213347	100213370	8	+	27260157	RKO	viability	GGCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.0127850227378073	[]	[]	0	hSpCas9	negative selection	2838353
100187217	100187240	8	+	27260157	RKO	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.175564199252527	[]	[]	0	hSpCas9	negative selection	2838884
100213311	100213334	8	+	27260157	RKO	viability	CAGGGCGCGGAGAACCCTGCCGG	SPAG1	ENSG00000104450	-1.68854790950524	[]	[]	-9	hSpCas9	negative selection	2840238
100225204	100225227	8	-	27260157	RKO	viability	GACAGCTTCTCCCGCCAATTTGG	SPAG1	ENSG00000104450	-0.00204089434872231	[]	[]	0	hSpCas9	negative selection	2841329
100213120	100213143	8	-	27260157	RKO	viability	GCCCATGACGCACGGCTGGGCGG	SPAG1	ENSG00000104450	-0.225165703702919	[]	[]	-1	hSpCas9	negative selection	2841943
100213165	100213188	8	+	27260157	RKO	viability	GCAAAGCCGAAGGCGGCAAGCGG	SPAG1	ENSG00000104450	-0.214309589433207	[]	[]	-1	hSpCas9	negative selection	2842513
100213171	100213194	8	-	27260157	RKO	viability	TGCCGGCCGCTTGCCGCCTTCGG	SPAG1	ENSG00000104450	0.0107566036002078	[]	[]	0	hSpCas9	negative selection	2851791
100213343	100213366	8	-	27260157	RKO	viability	TTCGGAACAGCTCGTTGCCCTGG	SPAG1	ENSG00000104450	-0.740173424866024	[]	[]	-7	hSpCas9	negative selection	2870318
100233500	100233523	8	-	27260157	RKO	viability	AGCCAGAGCTCGTCTATAGAAGG	SPAG1	ENSG00000104450	-0.610328353781527	[]	[]	-7	hSpCas9	negative selection	2872911
100233466	100233489	8	+	27260157	RKO	viability	CAGGCACTTCAGCTAGCTGATGG	SPAG1	ENSG00000104450	-0.249616674169526	[]	[]	-1	hSpCas9	negative selection	2875818
100213378	100213401	8	-	27260157	SF268	viability	CGAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.916960786931023	[]	[]	9	hSpCas9	negative selection	2884044
100233508	100233531	8	+	27260157	SF268	viability	AGACGAGCTCTGGCTCATAAAGG	SPAG1	ENSG00000104450	-0.142808337681614	[]	[]	-1	hSpCas9	negative selection	2884958
100183409	100183432	8	+	27260157	SF268	viability	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.594796345715705	[]	[]	8	hSpCas9	negative selection	2885367
100225308	100225331	8	+	27260157	SF268	viability	CTCCAGCAGCCATCGCCAGCAGG	SPAG1	ENSG00000104450	1.58445739227422	[]	[]	9	hSpCas9	negative selection	2885500
100220310	100220333	8	-	27260157	SF268	viability	GCCATCGCCCGCCTCAGAAGAGG	SPAG1	ENSG00000104450	0.167530866892244	[]	[]	0	hSpCas9	negative selection	2889321
100183411	100183434	8	-	27260157	SF268	viability	TCCCATTCCGCGTAATCCCTTGG	SPAG1	ENSG00000104450	0.087006455317095	[]	[]	0	hSpCas9	negative selection	2890182
100213379	100213402	8	+	27260157	SF268	viability	CGAGGCGGCCGGCAAGTACTCGG	SPAG1	ENSG00000104450	0.167420301730153	[]	[]	0	hSpCas9	negative selection	2908626
100225255	100225278	8	-	27260157	SF268	viability	GCCGGATGCCAAGCTTGCAGTGG	SPAG1	ENSG00000104450	0.755900349972108	[]	[]	8	hSpCas9	negative selection	2909537
100213397	100213420	8	+	27260157	SF268	viability	CTCGGCGGCAATCGCGCTCCTGG	SPAG1	ENSG00000104450	-1.23703491622157	[]	[]	-7	hSpCas9	negative selection	2910481
100213361	100213384	8	-	27260157	SF268	viability	CCTCGGCGAACTGCCCGCTTCGG	SPAG1	ENSG00000104450	0.639751907246726	[]	[]	8	hSpCas9	negative selection	2912864
100213347	100213370	8	+	27260157	SF268	viability	GGCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.339069222112716	[]	[]	4	hSpCas9	negative selection	2914483
100187217	100187240	8	+	27260157	SF268	viability	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.330026125334277	[]	[]	4	hSpCas9	negative selection	2915014
100213311	100213334	8	+	27260157	SF268	viability	CAGGGCGCGGAGAACCCTGCCGG	SPAG1	ENSG00000104450	-0.383922588457574	[]	[]	-3	hSpCas9	negative selection	2916368
100225204	100225227	8	-	27260157	SF268	viability	GACAGCTTCTCCCGCCAATTTGG	SPAG1	ENSG00000104450	1.08085504684276	[]	[]	9	hSpCas9	negative selection	2917459
100213120	100213143	8	-	27260157	SF268	viability	GCCCATGACGCACGGCTGGGCGG	SPAG1	ENSG00000104450	0.670246595721508	[]	[]	8	hSpCas9	negative selection	2918073
100213165	100213188	8	+	27260157	SF268	viability	GCAAAGCCGAAGGCGGCAAGCGG	SPAG1	ENSG00000104450	0.700929836773125	[]	[]	8	hSpCas9	negative selection	2918643
100213171	100213194	8	-	27260157	SF268	viability	TGCCGGCCGCTTGCCGCCTTCGG	SPAG1	ENSG00000104450	0.302633751969381	[]	[]	4	hSpCas9	negative selection	2927921
100213343	100213366	8	-	27260157	SF268	viability	TTCGGAACAGCTCGTTGCCCTGG	SPAG1	ENSG00000104450	0.373999729092768	[]	[]	4	hSpCas9	negative selection	2946448
100233500	100233523	8	-	27260157	SF268	viability	AGCCAGAGCTCGTCTATAGAAGG	SPAG1	ENSG00000104450	-0.127901383773611	[]	[]	-1	hSpCas9	negative selection	2949041
100233466	100233489	8	+	27260157	SF268	viability	CAGGCACTTCAGCTAGCTGATGG	SPAG1	ENSG00000104450	0.649157933351898	[]	[]	8	hSpCas9	negative selection	2951948
100194159	100194182	8	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	0.4792168938202251	[3,3]	[3,5]	8	hSpCas9	positive selection	3022914
100183405	100183428	8	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	-0.1944695660840029	[3,4]	[3,2]	-3	hSpCas9	positive selection	3022915
100183990	100184013	8	+	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	0.25849996077347925	[4,4]	[5,4]	5	hSpCas9	positive selection	3022916
100213128	100213151	8	-	27453484	HT29	resistance to T3SS1-dependent cytotoxicity	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	0.19791022791765098	[3,3]	[3,3]	4	hSpCas9	positive selection	3022917
100194159	100194182	8	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	0.0915979778919434	[3,3]	[3,2]	1	hSpCas9	positive selection	3090772
100183405	100183428	8	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	0.37096728151973335	[3,3]	[2,4]	6	hSpCas9	positive selection	3090773
100183990	100184013	8	+	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	0.09688464189892493	[4,4]	[5,2]	1	hSpCas9	positive selection	3090774
100213128	100213151	8	-	27453484	HT29	resistance to T3SS2-dependent cytotoxicity	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	0.038584794248653326	[3,3]	[2,2]	0	hSpCas9	positive selection	3090775
100162399	100162422		+	24336571	A375	resistance to PLX after 7 days	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.10016828579957654	[9,9]	[9,8]	-3	hSpCas9	positive selection	3146839
100165879	100165902		+	24336571	A375	resistance to PLX after 7 days	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.41903320084276785	[7,6]	[10,9]	9	hSpCas9	positive selection	3146841
100162299	100162322		-	24336571	A375	resistance to PLX after 7 days	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	-0.01873369824243576	[7,5]	[5,7]	0	hSpCas9	positive selection	3146842
100165856	100165879		+	24336571	A375	resistance to PLX after 7 days	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	0.018907310819912038	[2,4]	[3,3]	0	hSpCas9	positive selection	3146843
100162399	100162422		+	24336571	A375	resistance to PLX after 14 days	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.2266061216062566	[7,9]	[2,3]	-2	hSpCas9	positive selection	3204632
100165879	100165902		+	24336571	A375	resistance to PLX after 14 days	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.013591296135571418	[13,4]	[3,3]	0	hSpCas9	positive selection	3204634
100162299	100162322		-	24336571	A375	resistance to PLX after 14 days	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	0.09194013968759551	[8,4]	[2,2]	1	hSpCas9	positive selection	3204635
100165856	100165879		+	24336571	A375	resistance to PLX after 14 days	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	-0.13406198712829065	[2,4]	[1,1]	-1	hSpCas9	positive selection	3204636
100162399	100162422		+	24336571	A375	viability after 7 days	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.03739307247839918	[9]	[9,9]	0	hSpCas9	negative selection	3262425
100165879	100165902		+	24336571	A375	viability after 7 days	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.04314145264392244	[7]	[7,6]	0	hSpCas9	negative selection	3262427
100162299	100162322		-	24336571	A375	viability after 7 days	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	-0.016126201352402503	[7]	[7,5]	0	hSpCas9	negative selection	3262428
100165856	100165879		+	24336571	A375	viability after 7 days	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	-0.8852700122104233	[7]	[2,4]	-8	hSpCas9	negative selection	3262429
100162399	100162422		+	24336571	A375	viability after 14 days	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.1376618911605697	[9]	[7,9]	-1	hSpCas9	negative selection	3320218
100165879	100165902		+	24336571	A375	viability after 14 days	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.3545588682050233	[7]	[13,4]	4	hSpCas9	negative selection	3320220
100162299	100162322		-	24336571	A375	viability after 14 days	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	0.044713583563393056	[7]	[8,4]	0	hSpCas9	negative selection	3320221
100165856	100165879		+	24336571	A375	viability after 14 days	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	-0.7450969865836079	[7]	[2,4]	-7	hSpCas9	negative selection	3320222
100177891	100177914	8	+	27383988	293T	resistance to West Nile virus (flavivirus)	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.5435736884744696	[222,222]	[0,0]	-4	hSpCas9	positive selection	3394436
100165836	100165859	8	-	27383988	293T	resistance to West Nile virus (flavivirus)	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.6374915518621085	[237,237]	[0,0]	-4	hSpCas9	positive selection	3394437
100165879	100165902	8	+	27383988	293T	resistance to West Nile virus (flavivirus)	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.3049162127752376	[188,188]	[0,0]	-2	hSpCas9	positive selection	3394438
100194158	100194181	8	+	26780180	HT29	viability (Avana library 4 designs)	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	0.970334237002226	[]	[]	4	hSpCas9	negative selection	3461526
100183404	100183427	8	+	26780180	HT29	viability (Avana library 4 designs)	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	-0.396543314024557	[]	[]	-6	hSpCas9	negative selection	3461527
100183989	100184012	8	+	26780180	HT29	viability (Avana library 4 designs)	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	-0.103822144839846	[]	[]	-2	hSpCas9	negative selection	3461528
100213128	100213151	8	-	26780180	HT29	viability (Avana library 4 designs)	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	1.48830952858375	[]	[]	6	hSpCas9	negative selection	3461529
100194158	100194181	8	+	26780180	A375	viability (Avana lentiCRISPRv2)	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	-0.793277279733199	[]	[]	-1	hSpCas9	negative selection	3562412
100183989	100184012	8	+	26780180	A375	viability (Avana lentiCRISPRv2)	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	-23.3164614857465	[]	[]	-9	hSpCas9	negative selection	3562413
100213128	100213151	8	-	26780180	A375	viability (Avana lentiCRISPRv2)	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	3.30386955972038	[]	[]	5	hSpCas9	negative selection	3562414
100183404	100183427	8	+	26780180	A375	viability (Avana lentiCRISPRv2)	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	-0.724561596516124	[]	[]	-1	hSpCas9	negative selection	3562415
100213076	100213099	8	+	26780180	A375	viability (Avana lentiCRISPRv2)	CCACTTCCTCACAGAGCCCGCGG	SPAG1	ENSG00000104450	-3.66216599545219	[]	[]	-4	hSpCas9	negative selection	3562416
100213326	100213349	8	+	26780180	A375	viability (Avana lentiCRISPRv2)	CCTGCCGGCCTGAAGAGCCAGGG	SPAG1	ENSG00000104450	5.11994756349982	[]	[]	8	hSpCas9	negative selection	3562417
100194158	100194181	8	+	26780180	A375	viability (Avana lentiGuide)	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	-10.0626679347858	[]	[]	-9	hSpCas9	negative selection	3671071
100183989	100184012	8	+	26780180	A375	viability (Avana lentiGuide)	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	-2.66851258043252	[]	[]	-5	hSpCas9	negative selection	3671072
100213128	100213151	8	-	26780180	A375	viability (Avana lentiGuide)	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	-1.03271193108209	[]	[]	-2	hSpCas9	negative selection	3671073
100183404	100183427	8	+	26780180	A375	viability (Avana lentiGuide)	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	-2.82987964050117	[]	[]	-5	hSpCas9	negative selection	3671074
100213076	100213099	8	+	26780180	A375	viability (Avana lentiGuide)	CCACTTCCTCACAGAGCCCGCGG	SPAG1	ENSG00000104450	10.1929902668389	[]	[]	7	hSpCas9	negative selection	3671075
100213326	100213349	8	+	26780180	A375	viability (Avana lentiGuide)	CCTGCCGGCCTGAAGAGCCAGGG	SPAG1	ENSG00000104450	6.24698860999183	[]	[]	5	hSpCas9	negative selection	3671076
100194158	100194181	8	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	-0.6536533278941501	[5]	[3,1]	-7	hSpCas9	positive selection	3779678
100183989	100184012	8	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	-0.8302953624334801	[9]	[4,4]	-8	hSpCas9	positive selection	3779679
100213128	100213151	8	-	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	0.12409681798518946	[5]	[5,5]	3	hSpCas9	positive selection	3779680
100183404	100183427	8	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	-0.14917339565522125	[5]	[5,3]	-2	hSpCas9	positive selection	3779681
100213076	100213099	8	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CCACTTCCTCACAGAGCCCGCGG	SPAG1	ENSG00000104450	0.2207121975518309	[4]	[5,4]	6	hSpCas9	positive selection	3779682
100213326	100213349	8	+	26780180	A375	resistance to vemurafenib (Avana lentiCRISPRv2)	CCTGCCGGCCTGAAGAGCCAGGG	SPAG1	ENSG00000104450	0.25340661640660067	[5]	[6,6]	7	hSpCas9	positive selection	3779683
100194158	100194181	8	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CATCTGAGACTCAAACCAAAGGG	SPAG1	ENSG00000104450	0.19851290267676955	[4]	[4,4]	5	hSpCas9	positive selection	3888285
100183989	100184012	8	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGAAGATTACAAAGAAAAGACGG	SPAG1	ENSG00000104450	0.12090618584053939	[6]	[6,5]	2	hSpCas9	positive selection	3888286
100213128	100213151	8	-	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	TGGATGTTGCCCATGACGCACGG	SPAG1	ENSG00000104450	-0.008360746607923658	[5]	[5,3]	0	hSpCas9	positive selection	3888287
100183404	100183427	8	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	GAAAACTCCAAGGGATTACGCGG	SPAG1	ENSG00000104450	0.2706323806362686	[5]	[5,6]	7	hSpCas9	positive selection	3888288
100213076	100213099	8	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CCACTTCCTCACAGAGCCCGCGG	SPAG1	ENSG00000104450	-0.3389727656818829	[5]	[3,3]	-4	hSpCas9	positive selection	3888289
100213326	100213349	8	+	26780180	A375	resistance to vemurafenib (Avana lentiGuide)	CCTGCCGGCCTGAAGAGCCAGGG	SPAG1	ENSG00000104450	0.19833561599921767	[6]	[6,6]	5	hSpCas9	positive selection	3888290
100162399	100162422	8	+	26780180	A375	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.64747641823965	[]	[]	2	hSpCas9	negative selection	3998390
100177903	100177926	8	-	26780180	A375	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.863679028790706	[]	[]	3	hSpCas9	negative selection	3998391
100162288	100162311	8	+	26780180	A375	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.813436499925721	[]	[]	3	hSpCas9	negative selection	3998392
100165879	100165902	8	+	26780180	A375	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	1.2274678027213	[]	[]	6	hSpCas9	negative selection	3998393
100177891	100177914	8	+	26780180	A375	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.501759619223978	[]	[]	1	hSpCas9	negative selection	3998394
100165836	100165859	8	-	26780180	A375	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.921702983750131	[]	[]	4	hSpCas9	negative selection	3998395
100162399	100162422	8	+	26780180	HT29	viability (GeCKOv2 library)	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	1.28399196532521	[]	[]	6	hSpCas9	negative selection	4106356
100177903	100177926	8	-	26780180	HT29	viability (GeCKOv2 library)	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	1.3598139208965	[]	[]	7	hSpCas9	negative selection	4106357
100162288	100162311	8	+	26780180	HT29	viability (GeCKOv2 library)	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.805131292672359	[]	[]	3	hSpCas9	negative selection	4106358
100165879	100165902	8	+	26780180	HT29	viability (GeCKOv2 library)	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.657561701231431	[]	[]	2	hSpCas9	negative selection	4106359
100177891	100177914	8	+	26780180	HT29	viability (GeCKOv2 library)	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.772065480087681	[]	[]	3	hSpCas9	negative selection	4106360
100165836	100165859	8	-	26780180	HT29	viability (GeCKOv2 library)	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.744361894191225	[]	[]	3	hSpCas9	negative selection	4106361
100162399	100162422	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.19586748694873	[3]	[1,1]	-4	hSpCas9	positive selection	4172975
100165879	100165902	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.10839639517868892	[3]	[2,1]	2	hSpCas9	positive selection	4172977
100162299	100162322	8	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	-0.32087891796660634	[2]	[0,1]	-7	hSpCas9	positive selection	4172978
100165856	100165879	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiCRISPRv1)	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	0.27562954139561735	[1]	[2,0]	7	hSpCas9	positive selection	4172979
100162399	100162422	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.6017136045564908	[3]	[0,0,1,3]	4	hSpCas9	positive selection	4236997
100165879	100165902	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.18964963636503335	[2]	[3,0,2,0]	1	hSpCas9	positive selection	4236999
100162299	100162322	8	-	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	GTTCCAAAGCCCCACAATGATGG	SPAG1	ENSG00000104450	-0.5941343722498258	[2]	[0,0,0,0]	-7	hSpCas9	positive selection	4237000
100165856	100165879	8	+	26780180	A375	resistance to vemurafenib (GeCKOv1 lentiGuide)	TGAAAAGCATCTTCAAGCCTTGG	SPAG1	ENSG00000104450	0.15088658279199163	[3]	[2,3,0,0]	1	hSpCas9	positive selection	4237001
100162399	100162422	8	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.2768405942328802	[1]	[0,0]	-5	hSpCas9	positive selection	4299971
100177903	100177926	8	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3715586118814854	[2]	[4,0]	7	hSpCas9	positive selection	4299972
100162288	100162311	8	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.028557200490244072	[3]	[3,2]	0	hSpCas9	positive selection	4299973
100177891	100177914	8	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.2773694331579114	[3]	[3,2]	5	hSpCas9	positive selection	4364223
100165836	100165859	8	-	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.08560858851282299	[3]	[3,2]	1	hSpCas9	positive selection	4364224
100165879	100165902	8	+	26780180	A375	resistance to vemurafenib (GeCKOv2 lentiGuide)	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.5598833041744489	[3]	[4,3]	9	hSpCas9	positive selection	4364225
100183411	100183433	8	+	27760321	OCIAML3	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	-0.05955872193134609	[468,342]	[260,354]	0	hSpCas9	negative selection	4442348
100213124	100213146	8	-	27760321	OCIAML3	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	-0.16872104193191428	[950,727]	[438,762]	-2	hSpCas9	negative selection	4442349
100213232	100213254	8	+	27760321	OCIAML3	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.07920507619766937	[577,447]	[258,530]	-1	hSpCas9	negative selection	4442350
100213348	100213370	8	+	27760321	OCIAML3	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.6893579042707262	[466,355]	[441,609]	7	hSpCas9	negative selection	4442351
100213378	100213400	8	-	27760321	OCIAML3	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.44749998965004933	[1468,1289]	[1212,1800]	5	hSpCas9	negative selection	4442352
100183411	100183433	8	+	27760321	OCIAML2	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	-0.09794868605146242	[468,342]	[306,299]	-1	hSpCas9	negative selection	4525811
100213124	100213146	8	-	27760321	OCIAML2	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.16717635668475483	[950,727]	[584,982]	2	hSpCas9	negative selection	4525812
100213232	100213254	8	+	27760321	OCIAML2	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.25111130512857277	[577,447]	[230,497]	-3	hSpCas9	negative selection	4525813
100213348	100213370	8	+	27760321	OCIAML2	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.16438073798697533	[466,355]	[367,372]	2	hSpCas9	negative selection	4525814
100213378	100213400	8	-	27760321	OCIAML2	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.5194371252676653	[1468,1289]	[1440,1800]	7	hSpCas9	negative selection	4525815
100183411	100183433	8	+	27760321	MV411	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.1719358417788366	[468,342]	[270,340]	1	hSpCas9	negative selection	4609274
100213124	100213146	8	-	27760321	MV411	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	1.2264355586165698	[950,727]	[1271,1307]	8	hSpCas9	negative selection	4609275
100213232	100213254	8	+	27760321	MV411	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.2560664165198231	[577,447]	[107,541]	-2	hSpCas9	negative selection	4609276
100213348	100213370	8	+	27760321	MV411	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	-1.2960468210548794	[466,355]	[18,245]	-7	hSpCas9	negative selection	4609277
100213378	100213400	8	-	27760321	MV411	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	1.098141067270762	[1468,1289]	[1764,2214]	7	hSpCas9	negative selection	4609278
100183411	100183433	8	+	27760321	HL60	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	1.032897623007281	[468,342]	[578,770]	9	hSpCas9	negative selection	4692737
100213124	100213146	8	-	27760321	HL60	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.4532332399297787	[950,727]	[806,1063]	5	hSpCas9	negative selection	4692738
100213232	100213254	8	+	27760321	HL60	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	0.3541487573841474	[577,447]	[393,677]	4	hSpCas9	negative selection	4692739
100213348	100213370	8	+	27760321	HL60	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	-0.6371514545989824	[466,355]	[245,180]	-6	hSpCas9	negative selection	4692740
100213378	100213400	8	-	27760321	HL60	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.5741633859714883	[1468,1289]	[1683,1663]	6	hSpCas9	negative selection	4692741
100183411	100183433	8	+	27760321	HT1080	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.20555726733595833	[342,267]	[94,189]	1	hSpCas9	negative selection	4776200
100213124	100213146	8	-	27760321	HT1080	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	-0.5725324982504073	[727,567]	[227,113]	-3	hSpCas9	negative selection	4776201
100213232	100213254	8	+	27760321	HT1080	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-1.2470289724832422	[447,349]	[97,32]	-5	hSpCas9	negative selection	4776202
100213348	100213370	8	+	27760321	HT1080	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.684887953320975	[355,277]	[97,317]	3	hSpCas9	negative selection	4776203
100213378	100213400	8	-	27760321	HT1080	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	1.0255599255502617	[1289,1006]	[971,886]	5	hSpCas9	negative selection	4776204
100183411	100183433	8	+	27760321	HT29	viability after 7 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.6088932457813654	[342,267]	[646,582,573]	8	hSpCas9	negative selection	4859663
100213124	100213146	8	-	27760321	HT29	viability after 7 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.5164110158777442	[727,567]	[1232,1241,1106]	7	hSpCas9	negative selection	4859664
100213232	100213254	8	+	27760321	HT29	viability after 7 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	0.7103648400140985	[447,349]	[828,605,1072]	9	hSpCas9	negative selection	4859665
100213348	100213370	8	+	27760321	HT29	viability after 7 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.21214419572042914	[355,277]	[318,318,746]	3	hSpCas9	negative selection	4859666
100213378	100213400	8	-	27760321	HT29	viability after 7 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.41038795582098064	[1289,1006]	[1958,1794,2124]	6	hSpCas9	negative selection	4859667
100183411	100183433	8	+	27760321	HT29	viability after 25 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.5765006186383923	[342,267]	[429,632,501]	6	hSpCas9	negative selection	4943126
100213124	100213146	8	-	27760321	HT29	viability after 25 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.25002484935437286	[727,567]	[713,807,1157]	2	hSpCas9	negative selection	4943127
100213232	100213254	8	+	27760321	HT29	viability after 25 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.05183284999856075	[447,349]	[410,296,632]	0	hSpCas9	negative selection	4943128
100213348	100213370	8	+	27760321	HT29	viability after 25 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.308452517550356	[355,277]	[279,565,516]	3	hSpCas9	negative selection	4943129
100213378	100213400	8	-	27760321	HT29	viability after 25 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.4750109414106638	[1289,1006]	[1971,1991,1458]	5	hSpCas9	negative selection	4943130
100183411	100183433	8	+	27760321	HT29	viability after 22 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.40610769714134953	[342,267]	[546,482,370]	4	hSpCas9	negative selection	5026589
100213124	100213146	8	-	27760321	HT29	viability after 22 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.587979775778765	[727,567]	[1208,1013,1152]	6	hSpCas9	negative selection	5026590
100213232	100213254	8	+	27760321	HT29	viability after 22 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	0.22195252281146557	[447,349]	[540,631,439]	2	hSpCas9	negative selection	5026591
100213348	100213370	8	+	27760321	HT29	viability after 22 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.15562742340015862	[355,277]	[348,495,380]	1	hSpCas9	negative selection	5026592
100213378	100213400	8	-	27760321	HT29	viability after 22 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.4253619516713094	[1289,1006]	[1704,1981,1662]	4	hSpCas9	negative selection	5026593
100183411	100183433	8	+	27760321	HT29	viability after 19 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.988281556026966	[342,267]	[911,550,640]	8	hSpCas9	negative selection	5110052
100213124	100213146	8	-	27760321	HT29	viability after 19 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.35192985991282466	[727,567]	[955,911,968]	3	hSpCas9	negative selection	5110053
100213232	100213254	8	+	27760321	HT29	viability after 19 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.3561572708909801	[447,349]	[476,249,354]	-3	hSpCas9	negative selection	5110054
100213348	100213370	8	+	27760321	HT29	viability after 19 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	1.0404755863150663	[355,277]	[640,871,713]	9	hSpCas9	negative selection	5110055
100213378	100213400	8	-	27760321	HT29	viability after 19 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.7072006287879038	[1289,1006]	[2843,1776,1893]	7	hSpCas9	negative selection	5110056
100183411	100183433	8	+	27760321	HT29	viability after 16 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.7657436659355368	[342,267]	[675,442,636]	8	hSpCas9	negative selection	5193515
100213124	100213146	8	-	27760321	HT29	viability after 16 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.5356744934786726	[727,567]	[890,955,1292]	6	hSpCas9	negative selection	5193516
100213232	100213254	8	+	27760321	HT29	viability after 16 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	0.15525717299303218	[447,349]	[623,357,524]	1	hSpCas9	negative selection	5193517
100213348	100213370	8	+	27760321	HT29	viability after 16 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.4106450075086763	[355,277]	[444,463,502]	4	hSpCas9	negative selection	5193518
100213378	100213400	8	-	27760321	HT29	viability after 16 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.5025808291987386	[1289,1006]	[1981,2025,1462]	6	hSpCas9	negative selection	5193519
100183411	100183433	8	+	27760321	HT29	viability after 13 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.42611066626755834	[342,267]	[321,767,346]	5	hSpCas9	negative selection	5276978
100213124	100213146	8	-	27760321	HT29	viability after 13 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.2382107723187119	[727,567]	[996,798,864]	3	hSpCas9	negative selection	5276979
100213232	100213254	8	+	27760321	HT29	viability after 13 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.27389371124823303	[447,349]	[494,299,354]	-3	hSpCas9	negative selection	5276980
100213348	100213370	8	+	27760321	HT29	viability after 13 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.860852239060384	[355,277]	[697,696,611]	9	hSpCas9	negative selection	5276981
100213378	100213400	8	-	27760321	HT29	viability after 13 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.39991361293132277	[1289,1006]	[1858,1682,1732]	5	hSpCas9	negative selection	5276982
100183411	100183433	8	+	27760321	HT29	viability after 10 days	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	0.8390420363623365	[342,267]	[670,706,669]	9	hSpCas9	negative selection	5360441
100213124	100213146	8	-	27760321	HT29	viability after 10 days	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	0.10831728401647656	[727,567]	[769,952,894]	1	hSpCas9	negative selection	5360442
100213232	100213254	8	+	27760321	HT29	viability after 10 days	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	0.19939248650596977	[447,349]	[615,310,776]	2	hSpCas9	negative selection	5360443
100213348	100213370	8	+	27760321	HT29	viability after 10 days	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	0.29049702692745843	[355,277]	[449,374,620]	4	hSpCas9	negative selection	5360444
100213378	100213400	8	-	27760321	HT29	viability after 10 days	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.6046159058074814	[1289,1006]	[2167,2364,2016]	8	hSpCas9	negative selection	5360445
100183411	100183433	8	+	27760321	MOLM13	viability	CCAAGGGATTACGCGGAATGGG	SPAG1	ENSG00000104450	-1.928841283369403	[468,342]	[17,94]	-8	hSpCas9	negative selection	5443904
100213124	100213146	8	-	27760321	MOLM13	viability	GTTGCCCATGACGCACGGCTGG	SPAG1	ENSG00000104450	-0.45241781051673946	[950,727]	[450,251]	-3	hSpCas9	negative selection	5443905
100213232	100213254	8	+	27760321	MOLM13	viability	CGCGGACAAGCGGAGCCCACGG	SPAG1	ENSG00000104450	-0.58448609480194	[577,447]	[108,261]	-4	hSpCas9	negative selection	5443906
100213348	100213370	8	+	27760321	MOLM13	viability	GCAACGAGCTGTTCCGAAGCGG	SPAG1	ENSG00000104450	-0.7047562011764135	[466,355]	[156,127]	-4	hSpCas9	negative selection	5443907
100213378	100213400	8	-	27760321	MOLM13	viability	GAGTACTTGCCGGCCGCCTCGG	SPAG1	ENSG00000104450	0.36762042466002787	[1468,1289]	[1544,539]	2	hSpCas9	negative selection	5443908
100158233	100158256	8	+	27661255	K562	viability	GCGAGGGAGAAGGAGCGATGGGG	SPAG1	ENSG00000104450	0.14279212684356457	[1836,2055]	[1832,1610]	5	dCas9-KRAB	negative selection	5513671
100158233	100158256	8	+	27661255	K562	viability	GCGAGGGAGAAGGAGCGATGGGG	SPAG1	ENSG00000104450	0.14279212684356457	[1836,2055]	[1832,1610]	5	dCas9-KRAB	negative selection	5513672
100158338	100158361	8	+	27661255	K562	viability	GCTGGAGTTCCCGGGTCCACCGG	SPAG1	ENSG00000104450	0.02647185364047372	[671,590]	[555,479]	0	dCas9-KRAB	negative selection	5513673
100158396	100158419	8	+	27661255	K562	viability	GGAGCAATCTGAGACCATTGCGG	SPAG1	ENSG00000104450	0.1419194157049558	[1167,1067]	[1115,876]	5	dCas9-KRAB	negative selection	5513674
100158046	100158069	8	-	27661255	K562	viability	GGCTGCACTGCAGGTGCTGAGGG	SPAG1	ENSG00000104450	0.3077856626903889	[268,446]	[366,336]	8	dCas9-KRAB	negative selection	5513675
100158073	100158096	8	-	27661255	K562	viability	GGAAGTCGCGGCCGCCGCACCGG	SPAG1	ENSG00000104450	0.20208795305285698	[910,1078]	[917,905]	7	dCas9-KRAB	negative selection	5513676
100158410	100158433	8	-	27661255	K562	viability	GCCCCACAGCCCAGCCGCAATGG	SPAG1	ENSG00000104450	0.22529054525373615	[609,848]	[721,638]	7	dCas9-KRAB	negative selection	5513677
100162399	100162422	8	+	27260156	BXPC3	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.044263648882952866	[143]	[85,131,139,286]	1	hSpCas9	negative selection	5572431
100177903	100177926	8	-	27260156	BXPC3	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.25823474359960263	[257]	[270,322,335,369]	5	hSpCas9	negative selection	5572432
100162288	100162311	8	+	27260156	BXPC3	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.05829005532790221	[632]	[668,532,544,813]	-1	hSpCas9	negative selection	5572433
100177891	100177914	8	+	27260156	BXPC3	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.19525490114151572	[588]	[593,740,768,724]	4	hSpCas9	negative selection	5636683
100165836	100165859	8	-	27260156	BXPC3	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.28686108942218813	[676]	[695,763,994,1041]	6	hSpCas9	negative selection	5636684
100165879	100165902	8	+	27260156	BXPC3	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.1287729004536406	[501]	[653,569,319,756]	3	hSpCas9	negative selection	5636685
100162399	100162422	8	+	27260156	A673	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.11860345749348467	[143]	[205,238,175,126]	3	hSpCas9	negative selection	5693682
100177903	100177926	8	-	27260156	A673	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.2847182608482164	[257]	[359,296,370,486]	6	hSpCas9	negative selection	5693683
100162288	100162311	8	+	27260156	A673	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.04371465222271609	[632]	[900,785,656,803]	1	hSpCas9	negative selection	5693684
100177891	100177914	8	+	27260156	A673	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.06100272810088303	[588]	[871,638,687,770]	1	hSpCas9	negative selection	5757934
100165836	100165859	8	-	27260156	A673	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.042941422112145244	[676]	[803,737,743,876]	-1	hSpCas9	negative selection	5757935
100165879	100165902	8	+	27260156	A673	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.11499714223963023	[501]	[682,571,559,809]	2	hSpCas9	negative selection	5757936
100162399	100162422	8	+	27260156	A375	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.6643642507135747	[143]	[190,381,206,296]	8	hSpCas9	negative selection	5814933
100177903	100177926	8	-	27260156	A375	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.2908188402192776	[257]	[334,468,324,358]	4	hSpCas9	negative selection	5814934
100162288	100162311	8	+	27260156	A375	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.08803515570913406	[632]	[839,875,694,746]	1	hSpCas9	negative selection	5814935
100177891	100177914	8	+	27260156	A375	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.3829873565904552	[588]	[799,849,1125,811]	5	hSpCas9	negative selection	5879185
100165836	100165859	8	-	27260156	A375	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.2750386781656924	[676]	[1171,1398,704,647]	4	hSpCas9	negative selection	5879186
100165879	100165902	8	+	27260156	A375	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.22669497565955737	[501]	[553,997,618,620]	3	hSpCas9	negative selection	5879187
100162399	100162422	8	+	27260156	COLO741	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.34565283323919016	[143]	[318,197,193]	7	hSpCas9	negative selection	5936184
100177903	100177926	8	-	27260156	COLO741	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.07898770846126046	[257]	[426,367,261]	1	hSpCas9	negative selection	5936185
100162288	100162311	8	+	27260156	COLO741	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.07854256944465704	[632]	[1027,753,796]	1	hSpCas9	negative selection	5936186
100177891	100177914	8	+	27260156	COLO741	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.13243489806285072	[588]	[757,576,725]	-3	hSpCas9	negative selection	6000436
100165836	100165859	8	-	27260156	COLO741	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.08612614804572388	[676]	[813,928,707]	-2	hSpCas9	negative selection	6000437
100165879	100165902	8	+	27260156	COLO741	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.05674551654028101	[501]	[743,646,618]	1	hSpCas9	negative selection	6000438
100162399	100162422	8	+	27260156	CAL120	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.15175500740240078	[143]	[190,174,122]	-2	hSpCas9	negative selection	6057435
100177903	100177926	8	-	27260156	CAL120	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.026138119650616964	[257]	[296,219,423]	0	hSpCas9	negative selection	6057436
100162288	100162311	8	+	27260156	CAL120	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.2753578945674292	[632]	[641,663,644]	-4	hSpCas9	negative selection	6057437
100177891	100177914	8	+	27260156	CAL120	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.29719038202152487	[588]	[1224,554,968]	4	hSpCas9	negative selection	6121687
100165836	100165859	8	-	27260156	CAL120	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.11644691400525486	[676]	[1058,1048,657]	1	hSpCas9	negative selection	6121688
100165879	100165902	8	+	27260156	CAL120	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.1278177108815726	[501]	[657,720,664]	2	hSpCas9	negative selection	6121689
100162399	100162422	8	+	27260156	CADOES1	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.04543908957649356	[143]	[138,138,81,194]	0	hSpCas9	negative selection	6178686
100177903	100177926	8	-	27260156	CADOES1	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.2549162131012561	[257]	[178,284,154,184]	-4	hSpCas9	negative selection	6178687
100162288	100162311	8	+	27260156	CADOES1	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.2175449802649121	[632]	[537,410,543,532]	-4	hSpCas9	negative selection	6178688
100177891	100177914	8	+	27260156	CADOES1	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.18386738658745477	[588]	[629,485,717,655]	3	hSpCas9	negative selection	6242938
100165836	100165859	8	-	27260156	CADOES1	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.1761281173379341	[676]	[766,695,631,751]	3	hSpCas9	negative selection	6242939
100165879	100165902	8	+	27260156	CADOES1	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.30135953064138143	[501]	[486,643,461,713]	5	hSpCas9	negative selection	6242940
100162399	100162422	8	+	27260156	EWS502	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.45965200898716363	[143]	[297,172,148,207]	7	hSpCas9	negative selection	6299937
100177903	100177926	8	-	27260156	EWS502	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3155969625713303	[257]	[332,293,355,381]	5	hSpCas9	negative selection	6299938
100162288	100162311	8	+	27260156	EWS502	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.1723406534200576	[632]	[676,748,788,818]	3	hSpCas9	negative selection	6299939
100177891	100177914	8	+	27260156	EWS502	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.06324637215077761	[588]	[598,710,543,520]	-1	hSpCas9	negative selection	6364189
100165836	100165859	8	-	27260156	EWS502	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.6509489569764182	[676]	[938,1722,636,1196]	9	hSpCas9	negative selection	6364190
100165879	100165902	8	+	27260156	EWS502	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.20824843304335083	[501]	[564,527,665,712]	3	hSpCas9	negative selection	6364191
100162399	100162422	8	+	27260156	EW8	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.11939136345049306	[143]	[207,104,88,119]	2	hSpCas9	negative selection	6421188
100177903	100177926	8	-	27260156	EW8	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.44050708468812666	[257]	[251,278,310,283]	7	hSpCas9	negative selection	6421189
100162288	100162311	8	+	27260156	EW8	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.0033314103392411143	[632]	[518,390,524,617]	0	hSpCas9	negative selection	6421190
100177891	100177914	8	+	27260156	EW8	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.05316057835721816	[588]	[521,577,254,525]	-1	hSpCas9	negative selection	6485440
100165836	100165859	8	-	27260156	EW8	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.10922176633309244	[676]	[577,671,420,721]	2	hSpCas9	negative selection	6485441
100165879	100165902	8	+	27260156	EW8	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.12507195684556344	[501]	[439,453,389,497]	2	hSpCas9	negative selection	6485442
100162399	100162422	8	+	27260156	CORL105	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.49994684150598667	[143]	[340,195,168,240]	8	hSpCas9	negative selection	6542439
100177903	100177926	8	-	27260156	CORL105	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3874252979992773	[257]	[443,348,433,361]	7	hSpCas9	negative selection	6542440
100162288	100162311	8	+	27260156	CORL105	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.14506328638848082	[632]	[662,522,1381,797]	2	hSpCas9	negative selection	6542441
100177891	100177914	8	+	27260156	CORL105	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.1411076418735778	[588]	[577,596,696,638]	-3	hSpCas9	negative selection	6606691
100165836	100165859	8	-	27260156	CORL105	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.22929524472436935	[676]	[1077,885,832,909]	4	hSpCas9	negative selection	6606692
100165879	100165902	8	+	27260156	CORL105	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.4688750122034354	[501]	[839,742,973,723]	8	hSpCas9	negative selection	6606693
100162399	100162422	8	+	27260156	HS294T	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.21979909493429228	[143]	[60,41,78,80]	3	hSpCas9	negative selection	6663690
100177903	100177926	8	-	27260156	HS294T	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.15497048972604022	[257]	[71,82,145,154]	2	hSpCas9	negative selection	6663691
100162288	100162311	8	+	27260156	HS294T	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.32919758099254787	[632]	[178,336,348,388]	4	hSpCas9	negative selection	6663692
100177891	100177914	8	+	27260156	HS294T	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.05776656757784615	[588]	[219,219,251,174]	-1	hSpCas9	negative selection	6727942
100165836	100165859	8	-	27260156	HS294T	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.3229513385223236	[676]	[258,406,221,443]	4	hSpCas9	negative selection	6727943
100165879	100165902	8	+	27260156	HS294T	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.17420807880193517	[501]	[148,183,200,151]	-2	hSpCas9	negative selection	6727944
100162399	100162422	8	+	27260156	HCC44	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.7935224182324334	[143]	[272,205,573,138]	9	hSpCas9	negative selection	6784941
100177903	100177926	8	-	27260156	HCC44	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.7651168576724499	[257]	[244,531,728,485]	9	hSpCas9	negative selection	6784942
100162288	100162311	8	+	27260156	HCC44	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.28280857032482426	[632]	[951,680,1092,824]	4	hSpCas9	negative selection	6784943
100177891	100177914	8	+	27260156	HCC44	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.13741556962663753	[588]	[874,633,603,813]	2	hSpCas9	negative selection	6849193
100165836	100165859	8	-	27260156	HCC44	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.20600200550344472	[676]	[821,1073,1092,550]	3	hSpCas9	negative selection	6849194
100165879	100165902	8	+	27260156	HCC44	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.2235801454146048	[501]	[790,418,804,700]	3	hSpCas9	negative selection	6849195
100162399	100162422	8	+	27260156	G402	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.10879555634837268	[143]	[234,179,154,192]	1	hSpCas9	negative selection	6906192
100177903	100177926	8	-	27260156	G402	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.05849826156462701	[257]	[284,378,338,304]	0	hSpCas9	negative selection	6906193
100162288	100162311	8	+	27260156	G402	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.0665267965478128	[632]	[730,1121,488,892]	0	hSpCas9	negative selection	6906194
100177891	100177914	8	+	27260156	G402	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.3010197049278958	[588]	[889,953,834,864]	4	hSpCas9	negative selection	6970444
100165836	100165859	8	-	27260156	G402	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.39174608406786515	[676]	[2042,850,690,880]	5	hSpCas9	negative selection	6970445
100165879	100165902	8	+	27260156	G402	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.24390748508712717	[501]	[1234,541,700,494]	3	hSpCas9	negative selection	6970446
100162399	100162422	8	+	27260156	L33	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.2219713944848416	[143]	[214,68,91,140]	5	hSpCas9	negative selection	7027443
100177903	100177926	8	-	27260156	L33	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.33378714143114296	[257]	[240,153,173,407]	7	hSpCas9	negative selection	7027444
100162288	100162311	8	+	27260156	L33	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.34815660020887634	[632]	[778,347,460,860]	7	hSpCas9	negative selection	7027445
100177891	100177914	8	+	27260156	L33	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.23880000119662964	[588]	[518,208,272,531]	-5	hSpCas9	negative selection	7091695
100165836	100165859	8	-	27260156	L33	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.04147844311770743	[676]	[724,352,308,706]	0	hSpCas9	negative selection	7091696
100165879	100165902	8	+	27260156	L33	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.18937665513672153	[501]	[499,282,328,576]	4	hSpCas9	negative selection	7091697
100162399	100162422	8	+	27260156	K562	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.8144835368846362	[143]	[293,221,138,245]	9	hSpCas9	negative selection	7148694
100177903	100177926	8	-	27260156	K562	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.6050615526824334	[257]	[124,170,111,187]	-6	hSpCas9	negative selection	7148695
100162288	100162311	8	+	27260156	K562	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.28809131813001515	[632]	[331,976,769,651]	3	hSpCas9	negative selection	7148696
100177891	100177914	8	+	27260156	K562	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.12147672491931832	[588]	[665,693,361,564]	1	hSpCas9	negative selection	7212946
100165836	100165859	8	-	27260156	K562	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.17920126290684946	[676]	[679,865,691,509]	2	hSpCas9	negative selection	7212947
100165879	100165902	8	+	27260156	K562	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.03542336693010039	[501]	[437,314,336,618]	0	hSpCas9	negative selection	7212948
100162399	100162422	8	+	27260156	HT29	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.2879101663009225	[143]	[156,262,235,198]	5	hSpCas9	negative selection	7269945
100177903	100177926	8	-	27260156	HT29	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.13230948594375302	[257]	[372,235,250,284]	-3	hSpCas9	negative selection	7269946
100162288	100162311	8	+	27260156	HT29	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.2708004442471336	[632]	[967,1084,883,801]	5	hSpCas9	negative selection	7269947
100177891	100177914	8	+	27260156	HT29	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.272141398031619	[588]	[805,944,976,741]	5	hSpCas9	negative selection	7334197
100165836	100165859	8	-	27260156	HT29	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.150272549618103	[676]	[974,1039,1081,603]	3	hSpCas9	negative selection	7334198
100165879	100165902	8	+	27260156	HT29	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.11478715228556319	[501]	[561,678,473,546]	-2	hSpCas9	negative selection	7334199
100162399	100162422	8	+	27260156	MHHES1	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.5885111332692965	[143]	[288,339,135,223]	8	hSpCas9	negative selection	7391196
100177903	100177926	8	-	27260156	MHHES1	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.4629239024209857	[257]	[207,322,153,172]	-7	hSpCas9	negative selection	7391197
100162288	100162311	8	+	27260156	MHHES1	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.09273451372784774	[632]	[629,1107,849,513]	1	hSpCas9	negative selection	7391198
100177891	100177914	8	+	27260156	MHHES1	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.16241167753002592	[588]	[638,623,679,455]	-3	hSpCas9	negative selection	7455448
100165836	100165859	8	-	27260156	MHHES1	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.5105281525254717	[676]	[1577,1074,909,865]	8	hSpCas9	negative selection	7455449
100165879	100165902	8	+	27260156	MHHES1	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.20505207488553445	[501]	[914,699,675,397]	4	hSpCas9	negative selection	7455450
100162399	100162422	8	+	27260156	MEWO	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.13315541032464773	[143]	[124,106,206]	3	hSpCas9	negative selection	7512447
100177903	100177926	8	-	27260156	MEWO	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3910081597224813	[257]	[282,313,345]	7	hSpCas9	negative selection	7512448
100162288	100162311	8	+	27260156	MEWO	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.17544495834665857	[632]	[735,513,769]	4	hSpCas9	negative selection	7512449
100177891	100177914	8	+	27260156	MEWO	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.1831627246222649	[588]	[736,419,347]	-4	hSpCas9	negative selection	7576699
100165836	100165859	8	-	27260156	MEWO	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.23238563418976332	[676]	[659,483,496]	-5	hSpCas9	negative selection	7576700
100165879	100165902	8	+	27260156	MEWO	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.28525484478740576	[501]	[705,522,513]	6	hSpCas9	negative selection	7576701
100162399	100162422	8	+	27260156	LNCAPCLONEFGC	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.41537301162010043	[143]	[154,202,138,191]	6	hSpCas9	negative selection	7633698
100177903	100177926	8	-	27260156	LNCAPCLONEFGC	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.16521020042614087	[257]	[210,120,219,288]	-3	hSpCas9	negative selection	7633699
100162288	100162311	8	+	27260156	LNCAPCLONEFGC	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.042562464301777014	[632]	[640,503,747,496]	0	hSpCas9	negative selection	7633700
100177891	100177914	8	+	27260156	LNCAPCLONEFGC	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.14698102640883004	[588]	[681,544,725,430]	2	hSpCas9	negative selection	7697950
100165836	100165859	8	-	27260156	LNCAPCLONEFGC	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.30237762262639156	[676]	[705,756,860,716]	5	hSpCas9	negative selection	7697951
100165879	100165902	8	+	27260156	LNCAPCLONEFGC	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.3481747056186631	[501]	[497,481,699,660]	5	hSpCas9	negative selection	7697952
100162399	100162422	8	+	27260156	PANC0327	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.261842297376232	[143]	[80,86,58,199]	-4	hSpCas9	negative selection	7754949
100177903	100177926	8	-	27260156	PANC0327	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.5629095080173763	[257]	[224,165,801,174]	7	hSpCas9	negative selection	7754950
100162288	100162311	8	+	27260156	PANC0327	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.3409416752761542	[632]	[446,615,704,1070]	5	hSpCas9	negative selection	7754951
100177891	100177914	8	+	27260156	PANC0327	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.5053628025794236	[588]	[316,369,441,340]	-6	hSpCas9	negative selection	7819201
100165836	100165859	8	-	27260156	PANC0327	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.35133277374559824	[676]	[918,481,872,843]	5	hSpCas9	negative selection	7819202
100165879	100165902	8	+	27260156	PANC0327	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.08156305548491488	[501]	[366,473,291,540]	-1	hSpCas9	negative selection	7819203
100162399	100162422	8	+	27260156	NCIH2009	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.4825533581067811	[143]	[374,192,294]	6	hSpCas9	negative selection	7876200
100177903	100177926	8	-	27260156	NCIH2009	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.20820783903856033	[257]	[506,387,371]	3	hSpCas9	negative selection	7876201
100162288	100162311	8	+	27260156	NCIH2009	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.3204933277498419	[632]	[1152,1210,954]	4	hSpCas9	negative selection	7876202
100177891	100177914	8	+	27260156	NCIH2009	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.2845374560250354	[588]	[1017,1024,964]	4	hSpCas9	negative selection	7940452
100165836	100165859	8	-	27260156	NCIH2009	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.16790910705239503	[676]	[1233,838,1150]	2	hSpCas9	negative selection	7940453
100165879	100165902	8	+	27260156	NCIH2009	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.03129273835168209	[501]	[975,566,659]	0	hSpCas9	negative selection	7940454
100162399	100162422	8	+	27260156	NCIH1373	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.8022840912481343	[143]	[503,158,382,29]	7	hSpCas9	negative selection	7997451
100177903	100177926	8	-	27260156	NCIH1373	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.4051594462618604	[257]	[339,153,222,46]	-4	hSpCas9	negative selection	7997452
100162288	100162311	8	+	27260156	NCIH1373	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.2073700143953216	[632]	[443,1084,640,270]	2	hSpCas9	negative selection	7997453
100177891	100177914	8	+	27260156	NCIH1373	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.24105972791652913	[588]	[828,561,783,258]	2	hSpCas9	negative selection	8061703
100165836	100165859	8	-	27260156	NCIH1373	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.317913099103839	[676]	[1022,568,1025,322]	3	hSpCas9	negative selection	8061704
100165879	100165902	8	+	27260156	NCIH1373	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.05653977694992218	[501]	[486,648,704,121]	0	hSpCas9	negative selection	8061705
100162399	100162422	8	+	27260156	PATU8902	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	1.0237373132225218	[143]	[433,561,272,391]	9	hSpCas9	negative selection	8118702
100177903	100177926	8	-	27260156	PATU8902	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.003886648451069008	[257]	[137,588,239,525]	0	hSpCas9	negative selection	8118703
100162288	100162311	8	+	27260156	PATU8902	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.3691722309854882	[632]	[863,393,820,595]	-4	hSpCas9	negative selection	8118704
100177891	100177914	8	+	27260156	PATU8902	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.0747296034157679	[588]	[1206,638,447,910]	0	hSpCas9	negative selection	8182954
100165836	100165859	8	-	27260156	PATU8902	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.8289620333235956	[676]	[1521,1625,1045,2790]	8	hSpCas9	negative selection	8182955
100165879	100165902	8	+	27260156	PATU8902	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.2730557079622849	[501]	[936,1223,585,671]	2	hSpCas9	negative selection	8182956
100162399	100162422	8	+	27260156	PANC1	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.3923637611310291	[143]	[136,217,91,203]	6	hSpCas9	negative selection	8239953
100177903	100177926	8	-	27260156	PANC1	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.10610307134960806	[257]	[226,370,181,202]	1	hSpCas9	negative selection	8239954
100162288	100162311	8	+	27260156	PANC1	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.12558865244645162	[632]	[483,558,505,455]	-2	hSpCas9	negative selection	8239955
100177891	100177914	8	+	27260156	PANC1	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.24462919841597935	[588]	[559,485,733,587]	4	hSpCas9	negative selection	8304205
100165836	100165859	8	-	27260156	PANC1	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.3379498708617561	[676]	[966,730,661,585]	5	hSpCas9	negative selection	8304206
100165879	100165902	8	+	27260156	PANC1	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.09451281606170708	[501]	[523,485,334,486]	1	hSpCas9	negative selection	8304207
100162399	100162422	8	+	27260156	PANC0813	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.33969189324311255	[143]	[274,125,182,273]	5	hSpCas9	negative selection	8361204
100177903	100177926	8	-	27260156	PANC0813	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.2517153446383589	[257]	[379,442,243,375]	4	hSpCas9	negative selection	8361205
100162288	100162311	8	+	27260156	PANC0813	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.09546474646712755	[632]	[649,771,657,697]	-1	hSpCas9	negative selection	8361206
100177891	100177914	8	+	27260156	PANC0813	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.07001237427423834	[588]	[752,660,492,731]	-1	hSpCas9	negative selection	8425456
100165836	100165859	8	-	27260156	PANC0813	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.09445685907741319	[676]	[769,758,1056,804]	1	hSpCas9	negative selection	8425457
100165879	100165902	8	+	27260156	PANC0813	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.4271430351786517	[501]	[626,938,972,625]	7	hSpCas9	negative selection	8425458
100162399	100162422	8	+	27260156	RDES	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.6341181380510179	[143]	[208,233,268,332]	8	hSpCas9	negative selection	8482455
100177903	100177926	8	-	27260156	RDES	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3077010559857014	[257]	[337,279,372,501]	5	hSpCas9	negative selection	8482456
100162288	100162311	8	+	27260156	RDES	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.08312462555357136	[632]	[602,639,662,870]	-1	hSpCas9	negative selection	8482457
100177891	100177914	8	+	27260156	RDES	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.2937743185179503	[588]	[757,768,991,797]	5	hSpCas9	negative selection	8546707
100165836	100165859	8	-	27260156	RDES	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.38161755305618716	[676]	[731,935,1085,1405]	6	hSpCas9	negative selection	8546708
100165879	100165902	8	+	27260156	RDES	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.014766665161462258	[501]	[433,533,611,808]	0	hSpCas9	negative selection	8546709
100162399	100162422	8	+	27260156	PC3	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.08657782751120036	[143]	[79,202,237,136]	2	hSpCas9	negative selection	8603706
100177903	100177926	8	-	27260156	PC3	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.3641398300328831	[257]	[314,179,607,296]	7	hSpCas9	negative selection	8603707
100162288	100162311	8	+	27260156	PC3	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.09974834513307196	[632]	[360,537,879,742]	-2	hSpCas9	negative selection	8603708
100177891	100177914	8	+	27260156	PC3	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	-0.20582802874999945	[588]	[301,402,1076,526]	-5	hSpCas9	negative selection	8667958
100165836	100165859	8	-	27260156	PC3	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.04026546153719368	[676]	[419,939,858,656]	0	hSpCas9	negative selection	8667959
100165879	100165902	8	+	27260156	PC3	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.18394872772196894	[501]	[289,366,779,484]	-4	hSpCas9	negative selection	8667960
100162399	100162422	8	+	27260156	PATU8988T	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	-0.5393996026892103	[143]	[82,124,119,53]	-7	hSpCas9	negative selection	8724957
100177903	100177926	8	-	27260156	PATU8988T	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.10981721862233218	[257]	[188,381,122,246]	-2	hSpCas9	negative selection	8724958
100162288	100162311	8	+	27260156	PATU8988T	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.067146054227542	[632]	[631,561,610,522]	-1	hSpCas9	negative selection	8724959
100177891	100177914	8	+	27260156	PATU8988T	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.11572397777151971	[588]	[500,530,669,767]	2	hSpCas9	negative selection	8789209
100165836	100165859	8	-	27260156	PATU8988T	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.08157071096616131	[676]	[455,907,765,654]	1	hSpCas9	negative selection	8789210
100165879	100165902	8	+	27260156	PATU8988T	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.08924823014895318	[501]	[438,412,540,422]	-1	hSpCas9	negative selection	8789211
100162399	100162422	8	+	27260156	T47D	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.29066996496114306	[143]	[196,179,226,218]	6	hSpCas9	negative selection	8846208
100177903	100177926	8	-	27260156	T47D	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.21945428767180042	[257]	[244,228,267,293]	-5	hSpCas9	negative selection	8846209
100162288	100162311	8	+	27260156	T47D	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.17264827148072653	[632]	[783,807,904,827]	3	hSpCas9	negative selection	8846210
100177891	100177914	8	+	27260156	T47D	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.11486328901295723	[588]	[745,649,788,795]	2	hSpCas9	negative selection	8910460
100165836	100165859	8	-	27260156	T47D	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.2517583244630246	[676]	[785,950,1016,1000]	5	hSpCas9	negative selection	8910461
100165879	100165902	8	+	27260156	T47D	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.16164671706194533	[501]	[748,650,594,621]	3	hSpCas9	negative selection	8910462
100162399	100162422	8	+	27260156	SU8686	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.5910457150816553	[143]	[180,206,235,244]	8	hSpCas9	negative selection	8967459
100177903	100177926	8	-	27260156	SU8686	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.37969124937038773	[257]	[347,263,352,378]	6	hSpCas9	negative selection	8967460
100162288	100162311	8	+	27260156	SU8686	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.10466032273917475	[632]	[603,600,645,895]	1	hSpCas9	negative selection	8967461
100177891	100177914	8	+	27260156	SU8686	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.07381003957580085	[588]	[534,529,507,952]	1	hSpCas9	negative selection	9031711
100165836	100165859	8	-	27260156	SU8686	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.014038211432944037	[676]	[563,595,846,736]	0	hSpCas9	negative selection	9031712
100165879	100165902	8	+	27260156	SU8686	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.17065562290074054	[501]	[471,493,566,753]	3	hSpCas9	negative selection	9031713
100162399	100162422	8	+	27260156	SKES1	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.2012226525253742	[143]	[170,191,121,176]	4	hSpCas9	negative selection	9088710
100177903	100177926	8	-	27260156	SKES1	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.038074416831800684	[257]	[301,294,244,225]	0	hSpCas9	negative selection	9088711
100162288	100162311	8	+	27260156	SKES1	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.1334619793375213	[632]	[616,727,538,451]	-3	hSpCas9	negative selection	9088712
100177891	100177914	8	+	27260156	SKES1	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.06778503765744393	[588]	[817,642,655,396]	1	hSpCas9	negative selection	9152962
100165836	100165859	8	-	27260156	SKES1	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.03310104738482611	[676]	[832,738,745,487]	0	hSpCas9	negative selection	9152963
100165879	100165902	8	+	27260156	SKES1	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.025991271920329073	[501]	[480,608,558,404]	0	hSpCas9	negative selection	9152964
100162399	100162422	8	+	27260156	TOV112D	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.24155836151112142	[143]	[175,191,202,117]	4	hSpCas9	negative selection	9209961
100177903	100177926	8	-	27260156	TOV112D	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	0.5778132548503987	[257]	[415,272,408,417]	9	hSpCas9	negative selection	9209962
100162288	100162311	8	+	27260156	TOV112D	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.11223115465045713	[632]	[806,833,467,677]	2	hSpCas9	negative selection	9209963
100177891	100177914	8	+	27260156	TOV112D	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.09481532470151771	[588]	[641,1000,547,425]	1	hSpCas9	negative selection	9274213
100165836	100165859	8	-	27260156	TOV112D	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.087089242306636	[676]	[663,654,545,691]	-2	hSpCas9	negative selection	9274214
100165879	100165902	8	+	27260156	TOV112D	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.0011779623220769808	[501]	[518,641,331,552]	0	hSpCas9	negative selection	9274215
100162399	100162422	8	+	27260156	TC71	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.22776032605654195	[143]	[178,190,135,220]	5	hSpCas9	negative selection	9331212
100177903	100177926	8	-	27260156	TC71	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.08049922934888476	[257]	[322,243,249,225]	-2	hSpCas9	negative selection	9331213
100162288	100162311	8	+	27260156	TC71	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	0.24614676038142463	[632]	[778,704,852,885]	6	hSpCas9	negative selection	9331214
100177891	100177914	8	+	27260156	TC71	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.010439203249852758	[588]	[610,663,567,707]	0	hSpCas9	negative selection	9395464
100165836	100165859	8	-	27260156	TC71	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	-0.09612087587676643	[676]	[734,726,633,615]	-2	hSpCas9	negative selection	9395465
100165879	100165902	8	+	27260156	TC71	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	0.12289125285953556	[501]	[372,666,600,714]	3	hSpCas9	negative selection	9395466
100162399	100162422	8	+	27260156	TC32	viability	AAAAAATTCTTTGCGTGCTCAGG	SPAG1	ENSG00000104450	0.4594701156428074	[143]	[200,117,208,235]	7	hSpCas9	negative selection	9452463
100177903	100177926	8	-	27260156	TC32	viability	CAGCTGTTTGAACCACGAACTGG	SPAG1	ENSG00000104450	-0.19836514295125451	[257]	[215,164,284,194]	-4	hSpCas9	negative selection	9452464
100162288	100162311	8	+	27260156	TC32	viability	CCAAAGATTATCCATCATTGTGG	SPAG1	ENSG00000104450	-0.021276452915976907	[632]	[451,485,627,818]	0	hSpCas9	negative selection	9452465
100177891	100177914	8	+	27260156	TC32	viability	GATAATTTGCCTCCAGTTCGTGG	SPAG1	ENSG00000104450	0.03112910149491399	[588]	[558,388,880,519]	0	hSpCas9	negative selection	9516715
100165836	100165859	8	-	27260156	TC32	viability	TCACAAAATTCTGTAAGTTCAGG	SPAG1	ENSG00000104450	0.1796192932327989	[676]	[690,415,1234,707]	3	hSpCas9	negative selection	9516716
100165879	100165902	8	+	27260156	TC32	viability	CCCCTGAAAGCAGAGCTTTGAGG	SPAG1	ENSG00000104450	-0.27608053856450626	[501]	[350,284,519,449]	-5	hSpCas9	negative selection	9516717
100177920	100177943	8	+	27869803	HPAFII	viability after 27 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.7476816938121772	[456]	[171,118,NaN]	-5	hSpCas9	negative selection	9600828
100183409	100183432	8	+	27869803	HPAFII	viability after 27 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	1.134654680120602	[588]	[956,448,NaN]	8	hSpCas9	negative selection	9600829
100187217	100187240	8	+	27869803	HPAFII	viability after 27 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.46178493022354467	[141]	[116,90,NaN]	4	hSpCas9	negative selection	9600830
100191475	100191498	8	+	27869803	HPAFII	viability after 27 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.570098633618557	[171]	[103,24,NaN]	-4	hSpCas9	negative selection	9600831
100213348	100213371	8	+	27869803	HPAFII	viability after 27 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.7286763859616252	[311]	[159,364,NaN]	6	hSpCas9	negative selection	9600832
100220302	100220325	8	+	27869803	HPAFII	viability after 27 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.7547574573900779	[233]	[67,76,NaN]	-5	hSpCas9	negative selection	9600833
100177920	100177943	8	+	27869803	HPAFII	viability after 15 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.27169088009129216	[456]	[523,333,NaN]	-3	hSpCas9	negative selection	9683277
100183409	100183432	8	+	27869803	HPAFII	viability after 15 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.6549501446140111	[588]	[1407,740,NaN]	7	hSpCas9	negative selection	9683278
100187217	100187240	8	+	27869803	HPAFII	viability after 15 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.5823847260201702	[141]	[320,170,NaN]	6	hSpCas9	negative selection	9683279
100191475	100191498	8	+	27869803	HPAFII	viability after 15 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	0.05854180161128486	[171]	[284,134,NaN]	0	hSpCas9	negative selection	9683280
100213348	100213371	8	+	27869803	HPAFII	viability after 15 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.7630830361698924	[311]	[417,662,NaN]	8	hSpCas9	negative selection	9683281
100220302	100220325	8	+	27869803	HPAFII	viability after 15 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.2507278841892414	[233]	[342,271,NaN]	3	hSpCas9	negative selection	9683282
100177920	100177943	8	+	27869803	ASPC1	viability after 36 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.2166295400457392	[1103]	[430,335,254]	-2	hSpCas9	negative selection	9765726
100183409	100183432	8	+	27869803	ASPC1	viability after 36 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.11156766267587614	[1307]	[716,490,332]	1	hSpCas9	negative selection	9765727
100187217	100187240	8	+	27869803	ASPC1	viability after 36 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.1520421507233642	[340]	[241,268,273]	9	hSpCas9	negative selection	9765728
100191475	100191498	8	+	27869803	ASPC1	viability after 36 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	0.47288117999680856	[319]	[132,43,256]	5	hSpCas9	negative selection	9765729
100213348	100213371	8	+	27869803	ASPC1	viability after 36 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.25441731926634337	[1256]	[276,597,244]	-3	hSpCas9	negative selection	9765730
100220302	100220325	8	+	27869803	ASPC1	viability after 36 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.23413003357868428	[580]	[184,311,50]	-2	hSpCas9	negative selection	9765731
100177920	100177943	8	+	27869803	PATU8988S	viability after 35 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.3173011056092899	[1004]	[431,576,NaN]	3	hSpCas9	negative selection	9848175
100183409	100183432	8	+	27869803	PATU8988S	viability after 35 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.2636433315805713	[1133]	[457,638,NaN]	3	hSpCas9	negative selection	9848176
100187217	100187240	8	+	27869803	PATU8988S	viability after 35 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.36561769932383437	[352]	[41,178,NaN]	-4	hSpCas9	negative selection	9848177
100191475	100191498	8	+	27869803	PATU8988S	viability after 35 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.42068298157267237	[330]	[139,58,NaN]	-4	hSpCas9	negative selection	9848178
100213348	100213371	8	+	27869803	PATU8988S	viability after 35 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.12755922591444002	[926]	[347,467,NaN]	1	hSpCas9	negative selection	9848179
100220302	100220325	8	+	27869803	PATU8988S	viability after 35 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.35892438706697827	[447]	[186,275,NaN]	4	hSpCas9	negative selection	9848180
100177920	100177943	8	+	27869803	PATU8988S	viability after 31 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	0.4796475146959024	[1004]	[505,625,NaN]	6	hSpCas9	negative selection	9930624
100183409	100183432	8	+	27869803	PATU8988S	viability after 31 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	0.24183773199134806	[1133]	[418,681,NaN]	3	hSpCas9	negative selection	9930625
100187217	100187240	8	+	27869803	PATU8988S	viability after 31 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	-0.7149447281372981	[352]	[50,129,NaN]	-6	hSpCas9	negative selection	9930626
100191475	100191498	8	+	27869803	PATU8988S	viability after 31 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.41423588408511225	[330]	[113,79,NaN]	-4	hSpCas9	negative selection	9930627
100213348	100213371	8	+	27869803	PATU8988S	viability after 31 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	-0.1306477109758002	[926]	[301,382,NaN]	-1	hSpCas9	negative selection	9930628
100220302	100220325	8	+	27869803	PATU8988S	viability after 31 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	0.3241139420405811	[447]	[198,254,NaN]	4	hSpCas9	negative selection	9930629
100177920	100177943	8	+	27869803	HPAFII	viability after 35 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.5240724214713495	[456]	[166,153,NaN]	-3	hSpCas9	negative selection	10013073
100183409	100183432	8	+	27869803	HPAFII	viability after 35 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	1.0810361222454141	[588]	[730,517,NaN]	7	hSpCas9	negative selection	10013074
100187217	100187240	8	+	27869803	HPAFII	viability after 35 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	0.7632189860099642	[141]	[145,94,NaN]	5	hSpCas9	negative selection	10013075
100191475	100191498	8	+	27869803	HPAFII	viability after 35 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-1.2929039071650021	[171]	[51,16,NaN]	-6	hSpCas9	negative selection	10013076
100213348	100213371	8	+	27869803	HPAFII	viability after 35 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.9949708190540938	[311]	[263,371,NaN]	6	hSpCas9	negative selection	10013077
100220302	100220325	8	+	27869803	HPAFII	viability after 35 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.4464473539292392	[233]	[69,105,NaN]	-3	hSpCas9	negative selection	10013078
100177920	100177943	8	+	27869803	HPAFII	viability after 31 days	CAGCTGTCTTCATGTAGGCAAGG	SPAG1	ENSG00000104450	-0.443930469913844	[456]	[222,150,NaN]	-3	hSpCas9	negative selection	10095522
100183409	100183432	8	+	27869803	HPAFII	viability after 31 days	CTCCAAGGGATTACGCGGAATGG	SPAG1	ENSG00000104450	1.1089478292673431	[588]	[823,591,NaN]	7	hSpCas9	negative selection	10095523
100187217	100187240	8	+	27869803	HPAFII	viability after 31 days	AAGGTCTTGGAGTTAGAACCTGG	SPAG1	ENSG00000104450	1.474880012809396	[141]	[232,208,NaN]	9	hSpCas9	negative selection	10095524
100191475	100191498	8	+	27869803	HPAFII	viability after 31 days	GCCTGATAATGATTTGGCCAAGG	SPAG1	ENSG00000104450	-0.7427513766403335	[171]	[72,40,NaN]	-5	hSpCas9	negative selection	10095525
100213348	100213371	8	+	27869803	HPAFII	viability after 31 days	GCAACGAGCTGTTCCGAAGCGGG	SPAG1	ENSG00000104450	0.7954896619821814	[311]	[167,451,NaN]	6	hSpCas9	negative selection	10095526
100220302	100220325	8	+	27869803	HPAFII	viability after 31 days	CTATGAAACCTCTTCTGAGGCGG	SPAG1	ENSG00000104450	-0.7204857008050373	[233]	[72,86,NaN]	-5	hSpCas9	negative selection	10095527
100240420	100240443	8	+	28162770	P31FUJ	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.7424765778312976	[83]	[58]	-5	hSpCas9	negative selection	10241065
100194205	100194228	8	+	28162770	P31FUJ	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.6171723515810138	[80]	[145]	7	hSpCas9	negative selection	10241066
100194157	100194180	8	+	28162770	P31FUJ	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.4275054212224195	[285]	[451]	5	hSpCas9	negative selection	10241067
100194238	100194261	8	+	28162770	P31FUJ	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.41253291428625927	[38]	[60]	4	hSpCas9	negative selection	10241068
100213234	100213257	8	+	28162770	P31FUJ	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.25442349461098057	[228]	[320]	2	hSpCas9	negative selection	10241069
100213361	100213384	8	+	28162770	P31FUJ	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	1.4820405788536875	[45]	[150]	9	hSpCas9	negative selection	10241070
100213863	100213886	8	+	28162770	P31FUJ	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.5143411747558595	[338]	[568]	6	hSpCas9	negative selection	10241071
100239302	100239325	8	+	28162770	P31FUJ	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.8741129995021333	[194]	[419]	8	hSpCas9	negative selection	10241072
100240399	100240422	8	+	28162770	P31FUJ	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.03507183731219987	[353]	[405]	0	hSpCas9	negative selection	10241073
100240672	100240695	8	-	28162770	P31FUJ	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.17624485702208248	[542]	[720]	1	hSpCas9	negative selection	10241074
100240420	100240443	8	+	28162770	TF1	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	NaN	[NaN]	[74]	1	hSpCas9	negative selection	10410431
100194205	100194228	8	+	28162770	TF1	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[159]	1	hSpCas9	negative selection	10410432
100194157	100194180	8	+	28162770	TF1	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[222]	1	hSpCas9	negative selection	10410433
100194238	100194261	8	+	28162770	TF1	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	NaN	[NaN]	[47]	1	hSpCas9	negative selection	10410434
100213234	100213257	8	+	28162770	TF1	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	NaN	[NaN]	[201]	1	hSpCas9	negative selection	10410435
100213361	100213384	8	+	28162770	TF1	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[45]	1	hSpCas9	negative selection	10410436
100213863	100213886	8	+	28162770	TF1	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[329]	1	hSpCas9	negative selection	10410437
100239302	100239325	8	+	28162770	TF1	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[170]	1	hSpCas9	negative selection	10410438
100240399	100240422	8	+	28162770	TF1	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[307]	1	hSpCas9	negative selection	10410439
100240672	100240695	8	-	28162770	TF1	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	NaN	[NaN]	[317]	1	hSpCas9	negative selection	10410440
100240420	100240443	8	+	28162770	SKM1	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.2537179384474797	[28]	[42]	-2	hSpCas9	negative selection	10579797
100194205	100194228	8	+	28162770	SKM1	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.26546114322833403	[23]	[50]	2	hSpCas9	negative selection	10579798
100194157	100194180	8	+	28162770	SKM1	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.1549146888649533	[62]	[123]	1	hSpCas9	negative selection	10579799
100194238	100194261	8	+	28162770	SKM1	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	-0.014646775964401337	[15]	[27]	0	hSpCas9	negative selection	10579800
100213234	100213257	8	+	28162770	SKM1	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.4951884778586937	[60]	[151]	5	hSpCas9	negative selection	10579801
100213361	100213384	8	+	28162770	SKM1	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.5382087320214142	[22]	[27]	-4	hSpCas9	negative selection	10579802
100213863	100213886	8	+	28162770	SKM1	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.5919960925756824	[80]	[94]	-5	hSpCas9	negative selection	10579803
100239302	100239325	8	+	28162770	SKM1	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.8683138028453792	[43]	[141]	8	hSpCas9	negative selection	10579804
100240399	100240422	8	+	28162770	SKM1	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.9628642338618553	[85]	[77]	-6	hSpCas9	negative selection	10579805
100240672	100240695	8	-	28162770	SKM1	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.8279805945495605	[93]	[294]	7	hSpCas9	negative selection	10579806
100240420	100240443	8	+	28162770	SKM1	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.2537179384474797	[28]	[42]	-2	hSpCas9	negative selection	10749163
100194205	100194228	8	+	28162770	SKM1	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.26546114322833403	[23]	[50]	2	hSpCas9	negative selection	10749164
100194157	100194180	8	+	28162770	SKM1	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.1549146888649533	[62]	[123]	1	hSpCas9	negative selection	10749165
100194238	100194261	8	+	28162770	SKM1	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	-0.014646775964401337	[15]	[27]	0	hSpCas9	negative selection	10749166
100213234	100213257	8	+	28162770	SKM1	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.4951884778586937	[60]	[151]	5	hSpCas9	negative selection	10749167
100213361	100213384	8	+	28162770	SKM1	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.5382087320214142	[22]	[27]	-4	hSpCas9	negative selection	10749168
100213863	100213886	8	+	28162770	SKM1	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.5919960925756824	[80]	[94]	-5	hSpCas9	negative selection	10749169
100239302	100239325	8	+	28162770	SKM1	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.8683138028453792	[43]	[141]	8	hSpCas9	negative selection	10749170
100240399	100240422	8	+	28162770	SKM1	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.9628642338618553	[85]	[77]	-6	hSpCas9	negative selection	10749171
100240672	100240695	8	-	28162770	SKM1	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.8279805945495605	[93]	[294]	7	hSpCas9	negative selection	10749172
100240420	100240443	8	+	28162770	PL21	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-1.2693456688119	[143]	[25]	-7	hSpCas9	negative selection	10918529
100194205	100194228	8	+	28162770	PL21	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.16451570475859878	[40]	[19]	1	hSpCas9	negative selection	10918530
100194157	100194180	8	+	28162770	PL21	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.9886355092956085	[263]	[227]	8	hSpCas9	negative selection	10918531
100194238	100194261	8	+	28162770	PL21	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	-0.1422525829577579	[70]	[27]	-1	hSpCas9	negative selection	10918532
100213234	100213257	8	+	28162770	PL21	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	-0.8341302875954257	[212]	[51]	-5	hSpCas9	negative selection	10918533
100213361	100213384	8	+	28162770	PL21	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-1.7204259180162746	[52]	[6]	-8	hSpCas9	negative selection	10918534
100213863	100213886	8	+	28162770	PL21	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.3599200585260893	[221]	[123]	3	hSpCas9	negative selection	10918535
100239302	100239325	8	+	28162770	PL21	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	1.5386642199073775	[120]	[152]	9	hSpCas9	negative selection	10918536
100240399	100240422	8	+	28162770	PL21	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.31507538133398005	[204]	[110]	3	hSpCas9	negative selection	10918537
100240672	100240695	8	-	28162770	PL21	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.023457246871699144	[320]	[141]	0	hSpCas9	negative selection	10918538
100240420	100240443	8	+	28162770	P31FUJ	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.7424765778312976	[83]	[58]	-5	hSpCas9	negative selection	11087895
100194205	100194228	8	+	28162770	P31FUJ	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.6171723515810138	[80]	[145]	7	hSpCas9	negative selection	11087896
100194157	100194180	8	+	28162770	P31FUJ	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.4275054212224195	[285]	[451]	5	hSpCas9	negative selection	11087897
100194238	100194261	8	+	28162770	P31FUJ	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.41253291428625927	[38]	[60]	4	hSpCas9	negative selection	11087898
100213234	100213257	8	+	28162770	P31FUJ	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.25442349461098057	[228]	[320]	2	hSpCas9	negative selection	11087899
100213361	100213384	8	+	28162770	P31FUJ	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	1.4820405788536875	[45]	[150]	9	hSpCas9	negative selection	11087900
100213863	100213886	8	+	28162770	P31FUJ	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.5143411747558595	[338]	[568]	6	hSpCas9	negative selection	11087901
100239302	100239325	8	+	28162770	P31FUJ	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.8741129995021333	[194]	[419]	8	hSpCas9	negative selection	11087902
100240399	100240422	8	+	28162770	P31FUJ	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.03507183731219987	[353]	[405]	0	hSpCas9	negative selection	11087903
100240672	100240695	8	-	28162770	P31FUJ	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.17624485702208248	[542]	[720]	1	hSpCas9	negative selection	11087904
100240420	100240443	8	+	28162770	OCIAML5	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.3590265595838802	[53]	[75]	-3	hSpCas9	negative selection	11257261
100194205	100194228	8	+	28162770	OCIAML5	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.06945937111291839	[84]	[160]	0	hSpCas9	negative selection	11257262
100194157	100194180	8	+	28162770	OCIAML5	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.7244836900409592	[171]	[512]	6	hSpCas9	negative selection	11257263
100194238	100194261	8	+	28162770	OCIAML5	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	-0.893886746558624	[34]	[33]	-5	hSpCas9	negative selection	11257264
100213234	100213257	8	+	28162770	OCIAML5	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.7439089225795771	[86]	[262]	6	hSpCas9	negative selection	11257265
100213361	100213384	8	+	28162770	OCIAML5	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.11510097669779062	[26]	[44]	-1	hSpCas9	negative selection	11257266
100213863	100213886	8	+	28162770	OCIAML5	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.22588140741632767	[229]	[354]	-2	hSpCas9	negative selection	11257267
100239302	100239325	8	+	28162770	OCIAML5	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.43744004633098804	[89]	[219]	4	hSpCas9	negative selection	11257268
100240399	100240422	8	+	28162770	OCIAML5	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.14107975809494067	[210]	[419]	1	hSpCas9	negative selection	11257269
100240672	100240695	8	-	28162770	OCIAML5	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.665257212981796	[261]	[749]	6	hSpCas9	negative selection	11257270
100240420	100240443	8	+	28162770	EOL1	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.5682210309408752	[196]	[69]	-4	hSpCas9	negative selection	11426627
100194205	100194228	8	+	28162770	EOL1	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	3.4222181944955237	[87]	[496]	9	hSpCas9	negative selection	11426628
100194157	100194180	8	+	28162770	EOL1	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.672360747973733	[529]	[444]	6	hSpCas9	negative selection	11426629
100194238	100194261	8	+	28162770	EOL1	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.2020817470994436	[98]	[59]	1	hSpCas9	negative selection	11426630
100213234	100213257	8	+	28162770	EOL1	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	-0.40127423942131885	[370]	[147]	-3	hSpCas9	negative selection	11426631
100213361	100213384	8	+	28162770	EOL1	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-1.162915069679805	[101]	[23]	-7	hSpCas9	negative selection	11426632
100213863	100213886	8	+	28162770	EOL1	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.216998665381063	[355]	[217]	2	hSpCas9	negative selection	11426633
100239302	100239325	8	+	28162770	EOL1	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.9245477715705345	[181]	[181]	7	hSpCas9	negative selection	11426634
100240399	100240422	8	+	28162770	EOL1	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.17203117386981287	[341]	[202]	1	hSpCas9	negative selection	11426635
100240672	100240695	8	-	28162770	EOL1	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.951732600896765	[367]	[374]	7	hSpCas9	negative selection	11426636
100240420	100240443	8	+	28162770	MOLM13	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-2.615526626643059	[177]	[20]	-8	hSpCas9	negative selection	11595993
100194205	100194228	8	+	28162770	MOLM13	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	-0.06886402613395748	[102]	[70]	0	hSpCas9	negative selection	11595994
100194157	100194180	8	+	28162770	MOLM13	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.05407502353517596	[392]	[294]	0	hSpCas9	negative selection	11595995
100194238	100194261	8	+	28162770	MOLM13	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.6730038114491912	[71]	[82]	5	hSpCas9	negative selection	11595996
100213234	100213257	8	+	28162770	MOLM13	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.6559617296971507	[236]	[269]	5	hSpCas9	negative selection	11595997
100213361	100213384	8	+	28162770	MOLM13	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-3.254576642926512	[65]	[4]	-8	hSpCas9	negative selection	11595998
100213863	100213886	8	+	28162770	MOLM13	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-1.7848186642193409	[528]	[110]	-7	hSpCas9	negative selection	11595999
100239302	100239325	8	+	28162770	MOLM13	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	-0.34111339339450764	[218]	[124]	-2	hSpCas9	negative selection	11596000
100240399	100240422	8	+	28162770	MOLM13	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.05019253581524513	[361]	[270]	0	hSpCas9	negative selection	11596001
100240672	100240695	8	-	28162770	MOLM13	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.45043721258522995	[498]	[492]	3	hSpCas9	negative selection	11596002
100240420	100240443	8	+	28162770	HEL	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.06213313245599594	[162]	[99]	0	hSpCas9	negative selection	11765359
100194205	100194228	8	+	28162770	HEL	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.7523633231748551	[75]	[81]	6	hSpCas9	negative selection	11765360
100194157	100194180	8	+	28162770	HEL	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	1.041017813017834	[367]	[484]	8	hSpCas9	negative selection	11765361
100194238	100194261	8	+	28162770	HEL	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	1.3357610785789662	[59]	[96]	9	hSpCas9	negative selection	11765362
100213234	100213257	8	+	28162770	HEL	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	1.019302183464565	[284]	[369]	8	hSpCas9	negative selection	11765363
100213361	100213384	8	+	28162770	HEL	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	0.6020968475030108	[71]	[69]	5	hSpCas9	negative selection	11765364
100213863	100213886	8	+	28162770	HEL	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.606041590153195	[422]	[177]	-4	hSpCas9	negative selection	11765365
100239302	100239325	8	+	28162770	HEL	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	1.2509711120443603	[163]	[249]	9	hSpCas9	negative selection	11765366
100240399	100240422	8	+	28162770	HEL	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.346174802601369	[295]	[240]	3	hSpCas9	negative selection	11765367
100240672	100240695	8	-	28162770	HEL	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	-0.28880586580154943	[390]	[204]	-2	hSpCas9	negative selection	11765368
100240420	100240443	8	+	28162770	MV411	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-2.4750284736883157	[131]	[19]	-8	hSpCas9	negative selection	11934725
100194205	100194228	8	+	28162770	MV411	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.4049788277692554	[51]	[57]	3	hSpCas9	negative selection	11934726
100194157	100194180	8	+	28162770	MV411	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.7517122059383705	[238]	[338]	6	hSpCas9	negative selection	11934727
100194238	100194261	8	+	28162770	MV411	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.2760067029795463	[49]	[50]	2	hSpCas9	negative selection	11934728
100213234	100213257	8	+	28162770	MV411	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.5455189037157699	[182]	[224]	5	hSpCas9	negative selection	11934729
100213361	100213384	8	+	28162770	MV411	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.39892549460252424	[71]	[45]	-3	hSpCas9	negative selection	11934730
100213863	100213886	8	+	28162770	MV411	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	-0.6441862881724327	[281]	[151]	-4	hSpCas9	negative selection	11934731
100239302	100239325	8	+	28162770	MV411	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	1.0262233206436087	[101]	[174]	8	hSpCas9	negative selection	11934732
100240399	100240422	8	+	28162770	MV411	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.0007975831996499538	[279]	[235]	0	hSpCas9	negative selection	11934733
100240672	100240695	8	-	28162770	MV411	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	0.4079022229760215	[288]	[322]	3	hSpCas9	negative selection	11934734
100240420	100240443	8	+	28162770	MONOMAC1	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.42921028918858717	[73]	[62]	-4	hSpCas9	negative selection	12104091
100194205	100194228	8	+	28162770	MONOMAC1	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	1.213595473099289	[40]	[108]	9	hSpCas9	negative selection	12104092
100194157	100194180	8	+	28162770	MONOMAC1	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	-0.09918952366140898	[170]	[182]	-1	hSpCas9	negative selection	12104093
100194238	100194261	8	+	28162770	MONOMAC1	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.7098537485489649	[55]	[104]	6	hSpCas9	negative selection	12104094
100213234	100213257	8	+	28162770	MONOMAC1	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	1.5922841521677886	[137]	[476]	9	hSpCas9	negative selection	12104095
100213361	100213384	8	+	28162770	MONOMAC1	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.14973113228119717	[29]	[30]	-1	hSpCas9	negative selection	12104096
100213863	100213886	8	+	28162770	MONOMAC1	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	1.0097755628094363	[132]	[306]	8	hSpCas9	negative selection	12104097
100239302	100239325	8	+	28162770	MONOMAC1	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	1.5998712875629346	[98]	[343]	9	hSpCas9	negative selection	12104098
100240399	100240422	8	+	28162770	MONOMAC1	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	-0.34590023297403644	[152]	[137]	-3	hSpCas9	negative selection	12104099
100240672	100240695	8	-	28162770	MONOMAC1	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	-0.2713841885196351	[218]	[207]	-2	hSpCas9	negative selection	12104100
100240420	100240443	8	+	28162770	OCIAML2	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.6512020708839821	[16]	[59]	-5	hSpCas9	negative selection	12273457
100194205	100194228	8	+	28162770	OCIAML2	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	0.8221519239856847	[9]	[97]	7	hSpCas9	negative selection	12273458
100194157	100194180	8	+	28162770	OCIAML2	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.36731341664886596	[59]	[428]	3	hSpCas9	negative selection	12273459
100194238	100194261	8	+	28162770	OCIAML2	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	1.1649587485489628	[6]	[86]	9	hSpCas9	negative selection	12273460
100213234	100213257	8	+	28162770	OCIAML2	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.49284429873272473	[29]	[233]	5	hSpCas9	negative selection	12273461
100213361	100213384	8	+	28162770	OCIAML2	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.996698636909749	[17]	[49]	-6	hSpCas9	negative selection	12273462
100213863	100213886	8	+	28162770	OCIAML2	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.014797001928080478	[39]	[223]	0	hSpCas9	negative selection	12273463
100239302	100239325	8	+	28162770	OCIAML2	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	1.0685289858658702	[23]	[278]	8	hSpCas9	negative selection	12273464
100240399	100240422	8	+	28162770	OCIAML2	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.43885788185239644	[36]	[277]	4	hSpCas9	negative selection	12273465
100240672	100240695	8	-	28162770	OCIAML2	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	1.218080600316824	[37]	[489]	9	hSpCas9	negative selection	12273466
100240420	100240443	8	+	28162770	OCIAML3	viability	GGAGCCTGGAAGACCTGCAGGGG	SPAG1	ENSG00000104450	-0.8055117455178749	[32]	[56]	-5	hSpCas9	negative selection	12442823
100194205	100194228	8	+	28162770	OCIAML3	viability	GAAAACTCCGAAGATGAAGAAGG	SPAG1	ENSG00000104450	1.1117070198576031	[20]	[136]	8	hSpCas9	negative selection	12442824
100194157	100194180	8	+	28162770	OCIAML3	viability	GCATCTGAGACTCAAACCAAAGG	SPAG1	ENSG00000104450	0.3585451972733765	[84]	[328]	3	hSpCas9	negative selection	12442825
100194238	100194261	8	+	28162770	OCIAML3	viability	AGAAAACATGAAGATGGCGGTGG	SPAG1	ENSG00000104450	0.7279204545631992	[13]	[69]	6	hSpCas9	negative selection	12442826
100213234	100213257	8	+	28162770	OCIAML3	viability	CGGACAAGCGGAGCCCACGGCGG	SPAG1	ENSG00000104450	0.35660433271367337	[51]	[200]	3	hSpCas9	negative selection	12442827
100213361	100213384	8	+	28162770	OCIAML3	viability	CCGAAGCGGGCAGTTCGCCGAGG	SPAG1	ENSG00000104450	-0.8439858933325106	[21]	[36]	-6	hSpCas9	negative selection	12442828
100213863	100213886	8	+	28162770	OCIAML3	viability	GCAGCATGTTACCTAAAAGAAGG	SPAG1	ENSG00000104450	0.283864061812818	[76]	[282]	2	hSpCas9	negative selection	12442829
100239302	100239325	8	+	28162770	OCIAML3	viability	GGCAAAGATGGAACTGGAAGAGG	SPAG1	ENSG00000104450	0.14033248443521973	[54]	[182]	1	hSpCas9	negative selection	12442830
100240399	100240422	8	+	28162770	OCIAML3	viability	GAAGGTGAATGAAGGCAAGGAGG	SPAG1	ENSG00000104450	0.18950053977907325	[60]	[209]	1	hSpCas9	negative selection	12442831
100240672	100240695	8	-	28162770	OCIAML3	viability	TCAGAGACTGAATGAGGAGAAGG	SPAG1	ENSG00000104450	1.3858144777382067	[107]	[851]	9	hSpCas9	negative selection	12442832